FastQCFastQC Report
Fri 27 May 2016
SRR939011_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939011_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4430238
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT93850.21183963480065857No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85620.19326275473236426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67560.15249745047557264No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49280.1112355589022531No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG120200.021.7597221
TACATGG114450.021.5630132
GTATCAA224950.020.5320841
ACATGGG115400.020.3182813
GAGTACT97950.020.25388112-13
GGTATCA179750.018.5384921
AGAGTAC154950.018.3636710-11
GTACTTT107150.018.1841714-15
TATCAAC252050.018.116652
TCAACGC251600.018.0172654
ATCAACG251950.017.9734343
CAACGCA252000.017.9320745
AACGCAG255900.017.6878076
AGTACTT103350.017.45473112-13
GGACCGA12700.017.1490676
CATGGGG81200.016.6255654
GAGTACA97400.016.3751961
ACGCAGA277000.016.0328037
GTGTAGC26950.016.028351
AGTACAT90800.015.6503112
Produced by FastQC (version 0.11.5)