##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939006_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2181771 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09858734028457 33.0 31.0 34.0 30.0 34.0 2 32.304957761378255 34.0 31.0 34.0 30.0 34.0 3 32.401927608351194 34.0 31.0 34.0 30.0 34.0 4 35.97395968687823 37.0 35.0 37.0 35.0 37.0 5 35.89376474432926 37.0 35.0 37.0 35.0 37.0 6 35.96354750338143 37.0 35.0 37.0 35.0 37.0 7 35.909756340147524 37.0 35.0 37.0 35.0 37.0 8 35.89320739894333 37.0 35.0 37.0 35.0 37.0 9 37.53947458280452 39.0 37.0 39.0 35.0 39.0 10-11 37.4596639610665 39.0 37.0 39.0 34.0 39.0 12-13 37.39539575876662 39.0 37.0 39.0 34.0 39.0 14-15 38.56260006205967 40.0 38.0 41.0 34.0 41.0 16-17 38.58982358826843 40.0 38.0 41.0 34.0 41.0 18-19 38.74381591835257 40.0 38.0 41.0 34.0 41.0 20-21 38.74680271210865 40.0 38.0 41.0 34.0 41.0 22-23 38.68939888741761 40.0 38.0 41.0 34.0 41.0 24-25 38.60555530346677 40.0 38.0 41.0 34.0 41.0 26-27 38.412038201992786 40.0 38.0 41.0 34.0 41.0 28-29 38.23547934224078 40.0 38.0 41.0 33.5 41.0 30-31 38.073704343856434 40.0 37.5 41.0 33.0 41.0 32-33 37.877212136379114 40.0 37.0 41.0 33.0 41.0 34-35 37.79692025423383 40.0 37.0 41.0 33.0 41.0 36-37 37.65979014296184 40.0 37.0 41.0 33.0 41.0 38-39 37.50314423466074 40.0 37.0 41.0 32.5 41.0 40-41 37.361009931839774 40.0 36.5 41.0 32.0 41.0 42-43 37.169439414127325 40.0 36.0 41.0 31.5 41.0 44-45 36.87382039636607 40.0 36.0 41.0 31.0 41.0 46-47 36.501281069369796 40.0 36.0 41.0 30.0 41.0 48-49 36.14472669221472 39.0 35.5 41.0 29.5 41.0 50-51 35.858560774710085 39.0 35.0 41.0 28.0 41.0 52-53 35.64159620785133 39.0 35.0 40.5 28.0 41.0 54-55 35.41374988484126 39.0 35.0 40.0 27.0 41.0 56-57 35.13291335341793 38.0 34.5 40.0 26.5 41.0 58-59 34.84860510108531 38.0 34.0 40.0 26.0 41.0 60-61 34.39432094385708 38.0 34.0 40.0 25.5 41.0 62-63 34.11491536004466 37.0 34.0 40.0 25.0 41.0 64-65 33.86321960462395 37.0 33.5 39.5 24.5 41.0 66-67 33.401068443938435 36.5 33.0 39.0 22.5 40.5 68-69 32.96907947717703 36.0 32.5 39.0 22.0 40.0 70-71 32.47201287394507 35.0 32.0 38.5 21.5 40.0 72-73 31.944125208374295 35.0 31.5 37.5 20.0 39.5 74-75 31.4890164916483 35.0 31.0 37.0 18.5 39.0 76-77 29.495242167945214 33.0 28.5 35.0 13.0 37.5 78-79 30.474553241380512 34.0 30.0 36.0 13.0 38.0 80-81 30.45577331443126 34.0 31.0 35.5 12.0 37.0 82-83 30.118758568154036 34.0 30.0 35.0 7.5 37.0 84-85 29.719283554506866 34.0 30.0 35.0 4.5 36.5 86-87 29.179832805551086 34.0 29.0 35.0 2.0 36.0 88-89 28.58841807870762 34.0 29.0 35.0 2.0 35.5 90-91 28.100056788728054 33.0 28.0 35.0 2.0 35.0 92-93 27.757907681420278 33.0 27.0 35.0 2.0 35.0 94-95 27.359380979947026 33.0 27.0 35.0 2.0 35.0 96-97 26.71637078318485 33.0 25.5 35.0 2.0 35.0 98-99 25.854798922526697 32.5 24.5 34.0 2.0 35.0 100-101 21.646577023894807 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.027724223134619308 1101 2 0.03490637932230811 1101 3 0.12236000001049518 1101 4 0.005410619866175637 1101 5 0.14517668247999893 1101 6 0.11827454467033505 1101 7 0.13203845649995571 1101 8 0.1235962217140667 1101 9 0.19951905832184025 1101 10-11 0.17032322681060208 1101 12-13 0.09982706737125824 1101 14-15 0.16903134642782192 1101 16-17 0.1413264967324963 1101 18-19 0.23388866171083578 1101 20-21 0.22057285224524037 1101 22-23 0.25852074991534124 1101 24-25 0.18636856914297795 1101 26-27 0.045363065273178904 1101 28-29 0.20749703503788908 1101 30-31 0.10186380006478402 1101 32-33 0.18168296625511715 1101 34-35 0.2370496319345463 1101 36-37 0.23612896549361295 1101 38-39 0.14385708157316657 1101 40-41 0.13468996804189004 1101 42-43 -1.0278191484758281E-4 1101 44-45 -0.17739603390103298 1101 46-47 -0.15320626228413659 1101 48-49 -0.36737637204720386 1101 50-51 -0.08435223315799334 1101 52-53 -0.19079190459087414 1101 54-55 -0.10314796905421986 1101 56-57 -0.2950300948232396 1101 58-59 -0.2683847093420795 1101 60-61 -0.1256092394124977 1101 62-63 -0.2520527614831849 1101 64-65 -0.0762713316038699 1101 66-67 -0.050614328279884546 1101 68-69 -0.22505909824854342 1101 70-71 -0.06978837316797382 1101 72-73 -0.15523890048582345 1101 74-75 -0.18617673623586484 1101 76-77 -0.3212589010187621 1101 78-79 -0.10425117065309664 1101 80-81 0.17221940751953824 1101 82-83 -0.1283551140847976 1101 84-85 -0.2806086392585918 1101 86-87 0.03564458102926338 1101 88-89 0.019265163113104222 1101 90-91 -0.19375557497899365 1101 92-93 -0.17306975982614503 1101 94-95 -0.11917533948151515 1101 96-97 -0.27609781465801575 1101 98-99 -0.24088892491655045 1101 100-101 0.495507553601616 1102 1 0.07968622862692598 1102 2 0.07156204681209033 1102 3 0.08918546274076533 1102 4 0.11599241076526567 1102 5 0.02465921127088677 1102 6 0.08337364652562229 1102 7 0.04366553341407098 1102 8 0.03467333978404241 1102 9 0.11868530614567874 1102 10-11 0.14764306976275066 1102 12-13 0.18114084984978973 1102 14-15 0.22292330683078632 1102 16-17 0.17889524158801606 1102 18-19 0.10937615834188108 1102 20-21 0.10749840020091739 1102 22-23 0.025148081777260245 1102 24-25 -0.0211115995596316 1102 26-27 0.152943027832265 1102 28-29 0.14340169912594547 1102 30-31 0.10275975950623462 1102 32-33 0.21076304171967308 1102 34-35 0.24869710467333306 1102 36-37 0.0647322885561934 1102 38-39 0.10683210397215248 1102 40-41 0.2449122825309331 1102 42-43 0.12927886038608705 1102 44-45 0.14607773925368406 1102 46-47 0.14811582420873748 1102 48-49 0.1501713214035476 1102 50-51 0.1914648745683749 1102 52-53 0.22941744398104902 1102 54-55 0.19806448988097003 1102 56-57 0.0986763364173342 1102 58-59 0.16019421157310632 1102 60-61 0.0789962106432256 1102 62-63 0.14570770412434797 1102 64-65 0.12466214158654765 1102 66-67 0.01340525171181639 1102 68-69 -0.03445101130404993 1102 70-71 0.13484730641361153 1102 72-73 0.20885024383648698 1102 74-75 0.17404375719740628 1102 76-77 -0.022007719153560146 1102 78-79 0.07095952550010765 1102 80-81 0.3792891102503937 1102 82-83 0.25671550645816765 1102 84-85 0.28333154270130123 1102 86-87 0.31934795289613405 1102 88-89 0.3600965114956409 1102 90-91 0.38548912392764123 1102 92-93 0.4804335872743657 1102 94-95 0.42967338905734564 1102 96-97 0.29523439690495934 1102 98-99 0.23034968437957204 1102 100-101 0.5440233931447516 1103 1 0.013393808279758446 1103 2 0.11520322188116694 1103 3 0.06978527520930555 1103 4 0.12417457567766377 1103 5 0.06983488751779987 1103 6 0.011492509961982478 1103 7 0.07634399573292683 1103 8 0.06223863954616604 1103 9 0.13076244767429301 1103 10-11 0.09424716029279523 1103 12-13 0.01605792097725356 1103 14-15 0.0849262812027689 1103 16-17 0.0812117163039332 1103 18-19 0.11082215369970783 1103 20-21 0.10500942920296552 1103 22-23 0.21311931222192015 1103 24-25 0.15445872858199294 1103 26-27 0.11082830795739085 1103 28-29 0.22961381335105102 1103 30-31 0.20406685957041049 1103 32-33 0.23880581657047628 1103 34-35 0.36687050786982667 1103 36-37 0.21957688338447667 1103 38-39 0.21664741141999144 1103 40-41 0.2913576630314978 1103 42-43 0.4371734354236523 1103 44-45 0.3994204251423241 1103 46-47 0.6407254081040819 1103 48-49 0.4611116014710319 1103 50-51 0.42972110095882243 1103 52-53 0.42562992327765414 1103 54-55 0.463176168133856 1103 56-57 0.37215391622388694 1103 58-59 0.23571609568788432 1103 60-61 0.25463006602879545 1103 62-63 0.4268570692842317 1103 64-65 0.40913750022125583 1103 66-67 0.5432034920671995 1103 68-69 0.6161764072946099 1103 70-71 0.5270918908661812 1103 72-73 0.5892203975123778 1103 74-75 0.6526522487604112 1103 76-77 0.40701998016587027 1103 78-79 0.5321957776761259 1103 80-81 0.5677787662377582 1103 82-83 0.5881830122128484 1103 84-85 0.40115992931585964 1103 86-87 0.43526703960253954 1103 88-89 0.6596787448109112 1103 90-91 0.924136265033539 1103 92-93 0.911041181530063 1103 94-95 0.8564960085029725 1103 96-97 0.6158176082275588 1103 98-99 0.6520105050987262 1103 100-101 1.0825822686092579 1104 1 -0.008064885533080712 1104 2 0.034447396405333564 1104 3 0.10197403053550858 1104 4 0.011139477995662617 1104 5 0.0920372489602812 1104 6 0.022416468160336933 1104 7 0.06912408354382649 1104 8 0.13486080874984907 1104 9 0.14649713984067603 1104 10-11 0.061970508312093386 1104 12-13 0.137069461403037 1104 14-15 0.1500336437505041 1104 16-17 0.11621030614622896 1104 18-19 0.16579519872566095 1104 20-21 0.06735202511274707 1104 22-23 0.03775333818406068 1104 24-25 0.07314836811520564 1104 26-27 0.08303843479536255 1104 28-29 0.10289685105942681 1104 30-31 -0.006318666113521942 1104 32-33 0.08589231640659278 1104 34-35 0.062526555159657 1104 36-37 0.12879607524977388 1104 38-39 0.18832063023835843 1104 40-41 0.25295825872785116 1104 42-43 0.07185302472475286 1104 44-45 0.11810468130319407 1104 46-47 0.1360235325943293 1104 48-49 0.05393189783282537 1104 50-51 0.07886242925115283 1104 52-53 -0.04415649499799201 1104 54-55 0.12269811514241269 1104 56-57 0.22442346024748616 1104 58-59 0.1982094736635105 1104 60-61 0.19836310602391904 1104 62-63 0.26560339144047873 1104 64-65 0.2940455255431189 1104 66-67 0.20311966390825376 1104 68-69 0.3556385750500155 1104 70-71 0.16677325239250962 1104 72-73 -0.009161446516561256 1104 74-75 0.052053217576869315 1104 76-77 0.07514412740157894 1104 78-79 0.27268650303076214 1104 80-81 0.46907082323861715 1104 82-83 0.47184523942355483 1104 84-85 0.3012757432817601 1104 86-87 0.29657564690029403 1104 88-89 0.2718820400064672 1104 90-91 0.47042088059405884 1104 92-93 0.42477306862597786 1104 94-95 0.5093909288862299 1104 96-97 0.2575165433679274 1104 98-99 0.18324668936441313 1104 100-101 0.8930787489318384 1105 1 -0.12598293222300683 1105 2 -0.05677487124088998 1105 3 -0.043191650696883244 1105 4 -0.10740471243324379 1105 5 0.0020031317398974124 1105 6 -0.028495892941414525 1105 7 -0.10089032191492464 1105 8 0.026973520159870645 1105 9 -0.1726705181048871 1105 10-11 -0.21926031401719825 1105 12-13 -0.1439172709941019 1105 14-15 -0.22234423514951374 1105 16-17 -0.07321732372668777 1105 18-19 -0.07344431117497408 1105 20-21 -0.0586452137640876 1105 22-23 -0.15855402871770963 1105 24-25 -0.08215535081870229 1105 26-27 -0.05849704314564974 1105 28-29 0.0064794821920131085 1105 30-31 -0.07010642507263753 1105 32-33 -0.07723289296166769 1105 34-35 -0.08149942449920644 1105 36-37 -0.15163810952215329 1105 38-39 -0.12919353759031083 1105 40-41 -0.0991541573748762 1105 42-43 -0.07508796088178826 1105 44-45 -0.040663822697759144 1105 46-47 -0.02924219321100452 1105 48-49 -0.0015754908478626817 1105 50-51 -0.1566172190009425 1105 52-53 0.09199008631257044 1105 54-55 0.04393620018537803 1105 56-57 -0.02221234373122627 1105 58-59 -0.11593888318213175 1105 60-61 -0.11675076723572886 1105 62-63 -0.08728674409633896 1105 64-65 -0.06106495168268822 1105 66-67 0.1255302111772636 1105 68-69 -0.05963384131111127 1105 70-71 -0.05766121107567557 1105 72-73 -0.04562728269299399 1105 74-75 -0.044612393981459775 1105 76-77 -0.19258838325486138 1105 78-79 -0.1676254337513292 1105 80-81 -0.18378322943866365 1105 82-83 -0.0706707048630335 1105 84-85 -0.17268922773660123 1105 86-87 -0.308668410732615 1105 88-89 -0.08284084640081346 1105 90-91 0.05539832188558691 1105 92-93 0.10536385854529584 1105 94-95 0.03228162735257101 1105 96-97 -0.3385436882452737 1105 98-99 -0.13508026563906128 1105 100-101 0.5318484895320701 1106 1 -0.13164401879114607 1106 2 -0.143640317124202 1106 3 -0.2154509452631359 1106 4 -0.13928395949356087 1106 5 -0.09649855179991107 1106 6 -0.13614575289173558 1106 7 -0.08794121757621554 1106 8 -0.047392067244935276 1106 9 -0.05044371687580451 1106 10-11 0.08038101388473251 1106 12-13 -1.4693435407764355E-4 1106 14-15 -0.017346148520239524 1106 16-17 -0.14794271540160508 1106 18-19 -0.1695826988261473 1106 20-21 -0.0964075663269881 1106 22-23 0.027505989985769475 1106 24-25 0.02245417437098496 1106 26-27 -0.03360669400643701 1106 28-29 -0.018838035452887425 1106 30-31 -0.05783651073276985 1106 32-33 -0.07405024417727901 1106 34-35 -0.04237982411064678 1106 36-37 -0.19971024699501783 1106 38-39 -0.1226524137057865 1106 40-41 0.045230495806862336 1106 42-43 -0.06387300443644506 1106 44-45 -0.051599245869695665 1106 46-47 -0.12401019984100259 1106 48-49 -0.27479769772286033 1106 50-51 -0.3103138738854767 1106 52-53 -0.1884366972546374 1106 54-55 -0.16310885346825899 1106 56-57 -0.23142177064838876 1106 58-59 -0.15596580623292766 1106 60-61 -0.13816817868288922 1106 62-63 -0.1261673429700565 1106 64-65 -0.22007783353601695 1106 66-67 -0.2352268949004639 1106 68-69 -0.14250453141524844 1106 70-71 -0.19043691194676882 1106 72-73 -0.12587325838752506 1106 74-75 -0.14424092833948166 1106 76-77 -0.3081309540552404 1106 78-79 -0.05377138431164141 1106 80-81 0.02665211334905848 1106 82-83 0.25977962788232745 1106 84-85 0.058672390273084574 1106 86-87 0.08262405420967767 1106 88-89 0.11963091156797745 1106 90-91 0.38157028975907537 1106 92-93 0.2391257944688867 1106 94-95 0.2645343779645124 1106 96-97 0.24010501915392624 1106 98-99 0.3008365333030376 1106 100-101 0.6215365534952646 1107 1 -0.157686311200667 1107 2 -0.13384152847051212 1107 3 -0.15512611927705677 1107 4 -0.04926957544497412 1107 5 -0.07487618543103025 1107 6 -0.04973344323237683 1107 7 -0.1508937502158858 1107 8 -0.08543980465062617 1107 9 -0.14301306066558794 1107 10-11 -0.007098250489669056 1107 12-13 -0.07021598880954372 1107 14-15 -0.007349510309367702 1107 16-17 -0.11053426904418728 1107 18-19 -0.013660714378559646 1107 20-21 -0.04617692554793962 1107 22-23 -0.08421452960354259 1107 24-25 -0.2046231082011687 1107 26-27 -0.16439902912721038 1107 28-29 -0.13718789299018397 1107 30-31 -0.22597611867099943 1107 32-33 -0.1954383875327821 1107 34-35 -0.14783914921769536 1107 36-37 -0.04076210121461088 1107 38-39 -0.2706031001169933 1107 40-41 -0.14340390135821224 1107 42-43 -0.12486412600645025 1107 44-45 -0.23022873812931266 1107 46-47 -0.4076140377917383 1107 48-49 -0.3712849036351997 1107 50-51 -0.3621719512727637 1107 52-53 -0.2120210258061448 1107 54-55 -0.2282746558060751 1107 56-57 -0.1712672385708629 1107 58-59 -0.18522163838596128 1107 60-61 -0.34666578960815286 1107 62-63 -0.3127152264206856 1107 64-65 -0.35043589163434063 1107 66-67 -0.21052821591825932 1107 68-69 -0.3371107887519358 1107 70-71 -0.2805708875104145 1107 72-73 -0.19331129951157777 1107 74-75 -0.35213176388186085 1107 76-77 -0.7710355398662863 1107 78-79 -0.5314452466766859 1107 80-81 -0.37336993116984374 1107 82-83 -0.3455077499772621 1107 84-85 -0.30444768071026473 1107 86-87 -0.25648237121646744 1107 88-89 -0.012965216312274208 1107 90-91 0.12130773953889928 1107 92-93 0.19985679071580975 1107 94-95 0.04605996889539554 1107 96-97 -0.036212554185432566 1107 98-99 -0.03528065535946823 1107 100-101 0.42331168838016353 1108 1 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2316 90-91 -1.992730835196678 2316 92-93 -2.195620827015798 2316 94-95 -2.453805940741425 2316 96-97 -2.730551371673844 2316 98-99 -3.1373273810998015 2316 100-101 -3.6776358307281534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 19.0 5 58.0 6 135.0 7 368.0 8 735.0 9 1421.0 10 2553.0 11 3901.0 12 5878.0 13 8126.0 14 9639.0 15 12813.0 16 16836.0 17 23839.0 18 22541.0 19 14503.0 20 12722.0 21 12938.0 22 13946.0 23 15377.0 24 17696.0 25 21041.0 26 24982.0 27 30104.0 28 36531.0 29 44512.0 30 54169.0 31 65815.0 32 81417.0 33 103241.0 34 137911.0 35 196936.0 36 304615.0 37 477911.0 38 377421.0 39 29110.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.37897705205302 19.725119783239734 14.49708525916068 21.398817905546565 2 15.146502543117496 22.75023364046914 38.697095158016126 23.406168658397238 3 21.432496811076874 25.709114292929918 30.790215838417506 22.068173057575706 4 12.485636668559625 16.915936640463183 38.80631835330106 31.792108337676133 5 12.271297655202254 39.38259190032295 34.49966678369724 13.846443660777554 6 29.560343409092887 37.337740761977315 17.34210418966977 15.759811639260032 7 26.132119273745964 34.8033776230411 21.40632541178703 17.658177691425912 8 25.070229643716047 33.366059041026766 20.294934711296463 21.268776603960728 9 25.266125546631613 18.339825765398842 22.277406748920946 34.1166419390486 10-11 24.564493707176418 26.38216843105899 27.54143308349043 21.511904778274165 12-13 27.399438346187573 24.459991447315048 27.389698552231188 20.75087165426619 14-15 22.921967754717496 25.89403759512739 28.455977106404024 22.728017543751093 16-17 20.50600339318736 29.026510909145493 28.79334673099193 21.674138966675216 18-19 20.187567806153808 28.99105818163318 30.454410659963855 20.366963352249158 20-21 22.160827144553668 26.538348891794783 29.566256036953465 21.73456792669808 22-23 20.97055557159757 26.960299683147316 29.872200152994978 22.19694459226014 24-25 22.090586042256497 26.796258635759667 30.097613360888932 21.015541961094907 26-27 21.928676290958123 25.81464782509255 31.642367599532673 20.614308284416648 28-29 20.428381347079963 28.398168277055657 30.610086943130142 20.56336343273423 30-31 21.03096521128936 27.105984083572476 30.104121834968012 21.75892887017015 32-33 20.963520002786726 27.305844655557344 30.437383208411884 21.29325213324405 34-35 22.192808603128363 26.57394310355417 30.16980196340682 21.063446329910647 36-37 21.864038409927286 26.179471389082487 31.202725859892873 20.75376434109735 38-39 21.372574725900364 27.03274710223121 30.989417402473336 20.605260769395088 40-41 21.254927862193572 27.532890228704144 30.284196877061152 20.92798503204113 42-43 20.594167502956903 27.96922757491262 29.281471803593295 22.15513311853718 44-45 21.490963617275657 26.95104212715998 29.225280375256073 22.33271388030829 46-47 22.248121245010918 27.77658353515779 28.80361945654984 21.171675763281456 48-49 21.958592708519774 27.392219741305833 29.049341003879658 21.59984654629473 50-51 20.750142602859114 28.08727455398936 29.93933563224344 21.22324721090808 52-53 21.54575342691786 28.904522976976043 28.102674387000288 21.447049209105813 54-55 20.52726890219001 27.423180526278877 29.118615106718348 22.93093546481276 56-57 21.883850321596537 27.5392330359144 28.256425628537553 22.32049101395151 58-59 22.841348319962673 27.2605644874086 28.054324774368666 21.843762418260063 60-61 21.541774723315392 27.50645589776168 29.698436008921377 21.25333337000155 62-63 21.420969478464972 27.493421628576055 29.285085373304533 21.800523519654448 64-65 20.894147013440715 28.8042968851158 28.627467696474397 21.674088404969094 66-67 21.321340109600996 27.519282845005712 28.055737368292714 23.103639677100578 68-69 20.77066241133484 27.983550694894678 28.52121947983106 22.72456741393942 70-71 21.937178558152986 27.65116045634487 27.86426714811041 22.547393837391734 72-73 21.50496087811232 26.778108243257424 29.066501479761165 22.650429398869086 74-75 20.669699065575625 29.020552569449315 27.88936144077449 22.42038692420057 76-77 21.212950714914246 27.180439698362747 28.0309025598783 23.575707026844704 78-79 21.194626381332586 27.273887715020372 28.678297895745196 22.853188007901842 80-81 21.705050621719696 27.54505399512598 28.1188080692245 22.63108731392983 82-83 21.252803341872266 27.177921055876165 28.812212647431835 22.75706295481973 84-85 20.843617409893156 27.43463910740403 28.432383600295356 23.289359882407457 86-87 20.958655934608867 27.637580990744702 28.144478859327176 23.259284215319255 88-89 20.931370574499404 27.296104443137576 27.667787223807217 24.104737758555807 90-91 21.03631820513581 26.394385323995163 28.229690705814697 24.33960576505433 92-93 21.54073456838504 27.454004109505536 28.04888780719883 22.956373514910595 94-95 21.057159527741454 27.008471558197446 28.050950351801358 23.88341856225974 96-97 19.729305946294783 27.014701878573373 28.964708341239785 24.291283833892056 98-99 20.753392542113723 27.331832717549183 27.38974438655569 24.525030353781403 100-101 20.667270610974537 26.212726052282814 28.410186506955252 24.709816829787396 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1676.0 1 1153.5 2 732.0 3 852.0 4 753.0 5 746.0 6 1195.0 7 2240.5 8 3397.5 9 3899.0 10 3500.0 11 3078.0 12 3211.0 13 3424.5 14 3676.5 15 3857.5 16 4070.0 17 4334.0 18 4659.0 19 5105.0 20 5621.0 21 6397.0 22 7520.5 23 8940.5 24 10490.0 25 12759.0 26 15995.0 27 19182.0 28 21630.0 29 24365.5 30 27477.0 31 31222.0 32 35138.0 33 38897.0 34 42825.5 35 46054.0 36 50185.0 37 54702.0 38 56788.0 39 57182.5 40 58251.0 41 60286.0 42 62212.5 43 64906.0 44 68257.5 45 71137.5 46 76176.0 47 101382.0 48 157199.0 49 151429.5 50 95362.5 51 75558.0 52 69536.0 53 63925.5 54 57820.0 55 52182.5 56 46528.0 57 40612.0 58 36236.5 59 32160.5 60 27103.5 61 22123.5 62 17494.0 63 12984.5 64 9283.5 65 6946.5 66 4905.0 67 3631.0 68 2512.5 69 1545.0 70 1104.5 71 818.0 72 594.0 73 480.0 74 362.0 75 273.5 76 191.0 77 84.0 78 34.5 79 13.5 80 9.0 81 10.0 82 7.5 83 3.0 84 1.5 85 1.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39779610234071316 2 0.0 3 0.0 4 0.0 5 2.2917162250300332E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011687752747653168 16-17 0.002131296089277931 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002429219198531835 36-37 0.006760562863838597 38-39 0.012146095992659174 40-41 0.002016710278026429 42-43 5.729290562575082E-4 44-45 3.4375743375450495E-4 46-47 3.208402715042046E-4 48-49 2.520887847533036E-4 50-51 5.729290562575082E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.8333729800240264E-4 60-61 0.0020625446025270295 62-63 0.0 64-65 2.0625446025270298E-4 66-67 0.0017875386555234257 68-69 0.0017187871687725247 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12207972330734984 78-79 4.583432450060066E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14332393271337826 88-89 0.43489898802394933 90-91 0.33255094141410807 92-93 0.0 94-95 0.0 96-97 4.1250892050540596E-4 98-99 0.0 100-101 1.1458581125150166E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2181771.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.81986802269349 #Duplication Level Percentage of deduplicated Percentage of total 1 71.59708727003859 34.23763264062513 2 15.015846757947205 14.361116204280505 3 5.703895871311242 8.182786433438697 4 2.668232721150433 5.103781463169841 5 1.4746304609520942 3.5258317012486398 6 0.8924480591608837 2.5606049043709445 7 0.5911257337260839 1.9787288200119428 8 0.3901091126848398 1.492397302643128 9 0.29375085365482245 1.2642414347994444 >10 1.259926360610468 10.25100544799259 >50 0.06852853559161834 2.2644195816625476 >100 0.03703383890483465 3.069389637997621 >500 0.0025893435740953663 0.8664334556743076 >1k 0.0032606548710830455 3.622758548904222 >5k 0.0010549177524092205 3.6459637216439242 >10k+ 4.7950806927691837E-4 3.5729087015365217 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 30324 1.3898800561562143 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14229 0.6521766033190468 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11443 0.5244821752603733 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11276 0.516827843068773 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10637 0.4875397097128892 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9601 0.4400553495302669 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8401 0.38505416012954613 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8157 0.3738705849513996 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7338 0.33633227318540765 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7311 0.3350947464238914 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7295 0.3343613972318818 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6952 0.31864022392817576 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6609 0.3029190506244697 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6232 0.2856395102877433 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6005 0.27523511862610694 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5601 0.25671805152786425 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4960 0.22733824952297926 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4403 0.20180853077614472 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3866 0.17719549851932215 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3800 0.1741704331022825 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3592 0.16463689360615757 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3581 0.16413271603665094 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3432 0.15730340168606144 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3264 0.14960323516996055 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3156 0.1446531281238957 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2822 0.12934446374069505 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2786 0.12769442805867343 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2694 0.12347767020461818 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2621 0.12013176451607434 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2601 0.11921507802606232 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2522 0.11559416639051487 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2240 0.10266888688134548 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.583432450060066E-5 0.0 2 0.0 0.0 0.0 4.583432450060066E-5 0.0 3 0.0 0.0 0.0 4.583432450060066E-5 0.0 4 0.0 0.0 0.0 1.3750297350180197E-4 0.0 5 0.0 0.0 0.0 1.3750297350180197E-4 0.0 6 0.0 0.0 0.0 1.3750297350180197E-4 0.0 7 0.0 0.0 0.0 1.8333729800240264E-4 0.0 8 0.0 0.0 0.0 1.8333729800240264E-4 0.0 9 0.0 0.0 0.0 2.291716225030033E-4 0.0 10-11 4.583432450060066E-5 0.0 0.0 2.520887847533036E-4 0.0 12-13 4.583432450060066E-5 0.0 0.0 3.895917582551056E-4 0.0 14-15 4.583432450060066E-5 0.0 0.0 4.5834324500600653E-4 0.0 16-17 4.583432450060066E-5 2.291716225030033E-5 0.0 6.645977052587096E-4 0.0 18-19 4.583432450060066E-5 4.583432450060066E-5 0.0 7.791835165102113E-4 0.0 20-21 4.583432450060066E-5 4.583432450060066E-5 0.0 0.0010312723012635147 0.0 22-23 4.583432450060066E-5 4.583432450060066E-5 0.0 0.0015125327085198218 0.0 24-25 4.583432450060066E-5 4.583432450060066E-5 0.0 0.0022687990627797325 0.0 26-27 4.583432450060066E-5 4.583432450060066E-5 0.0 0.0032313198772923463 0.0 28-29 4.583432450060066E-5 4.583432450060066E-5 0.0 0.008502267194861421 0.0 30-31 4.583432450060066E-5 4.583432450060066E-5 0.0 0.02367342860456024 0.0 32-33 4.583432450060066E-5 4.583432450060066E-5 0.0 0.05417617155970998 0.0 34-35 4.583432450060066E-5 4.583432450060066E-5 0.0 0.10622104703014203 0.0 36-37 4.583432450060066E-5 4.583432450060066E-5 0.0 0.18615610895918958 0.0 38-39 4.583432450060066E-5 4.583432450060066E-5 0.0 0.3051190982004986 0.0 40-41 4.583432450060066E-5 4.583432450060066E-5 0.0 0.4350364909974511 0.0 42-43 4.583432450060066E-5 4.583432450060066E-5 0.0 0.5676351917776888 0.0 44-45 4.583432450060066E-5 4.583432450060066E-5 0.0 0.7248010904902485 0.0 46-47 4.583432450060066E-5 4.583432450060066E-5 0.0 0.916296898253758 0.0 48-49 4.583432450060066E-5 4.583432450060066E-5 0.0 1.1152407837486153 0.0 50-51 6.875148675090098E-5 4.583432450060066E-5 0.0 1.3309600320106922 0.0 52-53 9.166864900120132E-5 4.583432450060066E-5 0.0 1.5402395576804349 0.0 54-55 9.166864900120132E-5 4.583432450060066E-5 0.0 1.775965488587024 0.0 56-57 9.166864900120132E-5 4.583432450060066E-5 0.0 2.072261479321157 0.0 58-59 9.166864900120132E-5 4.583432450060066E-5 0.0 2.3837744657894895 0.0 60-61 9.166864900120132E-5 4.583432450060066E-5 0.0 2.7388300605333926 0.0 62-63 9.166864900120132E-5 4.583432450060066E-5 0.0 3.092556459866778 0.0 64-65 9.166864900120132E-5 4.583432450060066E-5 0.0 3.4409660775580937 0.0 66-67 9.166864900120132E-5 4.583432450060066E-5 0.0 3.846554015063909 0.0 68-69 9.166864900120132E-5 4.583432450060066E-5 0.0 4.313399527264776 0.0 70-71 9.166864900120132E-5 4.583432450060066E-5 0.0 4.810174853364537 0.0 72-73 9.166864900120132E-5 4.583432450060066E-5 0.0 5.3075460256828055 0.0 74-75 9.166864900120132E-5 4.583432450060066E-5 0.0 5.842295089631314 0.0 76-77 9.166864900120132E-5 9.166864900120132E-5 0.0 6.378281680341337 0.0 78-79 9.166864900120132E-5 9.166864900120132E-5 0.0 7.045996119666087 0.0 80-81 9.166864900120132E-5 9.166864900120132E-5 0.0 7.7643345704017515 0.0 82-83 9.166864900120132E-5 9.166864900120132E-5 0.0 8.438465815156587 0.0 84-85 9.166864900120132E-5 9.166864900120132E-5 0.0 9.173144202576715 0.0 86-87 9.166864900120132E-5 9.166864900120132E-5 0.0 9.926843834664592 0.0 88-89 9.166864900120132E-5 9.166864900120132E-5 0.0 10.730113288699869 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 805 0.0 28.883883 3 TTGCGTT 855 0.0 27.749762 4 CTTTGCG 830 0.0 27.442173 2 CACGCGA 55 9.544886E-5 25.884144 94-95 TACATGG 10680 0.0 24.125921 2 GTCGGGA 395 0.0 24.026377 2 GTACATG 10915 0.0 23.7452 1 ACATGGG 10800 0.0 23.154863 3 GAGTACT 9965 0.0 22.454302 12-13 GTAAGCG 290 0.0 22.090778 94-95 ATGGGCG 215 4.4736225E-8 22.070742 5 AGTACTT 10230 0.0 20.55054 12-13 CATGGGA 7605 0.0 20.528254 4 GTACTTT 10955 0.0 20.273495 14-15 GATCGTA 150 7.776691E-4 18.980837 7 ATACCGT 200 1.2968334E-5 18.980837 6 CGGGAGT 540 0.0 18.45359 4 GTGTAGC 1190 0.0 18.416687 1 CATGGGG 3195 0.0 18.41646 4 ATGGGAG 3145 0.0 18.105726 5 >>END_MODULE