FastQCFastQC Report
Fri 27 May 2016
SRR939008_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939008_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2996279
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138920.46364173696775235No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT105250.3512690240127838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104540.3488994182450967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67520.22534617103413934No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT56970.19013583181005508No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA48590.16216780880552178No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44910.14988590848849523No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA38670.12906007751614584No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36930.12325287464885613No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG120250.023.4196591
TACATGG119150.023.2801482
GAGTACT111050.021.9516512-13
ACATGGG119450.021.9139753
AGTACTT116900.019.57764612-13
GTACTTT124050.019.57586314-15
CATGGGG52250.019.5681424
TTTGCGT6550.018.0667863
CCCTATA5050.017.8091982
CTTTGCG6850.017.275542
CATGGGA68150.017.0864334
TTGCGTT7000.016.905354
AGTACAT90750.016.8997612
GTATCAA316950.016.6637861
ACTTTTT149300.016.64686616-17
GTATAGA7750.016.5173841
GTGTAGC16500.015.803671
AGAGTAC232900.015.36455310-11
CAACGCA348550.015.08772855
GGTATCA262900.015.0763041
Produced by FastQC (version 0.11.5)