##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939009_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2980633 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.181867744200645 33.0 31.0 34.0 30.0 34.0 2 32.416502132265194 34.0 31.0 34.0 31.0 34.0 3 32.50466360669026 34.0 31.0 34.0 30.0 34.0 4 36.05460685699984 37.0 35.0 37.0 35.0 37.0 5 35.981398246614056 37.0 35.0 37.0 35.0 37.0 6 36.02666279276919 37.0 35.0 37.0 35.0 37.0 7 35.96372918101625 37.0 35.0 37.0 35.0 37.0 8 35.94156576807678 37.0 35.0 37.0 35.0 37.0 9 37.61165598045784 39.0 37.0 39.0 35.0 39.0 10-11 37.528861486804985 39.0 37.0 39.0 35.0 39.0 12-13 37.443701723761365 39.0 37.0 39.0 34.0 39.0 14-15 38.746438592070874 40.0 38.0 41.0 34.5 41.0 16-17 38.8364961402494 40.0 38.0 41.0 35.0 41.0 18-19 38.89418908668058 40.0 38.0 41.0 35.0 41.0 20-21 38.87272653157903 40.0 38.0 41.0 34.5 41.0 22-23 38.794669622190995 40.0 38.0 41.0 34.0 41.0 24-25 38.72132379263063 40.0 38.0 41.0 34.0 41.0 26-27 38.559230203785575 40.0 38.0 41.0 34.0 41.0 28-29 38.40243481837582 40.0 38.0 41.0 34.0 41.0 30-31 38.20521949532197 40.0 38.0 41.0 34.0 41.0 32-33 38.0890483665718 40.0 37.0 41.0 33.0 41.0 34-35 37.979725112081894 40.0 37.0 41.0 33.0 41.0 36-37 37.84303652948887 40.0 37.0 41.0 33.0 41.0 38-39 37.695330824022946 40.0 37.0 41.0 33.0 41.0 40-41 37.526597202674736 40.0 37.0 41.0 32.5 41.0 42-43 37.33728171163642 40.0 37.0 41.0 32.0 41.0 44-45 37.07022400946376 40.0 36.5 41.0 31.0 41.0 46-47 36.731429364165265 40.0 36.0 41.0 30.5 41.0 48-49 36.382452821263136 39.5 36.0 41.0 30.0 41.0 50-51 36.12334141774583 39.0 35.5 41.0 29.5 41.0 52-53 35.87163766891127 39.0 35.0 40.0 28.5 41.0 54-55 35.577487734987834 39.0 35.0 40.0 28.0 41.0 56-57 35.46492942941986 38.5 35.0 40.0 28.0 41.0 58-59 35.201583522694676 38.0 34.5 40.0 27.5 41.0 60-61 34.848323325951235 38.0 34.0 40.0 26.5 41.0 62-63 34.448006010803745 37.5 34.0 40.0 26.0 41.0 64-65 34.0429940552896 37.0 33.5 39.5 26.0 41.0 66-67 33.59395537793482 36.0 33.0 39.0 24.5 40.5 68-69 33.10650321592762 36.0 32.5 39.0 23.0 40.0 70-71 32.58066726094759 35.0 32.0 38.0 22.0 40.0 72-73 32.07213316768619 35.0 31.0 37.5 20.5 39.5 74-75 31.545647518496907 35.0 31.0 37.0 19.5 39.0 76-77 29.62435110260136 33.0 28.5 35.0 16.0 37.5 78-79 30.58006285913093 34.0 30.0 36.0 18.0 38.0 80-81 30.55569941015885 34.0 30.5 35.5 17.0 37.0 82-83 30.143488312717466 34.0 30.0 35.0 10.0 37.0 84-85 29.694381529024206 34.0 30.0 35.0 7.0 36.0 86-87 29.007331496363356 34.0 29.0 35.0 2.0 36.0 88-89 28.313514612500096 33.0 28.5 35.0 2.0 35.5 90-91 27.734865379266754 33.0 26.5 35.0 2.0 35.0 92-93 27.346613622005798 33.0 26.0 35.0 2.0 35.0 94-95 26.808550063023525 32.5 25.5 34.0 2.0 35.0 96-97 25.968769721062607 32.0 24.5 34.0 2.0 35.0 98-99 24.77087098612946 32.0 11.0 34.0 2.0 35.0 100-101 19.192928314220502 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04772921443022682 1101 2 0.0773996282860594 1101 3 0.10009202206668277 1101 4 0.10536918671368056 1101 5 0.11856443539670636 1101 6 0.19490824246624783 1101 7 0.13638974987751595 1101 8 0.12694333565779203 1101 9 0.15289494800789782 1101 10-11 0.23035537084073354 1101 12-13 0.20124818150502222 1101 14-15 0.2512172557113317 1101 16-17 0.23848806626838126 1101 18-19 0.2478765975550914 1101 20-21 0.24831579766293288 1101 22-23 0.21526429808891834 1101 24-25 0.11388080500171327 1101 26-27 0.21066056443316228 1101 28-29 0.27553720939762627 1101 30-31 0.2596111024554091 1101 32-33 0.2491208615493008 1101 34-35 0.32207353415468276 1101 36-37 0.33464150675261806 1101 38-39 0.2779188772526098 1101 40-41 0.35144637006461465 1101 42-43 0.33487214403231036 1101 44-45 0.3560945989057771 1101 46-47 0.36082572874239105 1101 48-49 0.3973234936411316 1101 50-51 0.30641022443624166 1101 52-53 0.17178401519051079 1101 54-55 0.30592862341443805 1101 56-57 0.39325251202318867 1101 58-59 0.39476941820528566 1101 60-61 0.33550603546003543 1101 62-63 0.35043842544545356 1101 64-65 0.33541314248699905 1101 66-67 0.3121026707071408 1101 68-69 0.3678333438504424 1101 70-71 0.39225879367383953 1101 72-73 0.5816189229222175 1101 74-75 0.5444023105720497 1101 76-77 0.22112967964809727 1101 78-79 0.5664319343649744 1101 80-81 0.6040687206596225 1101 82-83 0.5672116192773586 1101 84-85 0.5681878546456822 1101 86-87 0.9022531282801403 1101 88-89 0.7993526867026368 1101 90-91 0.7545693564943896 1101 92-93 0.6408287573821667 1101 94-95 0.4868431356737979 1101 96-97 0.544055957299534 1101 98-99 0.7639822489785928 1101 100-101 0.8910347585022471 1102 1 0.14718219444835512 1102 2 0.1796451614956709 1102 3 0.12463832995153723 1102 4 0.08480172091591953 1102 5 0.09380638654253914 1102 6 0.08770716137350121 1102 7 0.14988398836528205 1102 8 0.16352327394271526 1102 9 0.188821860959429 1102 10-11 0.20596591311339552 1102 12-13 0.21228298794410705 1102 14-15 0.34777558832538347 1102 16-17 0.3131583167970575 1102 18-19 0.2745388315976527 1102 20-21 0.29092050829259364 1102 22-23 0.2526601808734412 1102 24-25 0.2841655898620985 1102 26-27 0.22435656374265278 1102 28-29 0.2668121327932482 1102 30-31 0.23643800893333378 1102 32-33 0.24571304574507025 1102 34-35 0.3426701311940832 1102 36-37 0.34634269034417997 1102 38-39 0.37961172607226246 1102 40-41 0.2943000449168025 1102 42-43 0.3704574392288933 1102 44-45 0.37881669764988857 1102 46-47 0.4197838552798743 1102 48-49 0.37767406655554936 1102 50-51 0.4289998839243978 1102 52-53 0.30402056626729035 1102 54-55 0.27016813616744884 1102 56-57 0.2015263241158891 1102 58-59 0.12379546369318462 1102 60-61 0.1865992662226219 1102 62-63 0.39939603948887026 1102 64-65 0.36524043198680545 1102 66-67 0.222889187258005 1102 68-69 0.25689618101760203 1102 70-71 0.36035441967185733 1102 72-73 0.3587467173635446 1102 74-75 0.4506305970076845 1102 76-77 0.24409683147581873 1102 78-79 0.44062381214322954 1102 80-81 0.4415104581763494 1102 82-83 0.41967213026091343 1102 84-85 0.5692926839581851 1102 86-87 0.6130643254662793 1102 88-89 0.4087872978545093 1102 90-91 0.4585882540387338 1102 92-93 0.6003664068146435 1102 94-95 0.8731747840705033 1102 96-97 0.9965288760341586 1102 98-99 1.289680050109311 1102 100-101 1.2249077840514282 1103 1 0.08855325480308096 1103 2 0.1255850938677554 1103 3 0.07691545555232437 1103 4 0.12415324786075388 1103 5 0.11010912781258497 1103 6 0.11268828722948854 1103 7 0.11444632089047957 1103 8 0.12935974410562068 1103 9 0.1491080036160568 1103 10-11 0.11879674229973602 1103 12-13 0.12776138103669865 1103 14-15 0.2250334410326218 1103 16-17 0.1359860623204341 1103 18-19 0.18033917505390207 1103 20-21 0.13157727533781127 1103 22-23 0.13339685732275797 1103 24-25 0.11424326626888615 1103 26-27 0.14208230913842357 1103 28-29 0.14504120641835527 1103 30-31 0.2998155237136473 1103 32-33 0.23488673040331065 1103 34-35 0.2351303094085324 1103 36-37 0.21990971748041233 1103 38-39 0.31978547453042694 1103 40-41 0.4636734233354147 1103 42-43 0.3538116404538485 1103 44-45 0.3536744769872229 1103 46-47 0.42435644895942914 1103 48-49 0.32784034510601145 1103 50-51 0.4504355948682175 1103 52-53 0.41941375987878615 1103 54-55 0.45606968225225586 1103 56-57 0.46910267135760364 1103 58-59 0.32469025761942305 1103 60-61 0.3516579089855867 1103 62-63 0.32389055801211697 1103 64-65 0.41927617589391275 1103 66-67 0.3708774959220378 1103 68-69 0.38783595388986924 1103 70-71 0.37455548513057835 1103 72-73 0.45089440886965093 1103 74-75 0.4508473838308724 1103 76-77 0.269043266123429 1103 78-79 0.46821089903872704 1103 80-81 0.48568712016435356 1103 82-83 0.3541660905348323 1103 84-85 0.5046059415822732 1103 86-87 0.5996947856763946 1103 88-89 0.5362479811516891 1103 90-91 0.5545057235211068 1103 92-93 0.6665158953519281 1103 94-95 0.7590844380622492 1103 96-97 0.5300132025196334 1103 98-99 0.7885712606065276 1103 100-101 1.2017490764252123 1104 1 9.128887210749781E-4 1104 2 0.039296322458810096 1104 3 0.04706728906636215 1104 4 0.03188765628757295 1104 5 0.025623770603395712 1104 6 0.06627400581701437 1104 7 0.05818938034750687 1104 8 0.07610068021694616 1104 9 0.1412325883426533 1104 10-11 0.05492996569203257 1104 12-13 0.06669239509849234 1104 14-15 0.11018698368152258 1104 16-17 0.14082451108026106 1104 18-19 0.11328905425529001 1104 20-21 0.1320190487062618 1104 22-23 0.05230497644752319 1104 24-25 0.022552946051462186 1104 26-27 0.025884257114263676 1104 28-29 0.10164508717200249 1104 30-31 0.016973408878854457 1104 32-33 0.06792563402226648 1104 34-35 0.1051846694283256 1104 36-37 0.11521953971235632 1104 38-39 0.027148870605877562 1104 40-41 0.11183577491951979 1104 42-43 -0.06001280061267522 1104 44-45 0.005237646328616563 1104 46-47 0.09784304030051771 1104 48-49 0.12239544326084228 1104 50-51 0.2258109025962085 1104 52-53 0.12539065732116228 1104 54-55 0.2999168997626569 1104 56-57 0.13770766697722792 1104 58-59 0.17377319792902313 1104 60-61 0.2238589269447644 1104 62-63 0.29104277611369866 1104 64-65 0.2723536232732897 1104 66-67 0.18979027749460187 1104 68-69 0.25886028336676503 1104 70-71 0.31888102856977696 1104 72-73 0.30699027600894624 1104 74-75 0.2682346305163499 1104 76-77 0.2331162079296405 1104 78-79 0.45650113564120787 1104 80-81 0.4123656206902311 1104 82-83 0.34282616743688266 1104 84-85 0.3861182670592562 1104 86-87 0.4846410297140622 1104 88-89 0.6243655397497676 1104 90-91 0.5795341167978059 1104 92-93 0.4336778891382487 1104 94-95 0.6828359646275111 1104 96-97 0.654741537657408 1104 98-99 0.8464107286650275 1104 100-101 0.9678721300142534 1105 1 0.013655795545453486 1105 2 0.08326773821976019 1105 3 0.07893191775048791 1105 4 0.05856995392441888 1105 5 0.08199895516306555 1105 6 0.015562237526182798 1105 7 0.05962332387341718 1105 8 0.0539284778822946 1105 9 0.0671934091548323 1105 10-11 0.17310994089405796 1105 12-13 0.13951847945615015 1105 14-15 0.1145423590569905 1105 16-17 0.04737046642200937 1105 18-19 0.14566100574106144 1105 20-21 0.12574984006062806 1105 22-23 0.04295980478516981 1105 24-25 0.04268484620469337 1105 26-27 -0.009819678283655264 1105 28-29 0.09479317773683249 1105 30-31 0.13228480491063976 1105 32-33 0.11823496691197732 1105 34-35 0.02981001628976543 1105 36-37 0.07793845519550757 1105 38-39 0.1437508787308488 1105 40-41 0.13903558837473184 1105 42-43 0.18453302310073383 1105 44-45 0.001954047785993396 1105 46-47 0.028202336589743027 1105 48-49 0.13153851634464075 1105 50-51 -0.013800934742874915 1105 52-53 -0.09012337839994444 1105 54-55 0.031007815557394736 1105 56-57 0.06922091343165704 1105 58-59 0.08140319947558794 1105 60-61 8.936072370246961E-4 1105 62-63 0.10657320874937426 1105 64-65 -0.1306868151925329 1105 66-67 -0.11608107631546005 1105 68-69 -0.06413099707370407 1105 70-71 0.014706057006407036 1105 72-73 -0.0692228345511765 1105 74-75 -0.07374196153533674 1105 76-77 -0.17227774776244686 1105 78-79 0.02482706603397844 1105 80-81 0.010117217375967158 1105 82-83 -0.014074259499508912 1105 84-85 0.046255719260614114 1105 86-87 0.05737040625253442 1105 88-89 0.1699505352264623 1105 90-91 0.19927104384487393 1105 92-93 0.3152398796053859 1105 94-95 0.48414569177304045 1105 96-97 0.5605833568869016 1105 98-99 0.6655310537039725 1105 100-101 0.8133745620554578 1106 1 0.035739116529427406 1106 2 0.02503667779958363 1106 3 2.866429218428834E-4 1106 4 -0.04389042650701214 1106 5 -0.05129890110209345 1106 6 0.03917825531314634 1106 7 0.08433863679962172 1106 8 0.028219547913103327 1106 9 0.03143352047880654 1106 10-11 0.029057637767493816 1106 12-13 0.04296811619072827 1106 14-15 -0.03358541377834712 1106 16-17 -0.10893059614087974 1106 18-19 -0.03100191805847885 1106 20-21 0.04702992248468263 1106 22-23 0.059117013950547914 1106 24-25 0.1195674225346437 1106 26-27 0.08061313789912816 1106 28-29 0.02383282621504179 1106 30-31 0.014059074553529172 1106 32-33 0.09586576845725148 1106 34-35 0.013491496191250008 1106 36-37 0.03417417954233315 1106 38-39 0.0534789483394249 1106 40-41 0.16120776398791747 1106 42-43 0.274283851155424 1106 44-45 0.21026999079658992 1106 46-47 0.25017516930203954 1106 48-49 0.22081342855894803 1106 50-51 0.14726390204884154 1106 52-53 0.17909819188424336 1106 54-55 0.1751326357224272 1106 56-57 0.11041964072726529 1106 58-59 0.15204511981423252 1106 60-61 0.33337438961448385 1106 62-63 0.35622835804215214 1106 64-65 0.27032250780200684 1106 66-67 0.4456562497226102 1106 68-69 0.31724491473391225 1106 70-71 0.40801654848486635 1106 72-73 0.3517161627070138 1106 74-75 0.33995189120624403 1106 76-77 0.13105294645275833 1106 78-79 0.436276777593509 1106 80-81 0.4287960395340633 1106 82-83 0.3047144071441572 1106 84-85 0.40868907525197784 1106 86-87 0.7154709724441908 1106 88-89 0.8066595468228712 1106 90-91 0.8649249823339069 1106 92-93 0.9630709642222044 1106 94-95 0.9089763760277805 1106 96-97 0.8483777311195198 1106 98-99 0.8885558693503555 1106 100-101 1.1895502005090712 1107 1 -0.023242198134767023 1107 2 0.05534848630385625 1107 3 0.047500382381208794 1107 4 -0.03300918061860614 1107 5 0.011936981171693617 1107 6 0.001517903061483139 1107 7 0.026474461511057257 1107 8 -0.008951150609711078 1107 9 -0.012029321905018264 1107 10-11 0.06804631592670063 1107 12-13 0.1292826682510153 1107 14-15 0.11125864459136636 1107 16-17 0.07462530848503945 1107 18-19 -0.011330733619743683 1107 20-21 0.08658067453300333 1107 22-23 0.12563212780438704 1107 24-25 0.05901972988767312 1107 26-27 0.07489404775798647 1107 28-29 0.14779838599017836 1107 30-31 0.1886598759410134 1107 32-33 0.18864183410823188 1107 34-35 0.27418187991531084 1107 36-37 0.3367738764055659 1107 38-39 0.3724166333612615 1107 40-41 0.3193180276533738 1107 42-43 0.3258902237557493 1107 44-45 0.35936137475986385 1107 46-47 0.3560810100113301 1107 48-49 0.3487118702639833 1107 50-51 0.17683795937197289 1107 52-53 0.15706564299362924 1107 54-55 0.03767333118023686 1107 56-57 0.1887665734765065 1107 58-59 0.31272209412796315 1107 60-61 0.2709542854042084 1107 62-63 0.40507458808222196 1107 64-65 0.3280880982272123 1107 66-67 0.27502313866465755 1107 68-69 0.4160274150329357 1107 70-71 0.40380870355932075 1107 72-73 0.43461388488272945 1107 74-75 0.4059215177237938 1107 76-77 0.3018813583300357 1107 78-79 0.4404799502506478 1107 80-81 0.3315680029333805 1107 82-83 0.14109156760106956 1107 84-85 0.4330474793546024 1107 86-87 0.5448817807092468 1107 88-89 0.8087872978545079 1107 90-91 0.7898362559568959 1107 92-93 0.7556133697302485 1107 94-95 0.8550941414873812 1107 96-97 0.9476258229651044 1107 98-99 0.7421996313113581 1107 100-101 0.7826023972227851 1108 1 0.1008143702102231 1108 2 0.07643378658426059 1108 3 0.08937078920030217 1108 4 0.09087454161046082 1108 5 0.043961418434811605 1108 6 0.09547204443516222 1108 7 0.11928512231283861 1108 8 0.15530658365214123 1108 9 0.15660180426205272 1108 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-1.0874545363068222 2316 60-61 -1.0435641324701876 2316 62-63 -0.8942354964588404 2316 64-65 -0.8449025418694021 2316 66-67 -0.9625070545720646 2316 68-69 -0.8505139497013587 2316 70-71 -0.8776194365372945 2316 72-73 -0.6975645571462579 2316 74-75 -0.9128375402472138 2316 76-77 -0.810923593360787 2316 78-79 -0.8704137695561158 2316 80-81 -1.0616022869216906 2316 82-83 -1.0128033599351625 2316 84-85 -1.2246125447999852 2316 86-87 -1.3886840405467922 2316 88-89 -1.5086432577010456 2316 90-91 -1.7455253080527662 2316 92-93 -1.610266763119995 2316 94-95 -1.4271152486092369 2316 96-97 -1.5000560912861047 2316 98-99 -1.5565209302828436 2316 100-101 -1.0079312682361525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 17.0 5 72.0 6 164.0 7 406.0 8 918.0 9 1746.0 10 3022.0 11 4636.0 12 6938.0 13 9463.0 14 11265.0 15 15366.0 16 19884.0 17 28606.0 18 27813.0 19 18925.0 20 17052.0 21 17994.0 22 19870.0 23 22312.0 24 25371.0 25 30338.0 26 35580.0 27 42975.0 28 51855.0 29 62531.0 30 76589.0 31 93215.0 32 115112.0 33 145952.0 34 196174.0 35 279222.0 36 441789.0 37 680856.0 38 453155.0 39 23439.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.14533394145709 20.501944626155474 14.928921606605464 22.423799825781977 2 15.177950455490494 21.88565314817356 39.77121638256035 23.165180013775597 3 19.059944649341265 25.845986406243238 31.131340222026665 23.962728722388835 4 12.736656945018055 17.644070907085844 38.246171199205 31.373100948691103 5 12.80090504265369 38.390435857081364 34.870847903784195 13.937811196480748 6 30.154064589635826 37.62224332884995 17.588411589081918 14.635280492432312 7 26.639777523767606 34.216758654956855 21.788190629305923 17.355273191969626 8 25.111679297652543 35.41922135331656 20.541878184935886 18.92722116409501 9 25.895338339205125 17.385568770123662 21.656071042627524 35.06302184804368 10-11 24.2463094248772 27.095100268969713 28.00270949157444 20.655880814578648 12-13 25.611086638308038 24.928110907984983 28.241568150121132 21.21923430358585 14-15 22.453136729447387 26.057429471291556 27.396717956460293 24.092715842800764 16-17 20.94700404202905 29.31352607691682 28.02752505194079 21.711944829113346 18-19 20.846651999708115 28.170675756490464 30.302428435670738 20.680243808130683 20-21 21.91829722075814 27.124691298794584 29.95137274531953 21.00563873512774 22-23 21.54481950646054 27.1147437473852 29.847586066449644 21.492850679704613 24-25 21.61590589108382 27.22730065759555 30.066162153892556 21.09063129742808 26-27 21.57285408382281 27.085576635160493 30.436761324987234 20.904807956029465 28-29 21.04660318798054 27.6164660325508 30.376685086691317 20.96024569277734 30-31 21.34664012644294 27.262950520912838 30.041387181850297 21.349022170793923 32-33 21.25197902593174 27.473023347725128 29.98488911583546 21.290108510507665 34-35 21.766555110329374 27.189491648038526 29.869930724118515 21.174022517513585 36-37 21.836043095563646 26.888602119072715 30.07816779926948 21.197186986094163 38-39 21.478405616593164 27.29383702109041 30.150048048660675 21.077709313655753 40-41 21.49909963834551 27.39085694231344 30.022640732045865 21.08740268729519 42-43 21.347872592419613 27.575808208202567 29.482412576220895 21.593906623156926 44-45 21.490927439985693 27.464007856186427 29.29064836687881 21.75441633694907 46-47 21.78505208605056 27.658540832346812 28.897302939193548 21.659104142409078 48-49 21.8051902453034 27.645470181764438 28.86572152267371 21.683618050258453 50-51 21.41693312467383 27.94001973083729 28.923424657373392 21.71962248711549 52-53 21.74320018600076 28.205065836686366 28.405157562168842 21.646576415144033 54-55 21.34959251944134 27.714918274071316 28.721013288116986 22.21447591837036 56-57 21.658167912654793 28.04238227249044 28.389305224762662 21.910144590092102 58-59 21.965697249871795 27.63727784932362 28.513089533958947 21.88393536684564 60-61 21.677141665623807 27.665991520467937 28.919569252922827 21.73729756098543 62-63 21.544312979224948 27.685588845713305 28.915880525828076 21.854217649233675 64-65 21.3727463078854 27.89277291989657 28.754962492564438 21.979518279653586 66-67 21.381733072150194 27.811273764363804 28.37459459940054 22.432398564085464 68-69 21.172914601896693 27.87085505465755 28.603553428586192 22.35267691485957 70-71 21.48259521514149 27.797817341727754 28.362949260920047 22.356638182210716 72-73 21.509390790479742 27.494595946565713 28.49483649949524 22.501176763459306 74-75 21.13806027109007 28.2027005672956 28.075378619239604 22.583860542374723 76-77 21.241181158128146 27.68474107386621 28.384606782662836 22.689470985342815 78-79 21.272234469275737 27.82042018959345 28.28030399235305 22.627041348777762 80-81 21.267138580480832 27.97355860122142 28.089121457249462 22.67018136104829 82-83 21.221767322578796 27.76262626093182 28.351980938277205 22.66362547821218 84-85 21.1483497588593 27.63677081149088 28.077047599077375 23.13783183057245 86-87 21.102302102901184 27.63211889239548 28.2494527997378 23.016126204965538 88-89 21.214901779741975 27.469945898404923 28.039554314732783 23.275598007120315 90-91 20.950472846760466 27.339629873379863 28.36381683225341 23.346080447606262 92-93 21.208448004165557 27.66202682450339 28.001585569239822 23.12793960209123 94-95 20.938491777582477 27.426477507979513 28.013909273010835 23.621121441427174 96-97 20.65244194773392 27.344678127095822 28.179349822671895 23.82353010249836 98-99 20.792663840197704 27.45391666803662 27.660919677128987 24.09249981463669 100-101 20.827652890512933 26.87479775610556 28.520075427222142 23.77747392615936 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1430.0 1 1009.5 2 683.0 3 762.5 4 674.5 5 745.0 6 1259.5 7 2289.0 8 3521.0 9 4196.5 10 3976.0 11 3683.0 12 3807.0 13 4070.0 14 4480.5 15 4740.5 16 4853.5 17 5084.0 18 5526.5 19 6046.0 20 6762.0 21 7853.0 22 9268.5 23 11040.0 24 13072.0 25 15923.5 26 20838.5 27 26785.0 28 30957.0 29 35168.5 30 40213.5 31 45605.5 32 51579.5 33 58318.0 34 66053.0 35 72367.0 36 81054.0 37 88733.0 38 89002.5 39 88385.0 40 90043.5 41 92558.0 42 95425.0 43 99232.5 44 103860.5 45 107159.5 46 108407.0 47 116470.5 48 140178.5 49 139423.5 50 113949.0 51 99631.0 52 90840.0 53 87255.0 54 84963.5 55 79129.5 56 71965.0 57 63924.5 58 55988.5 59 49143.0 60 41485.0 61 32913.5 62 25455.0 63 19632.5 64 14644.5 65 10725.5 66 7637.0 67 5672.5 68 4088.0 69 2606.0 70 1752.5 71 1200.5 72 808.5 73 519.0 74 349.0 75 229.5 76 133.5 77 63.0 78 32.5 79 13.5 80 6.0 81 3.5 82 3.0 83 3.0 84 2.0 85 1.0 86 1.0 87 0.5 88 1.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.400552500089746 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.877733689454555E-4 16-17 0.0025330189929454582 18-19 3.522741645818187E-4 20-21 0.0 22-23 0.0 24-25 5.0324880654545525E-5 26-27 1.677496021818184E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012581220163636383 36-37 0.02340106950436367 38-39 0.010769524460072743 40-41 0.005502186951563644 42-43 0.0050660379858909164 44-45 0.0017445958626909116 46-47 0.0012245720959272745 48-49 0.003338217083418187 50-51 6.542234485090918E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.890728915636376E-4 60-61 0.001476196499200002 62-63 0.0013084468970181837 64-65 8.051980904727284E-4 66-67 0.003136917560800004 68-69 0.002516244032727276 70-71 5.0324880654545525E-5 72-73 0.0 74-75 0.0 76-77 0.0931345791313456 78-79 0.0032711172425454594 80-81 1.3419968174545473E-4 82-83 0.0 84-85 2.6839936349090947E-4 86-87 0.09216163143869104 88-89 0.4384303602624006 90-91 0.3890113274596369 92-93 0.0 94-95 0.001056822493745456 96-97 0.0 98-99 0.0 100-101 3.858240850181824E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2980633.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.94659239644279 #Duplication Level Percentage of deduplicated Percentage of total 1 67.08108381336237 27.467417964173652 2 15.514339468528664 12.705186690357761 3 6.483933565336944 7.964849544763977 4 3.343858841277146 5.476785000200671 5 1.9684886131809565 4.030145044047978 6 1.2344106390692084 3.03269455726796 7 0.8251620650342825 2.365130231657605 8 0.5896101705764096 1.9314021861911481 9 0.4442492980733591 1.6371445437554084 >10 2.2498040049835346 16.340038490687565 >50 0.15766224320467837 4.496638170420928 >100 0.09992820905794263 7.491772526320648 >500 0.004514272058280218 1.2771044002856626 >1k 0.002544407887339681 2.184050172516238 >5k 1.6415534757030202E-4 0.40843815201104616 >10k+ 2.46233021355453E-4 1.1912023253418513 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13468 0.4518503284369461 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11093 0.37216926740058237 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10883 0.36512378410894597 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6908 0.23176285037440036 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5245 0.17596933268872753 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4869 0.16335456260465478 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4211 0.14127871495752747 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3995 0.1340319321432729 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3629 0.12175266126356382 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3026 0.10152205924043652 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.677496021818184E-5 0.0 12-13 0.0 0.0 0.0 6.709984087272737E-5 0.0 14-15 0.0 0.0 0.0 6.709984087272737E-5 0.0 16-17 0.0 0.0 0.0 1.8452456240000025E-4 0.0 18-19 0.0 0.0 0.0 2.6839936349090947E-4 0.0 20-21 0.0 0.0 0.0 4.5292392589090977E-4 0.0 22-23 0.0 0.0 0.0 9.393977722181833E-4 0.0 24-25 0.0 0.0 0.0 0.0015265213798545479 0.0 26-27 3.354992043636368E-5 0.0 0.0 0.002231069709018185 0.0 28-29 6.709984087272737E-5 0.0 0.0 0.006810633848581828 0.0 30-31 6.709984087272737E-5 0.0 0.0 0.019442178892872756 0.0 32-33 6.709984087272737E-5 0.0 0.0 0.043564571686618245 0.0 34-35 6.709984087272737E-5 0.0 0.0 0.07964751111592738 0.0 36-37 6.709984087272737E-5 0.0 0.0 0.1403225422250911 0.0 38-39 6.709984087272737E-5 0.0 0.0 0.23021955403432762 0.0 40-41 6.709984087272737E-5 0.0 0.0 0.3282524215493823 0.0 42-43 6.709984087272737E-5 0.0 0.0 0.4302106297554915 0.0 44-45 8.387480109090922E-5 0.0 0.0 0.5535233623193463 0.0 46-47 1.0064976130909105E-4 0.0 0.0 0.703306982107492 0.0 48-49 1.0064976130909105E-4 0.0 0.0 0.8631555780265467 0.0 50-51 1.0064976130909105E-4 0.0 0.0 1.036524791881456 0.0 52-53 1.0064976130909105E-4 0.0 0.0 1.2070254875390563 0.0 54-55 1.0064976130909105E-4 0.0 0.0 1.4007595030988385 0.0 56-57 1.0064976130909105E-4 0.0 0.0 1.6307106577696753 0.0 58-59 1.0064976130909105E-4 0.0 0.0 1.8860926521312753 0.0 60-61 1.8452456240000025E-4 0.0 0.0 2.172474772976076 0.0 62-63 2.348494430545458E-4 0.0 0.0 2.4665901504814585 0.0 64-65 2.348494430545458E-4 0.0 0.0 2.7675329367956403 0.0 66-67 2.5162440327272765E-4 0.0 0.0 3.11823025511695 0.0 68-69 2.6839936349090947E-4 0.0 0.0 3.518430481042114 0.0 70-71 2.6839936349090947E-4 0.0 0.0 3.952348377005824 0.0 72-73 3.3549920436363686E-4 0.0 0.0 4.398126169843788 0.0 74-75 3.3549920436363686E-4 0.0 0.0 4.86505718751688 0.0 76-77 3.3549920436363686E-4 0.0 0.0 5.357301620159207 0.0 78-79 3.3549920436363686E-4 0.0 0.0 5.924932724022044 0.0 80-81 3.3549920436363686E-4 0.0 0.0 6.53862786864401 0.0 82-83 3.3549920436363686E-4 0.0 0.0 7.158462648705829 0.0 84-85 3.3549920436363686E-4 0.0 0.0 7.800339726494339 0.0 86-87 3.3549920436363686E-4 0.0 0.0 8.48668722382125 0.0 88-89 3.3549920436363686E-4 0.0 0.0 9.213009451348086 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGATCG 70 2.0662734E-4 33.8945 5 GTACATG 11305 0.0 23.387321 1 TACATGG 11260 0.0 22.630447 2 GAGTACT 11740 0.0 22.25271 12-13 ACATGGG 11435 0.0 21.620155 3 ACCGGAC 110 0.002895259 21.569227 3 CATGGGG 4800 0.0 20.463804 4 AGTACTT 12390 0.0 20.185196 12-13 GTACTTT 12825 0.0 20.148106 14-15 CTCCGAC 570 0.0 18.7318 94-95 GTGTACG 285 4.1385647E-8 18.386791 1 TACCCGA 160 0.0011996763 17.794611 2 GTACGCT 160 0.0011996763 17.794611 3 AGTACAT 8770 0.0 17.584948 2 TTGCGTT 730 0.0 17.55085 4 CTACACT 1170 0.0 17.034159 4 GTATCAA 31205 0.0 16.915066 1 ACTTTTT 15515 0.0 16.654118 16-17 CTTTGCG 685 0.0 16.625624 2 CATGGGA 6840 0.0 16.580555 4 >>END_MODULE