FastQCFastQC Report
Fri 27 May 2016
SRR939005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028434
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT106240.35080837158742767No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102570.3386898971547671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84630.27945136000982684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51470.1699558253539618No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49000.16179979487748453No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38510.12716143062718221No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35800.1182129113594683No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT5050.025.3704763
GTACATG139550.024.1680011
TACATGG143150.023.071412
TACCGTA1503.059018E-522.1443547
ACATGGG143250.021.8959123
GAGTACT99550.021.54747612-13
CTTTGCG6150.020.8326662
GTACTTT104500.020.25432814-15
GTATAGG7500.019.6896381
CATGGGG61600.019.489294
GTATCAA269900.018.9203531
AGTACTT102900.018.90896412-13
TGTACCG3409.0403773E-1018.1434825
GGTATCA219100.018.0456711
CATGGGA85500.017.9818824
TCTATAC8700.017.4536783
AGTACAT108550.017.3109782
ACCGTAT1952.2944383E-417.0341198
AGAGTAC203150.016.8195710-11
ACTTTTT129300.016.7354316-17
Produced by FastQC (version 0.11.5)