FastQCFastQC Report
Fri 27 May 2016
SRR939009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980633
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134680.4518503284369461No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110930.37216926740058237No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT108830.36512378410894597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69080.23176285037440036No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52450.17596933268872753No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA48690.16335456260465478No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42110.14127871495752747No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA39950.1340319321432729No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36290.12175266126356382No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30260.10152205924043652No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG702.0662734E-433.89455
GTACATG113050.023.3873211
TACATGG112600.022.6304472
GAGTACT117400.022.2527112-13
ACATGGG114350.021.6201553
ACCGGAC1100.00289525921.5692273
CATGGGG48000.020.4638044
AGTACTT123900.020.18519612-13
GTACTTT128250.020.14810614-15
CTCCGAC5700.018.731894-95
GTGTACG2854.1385647E-818.3867911
TACCCGA1600.001199676317.7946112
GTACGCT1600.001199676317.7946113
AGTACAT87700.017.5849482
TTGCGTT7300.017.550854
CTACACT11700.017.0341594
GTATCAA312050.016.9150661
ACTTTTT155150.016.65411816-17
CTTTGCG6850.016.6256242
CATGGGA68400.016.5805554
Produced by FastQC (version 0.11.5)