FastQCFastQC Report
Fri 27 May 2016
SRR939012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4500857
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT134860.29963182567231084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91000.20218371745647554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85310.18954168061771345No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT56520.12557608473230764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52530.11671110635152374No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45510.10111407671916704No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG113400.022.302342
GTACATG116250.022.2134911
ACATGGG114950.020.8045253
AGAGTAC149950.018.87767410-11
GAGTACA93300.018.7410931
GAGTACT107850.018.48070112-13
AGTACAT84800.017.9616172
GTACTTT111900.017.62098914-15
AGTACTT114150.017.37759612-13
CCTATAC11400.017.0653513
CATGGGG76100.016.648034
GTATCAA251300.015.8877351
GTGTAGC24950.015.8495911
TAGACAG22400.015.4636175
GGTATCA219850.015.0615221
TACTTTT135400.014.56269314-15
TCTAGAC28700.014.38383
CAACGCA275050.014.3014195
ATCAACG277200.014.2076143
GTATAGG12100.014.1751491
Produced by FastQC (version 0.11.5)