##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939009_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2980633 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81194195997964 33.0 31.0 34.0 30.0 34.0 2 31.891052672368588 33.0 31.0 34.0 30.0 34.0 3 31.93314272505203 34.0 31.0 34.0 30.0 34.0 4 35.47229330145644 37.0 35.0 37.0 33.0 37.0 5 35.518000706561324 37.0 35.0 37.0 33.0 37.0 6 35.60867070853742 37.0 35.0 37.0 33.0 37.0 7 35.53483203064584 37.0 35.0 37.0 33.0 37.0 8 35.528812839420354 37.0 35.0 37.0 33.0 37.0 9 37.129623808097136 39.0 37.0 39.0 33.0 39.0 10-11 37.02029065638071 39.0 37.0 39.0 33.0 39.0 12-13 36.91622350017597 39.0 37.0 39.0 33.0 39.0 14-15 38.11290420524767 40.0 38.0 41.0 33.0 41.0 16-17 38.03806976571755 40.0 38.0 41.0 33.0 41.0 18-19 37.978268374536555 40.0 38.0 41.0 33.0 41.0 20-21 38.09028954587834 40.0 38.0 41.0 33.0 41.0 22-23 38.02441209635671 40.0 38.0 41.0 33.0 41.0 24-25 37.94510662667963 40.0 38.0 41.0 33.0 41.0 26-27 37.82698205381206 40.0 38.0 41.0 32.5 41.0 28-29 37.69883830045497 40.0 37.5 41.0 32.0 41.0 30-31 37.50499289915934 40.0 37.0 41.0 32.0 41.0 32-33 37.30140107822734 40.0 37.0 41.0 31.5 41.0 34-35 36.974604052226496 40.0 36.0 41.0 30.5 41.0 36-37 36.7070595407083 39.5 36.0 41.0 30.0 41.0 38-39 36.465125025456004 39.0 35.5 41.0 30.0 41.0 40-41 36.32485633085321 39.0 35.0 41.0 30.0 41.0 42-43 36.1140355085648 39.0 35.0 41.0 29.5 41.0 44-45 36.02420509334762 39.0 35.0 41.0 29.0 41.0 46-47 35.791919031964014 39.0 35.0 41.0 27.5 41.0 48-49 35.53865621832678 39.0 35.0 41.0 26.5 41.0 50-51 34.429953972864155 38.0 34.0 40.0 24.5 40.5 52-53 34.53659088522472 38.0 34.5 39.5 24.5 40.5 54-55 35.04659664574606 39.0 35.0 40.0 26.0 41.0 56-57 34.964985122287786 39.0 35.0 41.0 25.0 41.0 58-59 34.76842486143045 38.5 34.5 40.0 25.0 41.0 60-61 34.460603167179585 38.0 34.0 40.0 23.5 41.0 62-63 34.094997270713975 38.0 34.0 40.0 22.5 41.0 64-65 33.71309064215554 37.0 33.5 40.0 22.0 41.0 66-67 33.3011900492278 36.5 33.0 39.5 20.5 41.0 68-69 32.8619142309704 36.0 32.5 39.0 19.5 41.0 70-71 32.410615127726224 35.5 32.0 39.0 18.0 40.0 72-73 31.9664703772655 35.0 31.5 38.0 15.5 40.0 74-75 31.50017076909502 35.0 31.0 37.0 12.0 39.5 76-77 31.065945388110514 35.0 31.0 37.0 7.5 39.0 78-79 30.663865527892902 35.0 31.0 36.0 6.5 39.0 80-81 30.20076037539677 34.5 30.0 36.0 2.0 37.5 82-83 29.72269883611971 34.0 30.0 35.0 2.0 37.0 84-85 29.303304532963303 34.0 29.0 35.0 2.0 37.0 86-87 28.85882646404304 34.0 29.0 35.0 2.0 36.0 88-89 28.371179041498905 34.0 29.0 35.0 2.0 36.0 90-91 27.905296458839445 34.0 27.5 35.0 2.0 35.5 92-93 27.406340364613825 34.0 26.5 35.0 2.0 35.0 94-95 26.8671731809988 33.0 25.5 35.0 2.0 35.0 96-97 26.226262508668462 33.0 24.5 35.0 2.0 35.0 98-99 25.375715493990704 33.0 20.5 35.0 2.0 35.0 100-101 22.1858086856047 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17174249975307987 1101 2 0.3657158500300888 1101 3 0.3484379772871833 1101 4 0.32616958402098817 1101 5 0.14793360377445453 1101 6 0.30535476647497717 1101 7 0.3199366859598598 1101 8 0.33567514394696474 1101 9 0.44698532396790824 1101 10-11 0.33271345081299586 1101 12-13 0.3012183714395533 1101 14-15 0.464687520619421 1101 16-17 0.48129840869409435 1101 18-19 0.6296277099005181 1101 20-21 0.5875772581652825 1101 22-23 0.6606244902375025 1101 24-25 0.5522953516103115 1101 26-27 0.5751827882358072 1101 28-29 0.564180248673253 1101 30-31 0.5428864237460473 1101 32-33 0.5230030550720244 1101 34-35 0.4574289377753402 1101 36-37 0.5386762729151826 1101 38-39 0.7241832299004898 1101 40-41 0.4580558463077651 1101 42-43 0.3677860857784836 1101 44-45 0.5950755189497343 1101 46-47 0.557246511524049 1101 48-49 0.6582221904222578 1101 50-51 0.47089616201264306 1101 52-53 0.4192185625407845 1101 54-55 0.5672172983133024 1101 56-57 0.45846120932183965 1101 58-59 0.4515830801838163 1101 60-61 0.5688428260670833 1101 62-63 0.48199935612630895 1101 64-65 0.34737700498114066 1101 66-67 0.3270922664855149 1101 68-69 0.40690784616340636 1101 70-71 0.22609711221758033 1101 72-73 0.1808384166610182 1101 74-75 0.0038096711484172374 1101 76-77 -0.1580220831406507 1101 78-79 -0.05513131317565012 1101 80-81 0.03390053327700571 1101 82-83 -0.031167376084997755 1101 84-85 -0.04638194125431383 1101 86-87 -0.1168648820899385 1101 88-89 -0.2288938614135283 1101 90-91 -0.1306249023288082 1101 92-93 -0.1539903572713115 1101 94-95 -0.4952237529891512 1101 96-97 -0.4268345701032281 1101 98-99 -0.592062721261513 1101 100-101 -0.3052149030090483 1102 1 0.21663832859084664 1102 2 0.29503320793436316 1102 3 0.4167785215019322 1102 4 0.316957638054042 1102 5 0.32518938686538945 1102 6 0.30745059747061987 1102 7 0.395045388648569 1102 8 0.3295654054807784 1102 9 0.4777468362030248 1102 10-11 0.40562355395917393 1102 12-13 0.45381415388312973 1102 14-15 0.5881349333335848 1102 16-17 0.5886046859372058 1102 18-19 0.5873318443789586 1102 20-21 0.5465477924538291 1102 22-23 0.5389163205426328 1102 24-25 0.6316499327678002 1102 26-27 0.6846635238536081 1102 28-29 0.6421034878515428 1102 30-31 0.5850320475861963 1102 32-33 0.6442203093985555 1102 34-35 0.6728527164210547 1102 36-37 0.5987354524931021 1102 38-39 0.7021260247733849 1102 40-41 0.6014586990115731 1102 42-43 0.6724743275462046 1102 44-45 0.8019236534713272 1102 46-47 0.7111089635114496 1102 48-49 0.6305214815620488 1102 50-51 0.4627686804624247 1102 52-53 0.5669057307683119 1102 54-55 0.6292197744688437 1102 56-57 0.5921037474082382 1102 58-59 0.5485588257278167 1102 60-61 0.5960894382115711 1102 62-63 0.5473876647879976 1102 64-65 0.41015415637728125 1102 66-67 0.4591185601284309 1102 68-69 0.6210657644664579 1102 70-71 0.6274738331187066 1102 72-73 0.5303999645009867 1102 74-75 0.48851293708428045 1102 76-77 0.4354983734645863 1102 78-79 0.4465333484703713 1102 80-81 0.4286691826663258 1102 82-83 0.41497071139503916 1102 84-85 0.4383310350954339 1102 86-87 0.36928671904941623 1102 88-89 0.1755657810274549 1102 90-91 0.0805651358654842 1102 92-93 0.14227679779320823 1102 94-95 0.02227603122387478 1102 96-97 -0.19132399114677057 1102 98-99 -0.3073104986556672 1102 100-101 -0.07704485747799694 1103 1 0.2658214237377976 1103 2 0.2939076709516719 1103 3 0.356183746715665 1103 4 0.30738552287391485 1103 5 0.17050354832172587 1103 6 0.3281297476556375 1103 7 0.2804195220329362 1103 8 0.248228743918105 1103 9 0.38499131750965176 1103 10-11 0.42546958347727326 1103 12-13 0.43700885529948863 1103 14-15 0.41653865924912026 1103 16-17 0.4747954583416103 1103 18-19 0.5759876863252842 1103 20-21 0.5690189229884766 1103 22-23 0.49443076059196756 1103 24-25 0.5838223201609338 1103 26-27 0.5612334007197504 1103 28-29 0.508862797323701 1103 30-31 0.49091945135464954 1103 32-33 0.5127935304440214 1103 34-35 0.41361674708755203 1103 36-37 0.4315155873236094 1103 38-39 0.6489737768894202 1103 40-41 0.4543663805223588 1103 42-43 0.41761250754705515 1103 44-45 0.5795249624330907 1103 46-47 0.4248510682709252 1103 48-49 0.5229990718389388 1103 50-51 0.38438555660551543 1103 52-53 0.44637185832561954 1103 54-55 0.5481844346279203 1103 56-57 0.42228935201885065 1103 58-59 0.5704092361739939 1103 60-61 0.6546808954018317 1103 62-63 0.3940716637671926 1103 64-65 0.3856902086506864 1103 66-67 0.5290408837216418 1103 68-69 0.48973614747866634 1103 70-71 0.5963798770981228 1103 72-73 0.4419678210362292 1103 74-75 0.21616714681589855 1103 76-77 0.2875054127228083 1103 78-79 0.2708439105477005 1103 80-81 0.36242372841087445 1103 82-83 0.3515258079455883 1103 84-85 0.40850045047546857 1103 86-87 0.5668966144175442 1103 88-89 0.34755774755376123 1103 90-91 0.45924758769654517 1103 92-93 0.32944743068845384 1103 94-95 0.29153350644823917 1103 96-97 0.18906377947123687 1103 98-99 0.31556310306267576 1103 100-101 0.5505394381708477 1104 1 0.162631407867444 1104 2 0.19594977156729598 1104 3 0.2784469606980622 1104 4 0.256437719363376 1104 5 0.17043721791800692 1104 6 0.2106063111276555 1104 7 0.2567049215390256 1104 8 0.3300996528455613 1104 9 0.2960901585693776 1104 10-11 0.36646514745442715 1104 12-13 0.3875856206992836 1104 14-15 0.3905909780956307 1104 16-17 0.48680731486907547 1104 18-19 0.46543254850100624 1104 20-21 0.4745035071586656 1104 22-23 0.419040589701666 1104 24-25 0.4850882906545877 1104 26-27 0.44100658165029216 1104 28-29 0.4707205612395029 1104 30-31 0.5517616259512081 1104 32-33 0.4940227989729351 1104 34-35 0.44091267419469204 1104 36-37 0.4901821246781921 1104 38-39 0.503730993444151 1104 40-41 0.4499501280021292 1104 42-43 0.3526801941821347 1104 44-45 0.56151174993186 1104 46-47 0.5760105881263868 1104 48-49 0.5239756743364126 1104 50-51 0.39695284976605905 1104 52-53 0.36566572416715815 1104 54-55 0.46373282220664436 1104 56-57 0.5211245514904732 1104 58-59 0.5638205663342859 1104 60-61 0.5178112628516587 1104 62-63 0.41357801873505906 1104 64-65 0.4933773614947441 1104 66-67 0.48651317757640555 1104 68-69 0.45744979126920526 1104 70-71 0.4719812620254302 1104 72-73 0.4738024221174584 1104 74-75 0.431025942910054 1104 76-77 0.3502354278873554 1104 78-79 0.4155219195846023 1104 80-81 0.37575845790260587 1104 82-83 0.5553340231786059 1104 84-85 0.4947970784953384 1104 86-87 0.42580102676751963 1104 88-89 0.43471209853201387 1104 90-91 0.54139212563895 1104 92-93 0.5635265882330778 1104 94-95 0.5936283751539655 1104 96-97 0.4877723200709809 1104 98-99 0.6999388412299119 1104 100-101 0.8106012452274278 1105 1 0.2625085002200791 1105 2 0.15713010780345726 1105 3 0.2696637262726398 1105 4 0.22425811387842742 1105 5 0.23010411768176908 1105 6 0.19629028205515908 1105 7 0.2172612186400471 1105 8 0.2893394148725932 1105 9 0.21906304124385656 1105 10-11 0.39946692670212514 1105 12-13 0.32760154193972824 1105 14-15 0.4395738345740554 1105 16-17 0.34688343279447764 1105 18-19 0.4396888892809869 1105 20-21 0.4404570066163629 1105 22-23 0.3413234932315561 1105 24-25 0.4636241883831502 1105 26-27 0.3848673465518786 1105 28-29 0.4904797241637979 1105 30-31 0.527121077652474 1105 32-33 0.5149877580240627 1105 34-35 0.4713208969642082 1105 36-37 0.5796275452892488 1105 38-39 0.6963389925461598 1105 40-41 0.7381774327784854 1105 42-43 0.6330950549964953 1105 44-45 0.6619348607977784 1105 46-47 0.6002162343781166 1105 48-49 0.7313602129394425 1105 50-51 0.539199984616026 1105 52-53 0.5722884291496726 1105 54-55 0.7796624184109149 1105 56-57 0.7107979055730951 1105 58-59 0.5396984147319088 1105 60-61 0.7121883795542701 1105 62-63 0.7379218244073371 1105 64-65 0.7653591998040454 1105 66-67 0.7905498512955447 1105 68-69 0.8669336634628664 1105 70-71 0.7033906781329406 1105 72-73 0.7008385306904472 1105 74-75 0.7085516497611621 1105 76-77 0.8024781872905073 1105 78-79 0.7278118537792402 1105 80-81 0.7168790882286942 1105 82-83 0.7206259958507495 1105 84-85 0.8071723949350265 1105 86-87 0.7177991185341135 1105 88-89 0.6645081236833441 1105 90-91 0.6765471859621073 1105 92-93 0.667339003000265 1105 94-95 0.7168957353400707 1105 96-97 0.737222809505667 1105 98-99 0.7398144260227824 1105 100-101 1.1128726394571977 1106 1 0.15230009329673067 1106 2 0.18866032177852432 1106 3 0.14650635142344726 1106 4 0.13705230590743156 1106 5 0.14949808902575512 1106 6 0.1681745638109362 1106 7 0.23718724883142528 1106 8 0.09590926420721502 1106 9 0.17691545825982757 1106 10-11 0.15742588424479464 1106 12-13 0.17266403049126922 1106 14-15 0.24630104709683565 1106 16-17 0.2248277824660505 1106 18-19 0.21560577430856398 1106 20-21 0.25952972869463053 1106 22-23 0.3638232709431506 1106 24-25 0.29818646484967815 1106 26-27 0.26378071577851614 1106 28-29 0.21989320587080385 1106 30-31 0.1655497876946228 1106 32-33 0.28725170352429075 1106 34-35 0.19313575914296166 1106 36-37 0.3284117005714293 1106 38-39 0.4574591827958798 1106 40-41 0.42767137803090804 1106 42-43 0.40865797913614443 1106 44-45 0.3653013033620951 1106 46-47 0.39321233959365287 1106 48-49 0.27680076184362434 1106 50-51 0.32809632630756624 1106 52-53 0.45159730168828105 1106 54-55 0.4285363150465642 1106 56-57 0.392528033353706 1106 58-59 0.5606572964778778 1106 60-61 0.6788747566030295 1106 62-63 0.5631646289436674 1106 64-65 0.49615044450738566 1106 66-67 0.43843474671310645 1106 68-69 0.4497359137503807 1106 70-71 0.5274192170494487 1106 72-73 0.5509697415865134 1106 74-75 0.4417679031009527 1106 76-77 0.30613616778451913 1106 78-79 0.4487617346666646 1106 80-81 0.47554013703156883 1106 82-83 0.6313613477733888 1106 84-85 0.5847326958285031 1106 86-87 0.6000947334518791 1106 88-89 0.7134089014255451 1106 90-91 0.789164385400241 1106 92-93 0.769491277120018 1106 94-95 0.7733976834611092 1106 96-97 0.9188120938924698 1106 98-99 1.1074019578537566 1106 100-101 1.2406757813277345 1107 1 0.10678378894890628 1107 2 0.1560226580622448 1107 3 0.2232563028318566 1107 4 0.21183056004893075 1107 5 0.18724581269658813 1107 6 0.12222841076984992 1107 7 0.0850829525871859 1107 8 0.12863429934267145 1107 9 0.1421649947708019 1107 10-11 0.14245219846045387 1107 12-13 0.23627299339719343 1107 14-15 0.28933777860212473 1107 16-17 0.2345066200804311 1107 18-19 0.1822067644556853 1107 20-21 0.12702653286304155 1107 22-23 0.2607193895963391 1107 24-25 0.2936284334071786 1107 26-27 0.28891144584849116 1107 28-29 0.38758582480805615 1107 30-31 0.41411213070640684 1107 32-33 0.3919464922630027 1107 34-35 0.40960942997604377 1107 36-37 0.4759673099099828 1107 38-39 0.5267783585329795 1107 40-41 0.3402934176816501 1107 42-43 0.4492546184669237 1107 44-45 0.2752912206068814 1107 46-47 0.5208855779615789 1107 48-49 0.6339022360377484 1107 50-51 0.6372951350660117 1107 52-53 0.5856257595406902 1107 54-55 0.6665159701210186 1107 56-57 0.6779653202982701 1107 58-59 0.645381875599945 1107 60-61 0.7082137988253905 1107 62-63 0.6402505164503935 1107 64-65 0.5383111256867465 1107 66-67 0.5559016483637222 1107 68-69 0.6237851538526442 1107 70-71 0.46651035805476937 1107 72-73 0.5003008596416549 1107 74-75 0.5850682439896531 1107 76-77 0.6517667558175297 1107 78-79 0.8076926355547691 1107 80-81 0.6688066506970181 1107 82-83 0.5762239083259857 1107 84-85 0.6851960446862257 1107 86-87 0.7057996666197504 1107 88-89 0.8405473986745129 1107 90-91 0.6897303372721382 1107 92-93 0.7473019736244098 1107 94-95 0.6890679110192721 1107 96-97 0.7664765203621791 1107 98-99 0.8194138459734859 1107 100-101 0.9098717026243044 1108 1 0.36030859790339065 1108 2 0.36610868631848703 1108 3 0.38770782695905126 1108 4 0.3952354552050039 1108 5 0.3013100459724072 1108 6 0.29323195820768433 1108 7 0.3905494637725937 1108 8 0.32004644729509124 1108 9 0.4219176370534896 1108 10-11 0.39931597068490277 1108 12-13 0.4718532219199787 1108 14-15 0.5795240190883746 1108 16-17 0.5677728290980824 1108 18-19 0.5485561533796641 1108 20-21 0.5341053757281102 1108 22-23 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-2.1656761672075007 2316 48-49 -2.3157897929477613 2316 50-51 -1.9443677574460665 2316 52-53 -2.0024365894931293 2316 54-55 -2.0244607810867095 2316 56-57 -1.7649060565133254 2316 58-59 -1.4968660108734895 2316 60-61 -1.4921213461021452 2316 62-63 -1.8225061260616826 2316 64-65 -1.9747927390475546 2316 66-67 -2.0067719627473863 2316 68-69 -2.2695428956642587 2316 70-71 -2.103988585181945 2316 72-73 -2.0928966041264587 2316 74-75 -1.9495875531117939 2316 76-77 -2.0168790775158065 2316 78-79 -2.1246390371505406 2316 80-81 -1.9946927454382468 2316 82-83 -1.932794811480779 2316 84-85 -2.0639034420287494 2316 86-87 -2.1733685097087516 2316 88-89 -2.213237323547709 2316 90-91 -2.3934422170756875 2316 92-93 -2.400795097631626 2316 94-95 -2.4599870406715496 2316 96-97 -2.755658141473571 2316 98-99 -2.7925147470216842 2316 100-101 -2.6946633215302818 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7991.0 3 2072.0 4 2225.0 5 2478.0 6 2880.0 7 3630.0 8 4435.0 9 5713.0 10 6995.0 11 9712.0 12 13066.0 13 19148.0 14 17146.0 15 17792.0 16 22763.0 17 30155.0 18 25965.0 19 18560.0 20 18142.0 21 19230.0 22 21322.0 23 23751.0 24 27163.0 25 30659.0 26 36076.0 27 42102.0 28 49214.0 29 58790.0 30 70253.0 31 85527.0 32 105592.0 33 134795.0 34 179126.0 35 254492.0 36 388149.0 37 595477.0 38 544060.0 39 83978.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21382443835779 19.766143563494914 14.397786183303673 22.622245814843627 2 14.953538414422837 21.385089847223455 40.34283384778223 23.31853789057148 3 19.14446311322494 25.72759827500916 30.91895961867775 24.20897899308815 4 12.470705191285278 17.03750474392861 38.66256477771546 31.829225287070656 5 12.90219538083274 38.129660481801444 34.849353074742375 14.11879106262344 6 30.15286881641307 37.17886571324794 17.954549574439373 14.713715895899618 7 26.672481014408973 34.056635506865426 21.961952949438672 17.308930529286922 8 25.07373759821888 34.97921650486806 20.824551850640827 19.12249404627223 9 26.059534416530088 17.356315510653257 21.79850096383876 34.7856491089779 10-11 24.256535621330936 26.855056296329614 28.199158651949734 20.68924943038971 12-13 25.67205986346053 24.856609716751002 28.25621536934359 21.21511505044488 14-15 22.595601403871555 25.862389032211503 27.453376324521926 24.088633239395012 16-17 21.084161814394506 29.084393447796042 28.057317517359913 21.77412722044954 18-19 20.90623234283677 28.071779862197328 30.322882474928715 20.699105320037184 20-21 22.04533200293565 27.116007141710856 29.80532861874773 21.033332236605766 22-23 21.638540016571262 27.040183207285985 29.768464069159407 21.552812706983353 24-25 21.67136063204567 27.14233637740053 29.981383654388477 21.20491933616533 26-27 21.585866100768037 26.993300857629247 30.386543486666945 21.03428955493577 28-29 21.089885463436715 27.491830929539645 30.36644379904506 21.051839807978574 30-31 21.372574926964706 27.224850315637035 29.978240091653184 21.424334665745075 32-33 21.24478905153981 27.41550218241648 29.95655983141649 21.383148934627222 34-35 21.807269875357214 27.14316248666225 29.755595124693272 21.293972513287265 36-37 21.90015147366476 26.849887305212018 29.99185306380103 21.258108157322194 38-39 21.506139214870245 27.251816615281093 30.070783479550865 21.171260690297792 40-41 21.5601464985156 27.350805027274667 29.94685283006658 21.14219564414316 42-43 21.379501595182003 27.49063752491813 29.39352739624995 21.73633348364991 44-45 21.467601315855138 27.46132753544217 29.265081553075927 21.805989595626766 46-47 21.827821683729464 27.664981123194384 28.821292942330405 21.68590425074575 48-49 21.811390224992643 27.617773194388285 28.849471064787103 21.721365515831973 50-51 21.399969009328732 27.899444545329928 28.968808176006778 21.731778269334562 52-53 21.70540256820022 28.2052341268008 28.44433998117638 21.645023323822603 54-55 21.319846248855455 27.61547310138933 28.844929268842463 22.219751380912744 56-57 21.70242066388321 28.000428137752483 28.42086278185033 21.87628841651398 58-59 22.02652355566177 27.56119358865484 28.53032415525706 21.88195870042633 60-61 21.70033941156533 27.53711324298081 28.99782283349696 21.764724511956896 62-63 21.617968027088523 27.55531520025678 28.994536206335887 21.832180566318815 64-65 21.453011687386685 27.76671630838298 28.90550807217474 21.8747639320556 66-67 21.45187450652908 27.664287663235378 28.499490888094204 22.384346942141345 68-69 21.223677894710566 27.738195509569515 28.78716684471379 22.250959751006132 70-71 21.53769636978057 27.619735244111837 28.590252729195686 22.252315656911907 72-73 21.55986942375593 27.271973021347982 28.813704232463216 22.354453322432867 74-75 21.209298356765512 27.991580368876573 28.429071336339877 22.370049938018038 76-77 21.382310827740696 27.44815398707131 28.655748948108723 22.513786237079277 78-79 21.459278035466806 27.545601069067665 28.61192122368333 22.383199671782204 80-81 21.457011268100416 27.676902905809904 28.453917369960703 22.412168456128985 82-83 21.438448007698195 27.467142251687605 28.698981183057636 22.395428557556567 84-85 21.41526870006344 27.34500057403917 28.478276958641025 22.761453767256366 86-87 21.439336799220733 27.285282274039325 28.678571705532867 22.596809221207074 88-89 21.579628233709702 27.080726052453528 28.520594196186437 22.819051517650326 90-91 21.381469954771166 26.968174232599594 28.83013920053068 22.820216612098555 92-93 21.72096958246397 27.135196225223225 28.587819528813935 22.55601466349887 94-95 21.471906881597665 26.87230538707564 28.63637091871281 23.019416812613887 96-97 21.263125198560047 26.7098423053808 28.826382804210855 23.200649691848298 98-99 21.46900343858183 26.797043065830184 28.364784105930784 23.36916938965721 100-101 21.709367327288707 26.085502928601667 29.09170946342717 23.113420280682455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3207.0 1 1959.0 2 803.0 3 906.0 4 840.5 5 906.5 6 1459.0 7 2642.5 8 4145.5 9 4945.0 10 4634.5 11 4287.0 12 4569.0 13 4947.5 14 5209.0 15 5512.5 16 5839.5 17 6081.5 18 6283.5 19 6602.5 20 7177.0 21 8123.0 22 9392.0 23 11132.0 24 13096.0 25 15880.5 26 20762.0 27 26494.0 28 30583.0 29 34619.5 30 39433.5 31 44874.0 32 51170.5 33 58154.5 34 65995.5 35 72583.5 36 81038.5 37 88535.0 38 88202.5 39 87335.0 40 89745.0 41 92822.0 42 95340.5 43 98638.0 44 103342.0 45 106142.0 46 107840.0 47 115970.5 48 139294.5 49 138128.5 50 111444.5 51 97558.0 52 89963.5 53 86899.5 54 84494.0 55 78335.5 56 71341.0 57 63424.5 58 55757.0 59 49261.0 60 41834.5 61 33416.5 62 25983.5 63 20267.5 64 15278.5 65 11302.0 66 8128.0 67 6043.0 68 4407.0 69 2939.0 70 2108.5 71 1440.5 72 927.5 73 615.0 74 432.5 75 305.5 76 203.5 77 126.5 78 88.0 79 58.0 80 39.0 81 30.0 82 27.0 83 22.0 84 16.0 85 15.5 86 13.0 87 8.0 88 10.0 89 10.0 90 7.5 91 6.5 92 6.0 93 5.5 94 2.5 95 1.0 96 2.0 97 2.0 98 1.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3299970174120732 2 0.09021573605338194 3 0.10044846178647288 4 0.10544739993149105 5 0.11651887367549107 6 0.05834331163883645 7 0.06022210718327281 8 0.06129570463723645 9 0.06438229731738192 10-11 0.06179895344378191 12-13 0.05997048278000009 14-15 0.057974262514036444 16-17 0.05998725774021827 18-19 0.06797213880407282 20-21 0.06961608490545464 22-23 0.08674331928821831 24-25 0.09640569637389106 26-27 0.09107125902450922 28-29 0.08830339058850922 30-31 0.08218053010887284 32-33 0.07275300246625466 34-35 0.32687687481149136 36-37 0.506637348509528 38-39 0.4808877845746189 40-41 0.4622172538517825 42-43 0.49219410776167344 44-45 0.5018061599666916 46-47 0.4973440205486553 48-49 0.5145215798120735 50-51 0.510780763683419 52-53 0.5102942898370917 54-55 0.5009506369955644 56-57 0.47969676239912795 58-59 0.4629385771411644 60-61 0.4212192510785461 62-63 0.49162375911425527 64-65 0.5161655259134552 66-67 0.5255930535560736 68-69 0.5388788220488735 70-71 0.5360438537720008 72-73 0.5241168570568735 74-75 0.5253078792323643 76-77 0.5000112392233462 78-79 0.4800993614443643 80-81 0.48553444855505523 82-83 0.49459292707287345 84-85 0.4967904468614553 86-87 0.49566652452683707 88-89 0.5025610331765098 90-91 0.5159474514306189 92-93 0.5228251851200735 94-95 0.5302732674569463 96-97 0.5211980139789099 98-99 0.5234626336083643 100-101 0.5021919840517098 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2980633.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.33033353920153 #Duplication Level Percentage of deduplicated Percentage of total 1 68.5129418211201 29.001756790399234 2 15.018643609760208 12.714883866150945 3 6.078206035772915 7.718774664427662 4 3.167809153895699 5.36377672291763 5 1.8905353919543577 4.001349685454653 6 1.1383971420949157 2.8913238434970947 7 0.8092256926078539 2.3978355433607277 8 0.5948975664858236 2.0145769928803388 9 0.44213812707955424 1.6844268950707866 >10 2.098766352640717 15.793238411442307 >50 0.1493758641605094 4.424204530387678 >100 0.09221201137219415 7.114385156214727 >500 0.004142604824138254 1.207372478090081 >1k 0.0023102988441619766 2.094683168306311 >5k 1.593309547697915E-4 0.41512597282219577 >10k+ 2.3899643215468725E-4 1.1622852785775628 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13782 0.4623850034539643 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10454 0.35073086824174593 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10230 0.3432156860640005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6783 0.22756911031985488 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5527 0.18543041025178209 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4825 0.16187836610545478 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4420 0.1482906483287275 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3815 0.12799294646472748 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3759 0.12611415092029107 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3020 0.10132075971781833 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.709984087272737E-5 0.0 0.0 0.0 0.0 7 6.709984087272737E-5 0.0 0.0 0.0 0.0 8 6.709984087272737E-5 0.0 0.0 0.0 0.0 9 6.709984087272737E-5 0.0 0.0 0.0 0.0 10-11 6.709984087272737E-5 0.0 0.0 5.0324880654545525E-5 0.0 12-13 6.709984087272737E-5 1.677496021818184E-5 0.0 6.709984087272737E-5 0.0 14-15 6.709984087272737E-5 3.354992043636368E-5 0.0 6.709984087272737E-5 0.0 16-17 1.3419968174545473E-4 3.354992043636368E-5 0.0 2.1807448283636395E-4 0.0 18-19 1.3419968174545473E-4 3.354992043636368E-5 0.0 3.5227416458181874E-4 0.0 20-21 1.3419968174545473E-4 3.354992043636368E-5 0.0 5.032488065454553E-4 0.0 22-23 1.3419968174545473E-4 3.354992043636368E-5 0.0 9.729476926545468E-4 0.0 24-25 1.3419968174545473E-4 3.354992043636368E-5 0.0 0.0015600713002909112 0.0 26-27 1.3419968174545473E-4 3.354992043636368E-5 0.0 0.002247844669236367 0.0 28-29 1.3419968174545473E-4 3.354992043636368E-5 0.0 0.0067435340077091005 0.0 30-31 1.3419968174545473E-4 3.354992043636368E-5 0.0 0.0193079792111273 0.0 32-33 1.3419968174545473E-4 3.354992043636368E-5 0.0 0.0431451976811637 0.0 34-35 1.3419968174545473E-4 3.354992043636368E-5 0.0 0.07884231302545466 0.0 36-37 1.3419968174545473E-4 3.354992043636368E-5 0.0 0.1388631206861093 0.0 38-39 1.3419968174545473E-4 3.354992043636368E-5 0.0 0.2273342608768003 0.0 40-41 1.3419968174545473E-4 3.354992043636368E-5 0.0 0.323521882767855 0.0 42-43 1.3419968174545473E-4 3.354992043636368E-5 0.0 0.4244064935200006 0.0 44-45 1.3419968174545473E-4 3.354992043636368E-5 0.0 0.5464946539879281 0.0 46-47 1.3419968174545473E-4 3.354992043636368E-5 0.0 0.6944665780725101 0.0 48-49 1.3419968174545473E-4 3.354992043636368E-5 0.0 0.8530067270945467 0.0 50-51 1.6774960218181843E-4 3.354992043636368E-5 0.0 1.0245474702856743 0.0 52-53 1.6774960218181843E-4 3.354992043636368E-5 0.0 1.19352164456342 0.0 54-55 1.6774960218181843E-4 3.354992043636368E-5 0.0 1.3853265396981111 0.0 56-57 1.6774960218181843E-4 3.354992043636368E-5 0.0 1.6131304994610205 0.0 58-59 1.6774960218181843E-4 3.354992043636368E-5 0.0 1.866684023158839 0.0 60-61 1.6774960218181843E-4 3.354992043636368E-5 0.0 2.149744701880439 0.0 62-63 1.6774960218181843E-4 3.354992043636368E-5 0.0 2.441041886069167 0.0 64-65 1.6774960218181843E-4 3.354992043636368E-5 0.0 2.7390155044247315 0.0 66-67 1.6774960218181843E-4 3.354992043636368E-5 0.0 3.0869449543100407 0.0 68-69 1.6774960218181843E-4 3.354992043636368E-5 0.0 3.483806963151787 0.0 70-71 1.6774960218181843E-4 3.354992043636368E-5 0.0 3.913732418583569 0.0 72-73 1.6774960218181843E-4 3.354992043636368E-5 0.0 4.355366796247642 0.0 74-75 1.6774960218181843E-4 3.354992043636368E-5 0.0 4.81708080129288 0.0 76-77 1.6774960218181843E-4 3.354992043636368E-5 0.0 5.305601192766771 0.0 78-79 1.6774960218181843E-4 3.354992043636368E-5 0.0 5.868602407609391 0.0 80-81 1.6774960218181843E-4 3.354992043636368E-5 0.0 6.480536181408446 0.0 82-83 1.6774960218181843E-4 3.354992043636368E-5 0.0 7.099599313300228 0.0 84-85 1.6774960218181843E-4 3.354992043636368E-5 0.0 7.739077571777539 0.0 86-87 1.6774960218181843E-4 3.354992043636368E-5 0.0 8.421348753771431 0.0 88-89 2.012995226181821E-4 3.354992043636368E-5 0.0 9.14359466596525 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGATT 30 0.0039254352 31.710764 68-69 GTACATG 12040 0.0 24.401194 1 TACATGG 12150 0.0 23.275845 2 GAGTACT 10675 0.0 22.510162 12-13 ACATGGG 11995 0.0 21.957449 3 CATGGGG 5115 0.0 20.559603 4 GTACTTT 11820 0.0 20.209944 14-15 AGTACTT 11215 0.0 20.180836 12-13 ATGGGGA 3060 0.0 18.885935 5 GTATCAA 31655 0.0 17.407518 1 GTATAGG 1125 0.0 17.2973 1 AGTACAT 9255 0.0 17.095327 2 CATGGGA 7040 0.0 17.091038 4 GTGTAGC 1580 0.0 16.82201 1 ACTTTTT 14545 0.0 16.604061 16-17 TCAACGC 34795 0.0 15.724343 4 CAACGCA 34730 0.0 15.71259 5 ATCAACG 34925 0.0 15.665814 3 AGAGTAC 22895 0.0 15.66435 10-11 TATCAAC 35130 0.0 15.628333 2 >>END_MODULE