##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939007_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2175407 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11926963552108 33.0 31.0 34.0 30.0 34.0 2 32.338327494579175 34.0 31.0 34.0 30.0 34.0 3 32.426278392962786 34.0 31.0 34.0 30.0 34.0 4 35.97592864231843 37.0 35.0 37.0 35.0 37.0 5 35.906367406191116 37.0 35.0 37.0 35.0 37.0 6 35.976560707950284 37.0 35.0 37.0 35.0 37.0 7 35.91352009072325 37.0 35.0 37.0 35.0 37.0 8 35.90039151294447 37.0 35.0 37.0 35.0 37.0 9 37.54653037339679 39.0 37.0 39.0 35.0 39.0 10-11 37.45768768786714 39.0 37.0 39.0 34.0 39.0 12-13 37.37748775286647 39.0 37.0 39.0 34.0 39.0 14-15 38.600067481625274 40.0 38.0 41.0 34.0 41.0 16-17 38.73223470366695 40.0 38.0 41.0 34.0 41.0 18-19 38.81863209964848 40.0 38.0 41.0 34.0 41.0 20-21 38.792393561296805 40.0 38.0 41.0 34.0 41.0 22-23 38.718736080190965 40.0 38.0 41.0 34.0 41.0 24-25 38.6228280501074 40.0 38.0 41.0 34.0 41.0 26-27 38.42580813613269 40.0 38.0 41.0 34.0 41.0 28-29 38.2694293527602 40.0 38.0 41.0 34.0 41.0 30-31 38.08438811679838 40.0 37.5 41.0 33.0 41.0 32-33 37.93991790961415 40.0 37.0 41.0 33.0 41.0 34-35 37.799939045888884 40.0 37.0 41.0 33.0 41.0 36-37 37.63260828893168 40.0 37.0 41.0 33.0 41.0 38-39 37.45884448289446 40.0 37.0 41.0 32.5 41.0 40-41 37.30187707403718 40.0 36.0 41.0 32.0 41.0 42-43 37.08650909921684 40.0 36.0 41.0 31.0 41.0 44-45 36.79097313744049 40.0 36.0 41.0 31.0 41.0 46-47 36.41142508045621 39.5 36.0 41.0 30.0 41.0 48-49 36.00639305656367 39.0 35.0 41.0 29.0 41.0 50-51 35.72203753136769 39.0 35.0 40.5 28.0 41.0 52-53 35.46855967641917 39.0 35.0 40.0 27.5 41.0 54-55 35.198026162460636 38.5 35.0 40.0 27.0 41.0 56-57 35.071027398551166 38.0 34.5 40.0 26.5 41.0 58-59 34.80887461518695 38.0 34.0 40.0 26.0 41.0 60-61 34.4109888402492 38.0 34.0 40.0 25.5 41.0 62-63 34.02128199458768 37.0 33.5 40.0 24.5 41.0 64-65 33.609946552530175 37.0 33.0 39.5 23.0 41.0 66-67 33.14500252136727 36.0 32.5 39.0 22.0 40.0 68-69 32.6450324008335 35.5 32.0 39.0 21.5 40.0 70-71 32.082170141035675 35.0 31.0 38.0 19.5 40.0 72-73 31.549844695728204 35.0 31.0 37.0 18.0 39.0 74-75 31.037608134937507 34.5 30.5 37.0 15.0 39.0 76-77 29.124909959377717 32.5 28.5 35.0 9.0 37.0 78-79 30.07924310255506 34.0 29.5 36.0 8.0 37.5 80-81 30.04544689798277 34.0 30.0 35.0 7.0 37.0 82-83 29.621972348162895 34.0 29.5 35.0 4.0 37.0 84-85 29.15998086794793 34.0 29.0 35.0 2.0 36.0 86-87 28.494535045625945 34.0 28.5 35.0 2.0 36.0 88-89 27.77899239084916 33.0 26.5 35.0 2.0 35.0 90-91 27.167632309724112 33.0 25.5 35.0 2.0 35.0 92-93 26.768272327890827 32.0 25.0 34.0 2.0 35.0 94-95 26.204339923517757 32.0 24.5 34.0 2.0 35.0 96-97 25.321948720400368 32.0 21.5 34.0 2.0 35.0 98-99 24.121862943348074 31.5 9.5 34.0 2.0 35.0 100-101 18.67671428840672 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0963377603950164 1101 2 0.20453773772122474 1101 3 0.1684634162925036 1101 4 0.15028482570864554 1101 5 0.18493786346346042 1101 6 0.13900604845629516 1101 7 0.1360250228506814 1101 8 0.10616373147498592 1101 9 0.14192140929444008 1101 10-11 0.2141781833839218 1101 12-13 0.2020344406965151 1101 14-15 0.2358491912339531 1101 16-17 0.26917976935003196 1101 18-19 0.33012570840252664 1101 20-21 0.3011726093264002 1101 22-23 0.31917373063750176 1101 24-25 0.31835458308922426 1101 26-27 0.2371375813352259 1101 28-29 0.3959151630351556 1101 30-31 0.3178105671067897 1101 32-33 0.3419334340009925 1101 34-35 0.5144794804106567 1101 36-37 0.6136085565424949 1101 38-39 0.6003814801793865 1101 40-41 0.6004374775238333 1101 42-43 0.6241771726514003 1101 44-45 0.686355329033006 1101 46-47 0.7224799725572311 1101 48-49 0.7922524002587608 1101 50-51 0.6830356436604816 1101 52-53 0.5759321040886292 1101 54-55 0.6080078052286311 1101 56-57 0.6222192912023559 1101 58-59 0.677886953516257 1101 60-61 0.6849142859651138 1101 62-63 0.6790328111070849 1101 64-65 0.7682271355197159 1101 66-67 0.5454790540716274 1101 68-69 0.5960370795371475 1101 70-71 0.6708696499789966 1101 72-73 0.7716959483903274 1101 74-75 0.6450955678127634 1101 76-77 0.27468174175193383 1101 78-79 0.6083555559327465 1101 80-81 0.8345032634439278 1101 82-83 0.870435112575418 1101 84-85 0.6909120886097746 1101 86-87 1.0103131097572025 1101 88-89 1.1897531113033928 1101 90-91 1.130510355298071 1101 92-93 1.031169313624595 1101 94-95 0.9037331847612542 1101 96-97 1.1061071456275862 1101 98-99 1.3084514156477702 1101 100-101 1.4763393040728587 1102 1 0.2439029039775562 1102 2 0.20460158556804942 1102 3 0.22719567347663627 1102 4 0.1909724294613966 1102 5 0.180720400201011 1102 6 0.12157683819156517 1102 7 0.1262981163664989 1102 8 0.1581894609053407 1102 9 0.30194339298444106 1102 10-11 0.32454106449922904 1102 12-13 0.27346766210832385 1102 14-15 0.35300373056547585 1102 16-17 0.34995468190351886 1102 18-19 0.30555680656045325 1102 20-21 0.3324812147642291 1102 22-23 0.30782070712897536 1102 24-25 0.35208527537515266 1102 26-27 0.3185226789973967 1102 28-29 0.2566045445122711 1102 30-31 0.20939554608707311 1102 32-33 0.4338479179476451 1102 34-35 0.4775664111319955 1102 36-37 0.4590004946877073 1102 38-39 0.48638365351001056 1102 40-41 0.4979724498689464 1102 42-43 0.44427835270983707 1102 44-45 0.4708374228417682 1102 46-47 0.5365057170106056 1102 48-49 0.5090551504734648 1102 50-51 0.21312312772981556 1102 52-53 0.20177846363463914 1102 54-55 0.29759534813813815 1102 56-57 0.3390768754502176 1102 58-59 0.2732596121155595 1102 60-61 0.27047302973994647 1102 62-63 0.4248624199826807 1102 64-65 0.28400656749922604 1102 66-67 0.2760499038989934 1102 68-69 0.32872805345467526 1102 70-71 0.2791796350310918 1102 72-73 0.2926296542946183 1102 74-75 0.4645893169833677 1102 76-77 0.1475743246352046 1102 78-79 0.49615761258456104 1102 80-81 0.44417608704609535 1102 82-83 0.5002455470662746 1102 84-85 0.6973269193378648 1102 86-87 0.9367583871447991 1102 88-89 0.7414656107087332 1102 90-91 0.6952827439837321 1102 92-93 0.8260930342264139 1102 94-95 0.8974836072837711 1102 96-97 1.225644336372401 1102 98-99 1.4985720254823427 1102 100-101 1.7012567400434833 1103 1 0.19800286786778543 1103 2 0.16052509303073492 1103 3 0.24044896347824363 1103 4 0.08245202743924551 1103 5 0.19568539378149552 1103 6 0.12660462265633754 1103 7 0.1119334477739784 1103 8 0.11324833989747418 1103 9 0.19541604986135042 1103 10-11 0.19457597061382614 1103 12-13 0.27124590797576076 1103 14-15 0.2054112995752604 1103 16-17 0.21428248843538 1103 18-19 0.2837580383049598 1103 20-21 0.32276292115165006 1103 22-23 0.2781818550185591 1103 24-25 0.2588262679927098 1103 26-27 0.23692386861382886 1103 28-29 0.31405469296356614 1103 30-31 0.3028373591989322 1103 32-33 0.352994874696158 1103 34-35 0.38371786154123555 1103 36-37 0.39048194830670724 1103 38-39 0.41352591598793964 1103 40-41 0.4990254008399049 1103 42-43 0.6268132588244271 1103 44-45 0.5628799822222845 1103 46-47 0.7282746947027903 1103 48-49 0.6612079449687371 1103 50-51 0.7870212781069625 1103 52-53 0.7693631711454714 1103 54-55 0.9346379576711215 1103 56-57 0.9390879089921853 1103 58-59 0.8185317393824576 1103 60-61 0.6513431248290189 1103 62-63 0.8908282059720918 1103 64-65 0.8643515662955679 1103 66-67 0.9150148573575123 1103 68-69 0.6975091479820392 1103 70-71 0.7330275827922819 1103 72-73 0.6794749138837766 1103 74-75 0.6259741769576941 1103 76-77 0.7004565867119155 1103 78-79 0.8221431084921207 1103 80-81 0.8257741451427094 1103 82-83 0.705621891152294 1103 84-85 0.7756512753802376 1103 86-87 0.9612255172202282 1103 88-89 0.9264000614389509 1103 90-91 1.0791083237462118 1103 92-93 1.0901434976351787 1103 94-95 1.1108397098355276 1103 96-97 0.9533347652762707 1103 98-99 1.2690946643043617 1103 100-101 1.282577204254686 1104 1 0.04674146279477753 1104 2 0.06086439645165598 1104 3 0.004758281852701884 1104 4 -0.02333802125971829 1104 5 0.043028591520076986 1104 6 0.04829920059867021 1104 7 0.08643496311934484 1104 8 0.06572811863306072 1104 9 0.09069004149696269 1104 10-11 0.10580332039872076 1104 12-13 0.14568565000720213 1104 14-15 0.18671075321129393 1104 16-17 0.1537420169190753 1104 18-19 0.14541305333846566 1104 20-21 0.1668881340596755 1104 22-23 0.17429460930870988 1104 24-25 0.171622689938431 1104 26-27 0.08356299551100221 1104 28-29 0.14733396631747553 1104 30-31 0.07366998450480367 1104 32-33 0.21751152850171707 1104 34-35 0.27293784350902683 1104 36-37 0.2939912572852208 1104 38-39 0.3022726024194071 1104 40-41 0.4874060454014497 1104 42-43 0.2754910300272684 1104 44-45 0.3642189287973636 1104 46-47 0.3566062554685345 1104 48-49 0.34080073588305027 1104 50-51 0.44948334449428273 1104 52-53 0.26300579253302914 1104 54-55 0.49058478864014177 1104 56-57 0.4657129069930477 1104 58-59 0.4503745332954807 1104 60-61 0.4650589745819218 1104 62-63 0.586062739653542 1104 64-65 0.5235029379030323 1104 66-67 0.37736287301473936 1104 68-69 0.5726330944371725 1104 70-71 0.39808165443333365 1104 72-73 0.29160934680874817 1104 74-75 0.2744304188611899 1104 76-77 0.18104122414854373 1104 78-79 0.39861582227976555 1104 80-81 0.43127356939143624 1104 82-83 0.4018651140799925 1104 84-85 0.6107308959841369 1104 86-87 0.7814573020891622 1104 88-89 0.7841628829000804 1104 90-91 0.7010687618369573 1104 92-93 0.9059596900400599 1104 94-95 1.2797899733217015 1104 96-97 0.9710075056524268 1104 98-99 1.2947369618318305 1104 100-101 1.2808708164142324 1105 1 -0.05979370362626213 1105 2 -0.030749098542024456 1105 3 -0.0038775249603801853 1105 4 0.0770094174757574 1105 5 -0.001155131498649098 1105 6 -0.10799065993758461 1105 7 0.009791965104192002 1105 8 -0.019267692328980957 1105 9 0.08685006979148824 1105 10-11 0.04638505376408375 1105 12-13 0.040260839777062074 1105 14-15 -0.07478383820507872 1105 16-17 -0.01720352510162826 1105 18-19 0.027520237811842208 1105 20-21 0.09341503171572896 1105 22-23 0.0474450986451842 1105 24-25 0.02823953591956041 1105 26-27 -0.01953458490358173 1105 28-29 0.04162859135303165 1105 30-31 0.07833148861841721 1105 32-33 0.1305046392407334 1105 34-35 0.21450835501375565 1105 36-37 0.24310264439969842 1105 38-39 0.1314867758210454 1105 40-41 0.2180896492712492 1105 42-43 0.38745992159251585 1105 44-45 0.22533209792300113 1105 46-47 0.32686943004758007 1105 48-49 0.3281953631777057 1105 50-51 0.32216598302706956 1105 52-53 0.3361916500299529 1105 54-55 0.36934244313840026 1105 56-57 0.38752449526393207 1105 58-59 0.2625481035145256 1105 60-61 0.11210355911170211 1105 62-63 0.13780932192569395 1105 64-65 -0.07072223825154111 1105 66-67 -0.0573443692233937 1105 68-69 0.01898146547346613 1105 70-71 0.18469419842187307 1105 72-73 0.21334101664016814 1105 74-75 0.23983496527303316 1105 76-77 0.09253021641588788 1105 78-79 0.19812474897512544 1105 80-81 0.3801839458412921 1105 82-83 0.3064026595928162 1105 84-85 0.42525513714154783 1105 86-87 0.4534861154800929 1105 88-89 0.5282460641495135 1105 90-91 0.41574766582235867 1105 92-93 0.7160855020684878 1105 94-95 0.8252480189344809 1105 96-97 0.8147339202999007 1105 98-99 1.0526985023581297 1105 100-101 1.0837043744827497 1106 1 -0.004156230010295303 1106 2 -0.04283754955481811 1106 3 0.001660327393366856 1106 4 -0.08658276036891976 1106 5 0.02162944811732359 1106 6 0.02520981399695188 1106 7 0.0647843922116067 1106 8 0.03193432971717414 1106 9 -0.08060772972898889 1106 10-11 0.02108921727149493 1106 12-13 -0.030050393998969582 1106 14-15 0.008264735427097492 1106 16-17 -0.20361870591026587 1106 18-19 -0.20587959508152665 1106 20-21 -0.12709353506078003 1106 22-23 -0.03549006789324949 1106 24-25 0.03630273371084769 1106 26-27 0.09908446134042492 1106 28-29 -0.057014622012516725 1106 30-31 -0.03862149797021175 1106 32-33 -0.11809034700137744 1106 34-35 -0.145781440862784 1106 36-37 -0.1486802082614318 1106 38-39 -0.02170210590583821 1106 40-41 0.060685027664383995 1106 42-43 0.19928658152772272 1106 44-45 0.1728232163078971 1106 46-47 0.2396508752498221 1106 48-49 0.16449373700847048 1106 50-51 0.13194607572747685 1106 52-53 0.20714776730639528 1106 54-55 0.28370425228660423 1106 56-57 0.3781479175779836 1106 58-59 0.24274866873361844 1106 60-61 0.3605807137533361 1106 62-63 0.3875021680106059 1106 64-65 0.3420275367972252 1106 66-67 0.3143943035736285 1106 68-69 0.09437226585276193 1106 70-71 0.31795510180368325 1106 72-73 0.34403186377951656 1106 74-75 0.319305044429786 1106 76-77 -0.015125484304888914 1106 78-79 0.1603870831026981 1106 80-81 0.19497106652134732 1106 82-83 0.2343076509953015 1106 84-85 0.3787546192413913 1106 86-87 0.590019174462963 1106 88-89 0.7504496827439908 1106 90-91 0.6605881087946592 1106 92-93 0.6962422879577588 1106 94-95 0.9795057683415997 1106 96-97 0.9832058633383376 1106 98-99 1.276972324368142 1106 100-101 1.3370979711503068 1107 1 0.04954920079535441 1107 2 0.0397114137854544 1107 3 0.02664418563283988 1107 4 0.03820560321526756 1107 5 0.08572837917407128 1107 6 -0.09756800363702922 1107 7 -0.030469930193156358 1107 8 0.03565590228335225 1107 9 -0.03995560260365494 1107 10-11 0.0061553984896534075 1107 12-13 0.08246395423261532 1107 14-15 -0.024416949055293458 1107 16-17 0.024031233109752748 1107 18-19 -0.05994821549905538 1107 20-21 0.10123884172377018 1107 22-23 0.07642344814089341 1107 24-25 0.03704256440171605 1107 26-27 0.08594148121272838 1107 28-29 0.07982804962819756 1107 30-31 0.08510778677045039 1107 32-33 0.12781323288240287 1107 34-35 0.1791539920951024 1107 36-37 0.2403954069189993 1107 38-39 0.19856566796602948 1107 40-41 0.2737562666341802 1107 42-43 0.3393360829266783 1107 44-45 0.22946644752965284 1107 46-47 0.13119617979104703 1107 48-49 0.20443203961266931 1107 50-51 0.18588197500288572 1107 52-53 0.24723044899087654 1107 54-55 0.16821982011786218 1107 56-57 0.3618016947499427 1107 58-59 0.3256509572765509 1107 60-61 0.3846767751637685 1107 62-63 0.5542097869215752 1107 64-65 0.445499808839692 1107 66-67 0.4331124684348069 1107 68-69 0.42085820458581225 1107 70-71 0.4586466865658636 1107 72-73 0.511169032873422 1107 74-75 0.539922556446431 1107 76-77 0.3117792434374209 1107 78-79 0.6663667086138503 1107 80-81 0.6362123679059479 1107 82-83 0.5124310816574713 1107 84-85 0.7874846213936273 1107 86-87 0.9332441188635627 1107 88-89 0.9240292078175258 1107 90-91 1.0092142656208303 1107 92-93 1.0941196464483234 1107 94-95 1.2177877469627347 1107 96-97 1.3395746846311098 1107 98-99 1.1571376877371087 1107 100-101 1.1652375435730065 1108 1 0.05069677525080607 1108 2 0.0492286714862189 1108 3 0.05784053084254026 1108 4 0.06694206480655396 1108 5 -0.040322442643301315 1108 6 0.1060176444896399 1108 7 0.09072144977324825 1108 8 0.07967255208985335 1108 9 0.07319950788460972 1108 10-11 0.03373488878655451 1108 12-13 0.1558768165329596 1108 14-15 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-1.2686965749369747 2316 60-61 -1.3224533444864832 2316 62-63 -1.2651636311640218 2316 64-65 -1.3295656248924743 2316 66-67 -1.3889473279296674 2316 68-69 -1.2317972544569855 2316 70-71 -1.0642285147686934 2316 72-73 -0.9729456703019075 2316 74-75 -1.2177389426331793 2316 76-77 -1.0127490584493728 2316 78-79 -1.1747896708155068 2316 80-81 -1.2567150366039428 2316 82-83 -1.3792768106573021 2316 84-85 -1.5697030224167747 2316 86-87 -1.6178891070913402 2316 88-89 -1.8472525742809545 2316 90-91 -1.7838317824693668 2316 92-93 -1.81328515106663 2316 94-95 -1.788261637528752 2316 96-97 -1.6517744007347446 2316 98-99 -1.9662084926586623 2316 100-101 -1.5165352072323337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 24.0 5 64.0 6 157.0 7 366.0 8 748.0 9 1490.0 10 2612.0 11 3909.0 12 6065.0 13 8252.0 14 9874.0 15 13241.0 16 17247.0 17 24284.0 18 23426.0 19 15104.0 20 13757.0 21 14417.0 22 15686.0 23 17346.0 24 19905.0 25 23263.0 26 27457.0 27 33075.0 28 40122.0 29 48320.0 30 58386.0 31 71145.0 32 87880.0 33 110951.0 34 149283.0 35 211916.0 36 323551.0 37 466704.0 38 300103.0 39 15268.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.342166408471925 19.74467562757012 14.536802326198483 21.37635563775947 2 15.178079320329482 22.694052193451615 38.68779497353829 23.440073512680616 3 21.307231244544127 25.772786425712525 30.80899344352574 22.110988886217616 4 12.54482494540102 16.924557105865706 38.77265265764061 31.757965291092656 5 12.289884145817313 39.40099484832034 34.43061459303937 13.878506412822981 6 29.54201213841824 37.29030935360601 17.316805544893437 15.85087296308231 7 26.168528463869063 34.822633190019154 21.394157507077985 17.6146808390338 8 25.040141913674084 33.398531860934526 20.286272867559955 21.275053357831432 9 25.20925969255408 18.380008890290416 22.231655961390214 34.17907545576529 10-11 24.600890775841027 26.40053102706758 27.499681668763593 21.4988965283278 12-13 27.411721117013965 24.430554834106903 27.33573533596242 20.82198871291671 14-15 22.94650952991134 25.884284983709797 28.48198309496584 22.687222391413023 16-17 20.474870819608526 29.02850783287927 28.824678517176206 21.671942830336 18-19 20.202808865320275 28.93852067529712 30.481502695139007 20.3771677642436 20-21 22.181136679251285 26.519313397447007 29.62025037153967 21.67929955176204 22-23 20.991244397025476 26.995913868071586 29.873697197811723 22.13914453709122 24-25 22.11314674738727 26.78508139863612 30.165900142732045 20.935871711244573 26-27 21.97860491820724 25.807452538180797 31.603633619739576 20.610308923872388 28-29 20.42836122160129 28.404891590401242 30.604985641767264 20.5617615462302 30-31 21.05904780117008 27.04951303365301 30.1278795186372 21.763559646539704 32-33 20.96550208765532 27.269977526044553 30.42630643369264 21.33821395260749 34-35 22.19417257460135 26.58275219454267 30.16647652315755 21.05659870769843 36-37 21.824393215741043 26.17806668160997 31.228764706220435 20.768775396428552 38-39 21.40436960758307 26.99312832475389 30.987315421709017 20.615186645954022 40-41 21.263769794888237 27.486768929481407 30.3157872961036 20.933673979526752 42-43 20.572893202383863 27.958499364682993 29.29799769410268 22.170609738830464 44-45 21.46515798810271 26.905911846240375 29.244043368264567 22.38488679739235 46-47 22.26027428745848 27.794106966040903 28.778830568608182 21.166788177892435 48-49 21.974141065699584 27.38453419486876 29.020852362916894 21.620472376514762 50-51 20.79281766558962 28.078098991768385 29.933533848826393 21.195549493815598 52-53 21.592718052300096 28.867195885643472 28.098948840377915 21.44113722167852 54-55 20.576356516274885 27.479363631725008 29.031578918335743 22.912700933664368 56-57 21.902522148728952 27.507473314189024 28.283718862723163 22.306285674358868 58-59 22.854706512395477 27.243758590413673 28.068507256170154 21.833027641020692 60-61 21.51658414834588 27.483691895950834 29.73849802022685 21.26122593547643 62-63 21.440019343742396 27.490179304264245 29.320939486218617 21.748861865774746 64-65 20.92672450449183 28.761010079558037 28.633079172361043 21.679186243589086 66-67 21.294873577669772 27.48618324198777 28.11566887589224 23.10327430445021 68-69 20.777976315524544 27.975258222933423 28.55155655483327 22.695208906708764 70-71 21.92587410080045 27.62639358979722 27.86986527118833 22.577867038214 72-73 21.503470385081965 26.72954991870487 29.06426245755392 22.702717238659247 74-75 20.680520932404832 29.00415876201557 27.83430870637081 22.48101159920879 76-77 21.2219554922663 27.230142964444653 28.040907454418505 23.506994088870535 78-79 21.146381333975686 27.289029904400802 28.706652593719028 22.857936167904487 80-81 21.677541660662033 27.527261034562095 28.113566057974992 22.681631246800883 82-83 21.2566889782004 27.13257794978135 28.83869547169794 22.772037600320306 84-85 20.84661056061311 27.42221674686633 28.435844560366718 23.295328132153845 86-87 20.97738467629671 27.66123969141953 28.11272716146319 23.248648470820566 88-89 20.981949628491485 27.247905265386148 27.662800878960965 24.10734422716141 90-91 21.042472066958542 26.364637952019198 28.22503331199059 24.367856669031674 92-93 21.500482438458643 27.509312050572603 28.025054621962695 22.965150889006058 94-95 21.0669085239047 27.024075705115592 27.976434548687408 23.9325812222923 96-97 19.75002838549292 26.96699973843975 28.933137569199697 24.349834306867635 98-99 20.73522333981641 27.323254912758856 27.391288158951404 24.55023358847333 100-101 20.735396296356736 25.97583844982885 28.52978431308103 24.758980940733387 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1630.0 1 1148.5 2 729.5 3 829.0 4 766.0 5 782.5 6 1232.0 7 2217.5 8 3319.5 9 3761.5 10 3386.0 11 3027.0 12 3134.0 13 3348.0 14 3595.5 15 3811.0 16 4003.5 17 4320.0 18 4694.0 19 5124.0 20 5707.0 21 6426.0 22 7533.0 23 9040.0 24 10525.5 25 12731.5 26 16012.5 27 19209.5 28 21717.5 29 24348.5 30 27480.0 31 31179.0 32 34990.0 33 38578.0 34 42425.0 35 45850.5 36 50307.0 37 55039.0 38 56897.5 39 57034.5 40 58106.5 41 59931.5 42 61890.0 43 64329.5 44 67642.0 45 71010.0 46 76313.0 47 101360.0 48 155308.0 49 149995.0 50 95434.0 51 75256.5 52 69078.5 53 63553.5 54 57799.0 55 52349.0 56 46656.5 57 40588.0 58 36119.0 59 32194.5 60 27109.5 61 22078.5 62 17688.0 63 13255.5 64 9357.0 65 7025.5 66 4964.0 67 3598.5 68 2605.0 69 1633.0 70 1151.5 71 856.0 72 582.5 73 468.5 74 368.0 75 292.0 76 205.0 77 90.0 78 35.0 79 17.0 80 8.5 81 8.5 82 9.0 83 5.0 84 2.0 85 1.0 86 1.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.387881440116723 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.963839869964562E-4 16-17 0.0026431835513998072 18-19 4.137156863060568E-4 20-21 0.0 22-23 0.0 24-25 9.193681917912373E-5 26-27 2.298420479478093E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013583665033715529 36-37 0.023880588781777387 38-39 0.011308228759032217 40-41 0.005952909041848261 42-43 0.0052863671027996145 44-45 0.00172381535960857 46-47 0.0011492102397390467 48-49 0.003033915032911083 50-51 6.43557734253866E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.814629630225517E-4 60-61 0.0014480049020711987 62-63 0.0012181628541233893 64-65 6.43557734253866E-4 66-67 0.0027351203705789306 68-69 0.002666167756194588 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09030494063869426 78-79 0.00344763071921714 80-81 1.608894335634665E-4 82-83 0.0 84-85 1.379052287686856E-4 86-87 0.09356869771955316 88-89 0.43396477073025874 90-91 0.3858818142995771 92-93 0.0 94-95 8.733997822016754E-4 96-97 0.0 98-99 0.0 100-101 3.9073148151127585E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2175407.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.94931565778082 #Duplication Level Percentage of deduplicated Percentage of total 1 71.78542852964698 34.42062172197108 2 14.938498172076889 14.325815286121932 3 5.581659505794788 8.029102606128216 4 2.7175700185198974 5.212224905605273 5 1.4502339229442072 3.4768862074436786 6 0.8869653258706983 2.5517628232608325 7 0.5965863856074355 2.002413624443783 8 0.40468749632920203 1.552359080339674 9 0.2817892725234776 1.2160442499484185 >10 1.2469676313492346 10.267861603146267 >50 0.06647400444807765 2.240422387127774 >100 0.03584004073901764 3.013328416415894 >500 0.002497263788206718 0.848925361049948 >1k 0.003265652646116469 3.650659985878975 >5k 0.0010565346796259163 3.658811658905929 >10k+ 4.8024303619359845E-4 3.5327600822122975 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 29939 1.3762482147018926 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14057 0.6461779336004712 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11351 0.5217874172511167 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11180 0.5139268192113017 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10181 0.4680043780313293 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9467 0.43518293358438215 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8362 0.3843878409879163 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8143 0.37432075928780223 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7478 0.3437517669107436 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7197 0.3308346438160767 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7176 0.32986930721469593 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7024 0.32288210895708247 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6677 0.30693107082950455 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6312 0.2901526013293145 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5920 0.27213298477020625 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5689 0.26151428215501743 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4948 0.2274516906491521 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4259 0.19577945644194397 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3908 0.17964454467600777 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3881 0.1784033976170896 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3592 0.1651185272457062 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3539 0.1626822015374594 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3409 0.1567063082908164 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3276 0.15059250981540465 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3206 0.14737472114413533 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2863 0.1316075566549156 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2824 0.1298147886809227 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2758 0.1267808736480116 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2641 0.12140256972603287 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2619 0.12039126471506251 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2517 0.11570248693692721 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2183 0.10034903813401354 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.596840958956186E-5 0.0 5 0.0 0.0 0.0 4.596840958956186E-5 0.0 6 0.0 0.0 0.0 4.596840958956186E-5 0.0 7 0.0 0.0 0.0 4.596840958956186E-5 0.0 8 0.0 0.0 0.0 4.596840958956186E-5 0.0 9 0.0 0.0 0.0 4.596840958956186E-5 0.0 10-11 0.0 0.0 0.0 1.1492102397390466E-4 0.0 12-13 0.0 0.0 0.0 2.758104575373712E-4 0.0 14-15 0.0 0.0 0.0 3.4476307192171397E-4 0.0 16-17 0.0 0.0 0.0 4.8266830069039956E-4 0.0 18-19 0.0 0.0 0.0 6.43557734253866E-4 0.0 20-21 0.0 0.0 0.0 8.733997822016754E-4 0.0 22-23 0.0 0.0 0.0 0.0015169575164555413 0.0 24-25 0.0 0.0 0.0 0.0021605152507094072 0.0 26-27 0.0 0.0 0.0 0.0031948044664745494 0.0 28-29 0.0 0.0 0.0 0.008251329521326355 0.0 30-31 0.0 0.0 0.0 0.022846299566012244 0.0 32-33 0.0 0.0 0.0 0.0552080599170638 0.0 34-35 0.0 0.0 0.0 0.10480797386420104 0.0 36-37 0.0 0.0 0.0 0.182034901974665 0.0 38-39 0.0 0.0 0.0 0.2902215539436988 0.0 40-41 0.0 0.0 0.0 0.4113942816217839 0.0 42-43 0.0 0.0 0.0 0.5344976825026305 0.0 44-45 0.0 0.0 0.0 0.6883769336036889 0.0 46-47 0.0 0.0 0.0 0.8748707713085413 0.0 48-49 0.0 0.0 0.0 1.0634102032401294 0.0 50-51 0.0 0.0 0.0 1.273370914040453 0.0 52-53 0.0 0.0 0.0 1.4781142103523615 0.0 54-55 0.0 0.0 0.0 1.7043937065569799 0.0 56-57 0.0 0.0 0.0 2.000453248518553 0.0 58-59 0.0 0.0 0.0 2.3004660737048286 0.0 60-61 0.0 0.0 0.0 2.6550434010739137 0.0 62-63 0.0 0.0 0.0 3.0044262981593786 0.0 64-65 0.0 0.0 0.0 3.341834424546763 0.0 66-67 0.0 0.0 0.0 3.744149945274608 0.0 68-69 0.0 0.0 0.0 4.206224398468884 0.0 70-71 0.0 0.0 0.0 4.70767079447662 0.0 72-73 0.0 0.0 0.0 5.2088873484364075 0.0 74-75 0.0 0.0 0.0 5.7393398108951565 0.0 76-77 0.0 0.0 0.0 6.266528516273047 0.0 78-79 0.0 0.0 0.0 6.918820248348929 0.0 80-81 0.0 0.0 0.0 7.623240156899376 0.0 82-83 0.0 0.0 0.0 8.303112934728995 0.0 84-85 0.0 0.0 0.0 9.03453928391331 0.0 86-87 0.0 0.0 0.0 9.792788200093131 0.0 88-89 0.0 0.0 0.0 10.583973481743875 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 715 0.0 29.20495 3 GTAAGCG 450 0.0 28.47548 94-95 CTTTGCG 795 0.0 26.86304 2 TTGCGTT 790 0.0 26.432327 4 GTACATG 10215 0.0 23.08537 1 TACATGG 10295 0.0 22.910778 2 GAGTACT 10095 0.0 22.591072 12-13 ACATGGG 10355 0.0 21.769745 3 GTACTTT 10925 0.0 20.809605 14-15 AGTACTT 10570 0.0 20.251225 12-13 CATGGGA 7170 0.0 19.592163 4 ACCGTAG 125 0.005931285 19.055923 1 CTCCGAC 550 0.0 18.983654 94-95 GTATAGC 830 0.0 18.941128 1 GTGTAGC 1225 0.0 17.889235 1 ACTTTTT 13210 0.0 17.298494 16-17 AGTACAT 8025 0.0 17.209084 2 ATGGGAT 2775 0.0 17.101997 5 TACTTTT 12515 0.0 16.990137 14-15 GTAGCCC 1065 0.0 16.933386 3 >>END_MODULE