FastQCFastQC Report
Fri 27 May 2016
SRR939003_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939003_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335618
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112070.4798301777088548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86510.3703944737538416No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59670.25547842155694983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56700.24276230102696586No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA33240.14231779340628475No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31560.1351248363388191No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28310.12120988963092423No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25750.11024919314716705No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG96100.023.9537371
TACATGG95250.023.2231772
GAGTACT85600.022.51028612-13
ACATGGG94700.021.9075533
AGTACTT89150.020.28627812-13
GTACTTT95750.020.12515614-15
CATGGGG35800.020.1108444
GTATCAA211250.019.6367531
TTTGCGT4001.8189894E-1218.9465333
TGTACGC1507.8720896E-418.9465332
GGTATCA167600.018.1242851
ATCAACG234950.017.438553
CAACGCA235800.017.3957775
TCAACGC235850.017.3720054
CATGGGA64800.017.2507024
TATCAAC237850.017.2458442
AACGCAG238800.017.227516
ACTTTTT117300.016.81325316-17
AGTACAT72600.016.2454782
AGAGTAC175450.016.14908210-11
Produced by FastQC (version 0.11.5)