##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939008_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2996279 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81588496932362 33.0 31.0 34.0 30.0 34.0 2 31.879111391162173 33.0 31.0 34.0 30.0 34.0 3 31.923782464850568 34.0 31.0 34.0 30.0 34.0 4 35.467117047511266 37.0 35.0 37.0 33.0 37.0 5 35.50737998697718 37.0 35.0 37.0 33.0 37.0 6 35.59280127117668 37.0 35.0 37.0 33.0 37.0 7 35.50784356196469 37.0 35.0 37.0 33.0 37.0 8 35.49624183862718 37.0 35.0 37.0 33.0 37.0 9 37.09828490604513 39.0 37.0 39.0 33.0 39.0 10-11 36.9900501588804 39.0 37.0 39.0 33.0 39.0 12-13 36.87548322435928 39.0 37.0 39.0 33.0 39.0 14-15 38.0727772680715 40.0 38.0 41.0 33.0 41.0 16-17 38.00779133051361 40.0 38.0 41.0 33.0 41.0 18-19 37.976891504429325 40.0 38.0 41.0 33.0 41.0 20-21 37.86629332582179 40.0 38.0 41.0 32.5 41.0 22-23 37.93561564193455 40.0 38.0 41.0 33.0 41.0 24-25 37.87124696999178 40.0 38.0 41.0 32.0 41.0 26-27 37.78433900848353 40.0 38.0 41.0 32.0 41.0 28-29 37.65975281340623 40.0 37.0 41.0 32.0 41.0 30-31 37.47643009879921 40.0 37.0 41.0 32.0 41.0 32-33 37.281257519743654 40.0 36.5 41.0 31.5 41.0 34-35 36.960511521123365 40.0 36.0 41.0 30.5 41.0 36-37 36.688480612119236 40.0 36.0 41.0 30.0 41.0 38-39 36.480798850841325 39.0 35.5 41.0 30.0 41.0 40-41 36.342047252608985 39.0 35.0 41.0 30.0 41.0 42-43 36.1283069767535 39.0 35.0 41.0 29.5 41.0 44-45 36.03350238746125 39.0 35.0 41.0 29.0 41.0 46-47 35.788426578432784 39.0 35.0 41.0 27.0 41.0 48-49 35.58764754550561 39.0 35.0 41.0 26.5 41.0 50-51 34.42259616010392 38.0 34.0 40.0 24.5 40.5 52-53 34.57904520907432 38.5 34.5 39.5 24.5 40.5 54-55 35.08805455032726 39.0 35.0 40.5 26.0 41.0 56-57 34.99974668580596 39.0 35.0 41.0 25.0 41.0 58-59 34.81269150836755 38.5 34.5 40.5 25.0 41.0 60-61 34.54703817635141 38.0 34.0 40.0 24.0 41.0 62-63 34.20527143834069 38.0 34.0 40.0 23.0 41.0 64-65 33.80048253183365 37.0 34.0 40.0 22.0 41.0 66-67 33.417016072268304 37.0 33.0 39.5 21.5 41.0 68-69 33.02363865314278 36.0 33.0 39.0 20.5 41.0 70-71 32.599950471901984 36.0 32.5 39.0 19.5 40.5 72-73 32.156851715077266 35.0 32.0 38.0 18.0 40.0 74-75 31.685498413198502 35.0 31.5 37.0 14.5 39.5 76-77 31.27094005598277 35.0 31.0 37.0 11.5 39.0 78-79 30.828739913739675 35.0 31.0 36.0 7.5 39.0 80-81 30.375792608098244 34.5 30.5 36.0 4.5 37.5 82-83 29.98329594807426 34.0 30.0 35.5 2.0 37.0 84-85 29.61864115457873 34.0 30.0 35.0 2.0 37.0 86-87 29.225412086124155 34.0 29.5 35.0 2.0 36.0 88-89 28.794503782858673 34.0 29.0 35.0 2.0 36.0 90-91 28.360276195908327 34.0 29.0 35.0 2.0 35.5 92-93 27.93456634045094 34.0 28.5 35.0 2.0 35.0 94-95 27.455491127495137 34.0 27.0 35.0 2.0 35.0 96-97 26.862263327280267 33.5 25.5 35.0 2.0 35.0 98-99 26.15057676538133 33.0 24.5 35.0 2.0 35.0 100-101 23.524763381514205 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44376689400350955 1101 2 0.45309170309459645 1101 3 0.3431690995562704 1101 4 0.2576908096281585 1101 5 0.17317003306157375 1101 6 0.34822448369651937 1101 7 0.30936834712464645 1101 8 0.3942382161660589 1101 9 0.30459211195148583 1101 10-11 0.5031844971078456 1101 12-13 0.6001659528386014 1101 14-15 0.34968602435829865 1101 16-17 0.5720956141969751 1101 18-19 0.5862744909548994 1101 20-21 0.7661461254537798 1101 22-23 0.6010593120148116 1101 24-25 0.6828047029777835 1101 26-27 0.6712174285982826 1101 28-29 0.572716670727516 1101 30-31 0.6918707624039584 1101 32-33 0.6207326540155549 1101 34-35 0.592904657963075 1101 36-37 0.5898878202671014 1101 38-39 0.6433420265165424 1101 40-41 0.6203080207707075 1101 42-43 0.6211215509876169 1101 44-45 0.643134360938511 1101 46-47 0.7154106678264966 1101 48-49 0.6930850344438895 1101 50-51 0.5976184002708536 1101 52-53 0.6521364550679181 1101 54-55 0.46269911106188744 1101 56-57 0.5638139933757813 1101 58-59 0.5369204880520684 1101 60-61 0.4821136763991092 1101 62-63 0.5568146785571386 1101 64-65 0.587874392897838 1101 66-67 0.5317911554993273 1101 68-69 0.531404177187639 1101 70-71 0.3581216884682732 1101 72-73 0.3365919756738833 1101 74-75 0.3388951637935129 1101 76-77 0.3777636914371385 1101 78-79 0.19345697363105074 1101 80-81 0.030254251453420977 1101 82-83 0.11887521101379761 1101 84-85 0.037121600615968475 1101 86-87 0.06553288543376468 1101 88-89 -0.1296813227492457 1101 90-91 -0.027971268727817744 1101 92-93 0.13460043421646972 1101 94-95 0.1343474604985282 1101 96-97 0.08349872043932649 1101 98-99 0.08981597397553642 1101 100-101 0.3121465476420262 1102 1 0.28176352250353887 1102 2 0.2116712948721151 1102 3 0.1961439547571544 1102 4 0.2593612746511127 1102 5 0.265535634849968 1102 6 0.3717128074102547 1102 7 0.3532258259918919 1102 8 0.2550409150240682 1102 9 0.4192236290772513 1102 10-11 0.3319399410803001 1102 12-13 0.43658343748823114 1102 14-15 0.575789734640388 1102 16-17 0.4676699829215565 1102 18-19 0.5782342769965112 1102 20-21 0.6173766167254158 1102 22-23 0.585654336940955 1102 24-25 0.6025133793700022 1102 26-27 0.6302713464320249 1102 28-29 0.5943079104234457 1102 30-31 0.6255199072362316 1102 32-33 0.6094506679652909 1102 34-35 0.5765285048875484 1102 36-37 0.515035093468704 1102 38-39 0.5427138663829894 1102 40-41 0.37272634790672754 1102 42-43 0.3242506677266448 1102 44-45 0.4894276151147423 1102 46-47 0.44530090366310304 1102 48-49 0.5189044703355208 1102 50-51 0.35483523404015216 1102 52-53 0.3426126671330394 1102 54-55 0.4304947319461192 1102 56-57 0.4038917083412841 1102 58-59 0.4077889821957186 1102 60-61 0.3886543578441817 1102 62-63 0.35045505914752084 1102 64-65 0.44222761490310347 1102 66-67 0.38243719126665354 1102 68-69 0.29765248062159344 1102 70-71 0.41190500159004984 1102 72-73 0.33530337563911417 1102 74-75 0.23298669688299256 1102 76-77 0.22630700863145137 1102 78-79 0.2417471563070741 1102 80-81 0.09481592407388106 1102 82-83 -0.009070544468777086 1102 84-85 0.09822103499464419 1102 86-87 0.24506610082245572 1102 88-89 0.2865401801874121 1102 90-91 0.2758363967066373 1102 92-93 0.17255765860204875 1102 94-95 0.24403876125658286 1102 96-97 0.13986843051468156 1102 98-99 0.2254563478301641 1102 100-101 0.3532430182924422 1103 1 0.12801554288394001 1103 2 0.2674189818254611 1103 3 0.38041605469885553 1103 4 0.32981595185887613 1103 5 0.24237963019897535 1103 6 0.2020733819452829 1103 7 0.23424626589532238 1103 8 0.19686723930696104 1103 9 0.2950945729616876 1103 10-11 0.35161344709694475 1103 12-13 0.3809762099085461 1103 14-15 0.41092158640825005 1103 16-17 0.42494294375152464 1103 18-19 0.41371427647553816 1103 20-21 0.5096804773406873 1103 22-23 0.5616491276858611 1103 24-25 0.5196915735374645 1103 26-27 0.496118854031522 1103 28-29 0.48499291291213353 1103 30-31 0.5284262159817672 1103 32-33 0.5556368512065433 1103 34-35 0.4271876296519892 1103 36-37 0.5482872540531005 1103 38-39 0.5684220178533579 1103 40-41 0.635533880513087 1103 42-43 0.5909830183040512 1103 44-45 0.6931383985383874 1103 46-47 0.6642774502676474 1103 48-49 0.48157828561816984 1103 50-51 0.4515077387091537 1103 52-53 0.5178229028928243 1103 54-55 0.701194165997002 1103 56-57 0.6484994091413583 1103 58-59 0.5407645527353182 1103 60-61 0.5820162861521823 1103 62-63 0.5039458065778106 1103 64-65 0.39592901308678563 1103 66-67 0.412521482213684 1103 68-69 0.4221479441668947 1103 70-71 0.3535233821547621 1103 72-73 0.43813112928766884 1103 74-75 0.33397545425934894 1103 76-77 0.2568498355883122 1103 78-79 0.19770120361839005 1103 80-81 0.2290507128983208 1103 82-83 0.3462966508103591 1103 84-85 0.258606741542323 1103 86-87 0.3567621400979597 1103 88-89 0.3197133004515358 1103 90-91 0.10244083220935352 1103 92-93 -0.07801777791376452 1103 94-95 0.09323512933471179 1103 96-97 0.14277396034914602 1103 98-99 0.0330927025978518 1103 100-101 0.18721569715659925 1104 1 0.035209649927121944 1104 2 0.08659853896122272 1104 3 0.09105976918820602 1104 4 0.19434754849707048 1104 5 0.10062709978300433 1104 6 0.1458510428863633 1104 7 0.08340515390585779 1104 8 0.18457445719683818 1104 9 0.19431794136347946 1104 10-11 0.20999952521581378 1104 12-13 0.26613199617917616 1104 14-15 0.40464443964000907 1104 16-17 0.20150056339424083 1104 18-19 0.3609719215713767 1104 20-21 0.31837308973015155 1104 22-23 0.33534532869017397 1104 24-25 0.23453548811172453 1104 26-27 0.19752459636373487 1104 28-29 0.2808272777073597 1104 30-31 0.24493455480584458 1104 32-33 0.3299635839316437 1104 34-35 0.2279831703461781 1104 36-37 0.2398926126191654 1104 38-39 0.28199994539151163 1104 40-41 0.3133869539703724 1104 42-43 0.406736376881085 1104 44-45 0.34649010747443043 1104 46-47 0.5356012390571578 1104 48-49 0.5460477701764006 1104 50-51 0.38135598978605145 1104 52-53 0.3140224238956364 1104 54-55 0.28119067336107406 1104 56-57 0.2681562183978272 1104 58-59 0.3001858197220244 1104 60-61 0.320017299290825 1104 62-63 0.28063655024501344 1104 64-65 0.21857820859636945 1104 66-67 0.23797401041093025 1104 68-69 0.28960845631546306 1104 70-71 0.34239512363887314 1104 72-73 0.20300231683624048 1104 74-75 0.24626348728208214 1104 76-77 0.18684940929227878 1104 78-79 0.192114419593171 1104 80-81 0.1179583405309792 1104 82-83 0.05428935242283117 1104 84-85 0.13352456737272433 1104 86-87 0.15587163012076743 1104 88-89 0.2776120174999086 1104 90-91 0.3127990405394776 1104 92-93 0.3796285270095545 1104 94-95 0.31286409223623934 1104 96-97 0.28838820930652886 1104 98-99 0.21833054462251766 1104 100-101 0.6315360753285439 1105 1 -0.0018662024760374152 1105 2 0.023734319354133504 1105 3 0.061216113580833564 1105 4 -0.010783785625640974 1105 5 0.008081368444045722 1105 6 0.06745580812813046 1105 7 0.015508916320378319 1105 8 0.07571695532408995 1105 9 0.11939393875370996 1105 10-11 0.08161516448797812 1105 12-13 0.15686538911830183 1105 14-15 0.13284273362546628 1105 16-17 0.12033825036716195 1105 18-19 0.1261571425558401 1105 20-21 0.21388477327169397 1105 22-23 0.08271550484946744 1105 24-25 0.06209849707676085 1105 26-27 0.04633751471185121 1105 28-29 0.05637786611322326 1105 30-31 0.048822503963712904 1105 32-33 0.12983200816381668 1105 34-35 0.15308876926361137 1105 36-37 0.20390802957782483 1105 38-39 0.23523496943519717 1105 40-41 0.1250466934769392 1105 42-43 0.06891202871879187 1105 44-45 -0.0025732760405432487 1105 46-47 0.2508169073267368 1105 48-49 0.19660343312978767 1105 50-51 0.09831234867737493 1105 52-53 0.12583778285946323 1105 54-55 0.2536287517990772 1105 56-57 0.23637468975079656 1105 58-59 0.26628627159839624 1105 60-61 0.36889026645125256 1105 62-63 0.2787822864168703 1105 64-65 0.2907231715038421 1105 66-67 0.22080452052377808 1105 68-69 0.3241982637242984 1105 70-71 0.2281653674588071 1105 72-73 0.40156634071077946 1105 74-75 0.4090246452454984 1105 76-77 0.22002523028157484 1105 78-79 0.2219788566808667 1105 80-81 0.1311066139765984 1105 82-83 0.10048585823439637 1105 84-85 0.1254381550778163 1105 86-87 0.26054806730511615 1105 88-89 0.25773271923733887 1105 90-91 0.36665291773542563 1105 92-93 0.5075107491780777 1105 94-95 0.4647473534787778 1105 96-97 0.2713937913087996 1105 98-99 0.2586742848056822 1105 100-101 0.6521999952956854 1106 1 0.1560425320607095 1106 2 0.12350826832247108 1106 3 0.20690377349492195 1106 4 0.13198633714143426 1106 5 0.09802151069423104 1106 6 0.1711930443432479 1106 7 0.11377538893839301 1106 8 0.16936381807379064 1106 9 0.2096678759852324 1106 10-11 0.18628899843553626 1106 12-13 0.31776912377241473 1106 14-15 0.2656938404069962 1106 16-17 0.32635524527338333 1106 18-19 0.2844862897589593 1106 20-21 0.30474836802299166 1106 22-23 0.2243438703807925 1106 24-25 0.332623132648699 1106 26-27 0.262659314669591 1106 28-29 0.28905620699724466 1106 30-31 0.1725258207872642 1106 32-33 0.24252572427377572 1106 34-35 0.21626064316328097 1106 36-37 -5.16749183240961E-4 1106 38-39 -0.016709233752521868 1106 40-41 0.09838053540553204 1106 42-43 0.11550001961102652 1106 44-45 0.17376953281370788 1106 46-47 0.15093803124401717 1106 48-49 0.08956568753696104 1106 50-51 -0.0322382252528115 1106 52-53 0.06202625261369121 1106 54-55 -0.010978534414853414 1106 56-57 0.011141831367183386 1106 58-59 0.059146885199020005 1106 60-61 0.20562015542099488 1106 62-63 0.1397783897947278 1106 64-65 -0.01573148918487277 1106 66-67 0.15921969857124196 1106 68-69 0.14175630226799996 1106 70-71 0.10070920445793519 1106 72-73 0.006241700444121534 1106 74-75 0.029054339384131822 1106 76-77 -0.02997488708655993 1106 78-79 0.15696019514709647 1106 80-81 0.08885631468784894 1106 82-83 0.12592540190708235 1106 84-85 0.13755829310274947 1106 86-87 0.0887909489276133 1106 88-89 0.23660070355695595 1106 90-91 0.1627176814733673 1106 92-93 0.18514625069213153 1106 94-95 0.2375966997382548 1106 96-97 0.23837015336602718 1106 98-99 0.21738147477774916 1106 100-101 0.6576905941079154 1107 1 0.09545744534472078 1107 2 0.06505119898538325 1107 3 0.09052120722689949 1107 4 0.12328179579684928 1107 5 0.11263589321212208 1107 6 0.08710047817889688 1107 7 0.017600636957524785 1107 8 0.12373901028645662 1107 9 0.1829647421844811 1107 10-11 0.1438935938012449 1107 12-13 0.15720321512915802 1107 14-15 0.16210990207169118 1107 16-17 0.12076826378685013 1107 18-19 0.12322639980538952 1107 20-21 0.18494317807944327 1107 22-23 0.10938508883919695 1107 24-25 0.1360193792752611 1107 26-27 0.20778946640098184 1107 28-29 0.1981580166470991 1107 30-31 0.2251580544625753 1107 32-33 0.1606555684648896 1107 34-35 0.12389599255551786 1107 36-37 0.012452868833321418 1107 38-39 0.10003892532527203 1107 40-41 0.016172488447473654 1107 42-43 -0.022265694602282338 1107 44-45 0.06082715572326691 1107 46-47 0.16056018289707197 1107 48-49 -0.029794548082648475 1107 50-51 0.13548692128954798 1107 52-53 0.06697408019302031 1107 54-55 0.10259222691431802 1107 56-57 0.11861373520267904 1107 58-59 0.1694775312984973 1107 60-61 0.0016062942241674705 1107 62-63 0.017279573306417717 1107 64-65 0.264331608402145 1107 66-67 0.18162924775406708 1107 68-69 0.09612544734061146 1107 70-71 0.06828789502750254 1107 72-73 0.05969254410653946 1107 74-75 0.1832217979381845 1107 76-77 0.07665076269303484 1107 78-79 0.19825576898613306 1107 80-81 0.0314919923533985 1107 82-83 0.12496511606740768 1107 84-85 0.10139766263368699 1107 86-87 0.1883311935085139 1107 88-89 0.3110435891830754 1107 90-91 0.3163288031429765 1107 92-93 0.12969990840557344 1107 94-95 0.09583405632025332 1107 96-97 0.16440038445892569 1107 98-99 0.18698486301911643 1107 100-101 0.40079938849248364 1108 1 0.1064904860313014 1108 2 0.16836007859656732 1108 3 0.12099706915020647 1108 4 0.1247248470205875 1108 5 0.17407974235001689 1108 6 0.11774707026405196 1108 7 0.1514192898437443 1108 8 0.18134371891871126 1108 9 0.26610540271659744 1108 10-11 0.21090829184940674 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2316 92-93 -2.6936988704510085 2316 94-95 -2.862507259032089 2316 96-97 -3.1132121268460082 2316 98-99 -3.5420443687519914 2316 100-101 -4.208928266425492 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8926.0 3 2115.0 4 2101.0 5 2438.0 6 2888.0 7 3571.0 8 4505.0 9 5630.0 10 7097.0 11 9377.0 12 12857.0 13 18741.0 14 18276.0 15 17500.0 16 22052.0 17 29019.0 18 25271.0 19 17638.0 20 17254.0 21 18495.0 22 20299.0 23 22759.0 24 25858.0 25 30094.0 26 34660.0 27 40173.0 28 47750.0 29 57195.0 30 67982.0 31 82717.0 32 102426.0 33 130829.0 34 174279.0 35 249036.0 36 380212.0 37 603842.0 38 581784.0 39 98614.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25958354042516 19.819533068590488 14.362081862772168 22.558801528212182 2 14.928949292632845 21.380749685924506 40.39293090472782 23.29737011671483 3 19.138750546586177 25.72180275061874 30.893602010434996 24.245844692360087 4 12.465938962085504 17.01976108161419 38.69744844572084 31.81685151057947 5 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30.000142021602322 21.194986470353825 26-27 21.61817054114351 26.978743511905478 30.386856186530963 21.01622976042005 28-29 21.093917859339907 27.48727130196111 30.3538472796825 21.064963559016483 30-31 21.385901053033937 27.21462566441109 29.974629754122788 21.424843528432184 32-33 21.251433747261554 27.410056895501583 29.983802372912276 21.354706984324586 34-35 21.784851732498378 27.196943022383596 29.724892785676214 21.29331245944181 36-37 21.894214957599004 26.81398263542294 30.035981638068392 21.25582076890966 38-39 21.505632406357595 27.272127694281266 30.035886829614082 21.186353069747057 40-41 21.548590920429998 27.366610651419244 29.941617651228164 21.14318077692259 42-43 21.37928951386908 27.52665581528032 29.343496719413352 21.750557951437248 44-45 21.4896351381313 27.483512678533273 29.232654975283072 21.79419720805235 46-47 21.821992867631636 27.629133627019087 28.867900146842878 21.680973358506396 48-49 21.775273513205747 27.605572287083703 28.898081437761793 21.721072761948758 50-51 21.41435856137442 27.893272792793276 28.973556202280644 21.71881244355166 52-53 21.674332291667717 28.15317016478996 28.48609696990449 21.686400573637837 54-55 21.336742114452406 27.639171802467704 28.82477107286815 22.19931501021174 56-57 21.71124604352857 27.950239066178778 28.440804610044268 21.89771028024838 58-59 21.999025193136756 27.577118380310576 28.551522569463376 21.872333857089288 60-61 21.72159634671526 27.52924517686626 29.014834258465672 21.734324217952803 62-63 21.59184313220072 27.57945438075206 29.008715287951542 21.81998719909568 64-65 21.477411829957703 27.745000345810954 28.91013145516578 21.867456369065565 66-67 21.464194219895408 27.644247840800745 28.483449091115748 22.4081088481881 68-69 21.20667687125712 27.72080858367571 28.822393955282806 22.250120589784363 70-71 21.534885267061025 27.61615578926303 28.58599404937987 22.262964894296076 72-73 21.547345952429517 27.2814384124482 28.821146093275885 22.350069541846395 74-75 21.19749928403727 27.98287867042112 28.458351784971462 22.361270260570144 76-77 21.363261157307214 27.478115736092413 28.638283002127917 22.520340104472456 78-79 21.500103620335093 27.536680340076064 28.623292676685256 22.339923362903587 80-81 21.479549690303436 27.656264918299943 28.446610755220657 22.417574636175964 82-83 21.425943845382648 27.46619180292819 28.748370886435808 22.35949346525335 84-85 21.444463130221177 27.370343926826052 28.481621755346342 22.703571187606432 86-87 21.422224236062338 27.279041882000715 28.720280125160162 22.57845375677678 88-89 21.53211483132165 27.131016212416665 28.543245699162778 22.79362325709891 90-91 21.326524154673304 26.980841673167145 28.862883614310153 22.829750557849398 92-93 21.676900129516607 27.212723684751705 28.57745773083209 22.532918454899598 94-95 21.510093805052424 26.93721585100243 28.603792424886958 22.94889791905818 96-97 21.275095984055007 26.765227810942427 28.78900529949829 23.170670905504284 98-99 21.476254645381363 26.860087333253535 28.335109757495857 23.328548263869244 100-101 21.632810283970656 26.29861312753755 28.951999073516156 23.11657751497564 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4075.0 1 2421.5 2 876.0 3 976.0 4 874.0 5 957.0 6 1496.0 7 2688.0 8 4281.5 9 5048.0 10 4751.5 11 4504.0 12 4785.0 13 5111.5 14 5250.5 15 5581.0 16 5908.0 17 6110.0 18 6388.0 19 6732.5 20 7313.5 21 8126.0 22 9361.5 23 11102.5 24 12974.0 25 15631.5 26 20774.0 27 26657.0 28 30565.0 29 34814.0 30 39826.5 31 45219.5 32 50952.0 33 57889.0 34 66394.5 35 73012.5 36 81485.0 37 89138.0 38 89209.0 39 88270.0 40 90127.5 41 93331.5 42 96087.0 43 99217.0 44 103353.0 45 106319.0 46 108476.0 47 116361.0 48 140714.5 49 139712.5 50 111790.5 51 98025.0 52 90867.0 53 87589.5 54 84739.0 55 78351.0 56 71053.5 57 63638.0 58 56008.0 59 49006.0 60 41834.0 61 33350.5 62 25847.0 63 20200.5 64 15292.0 65 11368.5 66 8219.0 67 6135.5 68 4411.0 69 2941.5 70 2020.5 71 1384.0 72 940.5 73 626.0 74 451.5 75 325.5 76 224.5 77 135.0 78 75.5 79 50.0 80 38.5 81 25.5 82 17.0 83 18.0 84 18.0 85 14.0 86 11.5 87 10.0 88 9.5 89 8.0 90 4.0 91 1.0 92 2.5 93 3.0 94 2.0 95 2.5 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28281745458283425 2 0.08583980330269644 3 0.09004501917211315 4 0.10422927904911392 5 0.10596476496347637 6 0.08357032172237631 7 0.09271499750190153 8 0.0887434047363413 9 0.09368286464644981 10-11 0.08722485456127417 12-13 0.09178050508647559 14-15 0.09987387689864662 16-17 0.08947764877703311 18-19 0.09955681697198424 20-21 0.10157598808388671 22-23 0.11425838515038152 24-25 0.12603966453057275 26-27 0.12932707534912471 28-29 0.12121701617239249 30-31 0.11357420320337326 32-33 0.10840112018940826 34-35 0.37947067012117364 36-37 0.5806702246352893 38-39 0.5644667936463861 40-41 0.5286723966626606 42-43 0.5549883705756373 44-45 0.5756306405378138 46-47 0.5641664210842848 48-49 0.5853259993478578 50-51 0.578667740887948 52-53 0.5805867878124834 54-55 0.5710583026480511 56-57 0.5457101958796227 58-59 0.5407206738758307 60-61 0.4883890986119784 62-63 0.5607621987138047 64-65 0.5931690606916112 66-67 0.6057346462061778 68-69 0.6076703804952742 70-71 0.6041493465728659 72-73 0.5959558505733278 74-75 0.5931356859624888 76-77 0.5777833105662056 78-79 0.5557393019808903 80-81 0.5509333409872712 82-83 0.5639661727095507 84-85 0.5735780946967889 86-87 0.5639161106158672 88-89 0.5762480730265773 90-91 0.5891474058323674 92-93 0.597290839738222 94-95 0.5963396599582348 96-97 0.59371974372213 98-99 0.5979917090497914 100-101 0.5765150708595562 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2996279.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.38554073825734 #Duplication Level Percentage of deduplicated Percentage of total 1 68.50746498615858 29.037259480455624 2 14.974773078125583 12.694277086981021 3 6.2527542496336315 7.950791099224739 4 3.0921251748077196 5.242455902584147 5 1.8304671917327222 3.8792670862615406 6 1.1996392353193748 3.0508414607904744 7 0.8148590671867875 2.417676953172877 8 0.5375205893180985 1.8226480668955485 9 0.4166554575757676 1.5894150183805453 >10 2.1232931790346847 15.851965949551314 >50 0.15196971546837 4.501515926623333 >100 0.09184190089506096 7.127623750102561 >500 0.0038711019338017356 1.1397445653154177 >1k 0.002370062408382402 2.1099857375238473 >5k 1.580041605588268E-4 0.4168604781063031 >10k+ 2.370062408382402E-4 1.1676714380307573 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13892 0.46364173696775235 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10525 0.3512690240127838 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10454 0.3488994182450967 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6752 0.22534617103413934 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5697 0.19013583181005508 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4859 0.16216780880552178 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4491 0.14988590848849523 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3867 0.12906007751614584 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3693 0.12325287464885613 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.337472912235476E-5 0.0 12-13 0.0 0.0 0.0 3.337472912235476E-5 0.0 14-15 0.0 0.0 0.0 3.337472912235476E-5 0.0 16-17 0.0 0.0 0.0 1.0012418736706428E-4 0.0 18-19 0.0 0.0 0.0 2.0024837473412859E-4 0.0 20-21 0.0 0.0 0.0 3.003725621011929E-4 0.0 22-23 0.0 0.0 0.0 7.175566761306273E-4 0.0 24-25 0.0 0.0 0.0 0.0015185501750671418 0.0 26-27 0.0 0.0 0.0 0.002536479413298962 0.0 28-29 0.0 0.0 0.0 0.006908568928327436 0.0 30-31 0.0 0.0 0.0 0.018940158776936326 0.0 32-33 0.0 0.0 0.0 0.04500582222149539 0.0 34-35 0.0 0.0 0.0 0.0853558697304223 0.0 36-37 0.0 0.0 0.0 0.14598106518117973 0.0 38-39 0.0 0.0 0.0 0.23772819553853297 0.0 40-41 0.0 0.0 0.0 0.33813606810313723 0.0 42-43 0.0 0.0 0.0 0.44508538757572313 0.0 44-45 1.668736456117738E-5 0.0 0.0 0.5751633943301008 0.0 46-47 3.337472912235476E-5 0.0 0.0 0.7305227583946622 0.0 48-49 3.337472912235476E-5 0.0 0.0 0.8934581859699982 0.0 50-51 5.006209368353214E-5 0.0 0.0 1.0628182489013873 0.0 52-53 6.674945824470952E-5 0.0 0.0 1.236450277160438 0.0 54-55 6.674945824470952E-5 0.0 0.0 1.434846354428276 0.0 56-57 6.674945824470952E-5 0.0 0.0 1.6705720662194676 0.0 58-59 6.674945824470952E-5 0.0 0.0 1.9291260927303497 0.0 60-61 6.674945824470952E-5 0.0 0.0 2.21656594729663 0.0 62-63 6.674945824470952E-5 0.0 0.0 2.5083111419196946 0.0 64-65 6.674945824470952E-5 0.0 0.0 2.810836374049279 0.0 66-67 6.674945824470952E-5 0.0 0.0 3.1617883381354006 0.0 68-69 6.674945824470952E-5 0.0 0.0 3.5513214890869644 0.0 70-71 6.674945824470952E-5 0.0 0.0 3.9855100276042386 0.0 72-73 1.0012418736706428E-4 0.0 0.0 4.429861171139271 0.0 74-75 1.0012418736706428E-4 0.0 0.0 4.900478226493594 0.0 76-77 1.0012418736706428E-4 0.0 0.0 5.400131296184368 0.0 78-79 1.0012418736706428E-4 1.668736456117738E-5 0.0 5.966550511484412 0.0 80-81 1.0012418736706428E-4 3.337472912235476E-5 0.0 6.583048507832548 0.0 82-83 1.0012418736706428E-4 3.337472912235476E-5 0.0 7.204686212465528 0.0 84-85 1.0012418736706428E-4 3.337472912235476E-5 0.0 7.852706640469728 0.0 86-87 1.0012418736706428E-4 3.337472912235476E-5 0.0 8.537556082060448 0.0 88-89 1.0012418736706428E-4 3.337472912235476E-5 0.0 9.258266670093139 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12025 0.0 23.419659 1 TACATGG 11915 0.0 23.280148 2 GAGTACT 11105 0.0 21.95165 12-13 ACATGGG 11945 0.0 21.913975 3 AGTACTT 11690 0.0 19.577646 12-13 GTACTTT 12405 0.0 19.575863 14-15 CATGGGG 5225 0.0 19.568142 4 TTTGCGT 655 0.0 18.066786 3 CCCTATA 505 0.0 17.809198 2 CTTTGCG 685 0.0 17.27554 2 CATGGGA 6815 0.0 17.086433 4 TTGCGTT 700 0.0 16.90535 4 AGTACAT 9075 0.0 16.899761 2 GTATCAA 31695 0.0 16.663786 1 ACTTTTT 14930 0.0 16.646866 16-17 GTATAGA 775 0.0 16.517384 1 GTGTAGC 1650 0.0 15.80367 1 AGAGTAC 23290 0.0 15.364553 10-11 CAACGCA 34855 0.0 15.0877285 5 GGTATCA 26290 0.0 15.076304 1 >>END_MODULE