FastQCFastQC Report
Fri 27 May 2016
SRR939010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4441721
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103320.23261253914867686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84070.18927348205796807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74330.16734504485986398No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46610.10493680264924339No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG3850.027.1150155
TACATGG108300.022.389292
GTACATG113500.022.0353951
ACATGGG112350.020.2306733
GTATCAA226100.019.868661
GAGTACT105400.019.2478612-13
AGAGTAC155350.018.75616810-11
GGTATCA180250.018.0246371
GTACTTT113200.017.69102514-15
CAACGCA251650.017.4795675
TCAACGC252500.017.4019344
TATCAAC254400.017.3838792
ATCAACG252700.017.369383
CATGGGG75800.017.027374
AACGCAG258750.016.9999356
AGTACTT112750.016.85671612-13
GAGTACA93250.016.1434251
AGTACAT86150.015.6977522
ATACCGA3654.2537067E-815.6004196
ACGCAGA280600.015.422517
Produced by FastQC (version 0.11.5)