FastQCFastQC Report
Fri 27 May 2016
SRR939010_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939010_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4441721
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT93880.2113595158273111No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86750.19530717935683037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67950.1529812430812291No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50500.11369466925095024No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118650.023.136351
TACATGG111500.023.0915832
ACATGGG115650.021.0761493
GTATCAA231750.020.8467561
GAGTACT104700.019.97898712-13
AGAGTAC160050.019.13106510-11
GTACTTT110750.018.71718414-15
CATGGGG80800.018.5099564
ATCAACG260150.018.411383
CAACGCA260100.018.3965175
TCAACGC261500.018.3163324
TATCAAC263200.018.2699572
AACGCAG264350.018.1341086
GGTATCA182000.017.8965531
AGTACTT111950.017.56486512-13
GTATAGG9100.017.1931951
GGACCGA13500.016.8257166
ACGCAGA286250.016.4326277
CGCAGAG287850.016.0459088
GAGTACA96750.015.3383071
Produced by FastQC (version 0.11.5)