##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939005_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3028434 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.219280657924195 33.0 31.0 34.0 31.0 34.0 2 32.45353473115148 34.0 31.0 34.0 31.0 34.0 3 32.54424167738178 34.0 31.0 34.0 31.0 34.0 4 36.08038279850246 37.0 35.0 37.0 35.0 37.0 5 36.005820169764306 37.0 35.0 37.0 35.0 37.0 6 36.0489751468911 37.0 35.0 37.0 35.0 37.0 7 35.9878488354047 37.0 35.0 37.0 35.0 37.0 8 35.96323512415988 37.0 35.0 37.0 35.0 37.0 9 37.64596619903224 39.0 37.0 39.0 35.0 39.0 10-11 37.55784788441815 39.0 37.0 39.0 35.0 39.0 12-13 37.46591423157975 39.0 37.0 39.0 34.0 39.0 14-15 38.79128090623735 40.0 38.0 41.0 34.5 41.0 16-17 38.881131469267615 40.0 38.0 41.0 35.0 41.0 18-19 38.91723676329086 40.0 38.0 41.0 35.0 41.0 20-21 38.89134301754636 40.0 38.0 41.0 35.0 41.0 22-23 38.818206373326944 40.0 38.0 41.0 34.0 41.0 24-25 38.73777420937686 40.0 38.0 41.0 34.0 41.0 26-27 38.58203299130838 40.0 38.0 41.0 34.0 41.0 28-29 38.42759029914471 40.0 38.0 41.0 34.0 41.0 30-31 38.239944803155694 40.0 38.0 41.0 34.0 41.0 32-33 38.154716265898486 40.0 38.0 41.0 33.0 41.0 34-35 38.062547673153844 40.0 38.0 41.0 33.0 41.0 36-37 37.930349811156525 40.0 37.0 41.0 33.0 41.0 38-39 37.793664151175165 40.0 37.0 41.0 33.0 41.0 40-41 37.62916593196351 40.0 37.0 41.0 33.0 41.0 42-43 37.43780250783078 40.0 37.0 41.0 32.0 41.0 44-45 37.18101120909354 40.0 36.5 41.0 31.5 41.0 46-47 36.860980295426614 40.0 36.0 41.0 31.0 41.0 48-49 36.53047796319814 39.5 36.0 41.0 30.0 41.0 50-51 36.25989620378057 39.0 35.0 41.0 30.0 41.0 52-53 35.998666967812404 39.0 35.0 40.0 29.0 41.0 54-55 35.698088021730044 38.5 35.0 40.0 28.0 41.0 56-57 35.55858143185554 38.0 35.0 40.0 28.0 41.0 58-59 35.277214725498396 38.0 34.5 40.0 28.0 41.0 60-61 34.90575327050218 38.0 34.0 40.0 27.0 41.0 62-63 34.490025207747635 37.0 34.0 40.0 26.0 41.0 64-65 34.07996046801746 36.5 33.5 39.5 26.0 41.0 66-67 33.625473924807345 36.0 33.0 39.0 25.5 40.5 68-69 33.139639496848865 35.5 32.5 39.0 24.5 40.0 70-71 32.61038048047274 35.0 32.0 38.0 23.0 40.0 72-73 32.1024415589047 35.0 31.0 37.0 22.0 39.0 74-75 31.58324005079853 35.0 31.0 37.0 20.5 39.0 76-77 29.67898227268615 32.5 28.5 35.0 18.0 37.0 78-79 30.641228271773464 34.0 30.0 35.5 18.5 37.5 80-81 30.628466230401585 34.0 31.0 35.0 18.0 37.0 82-83 30.223765979380765 34.0 30.0 35.0 15.5 37.0 84-85 29.781569781609903 34.0 30.0 35.0 9.0 36.0 86-87 29.09284518005015 34.0 29.0 35.0 4.5 36.0 88-89 28.41970173363527 33.0 28.5 35.0 2.0 35.0 90-91 27.846382651892036 33.0 27.0 35.0 2.0 35.0 92-93 27.44033005176933 33.0 26.0 35.0 2.0 35.0 94-95 26.897966242619123 32.5 25.5 34.0 2.0 35.0 96-97 26.058762713666535 32.0 24.5 34.0 2.0 35.0 98-99 24.86483558829415 32.0 12.5 34.0 2.0 35.0 100-101 19.319198800436133 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10036301638518808 1101 2 0.15928877666567587 1101 3 0.18239512395528124 1101 4 0.07473063179325834 1101 5 0.10104629040127833 1101 6 0.07186825881303349 1101 7 0.14249834527907268 1101 8 0.18278492388792245 1101 9 0.15227331865779092 1101 10-11 0.2207496310558028 1101 12-13 0.17958290712996217 1101 14-15 0.2697281910092002 1101 16-17 0.19427368151688285 1101 18-19 0.23606967811294766 1101 20-21 0.2884275490223729 1101 22-23 0.2686976560475429 1101 24-25 0.2065014226300903 1101 26-27 0.1170500292125638 1101 28-29 0.28677715927402403 1101 30-31 0.2314827463107889 1101 32-33 0.32522365567541556 1101 34-35 0.2653272091464345 1101 36-37 0.38441459227335883 1101 38-39 0.25708826442814825 1101 40-41 0.26456563680761036 1101 42-43 0.2634764901812119 1101 44-45 0.38391969102431034 1101 46-47 0.3200452525294466 1101 48-49 0.2246162586338798 1101 50-51 0.06663981927160023 1101 52-53 -0.03425224573405927 1101 54-55 -0.02880629503199117 1101 56-57 0.0025777647558484773 1101 58-59 -0.01406562203630557 1101 60-61 0.04875768026012395 1101 62-63 0.04534943113370815 1101 64-65 0.0474406638647622 1101 66-67 5.497662179934082E-4 1101 68-69 0.128024184085767 1101 70-71 -0.007658892403696882 1101 72-73 0.13997681839143183 1101 74-75 0.19943249635193894 1101 76-77 -0.1178967292943831 1101 78-79 0.29613922658890957 1101 80-81 0.32458757069295174 1101 82-83 0.1732232185118825 1101 84-85 0.23490566950772873 1101 86-87 0.3397033228304345 1101 88-89 0.3894750282004331 1101 90-91 0.285044173346229 1101 92-93 0.19872838207631105 1101 94-95 0.12565451698591446 1101 96-97 2.4873983639750463E-4 1101 98-99 0.3610179797911428 1101 100-101 0.787795910308553 1102 1 0.08223587366056506 1102 2 0.049401433577955345 1102 3 0.14657056660361434 1102 4 0.115786702791155 1102 5 0.03752793419644007 1102 6 0.07667004436166991 1102 7 0.16592799197442076 1102 8 0.04944994988930773 1102 9 0.2026073517979654 1102 10-11 0.12844801115125648 1102 12-13 0.15908658064261516 1102 14-15 0.2900399426528608 1102 16-17 0.233946387724842 1102 18-19 0.1965223881449205 1102 20-21 0.17124713326516883 1102 22-23 0.13560878184050296 1102 24-25 0.25369200420175986 1102 26-27 0.35315201675234675 1102 28-29 0.25876248415415404 1102 30-31 0.1599088813514271 1102 32-33 0.22671085564663684 1102 34-35 0.22438850881836458 1102 36-37 0.3435544545710627 1102 38-39 0.3521854833111959 1102 40-41 0.3230427334358623 1102 42-43 0.33348714819525327 1102 44-45 0.4312214907710157 1102 46-47 0.6605351993529567 1102 48-49 0.6379815596991207 1102 50-51 0.5624897249018019 1102 52-53 0.6573241370154861 1102 54-55 0.6617071259254956 1102 56-57 0.5667260417733431 1102 58-59 0.48793583184554024 1102 60-61 0.5315529384850635 1102 62-63 0.519793387585402 1102 64-65 0.3545268234017982 1102 66-67 0.2722846372333052 1102 68-69 0.25481432343504196 1102 70-71 0.4167605487226069 1102 72-73 0.43005606252431505 1102 74-75 0.4245352430149154 1102 76-77 0.21614019703280718 1102 78-79 0.4925566761999782 1102 80-81 0.5329943395730687 1102 82-83 0.5133146729805809 1102 84-85 0.6622598506485353 1102 86-87 0.9458665134953002 1102 88-89 0.7309115126838428 1102 90-91 0.6830022076525424 1102 92-93 0.8045901004965046 1102 94-95 0.8881469210211925 1102 96-97 1.009868875342729 1102 98-99 1.052389910406145 1102 100-101 1.0932152170265077 1103 1 0.06717770212570429 1103 2 0.0682326886786413 1103 3 0.10189710534984187 1103 4 0.066740412294088 1103 5 0.08305166780307616 1103 6 0.025346238633723317 1103 7 0.07585416252235433 1103 8 0.1092119495476851 1103 9 5.518943020916822E-4 1103 10-11 0.06383395508642309 1103 12-13 0.11812641817986247 1103 14-15 0.0760199384774225 1103 16-17 0.10058838130984071 1103 18-19 0.09736222188631416 1103 20-21 0.12926113998383926 1103 22-23 0.16165691332904686 1103 24-25 0.11686837877709166 1103 26-27 0.14052604169113891 1103 28-29 0.1674587964773977 1103 30-31 0.27804739223605424 1103 32-33 0.17198690947191864 1103 34-35 0.18916483784339988 1103 36-37 0.12599671007031077 1103 38-39 0.13884303991694225 1103 40-41 0.34170886868231065 1103 42-43 0.3998624827053092 1103 44-45 0.3536493220071364 1103 46-47 0.2844045079372961 1103 48-49 0.20768681126965305 1103 50-51 0.13922490681832755 1103 52-53 0.14967737962779637 1103 54-55 0.21392390741687706 1103 56-57 0.13751026618451334 1103 58-59 0.1107261675892417 1103 60-61 0.09106630939740512 1103 62-63 0.03897196375880441 1103 64-65 0.04808145529839436 1103 66-67 0.13965069086879112 1103 68-69 0.15063128712765206 1103 70-71 0.17899175447713134 1103 72-73 0.09073096620440424 1103 74-75 0.1595956920650039 1103 76-77 -0.03814817435400997 1103 78-79 0.13820662210899215 1103 80-81 0.15481578237896443 1103 82-83 0.12255853792392557 1103 84-85 0.2792434715181642 1103 86-87 0.4841980437957396 1103 88-89 0.42636597186887215 1103 90-91 0.5009623131774106 1103 92-93 0.557339251173115 1103 94-95 0.5854167496381741 1103 96-97 0.5376109087842451 1103 98-99 0.5795957788037249 1103 100-101 0.8387294609863503 1104 1 0.10329602266613591 1104 2 0.07891614257817281 1104 3 0.12579682372192735 1104 4 0.06851853367020055 1104 5 0.01736977850309529 1104 6 0.05824148409178065 1104 7 0.09065711501374807 1104 8 0.061456689732118264 1104 9 0.05442565872862559 1104 10-11 0.1750420100130654 1104 12-13 0.09502924734399443 1104 14-15 0.17919147380975886 1104 16-17 0.2096455720253303 1104 18-19 0.19504293313486443 1104 20-21 0.19947208481789858 1104 22-23 0.10844948424222167 1104 24-25 0.12013655114074595 1104 26-27 0.11861980722208898 1104 28-29 0.17000541234060051 1104 30-31 0.05206767680910218 1104 32-33 0.08665733117751273 1104 34-35 0.15027441503337968 1104 36-37 0.18569446625047448 1104 38-39 0.1781816744817064 1104 40-41 0.3079227218308276 1104 42-43 0.17290351202774445 1104 44-45 0.30171105926485353 1104 46-47 0.31631037426526376 1104 48-49 0.22390618653425776 1104 50-51 0.24966170619985206 1104 52-53 0.3347731883416927 1104 54-55 0.3715619143011182 1104 56-57 0.34390579695190127 1104 58-59 0.4174594677898753 1104 60-61 0.3649752312962704 1104 62-63 0.2996112407297389 1104 64-65 0.318170913578804 1104 66-67 0.30610659677193297 1104 68-69 0.16444854189968083 1104 70-71 0.2811444593936585 1104 72-73 0.38444495740583307 1104 74-75 0.33180196150745545 1104 76-77 0.1818179566673983 1104 78-79 0.3668829708858432 1104 80-81 0.4191097798387915 1104 82-83 0.3886582673402401 1104 84-85 0.4544694181008957 1104 86-87 0.49842761656802637 1104 88-89 0.48531640167357537 1104 90-91 0.6801641833340568 1104 92-93 0.5402381780418892 1104 94-95 0.7623895256059221 1104 96-97 0.6492110339522092 1104 98-99 0.8044775134844535 1104 100-101 0.8445654173248194 1105 1 -0.00500016675622561 1105 2 -0.006055902580499151 1105 3 0.027734381678001796 1105 4 -0.04185274770159708 1105 5 -0.07159387365873471 1105 6 -0.0818289844601523 1105 7 -0.03261980857433855 1105 8 -0.039102709145659276 1105 9 -0.014379597768247265 1105 10-11 0.059693553645878694 1105 12-13 0.06576627248171008 1105 14-15 -0.0029272947475149635 1105 16-17 -0.005986787327039167 1105 18-19 0.07676822962331897 1105 20-21 0.09006019366508156 1105 22-23 0.04909943807873418 1105 24-25 -0.04498654983800776 1105 26-27 -0.030605456544151366 1105 28-29 0.0010378284785943492 1105 30-31 0.019703013379753997 1105 32-33 0.06604095033521418 1105 34-35 0.12513368433118188 1105 36-37 0.2008989700356807 1105 38-39 0.25836998328680494 1105 40-41 0.20375028682470742 1105 42-43 0.2383621667519762 1105 44-45 0.27657058027201487 1105 46-47 0.20838986106009116 1105 48-49 0.17167095709110214 1105 50-51 0.14652920840734396 1105 52-53 0.13366471066982655 1105 54-55 0.3217673846502649 1105 56-57 0.2127030490911821 1105 58-59 0.19110957567250608 1105 60-61 0.11360970802775228 1105 62-63 0.10892415976288561 1105 64-65 -0.1208711208771831 1105 66-67 -0.01872168710387001 1105 68-69 0.10403099634783786 1105 70-71 0.181382853326852 1105 72-73 0.09428133122040094 1105 74-75 0.1021706603412369 1105 76-77 -0.1989706034443195 1105 78-79 0.04335627610156223 1105 80-81 0.06446199170960298 1105 82-83 0.1331348830374388 1105 84-85 0.19479081571207146 1105 86-87 0.1973411886359422 1105 88-89 0.31476997086193137 1105 90-91 0.30242545966159895 1105 92-93 0.3991951045296709 1105 94-95 0.4976063832864348 1105 96-97 0.6187944751010512 1105 98-99 0.6948438663870142 1105 100-101 0.5348361710131151 1106 1 -0.0024849904917658705 1106 2 0.03952445065041843 1106 3 0.0053910570315736095 1106 4 0.04603729766622422 1106 5 0.01716893702563027 1106 6 0.046759447447691116 1106 7 0.1040930505472275 1106 8 0.0998752731071022 1106 9 0.1227313372271368 1106 10-11 0.10547112932628977 1106 12-13 0.1248807212924703 1106 14-15 0.08247855867438858 1106 16-17 0.016022245570077587 1106 18-19 -0.0025803105553521277 1106 20-21 0.07226242589158005 1106 22-23 0.10687490440482605 1106 24-25 0.15093722099229723 1106 26-27 0.12504735399981826 1106 28-29 0.05334655961750201 1106 30-31 0.09969925895178022 1106 32-33 0.22434643005718868 1106 34-35 0.09052686323027359 1106 36-37 0.10703064564341958 1106 38-39 0.0942357601871251 1106 40-41 0.2948105363162483 1106 42-43 0.20292609151623964 1106 44-45 0.15085086054454422 1106 46-47 0.23793455860231205 1106 48-49 0.2666687567203567 1106 50-51 0.2503684922629148 1106 52-53 0.16149407518629744 1106 54-55 0.20788918158581993 1106 56-57 0.2613668261473876 1106 58-59 0.19274666119067518 1106 60-61 0.2769045707793296 1106 62-63 0.1868839125854862 1106 64-65 0.12318908843157317 1106 66-67 0.20111271865805946 1106 68-69 0.10138171607966484 1106 70-71 0.1495232882982762 1106 72-73 0.09128547440474932 1106 74-75 0.1515117957519081 1106 76-77 -0.0398193486520384 1106 78-79 0.07248232224592854 1106 80-81 0.10417813904197715 1106 82-83 0.14153193594461655 1106 84-85 0.2874106294091767 1106 86-87 0.6150475647729827 1106 88-89 0.39465456395794973 1106 90-91 0.5602281825054156 1106 92-93 0.6019175126309051 1106 94-95 0.63864119174767 1106 96-97 0.6625449380503419 1106 98-99 0.7308201096449025 1106 100-101 1.2474450578966305 1107 1 -0.015436431707939846 1107 2 0.06082627395515061 1107 3 0.02759992556485713 1107 4 0.04273582609437909 1107 5 0.07433044322819171 1107 6 0.06083940400493759 1107 7 0.015262721443107807 1107 8 0.06042997271692485 1107 9 -0.027319692106075877 1107 10-11 0.007802283505974117 1107 12-13 0.08419412418658823 1107 14-15 0.08992557890744735 1107 16-17 0.042613158700469 1107 18-19 0.09555193867358724 1107 20-21 0.10064801868779227 1107 22-23 0.12729540762608593 1107 24-25 0.08898376339248415 1107 26-27 0.12015951797096136 1107 28-29 0.14802924229174863 1107 30-31 0.17483258536270796 1107 32-33 0.1468607145286498 1107 34-35 0.11097683343588471 1107 36-37 0.1519027823178476 1107 38-39 0.2062043371562865 1107 40-41 0.10306641357643542 1107 42-43 0.22779809722629096 1107 44-45 0.12512144744268028 1107 46-47 0.02558798570613874 1107 48-49 0.1891797086162086 1107 50-51 0.12689837833326578 1107 52-53 0.1666530191644071 1107 54-55 0.3096813004303769 1107 56-57 0.4253114216685887 1107 58-59 0.31113564970603846 1107 60-61 0.28653594815972383 1107 62-63 0.38406674025601717 1107 64-65 0.41249038647561065 1107 66-67 0.32312585391712645 1107 68-69 0.3808678188885679 1107 70-71 0.5257278097497746 1107 72-73 0.41564236916458697 1107 74-75 0.4353845035245527 1107 76-77 0.393818605478625 1107 78-79 0.7496594646593948 1107 80-81 0.6028033639031314 1107 82-83 0.46511415304051695 1107 84-85 0.5576857270945688 1107 86-87 0.7134849493021598 1107 88-89 1.0672170246101693 1107 90-91 1.0449574440627885 1107 92-93 1.0526216449311185 1107 94-95 1.0008266784627367 1107 96-97 1.0147558505633363 1107 98-99 1.129613647114276 1107 100-101 1.3572186953292409 1108 1 0.03147250623043618 1108 2 0.0527691659289502 1108 3 0.04692961595785761 1108 4 0.019246301336409033 1108 5 0.00706696964309117 1108 6 0.06912938262805568 1108 7 0.032759137167559516 1108 8 0.11237907457330465 1108 9 0.09237221759020997 1108 10-11 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sequence quality scores pass #Quality Count 3 10.0 4 24.0 5 89.0 6 171.0 7 339.0 8 901.0 9 1675.0 10 2697.0 11 4208.0 12 6173.0 13 8570.0 14 10611.0 15 13916.0 16 18556.0 17 25975.0 18 25454.0 19 17936.0 20 17006.0 21 18580.0 22 19924.0 23 21973.0 24 25341.0 25 30126.0 26 35706.0 27 44092.0 28 53060.0 29 64662.0 30 77946.0 31 95041.0 32 118598.0 33 152057.0 34 206383.0 35 295547.0 36 467603.0 37 690185.0 38 436264.0 39 21035.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.041818741800874 19.41523581494977 14.514043072916166 23.02890237033319 2 15.677904818133728 20.958422735975095 39.30377878467882 24.05989366121236 3 19.718243818422327 24.70339455969653 29.971595880907426 25.60676574097372 4 13.21914230258939 16.81331011341175 36.634808617258955 33.3327389667399 5 13.784120770008526 37.582823333775806 33.860800664633935 14.772255231581735 6 31.938685142222017 35.756136669975305 17.24376360851846 15.061414579284211 7 28.43103069110966 33.07006195281125 20.614515620944687 17.884391735134397 8 26.186999617624156 34.78877201880576 19.64589619585568 19.3783321677144 9 26.50482064327636 17.063109184482805 20.63191735398559 35.80015281825524 10-11 25.339498896129154 26.400080041367914 26.811001989807277 21.449419072695658 12-13 26.32345628136522 23.864875377835542 27.53290974807442 22.27875859272482 14-15 22.96981525395997 25.10008170980027 26.35911267350537 25.570990362734385 16-17 22.29192791165537 28.478280789897525 26.811277032922852 22.418514265524248 18-19 22.07290338622232 27.30082924456622 28.907063388520545 21.719203980690914 20-21 23.112473311288937 26.111515060258867 28.71782908262158 22.05818254583062 22-23 22.66613701999119 26.158370960040735 28.75152636643229 22.423965653535788 24-25 22.74858738779672 26.28782815661702 28.821284413848485 22.14230004173777 26-27 22.559679359035066 26.297370191987014 29.036145413768306 22.106805035209618 28-29 22.06670180033641 26.81735841032032 29.02239243120372 22.093547358139553 30-31 22.36390160723331 26.375364297191222 28.801964975957873 22.458769119617596 32-33 22.23989362158792 26.568698541886665 28.811161147972847 22.380246688552567 34-35 22.88144381136187 26.425418281984953 28.572317878126483 22.120820028526694 36-37 22.74358179570177 26.16904614025895 28.795464465109323 22.291907598929956 38-39 22.428236824876304 26.579714524194607 28.86720314124109 22.124845509687997 40-41 22.56884339139767 26.500206389829113 28.7007182366053 22.23023198216792 42-43 22.28774891378199 26.655917999724267 28.302379318970406 22.753953767523342 44-45 22.54870380475386 26.510686810533347 28.19219500839144 22.748414376321353 46-47 22.8167905330774 26.86566523477602 27.768750266229958 22.548793965916623 48-49 22.870778764270565 26.796151962670088 27.762652191012332 22.57041708204702 50-51 22.421106872188815 27.02917514813435 27.893372658615483 22.65634532106135 52-53 22.834606928861582 27.21239756256864 27.34226666323255 22.610728845337228 54-55 22.404615718883093 26.792262931931155 27.603705413424894 23.199415935760857 56-57 22.625802642553875 27.139158390111852 27.4362426257267 22.798796341607577 58-59 23.015342196612913 26.747858820421587 27.436222773482804 22.80057620948269 60-61 22.72432861525436 26.79818114365521 28.000620132205718 22.476870108884714 62-63 22.505027003582263 26.821852304909417 27.906271788566517 22.766848902941806 64-65 22.309134292163684 27.020596933310266 27.730952723737722 22.93931605078833 66-67 22.375239073925357 26.99656446806712 27.344592042140413 23.283604415867114 68-69 22.14773367059014 26.953709191954044 27.70593275887404 23.192624378581773 70-71 22.51264020456791 26.840193208894902 27.453447856844733 23.19371872969246 72-73 22.50829966906989 26.534720584962397 27.641612793939046 23.315366952028672 74-75 22.134855836382762 27.297656148359184 27.256116527551868 23.311371487706186 76-77 22.335409831947718 26.752789358561557 27.421085230529023 23.4907155789617 78-79 22.29315610905488 26.82686174898133 27.4811665907447 23.398815551219094 80-81 22.328214121614558 27.006911340320567 27.36660284368609 23.298271694378784 82-83 22.314820516943826 26.78290607999152 27.502469511052496 23.39980389201216 84-85 22.200547974154905 26.70549319737719 27.29228287617914 23.801675952288768 86-87 22.079093453409467 26.607232330657094 27.550240676670956 23.763433539262486 88-89 22.109993001681584 26.579978175860113 27.285100215256335 24.024928607201968 90-91 22.100404229902008 26.437020145855605 27.457531991381334 24.005043632861057 92-93 22.17107917821554 26.537609867013778 27.33693387407485 23.95437708069583 94-95 22.01783710638534 26.327612612047396 27.392644843524945 24.261905438042323 96-97 21.663605678710514 26.243348872717714 27.65105661870128 24.441988829870485 98-99 21.730323328822752 26.32606489030304 27.220256409748405 24.723355371125802 100-101 21.792734984525747 25.476160403907322 28.0743325515719 24.65677205999503 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 623.0 1 447.5 2 348.5 3 440.0 4 404.5 5 420.0 6 729.0 7 1424.0 8 2340.0 9 2847.0 10 2720.0 11 2598.0 12 2805.0 13 3112.5 14 3450.5 15 3771.5 16 3914.0 17 4101.5 18 4407.5 19 4742.5 20 5257.5 21 6009.0 22 7144.5 23 8508.0 24 10033.5 25 12380.0 26 15795.5 27 19619.0 28 22991.5 29 26619.5 30 30975.0 31 35981.5 32 40942.5 33 45817.5 34 52273.5 35 57872.5 36 63490.0 37 70573.5 38 75984.0 39 78934.5 40 81726.0 41 87214.0 42 92488.5 43 97649.5 44 103378.5 45 106776.5 46 109517.0 47 119430.0 48 142309.5 49 143417.5 50 124472.0 51 116202.5 52 110917.0 53 108710.5 54 106156.5 55 98750.5 56 88995.5 57 79230.5 58 71655.0 59 63707.5 60 54713.5 61 45121.5 62 36127.5 63 28551.0 64 22114.5 65 16826.5 66 11728.5 67 9068.5 68 6628.5 69 3886.5 70 2542.5 71 1704.5 72 1238.0 73 954.5 74 711.5 75 577.0 76 380.0 77 157.0 78 59.5 79 35.5 80 21.0 81 14.5 82 11.0 83 6.0 84 2.5 85 2.5 86 4.0 87 3.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39977757481259285 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011061822711011698 16-17 0.002195854359051576 18-19 4.953054945229119E-4 20-21 0.0 22-23 0.0 24-25 6.604073260305492E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012977003956500291 36-37 0.02306472586161693 38-39 0.010946251428956352 40-41 0.006075747399481052 42-43 0.00500258549468141 44-45 0.0017005488645286638 46-47 0.001221753553156516 48-49 0.002806731135629834 50-51 5.778564102767305E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.099378754828403E-4 60-61 0.001568467399322554 62-63 0.0013043044689103344 64-65 7.26448058633604E-4 66-67 0.0027076700367252514 68-69 0.002608608937820669 70-71 6.604073260305492E-5 72-73 0.0 74-75 0.0 76-77 0.09338159590071964 78-79 0.0034836486448111465 80-81 1.81612014658401E-4 82-83 1.651018315076373E-5 84-85 2.1463238095992845E-4 86-87 0.09239098491167382 88-89 0.44326539723170455 90-91 0.3948410300505145 92-93 0.0 94-95 9.245702564427688E-4 96-97 0.0 98-99 0.0 100-101 5.44836043975203E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3028434.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.465702349620564 #Duplication Level Percentage of deduplicated Percentage of total 1 71.59462164629234 33.98289000896613 2 14.760700492774632 14.012540321238765 3 5.441725090257641 7.74885910207894 4 2.5933628584656363 4.923831580979643 5 1.4732782457701696 3.4965093345949003 6 0.8920119773666579 2.5403985005989353 7 0.609819423312883 2.0261855063790595 8 0.43378243740017597 1.6471830446503732 9 0.32878808833968276 1.4045541783508924 >10 1.6752612766809913 14.291808737428893 >50 0.121290311436173 3.98794861460653 >100 0.069648936953046 5.8883416441069185 >500 0.0036901023458311122 1.223065747986123 >1k 0.0017406143139790885 1.6860322712922526 >5k 1.3924914511832709E-4 0.44978045419827434 >10k+ 1.3924914511832709E-4 0.6900709525432892 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10624 0.35080837158742767 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10257 0.3386898971547671 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8463 0.27945136000982684 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5147 0.1699558253539618 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4900 0.16179979487748453 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3851 0.12716143062718221 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3580 0.1182129113594683 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.302036630152745E-5 0.0 5 0.0 0.0 0.0 3.302036630152745E-5 0.0 6 3.302036630152745E-5 0.0 0.0 3.302036630152745E-5 0.0 7 3.302036630152745E-5 0.0 0.0 3.302036630152745E-5 0.0 8 3.302036630152745E-5 0.0 0.0 3.302036630152745E-5 0.0 9 3.302036630152745E-5 0.0 0.0 6.60407326030549E-5 0.0 10-11 9.906109890458237E-5 0.0 0.0 1.320814652061098E-4 0.0 12-13 1.6510183150763728E-4 0.0 0.0 1.320814652061098E-4 0.0 14-15 1.6510183150763728E-4 0.0 0.0 1.320814652061098E-4 0.0 16-17 1.6510183150763728E-4 0.0 0.0 2.1463238095992845E-4 0.0 18-19 1.6510183150763728E-4 0.0 0.0 3.63224029316802E-4 0.0 20-21 1.6510183150763728E-4 0.0 0.0 4.127545787690932E-4 0.0 22-23 2.641629304122196E-4 0.0 0.0 7.099378754828403E-4 0.0 24-25 2.971832967137471E-4 0.0 0.0 8.750397069904776E-4 0.0 26-27 2.971832967137471E-4 0.0 0.0 0.0014694063004179717 0.0 28-29 2.971832967137471E-4 0.0 0.0 0.003962443956183294 0.0 30-31 2.971832967137471E-4 0.0 0.0 0.011226924542519334 0.0 32-33 2.971832967137471E-4 0.0 0.0 0.025838436630945233 0.0 34-35 3.63224029316802E-4 0.0 0.0 0.04936544762078354 0.0 36-37 4.4577494507062063E-4 0.0 0.0 0.08738839941699242 0.0 38-39 4.622851282213844E-4 0.0 0.0 0.14476128586589637 0.0 40-41 4.622851282213844E-4 0.0 0.0 0.20850710301099512 0.0 42-43 4.622851282213844E-4 0.0 0.0 0.2754724058704928 0.0 44-45 4.622851282213844E-4 0.0 0.0 0.3588653409650004 0.0 46-47 4.787953113721481E-4 0.0 0.0 0.46033692660959424 0.0 48-49 4.953054945229118E-4 0.0 0.0 0.5688253400932628 0.0 50-51 4.953054945229118E-4 0.0 0.0 0.6932460803174182 0.0 52-53 4.953054945229118E-4 0.0 0.0 0.8141336413473101 0.0 54-55 4.953054945229118E-4 0.0 0.0 0.9504582236231662 0.0 56-57 4.953054945229118E-4 0.0 0.0 1.117557787292046 0.0 58-59 4.953054945229118E-4 0.0 0.0 1.3034954699359471 0.0 60-61 4.953054945229118E-4 0.0 0.0 1.513835203276677 0.0 62-63 4.953054945229118E-4 0.0 0.0 1.723184325628361 0.0 64-65 4.953054945229118E-4 0.0 0.0 1.9360666271743088 0.0 66-67 4.953054945229118E-4 0.0 0.0 2.1921725882089556 0.0 68-69 4.953054945229118E-4 0.0 0.0 2.4898016598677732 0.0 70-71 4.953054945229118E-4 0.0 0.0 2.8223167485241545 0.0 72-73 4.953054945229118E-4 0.0 0.0 3.154088878938752 0.0 74-75 4.953054945229118E-4 0.0 0.0 3.5005550723575287 0.0 76-77 4.953054945229118E-4 0.0 0.0 3.8684184631396956 0.0 78-79 4.953054945229118E-4 0.0 0.0 4.2923339257187045 0.0 80-81 4.953054945229118E-4 0.0 0.0 4.76696867093686 0.0 82-83 4.953054945229118E-4 0.0 0.0 5.249462263334779 0.0 84-85 4.953054945229118E-4 0.0 0.0 5.742522372949187 0.0 86-87 4.953054945229118E-4 0.0 0.0 6.2587627797072685 0.0 88-89 5.118156776736755E-4 0.0 0.0 6.819465109690355 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 505 0.0 25.370476 3 GTACATG 13955 0.0 24.168001 1 TACATGG 14315 0.0 23.07141 2 TACCGTA 150 3.059018E-5 22.144354 7 ACATGGG 14325 0.0 21.895912 3 GAGTACT 9955 0.0 21.547476 12-13 CTTTGCG 615 0.0 20.832666 2 GTACTTT 10450 0.0 20.254328 14-15 GTATAGG 750 0.0 19.689638 1 CATGGGG 6160 0.0 19.48929 4 GTATCAA 26990 0.0 18.920353 1 AGTACTT 10290 0.0 18.908964 12-13 TGTACCG 340 9.0403773E-10 18.143482 5 GGTATCA 21910 0.0 18.045671 1 CATGGGA 8550 0.0 17.981882 4 TCTATAC 870 0.0 17.453678 3 AGTACAT 10855 0.0 17.310978 2 ACCGTAT 195 2.2944383E-4 17.034119 8 AGAGTAC 20315 0.0 16.81957 10-11 ACTTTTT 12930 0.0 16.73543 16-17 >>END_MODULE