FastQCFastQC Report
Fri 27 May 2016
SRR939004_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939004_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043888
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104370.3428838380387189No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT100400.3298413082215903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81600.2680781947298981No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52900.1737908884952403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49310.16199676203592248No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42250.13880274175659552No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38740.12727143705681682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG139250.024.5520611
TACATGG140450.023.5889842
GAGTACT96100.022.90048412-13
TTTGCGT5450.022.5796363
ACATGGG143350.022.154653
GTACTTT104450.020.9341714-15
CATGGGG62400.020.6315334
GTATCAA271800.020.0455741
AGTACTT101500.019.65380512-13
CAACGCA302000.017.8507655
TCAACGC303850.017.7891044
ATCAACG303400.017.7839973
TATCAAC305700.017.7273162
AACGCAG306850.017.5968656
CATGGGA84050.017.569694
ACTTTTT126000.017.54316116-17
ACTATCG1350.0096022617.5289088
AGAGTAC201150.017.43479310-11
GGTATCA215800.017.3589551
CTTTGCG7500.017.038662
Produced by FastQC (version 0.11.5)