##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939007_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2175407 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.861767935839133 33.0 31.0 34.0 30.0 34.0 2 31.912661860516216 33.0 31.0 34.0 30.0 34.0 3 31.963707480944944 34.0 31.0 34.0 30.0 34.0 4 35.487807109198414 37.0 35.0 37.0 33.0 37.0 5 35.54618607000897 37.0 35.0 37.0 33.0 37.0 6 35.65346024904765 37.0 35.0 37.0 33.0 37.0 7 35.5894924489992 37.0 35.0 37.0 33.0 37.0 8 35.59137117789913 37.0 35.0 37.0 33.0 37.0 9 37.1898601043391 39.0 37.0 39.0 34.0 39.0 10-11 37.08052240339394 39.0 37.0 39.0 33.0 39.0 12-13 36.992068840451466 39.0 37.0 39.0 33.0 39.0 14-15 38.13833733181883 40.0 38.0 41.0 33.0 41.0 16-17 38.092337663710744 40.0 38.0 41.0 33.0 41.0 18-19 38.073900424150516 40.0 38.0 41.0 33.0 41.0 20-21 38.179123492753305 40.0 38.0 41.0 33.0 41.0 22-23 38.11560319517221 40.0 38.0 41.0 33.0 41.0 24-25 38.02827264047601 40.0 38.0 41.0 33.0 41.0 26-27 37.883762440775456 40.0 38.0 41.0 33.0 41.0 28-29 37.771193160636145 40.0 37.5 41.0 33.0 41.0 30-31 37.587840344358554 40.0 37.0 41.0 32.0 41.0 32-33 37.36177437141647 40.0 36.5 41.0 31.5 41.0 34-35 37.01696762950565 40.0 36.0 41.0 31.0 41.0 36-37 36.723170882506125 39.5 35.5 41.0 30.0 41.0 38-39 36.463516482203104 39.0 35.0 41.0 30.0 41.0 40-41 36.34391863223755 39.0 35.0 41.0 30.0 41.0 42-43 36.120135220673646 39.0 35.0 41.0 30.0 41.0 44-45 35.999578239842016 39.0 35.0 41.0 29.0 41.0 46-47 35.720671350234696 39.0 35.0 41.0 27.5 41.0 48-49 35.4209292789809 39.0 35.0 41.0 26.0 41.0 50-51 34.26841437027646 38.0 34.0 40.0 24.0 40.5 52-53 34.391251384223736 38.0 34.0 39.5 24.0 40.5 54-55 34.91363110443241 39.0 35.0 40.0 24.0 41.0 56-57 34.81767342846649 39.0 35.0 41.0 24.0 41.0 58-59 34.62975663864279 38.5 34.5 40.0 23.5 41.0 60-61 34.27826172297874 38.0 34.0 40.0 22.5 41.0 62-63 33.93788035985909 38.0 34.0 40.0 22.0 41.0 64-65 33.55639427472652 37.0 33.5 40.0 21.5 41.0 66-67 33.11228105821118 36.5 33.0 39.0 19.5 41.0 68-69 32.66700805872189 36.0 32.5 39.0 18.0 41.0 70-71 32.17341398644024 35.5 32.0 38.5 14.0 40.0 72-73 31.71768914966257 35.0 31.5 37.5 10.5 40.0 74-75 31.26996557425806 35.0 31.0 37.0 7.5 39.0 76-77 30.851832093948396 35.0 31.0 36.5 6.5 39.0 78-79 30.448764300197617 35.0 30.5 36.0 2.0 39.0 80-81 29.978970831665066 34.0 30.0 36.0 2.0 37.5 82-83 29.508309479559458 34.0 29.5 35.0 2.0 37.0 84-85 29.076627270207368 34.0 29.0 35.0 2.0 36.5 86-87 28.668283682087996 34.0 29.0 35.0 2.0 36.0 88-89 28.161941190774876 34.0 28.5 35.0 2.0 36.0 90-91 27.67081447287795 34.0 27.0 35.0 2.0 35.0 92-93 27.183154462590217 33.5 26.0 35.0 2.0 35.0 94-95 26.642541602559888 33.0 25.0 35.0 2.0 35.0 96-97 25.981587123696855 33.0 24.0 35.0 2.0 35.0 98-99 25.181304693788334 32.5 18.5 35.0 2.0 35.0 100-101 22.104052483052598 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18391384273600764 1101 2 0.2695424063674672 1101 3 0.4406821258607536 1101 4 0.45042656830346317 1101 5 0.202764405047688 1101 6 0.3487986739775053 1101 7 0.33724092660142446 1101 8 0.3102656289141663 1101 9 0.40430652065833783 1101 10-11 0.27019197640397863 1101 12-13 0.16775604843200398 1101 14-15 0.3845572094748846 1101 16-17 0.3376249472965469 1101 18-19 0.477739238758609 1101 20-21 0.46769879345262666 1101 22-23 0.489828212436187 1101 24-25 0.44978864410634856 1101 26-27 0.5368451189197287 1101 28-29 0.3412882754112374 1101 30-31 0.3967362850786813 1101 32-33 0.4741755279086206 1101 34-35 0.3751873634854732 1101 36-37 0.4536917059334584 1101 38-39 0.5807918791718762 1101 40-41 0.4125374416314287 1101 42-43 0.4687144213636927 1101 44-45 0.5210784127612484 1101 46-47 0.5388967885338332 1101 48-49 0.5290962599821327 1101 50-51 0.5581965300464091 1101 52-53 0.5704591689917962 1101 54-55 0.5371879320405952 1101 56-57 0.6103852528964282 1101 58-59 0.507636303162947 1101 60-61 0.5280224785799987 1101 62-63 0.4946835544532462 1101 64-65 0.3719115462604208 1101 66-67 0.4279086792270945 1101 68-69 0.4173101337118936 1101 70-71 0.29706746268160344 1101 72-73 0.19860300487722782 1101 74-75 0.09213877484033972 1101 76-77 0.0192626505952731 1101 78-79 -0.11579440049001022 1101 80-81 -0.12771868961873523 1101 82-83 -0.05759705778075386 1101 84-85 -0.06468070980904983 1101 86-87 -0.1727539980737447 1101 88-89 -0.35332626490607666 1101 90-91 -0.2885200380246431 1101 92-93 -0.4329374100172423 1101 94-95 -0.6561021810191718 1101 96-97 -0.6112070692163023 1101 98-99 -0.6243464889084187 1101 100-101 -0.3536520752211345 1102 1 0.299010728713764 1102 2 0.3662035395540819 1102 3 0.2849659908838973 1102 4 0.26527482325417395 1102 5 0.36958681038386487 1102 6 0.31470868106336525 1102 7 0.30200743126983554 1102 8 0.299652113702102 1102 9 0.4504397197859973 1102 10-11 0.4503837452897912 1102 12-13 0.38762637470701833 1102 14-15 0.5883898329703854 1102 16-17 0.6387512984296606 1102 18-19 0.5981129614887024 1102 20-21 0.5287060370534462 1102 22-23 0.574638934868311 1102 24-25 0.6575357665715202 1102 26-27 0.6589476159965031 1102 28-29 0.6442034929821645 1102 30-31 0.6197623224810016 1102 32-33 0.6653759175056777 1102 34-35 0.5972916132482311 1102 36-37 0.5631211565665524 1102 38-39 0.7471123903552694 1102 40-41 0.772933623780574 1102 42-43 0.7227387135915322 1102 44-45 0.8386674930267546 1102 46-47 0.8591050076296014 1102 48-49 0.6273578458624911 1102 50-51 0.627714816820891 1102 52-53 0.7348323208470191 1102 54-55 0.7610714084682186 1102 56-57 0.7523162621681436 1102 58-59 0.7278579177873539 1102 60-61 0.6927516760751615 1102 62-63 0.6289549503674863 1102 64-65 0.6699998666113558 1102 66-67 0.8364014443897005 1102 68-69 0.8427320928642956 1102 70-71 0.6603811158550883 1102 72-73 0.6252222989453919 1102 74-75 0.4943593027994382 1102 76-77 0.47706060095419645 1102 78-79 0.49869467902434295 1102 80-81 0.4995905367707074 1102 82-83 0.514551509899217 1102 84-85 0.3831276815507074 1102 86-87 0.25411167356804754 1102 88-89 0.15394487812061186 1102 90-91 0.22041515515890708 1102 92-93 0.2362706513367847 1102 94-95 0.27767282918396674 1102 96-97 0.119053805574314 1102 98-99 -0.21188514189411833 1102 100-101 0.2763169773519323 1103 1 0.30751056822588296 1103 2 0.31017471191806223 1103 3 0.4554517676452505 1103 4 0.36821375761463315 1103 5 0.2623949471195388 1103 6 0.27322808003624033 1103 7 0.20460282125538498 1103 8 0.3338455617842726 1103 9 0.41986176279674936 1103 10-11 0.43735413527534917 1103 12-13 0.4004147513720895 1103 14-15 0.581467037672688 1103 16-17 0.46660697970072107 1103 18-19 0.6179949025896505 1103 20-21 0.549387659184724 1103 22-23 0.5414716061891269 1103 24-25 0.5148369622062532 1103 26-27 0.5685382715895031 1103 28-29 0.5199550276475478 1103 30-31 0.5651302409531596 1103 32-33 0.6244442351914401 1103 34-35 0.5368877354597572 1103 36-37 0.506694870657391 1103 38-39 0.5244517520147198 1103 40-41 0.3756732020986675 1103 42-43 0.32903685995648146 1103 44-45 0.3664158480259445 1103 46-47 0.2575951677162607 1103 48-49 0.3204265246459954 1103 50-51 0.350103628809336 1103 52-53 0.41749260812032674 1103 54-55 0.3325504547689775 1103 56-57 0.374950821227678 1103 58-59 0.4320328596105014 1103 60-61 0.36447897707018484 1103 62-63 0.3091414673791988 1103 64-65 0.15988988225893053 1103 66-67 0.22664177499473936 1103 68-69 0.286167636918222 1103 70-71 0.31947977578126796 1103 72-73 0.2725968174817375 1103 74-75 0.2691112988674824 1103 76-77 0.24464129626795028 1103 78-79 0.08910853715305578 1103 80-81 0.1714535001794637 1103 82-83 0.1857835553172862 1103 84-85 -0.07145851047304674 1103 86-87 0.1639395503133514 1103 88-89 0.26812699174603694 1103 90-91 0.10496744411734582 1103 92-93 0.06417372668584065 1103 94-95 0.06060619662097366 1103 96-97 -0.08795525196059373 1103 98-99 0.023934499816679278 1103 100-101 0.33602373589791057 1104 1 0.21903578933036627 1104 2 0.25608432896349953 1104 3 0.33209030979504206 1104 4 0.24373453263982015 1104 5 0.20173815085552604 1104 6 0.21212072841214535 1104 7 0.12233760982672237 1104 8 0.24589980435148107 1104 9 0.22898871995126058 1104 10-11 0.29820524759482936 1104 12-13 0.30288313503034203 1104 14-15 0.4020629152535662 1104 16-17 0.4176926107081229 1104 18-19 0.44369245124863 1104 20-21 0.4400800050912963 1104 22-23 0.4798604721990145 1104 24-25 0.46537682898380694 1104 26-27 0.3961449954847822 1104 28-29 0.4770104547030627 1104 30-31 0.4604764513902424 1104 32-33 0.438562661031483 1104 34-35 0.4296566842738656 1104 36-37 0.5775971158874498 1104 38-39 0.5576327928050944 1104 40-41 0.6167449341104287 1104 42-43 0.6641545890974925 1104 44-45 0.681219852695925 1104 46-47 0.8031626075822444 1104 48-49 0.6726299977456165 1104 50-51 0.5711182754819717 1104 52-53 0.6316176061211891 1104 54-55 0.6884063500222268 1104 56-57 0.684762607535994 1104 58-59 0.7468392382549283 1104 60-61 0.7656045658158419 1104 62-63 0.7260444902733099 1104 64-65 0.693929302229499 1104 66-67 0.7931220555135567 1104 68-69 0.8644430719302889 1104 70-71 0.7057634012550622 1104 72-73 0.6004189483212166 1104 74-75 0.49018791876768475 1104 76-77 0.5388400773945037 1104 78-79 0.48471413553455633 1104 80-81 0.3633193641262409 1104 82-83 0.6089020181413467 1104 84-85 0.7379824360380631 1104 86-87 0.8252628586441517 1104 88-89 0.6669352065972163 1104 90-91 0.6701851450705298 1104 92-93 0.56573142875855 1104 94-95 0.4262011346077621 1104 96-97 0.34599865495162163 1104 98-99 0.517252511435661 1104 100-101 0.40357742770305194 1105 1 0.08450852147460708 1105 2 0.13330482879625905 1105 3 0.0774026317852261 1105 4 0.14909579677949125 1105 5 0.13048321030488808 1105 6 0.12428963469793786 1105 7 0.13673726080905624 1105 8 0.24554903770530956 1105 9 0.1625089668870956 1105 10-11 0.2037074013846265 1105 12-13 0.1691242015941441 1105 14-15 0.21035643309080143 1105 16-17 0.1633445253377417 1105 18-19 0.2556371972428053 1105 20-21 0.18412565625288835 1105 22-23 0.16754066823967406 1105 24-25 0.2013782884062465 1105 26-27 0.03619550199521626 1105 28-29 0.11368070271985431 1105 30-31 0.061760806299048454 1105 32-33 0.044290141096205105 1105 34-35 0.22690530373748174 1105 36-37 0.18894451405642343 1105 38-39 0.3409675876661211 1105 40-41 0.35766028445076614 1105 42-43 0.24709025329624268 1105 44-45 0.35709483207867265 1105 46-47 0.4623869809087253 1105 48-49 0.40538905190967256 1105 50-51 0.230863697600725 1105 52-53 0.2861582487771841 1105 54-55 0.38922790672168617 1105 56-57 0.2940375918255853 1105 58-59 0.15778337623738992 1105 60-61 0.4025768763842521 1105 62-63 0.37394813729906673 1105 64-65 0.41660417968586927 1105 66-67 0.516661096642892 1105 68-69 0.5176829144545323 1105 70-71 0.21897411362956376 1105 72-73 0.29367060128474876 1105 74-75 0.3059835675608831 1105 76-77 0.3116574598737074 1105 78-79 0.30263102375829476 1105 80-81 0.3367412603697595 1105 82-83 0.2968329005939516 1105 84-85 0.5433784853465262 1105 86-87 0.5589951445034238 1105 88-89 0.5357777519679345 1105 90-91 0.651595120106343 1105 92-93 0.5918872279344392 1105 94-95 0.7015217375085854 1105 96-97 0.7281516285612923 1105 98-99 0.8137178573185366 1105 100-101 0.7836813070995667 1106 1 0.19417038669761055 1106 2 0.1916121429250559 1106 3 0.23354533749684236 1106 4 0.1848840016965667 1106 5 0.2773970935687018 1106 6 0.2310944329124851 1106 7 0.29731984259722566 1106 8 0.21876330678610856 1106 9 0.1972711519786472 1106 10-11 0.14503075737764703 1106 12-13 0.2793531022858602 1106 14-15 0.33555774814735884 1106 16-17 0.33115453888139257 1106 18-19 0.3622818981740963 1106 20-21 0.33996225595891616 1106 22-23 0.42639701708082356 1106 24-25 0.3289812955804692 1106 26-27 0.33067145875987336 1106 28-29 0.3248820867055642 1106 30-31 0.16733955040979964 1106 32-33 0.3714229163517544 1106 34-35 0.3722272960509514 1106 36-37 0.2751399598883495 1106 38-39 0.3285930573624398 1106 40-41 0.4711911479468114 1106 42-43 0.35259797243042357 1106 44-45 0.3289066489581174 1106 46-47 0.4443122887146629 1106 48-49 0.46066918838940296 1106 50-51 0.3549628447421256 1106 52-53 0.44852029731448795 1106 54-55 0.4930004041741043 1106 56-57 0.41336747000726604 1106 58-59 0.4707503945669558 1106 60-61 0.6182515531158117 1106 62-63 0.5222522471535171 1106 64-65 0.4604991288552682 1106 66-67 0.5211729291711222 1106 68-69 0.4996684188389864 1106 70-71 0.5065849824536528 1106 72-73 0.4622221854444497 1106 74-75 0.4265370547242249 1106 76-77 0.18970082190268656 1106 78-79 0.35232836421058167 1106 80-81 0.45486336464653476 1106 82-83 0.47575925444644085 1106 84-85 0.48875992905841414 1106 86-87 0.566314442990894 1106 88-89 0.6069554904581622 1106 90-91 0.44057339438105103 1106 92-93 0.5887016995179302 1106 94-95 0.5537231857660743 1106 96-97 0.6394428300337651 1106 98-99 0.743814260334279 1106 100-101 0.9306371067429993 1107 1 0.12609699549307507 1107 2 0.08310722865573794 1107 3 0.06249110118147172 1107 4 0.10461820070653971 1107 5 0.10310553750298368 1107 6 0.012943919963205985 1107 7 0.0031972369583712634 1107 8 0.037453671948604494 1107 9 0.11113154368536726 1107 10-11 -0.06965286411600147 1107 12-13 0.20083509525522203 1107 14-15 0.24045497893866496 1107 16-17 0.12555783180054192 1107 18-19 0.16483947045330893 1107 20-21 0.22722640690231088 1107 22-23 0.1875097224398914 1107 24-25 0.21607326774490332 1107 26-27 0.10918229167471338 1107 28-29 0.12569814237669874 1107 30-31 0.2667127120426258 1107 32-33 0.0747723316613147 1107 34-35 0.18616211920899417 1107 36-37 0.21657675592823722 1107 38-39 0.3634305636397883 1107 40-41 0.1902796765356669 1107 42-43 0.23152849111898632 1107 44-45 -0.06954732908908312 1107 46-47 0.1687114565172365 1107 48-49 0.3050554427835479 1107 50-51 0.2456006236358732 1107 52-53 0.3239328089724296 1107 54-55 0.386967869558994 1107 56-57 0.4155824782575053 1107 58-59 0.3159903687401382 1107 60-61 0.4038952506550899 1107 62-63 0.3461555042020805 1107 64-65 0.09841791786453058 1107 66-67 0.146415055579034 1107 68-69 0.2930700258409189 1107 70-71 0.1737212547830893 1107 72-73 0.3001131747550154 1107 74-75 0.35918376539213526 1107 76-77 0.2871692557932093 1107 78-79 0.4433804482912187 1107 80-81 0.21054167729450057 1107 82-83 0.2753700586648229 1107 84-85 0.30001611673328554 1107 86-87 0.37984034154889557 1107 88-89 0.46049232954274544 1107 90-91 0.43517038842653477 1107 92-93 0.5646711019140831 1107 94-95 0.6015592930908475 1107 96-97 0.6552231944788502 1107 98-99 0.6313608920869171 1107 100-101 0.6839838317591393 1108 1 0.16752893453659468 1108 2 0.2949126746459605 1108 3 0.2695169204466126 1108 4 0.2377802254121022 1108 5 0.14060310128547826 1108 6 0.19973730087625086 1108 7 0.2729818753308848 1108 8 0.2322202127053643 1108 9 0.21072829168303286 1108 10-11 0.337024692056211 1108 12-13 0.27959997938408065 1108 14-15 0.27309982947091527 1108 16-17 0.3440331772793854 1108 18-19 0.4090734076592213 1108 20-21 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-1.6677819071453044 2316 68-69 -1.8376521899897256 2316 70-71 -1.6222491777985937 2316 72-73 -1.696147035783408 2316 74-75 -1.7891228671435293 2316 76-77 -1.8136051632127739 2316 78-79 -1.7387582999830578 2316 80-81 -1.5921123436442954 2316 82-83 -1.3654433329596607 2316 84-85 -1.4647266064604558 2316 86-87 -1.327289059049356 2316 88-89 -1.5530337576638793 2316 90-91 -1.7520779433728215 2316 92-93 -1.6637594951866994 2316 94-95 -1.7872989961406311 2316 96-97 -2.1271283931879736 2316 98-99 -1.9312189226852858 2316 100-101 -1.7589248773987052 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5703.0 3 1408.0 4 1398.0 5 1643.0 6 1855.0 7 2318.0 8 2882.0 9 3697.0 10 4908.0 11 6704.0 12 9890.0 13 14297.0 14 13225.0 15 14122.0 16 18206.0 17 25098.0 18 20772.0 19 13754.0 20 13835.0 21 14183.0 22 15750.0 23 17296.0 24 19819.0 25 22722.0 26 26049.0 27 30455.0 28 35815.0 29 43115.0 30 51471.0 31 63018.0 32 78377.0 33 100247.0 34 134447.0 35 191104.0 36 282571.0 37 423474.0 38 388495.0 39 61269.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.44931343107379 19.049981922688133 13.984230312331677 21.516474333906395 2 14.899495163962445 22.17234540115211 39.29674449076338 23.63141494412206 3 21.383696993511446 25.642666720653583 30.58771509262591 22.38592119320906 4 12.246549763787716 16.282933863781412 39.321626480439264 32.14888989199161 5 12.366555821751067 39.170761608573564 34.4912071112551 13.97147545842027 6 29.586995725860866 36.85758557361909 17.699848084778168 15.855570615741875 7 26.26399772237331 34.66927729047053 21.59578289507877 17.470942092077383 8 24.913208498300047 32.88597611232945 20.713502494738236 21.487312894632264 9 25.522942691911236 18.259631266584517 22.43305363022983 33.78437241127441 10-11 24.5793156040789 26.119906695235485 27.73344531499557 21.56733238569005 12-13 27.482503877295333 24.312113535302245 27.41243199785116 20.792950589551264 14-15 23.10204763079773 25.635617337424566 28.592115560140545 22.67021947163716 16-17 20.613769311948346 28.771764569524166 28.84374491911869 21.770721199408797 18-19 20.234353276904983 28.918106066373188 30.467282927454164 20.380257729267665 20-21 22.21015779383029 26.496497661427725 29.48539910284198 21.807945441900003 22-23 20.988874614268973 26.921823261291873 29.820910388485633 22.268391735953525 24-25 22.215182739003108 26.659282110740733 30.007085492913355 21.118449657342804 26-27 22.009405711241353 25.70668203920457 31.567860844793344 20.716051404760734 28-29 20.502210146061024 28.277746902391044 30.54362968183834 20.676413269709588 30-31 21.08170448870836 27.046476499531114 30.063100501919603 21.808718509840922 32-33 20.967576484095982 27.23067496628205 30.344203731882924 21.457544817739045 34-35 22.29962963569276 26.51087217416507 29.980832730585387 21.208665459556787 36-37 21.87877796764339 26.105736205062254 31.139230623891006 20.876255203403353 38-39 21.537469299384 26.961444921778664 30.830253650128896 20.670832128708437 40-41 21.335314580289104 27.45147564957141 30.285442244657983 20.927767525481503 42-43 20.60122481472697 27.892676169600605 29.209870481512418 22.296228534160008 44-45 21.439570508291446 26.90868843489084 29.19022455511304 22.46151650170467 46-47 22.254905040195556 27.67797919180236 28.783957437609587 21.283158330392496 48-49 22.00136380394117 27.30599288345667 29.006797768018426 21.685845544583728 50-51 20.82382822713947 27.94101613858468 30.07392501060229 21.161230623673557 52-53 21.56313318667348 28.72915479020588 28.264321279322346 21.443390743798293 54-55 20.550570299349417 27.33940063598586 29.265050156409657 22.84497890825507 56-57 21.88400000738936 27.417128347610188 28.396241402943552 22.3026302420569 58-59 22.907543095884996 27.066736977035845 28.18710069466891 21.83861923241025 60-61 21.610458859448517 27.24439462452622 29.91837641840792 21.226770097617344 62-63 21.495453583876596 27.335563662296845 29.490088230865375 21.67889452296118 64-65 20.979446146909066 28.626630797099946 28.87331491807149 21.5206081379195 66-67 21.326232690218735 27.422306683281654 28.2339275640146 23.01753306248501 68-69 20.792211431979073 27.81637707375106 28.78163031380839 22.609781180461482 70-71 22.019582265337892 27.469073763193126 28.124103265050937 22.387240706418048 72-73 21.583562483092432 26.569166649532892 29.47469241799411 22.372578449380562 74-75 20.78233056608307 28.83072028979313 28.29914025419275 22.08780888993105 76-77 21.3034208613578 27.091721618523597 28.335226074607036 23.26963144551157 78-79 21.32257092417628 27.20204687444074 29.031925837748716 22.443456363634265 80-81 21.856321235207382 27.463989090787326 28.469881312661872 22.209808361343423 82-83 21.41929207917705 26.993081350084797 29.205053891656856 22.382572679081296 84-85 21.150660722138255 27.341997612827175 28.795272215303775 22.712069449730794 86-87 21.242223745498688 27.55477790444376 28.63042767321162 22.57257067684593 88-89 21.295185293569435 27.20297469344088 28.136873961939575 23.36496605105011 90-91 21.357195888024386 26.34560664391476 28.804896449861335 23.492301018199523 92-93 21.88405576131231 27.340013343387078 28.613156357883685 22.162774537416926 94-95 21.521666707482005 26.96175849116933 28.56429963880735 22.952275162541312 96-97 20.339503413538136 26.78788290407973 29.497090759479605 23.37552292290253 98-99 21.36579880013704 27.241218473795936 28.00385615782802 23.389126568239 100-101 21.4816457238411 25.904109921252207 29.08113713710939 23.5331072177973 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2975.0 1 1841.5 2 835.5 3 994.5 4 877.5 5 884.5 6 1437.0 7 2580.0 8 3821.0 9 4313.0 10 3929.5 11 3559.5 12 3627.0 13 3839.0 14 4149.5 15 4509.0 16 4888.0 17 5077.0 18 5179.0 19 5502.5 20 5938.5 21 6524.5 22 7534.0 23 8996.5 24 10675.0 25 12826.5 26 15802.0 27 18945.0 28 21360.5 29 24096.5 30 27501.5 31 31250.0 32 34791.5 33 38341.5 34 42439.0 35 46080.0 36 50367.5 37 54911.0 38 56900.0 39 57020.5 40 58164.0 41 60541.0 42 62543.5 43 64681.5 44 67817.0 45 71257.5 46 77809.5 47 101524.0 48 157046.5 49 154419.5 50 96303.0 51 71341.0 52 62946.5 53 58944.5 54 55449.0 55 50921.0 56 45432.5 57 39778.5 58 35641.5 59 31603.5 60 26723.0 61 22061.5 62 17679.5 63 13359.0 64 9620.0 65 7346.0 66 5252.0 67 3821.0 68 2772.0 69 1809.5 70 1354.0 71 994.0 72 714.5 73 575.0 74 438.5 75 355.0 76 261.5 77 125.5 78 77.5 79 54.0 80 41.0 81 39.5 82 27.5 83 19.0 84 17.0 85 17.0 86 12.0 87 7.5 88 7.5 89 6.5 90 5.5 91 4.5 92 2.0 93 0.0 94 1.5 95 3.0 96 2.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31915866778032803 2 0.0841221895488982 3 0.09382152397229576 4 0.09883208061755801 5 0.11037015142453803 6 0.05369110240060825 7 0.0558056492417281 8 0.056587112204750656 9 0.06049442701986341 10-11 0.05706978050544105 12-13 0.05566774401295941 14-15 0.053438276147865664 16-17 0.05527701253144814 18-19 0.06350535784797971 20-21 0.06578079412266302 22-23 0.08157094281667752 24-25 0.08973033551882476 26-27 0.08430606318725646 28-29 0.08251329521326355 30-31 0.07596279684675097 32-33 0.06853889869803673 34-35 0.31564208444672653 36-37 0.492689413980924 38-39 0.4661656416477468 40-41 0.44899644066604544 42-43 0.47928962258556673 44-45 0.4879776519979939 46-47 0.48225458500409346 48-49 0.49981451746730615 50-51 0.4955854237850664 52-53 0.49466605559327514 54-55 0.48629980504797493 56-57 0.4657059575518512 58-59 0.44915733009960895 60-61 0.4108426607067091 62-63 0.4783013017793911 64-65 0.5009637277070451 66-67 0.5100654728057784 68-69 0.5223620223709863 70-71 0.5197188388195864 72-73 0.5086634363132968 74-75 0.5104562042872897 76-77 0.4853114842417993 78-79 0.4667862151772059 80-81 0.4718427402320577 82-83 0.48089851692120145 84-85 0.48280620591916823 86-87 0.481886837727377 88-89 0.48783974676922526 90-91 0.5010556645262243 92-93 0.5074452734591733 94-95 0.5142945664880181 96-97 0.5061351737858708 98-99 0.509169088818782 100-101 0.4865526313007175 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2175407.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.864499169300196 #Duplication Level Percentage of deduplicated Percentage of total 1 72.45390279400141 35.40423672890039 2 14.63157650736227 14.299293161791118 3 5.536661794867773 8.116386170280373 4 2.607308841713228 5.096193629200204 5 1.4050303007419975 3.432805098172447 6 0.849163641925134 2.4896373645270384 7 0.542934271428281 1.8571147878634293 8 0.3888022896277886 1.5198903326831275 9 0.2720792079683887 1.1965512808579668 >10 1.205536930709739 10.03363663513888 >50 0.06544205347747324 2.2163407486385895 >100 0.0349518454252507 2.991761042971423 >500 0.001982856225433834 0.7108589977119928 >1k 0.0031159169256817394 3.5245816638032603 >5k 0.0010386389752272463 3.4811651442441653 >10k+ 4.7210862510329383E-4 3.6295472132155266 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 28843 1.3258668377917326 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15313 0.7039142560449607 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12143 0.5581943976460496 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11616 0.5339690457923506 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10751 0.49420637149737956 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8818 0.40534943576075644 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8481 0.38985808172907416 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7975 0.36659806647675586 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7106 0.32665151854342656 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6948 0.3193885098282758 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6727 0.30922949130898264 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6292 0.28923323313752325 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6072 0.2791201830278196 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5770 0.2652377233317719 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5653 0.2598594194097932 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5608 0.25779084097826294 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4675 0.2149023148312017 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4365 0.20065210785843754 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3950 0.18157521787876935 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3679 0.1691177788799981 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3585 0.16479674837857927 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3454 0.15877488672234666 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3394 0.15601678214697295 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3131 0.1439270904249182 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3065 0.1408931753920071 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2813 0.12930913617543752 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2762 0.12696474728636986 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2682 0.12328727451920492 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2596 0.11933399129450259 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2426 0.11151936166427708 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2350 0.10802576253547039 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2218 0.1019579324696482 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2192 0.1007627538203196 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.596840958956186E-5 0.0 3 0.0 0.0 0.0 4.596840958956186E-5 0.0 4 0.0 0.0 0.0 4.596840958956186E-5 0.0 5 0.0 0.0 0.0 4.596840958956186E-5 0.0 6 0.0 0.0 0.0 4.596840958956186E-5 0.0 7 0.0 0.0 0.0 9.193681917912373E-5 0.0 8 0.0 0.0 0.0 9.193681917912373E-5 0.0 9 0.0 0.0 0.0 9.193681917912373E-5 0.0 10-11 0.0 0.0 0.0 1.608894335634665E-4 0.0 12-13 0.0 0.0 0.0 3.677472767164949E-4 2.298420479478093E-5 14-15 0.0 0.0 0.0 3.677472767164949E-4 4.596840958956186E-5 16-17 0.0 0.0 0.0 5.516209150747424E-4 4.596840958956186E-5 18-19 0.0 0.0 0.0 6.895261438434279E-4 4.596840958956186E-5 20-21 0.0 0.0 0.0 9.193681917912372E-4 4.596840958956186E-5 22-23 0.0 0.0 0.0 0.0015629259260451032 4.596840958956186E-5 24-25 0.0 0.0 0.0 0.0021375310459146265 4.596840958956186E-5 26-27 0.0 0.0 0.0 0.0031488360568849875 4.596840958956186E-5 28-29 0.0 0.0 0.0 0.008182376906942011 4.596840958956186E-5 30-31 0.0 0.0 0.0 0.02252452069888531 4.596840958956186E-5 32-33 0.0 4.596840958956186E-5 0.0 0.054541517978015146 4.596840958956186E-5 34-35 0.0 4.596840958956186E-5 0.0 0.10310714270938726 4.596840958956186E-5 36-37 0.0 4.596840958956186E-5 0.0 0.1791388921705226 4.596840958956186E-5 38-39 0.0 4.596840958956186E-5 0.0 0.28608439708063826 4.596840958956186E-5 40-41 0.0 4.596840958956186E-5 0.0 0.40603896190459987 4.596840958956186E-5 42-43 0.0 4.596840958956186E-5 0.0 0.5286596944847561 4.596840958956186E-5 44-45 0.0 4.596840958956186E-5 0.0 0.6818034510323816 4.596840958956186E-5 46-47 0.0 4.596840958956186E-5 0.0 0.8673089679310584 4.596840958956186E-5 48-49 0.0 4.596840958956186E-5 0.0 1.0546072528037282 4.596840958956186E-5 50-51 0.0 4.596840958956186E-5 0.0 1.2641542479177459 4.596840958956186E-5 52-53 0.0 4.596840958956186E-5 0.0 1.4672656656892251 4.596840958956186E-5 54-55 0.0 4.596840958956186E-5 0.0 1.6915225518719026 4.596840958956186E-5 56-57 0.0 4.596840958956186E-5 0.0 1.985858278473867 4.596840958956186E-5 58-59 0.0 4.596840958956186E-5 0.0 2.2829750938560003 4.596840958956186E-5 60-61 0.0 4.596840958956186E-5 0.0 2.6352999691551973 4.596840958956186E-5 62-63 0.0 4.596840958956186E-5 0.0 2.9829590508810533 4.596840958956186E-5 64-65 0.0 4.596840958956186E-5 0.0 3.317287293825937 4.596840958956186E-5 66-67 0.0 4.596840958956186E-5 0.0 3.7165459153160763 4.596840958956186E-5 68-69 0.0 4.596840958956186E-5 0.0 4.176482837464437 4.596840958956186E-5 70-71 0.0 4.596840958956186E-5 0.0 4.673815060813908 4.596840958956186E-5 72-73 0.0 4.596840958956186E-5 0.0 5.169745247670896 4.596840958956186E-5 74-75 0.0 4.596840958956186E-5 0.0 5.694750453593281 4.596840958956186E-5 76-77 0.0 4.596840958956186E-5 0.0 6.216055202543708 6.89526143843428E-5 78-79 0.0 4.596840958956186E-5 0.0 6.8667380402839555 9.193681917912373E-5 80-81 0.0 4.596840958956186E-5 0.0 7.56980188075151 9.193681917912373E-5 82-83 0.0 4.596840958956186E-5 0.0 8.247445190716036 9.193681917912373E-5 84-85 0.0 4.596840958956186E-5 0.0 8.974274698941393 9.193681917912373E-5 86-87 0.0 4.596840958956186E-5 0.0 9.730523989304071 9.193681917912373E-5 88-89 0.0 4.596840958956186E-5 0.0 10.520445139691102 9.193681917912373E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 925 0.0 26.114147 2 TTTGCGT 925 0.0 26.114147 3 GTACATG 10810 0.0 24.981823 1 TACATGG 10705 0.0 24.777002 2 GTAAGCG 280 0.0 23.782076 94-95 TTGCGTT 1030 0.0 23.452024 4 ACATGGG 10790 0.0 23.308832 3 GAGTACT 9270 0.0 22.242367 12-13 CATGGGA 7715 0.0 21.487185 4 ATGGGAT 3105 0.0 20.592999 5 ATGCCGC 140 4.963081E-4 20.289944 6 AGTACTT 9580 0.0 20.21298 12-13 GTACTTT 10290 0.0 20.038736 14-15 CATGGGG 3140 0.0 18.855053 4 ATGGGAG 3140 0.0 18.100851 5 ATTCGCG 175 1.2951205E-9 17.662195 80-81 CGATCGT 165 1.2576493E-8 17.222044 24-25 ACTTTTT 12355 0.0 17.036348 16-17 AGTACAT 8050 0.0 16.886257 2 TCGCGCT 155 1.1011798E-7 16.87799 60-61 >>END_MODULE