FastQCFastQC Report
Fri 27 May 2016
SRR939005_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939005_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028434
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103570.3419919337849199No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT98520.3253166488026485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80550.26597905055880366No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52350.17286161758849622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50110.1654650555369541No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41530.13713358125024352No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38180.12607175853923183No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG135950.024.6119981
TACATGG140050.023.3682672
ACATGGG140850.022.4285143
GAGTACT94000.020.89901212-13
CATGGGG63150.019.799864
GTATCAA255050.019.5761551
GTACTTT102550.019.11152314-15
AGTACTT99650.018.97776212-13
CATGGGA80800.017.9952774
AGTACAT103100.017.6861632
CAACGCA285800.017.1680875
TCAACGC286750.017.1114924
ATCAACG286200.017.1112793
TATCAAC287900.017.1089442
AACGCAG290700.016.9189136
GGTATCA205900.016.8729781
AGAGTAC194900.016.7946710-11
ACTTTTT125200.015.99620216-17
GAGTACA141550.015.6247481
TACTTTT121200.015.54572714-15
Produced by FastQC (version 0.11.5)