##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939012_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4500857 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14673561057372 33.0 31.0 34.0 30.0 34.0 2 32.379505503063086 34.0 31.0 34.0 30.0 34.0 3 32.48492164936589 34.0 31.0 34.0 30.0 34.0 4 36.05684139709393 37.0 35.0 37.0 35.0 37.0 5 35.96851310761484 37.0 35.0 37.0 35.0 37.0 6 36.00311940592647 37.0 35.0 37.0 35.0 37.0 7 35.93967148922972 37.0 35.0 37.0 35.0 37.0 8 35.904907665362394 37.0 35.0 37.0 35.0 37.0 9 37.58671559660749 39.0 37.0 39.0 35.0 39.0 10-11 37.50238454587648 39.0 37.0 39.0 34.5 39.0 12-13 37.430142192920144 39.0 37.0 39.0 34.0 39.0 14-15 38.70640624663258 40.0 38.0 41.0 34.0 41.0 16-17 38.693844194561166 40.0 38.0 41.0 34.0 41.0 18-19 38.7715590608633 40.0 38.0 41.0 34.0 41.0 20-21 38.75858142127154 40.0 38.0 41.0 34.0 41.0 22-23 38.695273144647786 40.0 38.0 41.0 34.0 41.0 24-25 38.63888121751035 40.0 38.0 41.0 34.0 41.0 26-27 38.482771614383665 40.0 38.0 41.0 34.0 41.0 28-29 38.3116325402029 40.0 38.0 41.0 34.0 41.0 30-31 38.164345034734495 40.0 38.0 41.0 33.0 41.0 32-33 38.03292661819738 40.0 37.5 41.0 33.0 41.0 34-35 38.02805032463817 40.0 38.0 41.0 33.0 41.0 36-37 37.942569381786626 40.0 37.0 41.0 33.0 41.0 38-39 37.82097698282794 40.0 37.0 41.0 33.0 41.0 40-41 37.686280634999065 40.0 37.0 41.0 33.0 41.0 42-43 37.5316455288404 40.0 37.0 41.0 32.0 41.0 44-45 37.30722004720434 40.0 37.0 41.0 31.5 41.0 46-47 37.05851974412873 40.0 36.0 41.0 31.0 41.0 48-49 36.80778805014245 39.5 36.0 41.0 30.5 41.0 50-51 36.54892134986737 39.0 35.5 41.0 30.0 41.0 52-53 36.32359959447723 39.0 35.0 40.5 30.0 41.0 54-55 36.06915171932812 39.0 35.0 40.0 29.0 41.0 56-57 35.76664744069852 38.0 35.0 40.0 28.5 41.0 58-59 35.44560391498774 38.0 34.5 40.0 28.0 41.0 60-61 35.01203148644802 37.5 34.0 40.0 27.5 41.0 62-63 34.68596680587719 37.0 34.0 40.0 27.0 41.0 64-65 34.40905976350726 37.0 34.0 39.5 26.5 41.0 66-67 33.94057731671991 36.0 33.0 39.0 26.0 40.5 68-69 33.51066463564605 35.5 33.0 39.0 26.0 40.0 70-71 33.03903245537461 35.0 32.0 38.0 25.0 40.0 72-73 32.51762131078593 35.0 32.0 37.0 24.0 39.5 74-75 32.0479559781615 35.0 31.0 37.0 22.5 39.0 76-77 30.095216644296855 33.0 29.0 35.0 20.0 37.0 78-79 31.08066352696831 34.0 30.5 36.0 21.0 38.0 80-81 31.09048698947778 34.0 31.0 35.5 20.5 37.0 82-83 30.76314155281983 34.0 31.0 35.0 20.0 37.0 84-85 30.358696354938626 34.0 30.0 35.0 18.5 36.0 86-87 29.767331421549272 34.0 29.5 35.0 13.5 36.0 88-89 29.183969519582604 34.0 29.0 35.0 7.0 35.5 90-91 28.703233628617838 33.5 29.0 35.0 2.0 35.0 92-93 28.321641078576814 33.0 29.0 35.0 2.0 35.0 94-95 27.893056811180628 33.0 27.5 35.0 2.0 35.0 96-97 27.218911420647224 33.0 26.5 35.0 2.0 35.0 98-99 26.254857019452075 33.0 25.0 34.0 2.0 35.0 100-101 21.823898981905003 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17383365228247527 1101 2 0.14229981494745658 1101 3 0.16366768750315686 1101 4 0.10735135472641133 1101 5 0.1387464439027326 1101 6 0.12009238614495388 1101 7 0.09576719181257687 1101 8 0.15284500387259925 1101 9 0.1881897796016787 1101 10-11 0.2509299665139082 1101 12-13 0.18992851148475864 1101 14-15 0.22908313374495748 1101 16-17 0.3038847613629656 1101 18-19 0.26985045347573333 1101 20-21 0.2581975413963491 1101 22-23 0.27552807718969774 1101 24-25 0.2931369129780421 1101 26-27 0.22563716363505648 1101 28-29 0.2927824232220786 1101 30-31 0.2686918569243275 1101 32-33 0.2707012235297057 1101 34-35 0.3560510734661477 1101 36-37 0.2992597768213727 1101 38-39 0.3702003412974264 1101 40-41 0.3791980903161303 1101 42-43 0.3178397019911756 1101 44-45 0.3227332602424795 1101 46-47 0.28990716768107916 1101 48-49 0.04993818827441743 1101 50-51 0.14546365637043124 1101 52-53 0.173212149989304 1101 54-55 0.138016896206139 1101 56-57 -0.011816653641098185 1101 58-59 0.09112927841862728 1101 60-61 0.0771832026915078 1101 62-63 0.11182387819430062 1101 64-65 0.08107554796523431 1101 66-67 0.13343564584982914 1101 68-69 0.19346480914335729 1101 70-71 0.1796870836839375 1101 72-73 0.021927495089428817 1101 74-75 0.1113695947726896 1101 76-77 0.005977050640503023 1101 78-79 0.20905905821078363 1101 80-81 0.3077996911509828 1101 82-83 0.43876597147720275 1101 84-85 0.3700586044665215 1101 86-87 0.5361394306919465 1101 88-89 0.5399402033113283 1101 90-91 0.53467821484967 1101 92-93 0.5652690398250932 1101 94-95 0.46591433261624715 1101 96-97 0.4456986260647753 1101 98-99 0.40625548994918503 1101 100-101 0.9507216819549882 1102 1 0.08557828282073388 1102 2 0.06210486773611734 1102 3 0.11701450267506885 1102 4 0.1255310077137466 1102 5 0.09864847955930145 1102 6 0.14961582416210462 1102 7 0.13635556675847482 1102 8 0.18098183508291044 1102 9 0.16779739005011862 1102 10-11 0.21664473642279347 1102 12-13 0.2563772124310191 1102 14-15 0.31058810540920945 1102 16-17 0.21595044344974212 1102 18-19 0.13572642528644252 1102 20-21 0.19141061307994534 1102 22-23 0.17280071484081816 1102 24-25 0.15806000837299194 1102 26-27 0.22556944182404237 1102 28-29 0.2525665454999597 1102 30-31 0.2527766860717975 1102 32-33 0.28281197715350714 1102 34-35 0.22206995901814253 1102 36-37 0.28551966477830604 1102 38-39 0.2789410296847308 1102 40-41 0.3092719125427763 1102 42-43 0.25634448074096383 1102 44-45 0.27480527502743257 1102 46-47 0.35284177731064403 1102 48-49 0.21189830721615266 1102 50-51 0.3914041645588284 1102 52-53 0.31269958748431037 1102 54-55 0.22107704309812704 1102 56-57 0.25725529691172966 1102 58-59 0.20189756982350815 1102 60-61 0.07880231014420502 1102 62-63 0.19127331295710093 1102 64-65 0.13411500317216252 1102 66-67 0.2683188180921334 1102 68-69 0.34889498059512647 1102 70-71 0.35117563962482023 1102 72-73 0.31086865421420384 1102 74-75 0.31746091539925914 1102 76-77 0.08961070639581692 1102 78-79 0.18240103797995033 1102 80-81 0.4242414290736818 1102 82-83 0.36700187173616783 1102 84-85 0.28524783240188967 1102 86-87 0.3623311846181494 1102 88-89 0.5415598015017942 1102 90-91 0.47763717488692947 1102 92-93 0.6730396868802764 1102 94-95 0.7187104473254351 1102 96-97 0.6518573413438524 1102 98-99 0.6132266215915543 1102 100-101 0.8892098574105312 1103 1 0.06645519975973713 1103 2 0.08090205023941621 1103 3 0.11739094910951309 1103 4 0.045932109838503266 1103 5 0.04233620082120382 1103 6 0.014675288845431567 1103 7 0.11755324943595014 1103 8 0.11401657154660683 1103 9 0.11338371072054088 1103 10-11 0.1054063661865996 1103 12-13 0.088353010102459 1103 14-15 0.21235197999831001 1103 16-17 0.1603014656825934 1103 18-19 0.13271244686435324 1103 20-21 0.18329623476774515 1103 22-23 0.2236486366323831 1103 24-25 0.267734318021958 1103 26-27 0.18673263777190385 1103 28-29 0.22161222383552115 1103 30-31 0.3152736138720158 1103 32-33 0.35034022547892363 1103 34-35 0.2262022047896295 1103 36-37 0.10342100318499092 1103 38-39 0.2316771047369599 1103 40-41 0.3042420629293261 1103 42-43 0.2569882319574788 1103 44-45 0.2771784975442273 1103 46-47 0.49854168446134395 1103 48-49 0.3033516573567496 1103 50-51 0.2914552292662691 1103 52-53 0.4082952390840475 1103 54-55 0.41883775913154864 1103 56-57 0.36137972278143593 1103 58-59 0.2581419191822718 1103 60-61 0.2832835952525343 1103 62-63 0.3145423506871623 1103 64-65 0.33756154197504884 1103 66-67 0.4670643764066398 1103 68-69 0.42108259597812747 1103 70-71 0.3687562443856436 1103 72-73 0.33782565283106436 1103 74-75 0.3641952318828814 1103 76-77 0.3584237881180137 1103 78-79 0.4367813998210366 1103 80-81 0.526900452419298 1103 82-83 0.4786477112729628 1103 84-85 0.5523870967759201 1103 86-87 0.5010528456979344 1103 88-89 0.8022270541440442 1103 90-91 0.7289047130358313 1103 92-93 0.6536439235233438 1103 94-95 0.5798769167205471 1103 96-97 0.46877231306998723 1103 98-99 0.6707641163357145 1103 100-101 1.096327524520948 1104 1 -0.00657856486461128 1104 2 -0.023373675846713127 1104 3 0.022210953824661317 1104 4 0.02589558303819217 1104 5 0.04474370913261794 1104 6 -0.03451724585817573 1104 7 -0.0365660023628962 1104 8 -0.030569658261207167 1104 9 0.056907926480555204 1104 10-11 0.035175930603415395 1104 12-13 0.047601591815372046 1104 14-15 0.03798406201164539 1104 16-17 0.015512538422363775 1104 18-19 -0.005080112287792815 1104 20-21 0.030212289828888572 1104 22-23 -0.029437857893434227 1104 24-25 -0.01951949806703368 1104 26-27 -0.030872043269745575 1104 28-29 -0.015099733616203537 1104 30-31 -0.1307764385399821 1104 32-33 0.03109425048064196 1104 34-35 -8.127001909130627E-4 1104 36-37 0.001714620706636083 1104 38-39 0.06592731961371356 1104 40-41 0.07994968835776461 1104 42-43 0.004546592505356273 1104 44-45 0.07862938707766887 1104 46-47 0.11102895834617499 1104 48-49 0.12519416450419385 1104 50-51 0.00498668066941832 1104 52-53 -0.047750410794286324 1104 54-55 0.09961310121920519 1104 56-57 0.09517063115536217 1104 58-59 0.06668528884651437 1104 60-61 0.1891105016784067 1104 62-63 0.19598042140519567 1104 64-65 0.21392670164716776 1104 66-67 0.16338209577678242 1104 68-69 0.27228896584078655 1104 70-71 0.058728480864807864 1104 72-73 0.1634554074206207 1104 74-75 0.0903761521599975 1104 76-77 -0.08485871939335254 1104 78-79 0.16259508965362812 1104 80-81 0.27931450661007773 1104 82-83 0.32570679710427797 1104 84-85 0.22534517781078378 1104 86-87 0.19764686729737946 1104 88-89 0.43164377053735237 1104 90-91 0.38989557773882666 1104 92-93 0.30818689868925375 1104 94-95 0.29460389695220357 1104 96-97 0.268146981908 1104 98-99 0.34611494769942297 1104 100-101 0.9129646690512665 1105 1 -0.015028775787605753 1105 2 0.04802546129286611 1105 3 0.040307171010837806 1105 4 0.050574749102814565 1105 5 0.06665866280982868 1105 6 0.04812383159121936 1105 7 -0.009460823409206398 1105 8 0.016941296749479307 1105 9 -0.059853866799180366 1105 10-11 -0.004042101691254629 1105 12-13 0.03741903002322289 1105 14-15 0.1361931414688513 1105 16-17 0.09895895537416521 1105 18-19 0.11216623977581008 1105 20-21 0.11590069324637398 1105 22-23 0.05744088938322278 1105 24-25 -0.0018417469472851167 1105 26-27 -0.0322567454067908 1105 28-29 0.09047209013938584 1105 30-31 0.09655213691378606 1105 32-33 0.050561489182463504 1105 34-35 0.13796222534315206 1105 36-37 0.09836390164850428 1105 38-39 0.20926767878116692 1105 40-41 0.22757036870156355 1105 42-43 0.1386586586524885 1105 44-45 0.11800070982657473 1105 46-47 0.16238305171529532 1105 48-49 0.13027813781976505 1105 50-51 0.15535020878693473 1105 52-53 0.2859720198331459 1105 54-55 0.2507255070714791 1105 56-57 0.19550981192029582 1105 58-59 0.143600182557428 1105 60-61 0.11549803728761532 1105 62-63 0.1132469802917484 1105 64-65 0.07748080455898076 1105 66-67 0.17518867972249552 1105 68-69 0.149580956665055 1105 70-71 0.2422670226538699 1105 72-73 0.24265387460953747 1105 74-75 0.3197067450770916 1105 76-77 0.09357471810134399 1105 78-79 0.2383876272614458 1105 80-81 0.22459993862774752 1105 82-83 0.4014290106070142 1105 84-85 0.21868907524901005 1105 86-87 0.0031584288242747505 1105 88-89 0.16909886136655317 1105 90-91 0.3684853533252337 1105 92-93 0.4510572363555454 1105 94-95 0.4538546630540239 1105 96-97 0.35576001771784505 1105 98-99 0.4282485961041296 1105 100-101 0.8936434521623333 1106 1 -0.026360267951680783 1106 2 -0.1125476658327429 1106 3 -0.11186780370123017 1106 4 -0.05602891039802671 1106 5 -0.06638327451491932 1106 6 -0.02213906383988018 1106 7 -0.06983928096045844 1106 8 -0.04498744971718338 1106 9 -0.05318577918179557 1106 10-11 -0.02385435419608939 1106 12-13 -0.02177665288852637 1106 14-15 -0.0826540391236037 1106 16-17 -0.05226241344626459 1106 18-19 -0.18481436465132361 1106 20-21 -0.16252472483871827 1106 22-23 -0.027185598418910217 1106 24-25 0.0028484773701435984 1106 26-27 -0.05175997651930686 1106 28-29 0.02020120691605598 1106 30-31 1.9539928181444566E-4 1106 32-33 -0.06822001456897198 1106 34-35 -0.0040273284446143975 1106 36-37 -0.0742221824849878 1106 38-39 0.013832793038950797 1106 40-41 0.0667814195188825 1106 42-43 0.11199126263635151 1106 44-45 -0.021626429132297176 1106 46-47 -0.014295512043261738 1106 48-49 -0.14528176091524614 1106 50-51 -0.07217163833036011 1106 52-53 0.03375560971279157 1106 54-55 -0.057062858294585794 1106 56-57 -0.055149256771890975 1106 58-59 0.033729162146293845 1106 60-61 0.04857618762230942 1106 62-63 0.015601857273686903 1106 64-65 -0.04580164316749347 1106 66-67 -0.02575972904861601 1106 68-69 0.018790312857092317 1106 70-71 0.03407436883426129 1106 72-73 0.097701936852026 1106 74-75 0.2266403458969819 1106 76-77 -0.05512751338446975 1106 78-79 0.15560303334665093 1106 80-81 0.2514324999426485 1106 82-83 0.22216396939203165 1106 84-85 0.19830089281349572 1106 86-87 0.20103757898789354 1106 88-89 0.24242785567722436 1106 90-91 0.4216860212259981 1106 92-93 0.447665281053208 1106 94-95 0.512529066689499 1106 96-97 0.4572312932125513 1106 98-99 0.4447969002048424 1106 100-101 0.6758524043136376 1107 1 -0.0918847700181189 1107 2 -0.06465949052478237 1107 3 -0.09941966550483272 1107 4 -0.1263143561585096 1107 5 -0.14777588192357882 1107 6 -0.054111137789895736 1107 7 -0.0831210235973856 1107 8 -0.05981339889361692 1107 9 -0.06856418458948355 1107 10-11 -0.06299440215194352 1107 12-13 -0.056784876473649604 1107 14-15 -0.026172619003837383 1107 16-17 -0.060938596979809745 1107 18-19 -0.07463087528073942 1107 20-21 -0.06366337508632824 1107 22-23 -0.06982538932496851 1107 24-25 -0.11252224666682054 1107 26-27 -0.08391615234136651 1107 28-29 -0.15214455316812803 1107 30-31 -0.21751332314055816 1107 32-33 -0.054083882606029476 1107 34-35 -0.12207026580077951 1107 36-37 -0.0643660801631114 1107 38-39 -0.06423664253593842 1107 40-41 -0.21149717024169234 1107 42-43 -0.11437762791265271 1107 44-45 -0.25974023591921025 1107 46-47 -0.4120697721214128 1107 48-49 -0.31030275666187634 1107 50-51 -0.22820628382255137 1107 52-53 -0.1453105966740864 1107 54-55 -0.1309188361079734 1107 56-57 -0.07415121075194264 1107 58-59 -0.14785452563460666 1107 60-61 -0.22475484632031595 1107 62-63 -0.19194852850182542 1107 64-65 -0.15398049560082683 1107 66-67 -0.04414556681003745 1107 68-69 -0.03856743619001435 1107 70-71 -0.049901689387517933 1107 72-73 -0.08015282198304874 1107 74-75 -0.11714442109140322 1107 76-77 -0.37915368627455237 1107 78-79 -0.029115105532532937 1107 80-81 -0.06316762697568734 1107 82-83 -0.013789514636744826 1107 84-85 -0.062266865913141345 1107 86-87 -0.009945475807896287 1107 88-89 0.0483378922666553 1107 90-91 0.22295209185845977 1107 92-93 0.3455032011187562 1107 94-95 0.2946961872097056 1107 96-97 0.20657375783808263 1107 98-99 0.20848173908683876 1107 100-101 0.6857768356913816 1108 1 0.022789341672798002 1108 2 -0.007870960839305496 1108 3 -0.014080815802557822 1108 4 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6 181.0 7 468.0 8 1165.0 9 2145.0 10 3541.0 11 5407.0 12 7801.0 13 10548.0 14 13042.0 15 16706.0 16 21125.0 17 28515.0 18 28703.0 19 23000.0 20 22970.0 21 25234.0 22 27395.0 23 30995.0 24 35658.0 25 43002.0 26 52254.0 27 63705.0 28 77767.0 29 94690.0 30 114910.0 31 140719.0 32 174828.0 33 221017.0 34 298267.0 35 427886.0 36 675591.0 37 1027588.0 38 731428.0 39 52467.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01540843392577 19.13557057160895 13.612694390098627 24.236326604366653 2 16.264702477772566 20.580480561813005 38.17897347105229 24.975843489362138 3 20.046293405900254 23.110820894776264 29.49731573342588 27.345569965897603 4 13.551530297452242 16.132527649734264 35.66951804956256 34.646424003250935 5 14.132304963246895 36.810207152258556 34.47135692772289 14.586130956771665 6 33.83928882877194 35.25526360868608 16.373215145471185 14.532232417070793 7 29.366140714979394 31.65963726463649 20.503806275116048 18.470415745268067 8 26.44116442713021 35.33604822370495 19.341338771705033 18.881448577459803 9 25.816416740189702 16.42696046552912 20.04982606645801 37.70679672782317 10-11 25.7173245006451 25.941648446062604 26.899543797992248 21.441483255300046 12-13 26.020777820757246 23.106332860608546 27.488531628532076 23.384357690102128 14-15 22.719933611654845 24.837150811302017 25.52726299160814 26.915652585435 16-17 22.808257687632832 27.71478274239313 26.30572916545159 23.171230404522444 18-19 22.686934954831937 27.04607144817087 27.705601399911174 22.561392197086022 20-21 23.45297795508722 26.10728356844041 27.68009514632436 22.759643330148013 22-23 22.957705610287107 26.011079667716615 27.767556267617476 23.2636584543788 24-25 23.079537963547832 26.129579322337946 27.886255884157173 22.90462682995705 26-27 23.013395004551356 26.03588605458916 28.040637594129297 22.91008134673019 28-29 22.807745280509913 26.682596225563266 27.806582168684763 22.70307632524206 30-31 23.009840126002672 26.235692447016202 27.58999008411065 23.16447734287048 32-33 22.919024088079226 26.205820358211778 27.82847799874557 23.04667755496342 34-35 23.330714139944913 26.224165552870694 27.69408739588817 22.751032911296218 36-37 23.243801320054942 26.090888456812912 27.89235716208519 22.77295306104696 38-39 22.885100189434972 26.466476676166188 27.893160897510683 22.755262236888154 40-41 23.066299201821565 26.364350992323942 27.698673642692277 22.870676163162212 42-43 22.94262319576514 26.358445306087123 27.42736930229272 23.271562195855015 44-45 23.023280098825996 26.281094195750125 27.512942028599106 23.18268367682477 46-47 23.322541276174828 26.634922228978986 27.26700409990044 22.775532394945746 48-49 23.28852023688012 26.47752353892894 27.291026878970648 22.942929345220296 50-51 22.831516758762753 26.72846941710347 27.492829369374434 22.947184454759345 52-53 23.181051964103723 26.909308605005666 26.923050432395428 22.986588998495176 54-55 23.065118487434727 26.54865506724608 27.009322891173838 23.376903554145358 56-57 22.987833205987215 26.717056329494582 27.107648610031376 23.187461854486823 58-59 23.430517284972645 26.512515647058354 27.024974860281826 23.03199220768718 60-61 23.11953832444979 26.586159842950146 27.375708397947758 22.91859343465231 62-63 22.84904852564745 26.566696075880657 27.44907247664167 23.135182921830218 64-65 22.818636792898765 26.8037955945872 27.167704674417575 23.209862938096464 66-67 22.872334220921903 26.70502157241264 26.90337645445898 23.51926775220648 68-69 22.744329412224758 26.589250990053255 27.199557942111213 23.466861655610778 70-71 23.02966369064018 26.416597198399593 27.04276697588192 23.510972135078305 72-73 22.905759947494445 26.410081457820144 27.24570009667048 23.43845849801493 74-75 22.6825469016234 26.959010250714478 26.866027958675424 23.492414888986698 76-77 22.865137444874335 26.636584842800648 26.869734364599978 23.62854334772503 78-79 22.793296561052635 26.67607080789557 26.975158492143937 23.55547413890786 80-81 22.87631000051768 26.478079619059216 26.998769345482426 23.646841034940678 82-83 22.914669361857086 26.515950184598175 27.096872884430677 23.47250756911406 84-85 22.754088832415693 26.593946441755428 26.941002569066292 23.71096215676259 86-87 22.627888679185666 26.655128786506644 27.07652916593053 23.64045336837716 88-89 22.66088145288222 26.587257220861314 26.778684330269563 23.973176995986904 90-91 22.726533487680207 26.437000446431806 26.8741134761655 23.96235258972248 92-93 22.73332611989228 26.56741816058586 26.8857353166297 23.81352040289216 94-95 22.7215061487179 26.34581591905719 27.024031201169024 23.908646731055885 96-97 22.393569630490376 26.284152034180362 27.285633983065345 24.036644352263917 98-99 22.42342958241064 26.43015541262475 26.820458859279466 24.325956145685144 100-101 22.538499377451203 25.78446258619479 27.39784600782252 24.279192028531487 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 639.0 1 463.5 2 372.0 3 468.0 4 427.5 5 442.0 6 788.0 7 1522.5 8 2334.5 9 2809.0 10 2760.0 11 2573.5 12 2707.5 13 3022.0 14 3278.5 15 3538.5 16 3827.5 17 4146.5 18 4513.0 19 5048.5 20 5968.5 21 7257.5 22 9320.0 23 12160.0 24 14701.5 25 17830.5 26 22721.5 27 28614.5 28 33621.0 29 38400.0 30 43896.0 31 51348.5 32 59162.5 33 65500.0 34 73408.0 35 81340.5 36 89864.0 37 98081.5 38 104190.0 39 110195.5 40 116533.5 41 122351.5 42 128132.5 43 133208.0 44 138566.5 45 146076.5 46 154718.5 47 169559.5 48 190772.0 49 193195.5 50 177547.5 51 170738.0 52 172570.5 53 173987.5 54 173023.5 55 165166.5 56 151860.5 57 139135.5 58 129186.5 59 116469.0 60 99139.0 61 82132.0 62 65034.0 63 50636.5 64 37342.0 65 25606.5 66 18025.5 67 13631.0 68 10064.5 69 7573.0 70 5796.0 71 3684.5 72 2403.5 73 1697.0 74 1073.0 75 587.5 76 325.0 77 156.5 78 89.5 79 41.0 80 20.5 81 14.0 82 8.5 83 8.5 84 9.0 85 5.5 86 2.0 87 4.0 88 3.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40541168048662735 2 0.0 3 0.0 4 0.0 5 2.8883388208067934E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014886053922619625 16-17 0.002343998043039359 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002388434024897925 36-37 0.008054021711865096 38-39 0.01404177026730687 40-41 0.0024217610112918495 42-43 4.99904795908868E-4 44-45 3.777058457978114E-4 46-47 3.554878548685284E-4 48-49 3.332698639392453E-4 50-51 7.220847052016983E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.4439790022211326E-4 60-61 0.0026772679069786043 62-63 0.0 64-65 1.6663493196962265E-4 66-67 0.0018996382244536986 68-69 0.0019774011927061893 70-71 2.2217990929283024E-5 72-73 0.0 74-75 0.0 76-77 0.1260426625418226 78-79 6.665397278784907E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1483050894529642 88-89 0.43942742459935963 90-91 0.3397019723132728 92-93 0.0 94-95 0.0 96-97 5.998857550906417E-4 98-99 0.0 100-101 1.1108995464641512E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4500857.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.23601513080029 #Duplication Level Percentage of deduplicated Percentage of total 1 68.44098900294082 30.959976141039675 2 15.442617001743189 13.971249127000174 3 6.215371859609195 8.434759664544957 4 3.075380814940323 5.564718923104535 5 1.776500677089675 4.018090575435275 6 1.1457774886050345 3.1098244686640615 7 0.7671072752887673 2.429061341833676 8 0.5514012800074782 1.9954557316448744 9 0.39600263843863187 1.6122223209822109 >10 1.9857492168123496 15.719775853219803 >50 0.1326074920757165 4.114295118765251 >100 0.06715802023235719 5.423122471580274 >500 0.0019140010448226157 0.5823179440042411 >1k 0.0011778464869112906 1.1322229819530596 >5k 1.9630774781850726E-4 0.6335120590786117 >10k+ 4.9076936954626816E-5 0.29939527714936076 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13486 0.29963182567231084 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9100 0.20218371745647554 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8531 0.18954168061771345 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5652 0.12557608473230764 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5253 0.11671110635152374 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4551 0.10111407671916704 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 2.2217990929283024E-5 6 0.0 0.0 0.0 0.0 4.443598185856605E-5 7 0.0 0.0 0.0 0.0 4.443598185856605E-5 8 0.0 0.0 0.0 0.0 6.665397278784907E-5 9 0.0 0.0 0.0 0.0 8.88719637171321E-5 10-11 0.0 0.0 0.0 0.0 8.88719637171321E-5 12-13 0.0 0.0 0.0 0.0 8.88719637171321E-5 14-15 0.0 0.0 0.0 3.3326986393924534E-5 1.4441694104033964E-4 16-17 0.0 0.0 0.0 5.554497732320756E-5 1.777439274342642E-4 18-19 0.0 0.0 0.0 6.665397278784907E-5 1.777439274342642E-4 20-21 0.0 2.2217990929283024E-5 0.0 1.5552593650498115E-4 1.777439274342642E-4 22-23 0.0 2.2217990929283024E-5 0.0 3.221608684746038E-4 1.888529228989057E-4 24-25 0.0 3.3326986393924534E-5 0.0 5.110137913735095E-4 2.2217990929283024E-4 26-27 0.0 4.443598185856605E-5 0.0 8.109566689188303E-4 2.2217990929283024E-4 28-29 0.0 4.443598185856605E-5 0.0 0.0028994478162714344 2.2217990929283024E-4 30-31 0.0 4.443598185856605E-5 0.0 0.009309338199369587 2.2217990929283024E-4 32-33 0.0 4.443598185856605E-5 0.0 0.02091823845991997 2.4439790022211326E-4 34-35 1.1108995464641512E-5 4.443598185856605E-5 0.0 0.03963689581784091 2.4439790022211326E-4 36-37 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.07047546722768575 2.4439790022211326E-4 38-39 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.11792198685716965 2.6661589115139627E-4 40-41 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.167523651606794 2.999428775453208E-4 42-43 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.21891386462622564 3.3326986393924536E-4 44-45 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.28461246380411553 3.554878548685284E-4 46-47 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.3694074261857242 3.554878548685284E-4 48-49 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.45803499200263414 3.777058457978114E-4 50-51 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.5547054705359447 3.777058457978114E-4 52-53 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.6543309418628497 4.33250823121019E-4 54-55 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.7673427527246478 4.887958004442265E-4 56-57 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.906782863796828 4.887958004442265E-4 58-59 2.2217990929283024E-5 4.443598185856605E-5 0.0 1.0578874201068817 4.887958004442265E-4 60-61 2.2217990929283024E-5 4.443598185856605E-5 0.0 1.221411833346405 4.887958004442265E-4 62-63 2.2217990929283024E-5 4.443598185856605E-5 0.0 1.3909351041368345 5.110137913735095E-4 64-65 2.2217990929283024E-5 4.443598185856605E-5 0.0 1.5706564327638048 5.332317823027925E-4 66-67 2.2217990929283024E-5 6.665397278784907E-5 0.0 1.7817273465919934 5.554497732320756E-4 68-69 2.2217990929283024E-5 6.665397278784907E-5 0.0 2.0236257228345624 5.554497732320756E-4 70-71 2.2217990929283024E-5 6.665397278784907E-5 0.0 2.2899083441220194 5.665587686967171E-4 72-73 2.2217990929283024E-5 6.665397278784907E-5 0.0 2.561156686382171 6.221037460199247E-4 74-75 2.2217990929283024E-5 6.665397278784907E-5 0.0 2.847935404301892 6.221037460199247E-4 76-77 2.2217990929283024E-5 6.665397278784907E-5 0.0 3.1608646975453785 6.221037460199247E-4 78-79 2.2217990929283024E-5 6.665397278784907E-5 0.0 3.522340300969349 6.221037460199247E-4 80-81 2.2217990929283024E-5 6.665397278784907E-5 0.0 3.919786831707828 6.332127414845662E-4 82-83 2.2217990929283024E-5 6.665397278784907E-5 0.0 4.320354990171872 6.887577188077737E-4 84-85 2.2217990929283024E-5 6.665397278784907E-5 0.0 4.729521511125547 6.887577188077737E-4 86-87 2.2217990929283024E-5 6.665397278784907E-5 0.0 5.163838797811172 6.887577188077737E-4 88-89 2.2217990929283024E-5 6.665397278784907E-5 2.2217990929283024E-5 5.646757939654604 6.887577188077737E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 11340 0.0 22.30234 2 GTACATG 11625 0.0 22.213491 1 ACATGGG 11495 0.0 20.804525 3 AGAGTAC 14995 0.0 18.877674 10-11 GAGTACA 9330 0.0 18.741093 1 GAGTACT 10785 0.0 18.480701 12-13 AGTACAT 8480 0.0 17.961617 2 GTACTTT 11190 0.0 17.620989 14-15 AGTACTT 11415 0.0 17.377596 12-13 CCTATAC 1140 0.0 17.065351 3 CATGGGG 7610 0.0 16.64803 4 GTATCAA 25130 0.0 15.887735 1 GTGTAGC 2495 0.0 15.849591 1 TAGACAG 2240 0.0 15.463617 5 GGTATCA 21985 0.0 15.061522 1 TACTTTT 13540 0.0 14.562693 14-15 TCTAGAC 2870 0.0 14.3838 3 CAACGCA 27505 0.0 14.301419 5 ATCAACG 27720 0.0 14.207614 3 GTATAGG 1210 0.0 14.175149 1 >>END_MODULE