##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939004_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3043888 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19173241591018 33.0 31.0 34.0 30.0 34.0 2 32.41568447984945 34.0 31.0 34.0 31.0 34.0 3 32.512577006775544 34.0 31.0 34.0 30.0 34.0 4 36.075515919113975 37.0 35.0 37.0 35.0 37.0 5 35.99054038781979 37.0 35.0 37.0 35.0 37.0 6 36.03704965491503 37.0 35.0 37.0 35.0 37.0 7 35.978252484979734 37.0 35.0 37.0 35.0 37.0 8 35.946745412446184 37.0 35.0 37.0 35.0 37.0 9 37.62795214541402 39.0 37.0 39.0 35.0 39.0 10-11 37.554403939961 39.0 37.0 39.0 35.0 39.0 12-13 37.48103593167686 39.0 37.0 39.0 34.0 39.0 14-15 38.74984854896107 40.0 38.0 41.0 34.0 41.0 16-17 38.74531388802742 40.0 38.0 41.0 34.5 41.0 18-19 38.84029471518006 40.0 38.0 41.0 35.0 41.0 20-21 38.840163140036694 40.0 38.0 41.0 34.5 41.0 22-23 38.77947595312311 40.0 38.0 41.0 34.0 41.0 24-25 38.713758686259155 40.0 38.0 41.0 34.0 41.0 26-27 38.56203579106722 40.0 38.0 41.0 34.0 41.0 28-29 38.38758866949112 40.0 38.0 41.0 34.0 41.0 30-31 38.22716144615045 40.0 38.0 41.0 34.0 41.0 32-33 38.08082409733866 40.0 37.5 41.0 33.0 41.0 34-35 38.05463325194619 40.0 38.0 41.0 33.0 41.0 36-37 37.950280036584786 40.0 37.0 41.0 33.0 41.0 38-39 37.82853294864989 40.0 37.0 41.0 33.0 41.0 40-41 37.67499181967274 40.0 37.0 41.0 33.0 41.0 42-43 37.50409361316842 40.0 37.0 41.0 32.5 41.0 44-45 37.255112047486634 40.0 37.0 41.0 31.5 41.0 46-47 36.944349135053585 40.0 36.0 41.0 31.0 41.0 48-49 36.656149634940576 40.0 36.0 41.0 30.5 41.0 50-51 36.38654871664134 39.0 35.5 41.0 30.0 41.0 52-53 36.157029595044236 39.0 35.0 41.0 29.5 41.0 54-55 35.90146056622319 39.0 35.0 40.0 29.0 41.0 56-57 35.62373254206462 38.5 35.0 40.0 28.0 41.0 58-59 35.31767019680093 38.0 34.5 40.0 28.0 41.0 60-61 34.90275151385333 38.0 34.0 40.0 27.0 41.0 62-63 34.595601743559556 37.0 34.0 40.0 26.0 41.0 64-65 34.34296826952897 37.0 34.0 39.5 26.0 41.0 66-67 33.89385516155654 36.0 33.0 39.0 26.0 41.0 68-69 33.483265810042944 36.0 33.0 39.0 25.5 40.0 70-71 33.021443627360796 35.0 32.5 38.5 25.0 40.0 72-73 32.50943694380345 35.0 32.0 37.5 23.5 39.5 74-75 32.04743308558002 35.0 31.5 37.0 22.5 39.0 76-77 30.068015971678328 33.0 29.5 35.0 20.0 37.0 78-79 31.05461928954022 34.0 31.0 36.0 20.0 38.0 80-81 31.057737012662752 34.0 31.0 35.5 20.0 37.0 82-83 30.750212064307227 34.0 31.0 35.0 19.5 37.0 84-85 30.363494156158175 34.0 30.5 35.0 18.0 36.0 86-87 29.803657854691103 34.0 30.0 35.0 10.5 36.0 88-89 29.252779175843525 34.0 29.0 35.0 6.0 35.5 90-91 28.807769865382696 34.0 29.0 35.0 2.0 35.0 92-93 28.460331654778365 34.0 29.0 35.0 2.0 35.0 94-95 28.08273152625852 33.0 28.5 35.0 2.0 35.0 96-97 27.47848918882692 33.0 27.0 35.0 2.0 35.0 98-99 26.610945113617845 33.0 26.0 35.0 2.0 35.0 100-101 22.33117594996925 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11078491052448669 1101 2 0.13582801956769686 1101 3 0.15105129623987068 1101 4 0.09536048283780474 1101 5 0.0897814605973295 1101 6 0.16251640564602354 1101 7 0.13577274214748059 1101 8 0.20273085252323853 1101 9 0.19129598113920565 1101 10-11 0.239939232592576 1101 12-13 0.24053174633687036 1101 14-15 0.24923335539962466 1101 16-17 0.24626679938072726 1101 18-19 0.25215741253720125 1101 20-21 0.22063841255418026 1101 22-23 0.2835641590546629 1101 24-25 0.26228212825459707 1101 26-27 0.29389025393585655 1101 28-29 0.3561929933156307 1101 30-31 0.2073961792704111 1101 32-33 0.2238352470235938 1101 34-35 0.30821192323209345 1101 36-37 0.3633551289824126 1101 38-39 0.31984898854553734 1101 40-41 0.41464195058110676 1101 42-43 0.24987589362589802 1101 44-45 0.4798838684193356 1101 46-47 0.40409855301107456 1101 48-49 0.27950694829200984 1101 50-51 0.3524722943807532 1101 52-53 0.33729200915857405 1101 54-55 0.2317829637145863 1101 56-57 0.11198764753015666 1101 58-59 0.2974854725283791 1101 60-61 0.4567940070238308 1101 62-63 0.34021417919700525 1101 64-65 0.3421912172639452 1101 66-67 0.38101236433151087 1101 68-69 0.46291834545380794 1101 70-71 0.34205924877377214 1101 72-73 0.2896679592888489 1101 74-75 0.3209015673289102 1101 76-77 0.11457041139418322 1101 78-79 0.35107866473725124 1101 80-81 0.41874652118063693 1101 82-83 0.4082610245625631 1101 84-85 0.20454913767784788 1101 86-87 0.41486496354918145 1101 88-89 0.42775476845386606 1101 90-91 0.38194290012632237 1101 92-93 0.5063018801225674 1101 94-95 0.4318247583120147 1101 96-97 0.3895445111070508 1101 98-99 0.21835511455967804 1101 100-101 0.7761024732007336 1102 1 0.1263650742844291 1102 2 0.09031103699509657 1102 3 0.09219800405680445 1102 4 0.052909712726098235 1102 5 0.09129483281636652 1102 6 0.03749982584135836 1102 7 0.07174849195904187 1102 8 0.14496907762683975 1102 9 0.21012093398785225 1102 10-11 0.22593876027033133 1102 12-13 0.20916372643463177 1102 14-15 0.20042855712327423 1102 16-17 0.19278827548478006 1102 18-19 0.11157134474925812 1102 20-21 0.22696106040559982 1102 22-23 0.2377301382660164 1102 24-25 0.25099395022146354 1102 26-27 0.1956009662689624 1102 28-29 0.24250858868428793 1102 30-31 0.19281727395807735 1102 32-33 0.2415385963058938 1102 34-35 0.30458215916675613 1102 36-37 0.2760356870990748 1102 38-39 0.28615882546495897 1102 40-41 0.25552111696470803 1102 42-43 0.3419903767727277 1102 44-45 0.30799189433882646 1102 46-47 0.35439148985253865 1102 48-49 0.2651554693734326 1102 50-51 0.23608530071181377 1102 52-53 0.27105107952488083 1102 54-55 0.1796317947170465 1102 56-57 0.22233829826726748 1102 58-59 0.06580688457776773 1102 60-61 0.0026827007293235283 1102 62-63 0.01671000108623133 1102 64-65 0.06790625166906494 1102 66-67 0.09910068859043264 1102 68-69 0.04483972644639067 1102 70-71 0.1907514661325891 1102 72-73 0.2703629199987603 1102 74-75 0.24361153235765443 1102 76-77 -0.012976974496321958 1102 78-79 -0.07819205031332643 1102 80-81 0.24662582667284028 1102 82-83 0.2739846642906869 1102 84-85 0.18004434813622439 1102 86-87 0.24623913011070897 1102 88-89 0.356836231099976 1102 90-91 0.3599990029502287 1102 92-93 0.33545774969692843 1102 94-95 0.28821679871174055 1102 96-97 0.17510374387456906 1102 98-99 0.44200745951019726 1102 100-101 1.0995726829312176 1103 1 0.047465549414624775 1103 2 0.054140243863862736 1103 3 0.04518231651358917 1103 4 0.09251996640375637 1103 5 0.031692318242114936 1103 6 0.01812542451175858 1103 7 0.09440376565052588 1103 8 0.08677926660052293 1103 9 0.06267832705184162 1103 10-11 0.08412127624016819 1103 12-13 0.10345486896735423 1103 14-15 0.11096388739903063 1103 16-17 0.04676217821312889 1103 18-19 0.10342847582556658 1103 20-21 0.0972606652038479 1103 22-23 0.07554961416377637 1103 24-25 0.04139968223280732 1103 26-27 -0.0422740154933976 1103 28-29 0.19187867641527134 1103 30-31 0.13400357701375043 1103 32-33 0.05460880012342528 1103 34-35 0.09871027304576074 1103 36-37 0.03373799804609945 1103 38-39 0.05403012798143436 1103 40-41 0.08476033772580394 1103 42-43 0.1080322059922807 1103 44-45 0.09126369545655422 1103 46-47 0.1461372920232904 1103 48-49 0.09011851832643458 1103 50-51 -0.0013932105891854007 1103 52-53 0.11380930866577899 1103 54-55 0.04987462494522532 1103 56-57 0.08180506135555987 1103 58-59 0.08141989134631444 1103 60-61 0.093356129596188 1103 62-63 0.05113156654191897 1103 64-65 0.09822101749774248 1103 66-67 0.20544707055959321 1103 68-69 0.17212418924477646 1103 70-71 0.19796310523663152 1103 72-73 0.1765484935487862 1103 74-75 0.15119395063715757 1103 76-77 -0.06083793706316598 1103 78-79 0.08351112335342137 1103 80-81 0.18711719630383783 1103 82-83 0.24414724889748385 1103 84-85 0.21815784652813974 1103 86-87 0.10036496031985465 1103 88-89 0.25997215386118455 1103 90-91 0.3733457845616108 1103 92-93 0.3789924265893596 1103 94-95 0.2988345948434592 1103 96-97 0.04322529854625756 1103 98-99 0.04518049190658857 1103 100-101 0.4816068618567506 1104 1 -0.005287033586817813 1104 2 0.04971142198186129 1104 3 0.02088826209286765 1104 4 0.0018190209865736051 1104 5 0.021661319730938544 1104 6 -0.011670553911486081 1104 7 -0.0254200690373807 1104 8 0.06261763542544685 1104 9 0.06531556965571639 1104 10-11 -0.024856570921961918 1104 12-13 0.03827707018584903 1104 14-15 0.044339532993525665 1104 16-17 0.05425755836260748 1104 18-19 0.010716991413353583 1104 20-21 0.09101520088704262 1104 22-23 -0.01550296360058212 1104 24-25 -0.005700847210789561 1104 26-27 0.010772217925641314 1104 28-29 -0.12383567342649826 1104 30-31 -0.14748110952221793 1104 32-33 -0.07586600329913296 1104 34-35 -0.059503334876687575 1104 36-37 -0.15153745976174804 1104 38-39 -0.029769720891472673 1104 40-41 -2.2882651143874E-4 1104 42-43 -0.057392982355466415 1104 44-45 -0.03449624189801881 1104 46-47 -0.01880546173182296 1104 48-49 0.03335184010494885 1104 50-51 -0.09765094463043056 1104 52-53 -0.010141592485631179 1104 54-55 -0.037275332534697725 1104 56-57 -0.13176271016821062 1104 58-59 -0.0951423164060543 1104 60-61 0.10337116749018094 1104 62-63 0.11250575074240743 1104 64-65 0.04191046378901575 1104 66-67 0.0810091211996351 1104 68-69 0.10537214990186072 1104 70-71 -0.09677687386493972 1104 72-73 -0.163015382586849 1104 74-75 -0.01027612195871086 1104 76-77 -0.17688558948082544 1104 78-79 -0.007280348689313598 1104 80-81 -0.01698617861854501 1104 82-83 -0.004698848357541863 1104 84-85 -0.04172526688950029 1104 86-87 -0.24744793635759166 1104 88-89 -0.11749556953134643 1104 90-91 -0.08070048127743235 1104 92-93 -0.1413614751580745 1104 94-95 -0.1388004157678573 1104 96-97 -0.02499037926873271 1104 98-99 0.018839613025232183 1104 100-101 0.4088429069155701 1105 1 -0.03980113352185555 1105 2 0.03622499018221248 1105 3 0.042564818689982076 1105 4 0.019979410243145423 1105 5 0.10693002147822739 1105 6 0.036087302145737965 1105 7 0.019365428377227545 1105 8 0.04229528083539691 1105 9 -0.03508748714927634 1105 10-11 0.037272885434312286 1105 12-13 0.01784485404525782 1105 14-15 0.1269477681388409 1105 16-17 8.953785256693436E-5 1105 18-19 0.0765959663034721 1105 20-21 0.035543665309475614 1105 22-23 -0.04399189268836068 1105 24-25 0.10684884451936938 1105 26-27 0.0778926044279089 1105 28-29 0.10777459087002939 1105 30-31 -0.005447996605347782 1105 32-33 -0.008605629989823171 1105 34-35 0.03781313835474265 1105 36-37 0.07075286307340178 1105 38-39 0.04402394787941688 1105 40-41 0.08171958183498162 1105 42-43 0.024028592635978896 1105 44-45 0.0465598850411979 1105 46-47 0.16496936201749435 1105 48-49 0.18947598198342774 1105 50-51 0.1391803732993253 1105 52-53 0.13143856568152756 1105 54-55 0.021984334504715264 1105 56-57 0.08609142955607751 1105 58-59 0.015103450696543064 1105 60-61 7.841718097267858E-5 1105 62-63 -0.06723408180420876 1105 64-65 -0.12941575520198256 1105 66-67 0.01766090694594169 1105 68-69 -0.19546481818712635 1105 70-71 -0.02270107967591173 1105 72-73 0.06760373686146437 1105 74-75 0.17105138333293013 1105 76-77 -0.08832002508773229 1105 78-79 0.09158793388655084 1105 80-81 0.015365119170336783 1105 82-83 0.14667416998037197 1105 84-85 -0.08713646110009421 1105 86-87 -0.05391157314072359 1105 88-89 0.05886999519647418 1105 90-91 0.2779151747227431 1105 92-93 0.14797141060001806 1105 94-95 0.15446291938016543 1105 96-97 0.051877380657838756 1105 98-99 0.2993219494636179 1105 100-101 1.0446099664777435 1106 1 -0.007059794434560729 1106 2 -0.09240443248508257 1106 3 -0.07386163847343141 1106 4 -0.04254663934790415 1106 5 -0.013764303860412497 1106 6 -0.046859667341664135 1106 7 -0.02301472045122921 1106 8 0.013579064619179348 1106 9 -0.08505659648379549 1106 10-11 0.06640462720678642 1106 12-13 0.051968262253879516 1106 14-15 0.017399036961400327 1106 16-17 -0.009182445235943248 1106 18-19 -0.1485047741503962 1106 20-21 -0.10418807000823449 1106 22-23 -0.05890683962095977 1106 24-25 0.07759642167687275 1106 26-27 0.014928783390907086 1106 28-29 0.01451140701063025 1106 30-31 -0.02314932767028921 1106 32-33 -0.06587847986119044 1106 34-35 -0.10386378677919339 1106 36-37 -0.11806961472681365 1106 38-39 -0.043142133247762615 1106 40-41 -0.003084112596404509 1106 42-43 0.0324983981631064 1106 44-45 0.05218331169201207 1106 46-47 0.10894447197904356 1106 48-49 -0.11118534336443275 1106 50-51 -0.06315561801439884 1106 52-53 -0.0013579270753041328 1106 54-55 -0.07109356299360314 1106 56-57 0.029652397125488505 1106 58-59 0.026803174985829514 1106 60-61 0.03446053471304111 1106 62-63 0.06551492231762523 1106 64-65 0.13605464739098494 1106 66-67 0.1694835714733145 1106 68-69 0.11367403535422937 1106 70-71 0.02071966963020344 1106 72-73 0.15070930916867553 1106 74-75 0.19868060544143162 1106 76-77 0.006729630504402451 1106 78-79 0.05574830715644197 1106 80-81 0.1551404241580272 1106 82-83 0.26278964574272834 1106 84-85 0.24611422817669393 1106 86-87 0.2554962481913563 1106 88-89 0.3215896877651936 1106 90-91 0.4115659442230495 1106 92-93 0.32627048756848964 1106 94-95 0.39781687338534866 1106 96-97 0.3567147666620585 1106 98-99 0.2619070716892189 1106 100-101 0.9461934012431144 1107 1 -0.036349030883151556 1107 2 -0.029228645348553073 1107 3 -0.04991895610118746 1107 4 -0.06204325985491721 1107 5 -0.06305507846874292 1107 6 0.014741177977427355 1107 7 -0.08671682572377648 1107 8 0.022169909670765264 1107 9 -0.02279902383361332 1107 10-11 0.028933718488765692 1107 12-13 0.019261580398811873 1107 14-15 0.03603178014669339 1107 16-17 0.01579498394183787 1107 18-19 -0.02819839056456175 1107 20-21 0.001224803274155306 1107 22-23 0.007848011155246581 1107 24-25 -0.06051616715741659 1107 26-27 -0.04625887655542016 1107 28-29 -0.05023519789185826 1107 30-31 0.018470432555126592 1107 32-33 0.015993471684552674 1107 34-35 -0.12989491110707974 1107 36-37 0.018426290201539075 1107 38-39 0.03002879419567961 1107 40-41 0.007362722807123134 1107 42-43 -0.0010961250965451086 1107 44-45 0.0688358414074699 1107 46-47 -0.16328656184263224 1107 48-49 -0.12082052085469996 1107 50-51 -0.04610561890608267 1107 52-53 -0.018685575162187718 1107 54-55 -0.17929847485036277 1107 56-57 -0.01398881708443156 1107 58-59 -0.030748966400032884 1107 60-61 -0.08302711542472707 1107 62-63 -0.17977568955786438 1107 64-65 -0.175960776561503 1107 66-67 -0.10331875493755405 1107 68-69 -0.043232134672607 1107 70-71 -0.16419254380395643 1107 72-73 0.033260335946025066 1107 74-75 -0.1757358412758272 1107 76-77 -0.4486802333350255 1107 78-79 -0.14884744663099525 1107 80-81 0.022867938046047698 1107 82-83 -0.03598227860656422 1107 84-85 -0.1646037357456578 1107 86-87 0.0060972450972016645 1107 88-89 0.03199288624576013 1107 90-91 0.19685199734975356 1107 92-93 0.28667067660609646 1107 94-95 0.2201493365809739 1107 96-97 0.25469955448518533 1107 98-99 0.3816686903682758 1107 100-101 0.768403146042349 1108 1 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-1.9325891655174736 2316 86-87 -1.9130622153855583 2316 88-89 -2.0098210389868605 2316 90-91 -2.301550427288152 2316 92-93 -2.658175080825419 2316 94-95 -2.70942099508407 2316 96-97 -2.908136563318422 2316 98-99 -3.2622549230677933 2316 100-101 -3.9096067419417153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 9.0 4 23.0 5 63.0 6 158.0 7 440.0 8 867.0 9 1693.0 10 2644.0 11 4168.0 12 6023.0 13 8387.0 14 10129.0 15 13568.0 16 17702.0 17 25343.0 18 24374.0 19 16474.0 20 15283.0 21 16277.0 22 17562.0 23 19308.0 24 22398.0 25 26898.0 26 32560.0 27 39671.0 28 48401.0 29 59259.0 30 72553.0 31 88677.0 32 110174.0 33 141489.0 34 191127.0 35 276076.0 36 440160.0 37 704866.0 38 548031.0 39 41046.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.96904380183283 19.468657839004017 14.56161880067824 23.000679558484915 2 15.679617646904223 21.0279418953654 39.232980976961045 24.059459480769334 3 19.688700766913893 24.7109946226668 30.000282533391502 25.600022077027802 4 13.240993098300594 16.78156357921185 36.62013845450293 33.35730486798463 5 13.755474511306131 37.611711897877676 33.866425045147984 14.766388545668207 6 31.9627069064302 35.81817070798925 17.20680918614614 15.012313199434407 7 28.46898440415679 33.11639587264709 20.523291264330357 17.891328458865765 8 26.209177210199584 34.779433408850785 19.619282969675623 19.392106411274 9 26.4780438702081 17.09363156594461 20.63679741173131 35.791527152115975 10-11 25.351737646063192 26.423179827904313 26.808180852909175 21.41690167312332 12-13 26.332621305383114 23.880149335323768 27.5161405413077 22.27108881798542 14-15 22.944517816193912 25.133054739705507 26.34237448502888 25.580052959071704 16-17 22.2771861679436 28.5568744602782 26.7783982165793 22.387541155198896 18-19 22.066235682784647 27.29195358042083 28.906648339229303 21.73516239756522 20-21 23.118229054419874 26.135899218368085 28.67183352344107 22.074038203770964 22-23 22.663514557697262 26.138231761484 28.7553615638946 22.442892116924146 24-25 22.760249391567626 26.3083924244256 28.807728799482767 22.123629384524005 26-27 22.544226331586444 26.316129239972035 29.017739811714492 22.12190461672703 28-29 22.078768995442672 26.825050724599592 28.987876689286857 22.10830359067088 30-31 22.31663911418554 26.379666400340618 28.81411865351156 22.48957583196228 32-33 22.198566438712593 26.560307080943847 28.82451653937333 22.416609940970233 34-35 22.875372291049263 26.40453784822796 28.544298671916206 22.17579118880657 36-37 22.815889244653224 26.173188620425623 28.7566061034967 22.254316031424445 38-39 22.393896162611284 26.551784639405323 28.892817808806097 22.161501389177296 40-41 22.533079726613316 26.502351239174583 28.72055037411043 22.244018660101673 42-43 22.298667240497096 26.642870225346165 28.262336875148353 22.796125659008386 44-45 22.564702001854215 26.51054017276645 28.184276767991356 22.740481057387978 46-47 22.734563813897303 26.887965469055352 27.80846870201948 22.569002015027866 48-49 22.822010529654673 26.816698887061722 27.78140527573447 22.579885307549134 50-51 22.393672399388805 27.01947618673722 27.931669903225202 22.655181510648774 52-53 22.814407100392657 27.25303953364907 27.339425760737583 22.59312760522069 54-55 22.37064898577083 26.812320295621916 27.63015590586776 23.186874812739497 56-57 22.616124509180363 27.17161406727186 27.40828506173683 22.803976361810946 58-59 22.987649498527293 26.767375700359196 27.445555177931357 22.799419623182153 60-61 22.74950103898877 26.81233316632855 27.95709345970941 22.481072334973266 62-63 22.495555026991795 26.82877950831305 27.888411137334884 22.78725432736027 64-65 22.329121550888235 27.018479530165678 27.708963046031855 22.94343587291424 66-67 22.380607656675835 27.03816116514647 27.325807643337424 23.25542353484028 68-69 22.14688060917798 26.983222998453105 27.69227812438862 23.177618267980293 70-71 22.527405738975943 26.829732237191383 27.471855074825356 23.17100694900732 72-73 22.511521448883794 26.56209755418071 27.629022487029747 23.297358509905752 74-75 22.12221671756648 27.31476979442082 27.246255446980967 23.316758041031733 76-77 22.323524680838048 26.77083857580958 27.357453164745166 23.54818357860721 78-79 22.31144118641821 26.799325007239926 27.501142043990175 23.388091762351692 80-81 22.326149976608864 27.021165036295685 27.364722355093225 23.28796263200223 82-83 22.30438504964703 26.797996509727035 27.490433287952776 23.40718515267316 84-85 22.166732153088418 26.742031901305175 27.360254385181058 23.730981560425352 86-87 22.0427311803511 26.620120145561792 27.58243289416172 23.75471577992539 88-89 22.0773390271714 26.62452826110522 27.261092185365648 24.037040526357735 90-91 22.060208287148484 26.522732011395878 27.44569279747095 23.971366903984684 92-93 22.162855532135218 26.57098421492512 27.348197436962202 23.91796281597746 94-95 21.988604705560782 26.389259394563798 27.37554732631424 24.24658857356118 96-97 21.628154901236087 26.27782021272227 27.68321629501869 24.410808591022956 98-99 21.712576152604825 26.345795246080012 27.203727601015544 24.737901000299615 100-101 21.736406512206116 25.726144623119673 27.896940225054568 24.640508639619643 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 630.0 1 458.5 2 357.5 3 443.0 4 404.5 5 423.5 6 714.0 7 1431.5 8 2304.0 9 2829.0 10 2811.0 11 2663.0 12 2839.0 13 3110.5 14 3407.0 15 3702.5 16 3955.5 17 4183.0 18 4491.0 19 4829.5 20 5236.0 21 6035.0 22 7140.5 23 8571.0 24 10262.0 25 12515.5 26 15931.5 27 19764.5 28 23021.0 29 26759.5 30 31292.5 31 36282.0 32 41193.5 33 46168.5 34 52535.5 35 58280.5 36 64430.5 37 71270.5 38 76490.0 39 79451.0 40 81920.5 41 86972.0 42 92310.5 43 98307.5 44 103989.0 45 107230.5 46 110204.5 47 120376.0 48 143765.0 49 144744.5 50 124997.0 51 116781.5 52 111618.5 53 108744.0 54 106048.5 55 98836.5 56 89173.5 57 79294.5 58 71798.5 59 64101.5 60 54988.5 61 45243.5 62 36302.0 63 28711.0 64 22270.5 65 17101.0 66 11855.0 67 9083.5 68 6674.0 69 3816.5 70 2448.5 71 1692.5 72 1261.0 73 948.5 74 728.5 75 572.5 76 354.5 77 154.5 78 67.5 79 36.0 80 21.0 81 12.0 82 5.5 83 3.0 84 3.5 85 5.0 86 5.0 87 3.0 88 1.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4109218210394075 2 0.0 3 0.0 4 0.0 5 2.9567447948150523E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.198761583869053E-4 16-17 0.0024475276357080154 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0021847058761689 36-37 0.006784086668103426 38-39 0.011826979179260209 40-41 0.0022175585961112894 42-43 5.092171591070369E-4 44-45 3.285271994238947E-4 46-47 3.121008394527E-4 48-49 2.792481195103105E-4 50-51 5.58496239020621E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.1498451979836316E-4 60-61 0.002069721356370537 62-63 0.0 64-65 2.1354267962553158E-4 66-67 0.0015605041972634997 68-69 0.0015933569172058891 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.1253988320201006 78-79 1.1498451979836316E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1475579916212423 88-89 0.44277253302355407 90-91 0.3395492869645664 92-93 0.0 94-95 0.0 96-97 4.2708535925106315E-4 98-99 0.0 100-101 1.314108797695579E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3043888.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.572840049523215 #Duplication Level Percentage of deduplicated Percentage of total 1 71.65095468663579 34.086394067029595 2 14.823842745917304 14.104245997416175 3 5.426861686472936 7.745136689443882 4 2.573019737643778 4.896234256927745 5 1.4241974212529835 3.38765580601058 6 0.8811633537789538 2.5151665972096557 7 0.6220140782173755 2.071368337611078 8 0.40814998132036473 1.5533483022055679 9 0.32225555897015923 1.379755094576137 >10 1.671068542836627 14.273444672126237 >50 0.12219967516294765 4.056108858533046 >100 0.06853421383329228 5.856744856847421 >500 0.0036642271296320195 1.2024302774251885 >1k 0.0017975453841781433 1.7296919578020524 >5k 1.382727218598572E-4 0.4552651850141854 >10k+ 1.382727218598572E-4 0.6870090438214351 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10671 0.350571374505238 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10217 0.33565623965139324 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8582 0.28194204254558647 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5260 0.1728053068969686 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4980 0.16360654531309957 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3946 0.12963683289266884 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3525 0.11580583779692288 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.2852719942389474E-5 0.0 8 0.0 0.0 0.0 3.2852719942389474E-5 0.0 9 0.0 0.0 0.0 6.570543988477895E-5 0.0 10-11 0.0 0.0 0.0 6.570543988477895E-5 0.0 12-13 3.2852719942389474E-5 0.0 0.0 1.6426359971194736E-4 0.0 14-15 3.2852719942389474E-5 0.0 0.0 1.8068995968314208E-4 0.0 16-17 3.2852719942389474E-5 0.0 0.0 2.1354267962553158E-4 0.0 18-19 3.2852719942389474E-5 0.0 0.0 2.628217595391158E-4 0.0 20-21 3.2852719942389474E-5 0.0 0.0 3.121008394527E-4 0.0 22-23 6.570543988477895E-5 0.0 0.0 5.092171591070368E-4 0.0 24-25 1.6426359971194736E-4 0.0 0.0 7.884652786173472E-4 0.0 26-27 1.9711631965433683E-4 0.0 0.0 0.0014126669575227472 0.0 28-29 1.9711631965433683E-4 0.0 0.0 0.0038109155133171785 0.0 30-31 2.1354267962553158E-4 0.0 0.0 0.011909110979116183 0.0 32-33 2.299690395967263E-4 0.0 0.0 0.027842680151175074 0.0 34-35 2.299690395967263E-4 0.0 0.0 0.05233438286822643 0.0 36-37 2.299690395967263E-4 0.0 0.0 0.09381094179549312 0.0 38-39 2.299690395967263E-4 0.0 0.0 0.15496627996825113 0.0 40-41 2.299690395967263E-4 0.0 0.0 0.2225278985297751 0.0 42-43 2.299690395967263E-4 0.0 0.0 0.2922906493274391 0.0 44-45 2.299690395967263E-4 0.0 0.0 0.37810195381696043 0.0 46-47 2.299690395967263E-4 0.0 0.0 0.48623668150733534 0.0 48-49 2.299690395967263E-4 0.0 0.0 0.6027981318629332 0.0 50-51 2.299690395967263E-4 0.0 0.0 0.7302174061594908 0.0 52-53 2.299690395967263E-4 0.0 0.0 0.8546142302213484 0.0 54-55 2.299690395967263E-4 0.0 0.0 0.9976221201305698 0.0 56-57 2.299690395967263E-4 0.0 0.0 1.1686205274307069 0.0 58-59 2.299690395967263E-4 0.0 0.0 1.356800907260714 0.0 60-61 2.299690395967263E-4 0.0 0.0 1.5672061521317473 0.0 62-63 2.299690395967263E-4 0.0 0.0 1.7826542895139375 0.0 64-65 2.299690395967263E-4 0.0 0.0 2.0037366683662476 0.0 66-67 2.299690395967263E-4 0.0 0.0 2.259182992278297 0.0 68-69 2.299690395967263E-4 0.0 0.0 2.5565822395567777 0.0 70-71 2.299690395967263E-4 0.0 0.0 2.8900373469720306 0.0 72-73 2.299690395967263E-4 0.0 0.0 3.228601052338325 0.0 74-75 2.299690395967263E-4 0.0 0.0 3.5801908611617774 0.0 76-77 2.299690395967263E-4 0.0 0.0 3.9584406522184787 0.0 78-79 2.299690395967263E-4 0.0 0.0 4.399964781884222 0.0 80-81 2.299690395967263E-4 0.0 0.0 4.883064028637059 0.0 82-83 2.299690395967263E-4 0.0 0.0 5.371879648659872 0.0 84-85 2.299690395967263E-4 0.0 0.0 5.87012399930615 0.0 86-87 2.299690395967263E-4 0.0 0.0 6.3946012468264275 0.0 88-89 2.299690395967263E-4 0.0 0.0 6.9602922315144315 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14000 0.0 24.467665 1 TACATGG 14280 0.0 23.758453 2 ACATGGG 14350 0.0 22.551363 3 GAGTACT 9830 0.0 20.130024 12-13 CATGGGG 6395 0.0 19.366016 4 CATGGGA 8550 0.0 19.035677 4 AGTACTT 10500 0.0 18.868132 12-13 GTACTTT 10620 0.0 18.498545 14-15 TTTGCGT 595 0.0 18.342192 3 AGTACAT 10665 0.0 18.197124 2 CTTTGCG 625 0.0 17.461765 2 GTATCAA 27520 0.0 17.190659 1 AGAGTAC 20345 0.0 16.898277 10-11 GGTATCA 22465 0.0 16.77497 1 CGCGGAA 500 0.0 16.721788 82-83 GTGTAGC 1400 0.0 15.994163 1 TACTTTT 12365 0.0 15.849577 14-15 ACTTTTT 12695 0.0 15.75465 16-17 ATGGGGA 3370 0.0 15.62908 5 GAGTACA 14490 0.0 15.4861765 1 >>END_MODULE