##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939011_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4430238 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.208673664936285 33.0 31.0 34.0 31.0 34.0 2 32.44535801462585 34.0 31.0 34.0 31.0 34.0 3 32.5448637296687 34.0 31.0 34.0 31.0 34.0 4 36.0824445548975 37.0 35.0 37.0 35.0 37.0 5 36.00468214123033 37.0 35.0 37.0 35.0 37.0 6 36.04041746741372 37.0 35.0 37.0 35.0 37.0 7 35.979047626786645 37.0 35.0 37.0 35.0 37.0 8 35.946433126166134 37.0 35.0 37.0 35.0 37.0 9 37.63692627800132 39.0 37.0 39.0 35.0 39.0 10-11 37.544435535968944 39.0 37.0 39.0 35.0 39.0 12-13 37.45760453501595 39.0 37.0 39.0 34.0 39.0 14-15 38.79970003868867 40.0 38.0 41.0 34.5 41.0 16-17 38.88310334568933 40.0 38.0 41.0 35.0 41.0 18-19 38.902475555489346 40.0 38.0 41.0 35.0 41.0 20-21 38.864230996167706 40.0 38.0 41.0 34.5 41.0 22-23 38.79143084412169 40.0 38.0 41.0 34.0 41.0 24-25 38.7208078888764 40.0 38.0 41.0 34.0 41.0 26-27 38.570659973572525 40.0 38.0 41.0 34.0 41.0 28-29 38.41551040824443 40.0 38.0 41.0 34.0 41.0 30-31 38.246043666277075 40.0 38.0 41.0 34.0 41.0 32-33 38.18159340423697 40.0 38.0 41.0 33.0 41.0 34-35 38.09777341533344 40.0 38.0 41.0 33.0 41.0 36-37 37.98732506018864 40.0 38.0 41.0 33.0 41.0 38-39 37.85237881125123 40.0 37.0 41.0 33.0 41.0 40-41 37.700410677710764 40.0 37.0 41.0 33.0 41.0 42-43 37.528577697180154 40.0 37.0 41.0 32.0 41.0 44-45 37.30626424584864 40.0 37.0 41.0 31.5 41.0 46-47 37.05435294898378 40.0 36.0 41.0 31.0 41.0 48-49 36.762752926592206 39.5 36.0 41.0 30.5 41.0 50-51 36.51814101183729 39.0 36.0 41.0 30.0 41.0 52-53 36.26179552881809 39.0 35.0 40.0 30.0 41.0 54-55 35.956468365807886 39.0 35.0 40.0 29.0 41.0 56-57 35.81023480002655 38.5 35.0 40.0 29.0 41.0 58-59 35.52660274685017 38.0 34.5 40.0 28.0 41.0 60-61 35.15589896073304 38.0 34.0 40.0 28.0 41.0 62-63 34.7297388989034 37.0 34.0 40.0 27.0 41.0 64-65 34.31913014605536 37.0 33.5 39.5 26.0 41.0 66-67 33.859124611363995 36.0 33.0 39.0 26.0 40.5 68-69 33.355950966968365 35.5 32.5 39.0 25.5 40.0 70-71 32.83206116691699 35.0 32.0 38.0 24.0 40.0 72-73 32.307620832108796 35.0 31.0 37.0 22.5 39.5 74-75 31.77267406401191 35.0 31.0 37.0 21.5 39.0 76-77 29.86850130850758 32.5 28.5 35.0 19.5 37.0 78-79 30.840366928368184 34.0 30.5 36.0 20.0 38.0 80-81 30.82151523236449 34.0 31.0 35.0 20.0 37.0 82-83 30.406770922916557 34.0 30.0 35.0 18.0 37.0 84-85 29.94496988649368 34.0 30.0 35.0 14.5 36.0 86-87 29.238434481398066 34.0 29.0 35.0 7.0 36.0 88-89 28.533437819819163 33.0 28.5 35.0 2.0 35.0 90-91 27.943286568351407 33.0 27.0 35.0 2.0 35.0 92-93 27.514262100591434 33.0 26.0 34.5 2.0 35.0 94-95 26.9404740783678 32.5 25.5 34.0 2.0 35.0 96-97 26.054763197823682 32.0 24.5 34.0 2.0 35.0 98-99 24.783495943107347 32.0 11.5 34.0 2.0 35.0 100-101 19.066719891798137 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17046729288995266 1101 2 0.19690500511758557 1101 3 0.24262662742285102 1101 4 0.10851449872161112 1101 5 0.12808745901387653 1101 6 0.17909214164120613 1101 7 0.20476374150918986 1101 8 0.13952247834297538 1101 9 0.27774438058129647 1101 10-11 0.25340510033177566 1101 12-13 0.2663902541710925 1101 14-15 0.3267867006432468 1101 16-17 0.30915285339344933 1101 18-19 0.3435283463433123 1101 20-21 0.31942334404536865 1101 22-23 0.29117562542863595 1101 24-25 0.23975279437175345 1101 26-27 0.23240810547680724 1101 28-29 0.2847570871754499 1101 30-31 0.19157313802939058 1101 32-33 0.30811813689306433 1101 34-35 0.3436177287565627 1101 36-37 0.41834352305093603 1101 38-39 0.3870357156144735 1101 40-41 0.4172560731535455 1101 42-43 0.3559658487505146 1101 44-45 0.39744219390567537 1101 46-47 0.4093959406739316 1101 48-49 0.3674315340917502 1101 50-51 0.28138912652407555 1101 52-53 0.24649729137829723 1101 54-55 0.25368181822053515 1101 56-57 0.20900893922125618 1101 58-59 0.27802939354916845 1101 60-61 0.31555633019692664 1101 62-63 0.33164531029377287 1101 64-65 0.32299908308566216 1101 66-67 0.20203359530543707 1101 68-69 0.24397971057864964 1101 70-71 0.3703950787270216 1101 72-73 0.36289703792041195 1101 74-75 0.42104732354600927 1101 76-77 0.009824510619779403 1101 78-79 0.38851075642413235 1101 80-81 0.3884975087874203 1101 82-83 0.29399376407995703 1101 84-85 0.39250996154859763 1101 86-87 0.5258529493950839 1101 88-89 0.5286762421594418 1101 90-91 0.6039515893426675 1101 92-93 0.651604009725073 1101 94-95 0.5480963511659738 1101 96-97 0.6406556119254567 1101 98-99 0.9362032118342398 1101 100-101 1.1765597517041044 1102 1 0.177127449098073 1102 2 0.1875588678295017 1102 3 0.16429766311704697 1102 4 0.06477403301337148 1102 5 0.11095765875185748 1102 6 0.1530873347976467 1102 7 0.15557656407886356 1102 8 0.14540446480633307 1102 9 0.1481790287365925 1102 10-11 0.24474963708581043 1102 12-13 0.20448716367277342 1102 14-15 0.2763981696919231 1102 16-17 0.293378879153984 1102 18-19 0.2520475688335111 1102 20-21 0.2815445245809869 1102 22-23 0.2991698975275341 1102 24-25 0.2982719334759878 1102 26-27 0.2647794010254927 1102 28-29 0.24552464546045627 1102 30-31 0.3392509937426027 1102 32-33 0.2766121792040579 1102 34-35 0.2555806129484566 1102 36-37 0.2804647393233992 1102 38-39 0.1670763436394509 1102 40-41 0.20180704806652727 1102 42-43 0.28698626782932024 1102 44-45 0.22464232069998502 1102 46-47 0.19512134735324338 1102 48-49 0.12308507278962821 1102 50-51 0.10356679747781072 1102 52-53 0.09712020836709456 1102 54-55 0.03475509208934824 1102 56-57 0.015528721112161747 1102 58-59 0.040639168195021114 1102 60-61 0.04198791236938604 1102 62-63 0.08641924617568009 1102 64-65 0.07757033150937787 1102 66-67 -0.10056826720349932 1102 68-69 -0.12569478515663945 1102 70-71 0.09528467570836341 1102 72-73 0.19106414411053407 1102 74-75 0.2653953274884948 1102 76-77 -0.05066075043427887 1102 78-79 0.19476423927723374 1102 80-81 0.1893640795681648 1102 82-83 0.21506465906629302 1102 84-85 0.3065601751477054 1102 86-87 0.4070445062563337 1102 88-89 0.26309810581671655 1102 90-91 0.3174638562520933 1102 92-93 0.4973356548658501 1102 94-95 0.6735321023984788 1102 96-97 0.6580199054354772 1102 98-99 0.8799546130162703 1102 100-101 0.8788883822277995 1103 1 0.09903593943328559 1103 2 0.14522662442654877 1103 3 0.12295237762202049 1103 4 0.07859292662282513 1103 5 0.09331875935910716 1103 6 0.07513587006467759 1103 7 0.13343878798170294 1103 8 0.08845552841526683 1103 9 0.058651406045285626 1103 10-11 0.1381655382061311 1103 12-13 0.1266207283382741 1103 14-15 0.1777009256587334 1103 16-17 0.142687744662517 1103 18-19 0.19357732689861962 1103 20-21 0.18890423867816963 1103 22-23 0.15511289491021785 1103 24-25 0.11012103372733861 1103 26-27 0.14877029602887148 1103 28-29 0.1181461875265768 1103 30-31 0.18266682071800489 1103 32-33 0.22516289803909473 1103 34-35 0.20523078236961112 1103 36-37 0.22385209464811595 1103 38-39 0.18716336012186474 1103 40-41 0.28261460491714274 1103 42-43 0.34119104127950806 1103 44-45 0.24220366450258268 1103 46-47 0.2755036978956156 1103 48-49 0.22956080144886215 1103 50-51 0.24309668900379222 1103 52-53 0.20833937334696628 1103 54-55 0.31335639259130943 1103 56-57 0.44789277268737493 1103 58-59 0.3893036846589055 1103 60-61 0.42175269501353796 1103 62-63 0.47529970231524743 1103 64-65 0.46123451432109874 1103 66-67 0.33150516592890256 1103 68-69 0.4067342766876436 1103 70-71 0.43033615452886664 1103 72-73 0.48447022844297294 1103 74-75 0.476678435050534 1103 76-77 0.22092356346566788 1103 78-79 0.520831844618634 1103 80-81 0.44867749757500164 1103 82-83 0.4700966822588235 1103 84-85 0.66915302312837 1103 86-87 0.8103861883080725 1103 88-89 0.6987899891719245 1103 90-91 0.8369430029923102 1103 92-93 0.7520301110499084 1103 94-95 0.7277021167084499 1103 96-97 0.5225666797605761 1103 98-99 0.8984727229138763 1103 100-101 1.183034892596968 1104 1 -0.011055013175621298 1104 2 0.024332532719625988 1104 3 0.014710939787470068 1104 4 0.05080529443691262 1104 5 0.018712553693063683 1104 6 0.033872455642814714 1104 7 0.05822472296581793 1104 8 0.11084872800329748 1104 9 0.11740737163705717 1104 10-11 0.093858968439541 1104 12-13 0.12022616518640916 1104 14-15 0.13561702296497202 1104 16-17 0.10639893023775215 1104 18-19 0.09155925242482255 1104 20-21 0.13980050628482132 1104 22-23 0.12931780664121106 1104 24-25 0.12429266869839495 1104 26-27 0.027484310273763413 1104 28-29 0.15179314054164905 1104 30-31 0.09466919970171972 1104 32-33 0.1278204259268776 1104 34-35 0.17193087085620107 1104 36-37 0.1889359828358863 1104 38-39 0.0743453958936584 1104 40-41 0.16309951142598322 1104 42-43 0.01690373547855728 1104 44-45 0.17319860190920622 1104 46-47 0.22564794554201484 1104 48-49 0.14144141708343483 1104 50-51 0.18757219562577632 1104 52-53 0.1290600313390442 1104 54-55 0.3280423812476201 1104 56-57 0.19895919565326636 1104 58-59 0.21098284099004871 1104 60-61 0.1587222179112615 1104 62-63 0.09496023294466482 1104 64-65 0.2016629634848215 1104 66-67 0.1678336687648283 1104 68-69 0.19196891120901682 1104 70-71 0.1978453875967361 1104 72-73 0.20291701318846123 1104 74-75 0.2634357932181395 1104 76-77 0.22352585251218926 1104 78-79 0.2758508039583205 1104 80-81 0.37833181963717166 1104 82-83 0.3736437179580854 1104 84-85 0.4972066082858291 1104 86-87 0.4293315732247329 1104 88-89 0.44687302150579455 1104 90-91 0.600062459887738 1104 92-93 0.5718168438639282 1104 94-95 0.8719307892645496 1104 96-97 0.8294079116099446 1104 98-99 0.8095780509670263 1104 100-101 1.1798570925766576 1105 1 0.04345788433556663 1105 2 0.0783840903026416 1105 3 0.09742762217967282 1105 4 0.06000577419437292 1105 5 -0.00301550868037026 1105 6 0.04435444146267287 1105 7 0.04010847995678546 1105 8 0.052990636976744554 1105 9 0.07952232384108981 1105 10-11 0.07884611843746114 1105 12-13 0.09235942445888412 1105 14-15 0.07795976077229483 1105 16-17 0.062980029412536 1105 18-19 0.060578918039603025 1105 20-21 0.03045961823307408 1105 22-23 0.007003156128476462 1105 24-25 -0.05582070054498445 1105 26-27 -0.10783662079655443 1105 28-29 0.11173495963665658 1105 30-31 0.11251165949903452 1105 32-33 0.05757505349998837 1105 34-35 0.0870474800742258 1105 36-37 0.10518097564076356 1105 38-39 0.1364877602331731 1105 40-41 0.17894873025338853 1105 42-43 0.24840888489104174 1105 44-45 0.24226148785025714 1105 46-47 0.3105238689967891 1105 48-49 0.18537040680309502 1105 50-51 0.16351455259377445 1105 52-53 0.21193592278293494 1105 54-55 0.2984387965698758 1105 56-57 0.20148830893042913 1105 58-59 0.24614386417956524 1105 60-61 0.2835315192816523 1105 62-63 0.2357760742284043 1105 64-65 0.12304119425916582 1105 66-67 0.20528518890412784 1105 68-69 0.26505894079992487 1105 70-71 0.37366679077120324 1105 72-73 0.34685842403201406 1105 74-75 0.29041516186661553 1105 76-77 0.17466639797986971 1105 78-79 0.284168645640392 1105 80-81 0.2731870622588062 1105 82-83 0.3307880508541281 1105 84-85 0.3481702260024093 1105 86-87 0.38051500651129544 1105 88-89 0.5529351611649886 1105 90-91 0.4802303716161518 1105 92-93 0.6669063802167372 1105 94-95 0.5675142907207622 1105 96-97 0.7226963399128401 1105 98-99 0.8780830289409494 1105 100-101 1.029711234542436 1106 1 -0.008954822691428888 1106 2 0.024639319506682966 1106 3 0.0021335659487036196 1106 4 -0.017754753410770263 1106 5 0.0016177305957185695 1106 6 0.011221530362810483 1106 7 0.08490467082864939 1106 8 0.04170208170801004 1106 9 0.04638462740437177 1106 10-11 0.09034805622699338 1106 12-13 0.0582195806022483 1106 14-15 -0.01602403759971338 1106 16-17 -0.16611441084172185 1106 18-19 -0.07838642143926222 1106 20-21 -0.010319499861971337 1106 22-23 0.0422224197195078 1106 24-25 0.0479543320687128 1106 26-27 -0.07149522290177401 1106 28-29 -0.047051517001385434 1106 30-31 -0.016523045612736098 1106 32-33 0.003156039616534656 1106 34-35 -0.08847500529922314 1106 36-37 -0.0773910994172482 1106 38-39 -0.04677911310929517 1106 40-41 0.144325431427049 1106 42-43 0.11065230518880753 1106 44-45 0.14804424116995563 1106 46-47 0.07949753931209358 1106 48-49 0.11097013730161365 1106 50-51 0.06181570363042965 1106 52-53 0.0678039964836401 1106 54-55 0.08966866076962532 1106 56-57 0.13998123234164694 1106 58-59 0.07520276786976154 1106 60-61 0.14529849947851403 1106 62-63 0.18739194110032997 1106 64-65 0.15249366471650916 1106 66-67 0.15568922359564397 1106 68-69 0.15889607947300277 1106 70-71 0.25506482693420907 1106 72-73 0.21363266108345869 1106 74-75 0.14783974794857002 1106 76-77 -0.0453044440925936 1106 78-79 0.23019037215730265 1106 80-81 0.20418593063539703 1106 82-83 0.18274865230571535 1106 84-85 0.33388183550152206 1106 86-87 0.502022132041013 1106 88-89 0.6219266677358561 1106 90-91 0.751898703181265 1106 92-93 0.9470217829653684 1106 94-95 1.0850136150638257 1106 96-97 0.9499554403456365 1106 98-99 1.0216441134940446 1106 100-101 1.3528756092321395 1107 1 0.005804316687097355 1107 2 0.019771070271964675 1107 3 0.0014981684008148477 1107 4 0.009591791835276808 1107 5 -0.010619037471897741 1107 6 -0.018314472473043963 1107 7 -0.008651238496668157 1107 8 -0.006148766965729635 1107 9 -0.06277338275814515 1107 10-11 0.031196345586458563 1107 12-13 -0.004165973632893838 1107 14-15 0.05071475325508601 1107 16-17 0.02204142011327548 1107 18-19 -0.01969884676589828 1107 20-21 0.03417402390910951 1107 22-23 0.018856264808917444 1107 24-25 -0.05961166076264135 1107 26-27 -0.04304401910583522 1107 28-29 -0.01502197117556392 1107 30-31 0.005781266143131347 1107 32-33 0.004155795109660687 1107 34-35 0.06793224918758511 1107 36-37 0.004955024081141346 1107 38-39 -0.10160636206532558 1107 40-41 -0.10880169399430173 1107 42-43 -0.00870665972499296 1107 44-45 -0.024653310237880532 1107 46-47 -0.15382778615431647 1107 48-49 -0.14015945240731043 1107 50-51 -0.19745254816680813 1107 52-53 -0.18938979069287853 1107 54-55 -0.25782370239461017 1107 56-57 -0.07780285207621063 1107 58-59 -0.044080335851326424 1107 60-61 0.03637682816194854 1107 62-63 0.20224594700906806 1107 64-65 0.11769750763846076 1107 66-67 0.10845183091440447 1107 68-69 0.07224850356109158 1107 70-71 0.1481344985796298 1107 72-73 0.22637221642262517 1107 74-75 0.24128306666488086 1107 76-77 0.17807970182569122 1107 78-79 0.33614256625945416 1107 80-81 0.2594672256462829 1107 82-83 0.1444676316977329 1107 84-85 0.34755297269336793 1107 86-87 0.29410123054738335 1107 88-89 0.4997986892097508 1107 90-91 0.6299167347621584 1107 92-93 0.8261120679279799 1107 94-95 0.8194512866323755 1107 96-97 0.9080202165150801 1107 98-99 0.8829052030550955 1107 100-101 0.8513343083915252 1108 1 0.09779644868142157 1108 2 0.1416206002161502 1108 3 0.14265495225132696 1108 4 0.07318284211208947 1108 5 0.09161561695742648 1108 6 0.1537840477337511 1108 7 0.1458151773861971 1108 8 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5 75.0 6 172.0 7 422.0 8 1103.0 9 2154.0 10 3504.0 11 5373.0 12 7940.0 13 10637.0 14 13150.0 15 17473.0 16 22169.0 17 29888.0 18 30023.0 19 23932.0 20 24221.0 21 26634.0 22 29230.0 23 32447.0 24 38257.0 25 45766.0 26 54542.0 27 65891.0 28 79983.0 29 97624.0 30 118135.0 31 144176.0 32 178518.0 33 227531.0 34 306600.0 35 438975.0 36 690583.0 37 1008044.0 38 625630.0 39 29390.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.10038023468541 19.609925759172008 14.452362569714792 23.837331436427792 2 15.742788536417232 20.819242668226853 39.321070335273184 24.11689846008273 3 19.554096190768984 23.91921156380312 29.85886988464277 26.66782236078513 4 13.303506493330609 16.905502593765842 36.84804292681341 32.94294798609014 5 13.871263801177273 37.15297011131231 34.627123870094564 14.348642217415858 6 32.33948153575496 36.26466569064687 17.150500718020115 14.245352055578053 7 28.50196761438099 32.255625995714 20.98923353553466 18.25317285437035 8 25.64634225068721 35.70717871139203 19.702237216149562 18.9442418217712 9 25.58336595009117 17.13384247076568 20.64541453529133 36.63737704385182 10-11 24.917002201687584 26.411278581421588 27.522731284414064 21.148987932476764 12-13 25.772712436668186 23.636822671829368 27.795052997152748 22.795411894349694 14-15 22.587511518942982 25.24526813390584 26.0440916639815 26.12312868316967 16-17 22.368368036443204 28.28887617543201 26.6888541444584 22.653901643666384 18-19 22.274999887138787 27.534321094663472 28.246949361431263 21.943729656766482 20-21 23.02969953307249 26.519861912610565 28.211813902548805 22.238624651768145 22-23 22.683442740548024 26.44812761752303 28.116559426378444 22.751870215550497 24-25 22.74948783766824 26.48656866135348 28.213101965674063 22.550841535304215 26-27 22.536553357428364 26.58830139071598 28.412154566935033 22.462990684920623 28-29 22.361970169548453 27.095022885903646 28.17548402591463 22.367522918633263 30-31 22.628626272448567 26.66427853311718 28.064225894861632 22.642869299572617 32-33 22.530708282489563 26.69934436930928 28.061584953223733 22.708362394977424 34-35 22.915475832500316 26.68543105262065 28.05881840354627 22.340274711332768 36-37 22.824696238086755 26.456936215924788 28.224204366964845 22.494163179023612 38-39 22.540555891290502 26.879479230322428 28.074851159805597 22.505113718581473 40-41 22.843213097302222 26.772157713543244 27.939769944837234 22.4448592443173 42-43 22.56290803300257 26.766964968656453 27.789319615708152 22.880807382632824 44-45 22.79485806033511 26.644021871008185 27.80071902643631 22.760401042220398 46-47 22.96387597620143 26.942177544850303 27.435690000997702 22.658256477950566 48-49 22.784708977098546 26.8274489714034 27.58716274242315 22.800679309074905 50-51 22.564130982037987 27.11844053459762 27.619511533368446 22.697916949995943 52-53 22.91885898680838 27.17042515548826 27.15644170809785 22.754274149605507 54-55 22.826832328195458 26.948823065487677 27.26086047747322 22.963484128843643 56-57 22.765921379393163 27.041594605075392 27.366159560727887 22.826324454803558 58-59 22.97974482650507 26.882738855106254 27.297437825344968 22.840078493043713 60-61 22.807434144048987 27.0088318071916 27.54002912522413 22.64370492353528 62-63 22.624821338765177 26.95211382312019 27.596671190193067 22.82639364792157 64-65 22.45949254012655 27.187933282959413 27.43301237358789 22.919561803326147 66-67 22.60231463177703 26.97981308187597 27.17419826973143 23.24367401661557 68-69 22.530249717615128 26.909088774941743 27.468689363999744 23.09197214344339 70-71 22.793356832457683 26.694593696588626 27.294482731845438 23.217566739108257 72-73 22.73779647955708 26.61616599379085 27.334919704088133 23.311117822563933 74-75 22.33325839379284 27.30007958940355 27.086366466090535 23.280295550713078 76-77 22.493443485192035 26.82352220855591 27.21539935998294 23.46763494626911 78-79 22.610118928035966 26.86549931220111 27.249127215482176 23.27525454428075 80-81 22.710112727807072 26.788998943395477 27.136599485037905 23.364288843759542 82-83 22.568223197038172 26.71420813057899 27.403900196784008 23.313668475598828 84-85 22.529145226837084 26.80383492678029 27.19057574463436 23.47644410174826 86-87 22.352257135427223 26.763107815221414 27.312765427228687 23.571869622122676 88-89 22.48357334172482 26.749890836574984 27.02483983696448 23.74169598473571 90-91 22.442344814724212 26.55882663861554 27.16774670230616 23.831081844354085 92-93 22.465373192140014 26.625002990809975 27.164127525428654 23.745496291621354 94-95 22.400466436082073 26.54566204737541 27.16429253921221 23.889578977330313 96-97 22.136680031262433 26.484415338906775 27.37252799652389 24.006376633306907 98-99 22.20218191438022 26.575885990775213 27.001337174210505 24.22059492063406 100-101 22.426707928725886 25.722763203633225 27.77382387330652 24.076704994334367 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 416.0 1 297.0 2 221.5 3 280.5 4 261.0 5 281.5 6 508.5 7 916.0 8 1500.5 9 1982.0 10 1979.0 11 1931.0 12 2166.0 13 2430.5 14 2762.0 15 3049.0 16 3365.5 17 3785.0 18 4216.5 19 4707.0 20 5411.5 21 6549.5 22 8133.0 23 10491.5 24 13211.0 25 16667.5 26 22306.5 27 29414.5 28 35242.5 29 40863.5 30 47217.0 31 54723.5 32 62862.5 33 70596.5 34 79428.0 35 88723.5 36 98890.0 37 108194.0 38 115739.5 39 120731.5 40 125686.0 41 132374.0 42 137903.0 43 145611.0 44 154473.0 45 159496.0 46 162990.0 47 173952.0 48 188593.0 49 186866.5 50 174649.0 51 167612.0 52 163473.0 53 160162.0 54 155576.5 55 145733.0 56 133331.0 57 121325.0 58 111526.5 59 101504.0 60 84754.5 61 67704.0 62 53963.0 63 41109.5 64 31010.5 65 23034.5 66 16372.0 67 11865.0 68 8465.5 69 5903.0 70 4056.5 71 2608.5 72 1615.0 73 1018.0 74 690.0 75 444.5 76 267.0 77 129.0 78 54.0 79 22.0 80 16.5 81 10.0 82 10.5 83 11.5 84 6.5 85 3.5 86 2.5 87 3.5 88 3.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3935905926498757 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.013113516700457E-4 16-17 0.0025167948087664815 18-19 4.0629871352284013E-4 20-21 0.0 22-23 0.0 24-25 4.514430150253779E-5 26-27 4.514430150253779E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01286612592822327 36-37 0.02322674312305569 38-39 0.01099263741586795 40-41 0.005801042743076105 42-43 0.004954587089903522 44-45 0.0018283442108527803 46-47 0.001264040442071058 48-49 0.003351964386563431 50-51 6.207341456598946E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.238835024213146E-4 60-61 0.0012978986681979614 62-63 0.001489761949583747 64-65 8.013113516700457E-4 66-67 0.002629655562522826 68-69 0.002573225185644654 70-71 4.514430150253779E-5 72-73 0.0 74-75 0.0 76-77 0.09268125098471008 78-79 0.003701832723208099 80-81 2.0314935676142006E-4 82-83 0.0 84-85 2.2572150751268893E-4 86-87 0.09297468894447658 88-89 0.43835116758964193 90-91 0.39148009655463206 92-93 0.0 94-95 0.0011060353868121758 96-97 1.1286075375634447E-5 98-99 0.0 100-101 4.0629871352284013E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4430238.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.57774095695223 #Duplication Level Percentage of deduplicated Percentage of total 1 67.86892666827285 31.611812853812005 2 15.813555547020561 14.731193877549975 3 6.28208514932336 8.778160042641002 4 3.151445937831195 5.871489301285633 5 1.913322003515478 4.45591083234904 6 1.1538597936298947 3.2246509541001362 7 0.7768237711748539 2.532788746808956 8 0.5602696888131701 2.0876877145257664 9 0.41616335639015034 1.7445554108744594 >10 1.9132945035620172 15.256742491074101 >50 0.09817886038605325 3.1412010976622042 >100 0.04843351424679682 4.101295179577383 >500 0.0023789213139159063 0.7679675501507346 >1k 0.001116636305867458 1.1009696431121678 >5k 9.709880920585068E-5 0.3601606898319085 >10k+ 4.854940460292534E-5 0.2334136146446261 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10322 0.23298974005459752 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8593 0.1939624914056536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7334 0.16554415360980607 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4510 0.10180039988822272 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.1286075375634446E-4 0.0 0.0 0.0 0.0 6 1.5800505525888226E-4 0.0 0.0 0.0 0.0 7 2.0314935676142004E-4 0.0 0.0 0.0 0.0 8 2.2572150751268893E-4 0.0 0.0 0.0 0.0 9 2.2572150751268893E-4 0.0 0.0 0.0 0.0 10-11 2.4829365826395784E-4 0.0 0.0 4.514430150253779E-5 0.0 12-13 2.7086580901522673E-4 0.0 0.0 6.771645225380668E-5 1.1286075375634446E-4 14-15 3.611544120203023E-4 0.0 0.0 9.028860300507557E-5 1.1286075375634446E-4 16-17 3.837265627715712E-4 0.0 0.0 1.5800505525888226E-4 1.3543290450761337E-4 18-19 3.9501263814720563E-4 0.0 0.0 1.8057720601015115E-4 1.5800505525888226E-4 20-21 4.062987135228401E-4 0.0 0.0 2.4829365826395784E-4 1.692911306345167E-4 22-23 4.5144301502537785E-4 0.0 0.0 4.401569396497434E-4 2.0314935676142004E-4 24-25 4.965873165279157E-4 0.0 0.0 6.997366732893357E-4 2.1443543213705448E-4 26-27 4.965873165279157E-4 0.0 0.0 0.0011173214621878103 2.2572150751268893E-4 28-29 5.530176934060879E-4 0.0 0.0 0.0035776858940761193 2.2572150751268893E-4 30-31 5.868759195329912E-4 0.0 0.0 0.009548019767786742 2.2572150751268893E-4 32-33 6.094480702842601E-4 0.0 0.0 0.020529371108279057 2.4829365826395784E-4 34-35 6.771645225380668E-4 0.0 0.0 0.03971569924685762 2.7086580901522673E-4 36-37 7.448809747918735E-4 0.0 0.0 0.07035739389170514 2.7086580901522673E-4 38-39 7.787392009187768E-4 0.0 0.0 0.11651744217805002 3.0472403514213007E-4 40-41 7.900252762944113E-4 0.0 0.0 0.1686703964888568 3.160101105177645E-4 42-43 8.915999546751213E-4 0.0 0.0 0.22572150751268893 3.160101105177645E-4 44-45 0.0010157467838071003 0.0 0.0 0.2992051442834448 3.160101105177645E-4 46-47 0.0011173214621878103 0.0 0.0 0.38575805633918536 3.2729618589339896E-4 48-49 0.0011511796883147136 0.0 0.0 0.4789810389419259 3.385822612690334E-4 50-51 0.0011511796883147136 0.0 0.0 0.5806572920010167 3.611544120203023E-4 52-53 0.0011511796883147136 0.0 0.0 0.6878298637680413 4.062987135228401E-4 54-55 0.0011963239898172514 0.0 0.0 0.8112543840759796 4.062987135228401E-4 56-57 0.001264040442071058 0.0 0.0 0.9595082702103137 4.175847888984745E-4 58-59 0.001264040442071058 0.0 0.0 1.120323558237729 4.2887086427410896E-4 60-61 0.0012753265174466925 0.0 0.0 1.2962847594192457 4.2887086427410896E-4 62-63 0.0013091847435735958 0.0 0.0 1.4793223298612852 4.5144301502537785E-4 64-65 0.0013317568943248647 0.0 0.0 1.6817493777986647 4.5144301502537785E-4 66-67 0.0014220454973299403 0.0 0.0 1.918012079712196 4.5144301502537785E-4 68-69 0.001467189798832478 0.0 0.0 2.1868915394613113 4.5144301502537785E-4 70-71 0.001489761949583747 0.0 0.0 2.4735691400778017 4.5144301502537785E-4 72-73 0.0015010480249593814 0.0 0.0 2.768372714964749 4.740151657766468E-4 74-75 0.0015123341003350158 0.0 0.0 3.0837620913368538 5.191594672791846E-4 76-77 0.0015123341003350158 0.0 0.0 3.4284162611579783 5.417316180304535E-4 78-79 0.0015236201757106503 0.0 0.0 3.826803435842499 5.643037687817224E-4 80-81 0.0018057720601015114 0.0 0.0 4.25874411261878 5.755898441573568E-4 82-83 0.001896060663106587 0.0 0.0 4.699521786414184 6.771645225380668E-4 84-85 0.0019299188892334903 0.0 0.0 5.153097869685556 6.771645225380668E-4 86-87 0.001963777115360394 0.0 0.0 5.638353966536335 6.771645225380668E-4 88-89 0.001963777115360394 0.0 0.0 6.1671404561109355 6.771645225380668E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11300 0.0 21.500118 1 TACATGG 10690 0.0 21.130205 2 GAGTACT 10340 0.0 20.40071 12-13 GTATCAA 23700 0.0 20.140417 1 ACATGGG 11065 0.0 19.34192 3 AGAGTAC 15575 0.0 19.332926 10-11 GTACTTT 11105 0.0 18.696209 14-15 AGTACTT 11175 0.0 18.21813 12-13 TCAACGC 26035 0.0 17.953665 4 ATCAACG 26150 0.0 17.892857 3 CAACGCA 26295 0.0 17.776142 5 TATCAAC 26585 0.0 17.760733 2 AACGCAG 26795 0.0 17.444435 6 GGTATCA 19035 0.0 17.293156 1 GAGTACA 9270 0.0 17.163895 1 CATGGGG 7965 0.0 17.039415 4 AGTACAT 8645 0.0 16.248047 2 GTATAGG 915 0.0 16.139486 1 GGACCGA 1285 0.0 15.879609 6 ACGCAGA 28950 0.0 15.752492 7 >>END_MODULE