FastQCFastQC Report
Fri 27 May 2016
SRR939004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043888
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT106710.350571374505238No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102170.33565623965139324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85820.28194204254558647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52600.1728053068969686No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49800.16360654531309957No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39460.12963683289266884No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35250.11580583779692288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG140000.024.4676651
TACATGG142800.023.7584532
ACATGGG143500.022.5513633
GAGTACT98300.020.13002412-13
CATGGGG63950.019.3660164
CATGGGA85500.019.0356774
AGTACTT105000.018.86813212-13
GTACTTT106200.018.49854514-15
TTTGCGT5950.018.3421923
AGTACAT106650.018.1971242
CTTTGCG6250.017.4617652
GTATCAA275200.017.1906591
AGAGTAC203450.016.89827710-11
GGTATCA224650.016.774971
CGCGGAA5000.016.72178882-83
GTGTAGC14000.015.9941631
TACTTTT123650.015.84957714-15
ACTTTTT126950.015.7546516-17
ATGGGGA33700.015.629085
GAGTACA144900.015.48617651
Produced by FastQC (version 0.11.5)