##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939008_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2996279 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16015931760694 33.0 31.0 34.0 30.0 34.0 2 32.384468202059956 34.0 31.0 34.0 30.0 34.0 3 32.47701065221229 34.0 31.0 34.0 30.0 34.0 4 36.051397082848425 37.0 35.0 37.0 35.0 37.0 5 35.969666709942565 37.0 35.0 37.0 35.0 37.0 6 36.01374938715654 37.0 35.0 37.0 35.0 37.0 7 35.956586152357644 37.0 35.0 37.0 35.0 37.0 8 35.9289532116335 37.0 35.0 37.0 35.0 37.0 9 37.60094537257712 39.0 37.0 39.0 35.0 39.0 10-11 37.529522617887054 39.0 37.0 39.0 35.0 39.0 12-13 37.46151760233276 39.0 37.0 39.0 34.0 39.0 14-15 38.713393679293546 40.0 38.0 41.0 34.0 41.0 16-17 38.706441222596425 40.0 38.0 41.0 34.0 41.0 18-19 38.820427937451754 40.0 38.0 41.0 34.5 41.0 20-21 38.827004427825315 40.0 38.0 41.0 34.0 41.0 22-23 38.76520544315132 40.0 38.0 41.0 34.0 41.0 24-25 38.701718698425616 40.0 38.0 41.0 34.0 41.0 26-27 38.5430510643368 40.0 38.0 41.0 34.0 41.0 28-29 38.3673085850817 40.0 38.0 41.0 34.0 41.0 30-31 38.201707684765005 40.0 38.0 41.0 33.5 41.0 32-33 38.03037233849051 40.0 37.0 41.0 33.0 41.0 34-35 37.98299240491289 40.0 37.0 41.0 33.0 41.0 36-37 37.87450601229058 40.0 37.0 41.0 33.0 41.0 38-39 37.739919246505416 40.0 37.0 41.0 33.0 41.0 40-41 37.59164550430718 40.0 37.0 41.0 33.0 41.0 42-43 37.416549660428814 40.0 37.0 41.0 32.0 41.0 44-45 37.153464013197706 40.0 37.0 41.0 31.0 41.0 46-47 36.829165274662344 40.0 36.0 41.0 31.0 41.0 48-49 36.523407866890906 40.0 36.0 41.0 30.0 41.0 50-51 36.260709199643955 39.0 36.0 41.0 30.0 41.0 52-53 36.042403594591825 39.0 35.0 41.0 29.0 41.0 54-55 35.80495407804146 39.0 35.0 40.0 28.0 41.0 56-57 35.541113327563956 38.5 35.0 40.0 28.0 41.0 58-59 35.24807603030292 38.0 34.5 40.0 27.5 41.0 60-61 34.851863427938454 38.0 34.0 40.0 26.5 41.0 62-63 34.55601614535896 37.5 34.0 40.0 26.0 41.0 64-65 34.30588473236304 37.0 34.0 40.0 26.0 41.0 66-67 33.86192073568583 36.5 33.5 39.0 25.5 41.0 68-69 33.44208049383919 36.0 33.0 39.0 25.0 40.0 70-71 32.98404954945784 35.5 32.5 38.5 23.5 40.0 72-73 32.47424054969514 35.0 32.0 37.5 22.0 40.0 74-75 32.006089052454726 35.0 31.5 37.0 21.5 39.0 76-77 29.99987868285964 33.0 29.5 35.0 18.5 37.5 78-79 30.981791582159072 34.0 31.0 36.0 19.5 38.0 80-81 30.971944868952455 34.0 31.0 36.0 18.5 37.5 82-83 30.648834103900207 34.0 31.0 35.0 18.0 37.0 84-85 30.2511209737144 34.0 30.5 35.0 14.5 36.5 86-87 29.691811243211994 34.0 30.0 35.0 7.0 36.0 88-89 29.124361416276656 34.0 29.0 35.0 2.0 36.0 90-91 28.668133374762498 34.0 29.0 35.0 2.0 35.0 92-93 28.32575688045072 34.0 29.0 35.0 2.0 35.0 94-95 27.951426752982616 33.0 28.5 35.0 2.0 35.0 96-97 27.34847522543795 33.0 27.0 35.0 2.0 35.0 98-99 26.488913916227425 33.0 25.5 34.5 2.0 35.0 100-101 22.21196040155139 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19217191705737235 1101 2 0.17659005973610675 1101 3 0.2271907263255386 1101 4 0.08430503355715757 1101 5 0.1812953374933599 1101 6 0.15933309009185592 1101 7 0.16965076970818416 1101 8 0.21522161439832388 1101 9 0.25832509755170463 1101 10-11 0.26921351892043077 1101 12-13 0.24680297103023463 1101 14-15 0.3385439597506448 1101 16-17 0.35362116420935763 1101 18-19 0.3205966278562684 1101 20-21 0.36742593641742616 1101 22-23 0.3270560852191977 1101 24-25 0.38719431347026045 1101 26-27 0.3508326031581106 1101 28-29 0.41315614777399645 1101 30-31 0.38269348641772893 1101 32-33 0.409935776895189 1101 34-35 0.46562650411124196 1101 36-37 0.3881485110429779 1101 38-39 0.36349794353160547 1101 40-41 0.3963698186274769 1101 42-43 0.34168093736916916 1101 44-45 0.3408611989780326 1101 46-47 0.5179721854409749 1101 48-49 0.4021710687692348 1101 50-51 0.3616793350281782 1101 52-53 0.488548640284975 1101 54-55 0.4309957250937302 1101 56-57 0.3297634955985913 1101 58-59 0.3846881522042196 1101 60-61 0.5454437768489839 1101 62-63 0.4027742123822051 1101 64-65 0.2907278611267614 1101 66-67 0.41113025103684464 1101 68-69 0.47496196794404 1101 70-71 0.5453473811946168 1101 72-73 0.40318007890084573 1101 74-75 0.35467148899167 1101 76-77 0.1633543545476357 1101 78-79 0.390491229116833 1101 80-81 0.5269090694798706 1101 82-83 0.4542901237681569 1101 84-85 0.4514606941140933 1101 86-87 0.5232082204031911 1101 88-89 0.7236324541773946 1101 90-91 0.7071865248720712 1101 92-93 0.5727890360527432 1101 94-95 0.5814991893752044 1101 96-97 0.6237773367139035 1101 98-99 0.5576071205765487 1101 100-101 0.9928723178262757 1102 1 0.13197610891940315 1102 2 0.14219928335587895 1102 3 0.13353341569650468 1102 4 0.04974301378670276 1102 5 0.042208658027050205 1102 6 0.03874795065025438 1102 7 0.04144685380449431 1102 8 0.15152347737615202 1102 9 0.20938821848710631 1102 10-11 0.2570769998555491 1102 12-13 0.24069047578088032 1102 14-15 0.20437923241753708 1102 16-17 0.3044576013831133 1102 18-19 0.1313818137745173 1102 20-21 0.1637532967847548 1102 22-23 0.3002124532323762 1102 24-25 0.21173110787172078 1102 26-27 0.17800857944621384 1102 28-29 0.12842987942654105 1102 30-31 0.1798975948083168 1102 32-33 0.13615677983223406 1102 34-35 0.22924184327030162 1102 36-37 0.2430372896268267 1102 38-39 0.251549908776191 1102 40-41 0.16335400231852049 1102 42-43 0.08805522775184471 1102 44-45 0.15433945275140815 1102 46-47 0.20548188426763403 1102 48-49 0.1551222322786927 1102 50-51 0.2941084030884866 1102 52-53 0.3032013102440416 1102 54-55 0.18962246709366326 1102 56-57 0.20392920220555055 1102 58-59 0.1113945174583364 1102 60-61 -0.001095435685300572 1102 62-63 0.2797720411403688 1102 64-65 0.2919044146164893 1102 66-67 0.28872801456190444 1102 68-69 0.39595905007065824 1102 70-71 0.3862160929546832 1102 72-73 0.32762618684428446 1102 74-75 0.2519520092399645 1102 76-77 0.010885690663180014 1102 78-79 0.07607411596951152 1102 80-81 0.1693137515487706 1102 82-83 0.19014771247987383 1102 84-85 0.13973010737620584 1102 86-87 0.31080424040628785 1102 88-89 0.5819051031882907 1102 90-91 0.5541076930680582 1102 92-93 0.4400938671629788 1102 94-95 0.3489862871831555 1102 96-97 0.16126830498517108 1102 98-99 0.2958862099969437 1102 100-101 1.00166492757295 1103 1 0.04161527389608466 1103 2 0.13087659786459227 1103 3 0.1303765274283606 1103 4 0.09031495924863009 1103 5 0.0725601410098875 1103 6 0.06012739960514324 1103 7 0.1042637333437213 1103 8 0.07912484243696127 1103 9 0.14321716910490778 1103 10-11 0.1838940908499822 1103 12-13 0.13103926960516077 1103 14-15 0.1891589908427349 1103 16-17 0.11707930623813212 1103 18-19 0.15746135259787053 1103 20-21 0.18042773979311022 1103 22-23 0.09554999370702433 1103 24-25 0.16061730334570967 1103 26-27 0.08782989469246161 1103 28-29 0.18222064807896743 1103 30-31 0.22036515341754637 1103 32-33 0.1896124890333155 1103 34-35 0.14290133859211096 1103 36-37 0.10044082659815956 1103 38-39 0.18033681479584374 1103 40-41 0.18280922169946479 1103 42-43 0.16406801006560556 1103 44-45 0.10758755022919075 1103 46-47 0.2585409234284697 1103 48-49 0.12418880057271764 1103 50-51 0.03729644269190402 1103 52-53 0.08275932545604547 1103 54-55 0.09982921563027247 1103 56-57 0.03359431130545687 1103 58-59 0.07101940665504003 1103 60-61 -0.013345025352393236 1103 62-63 0.08082800194249984 1103 64-65 0.07481691889730513 1103 66-67 0.12035578802800018 1103 68-69 5.973250456747792E-4 1103 70-71 -0.029384997827271775 1103 72-73 0.029713323753611576 1103 74-75 0.07933510043411474 1103 76-77 -0.053380091160509124 1103 78-79 0.10189540108084572 1103 80-81 0.12028219339207524 1103 82-83 0.31576219031704866 1103 84-85 0.200618607405449 1103 86-87 0.1390554982199781 1103 88-89 0.4342551034308997 1103 90-91 0.4369599147395107 1103 92-93 0.45159729833009266 1103 94-95 0.2342031424101485 1103 96-97 0.19304078991324047 1103 98-99 0.3198820340120747 1103 100-101 0.7607449237831005 1104 1 -0.016507323358617043 1104 2 -0.04161723642391735 1104 3 0.017493711720625527 1104 4 -0.03751352116556461 1104 5 -1.371686134135075E-4 1104 6 -0.03033910038226395 1104 7 -0.09420386952886162 1104 8 0.034202464462957494 1104 9 0.006466512147817127 1104 10-11 -0.04004656973935283 1104 12-13 0.01764059830259157 1104 14-15 0.0017889207631540671 1104 16-17 0.05346970565258857 1104 18-19 0.027086812891639056 1104 20-21 0.03666551026984877 1104 22-23 -0.016981937984894557 1104 24-25 0.017998781206863157 1104 26-27 0.0010082257908621273 1104 28-29 -3.928557328904958E-4 1104 30-31 -0.026033829255638352 1104 32-33 0.1264364140630505 1104 34-35 0.04520799900643624 1104 36-37 -0.048238515769163826 1104 38-39 0.11097190613912034 1104 40-41 0.0656851344030116 1104 42-43 0.11026533726425924 1104 44-45 0.11220164387521692 1104 46-47 0.0035215369307834976 1104 48-49 0.1261472693672303 1104 50-51 0.027856229214812345 1104 52-53 -0.12309223577215533 1104 54-55 0.028328838071594475 1104 56-57 0.07402487528514001 1104 58-59 -0.015460726827967619 1104 60-61 -0.04069577998363627 1104 62-63 -0.06815024624007293 1104 64-65 -0.1750379908737827 1104 66-67 0.02194435152754437 1104 68-69 0.07086300220695563 1104 70-71 -0.28451014812155506 1104 72-73 -0.2357227683632317 1104 74-75 -0.18060407883882945 1104 76-77 -0.15379231443891683 1104 78-79 0.03142361851276121 1104 80-81 0.20290472171645746 1104 82-83 0.17983875956634776 1104 84-85 0.05744160006841881 1104 86-87 -0.18476588673356886 1104 88-89 -0.09562120590246437 1104 90-91 0.12200449009755232 1104 92-93 0.043084166542548985 1104 94-95 0.0981934552690582 1104 96-97 0.062000567760655656 1104 98-99 -0.11475494250699114 1104 100-101 0.386733226319663 1105 1 -0.03095480179179333 1105 2 0.019766181887952428 1105 3 0.008917726412647653 1105 4 -0.09041640399184558 1105 5 0.0014811287550742236 1105 6 -0.0773468088615914 1105 7 -0.11266459011461194 1105 8 -0.016565242083210308 1105 9 -0.15502250508147597 1105 10-11 -0.1762644611440365 1105 12-13 -0.11073252592716898 1105 14-15 -0.07146748887365106 1105 16-17 -0.1264268702360667 1105 18-19 -0.1319244960999768 1105 20-21 -0.05723440032146243 1105 22-23 -0.21117242683297377 1105 24-25 -0.09412576268797324 1105 26-27 -0.022199975644511483 1105 28-29 -0.1543705760528269 1105 30-31 -0.14010252896324715 1105 32-33 -0.23673155345984043 1105 34-35 -0.09698795102134738 1105 36-37 -0.11740556504059185 1105 38-39 -0.10599520596004908 1105 40-41 -0.009273544281469981 1105 42-43 -0.16868821854367866 1105 44-45 -0.16640998408795582 1105 46-47 -0.00631837317722983 1105 48-49 -0.151825932188963 1105 50-51 -0.1544146308001686 1105 52-53 0.011359713095735913 1105 54-55 0.05844168634262559 1105 56-57 0.03612924181245347 1105 58-59 -0.072693286091436 1105 60-61 -0.10740481509466804 1105 62-63 -0.17725434695775988 1105 64-65 -0.16344808680688772 1105 66-67 -0.09528651622009221 1105 68-69 -0.19144434622113948 1105 70-71 -0.16655676146520193 1105 72-73 -0.2793513114848025 1105 74-75 -0.15881735482172132 1105 76-77 -0.22050364517859222 1105 78-79 -0.042477137836463896 1105 80-81 -0.01413947517598757 1105 82-83 -0.10576039950863603 1105 84-85 -0.2605186734738574 1105 86-87 -0.2944174344705601 1105 88-89 -0.10434362815314557 1105 90-91 0.0592528771163785 1105 92-93 0.16491897793853028 1105 94-95 0.00811431649226435 1105 96-97 -0.04088866211634823 1105 98-99 0.12070080018928309 1105 100-101 0.5281369428216038 1106 1 0.0017289380265239629 1106 2 -0.10221006280114153 1106 3 -0.07732907530562727 1106 4 -0.09183280266793048 1106 5 -0.06403587788796017 1106 6 -0.0012678435790860476 1106 7 0.004154733375450803 1106 8 0.03400258496310471 1106 9 -0.07880833170526813 1106 10-11 0.039779411065538284 1106 12-13 0.023932270673853395 1106 14-15 0.04436993278311263 1106 16-17 -0.060048850940745524 1106 18-19 -0.0817283739432213 1106 20-21 -0.04312862815648799 1106 22-23 0.058203761519408204 1106 24-25 0.007347207430093761 1106 26-27 -0.018033993625309108 1106 28-29 0.0236550571482681 1106 30-31 0.02610800600071883 1106 32-33 -0.025461630218607922 1106 34-35 -0.01778171173118892 1106 36-37 -0.08741847553565663 1106 38-39 0.11721551679293896 1106 40-41 0.03129369592415543 1106 42-43 0.1287037440863088 1106 44-45 0.0805711900628836 1106 46-47 0.05119518284258362 1106 48-49 -0.05103827462224331 1106 50-51 -0.0439182809492209 1106 52-53 0.08918241678814098 1106 54-55 0.09497791788648868 1106 56-57 -0.014584473828023192 1106 58-59 0.0825292438961327 1106 60-61 0.1670814645701313 1106 62-63 0.07651516549500315 1106 64-65 -0.040191003639826306 1106 66-67 0.10452463844764992 1106 68-69 -0.036634482602913465 1106 70-71 -0.049756803717727394 1106 72-73 0.15971842866214558 1106 74-75 0.09709330455248022 1106 76-77 -0.01669727913084529 1106 78-79 -0.07989304356637916 1106 80-81 -0.015285457664660385 1106 82-83 0.09784749701961104 1106 84-85 0.08647552854018059 1106 86-87 0.08093455257199622 1106 88-89 0.010656489214991183 1106 90-91 0.2789413820810509 1106 92-93 0.3295845961165078 1106 94-95 0.4440492710707993 1106 96-97 0.35958679620622647 1106 98-99 0.2825947698144091 1106 100-101 0.8920751132870883 1107 1 -0.11070041122057717 1107 2 -0.07420604519647611 1107 3 -0.045251128246320604 1107 4 -0.03315285302620907 1107 5 -0.06527364106813849 1107 6 -0.021779972990188412 1107 7 -0.14496635801238966 1107 8 -0.01869138713251317 1107 9 -0.05829574155968231 1107 10-11 6.749752291526079E-4 1107 12-13 0.0423041156505235 1107 14-15 0.06655164876565323 1107 16-17 0.05885698529809247 1107 18-19 0.03513571744807109 1107 20-21 0.08042123886401953 1107 22-23 0.04096042805937827 1107 24-25 -0.01164720501250116 1107 26-27 -0.06425464627874078 1107 28-29 -0.008073825950724256 1107 30-31 0.0399176005659001 1107 32-33 0.08661519955958141 1107 34-35 -0.023825281087283656 1107 36-37 0.033450377546479615 1107 38-39 0.11991645749755975 1107 40-41 0.028394639393191312 1107 42-43 0.03324042373417058 1107 44-45 -0.11361197953693392 1107 46-47 -0.26131778971261355 1107 48-49 -0.2332866358472856 1107 50-51 -0.1335035508071698 1107 52-53 -0.03189105999486941 1107 54-55 -0.10852429767044924 1107 56-57 -0.17603862095666756 1107 58-59 -0.036978541940662524 1107 60-61 -0.1676440355859441 1107 62-63 -0.19003718142884196 1107 64-65 -0.23389414550342735 1107 66-67 -0.049948690209234314 1107 68-69 -0.2863305706216366 1107 70-71 -0.2796727542302335 1107 72-73 -0.15846094448555448 1107 74-75 -0.22697477357427331 1107 76-77 -0.4404380054096464 1107 78-79 -0.13042830184836873 1107 80-81 0.014107213662335027 1107 82-83 0.004561089493055448 1107 84-85 -0.07691111177247123 1107 86-87 -0.06400842577694021 1107 88-89 0.14260951963844093 1107 90-91 0.30952418816136174 1107 92-93 0.31688992140080785 1107 94-95 0.2675072215625711 1107 96-97 0.24344502129575218 1107 98-99 0.33377361657296944 1107 100-101 0.836951342728252 1108 1 -0.020373712308796144 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quality scores pass #Quality Count 2 1.0 3 7.0 4 31.0 5 64.0 6 163.0 7 392.0 8 977.0 9 1660.0 10 2868.0 11 4568.0 12 6671.0 13 9492.0 14 10950.0 15 14863.0 16 19363.0 17 27635.0 18 26901.0 19 17597.0 20 15330.0 21 16240.0 22 17639.0 23 19600.0 24 22698.0 25 27028.0 26 32160.0 27 38986.0 28 47656.0 29 58453.0 30 70806.0 31 86174.0 32 107225.0 33 135905.0 34 182492.0 35 260230.0 36 410917.0 37 686661.0 38 569825.0 39 46049.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.182167893093144 20.442451780664012 14.872062943155468 22.503317383087378 2 15.180695789677797 21.90243298437829 39.816986335384655 23.09988489055926 3 19.09485064641844 25.850062694428654 31.093299389008834 23.961787270144068 4 12.726084586915972 17.635874362834702 38.250276426193956 31.38776462405537 5 12.816021771046898 38.43501576792235 34.8319395462296 13.917022914801155 6 30.110513740542856 37.63004046018412 17.63260363938071 14.62684215989232 7 26.674885749958534 34.21183407820166 21.786288927032498 17.32699124480731 8 25.12910179592755 35.434183532307905 20.52806163911972 18.908653032644825 9 25.86217772110007 17.40525498459923 21.650620653150124 35.08194664115057 10-11 24.244771598372512 27.111393832149812 28.0044682087349 20.639366360742777 12-13 25.6451418576174 24.95410140377448 28.2049001444792 21.195856594128916 14-15 22.445227945996244 26.100647612537863 27.346727694347173 24.107396747118717 16-17 20.98515321386661 29.30107562769424 28.01905344228898 21.69471771615017 18-19 20.833724095786806 28.23246767073427 30.323294326062427 20.6105139074165 20-21 21.949107543055906 27.179928838402567 29.869932005664356 21.00103161287717 22-23 21.566599772584595 27.158619073857942 29.821071402229233 21.453709751328233 24-25 21.609019053299107 27.239736352989823 30.036722214453327 21.11452237925774 26-27 21.58018328733739 27.12342542199842 30.36556342049589 20.930827870168297 28-29 21.075507320913704 27.628351698890523 30.351095475421346 20.94504550477442 30-31 21.386442984782125 27.30663599751558 30.00274674020677 21.30417427749552 32-33 21.24304846110793 27.4791666597136 29.972225550424376 21.305559328754097 34-35 21.77063191510985 27.183318611915787 29.863645686046148 21.182403786928216 36-37 21.862832958673987 26.93271652222673 30.0599121177682 21.144538401331083 38-39 21.431695949864608 27.306148845709327 30.11923845605017 21.1429167483759 40-41 21.500529169752518 27.396491492732633 29.986219224798855 21.116760112715994 42-43 21.324820726625376 27.573806963476493 29.45471724435313 21.646655065545005 44-45 21.518187613265827 27.48675853644698 29.244226988889523 21.75082686139767 46-47 21.805661706054526 27.68393702837194 28.862552440200652 21.647848825372883 48-49 21.812227139819555 27.65340828877514 28.858189968924684 21.676174602480618 50-51 21.367012614211 27.98485513707322 28.902130937415887 21.74600131129989 52-53 21.685180185156323 28.261203312508616 28.39850694811798 21.65510955421708 54-55 21.349280223904383 27.678013295824588 28.717619420621375 22.255087059649654 56-57 21.69399111364462 28.06823062872316 28.319458902191684 21.918319355440534 58-59 21.97505969080264 27.71098370843254 28.438070083043137 21.875886517721675 60-61 21.673549802216932 27.713476215968697 28.8541832153399 21.75879076647447 62-63 21.532257176317692 27.72697402344708 28.834647908288915 21.90612089194631 64-65 21.368133238882645 27.899364960963997 28.735737487161135 21.996764312992227 66-67 21.40669629060024 27.822934585041313 28.336430562981562 22.43393856137688 68-69 21.172650286369016 27.93104581887554 28.586321138140153 22.30998275661529 70-71 21.48019259888682 27.840932036035365 28.336930572887237 22.341944792190578 72-73 21.47780630575457 27.53857367755139 28.46213586918975 22.521484147504285 74-75 21.134697403012204 28.23760737901911 28.050325086549016 22.57737013141967 76-77 21.260119058658336 27.6925246407046 28.37722303507183 22.670133265565234 78-79 21.290371350846403 27.78294530178618 28.277575804907222 22.649107542460193 80-81 21.25154232966957 27.985477987864282 28.08251501278753 22.680464669678624 82-83 21.221905570208914 27.835592079375786 28.27558782076035 22.66691452965495 84-85 21.19078363530232 27.700975109460767 28.02415929891709 23.08408195631982 86-87 21.114909638101174 27.648561753564653 28.23977715110253 22.996751457231642 88-89 21.207567574997146 27.52415508939348 28.010416486825935 23.257860848783434 90-91 20.9676865687762 27.40610923166496 28.32045610845302 23.305748091105816 92-93 21.21826772473458 27.75983144426804 27.94434363422098 23.077557196776404 94-95 20.986747228812806 27.482837879917057 27.96772263197119 23.562692259298952 96-97 20.666289864025643 27.409321643440236 28.139013808364986 23.785374684169135 98-99 20.799498311071833 27.503780522441335 27.685923106626586 24.010798059860246 100-101 20.796689887184826 27.050360072389946 28.32100551602032 23.831944524404907 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1563.0 1 1077.0 2 690.5 3 808.0 4 746.5 5 816.0 6 1263.0 7 2301.5 8 3596.0 9 4239.0 10 4008.0 11 3723.0 12 3884.0 13 4170.0 14 4436.5 15 4630.5 16 4849.5 17 5144.5 18 5507.0 19 5978.5 20 6694.5 21 7724.0 22 9283.0 23 11096.5 24 13008.5 25 15905.0 26 20978.0 27 26920.0 28 30942.5 29 35203.5 30 40427.5 31 45956.5 32 51622.0 33 58036.5 34 66310.5 35 73182.0 36 81629.5 37 89320.5 38 89420.0 39 88553.5 40 90661.5 41 93887.5 42 96642.0 43 99661.0 44 104344.5 45 107438.5 46 108291.5 47 116567.0 48 141791.0 49 141231.0 50 114522.0 51 100335.5 52 91681.5 53 87980.0 54 85367.5 55 79065.0 56 71844.5 57 63973.5 58 56131.0 59 49260.5 60 41657.5 61 32968.0 62 25480.0 63 19663.0 64 14698.0 65 10882.5 66 7700.5 67 5736.0 68 4111.5 69 2628.5 70 1802.0 71 1232.0 72 808.5 73 522.0 74 340.5 75 228.5 76 137.5 77 66.0 78 31.0 79 16.5 80 10.5 81 6.5 82 3.5 83 2.5 84 2.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4004633747391348 2 0.0 3 0.0 4 0.0 5 2.0024837473412856E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001435113352261255 16-17 0.0025031046841766068 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002586541506982494 36-37 0.007475939323407467 38-39 0.013066206451401888 40-41 0.002536479413298962 42-43 5.67370395080031E-4 44-45 3.003725621011929E-4 46-47 3.003725621011929E-4 48-49 3.337472912235476E-4 50-51 5.173083013964988E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.668736456117738E-4 60-61 0.002236106851197769 62-63 0.0 64-65 2.1693573929530593E-4 66-67 0.0017688606434848025 68-69 0.0019190469245353987 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12417067969972088 78-79 6.674945824470952E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14938528755165992 88-89 0.4444012056287148 90-91 0.344510641365507 92-93 0.0 94-95 0.0 96-97 5.173083013964988E-4 98-99 0.0 100-101 2.1693573929530593E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2996279.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.87085739061973 #Duplication Level Percentage of deduplicated Percentage of total 1 66.8077365458916 27.304894729572293 2 15.767186807343474 12.88836886908392 3 6.4716540988809985 7.935061552703547 4 3.286775368788631 5.373333094910468 5 1.9600796627523474 4.00550681853026 6 1.2650808757620806 3.102296403652339 7 0.8796634131413674 2.516681853717264 8 0.6028964938324332 1.971271729658402 9 0.45055582452309456 1.6573142565536836 >10 2.2422098977908633 16.176106441984746 >50 0.15960715607106712 4.547585068553793 >100 0.09936565587614515 7.518972678570755 >500 0.004329256424297581 1.2504680082230974 >1k 0.0024505225042668662 2.168020943312479 >5k 1.6336816695112442E-4 0.4053271061403882 >10k+ 2.450522504266866E-4 1.178790444832466 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13322 0.4446181413680101 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11140 0.37179448242303204 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10847 0.3620156867901821 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7020 0.2342905984389304 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5121 0.17091198783557873 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4890 0.16320242540831478 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4224 0.1409748558128265 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4102 0.13690313885989924 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3735 0.12465461327199504 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3096 0.10332816136281034 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.337472912235476E-5 0.0 8 0.0 0.0 0.0 3.337472912235476E-5 0.0 9 0.0 0.0 0.0 3.337472912235476E-5 0.0 10-11 0.0 0.0 0.0 3.337472912235476E-5 0.0 12-13 0.0 0.0 0.0 6.674945824470952E-5 0.0 14-15 0.0 0.0 0.0 6.674945824470952E-5 0.0 16-17 0.0 0.0 0.0 1.5018628105059644E-4 0.0 18-19 0.0 0.0 0.0 2.1693573929530593E-4 0.0 20-21 0.0 0.0 0.0 3.5043465578472497E-4 0.0 22-23 0.0 0.0 0.0 7.843061343753368E-4 0.0 24-25 0.0 0.0 0.0 0.001585299633311851 0.0 26-27 0.0 0.0 0.0 0.002636603600666026 0.0 28-29 0.0 0.0 0.0 0.007092129938500387 0.0 30-31 0.0 0.0 0.0 0.019257218703598696 0.0 32-33 0.0 0.0 0.0 0.04553981788745307 0.0 34-35 0.0 0.0 0.0 0.08642386106233765 0.0 36-37 0.0 0.0 0.0 0.14820048466781632 0.0 38-39 0.0 0.0 0.0 0.2413159789191861 0.0 40-41 0.0 0.0 0.0 0.34360952367920344 0.0 42-43 0.0 0.0 0.0 0.45242782798264114 0.0 44-45 0.0 0.0 0.0 0.5844415690261153 0.0 46-47 0.0 0.0 0.0 0.7424375366913428 0.0 48-49 0.0 0.0 0.0 0.9072085743684082 0.0 50-51 0.0 0.0 0.0 1.0784709968597717 0.0 52-53 0.0 0.0 0.0 1.2535882005647672 0.0 54-55 0.0 0.0 0.0 1.4532191428101322 0.0 56-57 0.0 0.0 0.0 1.6900128459332393 0.0 58-59 0.0 0.0 0.0 1.9499519237026992 0.0 60-61 0.0 0.0 0.0 2.23881020425668 0.0 62-63 0.0 0.0 0.0 2.5334423129488277 0.0 64-65 0.0 0.0 0.0 2.8384539623980274 0.0 66-67 0.0 0.0 0.0 3.1920592174493763 0.0 68-69 0.0 0.0 0.0 3.584395845647218 0.0 70-71 0.0 0.0 0.0 4.022756225304787 0.0 72-73 3.337472912235476E-5 0.0 0.0 4.469844096627851 0.0 74-75 3.337472912235476E-5 0.0 0.0 4.944582931028786 0.0 76-77 3.337472912235476E-5 0.0 0.0 5.447273101069694 0.0 78-79 3.337472912235476E-5 0.0 0.0 6.017897532239154 0.0 80-81 3.337472912235476E-5 0.0 0.0 6.638100123519873 0.0 82-83 3.337472912235476E-5 0.0 0.0 7.263642671460168 0.0 84-85 6.674945824470952E-5 0.0 0.0 7.914199578877668 0.0 86-87 6.674945824470952E-5 0.0 0.0 8.603421109983415 0.0 88-89 6.674945824470952E-5 0.0 0.0 9.327085361543435 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11895 0.0 24.227102 1 TACATGG 12075 0.0 23.184906 2 GAGTACT 11650 0.0 23.136578 12-13 ACATGGG 12100 0.0 21.8429 3 GTACTTT 12655 0.0 21.167934 14-15 AGTACTT 12150 0.0 20.876041 12-13 ACTTTTT 14775 0.0 18.306374 16-17 CATGGGG 5165 0.0 18.00637 4 CATGGGA 7065 0.0 17.932447 4 GTATCAA 33585 0.0 17.04785 1 TACTTTT 15285 0.0 16.485638 14-15 AGAGTAC 23925 0.0 16.254503 10-11 AGTACAT 9200 0.0 16.246626 2 CTTTGCG 560 0.0 16.099266 2 ACCGTAA 270 9.581685E-6 15.81682 8 GGTATCA 27670 0.0 15.6482315 1 ATCAACG 36985 0.0 15.395579 3 CAACGCA 36925 0.0 15.394896 5 TATCAAC 37175 0.0 15.3424225 2 TCAACGC 37075 0.0 15.332611 4 >>END_MODULE