FastQCFastQC Report
Fri 27 May 2016
SRR939009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980633
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137820.4623850034539643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104540.35073086824174593No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT102300.3432156860640005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67830.22756911031985488No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55270.18543041025178209No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA48250.16187836610545478No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44200.1482906483287275No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA38150.12799294646472748No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37590.12611415092029107No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30200.10132075971781833No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT300.003925435231.71076468-69
GTACATG120400.024.4011941
TACATGG121500.023.2758452
GAGTACT106750.022.51016212-13
ACATGGG119950.021.9574493
CATGGGG51150.020.5596034
GTACTTT118200.020.20994414-15
AGTACTT112150.020.18083612-13
ATGGGGA30600.018.8859355
GTATCAA316550.017.4075181
GTATAGG11250.017.29731
AGTACAT92550.017.0953272
CATGGGA70400.017.0910384
GTGTAGC15800.016.822011
ACTTTTT145450.016.60406116-17
TCAACGC347950.015.7243434
CAACGCA347300.015.712595
ATCAACG349250.015.6658143
AGAGTAC228950.015.6643510-11
TATCAAC351300.015.6283332
Produced by FastQC (version 0.11.5)