FastQCFastQC Report
Fri 27 May 2016
SRR939011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4430238
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103220.23298974005459752No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85930.1939624914056536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73340.16554415360980607No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45100.10180039988822272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG113000.021.5001181
TACATGG106900.021.1302052
GAGTACT103400.020.4007112-13
GTATCAA237000.020.1404171
ACATGGG110650.019.341923
AGAGTAC155750.019.33292610-11
GTACTTT111050.018.69620914-15
AGTACTT111750.018.2181312-13
TCAACGC260350.017.9536654
ATCAACG261500.017.8928573
CAACGCA262950.017.7761425
TATCAAC265850.017.7607332
AACGCAG267950.017.4444356
GGTATCA190350.017.2931561
GAGTACA92700.017.1638951
CATGGGG79650.017.0394154
AGTACAT86450.016.2480472
GTATAGG9150.016.1394861
GGACCGA12850.015.8796096
ACGCAGA289500.015.7524927
Produced by FastQC (version 0.11.5)