##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939006_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2181771 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.865555092628878 33.0 31.0 34.0 30.0 34.0 2 31.89979470806056 33.0 31.0 34.0 30.0 34.0 3 31.953660122900157 34.0 31.0 34.0 30.0 34.0 4 35.47960349642561 37.0 35.0 37.0 33.0 37.0 5 35.53591188076109 37.0 35.0 37.0 33.0 37.0 6 35.63976008481183 37.0 35.0 37.0 33.0 37.0 7 35.56280104557261 37.0 35.0 37.0 33.0 37.0 8 35.559177383877596 37.0 35.0 37.0 33.0 37.0 9 37.15912256602549 39.0 37.0 39.0 33.0 39.0 10-11 37.053056897355404 39.0 37.0 39.0 33.0 39.0 12-13 36.94641486205472 39.0 37.0 39.0 33.0 39.0 14-15 38.100538278306935 40.0 38.0 41.0 33.0 41.0 16-17 38.06084735749077 40.0 38.0 41.0 33.0 41.0 18-19 38.064434810069436 40.0 38.0 41.0 33.0 41.0 20-21 37.95180452027275 40.0 38.0 41.0 33.0 41.0 22-23 38.022562175407046 40.0 38.0 41.0 33.0 41.0 24-25 37.944520529423116 40.0 38.0 41.0 33.0 41.0 26-27 37.83388036599625 40.0 37.5 41.0 32.5 41.0 28-29 37.721290410405125 40.0 37.0 41.0 32.0 41.0 30-31 37.55402102237127 40.0 37.0 41.0 32.0 41.0 32-33 37.3359605568137 40.0 36.5 41.0 31.5 41.0 34-35 36.98632922520282 40.0 36.0 41.0 30.5 41.0 36-37 36.68884360457628 39.5 35.5 41.0 30.0 41.0 38-39 36.46542487731297 39.0 35.0 41.0 30.0 41.0 40-41 36.33934267161861 39.0 35.0 41.0 30.0 41.0 42-43 36.123165996798015 39.0 35.0 41.0 30.0 41.0 44-45 35.990193287929856 39.0 35.0 41.0 29.0 41.0 46-47 35.69710019979182 39.0 35.0 41.0 27.0 41.0 48-49 35.45566697879842 39.0 35.0 41.0 26.0 41.0 50-51 34.242638663727774 38.0 34.0 40.0 23.5 40.5 52-53 34.40126003141485 38.0 34.5 39.5 24.0 40.5 54-55 34.92530494721949 39.0 35.0 40.5 24.0 41.0 56-57 34.82158072501651 39.0 35.0 41.0 23.5 41.0 58-59 34.64669069301957 38.5 34.5 40.5 23.0 41.0 60-61 34.33497374380721 38.0 34.0 40.0 23.0 41.0 62-63 34.0111026775954 38.0 34.0 40.0 22.0 41.0 64-65 33.603032811417876 37.0 33.5 40.0 21.5 41.0 66-67 33.191929400473285 37.0 33.0 39.5 20.0 41.0 68-69 32.788859600755536 36.0 33.0 39.0 18.5 41.0 70-71 32.330108659433094 35.5 32.0 39.0 17.0 40.0 72-73 31.875079923603344 35.0 31.5 38.0 13.0 40.0 74-75 31.423270361554902 35.0 31.0 37.0 9.0 39.5 76-77 31.007082319821833 35.0 31.0 36.5 7.0 39.0 78-79 30.560835898909648 35.0 31.0 36.0 4.0 39.0 80-81 30.099469880202825 34.0 30.0 36.0 2.0 37.5 82-83 29.713975939729696 34.0 30.0 35.0 2.0 37.0 84-85 29.34500871081337 34.0 29.5 35.0 2.0 37.0 86-87 28.975589784629094 34.0 29.0 35.0 2.0 36.0 88-89 28.531156340422527 34.0 29.0 35.0 2.0 36.0 90-91 28.064732962350313 34.0 28.5 35.0 2.0 35.5 92-93 27.647702714904543 34.0 27.0 35.0 2.0 35.0 94-95 27.159651264958605 34.0 26.0 35.0 2.0 35.0 96-97 26.55023808639862 33.0 25.0 35.0 2.0 35.0 98-99 25.875249968947244 33.0 24.0 35.0 2.0 35.0 100-101 23.320743102736266 30.0 9.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24018173782077312 1101 2 0.27715230542534997 1101 3 0.2693643167451185 1101 4 0.149395900213257 1101 5 -0.018486825862844114 1101 6 0.08891545123159972 1101 7 0.15590637359304793 1101 8 0.24329080579931173 1101 9 0.2618235943999494 1101 10-11 0.2971004254771472 1101 12-13 0.34460894405197706 1101 14-15 0.07010937685457463 1101 16-17 0.43532180135171217 1101 18-19 0.3254846583049513 1101 20-21 0.46560097281153645 1101 22-23 0.3661135923529457 1101 24-25 0.5010667318873345 1101 26-27 0.5046077509143672 1101 28-29 0.40792431279267305 1101 30-31 0.47840979735548217 1101 32-33 0.41913659469035736 1101 34-35 0.17625388140113074 1101 36-37 0.20347540477955306 1101 38-39 0.28337171727129373 1101 40-41 0.2777569368430832 1101 42-43 0.16465755225751622 1101 44-45 0.27978715918271035 1101 46-47 0.4546886428321457 1101 48-49 0.4389613795870275 1101 50-51 0.3542463591728193 1101 52-53 0.3112084973511102 1101 54-55 0.34472491388286386 1101 56-57 0.2691918167515084 1101 58-59 0.27835484123729515 1101 60-61 0.16512060605710133 1101 62-63 0.17068005627839966 1101 64-65 0.32418290864602284 1101 66-67 0.3077218469332266 1101 68-69 0.34786736658701045 1101 70-71 0.28469946999284446 1101 72-73 0.20610454088693686 1101 74-75 0.1777256919169865 1101 76-77 0.059723689849970896 1101 78-79 -0.01850558324504803 1101 80-81 -0.16138078066519768 1101 82-83 -0.24463797413027422 1101 84-85 -0.2628122380647717 1101 86-87 -0.4219534543047416 1101 88-89 -0.4315854068868994 1101 90-91 -0.4976762061775304 1101 92-93 -0.44250687236031183 1101 94-95 -0.5774493287754474 1101 96-97 -0.7207316496209408 1101 98-99 -0.8272252661771802 1101 100-101 -0.8049654132678405 1102 1 0.17575715502465172 1102 2 0.08498940105991792 1102 3 0.07468617014284007 1102 4 0.21702991170776187 1102 5 0.25974791551081466 1102 6 0.26410028839278965 1102 7 0.3044003604668788 1102 8 0.12917616669614773 1102 9 0.3148986291821174 1102 10-11 0.20755219299388017 1102 12-13 0.20013147779613405 1102 14-15 0.38852717075476306 1102 16-17 0.2944365497182275 1102 18-19 0.3751550472096241 1102 20-21 0.4694692594894221 1102 22-23 0.4970383973178656 1102 24-25 0.4154884009942137 1102 26-27 0.45229537429089817 1102 28-29 0.3831619605713499 1102 30-31 0.46321745744449316 1102 32-33 0.4129857967037154 1102 34-35 0.31934807369005114 1102 36-37 0.3364164826988798 1102 38-39 0.4911352422525397 1102 40-41 0.39708562284207716 1102 42-43 0.2527019568815376 1102 44-45 0.49772200909129083 1102 46-47 0.4300858150967386 1102 48-49 0.3823170754852825 1102 50-51 0.4122855919984403 1102 52-53 0.36232662559714157 1102 54-55 0.2818810074996989 1102 56-57 0.3204549010372588 1102 58-59 0.3214894609644219 1102 60-61 0.17591983101576147 1102 62-63 0.1412062685552371 1102 64-65 0.2479363959678338 1102 66-67 0.27069978302144904 1102 68-69 0.09318251849948922 1102 70-71 0.04196657516973801 1102 72-73 0.0712786702401722 1102 74-75 0.09875706506588244 1102 76-77 -0.008037067126497988 1102 78-79 0.01217337919021233 1102 80-81 -0.05957210806917246 1102 82-83 -0.18517249042143646 1102 84-85 -0.18027689137216996 1102 86-87 -0.15619877237034174 1102 88-89 -0.12243460161603537 1102 90-91 -0.22398718423016462 1102 92-93 -0.24832623819584754 1102 94-95 -0.19132504536089456 1102 96-97 -0.5572791463976721 1102 98-99 -0.6414546317941898 1102 100-101 -0.3332613421155486 1103 1 -0.027940250662013 1103 2 0.21102673250256387 1103 3 0.22823139865838726 1103 4 0.1551349216415474 1103 5 0.054452121134445974 1103 6 0.0493558672616885 1103 7 0.1321810043761431 1103 8 0.028748360545264973 1103 9 0.2284917300994067 1103 10-11 0.2788077843520611 1103 12-13 0.24368835017098434 1103 14-15 0.20960358886538444 1103 16-17 0.23420851933659748 1103 18-19 0.24283956683317598 1103 20-21 0.19828034171504783 1103 22-23 0.3700799808827355 1103 24-25 0.3924873355618743 1103 26-27 0.24364044179069566 1103 28-29 0.21697012702858132 1103 30-31 0.31823111400060355 1103 32-33 0.2918997474305982 1103 34-35 0.285062936519239 1103 36-37 0.20364850580983074 1103 38-39 0.387274202874778 1103 40-41 0.39715551481933886 1103 42-43 0.2938957118532386 1103 44-45 0.484853118754927 1103 46-47 0.4764677884426689 1103 48-49 0.3771060401425217 1103 50-51 0.20822743803049093 1103 52-53 0.3141272206636856 1103 54-55 0.4086018872835737 1103 56-57 0.4079314355864696 1103 58-59 0.3551255154254065 1103 60-61 0.34176616761936884 1103 62-63 0.24758269857871085 1103 64-65 0.2174983197793381 1103 66-67 0.11910660620771552 1103 68-69 0.22613327131533367 1103 70-71 0.2894703988133003 1103 72-73 0.13943811905906855 1103 74-75 0.2065182449000993 1103 76-77 0.05619654212403091 1103 78-79 0.28071264647250516 1103 80-81 0.07440853065223152 1103 82-83 0.1308110602255077 1103 84-85 0.11220073839153244 1103 86-87 0.2408543080645167 1103 88-89 0.19879517054092233 1103 90-91 0.23937524914428465 1103 92-93 0.26545136793427915 1103 94-95 0.3811011765718355 1103 96-97 0.130641406359004 1103 98-99 0.09922295531378822 1103 100-101 0.2015896558746384 1104 1 0.22024560050155984 1104 2 0.16431764041545804 1104 3 0.1990792464528255 1104 4 0.2324077528571067 1104 5 0.2244918913930789 1104 6 0.19360690201254727 1104 7 0.17998106306919226 1104 8 0.12533749394618354 1104 9 0.3146947661308417 1104 10-11 0.26791455425243527 1104 12-13 0.33105714833549627 1104 14-15 0.40816546620452954 1104 16-17 0.1992687654662788 1104 18-19 0.34483803662150336 1104 20-21 0.3388089888783483 1104 22-23 0.32108462296946527 1104 24-25 0.33936941017064726 1104 26-27 0.3615339139038767 1104 28-29 0.27048132444303974 1104 30-31 0.3780830188568771 1104 32-33 0.39606752570806947 1104 34-35 0.42422096755795025 1104 36-37 0.2946858578385516 1104 38-39 0.29803051428333305 1104 40-41 0.32949509985185443 1104 42-43 0.21751042246600605 1104 44-45 0.3436317792575565 1104 46-47 0.3876133224350937 1104 48-49 0.39034651586112545 1104 50-51 0.37847444430379085 1104 52-53 0.20160116133034478 1104 54-55 0.17709067462417494 1104 56-57 0.310652230076947 1104 58-59 0.3455221508653352 1104 60-61 0.19503901979673088 1104 62-63 0.20211773718654058 1104 64-65 0.148154290844424 1104 66-67 0.22860749613156628 1104 68-69 0.22984470159637738 1104 70-71 0.3796104427187572 1104 72-73 0.2358478861162574 1104 74-75 0.21058150000144238 1104 76-77 0.15192290673745745 1104 78-79 0.13648519375089663 1104 80-81 0.053811891019940106 1104 82-83 -0.023787483119690478 1104 84-85 0.09280416202577513 1104 86-87 0.060252408925091316 1104 88-89 0.13519419943772348 1104 90-91 0.144090035219552 1104 92-93 0.17035909788526737 1104 94-95 0.2091250596888159 1104 96-97 0.23006133607979606 1104 98-99 0.3384307229960264 1104 100-101 0.5903508418553791 1105 1 0.02316766613914112 1105 2 -0.0591053955834262 1105 3 0.03517539481520515 1105 4 0.08774040267710603 1105 5 0.05818036250418146 1105 6 0.08389147672851749 1105 7 0.18029159714165388 1105 8 0.1423370426797348 1105 9 0.07835295901633543 1105 10-11 0.07775696167129098 1105 12-13 0.175265480246118 1105 14-15 0.10123852615098627 1105 16-17 0.15426901001763582 1105 18-19 0.24696258800819493 1105 20-21 0.32890788913554303 1105 22-23 0.23848671245310982 1105 24-25 0.19671337948822298 1105 26-27 0.22069817581559903 1105 28-29 0.3027881727113879 1105 30-31 0.17267465040198005 1105 32-33 0.2505564733713115 1105 34-35 0.255567513431636 1105 36-37 0.25181046920954486 1105 38-39 0.16088334881804656 1105 40-41 0.1497643924155554 1105 42-43 0.1253094880030048 1105 44-45 0.1731504191460047 1105 46-47 0.20446028035473063 1105 48-49 0.03940933049028672 1105 50-51 -0.043676324833270996 1105 52-53 0.05249704982231407 1105 54-55 0.11936363638095315 1105 56-57 0.1489228090730279 1105 58-59 0.08340347793938463 1105 60-61 0.36329750042226294 1105 62-63 0.12304644170114898 1105 64-65 0.14323327170205147 1105 66-67 0.033143831386546196 1105 68-69 0.10313203466701282 1105 70-71 0.0024202085308431265 1105 72-73 0.06889544812387172 1105 74-75 0.184172722730505 1105 76-77 0.020320135647672544 1105 78-79 -0.08735888584548945 1105 80-81 0.08957037395256151 1105 82-83 0.03570032484197938 1105 84-85 0.06779094241035466 1105 86-87 -6.944016177463652E-4 1105 88-89 0.08234849841974423 1105 90-91 -0.009232817781093416 1105 92-93 0.18703773064942908 1105 94-95 0.23289314379971415 1105 96-97 0.179154050572091 1105 98-99 0.32681245750185184 1105 100-101 0.6970744351544909 1106 1 0.04198979964939298 1106 2 -0.0016401928906688568 1106 3 -0.007169876894760563 1106 4 -0.05863993013618085 1106 5 -0.06879703254912783 1106 6 -0.11951862541604186 1106 7 0.07514746032092745 1106 8 -0.027736480571682876 1106 9 -0.06977612670946343 1106 10-11 0.021431856209467526 1106 12-13 -0.004575064645628402 1106 14-15 -0.022592789274405334 1106 16-17 0.15130305895916507 1106 18-19 -0.049926882565522135 1106 20-21 0.13684747190750812 1106 22-23 0.008030492413212187 1106 24-25 0.13911107076302898 1106 26-27 0.13202884024207862 1106 28-29 0.10474366023598236 1106 30-31 0.0497142016995582 1106 32-33 0.02637475070364559 1106 34-35 -0.029209301496734952 1106 36-37 -0.18971363812941888 1106 38-39 -0.11449406072706836 1106 40-41 0.03946502348824055 1106 42-43 0.11791029093939898 1106 44-45 0.031783117632741664 1106 46-47 -0.15219440038408294 1106 48-49 0.0019055660182942802 1106 50-51 -0.05621558289830375 1106 52-53 -0.04043412091149179 1106 54-55 -0.14392093052928345 1106 56-57 -0.21076292261579255 1106 58-59 0.02023156214580979 1106 60-61 0.16010352991760612 1106 62-63 0.10704986991804333 1106 64-65 -0.09904950411203828 1106 66-67 -0.09028692157577467 1106 68-69 0.021889181502928068 1106 70-71 0.09074525059104133 1106 72-73 0.020447316320929332 1106 74-75 0.0798983204607282 1106 76-77 6.461559044943499E-4 1106 78-79 0.06300137129342076 1106 80-81 0.12540973232803054 1106 82-83 0.1416432090275741 1106 84-85 0.1561038702298596 1106 86-87 0.15964685767455578 1106 88-89 0.17597595494999396 1106 90-91 0.17628002218186012 1106 92-93 0.0038385974403283285 1106 94-95 0.005320905563547029 1106 96-97 -0.09519602607595701 1106 98-99 -0.180484504427735 1106 100-101 0.2892127458179665 1107 1 0.041753923297100926 1107 2 -0.03550828951179241 1107 3 0.004960119633228288 1107 4 0.042434189047504844 1107 5 0.09023984241986938 1107 6 0.11676114157339157 1107 7 -0.01710444239660802 1107 8 0.03665115024875831 1107 9 -0.03893364030817992 1107 10-11 0.055637785082829794 1107 12-13 0.016255352381556065 1107 14-15 -0.005516553816896419 1107 16-17 0.10243807612633304 1107 18-19 0.129807591204262 1107 20-21 0.11469713931082737 1107 22-23 0.04095343028625109 1107 24-25 0.2437194774403082 1107 26-27 0.26348967539867374 1107 28-29 0.20273044454689426 1107 30-31 0.292435377113307 1107 32-33 0.22232021256386503 1107 34-35 0.08576664242200849 1107 36-37 0.018050441429245723 1107 38-39 0.10774059215442122 1107 40-41 0.09084693926715914 1107 42-43 0.16271136671192465 1107 44-45 0.2523379781735997 1107 46-47 0.23205644419655158 1107 48-49 0.18436198675998128 1107 50-51 0.16772421352056455 1107 52-53 0.08382972993529592 1107 54-55 -0.020225277157294386 1107 56-57 0.07624183348917057 1107 58-59 0.05041161075397582 1107 60-61 -0.016532035606971363 1107 62-63 0.029055810915892266 1107 64-65 0.01291846520199158 1107 66-67 -0.0955494993515984 1107 68-69 -0.08065475597881289 1107 70-71 0.01635326597811826 1107 72-73 0.045524609915347014 1107 74-75 0.10128481047731341 1107 76-77 0.23244592553927035 1107 78-79 0.28691857208041327 1107 80-81 0.10270828675870547 1107 82-83 0.2411731096619043 1107 84-85 0.21570122709204398 1107 86-87 0.2557554489746856 1107 88-89 0.29026300765292845 1107 90-91 0.3643010306083774 1107 92-93 0.2687315988566894 1107 94-95 0.27129188395440806 1107 96-97 0.3035893209323284 1107 98-99 0.648554189214078 1107 100-101 0.8307313927253936 1108 1 0.1323878521558406 1108 2 0.20346550271009178 1108 3 0.2007118830741632 1108 4 0.13275742829736714 1108 5 0.17405191515230456 1108 6 0.184885386347986 1108 7 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-1.834675065065099 2316 74-75 -1.9475362237606006 2316 76-77 -1.9418933670724705 2316 78-79 -1.537437657798339 2316 80-81 -1.7663179831623808 2316 82-83 -1.8055008950206997 2316 84-85 -1.9892053011905553 2316 86-87 -1.9745612911161352 2316 88-89 -2.1515891990749942 2316 90-91 -2.4671969188824896 2316 92-93 -2.5184349679460922 2316 94-95 -2.778500953473344 2316 96-97 -2.9714080174971436 2316 98-99 -3.2174530238620207 2316 100-101 -3.7095474518802405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6339.0 3 1520.0 4 1399.0 5 1662.0 6 1915.0 7 2353.0 8 2948.0 9 3768.0 10 4823.0 11 6588.0 12 9366.0 13 14442.0 14 14325.0 15 13876.0 16 17965.0 17 24301.0 18 20320.0 19 13637.0 20 13129.0 21 13864.0 22 14671.0 23 16230.0 24 18710.0 25 21619.0 26 25062.0 27 29887.0 28 34977.0 29 41411.0 30 49740.0 31 61669.0 32 76352.0 33 97386.0 34 131025.0 35 186236.0 36 279812.0 37 429551.0 38 408344.0 39 70528.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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21.72285013592479 18-19 20.237995561239412 28.911769597357388 30.471185332591627 20.37904950881157 20-21 22.264625235334044 26.510284272746397 29.431397157616633 21.793693334302926 22-23 20.97870443510578 26.906342050074095 29.848181832155955 22.266771682664167 24-25 22.238365422916164 26.664901418378527 30.003423419123788 21.093309739581517 26-27 22.025698460928457 25.711563022448868 31.555808266113395 20.706930250509284 28-29 20.516697484473127 28.302814947924826 30.552556883689952 20.627930683912098 30-31 21.075762501453983 27.047662922518644 30.026707424875188 21.849867151152186 32-33 21.00682212932933 27.237793545761402 30.2997336715224 21.455650653386872 34-35 22.294155847292757 26.54592307495889 29.99144479847658 21.168476279271776 36-37 21.873797734202242 26.14041934339489 31.115243940810895 20.870538981591977 38-39 21.53484383315007 26.97940522946164 30.801372784959934 20.68437815242836 40-41 21.335897709409775 27.43842467809562 30.288397849370707 20.9372797631239 42-43 20.599681707295154 27.892068999369034 29.219371701446423 22.28887759188939 44-45 21.46538545936666 26.886244908270502 29.206012896896745 22.4423567354661 46-47 22.251359218601994 27.693626710979075 28.794138593050295 21.260875477368636 48-49 21.971392728032203 27.258214655048807 29.062997502488518 21.707395114430472 50-51 20.802624427254987 27.937809077158477 30.059783538228412 21.199782957358128 52-53 21.57782403929427 28.727825456952854 28.254143078402976 21.440207425349904 54-55 20.55137827281306 27.34091098722936 29.23578544516988 22.8719252947877 56-57 21.895113383836755 27.41427429828111 28.375085142721783 22.315527175160355 58-59 22.904929459823702 27.095911552257153 28.180056769467527 21.819102218451622 60-61 21.60160044437341 27.29672380543556 29.89088256115316 21.21079318903787 62-63 21.49919613406861 27.30507633960754 29.506742517685975 21.68898500863787 64-65 20.986360439164212 28.639688786640917 28.854646218199314 21.519304555995557 66-67 21.33881787903487 27.389426186323146 28.27191848809152 22.999837446550465 68-69 20.741237928258045 27.838075641435942 28.83600323542514 22.584683194880874 70-71 21.966634955871577 27.483160461080804 28.180905551479622 22.369299031567998 72-73 21.52778498244022 26.597423520490864 29.465732204656682 22.409059292412234 74-75 20.753134062510878 28.8677260436174 28.334825332368435 22.04431456150329 76-77 21.34611935887926 27.13977757995223 28.276749438371628 23.23735362279688 78-79 21.312707020365515 27.20870694846364 29.03243350747261 22.446152523698238 80-81 21.85361116622571 27.439051509606188 28.507460519702644 22.199876804465458 82-83 21.420136153634477 27.022910298917562 29.261116009921356 22.2958375375266 84-85 21.11189121634797 27.359796572321056 28.821011025606865 22.707301185724106 86-87 21.239952964408477 27.56906105361753 28.656194440820848 22.534791541153147 88-89 21.255658924735148 27.241095549388238 28.136750048406277 23.366495477470334 90-91 21.36217100470474 26.364347247145524 28.764069499593337 23.509412248556394 92-93 21.917448179459637 27.347841032724286 28.671438626778446 22.06327216103763 94-95 21.504094927905616 26.998051154148794 28.580989842162623 22.916864075782968 96-97 20.277496258275367 26.82562963796336 29.548120469239475 23.348753634521803 98-99 21.343379967551787 27.24128176202508 28.021661959900612 23.393676310522522 100-101 21.438830513690224 26.072818594315994 28.951516890774414 23.53683400121937 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3553.0 1 2173.5 2 888.5 3 1003.5 4 881.5 5 872.5 6 1442.0 7 2566.5 8 3877.0 9 4376.0 10 3863.0 11 3541.0 12 3753.5 13 4019.5 14 4341.0 15 4667.5 16 5072.5 17 5300.5 18 5325.0 19 5549.5 20 6018.5 21 6653.5 22 7663.5 23 9033.0 24 10468.0 25 12652.5 26 15894.0 27 19029.0 28 21510.5 29 24290.0 30 27363.0 31 30840.0 32 34643.0 33 38655.5 34 42641.5 35 45906.5 36 50459.0 37 55210.0 38 57112.0 39 57484.5 40 58550.0 41 60540.0 42 62630.0 43 65048.0 44 68272.0 45 71232.0 46 77624.5 47 102141.5 48 158513.0 49 155418.0 50 96330.5 51 71488.5 52 63203.5 53 59157.0 54 55386.0 55 50572.5 56 45312.5 57 39566.5 58 35317.5 59 31770.5 60 26851.0 61 21966.0 62 17595.5 63 13258.0 64 9566.0 65 7269.0 66 5188.5 67 3777.0 68 2767.0 69 1770.5 70 1246.0 71 950.0 72 690.0 73 538.5 74 406.5 75 317.5 76 259.0 77 156.0 78 81.0 79 43.5 80 39.0 81 27.0 82 20.5 83 22.5 84 16.5 85 12.0 86 12.0 87 7.5 88 3.5 89 4.0 90 5.5 91 4.0 92 2.0 93 1.5 94 1.5 95 0.5 96 0.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2773893318776352 2 0.08456432870360822 3 0.08818524033915567 4 0.10147719444432986 5 0.10326473309985329 6 0.08066841112105716 7 0.08992694467017849 8 0.08635186735913164 9 0.09093529980919171 10-11 0.08474766600161063 12-13 0.08862066642191137 14-15 0.0971000164545225 16-17 0.08658103898163465 18-19 0.09664167320951648 20-21 0.09884172078554532 22-23 0.11094198245370389 24-25 0.12205680614509956 26-27 0.1251277058866398 28-29 0.11758795950629099 30-31 0.11066697650670028 32-33 0.10631271567914323 34-35 0.3776519167226991 36-37 0.581614660750372 38-39 0.5643351204136456 40-41 0.5281260040581711 42-43 0.5532202967222499 44-45 0.5759999559990484 46-47 0.5649538837944037 48-49 0.5872293655016956 50-51 0.5780625006015755 52-53 0.5821646726443793 54-55 0.572837387608507 56-57 0.5447409466896388 58-59 0.5393095792363176 60-61 0.4869438634943814 62-63 0.5616996467548611 64-65 0.5944482716105403 66-67 0.6074652197687108 68-69 0.6085423263944749 70-71 0.6056547639509371 72-73 0.5975191713520805 74-75 0.5949524491800469 76-77 0.5789791870915875 78-79 0.5567724568710466 80-81 0.5522806930699876 82-83 0.5642205346023941 84-85 0.5751291038335371 86-87 0.5660539075824181 88-89 0.5789333527670869 90-91 0.5910336144352455 92-93 0.598619195140095 94-95 0.5982066862195895 96-97 0.5951587036402995 98-99 0.5997192189281093 100-101 0.5781312520883264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2181771.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.0700642576476 #Duplication Level Percentage of deduplicated Percentage of total 1 72.69643200016286 35.67218589549701 2 14.466185086367789 14.197132635021816 3 5.495924026219069 8.090560353651572 4 2.6275244253238603 5.157311695567216 5 1.3932180514961936 3.4182649655916406 6 0.830627594771517 2.4455369669768166 7 0.5482156275741574 1.8830683250477356 8 0.36794367198575556 1.444401570202867 9 0.2778666714695969 1.22714418816646 >10 1.1897005981779947 9.939414312460906 >50 0.06486528637779482 2.189295681445651 >100 0.034567829337664814 2.910974605052149 >500 0.0023409225458560835 0.8306423018369212 >1k 0.0030900177605300222 3.4980893751216047 >5k 0.001030005920176674 3.478561607764558 >10k+ 4.6818450917121546E-4 3.6174155205951344 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 28888 1.324061966173352 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15282 0.7004401470181792 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12115 0.5552828413247769 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11656 0.5342448863790014 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10788 0.49446069271247994 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8872 0.406642126969329 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8363 0.3833124557985233 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7800 0.35750773110468514 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7117 0.3262028874707749 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6909 0.31666934797464996 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6766 0.31011503957106407 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6440 0.2951730497838682 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6195 0.2839436402812211 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5792 0.265472407507479 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5764 0.2641890464214622 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5689 0.26075147208391714 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4625 0.21198375081527807 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4357 0.19970015184911705 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3928 0.1800372266383594 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3642 0.1669286098311876 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3624 0.1661035919901768 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3588 0.16445355630815517 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3510 0.1608784789971083 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3193 0.1463489981304179 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3032 0.1389696718858212 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2993 0.13718213323029776 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2699 0.12370684182712118 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2679 0.12279015533710916 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2482 0.11376079341049083 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2453 0.11243159799997342 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2337 0.10711481635790374 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2183 0.10005633038481124 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.583432450060066E-5 0.0 3 0.0 0.0 0.0 4.583432450060066E-5 0.0 4 0.0 0.0 0.0 4.583432450060066E-5 0.0 5 0.0 0.0 0.0 1.3750297350180197E-4 0.0 6 4.583432450060066E-5 0.0 0.0 1.3750297350180197E-4 0.0 7 4.583432450060066E-5 0.0 0.0 1.3750297350180197E-4 0.0 8 4.583432450060066E-5 0.0 0.0 1.3750297350180197E-4 0.0 9 4.583432450060066E-5 0.0 0.0 1.8333729800240264E-4 0.0 10-11 4.583432450060066E-5 0.0 0.0 1.8333729800240264E-4 0.0 12-13 4.583432450060066E-5 0.0 0.0 2.7500594700360394E-4 0.0 14-15 4.583432450060066E-5 0.0 0.0 3.208402715042046E-4 0.0 16-17 4.583432450060066E-5 0.0 0.0 5.270947317569075E-4 0.0 18-19 4.583432450060066E-5 0.0 0.0 6.875148675090099E-4 0.0 20-21 4.583432450060066E-5 0.0 0.0 9.396036522623135E-4 0.0 22-23 4.583432450060066E-5 0.0 0.0 0.0013979468972683201 0.0 24-25 4.583432450060066E-5 0.0 0.0 0.00210837892702763 0.0 26-27 4.583432450060066E-5 0.0 0.0 0.0030250654170396437 0.0 28-29 4.583432450060066E-5 0.0 0.0 0.00831892989685902 0.0 30-31 4.583432450060066E-5 0.0 0.0 0.02326091968405483 0.0 32-33 4.583432450060066E-5 0.0 0.0 0.05364907682795307 0.0 34-35 4.583432450060066E-5 0.0 0.0 0.1049147687818749 0.0 36-37 4.583432450060066E-5 0.0 0.0 0.18381855840965894 0.0 38-39 4.583432450060066E-5 0.0 0.0 0.3007190030484409 0.0 40-41 4.583432450060066E-5 0.0 0.0 0.42825301097136226 0.0 42-43 4.583432450060066E-5 0.0 0.0 0.5588579186358238 0.0 44-45 4.583432450060066E-5 0.0 0.0 0.7132050063915965 0.0 46-47 4.583432450060066E-5 0.0 0.0 0.9020882576585718 0.0 48-49 4.583432450060066E-5 0.0 0.0 1.0990841843621535 0.0 50-51 4.583432450060066E-5 0.0 0.0 1.3131304797799586 0.0 52-53 4.583432450060066E-5 0.0 0.0 1.5205078809829262 0.0 54-55 4.583432450060066E-5 0.0 0.0 1.7543546045849907 0.0 56-57 4.583432450060066E-5 0.0 0.0 2.0488630566636004 0.0 58-59 4.583432450060066E-5 0.0 0.0 2.3589780962346643 0.0 60-61 4.583432450060066E-5 0.0 0.0 2.712773247054801 0.0 62-63 4.583432450060066E-5 0.0 0.0 3.06370375259365 0.0 64-65 4.583432450060066E-5 0.0 0.0 3.410279997304942 0.0 66-67 4.583432450060066E-5 0.0 0.0 3.815959603459758 0.0 68-69 4.583432450060066E-5 0.0 0.0 4.28005505619059 0.0 70-71 4.583432450060066E-5 0.0 0.0 4.774469914578569 0.0 72-73 4.583432450060066E-5 0.0 0.0 5.267211820122277 4.583432450060066E-5 74-75 4.583432450060066E-5 0.0 0.0 5.797537871756477 4.583432450060066E-5 76-77 4.583432450060066E-5 0.0 0.0 6.327772254741675 4.583432450060066E-5 78-79 4.583432450060066E-5 0.0 0.0 6.992163705540133 4.583432450060066E-5 80-81 4.583432450060066E-5 0.0 0.0 7.706743741666747 4.583432450060066E-5 82-83 4.583432450060066E-5 4.583432450060066E-5 0.0 8.377758252355541 4.583432450060066E-5 84-85 4.583432450060066E-5 4.583432450060066E-5 0.0 9.110053254901636 4.583432450060066E-5 86-87 4.583432450060066E-5 4.583432450060066E-5 0.0 9.862011182658492 4.583432450060066E-5 88-89 4.583432450060066E-5 4.583432450060066E-5 0.0 10.66381393830975 4.583432450060066E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10590 0.0 24.355999 1 TACATGG 10585 0.0 23.653208 2 ACATGGG 10725 0.0 22.505991 3 ACCGTCC 170 3.2508106E-6 22.272873 8 TTTGCGT 835 0.0 22.105654 3 CATGGGG 2985 0.0 21.722082 4 GAGTACT 8730 0.0 21.686821 12-13 GTCTACG 110 0.0029087458 21.55164 1 CTTTGCG 870 0.0 21.216347 2 AGTACTT 9015 0.0 20.7387 12-13 GTACCGG 145 6.2242924E-4 19.619423 1 GTACTTT 9775 0.0 19.441685 14-15 GTAAGCG 325 0.0 19.027533 94-95 CATGGGA 7690 0.0 18.586853 4 TACACCG 185 1.5645188E-4 17.908594 5 ATCGGAC 135 0.009600572 17.529175 3 TAGGACG 380 2.1827873E-10 17.436916 4 TTGCGTT 1075 0.0 17.170439 4 CGGGAGT 525 0.0 17.12851 4 AGTACAT 7980 0.0 17.021751 2 >>END_MODULE