##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939011_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4430238 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.735399091425787 33.0 31.0 34.0 30.0 34.0 2 31.829667616051328 33.0 31.0 34.0 30.0 34.0 3 31.886802695475954 34.0 31.0 34.0 30.0 34.0 4 35.409442336957966 37.0 35.0 37.0 33.0 37.0 5 35.44335022181652 37.0 35.0 37.0 33.0 37.0 6 35.51785434552274 37.0 35.0 37.0 33.0 37.0 7 35.431896209639305 37.0 35.0 37.0 33.0 37.0 8 35.415419216755396 37.0 35.0 37.0 33.0 37.0 9 37.01952784477945 39.0 37.0 39.0 33.0 39.0 10-11 36.898341578940006 39.0 37.0 39.0 33.0 39.0 12-13 36.784442957692114 39.0 37.0 39.0 32.0 39.0 14-15 37.99866214862497 40.0 38.0 41.0 33.0 41.0 16-17 37.90246641376829 40.0 38.0 41.0 33.0 41.0 18-19 37.788739566587616 40.0 38.0 41.0 32.0 41.0 20-21 37.89771204165555 40.0 38.0 41.0 32.0 41.0 22-23 37.833311212625595 40.0 38.0 41.0 32.0 41.0 24-25 37.75532646327353 40.0 38.0 41.0 32.0 41.0 26-27 37.63552285452836 40.0 37.5 41.0 32.0 41.0 28-29 37.504732251405 40.0 37.0 41.0 32.0 41.0 30-31 37.327907665457246 40.0 37.0 41.0 31.0 41.0 32-33 37.140919855772985 40.0 37.0 41.0 30.5 41.0 34-35 36.82487261406723 40.0 36.0 41.0 30.0 41.0 36-37 36.56663287615699 39.5 36.0 41.0 30.0 41.0 38-39 36.33093492945525 39.0 35.5 41.0 30.0 41.0 40-41 36.19380753359074 39.0 35.0 41.0 29.5 41.0 42-43 35.99571580578741 39.0 35.0 41.0 28.5 41.0 44-45 35.9724647976023 39.0 35.0 41.0 28.0 41.0 46-47 35.813647709220135 39.0 35.0 41.0 27.5 41.0 48-49 35.60894629137306 39.0 35.0 41.0 27.0 41.0 50-51 34.52088318957131 38.0 34.0 40.0 25.0 40.5 52-53 34.62778805563042 38.0 34.0 39.5 25.5 40.5 54-55 35.11982347223783 39.0 35.0 40.0 26.0 41.0 56-57 35.01170625596187 38.5 34.5 40.0 26.0 41.0 58-59 34.793121611073715 38.0 34.0 40.0 26.0 41.0 60-61 34.47300054760038 38.0 34.0 40.0 24.5 41.0 62-63 34.08832708310479 37.5 34.0 40.0 23.5 41.0 64-65 33.70140035366046 37.0 33.0 40.0 22.5 41.0 66-67 33.27761612355815 36.0 33.0 39.0 22.0 41.0 68-69 32.82779469184274 36.0 32.0 39.0 20.5 40.5 70-71 32.38075279477084 35.0 32.0 38.5 19.5 40.0 72-73 31.940739301139125 35.0 31.5 37.5 18.0 40.0 74-75 31.466369414013425 35.0 31.0 37.0 16.0 39.0 76-77 31.028570022648896 35.0 31.0 36.5 12.0 39.0 78-79 30.621746619481844 35.0 30.5 36.0 7.5 39.0 80-81 30.16281247192589 34.0 30.0 36.0 6.5 37.5 82-83 29.68756181947787 34.0 29.5 35.0 2.0 37.0 84-85 29.264954614176485 34.0 29.0 35.0 2.0 36.5 86-87 28.818573516817832 34.0 29.0 35.0 2.0 36.0 88-89 28.331632183191964 34.0 28.5 35.0 2.0 36.0 90-91 27.865984287977305 34.0 27.0 35.0 2.0 35.0 92-93 27.34618095009794 33.5 26.0 35.0 2.0 35.0 94-95 26.794374816883426 33.0 25.0 35.0 2.0 35.0 96-97 26.120818339782197 33.0 24.5 35.0 2.0 35.0 98-99 25.22355853116695 32.0 20.0 35.0 2.0 35.0 100-101 21.93945043133123 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19135405496648872 1101 2 0.31949668637354023 1101 3 0.33111315531519736 1101 4 0.30108699921631654 1101 5 0.08477892742461535 1101 6 0.2686541628254062 1101 7 0.28360387937397036 1101 8 0.26436950599730125 1101 9 0.33839026553208384 1101 10-11 0.25285516493050864 1101 12-13 0.289233397674586 1101 14-15 0.46434967926525417 1101 16-17 0.47254083714037876 1101 18-19 0.6702945390658215 1101 20-21 0.4696025348585735 1101 22-23 0.5323861068764018 1101 24-25 0.6216965171676989 1101 26-27 0.5816274557190084 1101 28-29 0.5404645555359124 1101 30-31 0.6100477227437509 1101 32-33 0.5722820697353157 1101 34-35 0.43638861871204426 1101 36-37 0.47760745869659615 1101 38-39 0.6004644062202473 1101 40-41 0.5190376405523338 1101 42-43 0.5140390374022061 1101 44-45 0.6004955900717732 1101 46-47 0.6015667331025156 1101 48-49 0.6461483491332771 1101 50-51 0.5525092264493097 1101 52-53 0.47957908782509406 1101 54-55 0.6873757662777109 1101 56-57 0.6693018353986346 1101 58-59 0.6320851122215316 1101 60-61 0.5962302120845777 1101 62-63 0.6145395092764261 1101 64-65 0.507907309335387 1101 66-67 0.5447577574213582 1101 68-69 0.5070954232188214 1101 70-71 0.4209426859901626 1101 72-73 0.3157029357106502 1101 74-75 0.20279218908676455 1101 76-77 0.21476264071865714 1101 78-79 0.31876633924325404 1101 80-81 0.2938859859558498 1101 82-83 0.37367691385608026 1101 84-85 0.4727101703183294 1101 86-87 0.42223410708282927 1101 88-89 0.1937027464547043 1101 90-91 0.2711404009574778 1101 92-93 0.23926766296981583 1101 94-95 0.06865854614258637 1101 96-97 -0.03083767825877004 1101 98-99 -0.1255929888715741 1101 100-101 0.3378070472954633 1102 1 0.3136914517376397 1102 2 0.30497126806784536 1102 3 0.35390563695063193 1102 4 0.1543397308809915 1102 5 0.3383971636017833 1102 6 0.3293474718640397 1102 7 0.3940897479543537 1102 8 0.3830030768535764 1102 9 0.46516617813787775 1102 10-11 0.479759247721816 1102 12-13 0.482452855956474 1102 14-15 0.5629737750933543 1102 16-17 0.6086153868144706 1102 18-19 0.5317972697179556 1102 20-21 0.5365066986533904 1102 22-23 0.5518083064600035 1102 24-25 0.5847735391165472 1102 26-27 0.69191680144651 1102 28-29 0.6152770145427766 1102 30-31 0.6555257435135289 1102 32-33 0.6340894748379142 1102 34-35 0.6139724638962321 1102 36-37 0.5236658457880594 1102 38-39 0.6669218297034973 1102 40-41 0.6334952720240068 1102 42-43 0.5239614908365056 1102 44-45 0.7340604604220786 1102 46-47 0.7195674640400611 1102 48-49 0.6960739390711126 1102 50-51 0.6440482252316357 1102 52-53 0.5964794218216198 1102 54-55 0.7581974325246748 1102 56-57 0.6771730834173226 1102 58-59 0.5685201989870095 1102 60-61 0.7642671980488132 1102 62-63 0.7519008235250197 1102 64-65 0.6575854752443959 1102 66-67 0.6693444608382109 1102 68-69 0.639544351101641 1102 70-71 0.564271427536319 1102 72-73 0.5569631632971586 1102 74-75 0.530904111635973 1102 76-77 0.6168967020035332 1102 78-79 0.5739680153488109 1102 80-81 0.5493463372394807 1102 82-83 0.5927909903839037 1102 84-85 0.6209580286689196 1102 86-87 0.47087915661290225 1102 88-89 0.328356935324873 1102 90-91 0.3728565377380981 1102 92-93 0.40395607701401204 1102 94-95 0.27191079535989005 1102 96-97 0.26816345912580175 1102 98-99 0.13583401541136553 1102 100-101 0.2668242972310786 1103 1 0.32464259764743275 1103 2 0.2921167329935912 1103 3 0.4556776709683241 1103 4 0.4171257115588247 1103 5 0.23354497150711495 1103 6 0.38239834269616324 1103 7 0.2514601834074881 1103 8 0.30266628095230885 1103 9 0.40612748190221737 1103 10-11 0.47440126861584986 1103 12-13 0.45922826356818547 1103 14-15 0.5922134189011388 1103 16-17 0.593391357927608 1103 18-19 0.6344232753084853 1103 20-21 0.6566739992591479 1103 22-23 0.6117115325056304 1103 24-25 0.7259490030784121 1103 26-27 0.6850814995654488 1103 28-29 0.5687255097715536 1103 30-31 0.6014443472163293 1103 32-33 0.5669162203947806 1103 34-35 0.5465491037965862 1103 36-37 0.5398113181283009 1103 38-39 0.7021164082266793 1103 40-41 0.6255149649157374 1103 42-43 0.48740813244219083 1103 44-45 0.6349376838429777 1103 46-47 0.7407404960484243 1103 48-49 0.6318940338283241 1103 50-51 0.5673890175442864 1103 52-53 0.595058241582727 1103 54-55 0.6356110499813497 1103 56-57 0.6011126701335172 1103 58-59 0.5652098060930655 1103 60-61 0.6248761947938419 1103 62-63 0.6256018267058323 1103 64-65 0.5716072886809442 1103 66-67 0.6775524521780838 1103 68-69 0.6316216101120986 1103 70-71 0.6292100726119827 1103 72-73 0.6575728421755009 1103 74-75 0.5853043014469783 1103 76-77 0.6058232762856228 1103 78-79 0.6210444661796046 1103 80-81 0.5686608229332144 1103 82-83 0.7227351889143563 1103 84-85 0.579918101627527 1103 86-87 0.722184359362199 1103 88-89 0.7099262571212535 1103 90-91 0.7626539886356234 1103 92-93 0.6375489175801867 1103 94-95 0.5078993770416425 1103 96-97 0.36381975291764945 1103 98-99 0.3163731083223702 1103 100-101 0.506636028440898 1104 1 0.2117841326721681 1104 2 0.2749501606159086 1104 3 0.14964445223237632 1104 4 0.26187851484298363 1104 5 0.26370207146795366 1104 6 0.23781520156730807 1104 7 0.2214084550178015 1104 8 0.22499228875653188 1104 9 0.19786596776611987 1104 10-11 0.29417717995519865 1104 12-13 0.32674103609962657 1104 14-15 0.3581372333984376 1104 16-17 0.34980533010510584 1104 18-19 0.40979377300928377 1104 20-21 0.35396720900137524 1104 22-23 0.3847249215420021 1104 24-25 0.4002621875802035 1104 26-27 0.301347771668965 1104 28-29 0.33705102437183854 1104 30-31 0.38664045380640033 1104 32-33 0.4086815713674028 1104 34-35 0.40229321798779694 1104 36-37 0.42751121803691206 1104 38-39 0.38739476382671967 1104 40-41 0.4341578417453036 1104 42-43 0.383711075709229 1104 44-45 0.5557535882194387 1104 46-47 0.5965252560466823 1104 48-49 0.5157463337543433 1104 50-51 0.469040351370829 1104 52-53 0.5322584678762965 1104 54-55 0.6393079906663246 1104 56-57 0.6939389898167079 1104 58-59 0.5988473592212813 1104 60-61 0.6529422260977924 1104 62-63 0.6512830138767285 1104 64-65 0.7290779938175191 1104 66-67 0.6764907581676098 1104 68-69 0.5914509615489152 1104 70-71 0.5954802903470195 1104 72-73 0.6013016670445772 1104 74-75 0.5773785721579152 1104 76-77 0.6557456401830954 1104 78-79 0.5405556720632418 1104 80-81 0.5970881116170865 1104 82-83 0.7722573903446488 1104 84-85 0.7703095024717754 1104 86-87 0.6774101742366483 1104 88-89 0.6774416407323756 1104 90-91 0.6846445887487711 1104 92-93 0.6756953411263211 1104 94-95 0.7982405291080248 1104 96-97 0.7278046956914856 1104 98-99 0.8860535992053507 1104 100-101 1.0943943294983889 1105 1 0.19810370745043215 1105 2 0.22317982922551494 1105 3 0.2811703467251867 1105 4 0.2554311963573639 1105 5 0.23350301675831275 1105 6 0.28563596497499333 1105 7 0.3307043700347734 1105 8 0.2699431751592698 1105 9 0.38999522455121394 1105 10-11 0.37152020462059454 1105 12-13 0.3092517667897141 1105 14-15 0.4383295365171449 1105 16-17 0.41728615710849226 1105 18-19 0.39498344255666495 1105 20-21 0.5124923380508832 1105 22-23 0.30246901550610517 1105 24-25 0.4297031254178876 1105 26-27 0.32420647191670326 1105 28-29 0.3404081092535378 1105 30-31 0.45515692864635326 1105 32-33 0.44835869315630106 1105 34-35 0.44389936173266875 1105 36-37 0.49043503112356746 1105 38-39 0.5491509067318603 1105 40-41 0.6478335498705334 1105 42-43 0.5054517282781106 1105 44-45 0.6604789072377528 1105 46-47 0.6428520186205091 1105 48-49 0.663730752795729 1105 50-51 0.6556782484452484 1105 52-53 0.7235971460500465 1105 54-55 0.8667259760253998 1105 56-57 0.7451413534821896 1105 58-59 0.604589874733314 1105 60-61 0.6601561713905184 1105 62-63 0.683243456091283 1105 64-65 0.8084374504350436 1105 66-67 0.6552185408966764 1105 68-69 0.7260140011760186 1105 70-71 0.5866556848445086 1105 72-73 0.6249268532064569 1105 74-75 0.6017226787685779 1105 76-77 0.6193166084773303 1105 78-79 0.7043910851833957 1105 80-81 0.6409686623350055 1105 82-83 0.6974665572242493 1105 84-85 0.8014175985986505 1105 86-87 0.8579600298889822 1105 88-89 0.8558608847728557 1105 90-91 0.9142809338589863 1105 92-93 0.8615687247881247 1105 94-95 0.7886437456155413 1105 96-97 0.7145909925092297 1105 98-99 0.6760221440053122 1105 100-101 0.50106854765232 1106 1 0.1868843220508225 1106 2 0.20600405890863271 1106 3 0.23238954723678162 1106 4 0.23084451623375202 1106 5 0.189404723764099 1106 6 0.23233073824518868 1106 7 0.23823518515708741 1106 8 0.19461365305672018 1106 9 0.239087940996626 1106 10-11 0.20290739220742182 1106 12-13 0.24024275023625563 1106 14-15 0.34395461842525776 1106 16-17 0.392217028023488 1106 18-19 0.4819354874996762 1106 20-21 0.3819132742259086 1106 22-23 0.5180505345351634 1106 24-25 0.3971307512763289 1106 26-27 0.30633253182803344 1106 28-29 0.2993352095406081 1106 30-31 0.34750131411393426 1106 32-33 0.3093520801470362 1106 34-35 0.28778739898869077 1106 36-37 0.24980124044616758 1106 38-39 0.3873251702114189 1106 40-41 0.4725237129036941 1106 42-43 0.28294609870994947 1106 44-45 0.25935100877824624 1106 46-47 0.25312050864552305 1106 48-49 0.3630769017687001 1106 50-51 0.14507367007420413 1106 52-53 0.213277431169125 1106 54-55 0.344249303183112 1106 56-57 0.311942399295809 1106 58-59 0.2349371488042351 1106 60-61 0.45940177391064907 1106 62-63 0.5084101407935933 1106 64-65 0.2985914023348002 1106 66-67 0.3985105318197668 1106 68-69 0.4875444878502222 1106 70-71 0.36689920411173915 1106 72-73 0.34063659751176445 1106 74-75 0.43489335596443723 1106 76-77 0.253062831187993 1106 78-79 0.37527625286866595 1106 80-81 0.3642780109799695 1106 82-83 0.4398787259008472 1106 84-85 0.5076397056586011 1106 86-87 0.5684363014155238 1106 88-89 0.6107748819375445 1106 90-91 0.6830894284844575 1106 92-93 0.5867755954442764 1106 94-95 0.6150556510986114 1106 96-97 0.7369186401735774 1106 98-99 0.7494165683265699 1106 100-101 0.8331017013361368 1107 1 0.1669560511841901 1107 2 0.1683800037034615 1107 3 0.23807299732481013 1107 4 0.21966158345392017 1107 5 0.12736500260870542 1107 6 0.15144839943554445 1107 7 0.11104644827531018 1107 8 0.17925317625764592 1107 9 0.19335330462318012 1107 10-11 0.24417758938246692 1107 12-13 0.34766780460966373 1107 14-15 0.435964101875129 1107 16-17 0.43124240655535573 1107 18-19 0.4270901941501606 1107 20-21 0.41757123794279494 1107 22-23 0.35220184478234984 1107 24-25 0.3905979334360765 1107 26-27 0.4121807304316718 1107 28-29 0.4378859351067348 1107 30-31 0.47263528572765523 1107 32-33 0.4265321578553696 1107 34-35 0.3940293711020928 1107 36-37 0.3625891099158025 1107 38-39 0.5035002606202426 1107 40-41 0.3563242785794216 1107 42-43 0.493134774731935 1107 44-45 0.3247899092874391 1107 46-47 0.5360532350893195 1107 48-49 0.6446567791735944 1107 50-51 0.48658298215572415 1107 52-53 0.5318298971116633 1107 54-55 0.6392206830667106 1107 56-57 0.59152169799885 1107 58-59 0.7025194349755068 1107 60-61 0.6438411004383653 1107 62-63 0.5331681697479027 1107 64-65 0.5418091127468401 1107 66-67 0.4788108054752698 1107 68-69 0.6428637401503465 1107 70-71 0.5653900415103976 1107 72-73 0.6630692486816123 1107 74-75 0.7206733595733219 1107 76-77 0.7517452701493639 1107 78-79 0.7180022268655222 1107 80-81 0.8367223536355262 1107 82-83 0.6938429937308968 1107 84-85 0.7676400366504303 1107 86-87 0.8715319826127335 1107 88-89 0.9258284972759192 1107 90-91 0.9753583134370345 1107 92-93 0.8873723950735659 1107 94-95 0.7578540695982774 1107 96-97 0.8525105443268863 1107 98-99 0.8268697249743262 1107 100-101 0.9412372136634453 1108 1 0.3589345345519135 1108 2 0.28526194441600694 1108 3 0.3442057283861182 1108 4 0.3491474033749 1108 5 0.3062014910625095 1108 6 0.30644804461567077 1108 7 0.39080031545096006 1108 8 0.367442527685796 1108 9 0.4192691702243039 1108 10-11 0.46397656032711154 1108 12-13 0.49047522271041544 1108 14-15 0.6175563024568547 1108 16-17 0.6525504724938784 1108 18-19 0.6464176267013428 1108 20-21 0.47798645603929657 1108 22-23 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-2.2922679731517768 2316 72-73 -2.4184667372926576 2316 74-75 -2.3274143132716354 2316 76-77 -2.242959655068738 2316 78-79 -2.2029481126701143 2316 80-81 -2.1068666971657315 2316 82-83 -2.022662461054722 2316 84-85 -2.121076332509766 2316 86-87 -2.119756271435577 2316 88-89 -2.25792903044832 2316 90-91 -2.4065445271343187 2316 92-93 -2.4175222017766487 2316 94-95 -2.4923059414493878 2316 96-97 -2.7444177238912566 2316 98-99 -2.8132038861117685 2316 100-101 -2.6039984799078972 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13224.0 3 4000.0 4 4378.0 5 4820.0 6 5419.0 7 6842.0 8 8445.0 9 10242.0 10 12796.0 11 16003.0 12 19929.0 13 26971.0 14 23420.0 15 22925.0 16 27195.0 17 34130.0 18 31041.0 19 26161.0 20 27384.0 21 29674.0 22 32704.0 23 37410.0 24 41989.0 25 48216.0 26 56031.0 27 65627.0 28 76814.0 29 91105.0 30 109339.0 31 132384.0 32 163445.0 33 208826.0 34 279833.0 35 393731.0 36 597906.0 37 885933.0 38 747133.0 39 106787.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14434075278484 18.880537696988362 13.935872206382887 24.039249343843906 2 15.322087091914089 20.197486595848073 40.09525063894649 24.38517567329135 3 19.60166660980558 23.66466628603628 29.57597518838154 27.157691915776606 4 12.934602929573288 16.265259512255962 37.26992672903481 33.53021082913594 5 13.986249915824573 36.939047048301106 34.57933302329659 14.49537001257773 6 32.45431336572886 35.74314576269961 17.418783080971647 14.383757790599876 7 28.53156566369483 32.06836545139379 21.111531447091306 18.288537437820075 8 25.65369087014537 35.216124751221194 19.95608032111099 19.17410405752245 9 25.667352381571884 17.102353113362497 20.738720034981398 36.49157447008422 10-11 24.907500970048428 26.131455903789263 27.65975082742082 21.30129229874149 12-13 25.8003077012028 23.477484330365936 27.842758000140932 22.879449968290334 14-15 22.710973228857902 25.033337876627105 26.06826029753342 26.187428596981576 16-17 22.424297928985933 28.03614574294564 26.71906950944579 22.82048681862264 18-19 22.342116188102196 27.351819535802324 28.21475342519591 22.09131085089957 20-21 23.070537825793323 26.422840712222207 28.157239111235594 22.349382350748883 22-23 22.764845307352125 26.26177431626735 28.056205417089114 22.91717495929141 24-25 22.74297675542989 26.335746617580114 28.20697009508167 22.71430653190833 26-27 22.546640729954067 26.481788706911946 28.36153768212677 22.610032881007218 28-29 22.445601307208737 26.94738837252015 28.113967800067368 22.493042520203744 30-31 22.6522197555448 26.55614998026219 28.020475367832056 22.771154896360958 32-33 22.54550851012358 26.58947460199465 28.056934738213553 22.808082149668216 34-35 22.92084187623671 26.572766061903835 28.018243429607153 22.488148632252297 36-37 22.845809676290553 26.302979156847968 28.163317390882685 22.687893775978793 38-39 22.551873732187115 26.78404406454914 27.978872658061565 22.685209545202177 40-41 22.873868041438158 26.665876785311383 27.886118761118333 22.574136412132123 42-43 22.537951089169322 26.685776890125858 27.768164068923827 23.008107951780993 44-45 22.73505822657431 26.597596984837647 27.763285391822407 22.904059396765636 46-47 22.929367407413963 26.80213140503807 27.474373949957737 22.794127237590235 48-49 22.753476804627162 26.731346497082047 27.626618442089327 22.88855825620146 50-51 22.53311195739727 27.00104687359804 27.66101168893528 22.804829480069415 52-53 22.875417829162263 27.061218693156064 27.202465027580015 22.860898450101658 54-55 22.772328885611223 26.82731714465473 27.3752738167086 23.025080153025442 56-57 22.706850440989427 26.881077099213098 27.442270392472423 22.969802067325052 58-59 22.931195268835005 26.75336160679107 27.38836358812515 22.927079536248776 60-61 22.77985342314507 26.842994396550846 27.63960626028646 22.737545920017627 62-63 22.584828991272555 26.80209046769911 27.72466851461271 22.888412026415626 64-65 22.422268785305878 27.044571806616265 27.56794706827837 22.96521233979949 66-67 22.588503998682157 26.8628145476427 27.26289831961412 23.28578313406102 68-69 22.49281034212401 26.763125593275838 27.601580510665976 23.142483553934177 70-71 22.780488552060767 26.55701787976416 27.398093513486838 23.264400054688238 72-73 22.672502262713454 26.484388808706612 27.563831889636308 23.27927703894363 74-75 22.351291747183065 27.0892239873653 27.348173574390344 23.21131069106129 76-77 22.504873412631934 26.655352553563873 27.347208717258365 23.49256531654583 78-79 22.62534640629901 26.663640419205205 27.440969463628445 23.270043710867345 80-81 22.71841000354849 26.607008004576183 27.32921508702083 23.345366904854494 82-83 22.59718919939046 26.4976978908788 27.649875265562546 23.255237644168194 84-85 22.627170470083648 26.554488473609574 27.43169319576853 23.38664786053825 86-87 22.425632223356136 26.58302803497493 27.567585468985236 23.4237542726837 88-89 22.573565290535175 26.556776681418633 27.281194911242096 23.588463116804103 90-91 22.571589752909244 26.330887688773437 27.433021567252165 23.664500991065157 92-93 22.6447071369417 26.34549105471542 27.47647679444564 23.53332501389724 94-95 22.60071937263028 26.271260186413336 27.479725879159783 23.6482945617966 96-97 22.42187507090303 26.140914837406303 27.75847954861656 23.6787305430741 98-99 22.477857994611426 26.25943309160129 27.39128496294946 23.871423950837823 100-101 22.803871139706402 25.46926368382101 27.905972024125152 23.820893152347438 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2781.0 1 1557.0 2 376.0 3 421.0 4 404.5 5 478.0 6 757.0 7 1359.0 8 2138.5 9 2642.0 10 2735.5 11 2872.5 12 3213.5 13 3577.0 14 4049.0 15 4557.0 16 4950.0 17 5259.0 18 5543.5 19 5890.0 20 6347.5 21 7149.5 22 8434.0 23 10531.5 24 13149.5 25 16519.5 26 22145.0 27 28982.5 28 34593.5 29 40211.0 30 46822.0 31 54484.5 32 62472.5 33 69843.5 34 78557.0 35 87961.5 36 98132.5 37 107716.5 38 115081.5 39 119994.0 40 124884.5 41 131196.5 42 137258.0 43 144233.0 44 152281.0 45 158042.0 46 161846.0 47 171524.0 48 186004.5 49 184996.0 50 172205.0 51 165517.5 52 161917.0 53 158482.5 54 153558.5 55 144484.5 56 133311.5 57 121501.5 58 111666.5 59 101760.5 60 85790.5 61 69248.0 62 55171.5 63 42420.5 64 32594.0 65 24936.0 66 17880.0 67 12757.5 68 9192.0 69 6513.5 70 4604.5 71 3078.5 72 2030.0 73 1349.5 74 900.0 75 560.5 76 353.5 77 210.0 78 134.5 79 106.5 80 80.5 81 57.5 82 49.0 83 41.0 84 34.0 85 33.0 86 28.5 87 19.5 88 18.5 89 16.5 90 10.0 91 7.0 92 6.5 93 5.5 94 6.5 95 7.5 96 3.5 97 4.0 98 4.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3243166619942314 2 0.08602246651308576 3 0.095863924240639 4 0.10098780246117704 5 0.11186757912328865 6 0.055662923752629094 7 0.05785242237550217 8 0.05891331346081181 9 0.06189283735997931 10-11 0.05922932357132958 12-13 0.057920138827755985 14-15 0.05567420982800472 16-17 0.05760412871723821 18-19 0.0654592371786798 20-21 0.06699414342976608 22-23 0.08247863884513654 24-25 0.09125920548738015 26-27 0.08628204624672535 28-29 0.08367496283495379 30-31 0.07799806692100966 32-33 0.06995109517818231 34-35 0.32260117853713505 36-37 0.5010001719997886 38-39 0.4755952163292356 40-41 0.45805665519549965 42-43 0.4876261726796619 44-45 0.49623744819127097 46-47 0.4917230180410172 48-49 0.5084828399738344 50-51 0.5045214275169867 52-53 0.504171559180342 54-55 0.49549256721647916 56-57 0.4754146391232254 58-59 0.45890311084867225 60-61 0.4173816395417131 62-63 0.48664428412198174 64-65 0.5100741766017989 66-67 0.5199720647062301 68-69 0.5318224438506464 70-71 0.5291024996851185 72-73 0.5173311230683317 74-75 0.5193739027113217 76-77 0.4938673723623877 78-79 0.4748503353544437 80-81 0.481294684393931 82-83 0.48918365108149947 84-85 0.4908539902370933 86-87 0.49001882065929636 88-89 0.4968356101861796 90-91 0.5102773259585602 92-93 0.5166313863950425 94-95 0.5237528999570678 96-97 0.5148707586364434 98-99 0.5179744293647429 100-101 0.49603429883450956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4430238.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.34606484936978 #Duplication Level Percentage of deduplicated Percentage of total 1 69.9992239723323 33.841870215719375 2 14.799093174818442 14.30955836683276 3 5.822672844953992 8.445099568764304 4 2.9840458449095952 5.7706749572596685 5 1.7338018642626483 4.19112486828001 6 1.0755967752466105 3.120052286870739 7 0.7200211542682461 2.4367132592019494 8 0.5251102134605141 2.0309609946422733 9 0.4002645171229144 1.741609287155349 >10 1.8016307381721528 14.873204843428567 >50 0.0913773087972898 3.043567095236285 >100 0.043606140124786266 3.8035731522965333 >500 0.00224554870385567 0.706612251274471 >1k 0.0011695560951588417 1.1266618648796491 >5k 1.4034673141902692E-4 0.5587169881583176 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9385 0.21183963480065857 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8562 0.19326275473236426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6756 0.15249745047557264 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4928 0.1112355589022531 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2572150751268893E-5 2 0.0 0.0 0.0 0.0 2.2572150751268893E-5 3 0.0 0.0 0.0 0.0 2.2572150751268893E-5 4 0.0 0.0 0.0 0.0 2.2572150751268893E-5 5 0.0 0.0 0.0 0.0 2.2572150751268893E-5 6 2.2572150751268893E-5 0.0 0.0 0.0 2.2572150751268893E-5 7 1.1286075375634446E-4 0.0 0.0 0.0 2.2572150751268893E-5 8 1.1286075375634446E-4 0.0 0.0 0.0 2.2572150751268893E-5 9 1.1286075375634446E-4 0.0 0.0 2.2572150751268893E-5 2.2572150751268893E-5 10-11 1.1286075375634446E-4 1.1286075375634447E-5 0.0 2.2572150751268893E-5 2.2572150751268893E-5 12-13 1.3543290450761337E-4 2.2572150751268893E-5 0.0 6.771645225380668E-5 6.771645225380668E-5 14-15 2.0314935676142004E-4 2.2572150751268893E-5 0.0 9.028860300507557E-5 7.900252762944113E-5 16-17 2.0314935676142004E-4 2.2572150751268893E-5 0.0 1.3543290450761337E-4 9.028860300507557E-5 18-19 2.595797336395923E-4 2.2572150751268893E-5 0.0 1.5800505525888226E-4 9.028860300507557E-5 20-21 3.160101105177645E-4 2.2572150751268893E-5 0.0 2.2572150751268895E-4 1.1286075375634446E-4 22-23 3.611544120203023E-4 2.2572150751268893E-5 0.0 3.7244048739593674E-4 1.1286075375634446E-4 24-25 3.837265627715712E-4 2.2572150751268893E-5 0.0 6.433062964111635E-4 1.1286075375634446E-4 26-27 3.837265627715712E-4 2.2572150751268893E-5 0.0 0.0010721771606852725 1.1286075375634446E-4 28-29 5.868759195329912E-4 2.2572150751268893E-5 0.0 0.0034309669141928717 1.1286075375634446E-4 30-31 6.771645225380668E-4 2.2572150751268893E-5 0.0 0.009401300787903494 1.1286075375634446E-4 32-33 6.884505979137013E-4 2.2572150751268893E-5 0.0 0.020314935676142003 1.1286075375634446E-4 34-35 7.223088240406046E-4 2.2572150751268893E-5 0.0 0.039331972684086045 1.1286075375634446E-4 36-37 7.448809747918735E-4 2.2572150751268893E-5 0.0 0.06953351038928382 1.1286075375634446E-4 38-39 7.900252762944113E-4 2.2572150751268893E-5 0.0 0.11514054098222262 1.1286075375634446E-4 40-41 7.900252762944113E-4 2.2572150751268893E-5 0.0 0.166503470016735 1.1286075375634446E-4 42-43 8.125974270456801E-4 2.2572150751268893E-5 0.0 0.22306927979941482 1.1286075375634446E-4 44-45 9.028860300507557E-4 2.2572150751268893E-5 0.0 0.29579674952000323 1.3543290450761337E-4 46-47 0.001060891085309638 2.2572150751268893E-5 0.0 0.3816612109778301 1.5800505525888226E-4 48-49 0.0011060353868121758 2.2572150751268893E-5 0.0 0.4738910189475148 1.5800505525888226E-4 50-51 0.0011060353868121758 2.2572150751268893E-5 0.0 0.5742806594137833 1.5800505525888226E-4 52-53 0.0011060353868121758 2.2572150751268893E-5 0.0 0.6803697679447469 2.0314935676142004E-4 54-55 0.0011286075375634447 2.2572150751268893E-5 0.0 0.8023948149061066 2.0314935676142004E-4 56-57 0.0011511796883147136 2.2572150751268893E-5 0.0 0.9495200935028774 2.1443543213705448E-4 58-59 0.0011511796883147136 2.2572150751268893E-5 0.0 1.108529609470191 2.2572150751268893E-4 60-61 0.001185037914441617 2.2572150751268893E-5 0.0 1.282504461385596 2.370075828883234E-4 62-63 0.0011963239898172514 2.2572150751268893E-5 0.0 1.463612112938402 2.7086580901522673E-4 64-65 0.0011963239898172514 2.2572150751268893E-5 0.0 1.6636803711222736 2.821518843908612E-4 66-67 0.0012414682913197892 2.2572150751268893E-5 0.0 1.89806958452345 2.934379597664956E-4 68-69 0.001264040442071058 2.2572150751268893E-5 0.0 2.1644661076899254 3.160101105177645E-4 70-71 0.0012978986681979614 2.2572150751268893E-5 0.0 2.4479046046736093 3.2729618589339896E-4 72-73 0.0013543290450761336 2.2572150751268893E-5 0.0 2.7392320683448608 3.7244048739593674E-4 74-75 0.0013769011958274025 2.2572150751268893E-5 0.0 3.052059505606697 4.062987135228401E-4 76-77 0.0013769011958274025 2.2572150751268893E-5 0.0 3.394445174277319 4.062987135228401E-4 78-79 0.0013994733465786714 2.2572150751268893E-5 0.0 3.791353873087631 4.2887086427410896E-4 80-81 0.001692911306345167 2.2572150751268893E-5 0.0 4.221172767693293 4.5144301502537785E-4 82-83 0.001692911306345167 2.2572150751268893E-5 0.0 4.660212385880849 4.8530124115228124E-4 84-85 0.0017267695324720703 2.2572150751268893E-5 0.0 5.111835978112237 4.965873165279157E-4 86-87 0.0018170581354771459 2.2572150751268893E-5 0.0 5.593536961219691 5.30445542654819E-4 88-89 0.0018509163616040492 2.2572150751268893E-5 0.0 6.119806655985525 5.643037687817224E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12020 0.0 21.759722 1 TACATGG 11445 0.0 21.563013 2 GTATCAA 22495 0.0 20.532084 1 ACATGGG 11540 0.0 20.318281 3 GAGTACT 9795 0.0 20.253881 12-13 GGTATCA 17975 0.0 18.538492 1 AGAGTAC 15495 0.0 18.36367 10-11 GTACTTT 10715 0.0 18.18417 14-15 TATCAAC 25205 0.0 18.11665 2 TCAACGC 25160 0.0 18.017265 4 ATCAACG 25195 0.0 17.973434 3 CAACGCA 25200 0.0 17.932074 5 AACGCAG 25590 0.0 17.687807 6 AGTACTT 10335 0.0 17.454731 12-13 GGACCGA 1270 0.0 17.149067 6 CATGGGG 8120 0.0 16.625565 4 GAGTACA 9740 0.0 16.375196 1 ACGCAGA 27700 0.0 16.032803 7 GTGTAGC 2695 0.0 16.02835 1 AGTACAT 9080 0.0 15.650311 2 >>END_MODULE