FastQCFastQC Report
Fri 27 May 2016
SRR939008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2996279
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133220.4446181413680101No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111400.37179448242303204No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT108470.3620156867901821No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70200.2342905984389304No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51210.17091198783557873No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA48900.16320242540831478No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42240.1409748558128265No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA41020.13690313885989924No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37350.12465461327199504No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30960.10332816136281034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118950.024.2271021
TACATGG120750.023.1849062
GAGTACT116500.023.13657812-13
ACATGGG121000.021.84293
GTACTTT126550.021.16793414-15
AGTACTT121500.020.87604112-13
ACTTTTT147750.018.30637416-17
CATGGGG51650.018.006374
CATGGGA70650.017.9324474
GTATCAA335850.017.047851
TACTTTT152850.016.48563814-15
AGAGTAC239250.016.25450310-11
AGTACAT92000.016.2466262
CTTTGCG5600.016.0992662
ACCGTAA2709.581685E-615.816828
GGTATCA276700.015.64823151
ATCAACG369850.015.3955793
CAACGCA369250.015.3948965
TATCAAC371750.015.34242252
TCAACGC370750.015.3326114
Produced by FastQC (version 0.11.5)