##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939004_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3043888 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8643360071067 33.0 31.0 34.0 30.0 34.0 2 31.92689875580179 33.0 31.0 34.0 30.0 34.0 3 31.98062740810437 34.0 31.0 34.0 30.0 34.0 4 35.508960907891485 37.0 35.0 37.0 33.0 37.0 5 35.5449927855427 37.0 35.0 37.0 33.0 37.0 6 35.62469906908533 37.0 35.0 37.0 33.0 37.0 7 35.539491597588345 37.0 35.0 37.0 33.0 37.0 8 35.5222971410249 37.0 35.0 37.0 33.0 37.0 9 37.14076930557235 39.0 37.0 39.0 33.0 39.0 10-11 37.03258398469326 39.0 37.0 39.0 33.0 39.0 12-13 36.91359701802432 39.0 37.0 39.0 33.0 39.0 14-15 38.14161723427406 40.0 38.0 41.0 33.0 41.0 16-17 38.075066001114365 40.0 38.0 41.0 33.0 41.0 18-19 38.01390146418002 40.0 38.0 41.0 33.0 41.0 20-21 37.90122419090321 40.0 38.0 41.0 32.5 41.0 22-23 37.976403533901376 40.0 38.0 41.0 33.0 41.0 24-25 37.909848029888096 40.0 38.0 41.0 33.0 41.0 26-27 37.83184860941007 40.0 38.0 41.0 32.0 41.0 28-29 37.70894658410559 40.0 38.0 41.0 32.0 41.0 30-31 37.5303973076539 40.0 37.0 41.0 32.0 41.0 32-33 37.34699026376792 40.0 37.0 41.0 31.5 41.0 34-35 37.03248690490583 40.0 36.5 41.0 30.5 41.0 36-37 36.76551995342798 40.0 36.0 41.0 30.0 41.0 38-39 36.57097468763634 39.5 36.0 41.0 30.0 41.0 40-41 36.423170957669925 39.0 35.5 41.0 30.0 41.0 42-43 36.208934428599214 39.0 35.0 41.0 29.5 41.0 44-45 36.14146956129792 39.0 35.0 41.0 29.0 41.0 46-47 35.914361665080975 39.0 35.0 41.0 28.0 41.0 48-49 35.7284430307554 39.0 35.0 41.0 27.0 41.0 50-51 34.54803051229217 38.0 34.0 40.0 25.0 40.5 52-53 34.68292837975642 38.0 34.5 39.5 25.5 40.5 54-55 35.174067015606354 39.0 35.0 40.5 26.0 41.0 56-57 35.06740918194099 39.0 35.0 41.0 26.0 41.0 58-59 34.86536544708609 38.0 34.5 40.5 26.0 41.0 60-61 34.59218966006634 38.0 34.0 40.0 25.5 41.0 62-63 34.238540971284095 37.5 34.0 40.0 24.5 41.0 64-65 33.8329692156873 37.0 34.0 40.0 23.0 41.0 66-67 33.450857423137776 36.5 33.0 39.0 22.0 41.0 68-69 33.0636370326372 36.0 33.0 39.0 22.0 41.0 70-71 32.64339226673255 35.5 32.5 39.0 20.5 40.0 72-73 32.21015441435427 35.0 32.0 38.0 19.5 40.0 74-75 31.757125262164706 35.0 31.5 37.0 18.0 39.0 76-77 31.34803957964288 35.0 31.0 36.5 17.0 39.0 78-79 30.92747909909957 35.0 31.0 36.0 12.0 38.5 80-81 30.502746487387185 34.5 31.0 36.0 7.5 37.5 82-83 30.139068848788128 34.0 30.5 35.0 7.0 37.0 84-85 29.803379427889595 34.0 30.0 35.0 2.0 36.5 86-87 29.43357672818448 34.0 30.0 35.0 2.0 36.0 88-89 29.03483735275411 34.0 29.0 35.0 2.0 36.0 90-91 28.629714365311735 34.0 29.0 35.0 2.0 35.5 92-93 28.231190832251386 34.0 29.0 35.0 2.0 35.0 94-95 27.770357516439503 34.0 28.0 35.0 2.0 35.0 96-97 27.20549162781285 34.0 26.5 35.0 2.0 35.0 98-99 26.52752729403973 33.0 25.0 35.0 2.0 35.0 100-101 23.919933979174004 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22497313551732745 1101 2 0.22606354227789893 1101 3 0.21609800414567104 1101 4 0.06153886629100924 1101 5 0.017031229979153295 1101 6 0.11443499891913689 1101 7 0.15021528766767744 1101 8 0.18456355910542044 1101 9 0.20369414779984396 1101 10-11 0.2536126787210833 1101 12-13 0.3610649930636427 1101 14-15 0.14944805850965537 1101 16-17 0.27598860628410904 1101 18-19 0.35778080652497835 1101 20-21 0.4379508771173022 1101 22-23 0.41675652248208905 1101 24-25 0.49366639999384887 1101 26-27 0.4630049752976859 1101 28-29 0.38995372026062824 1101 30-31 0.36925947724453323 1101 32-33 0.3917117007517774 1101 34-35 0.2058350863874452 1101 36-37 0.1781210205847188 1101 38-39 0.11714558025234822 1101 40-41 0.3368574302822154 1101 42-43 0.18224067375972197 1101 44-45 0.31058376850875646 1101 46-47 0.28633811771022977 1101 48-49 0.26902259850122334 1101 50-51 0.2112390346682531 1101 52-53 0.1591864607230562 1101 54-55 -0.0372096879334336 1101 56-57 -0.030342739885782066 1101 58-59 -0.05869442171768924 1101 60-61 0.004210015336269635 1101 62-63 -0.030332117515932566 1101 64-65 -0.03901757592438315 1101 66-67 -0.0834433096081284 1101 68-69 -0.07019659251685795 1101 70-71 -0.09481952168257379 1101 72-73 -0.22208160733780602 1101 74-75 -0.2932248947711855 1101 76-77 -0.25669520414114544 1101 78-79 -0.2245162526996225 1101 80-81 -0.3042449388722126 1101 82-83 -0.3973659939512224 1101 84-85 -0.4411891174104703 1101 86-87 -0.34930129191020853 1101 88-89 -0.3957210348942013 1101 90-91 -0.46565694139590263 1101 92-93 -0.3535931678960793 1101 94-95 -0.5535735933340611 1101 96-97 -0.6617945422246372 1101 98-99 -0.7760051785292319 1101 100-101 -0.48785684728264656 1102 1 0.19484868184210669 1102 2 0.12516515949630502 1102 3 0.13578769490401044 1102 4 0.14719094596465254 1102 5 0.18987140713234396 1102 6 0.21927241978089995 1102 7 0.20668949919686952 1102 8 0.1495133667796864 1102 9 0.2823642695360462 1102 10-11 0.2562026868411422 1102 12-13 0.30639337024853575 1102 14-15 0.311827592075268 1102 16-17 0.28896161597965175 1102 18-19 0.39113387386971255 1102 20-21 0.41297538287737723 1102 22-23 0.38591032160279326 1102 24-25 0.3323874419496491 1102 26-27 0.36167406180705797 1102 28-29 0.37865713100951837 1102 30-31 0.3506354231021689 1102 32-33 0.47796324146045066 1102 34-35 0.3961634797477558 1102 36-37 0.3960983814680219 1102 38-39 0.30677584252059376 1102 40-41 0.35989122720305744 1102 42-43 0.31676775302897653 1102 44-45 0.4468726744357454 1102 46-47 0.4186388122827296 1102 48-49 0.4584742841416585 1102 50-51 0.39109934374816646 1102 52-53 0.4266010787728831 1102 54-55 0.4823260128342497 1102 56-57 0.4397582917824536 1102 58-59 0.4286864867585578 1102 60-61 0.27507485029846634 1102 62-63 0.33979382429784977 1102 64-65 0.3514806593373905 1102 66-67 0.2794829531259637 1102 68-69 0.2047256845939458 1102 70-71 0.26409717814370737 1102 72-73 0.26258247773196075 1102 74-75 0.24508626206244344 1102 76-77 0.15747478657416636 1102 78-79 0.0063545348168361215 1102 80-81 -0.09987239955785654 1102 82-83 -0.14335335771375313 1102 84-85 -0.22833289424144354 1102 86-87 -0.18337707698417915 1102 88-89 -0.13175275165614053 1102 90-91 -0.1802520221274193 1102 92-93 -0.1574277127683068 1102 94-95 -0.25368520372644454 1102 96-97 -0.4325490608279914 1102 98-99 -0.4408999897782664 1102 100-101 -0.10833751603917463 1103 1 0.02780267223800692 1103 2 0.19121586965419368 1103 3 0.22805620373102897 1103 4 0.2534532761960051 1103 5 0.26752886934077935 1103 6 0.2013659106874499 1103 7 0.1889523622845175 1103 8 0.24436876607302338 1103 9 0.2680488312934557 1103 10-11 0.3152466538294263 1103 12-13 0.31092645491559523 1103 14-15 0.25207156413836884 1103 16-17 0.28790256408326087 1103 18-19 0.26863877424461435 1103 20-21 0.2780000274812551 1103 22-23 0.3216583186340216 1103 24-25 0.36783856189941133 1103 26-27 0.21837631566233284 1103 28-29 0.22272751878934827 1103 30-31 0.18918313594337377 1103 32-33 0.2616330989213935 1103 34-35 0.2524394485629813 1103 36-37 0.3160103967432306 1103 38-39 0.3159726885187766 1103 40-41 0.3071673165452964 1103 42-43 0.20721693820289033 1103 44-45 0.28632021663606366 1103 46-47 0.21815797290719274 1103 48-49 0.20612337289395555 1103 50-51 0.12140237405120047 1103 52-53 0.2412888820727872 1103 54-55 0.2983516660589842 1103 56-57 0.41478065271648035 1103 58-59 0.31671973952909127 1103 60-61 0.2683037691710979 1103 62-63 0.26451613638671034 1103 64-65 0.06925014823938369 1103 66-67 0.07992287674989029 1103 68-69 0.04048763738541794 1103 70-71 -0.01211935181994761 1103 72-73 0.037502390698762156 1103 74-75 -0.0416202419561813 1103 76-77 0.0654298870873582 1103 78-79 0.1603598380057285 1103 80-81 0.08158832449235476 1103 82-83 0.20199556800988105 1103 84-85 0.12986706642868384 1103 86-87 0.29089769924789266 1103 88-89 0.17321993945848035 1103 90-91 0.031039456612248273 1103 92-93 0.09509669395329468 1103 94-95 0.003688195325832311 1103 96-97 -0.2085251711038687 1103 98-99 -0.2647535557921863 1103 100-101 0.07662959277135073 1104 1 0.08205505448481532 1104 2 0.058978914341430766 1104 3 0.1014114496616898 1104 4 0.15442712322845153 1104 5 0.09979824465718679 1104 6 0.18412545152044402 1104 7 0.0897850576088075 1104 8 0.08088266801996724 1104 9 0.22846849577135941 1104 10-11 0.07661360587525934 1104 12-13 0.13194770996875604 1104 14-15 0.1712664507556454 1104 16-17 0.07159505286718826 1104 18-19 0.2267435257702246 1104 20-21 0.2747653839641302 1104 22-23 0.21542270501063854 1104 24-25 0.2857696918362933 1104 26-27 0.2800576170511633 1104 28-29 0.2444631361534988 1104 30-31 0.39149979458816375 1104 32-33 0.24828578342238217 1104 34-35 0.2519194815815027 1104 36-37 0.20381985191260554 1104 38-39 0.12172424654934133 1104 40-41 0.17428819577579446 1104 42-43 0.04949012910433481 1104 44-45 0.0954733812025026 1104 46-47 0.16438465856033702 1104 48-49 0.25092719446422507 1104 50-51 0.13058247215911933 1104 52-53 0.2221065893800116 1104 54-55 0.35496597026335763 1104 56-57 0.2805662018739312 1104 58-59 0.35670320532722855 1104 60-61 0.17018275776673875 1104 62-63 0.09252789237879 1104 64-65 0.0666299884835837 1104 66-67 0.20033600735013835 1104 68-69 0.09933418353280388 1104 70-71 0.08426157823514302 1104 72-73 0.0312385966106703 1104 74-75 0.09795111571875381 1104 76-77 0.06341997155393386 1104 78-79 0.09885559971371194 1104 80-81 -0.10181163610486976 1104 82-83 -0.13290531406360273 1104 84-85 0.02972965549017559 1104 86-87 0.16188528737419006 1104 88-89 0.10936076197890898 1104 90-91 0.05161368969212532 1104 92-93 0.19436524879422734 1104 94-95 0.2229261007940906 1104 96-97 0.053214735676153424 1104 98-99 6.979426354440932E-4 1104 100-101 0.5995896887345573 1105 1 -0.0013512608687626937 1105 2 0.04191128290147006 1105 3 0.05483158676986477 1105 4 0.09541616887227633 1105 5 0.15120890761127015 1105 6 0.10299957026703055 1105 7 0.20709818598041352 1105 8 0.1757503284853712 1105 9 0.17417429586350153 1105 10-11 0.09107863033290187 1105 12-13 0.23378906845524483 1105 14-15 0.2803216725026374 1105 16-17 0.1812190083755283 1105 18-19 0.12288993164781203 1105 20-21 0.19449197574323307 1105 22-23 0.10013316901849123 1105 24-25 0.07553473157174295 1105 26-27 0.08211077282718549 1105 28-29 0.19264972721055784 1105 30-31 0.12274072563073446 1105 32-33 0.0013102037682202194 1105 34-35 0.10647187406482317 1105 36-37 0.18153325887110583 1105 38-39 0.17854215395816908 1105 40-41 0.2653209230415712 1105 42-43 0.1141023489125601 1105 44-45 0.09308876392356069 1105 46-47 0.2200208669190431 1105 48-49 0.09652521308306916 1105 50-51 0.05036859718323683 1105 52-53 0.10885038865782093 1105 54-55 0.0413872738884038 1105 56-57 0.03112076959204302 1105 58-59 0.1236747826258906 1105 60-61 0.05942360608439401 1105 62-63 0.06826068326623869 1105 64-65 0.12411561263945003 1105 66-67 0.0821618585022037 1105 68-69 -0.05321515434514623 1105 70-71 -0.22571685112483664 1105 72-73 0.06337704511692976 1105 74-75 -0.0565722035011369 1105 76-77 -0.019489874790519224 1105 78-79 -0.013460023555129652 1105 80-81 -0.012292463889536975 1105 82-83 -0.07435024330152729 1105 84-85 0.005872365046602823 1105 86-87 0.05256473623501279 1105 88-89 0.009470790891985104 1105 90-91 0.007660701350360455 1105 92-93 0.1121929930556611 1105 94-95 -0.023072739144442522 1105 96-97 -0.14207492942424338 1105 98-99 -0.03193332876753274 1105 100-101 0.2959923744729025 1106 1 0.16600479785116207 1106 2 0.20782643039287052 1106 3 0.17751398001264818 1106 4 0.06222864409058815 1106 5 0.16241922232721606 1106 6 0.1852062011558573 1106 7 0.13774287877966174 1106 8 0.1332477696317369 1106 9 0.16208797720093315 1106 10-11 0.179621593715936 1106 12-13 0.22949370989299922 1106 14-15 0.10883854018915429 1106 16-17 0.19309877452857904 1106 18-19 0.09652301970003663 1106 20-21 0.2792918664732724 1106 22-23 0.2379400585590048 1106 24-25 0.21486955641706373 1106 26-27 0.27698937712236926 1106 28-29 0.16327587408414246 1106 30-31 0.07559868850660934 1106 32-33 0.12052448585993858 1106 34-35 0.20535254849565376 1106 36-37 0.05759691208420037 1106 38-39 0.10986275296044568 1106 40-41 0.05489803213633593 1106 42-43 0.16812873968408582 1106 44-45 0.3010197693916652 1106 46-47 0.2127651913355777 1106 48-49 0.13405270347007558 1106 50-51 0.11701048215930143 1106 52-53 0.09195734412914902 1106 54-55 -0.07662849648790626 1106 56-57 0.13374405562116465 1106 58-59 0.28411778704279556 1106 60-61 0.21685563509395678 1106 62-63 0.12444267769669892 1106 64-65 -0.053620631793315 1106 66-67 0.09242948923426297 1106 68-69 0.14365261285911402 1106 70-71 0.15929247370664967 1106 72-73 0.14831680831922966 1106 74-75 0.11045667927109548 1106 76-77 0.10915343714987458 1106 78-79 0.17963222427099623 1106 80-81 0.1969328950415523 1106 82-83 0.1973037097815471 1106 84-85 0.1700787861555284 1106 86-87 0.24671608435898307 1106 88-89 0.398372627733945 1106 90-91 0.32417389443084943 1106 92-93 0.3332483242312563 1106 94-95 0.5344218204099924 1106 96-97 0.5019850722355521 1106 98-99 0.26505131580539043 1106 100-101 0.6343938516921916 1107 1 0.05164585378667752 1107 2 0.07370781821420991 1107 3 0.08108886387044123 1107 4 0.15319262440214487 1107 5 0.06022099143383741 1107 6 0.02259095063644878 1107 7 0.006547884709377172 1107 8 0.0877429857320351 1107 9 0.04164093002178504 1107 10-11 0.08028461858508251 1107 12-13 0.1684706218924248 1107 14-15 0.12950076254018228 1107 16-17 0.23310140500465337 1107 18-19 0.18874271795322528 1107 20-21 0.12017108080802785 1107 22-23 0.0694882163340651 1107 24-25 0.13509864190307752 1107 26-27 0.24843310722661016 1107 28-29 0.182044822428729 1107 30-31 0.2546018049519887 1107 32-33 0.1683875027039008 1107 34-35 0.07056907234381526 1107 36-37 -0.034563154613977076 1107 38-39 0.13505357393648154 1107 40-41 -0.03346718271560434 1107 42-43 -2.1281202929657184E-4 1107 44-45 0.2655830769255303 1107 46-47 0.18730513667517812 1107 48-49 0.03633837540067475 1107 50-51 0.0025637069446275973 1107 52-53 -0.04316887413794035 1107 54-55 0.021174665879343024 1107 56-57 0.043367696913392706 1107 58-59 0.04266850618819973 1107 60-61 -0.009025630120447659 1107 62-63 -0.004307032505089126 1107 64-65 0.12655104020860364 1107 66-67 0.023090864482824713 1107 68-69 0.08638096270351525 1107 70-71 0.1022648113527751 1107 72-73 0.03728653109380531 1107 74-75 -0.06555923065070601 1107 76-77 0.06885493384617547 1107 78-79 0.13948603221949085 1107 80-81 0.033677413396926426 1107 82-83 -0.02759692287023796 1107 84-85 0.11373025476369492 1107 86-87 0.13888880186368624 1107 88-89 0.32383663925454087 1107 90-91 0.31458575341950024 1107 92-93 0.18854059687341618 1107 94-95 0.29211161594620805 1107 96-97 0.27992942206212135 1107 98-99 0.3946694174933576 1107 100-101 0.7111090112595413 1108 1 0.19165342816397768 1108 2 0.1752436440787406 1108 3 0.18404108038303235 1108 4 0.16438173001233025 1108 5 0.1928631329950079 1108 6 0.2252172943385915 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-1.6541930899427228 2316 56-57 -1.370210445796431 2316 58-59 -1.3498410597850565 2316 60-61 -1.2736570342701867 2316 62-63 -1.4495825434918075 2316 64-65 -1.8840750121093208 2316 66-67 -1.745815552723876 2316 68-69 -1.8222808845550134 2316 70-71 -1.7250750019661787 2316 72-73 -1.5634754596999763 2316 74-75 -1.7586658862306237 2316 76-77 -1.852649605349665 2316 78-79 -1.5445364488829192 2316 80-81 -1.599335572952004 2316 82-83 -1.6873045443608383 2316 84-85 -1.7888532580394312 2316 86-87 -1.6446955862731691 2316 88-89 -1.8207060384388178 2316 90-91 -1.9861805592584716 2316 92-93 -2.0378676982223993 2316 94-95 -2.0653699916973167 2316 96-97 -2.2543175535320152 2316 98-99 -2.656250900353836 2316 100-101 -3.312251990248857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9136.0 3 2340.0 4 2265.0 5 2690.0 6 3088.0 7 3790.0 8 4710.0 9 5851.0 10 7141.0 11 9260.0 12 12371.0 13 18087.0 14 17758.0 15 16014.0 16 20681.0 17 27038.0 18 23433.0 19 16767.0 20 17075.0 21 17946.0 22 19749.0 23 22150.0 24 25175.0 25 28893.0 26 33422.0 27 39467.0 28 46686.0 29 55861.0 30 67619.0 31 82475.0 32 102917.0 33 133442.0 34 181154.0 35 260572.0 36 402535.0 37 626346.0 38 583009.0 39 94954.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.12163956485096 18.837217117865716 14.009728845658362 23.03141447162497 2 15.397191321763623 20.500773210198417 40.025259624683144 24.076775843354813 3 19.714687169011828 24.638821104192157 29.800765056396028 25.845726670399987 4 12.893287158084451 16.247373042731837 37.115790027593334 33.74354977159038 5 13.838180558236918 37.3821137516169 33.915423492626466 14.864282197519715 6 31.99797191923583 35.39615802326572 17.546581247026353 15.059288810472104 7 28.484802048294473 32.972713631740405 20.72405210312897 17.818432216836147 8 26.18176305845919 34.46208674258405 19.882062915781162 19.4740872831756 9 26.69966957628947 17.07542131653855 20.75348970582819 35.471419401343795 10-11 25.340410269613777 26.23495777003837 26.94541351427825 21.4792184460696 12-13 26.454879000203057 23.79103375761567 27.539466642332705 22.21462059984857 14-15 23.155508710079488 24.93527869952281 26.440476464533187 25.46873612586451 16-17 22.365803616363653 28.374431762785328 26.80447016837858 22.45529445247244 18-19 22.140042285578605 27.21580805806983 28.926321868300388 21.717827788051185 20-21 23.201121331074184 26.064517974973956 28.666459256374903 22.067901437576957 22-23 22.720605467063667 26.10555381504606 28.727418945021004 22.446421772869265 24-25 22.83120006052771 26.17244476024119 28.75883655215745 22.237518627073648 26-27 22.602243238268773 26.186832131161886 29.01917909341804 22.1917455371513 28-29 22.119777696164252 26.71876189835542 28.979332243118467 22.182128162361863 30-31 22.34476180295449 26.322428388762575 28.808311713941524 22.524498094341407 32-33 22.239644607460228 26.505170748328627 28.80877917189749 22.446405472313646 34-35 22.904616592225892 26.36446763372336 28.550396142547 22.180519631503742 36-37 22.834341463092205 26.077828553771003 28.736862021585903 22.350967961550886 38-39 22.485737840610764 26.453352454181832 28.80995848154469 22.25095122366271 40-41 22.61902166660721 26.44240360744584 28.67715645433418 22.26141827161277 42-43 22.31725206577439 26.586128172173744 28.270960878313417 22.82565888373845 44-45 22.562405299431425 26.455236889806127 28.176131077979566 22.80622673278288 46-47 22.74657441906879 26.8229562495787 27.811639639592013 22.618829691760496 48-49 22.866759626885578 26.721364247949296 27.766852730323592 22.645023394841534 50-51 22.43555986203904 26.975339965898073 27.958608407303743 22.630491764759146 52-53 22.842851057121546 27.13654289709437 27.44178337642371 22.57882266936038 54-55 22.428555850026765 26.74378911748961 27.698272386604717 23.12938264587891 56-57 22.68223803063717 27.077813928235113 27.47812651397361 22.761821527154112 58-59 23.016340609315392 26.67036981796253 27.5272149411749 22.78607463154718 60-61 22.753243022837353 26.66438304152456 28.161489165711945 22.42088476992614 62-63 22.536468252761964 26.71814141104416 28.062811171305956 22.682579164887915 64-65 22.37868609678934 26.92017648543311 27.874825374648125 22.826312043129427 66-67 22.422665289498664 26.921419606991424 27.45303091834726 23.20288418516265 68-69 22.206214828407752 26.887931236820208 27.827173700682085 23.07868023408995 70-71 22.591987898577866 26.728067412008667 27.62142660455079 23.058518084862676 72-73 22.581422994993815 26.417844364560434 27.854302225670114 23.146430414775637 74-75 22.210499281584262 27.209843171513782 27.49312737096199 23.086530175939966 76-77 22.478640119116644 26.682984452938037 27.491790571628698 23.34658485631662 78-79 22.44485399189268 26.758191490451317 27.673048756594344 23.123905761061664 80-81 22.509420030097843 26.98012253885711 27.549933180145995 22.960524250899052 82-83 22.523534795811486 26.73237244619792 27.673681671441365 23.070411086549232 84-85 22.419720611452636 26.67122152559134 27.537030259519558 23.372027603436468 86-87 22.349940869500855 26.571534686753743 27.764685395675393 23.313839048070008 88-89 22.474068414375285 26.54028843239942 27.511175854931835 23.474467298293458 90-91 22.427386869275296 26.44040934875208 27.699028684985727 23.4331750969869 92-93 22.603754120060245 26.473714333785246 27.634479278759933 23.288052267394573 94-95 22.499809523935447 26.285366364389557 27.641489835189038 23.573334276485966 96-97 22.204006108935186 26.17897871177226 27.92576992597188 23.691245253320673 98-99 22.346150349968184 26.256108948773253 27.497979522522748 23.89976117873582 100-101 22.475003007306103 25.685864075051786 28.035792891124945 23.80334002651717 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3183.0 1 1837.5 2 540.0 3 609.0 4 563.5 5 595.0 6 919.0 7 1698.5 8 2834.5 9 3460.0 10 3356.5 11 3346.0 12 3740.5 13 4108.5 14 4422.5 15 4821.5 16 5141.5 17 5306.5 18 5389.0 19 5554.0 20 5969.0 21 6598.5 22 7514.5 23 8858.5 24 10322.5 25 12361.0 26 15802.5 27 19531.5 28 22700.5 29 26423.0 30 30906.5 31 36042.5 32 40944.0 33 45895.0 34 52516.0 35 58229.5 36 64265.5 37 71412.5 38 76265.5 39 78954.5 40 81896.0 41 87349.0 42 92724.0 43 97935.0 44 103526.0 45 107140.0 46 110184.5 47 119843.5 48 142717.5 49 143841.5 50 123914.5 51 114482.5 52 109156.0 53 107795.5 54 105131.0 55 97360.5 56 87996.0 57 78651.5 58 70985.5 59 62857.5 60 54111.0 61 44921.0 62 36410.0 63 29074.0 64 22266.0 65 17207.0 66 12467.5 67 9439.0 68 6963.5 69 4309.5 70 2888.5 71 1999.5 72 1428.5 73 1054.0 74 766.5 75 604.0 76 406.5 77 214.0 78 125.5 79 78.5 80 58.5 81 43.5 82 33.5 83 30.5 84 24.0 85 20.5 86 21.5 87 19.0 88 13.0 89 9.5 90 6.5 91 8.0 92 6.5 93 6.0 94 5.5 95 2.5 96 2.5 97 3.5 98 2.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28877540829360343 2 0.08774961496612227 3 0.09215187943840247 4 0.10834827037000047 5 0.11008946452694711 6 0.08505569193084633 7 0.09589708951183487 8 0.09116629784013078 9 0.09671840751039461 10-11 0.09006573172206073 12-13 0.09430373259462899 14-15 0.10374888957806595 16-17 0.09180692587900738 18-19 0.10299327701939098 20-21 0.10565434733472454 22-23 0.11795769095314938 24-25 0.13003106553197752 26-27 0.13277426764716704 28-29 0.12531670022024463 30-31 0.11828621815257329 32-33 0.1123234494830296 34-35 0.3888283668781506 36-37 0.5936650757189489 38-39 0.5756782115504907 40-41 0.5378647308968004 42-43 0.5643440231703664 44-45 0.5881622451285987 46-47 0.5762367077895113 48-49 0.5986094100702786 50-51 0.5900184238053436 52-53 0.5938786183985745 54-55 0.5830700735375284 56-57 0.5545046335476206 58-59 0.5498395473158013 60-61 0.49535331129134846 62-63 0.5722943813964246 64-65 0.6072003963352134 66-67 0.6189288173546464 68-69 0.6208178487513338 70-71 0.6172697549975558 72-73 0.6087444741725058 74-75 0.6071511172552998 76-77 0.5895913384460927 78-79 0.5664137445267369 80-81 0.5615515419752632 82-83 0.5755139479507787 84-85 0.5852876321336395 86-87 0.5759410333100298 88-89 0.589542059366179 90-91 0.602863837302818 92-93 0.6110113118485305 94-95 0.6101899938499709 96-97 0.6072332490551557 98-99 0.6114876762876952 100-101 0.5888521522473888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3043888.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.935191782966044 #Duplication Level Percentage of deduplicated Percentage of total 1 73.01789093188195 35.7314449633934 2 13.997760801168258 13.699662186745062 3 5.19398793698105 7.6250638744373935 4 2.5028504750907934 4.899098720106226 5 1.4122088661740988 3.4553355851917273 6 0.8265676029442854 2.426894650299908 7 0.5725780130123486 1.9613450414228233 8 0.3942765810976 1.5435200089234575 9 0.318057074165478 1.4007765529995049 >10 1.5811777973322978 13.865885132878548 >50 0.11325781429556937 3.848936873615801 >100 0.06386854220601194 5.5575489331366175 >500 0.003566230164286549 1.2108319049878054 >1k 0.0016821840396613384 1.6565344765277938 >5k 1.3457472317290707E-4 0.4428708324414881 >10k+ 1.3457472317290707E-4 0.674250262892517 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10437 0.3428838380387189 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10040 0.3298413082215903 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8160 0.2680781947298981 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5290 0.1737908884952403 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4931 0.16199676203592248 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4225 0.13880274175659552 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3874 0.12727143705681682 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.2852719942389474E-5 0.0 4 0.0 0.0 0.0 6.570543988477895E-5 0.0 5 0.0 0.0 0.0 6.570543988477895E-5 0.0 6 0.0 0.0 0.0 6.570543988477895E-5 0.0 7 0.0 0.0 0.0 6.570543988477895E-5 0.0 8 0.0 0.0 0.0 6.570543988477895E-5 0.0 9 0.0 0.0 0.0 6.570543988477895E-5 0.0 10-11 3.2852719942389474E-5 0.0 0.0 6.570543988477895E-5 0.0 12-13 9.855815982716841E-5 0.0 0.0 6.570543988477895E-5 0.0 14-15 1.314108797695579E-4 0.0 0.0 8.213179985597368E-5 0.0 16-17 2.46395399567921E-4 1.6426359971194737E-5 0.0 1.1498451979836315E-4 0.0 18-19 3.285271994238947E-4 3.2852719942389474E-5 0.0 1.314108797695579E-4 0.0 20-21 3.285271994238947E-4 3.2852719942389474E-5 0.0 2.1354267962553158E-4 0.0 22-23 3.285271994238947E-4 3.2852719942389474E-5 0.0 3.778062793374789E-4 0.0 24-25 4.270853592510631E-4 3.2852719942389474E-5 0.0 6.734807588189842E-4 0.0 26-27 4.270853592510631E-4 3.2852719942389474E-5 0.0 0.001314108797695579 0.0 28-29 4.270853592510631E-4 3.2852719942389474E-5 0.0 0.003613799193662842 0.0 30-31 4.270853592510631E-4 3.2852719942389474E-5 0.0 0.011728421019433041 0.0 32-33 4.270853592510631E-4 3.2852719942389474E-5 0.0 0.02761271111157835 0.0 34-35 4.763644391646473E-4 3.2852719942389474E-5 0.0 0.05161162302949386 3.2852719942389474E-5 36-37 5.420698790494263E-4 3.2852719942389474E-5 0.0 0.09213545307843127 3.2852719942389474E-5 38-39 5.58496239020621E-4 3.2852719942389474E-5 0.0 0.152255930573004 3.2852719942389474E-5 40-41 5.58496239020621E-4 3.2852719942389474E-5 0.0 0.21899623113596822 3.2852719942389474E-5 42-43 5.58496239020621E-4 3.2852719942389474E-5 0.0 0.2876748421755334 3.2852719942389474E-5 44-45 5.58496239020621E-4 3.2852719942389474E-5 0.0 0.3716299679883097 3.2852719942389474E-5 46-47 5.749225989918157E-4 3.2852719942389474E-5 0.0 0.4777442534022277 3.2852719942389474E-5 48-49 7.227598387325683E-4 3.2852719942389474E-5 0.0 0.5932051376397556 3.2852719942389474E-5 50-51 7.227598387325683E-4 3.2852719942389474E-5 0.0 0.7189489232192512 3.2852719942389474E-5 52-53 7.227598387325683E-4 3.2852719942389474E-5 0.0 0.8419823594034997 3.2852719942389474E-5 54-55 7.227598387325683E-4 3.2852719942389474E-5 0.0 0.9838404041147375 3.2852719942389474E-5 56-57 7.227598387325683E-4 3.2852719942389474E-5 0.0 1.1535411289771504 3.2852719942389474E-5 58-59 7.227598387325683E-4 3.2852719942389474E-5 0.0 1.3408509117286838 3.2852719942389474E-5 60-61 7.227598387325683E-4 3.2852719942389474E-5 0.0 1.5499749005219638 3.2852719942389474E-5 62-63 7.227598387325683E-4 3.2852719942389474E-5 0.0 1.7629426575485039 3.2852719942389474E-5 64-65 7.227598387325683E-4 3.2852719942389474E-5 0.0 1.9824645322035503 3.2852719942389474E-5 66-67 7.227598387325683E-4 3.2852719942389474E-5 0.0 2.2373523598765788 3.2852719942389474E-5 68-69 7.227598387325683E-4 3.2852719942389474E-5 0.0 2.5330596920780266 3.2852719942389474E-5 70-71 7.227598387325683E-4 3.2852719942389474E-5 1.6426359971194737E-5 2.8636073337783783 3.2852719942389474E-5 72-73 7.227598387325683E-4 3.2852719942389474E-5 3.2852719942389474E-5 3.198951472590319 3.2852719942389474E-5 74-75 7.227598387325683E-4 3.2852719942389474E-5 3.2852719942389474E-5 3.5470753194598483 3.2852719942389474E-5 76-77 7.227598387325683E-4 3.2852719942389474E-5 3.2852719942389474E-5 3.9225490556814178 3.2852719942389474E-5 78-79 7.227598387325683E-4 3.2852719942389474E-5 3.2852719942389474E-5 4.362463402069984 3.2852719942389474E-5 80-81 7.227598387325683E-4 3.2852719942389474E-5 3.2852719942389474E-5 4.843115121187113 3.2852719942389474E-5 82-83 7.227598387325683E-4 3.2852719942389474E-5 3.2852719942389474E-5 5.328793306455428 3.2852719942389474E-5 84-85 7.227598387325683E-4 3.2852719942389474E-5 3.2852719942389474E-5 5.82404805958695 3.2852719942389474E-5 86-87 7.227598387325683E-4 3.2852719942389474E-5 3.2852719942389474E-5 6.345338593272814 3.2852719942389474E-5 88-89 7.227598387325683E-4 3.2852719942389474E-5 3.2852719942389474E-5 6.909354089243757 3.2852719942389474E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13925 0.0 24.552061 1 TACATGG 14045 0.0 23.588984 2 GAGTACT 9610 0.0 22.900484 12-13 TTTGCGT 545 0.0 22.579636 3 ACATGGG 14335 0.0 22.15465 3 GTACTTT 10445 0.0 20.93417 14-15 CATGGGG 6240 0.0 20.631533 4 GTATCAA 27180 0.0 20.045574 1 AGTACTT 10150 0.0 19.653805 12-13 CAACGCA 30200 0.0 17.850765 5 TCAACGC 30385 0.0 17.789104 4 ATCAACG 30340 0.0 17.783997 3 TATCAAC 30570 0.0 17.727316 2 AACGCAG 30685 0.0 17.596865 6 CATGGGA 8405 0.0 17.56969 4 ACTTTTT 12600 0.0 17.543161 16-17 ACTATCG 135 0.00960226 17.528908 8 AGAGTAC 20115 0.0 17.434793 10-11 GGTATCA 21580 0.0 17.358955 1 CTTTGCG 750 0.0 17.03866 2 >>END_MODULE