##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939003_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2335618 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86208789279754 33.0 31.0 34.0 30.0 34.0 2 31.937488065257245 33.0 31.0 34.0 30.0 34.0 3 31.98212164831749 34.0 31.0 34.0 30.0 34.0 4 35.5180521814783 37.0 35.0 37.0 33.0 37.0 5 35.56605660685951 37.0 35.0 37.0 33.0 37.0 6 35.65391215515551 37.0 35.0 37.0 33.0 37.0 7 35.58267961627287 37.0 35.0 37.0 33.0 37.0 8 35.57096537190585 37.0 35.0 37.0 33.0 37.0 9 37.190155667579205 39.0 37.0 39.0 34.0 39.0 10-11 37.082070355683165 39.0 37.0 39.0 33.0 39.0 12-13 36.97983488738312 39.0 37.0 39.0 33.0 39.0 14-15 38.19635552560393 40.0 38.0 41.0 33.0 41.0 16-17 38.119739614954156 40.0 38.0 41.0 33.0 41.0 18-19 38.056207607579665 40.0 38.0 41.0 33.0 41.0 20-21 38.170168024051875 40.0 38.0 41.0 33.0 41.0 22-23 38.104875240728575 40.0 38.0 41.0 33.0 41.0 24-25 38.026317659822794 40.0 38.0 41.0 33.0 41.0 26-27 37.91462859080552 40.0 38.0 41.0 33.0 41.0 28-29 37.786678943217595 40.0 38.0 41.0 33.0 41.0 30-31 37.587761140734486 40.0 37.0 41.0 32.0 41.0 32-33 37.3779817590034 40.0 37.0 41.0 31.5 41.0 34-35 37.05726385907285 40.0 36.0 41.0 31.0 41.0 36-37 36.79418787661338 40.0 36.0 41.0 30.0 41.0 38-39 36.557720055248765 39.5 35.5 41.0 30.0 41.0 40-41 36.4133085975532 39.0 35.0 41.0 30.0 41.0 42-43 36.201727979489796 39.0 35.0 41.0 30.0 41.0 44-45 36.10855649339918 39.0 35.0 41.0 29.5 41.0 46-47 35.871813798318044 39.0 35.0 41.0 27.5 41.0 48-49 35.62305265672725 39.0 35.0 41.0 27.0 41.0 50-51 34.50701655835843 38.0 34.0 40.0 25.0 40.5 52-53 34.60357430024944 38.5 34.5 39.5 25.0 40.5 54-55 35.107129890247464 39.0 35.0 40.5 26.0 41.0 56-57 35.02984028210093 39.0 35.0 41.0 25.5 41.0 58-59 34.842438061361065 39.0 35.0 40.5 25.0 41.0 60-61 34.54771841970734 38.0 34.0 40.0 24.0 41.0 62-63 34.18260413303888 38.0 34.0 40.0 23.0 41.0 64-65 33.807231961733464 37.0 34.0 40.0 22.0 41.0 66-67 33.405567605661545 37.0 33.0 39.5 21.5 41.0 68-69 32.974282823646675 36.0 33.0 39.0 20.0 41.0 70-71 32.54006798200733 36.0 32.0 39.0 18.5 40.5 72-73 32.10248593734078 35.0 32.0 38.5 17.0 40.0 74-75 31.63364043263924 35.0 31.0 37.0 12.5 39.5 76-77 31.201583264044032 35.0 31.0 37.0 8.0 39.0 78-79 30.803694568204218 35.0 31.0 36.5 7.0 39.0 80-81 30.347262480422742 35.0 30.5 36.0 2.0 37.5 82-83 29.879231963446077 34.0 30.0 35.5 2.0 37.0 84-85 29.45794967327705 34.0 29.5 35.0 2.0 37.0 86-87 29.010698025105132 34.0 29.0 35.0 2.0 36.0 88-89 28.53701654123234 34.0 29.0 35.0 2.0 36.0 90-91 28.078561220199536 34.0 28.5 35.0 2.0 35.5 92-93 27.58980728012886 34.0 27.0 35.0 2.0 35.0 94-95 27.06893721490415 33.5 26.0 35.0 2.0 35.0 96-97 26.441082831182154 33.0 25.0 35.0 2.0 35.0 98-99 25.620745558563087 33.0 22.0 35.0 2.0 35.0 100-101 22.486640794856008 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13351314350879662 1101 2 0.17806648477957765 1101 3 0.3190721662648386 1101 4 0.21096370402042908 1101 5 0.059092571772822566 1101 6 0.1376008386789067 1101 7 0.21746853354703433 1101 8 0.1748409152010737 1101 9 0.22999697369842664 1101 10-11 0.18361242837728042 1101 12-13 0.17006294701861435 1101 14-15 0.3062005504815559 1101 16-17 0.30651171810362854 1101 18-19 0.30365307820085263 1101 20-21 0.28767061337691757 1101 22-23 0.34470594184298164 1101 24-25 0.3673699507336252 1101 26-27 0.1332981217882434 1101 28-29 0.1746690431486897 1101 30-31 0.14958630198970013 1101 32-33 0.11249339439600448 1101 34-35 0.06479901911485086 1101 36-37 0.04393686980623812 1101 38-39 0.2093894368638587 1101 40-41 0.13254498852121088 1101 42-43 0.07200202929612232 1101 44-45 0.15365963046736653 1101 46-47 0.07972534925988128 1101 48-49 0.014391815800344432 1101 50-51 0.05558969431915983 1101 52-53 0.016952568866742013 1101 54-55 0.11044145150750495 1101 56-57 0.05162606680350734 1101 58-59 0.11966908180452407 1101 60-61 0.4101729471152211 1101 62-63 0.19226874541980976 1101 64-65 -0.020026772045007135 1101 66-67 0.07291986964191466 1101 68-69 0.030340252566965376 1101 70-71 0.03523058661002665 1101 72-73 0.12392240407243804 1101 74-75 -0.19655105088773084 1101 76-77 -0.17457697067333555 1101 78-79 -0.1140537722471997 1101 80-81 -0.04710420942835114 1101 82-83 -0.18673076051628712 1101 84-85 -0.28180481667202173 1101 86-87 -0.1795842334657145 1101 88-89 -0.23965769875110254 1101 90-91 -0.23709516987800328 1101 92-93 -0.22307639431972248 1101 94-95 -0.47385183325108926 1101 96-97 -0.5769076678107403 1101 98-99 -0.5154833711879938 1101 100-101 -0.12539529657296455 1102 1 0.3226321373653285 1102 2 0.3214069687117487 1102 3 0.33410059349080967 1102 4 0.2633439241143165 1102 5 0.2491979721160078 1102 6 0.27449184385986314 1102 7 0.315338510657007 1102 8 0.3563897149957356 1102 9 0.43239771626912216 1102 10-11 0.40370963079306676 1102 12-13 0.3916744980200093 1102 14-15 0.5055885270759788 1102 16-17 0.48870794395929096 1102 18-19 0.4042818072344545 1102 20-21 0.4233377930133102 1102 22-23 0.5372687290055396 1102 24-25 0.4839161561774361 1102 26-27 0.43746700573058916 1102 28-29 0.5211412238526876 1102 30-31 0.5504162729927131 1102 32-33 0.5320115421290623 1102 34-35 0.43182631345585065 1102 36-37 0.2892176405220468 1102 38-39 0.5323782617944133 1102 40-41 0.5611469044686643 1102 42-43 0.3952696501381112 1102 44-45 0.5788838011878212 1102 46-47 0.5283812100035377 1102 48-49 0.3859439014039694 1102 50-51 0.35999500161974396 1102 52-53 0.24119608603206188 1102 54-55 0.39891169170345364 1102 56-57 0.3777580943688932 1102 58-59 0.3134834634694883 1102 60-61 0.4391195964198289 1102 62-63 0.4375344700070656 1102 64-65 0.23777149256107322 1102 66-67 0.27233273872843 1102 68-69 0.33479645118471524 1102 70-71 0.22488117346435388 1102 72-73 0.2404118546342886 1102 74-75 0.29954270861982124 1102 76-77 0.22736287357710694 1102 78-79 0.13206924976771717 1102 80-81 0.14033470716188745 1102 82-83 0.15062634274994124 1102 84-85 0.042628657866277564 1102 86-87 -0.13193579953536627 1102 88-89 -0.1632943922758443 1102 90-91 -0.06629594386855686 1102 92-93 -0.16170456354884166 1102 94-95 -0.18744766989881256 1102 96-97 -0.2952064153311724 1102 98-99 -0.500949032271734 1102 100-101 -0.33887839788983243 1103 1 0.3204534929417484 1103 2 0.34020164362750904 1103 3 0.42513080947711046 1103 4 0.3894600374656889 1103 5 0.24237589842656604 1103 6 0.3215823813398373 1103 7 0.23351450358635617 1103 8 0.2812143297004823 1103 9 0.39820117425951196 1103 10-11 0.3639013117299257 1103 12-13 0.4249303119735046 1103 14-15 0.4842832185635686 1103 16-17 0.4460956697505978 1103 18-19 0.5998383448249172 1103 20-21 0.6109404003159682 1103 22-23 0.5784486439367598 1103 24-25 0.6116447094559021 1103 26-27 0.6687540852201792 1103 28-29 0.5314190783519379 1103 30-31 0.5747774286022818 1103 32-33 0.5408294176193138 1103 34-35 0.48458000069000917 1103 36-37 0.5455044438143162 1103 38-39 0.7405890555854953 1103 40-41 0.5707939242996289 1103 42-43 0.4615542438518574 1103 44-45 0.6732245181960437 1103 46-47 0.6045950972045517 1103 48-49 0.5903846018403627 1103 50-51 0.5551985590411661 1103 52-53 0.44392822987562397 1103 54-55 0.3719454430153917 1103 56-57 0.3475062024172857 1103 58-59 0.31639734514655515 1103 60-61 0.49391272463062563 1103 62-63 0.40729252115880854 1103 64-65 0.4688381665930095 1103 66-67 0.5591901656742309 1103 68-69 0.4686830998064906 1103 70-71 0.4122832615160519 1103 72-73 0.24961309199826331 1103 74-75 0.272559335698201 1103 76-77 0.3111677677935383 1103 78-79 0.33404958451886557 1103 80-81 0.12410645770047424 1103 82-83 0.11577300352439224 1103 84-85 0.1321176928328569 1103 86-87 0.19970812688566753 1103 88-89 0.3655002826399141 1103 90-91 0.32494694405057345 1103 92-93 0.29754859891042074 1103 94-95 0.15086973605600562 1103 96-97 0.2083587396155231 1103 98-99 0.19037726723663795 1103 100-101 0.1661456312766063 1104 1 0.2001424142495658 1104 2 0.3105084315791089 1104 3 0.2162966036931948 1104 4 0.34213059440381954 1104 5 0.3152798716523435 1104 6 0.23027713805484495 1104 7 0.3256289805450763 1104 8 0.27812499648156574 1104 9 0.2924846205823499 1104 10-11 0.3837266785882534 1104 12-13 0.41464497772480513 1104 14-15 0.43611021590331944 1104 16-17 0.4620645189739818 1104 18-19 0.41923807021751713 1104 20-21 0.4690406749233631 1104 22-23 0.47516150757702036 1104 24-25 0.40972932286017993 1104 26-27 0.44146797863818676 1104 28-29 0.5469421196288735 1104 30-31 0.5924490582255544 1104 32-33 0.6207762124361054 1104 34-35 0.6061752980383304 1104 36-37 0.5465162004435129 1104 38-39 0.6884273804803698 1104 40-41 0.6291531451011494 1104 42-43 0.6814396810783236 1104 44-45 0.7126778778459268 1104 46-47 0.7941117452780091 1104 48-49 0.667616645091023 1104 50-51 0.7378375790360181 1104 52-53 0.8186055661838623 1104 54-55 0.8750332744751006 1104 56-57 0.8239950893991832 1104 58-59 0.6188666663514013 1104 60-61 0.7147318270076894 1104 62-63 0.8696708786633138 1104 64-65 0.8270291903637101 1104 66-67 0.799565605475145 1104 68-69 0.8497866782841328 1104 70-71 0.7419385005103081 1104 72-73 0.8633803622291083 1104 74-75 0.8496257563624106 1104 76-77 0.818715609551024 1104 78-79 0.7108795853770147 1104 80-81 0.5763831246531907 1104 82-83 0.7773751660576487 1104 84-85 0.6257292091805802 1104 86-87 0.6054019396377477 1104 88-89 0.5973476609974213 1104 90-91 0.6589997630671363 1104 92-93 0.6355057773194162 1104 94-95 0.8252992820956493 1104 96-97 0.6469204833724937 1104 98-99 0.8981248429682047 1104 100-101 1.2328569931576645 1105 1 0.18318102152941051 1105 2 0.11645131787933138 1105 3 0.14727939234223442 1105 4 0.18473298837935204 1105 5 0.13289636583611042 1105 6 0.20917809918458374 1105 7 0.08791559601544918 1105 8 0.19262646108384018 1105 9 0.2380170874489238 1105 10-11 0.2649171248495037 1105 12-13 0.2104104908483393 1105 14-15 0.2412416166122071 1105 16-17 0.3773113098327414 1105 18-19 0.3528924555063284 1105 20-21 0.329284765654279 1105 22-23 0.25609575181413646 1105 24-25 0.29177177730534254 1105 26-27 0.14448099879528087 1105 28-29 0.231303789556641 1105 30-31 0.339862331607506 1105 32-33 0.37216555846762134 1105 34-35 0.4880688939801203 1105 36-37 0.37041286948640817 1105 38-39 0.4499618470128439 1105 40-41 0.45553285667952537 1105 42-43 0.4599640715440998 1105 44-45 0.4126494293319425 1105 46-47 0.4939605918727068 1105 48-49 0.43786367577737195 1105 50-51 0.39868859896486697 1105 52-53 0.4429654746193421 1105 54-55 0.4766630063147801 1105 56-57 0.5305521824241666 1105 58-59 0.4321036193170684 1105 60-61 0.5041373605744539 1105 62-63 0.5084638594886854 1105 64-65 0.47646327995337145 1105 66-67 0.37972032734957395 1105 68-69 0.3946340238889263 1105 70-71 0.26475592472892373 1105 72-73 0.17460793214711146 1105 74-75 0.22677717523204421 1105 76-77 0.2751465863439826 1105 78-79 0.13911614138667971 1105 80-81 0.18390527562548442 1105 82-83 0.18809066272404706 1105 84-85 0.35675864016068104 1105 86-87 0.33314552590373125 1105 88-89 0.3457347170889591 1105 90-91 0.26399638480609866 1105 92-93 0.175903700908691 1105 94-95 0.2201143130003267 1105 96-97 0.16448082482082071 1105 98-99 0.1534141784142271 1105 100-101 0.4314240026399503 1106 1 0.14114807208786928 1106 2 0.17469370941236662 1106 3 0.19097262041786678 1106 4 0.20624343029704306 1106 5 0.1765621512896942 1106 6 0.17131185452446118 1106 7 0.18543295347389233 1106 8 0.13139282374724104 1106 9 0.28477016788325216 1106 10-11 0.1983207417870858 1106 12-13 0.22814299846366737 1106 14-15 0.22263323197642393 1106 16-17 0.2059184138128387 1106 18-19 0.281532490632884 1106 20-21 0.2721743822808449 1106 22-23 0.24628958820122193 1106 24-25 0.35552556210029707 1106 26-27 0.20600507054859207 1106 28-29 0.12784053065379197 1106 30-31 0.1285449942736392 1106 32-33 0.1749308600162962 1106 34-35 0.19599020683515533 1106 36-37 0.37233385302997846 1106 38-39 0.3594149155553694 1106 40-41 0.33177120499957624 1106 42-43 0.317637486046614 1106 44-45 0.38841302029052116 1106 46-47 0.2076883061767134 1106 48-49 0.2158322814177538 1106 50-51 -0.04341661604808422 1106 52-53 0.11126650188816711 1106 54-55 0.3241346260698137 1106 56-57 0.1358060724158463 1106 58-59 0.14774316962397194 1106 60-61 0.3977263066863017 1106 62-63 0.236962565111682 1106 64-65 0.06979563754903495 1106 66-67 0.24498162684920288 1106 68-69 0.04930978930939034 1106 70-71 0.13859761738984844 1106 72-73 0.14775303958498398 1106 74-75 0.16130822655889432 1106 76-77 0.14804608231449023 1106 78-79 0.22773522864507 1106 80-81 0.28830158367806646 1106 82-83 0.2591418980994149 1106 84-85 0.22897207057991054 1106 86-87 0.19733198937302987 1106 88-89 0.29119021887730057 1106 90-91 0.30413350644094805 1106 92-93 0.25396881310327757 1106 94-95 0.21517464103113682 1106 96-97 0.21835069190705525 1106 98-99 0.290748493583596 1106 100-101 0.4858129926599837 1107 1 0.1153458044101221 1107 2 0.1423005984453667 1107 3 0.06523348686149433 1107 4 0.1416913274781706 1107 5 0.13949602278483297 1107 6 0.04117167180229586 1107 7 0.03634160877954429 1107 8 0.11316704083341023 1107 9 0.05822285514147296 1107 10-11 0.09820926206843694 1107 12-13 0.19286183311236016 1107 14-15 0.2830361587775201 1107 16-17 0.23129887688484274 1107 18-19 0.11835361348376239 1107 20-21 0.1877461518857828 1107 22-23 0.1793504459044044 1107 24-25 0.18930235605753154 1107 26-27 0.2041352392158089 1107 28-29 0.14851793090149812 1107 30-31 0.18497726088224908 1107 32-33 0.2759573552765531 1107 34-35 0.20972675571649546 1107 36-37 0.22695154041696952 1107 38-39 0.3890370316956151 1107 40-41 0.0979322524172872 1107 42-43 0.2680196464821947 1107 44-45 0.05950726962269215 1107 46-47 0.2997025811995613 1107 48-49 0.42729568977065213 1107 50-51 0.357070273671674 1107 52-53 0.4473548319087115 1107 54-55 0.5735263065690148 1107 56-57 0.4864796331100578 1107 58-59 0.44708806532000267 1107 60-61 0.3588164901498416 1107 62-63 0.287144436644283 1107 64-65 0.3305407294368834 1107 66-67 0.3652248141780561 1107 68-69 0.4836551805575482 1107 70-71 0.24619993329613976 1107 72-73 0.48061286700797723 1107 74-75 0.42666663561025686 1107 76-77 0.5491258786932924 1107 78-79 0.6062806699947174 1107 80-81 0.5465739875116853 1107 82-83 0.5084690007426964 1107 84-85 0.47083974801683937 1107 86-87 0.5269943349812287 1107 88-89 0.5142834942739576 1107 90-91 0.652761061168242 1107 92-93 0.5869726237820743 1107 94-95 0.6535630742982157 1107 96-97 0.6566737669211626 1107 98-99 0.59185279442978 1107 100-101 0.8852687827768797 1108 1 0.212453068178025 1108 2 0.10872028013594814 1108 3 0.20356555514982944 1108 4 0.17717162289629584 1108 5 0.1655679978212774 1108 6 0.16090275937955312 1108 7 0.24493427633776577 1108 8 0.20820806443553863 1108 9 0.23844116576423602 1108 10-11 0.3310564566805496 1108 12-13 0.3266060048693262 1108 14-15 0.42412180834800495 1108 16-17 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-1.9709302659937258 2316 76-77 -1.9243925604273997 2316 78-79 -1.7916674765758032 2316 80-81 -1.7023122125292964 2316 82-83 -1.575658741440396 2316 84-85 -1.5464572840096977 2316 86-87 -1.5434995687640125 2316 88-89 -1.8407779673949243 2316 90-91 -1.9334908915274625 2316 92-93 -1.9689138450403618 2316 94-95 -2.153331557482044 2316 96-97 -2.224691075534775 2316 98-99 -2.3719917599866847 2316 100-101 -2.206647033756063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6259.0 3 1643.0 4 1604.0 5 1788.0 6 2107.0 7 2629.0 8 3327.0 9 4098.0 10 5415.0 11 7220.0 12 9944.0 13 14979.0 14 13334.0 15 13590.0 16 17749.0 17 23899.0 18 20611.0 19 14320.0 20 14001.0 21 14848.0 22 16185.0 23 17725.0 24 20575.0 25 23342.0 26 27129.0 27 31508.0 28 37222.0 29 44547.0 30 53457.0 31 65341.0 32 81190.0 33 103252.0 34 137998.0 35 196747.0 36 297987.0 37 465723.0 38 448602.0 39 73699.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.479428163956115 19.482332018866465 14.153801214807944 22.884438602369478 2 15.04448784024745 21.26948579401678 40.42421897196514 23.26180739377063 3 19.067278326780443 25.398594793174713 30.82240685169042 24.71172002835442 4 12.352679983507532 16.989001305511007 38.41532565341983 32.242993057561634 5 12.995741813471371 37.91462023097254 34.871607146072 14.21803080948409 6 30.476030131981645 36.746957499237446 18.121943362019035 14.655069006761876 7 26.948023572837425 33.85802146505973 21.814085438683374 17.379869523419465 8 25.044747933540854 34.94168845156299 20.906517304105158 19.107046310791 9 26.05245862869981 17.08075811467003 21.634792935710717 35.231990320919444 10-11 24.271770623544196 26.73969417215144 28.162715187159222 20.82582001714514 12-13 25.653188028829277 24.707370692064966 28.31282937866516 21.326611900440597 14-15 22.501328570684993 25.77756995693096 27.487757978867535 24.23334349351651 16-17 21.240135128101155 28.97675656999451 28.12062824156751 21.662480060336826 18-19 20.90427286710111 27.80139929649576 30.513061100328613 20.781266736074517 20-21 22.073444184206654 26.888167040242266 30.140877596668176 20.897511178882908 22-23 21.558931075990348 26.9847133119272 29.99853445257733 21.457821159505126 24-25 21.537004764087357 26.92539691621073 30.28147178650169 21.256126533200224 26-27 21.503666795371263 26.87362679981093 30.46834188340392 21.15436452141389 28-29 21.02621162869577 27.358351294061634 30.43401218779776 21.181424889444834 30-31 21.30917590799239 27.22734518279828 30.187297533551583 21.27618137565775 32-33 21.066363884678623 27.311670853127527 30.111047436439886 21.510917825753964 34-35 21.666153699772828 27.09835497638384 30.024349639953087 21.211141683890254 36-37 21.65898111810154 26.931057283462152 30.100999103382552 21.308962495053756 38-39 21.37393890191756 27.235463854366852 30.217143550420957 21.17345369329463 40-41 21.5965329100195 27.14463392293162 30.079276713172547 21.179556453876337 42-43 21.18770579180565 27.3531825726275 29.70871551491382 21.750396120653026 44-45 21.44215565342427 27.38016013432814 29.480715610633425 21.696968601614167 46-47 21.588570604871112 27.56262619634403 29.057049084394095 21.791754114390766 48-49 21.693234751782665 27.569028281405437 28.977858745540512 21.759878221271382 50-51 21.482992986492423 27.832655307857134 29.115696408121845 21.568655297528597 52-53 21.647078771676632 28.032705999966 28.66562678932887 21.654588439028494 54-55 21.299151279341988 27.625021542362127 28.920919682973885 22.154907495322 56-57 21.57951434240392 28.030326496406012 28.544773872027452 21.845385289162618 58-59 21.79551424686654 27.56850294667087 28.708652299719507 21.927330506743083 60-61 21.67696956211888 27.461231123450936 29.15092747557026 21.710871838859923 62-63 21.596987721635326 27.535231023954342 29.037605288612134 21.830175965798198 64-65 21.410721521436134 27.753861938768903 28.886421869374836 21.948994670420134 66-67 21.34446232848484 27.725815063527083 28.651330294983364 22.278392313004712 68-69 21.29800286224771 27.7088307595417 28.830376416353083 22.162789961857506 70-71 21.359949446402812 27.682506707692678 28.63172109084538 22.32582275505913 72-73 21.4147382955385 27.269653740032467 28.869064592138393 22.446543372290638 74-75 21.332571771925846 27.85067944391096 28.48617718698901 22.33057159717419 76-77 21.47527517095045 27.46468357976332 28.596715289990744 22.46332595929548 78-79 21.38630652901506 27.467931639985967 28.75189217897921 22.393869652019763 80-81 21.364584664406156 27.598888761249313 28.67271764304488 22.363808931299648 82-83 21.40522352014785 27.525599637694803 28.6278981457676 22.441278696389748 84-85 21.377801042479526 27.31169845159927 28.586344495011463 22.72415601090974 86-87 21.35474890383432 27.293578771175685 28.74619730551934 22.605475019470656 88-89 21.487967832746808 27.079498109605854 28.618708404228492 22.813825653418846 90-91 21.390240039526 27.062225041682197 28.79757731111316 22.74995760767865 92-93 21.502964437510087 27.24011405821273 28.660203367945336 22.596718136331845 94-95 21.466728621695037 26.956377447307915 28.646962011940047 22.929931919057005 96-97 21.334003903337205 26.77334669308489 28.78256035937043 23.110089044207477 98-99 21.364271911771638 26.750797183961243 28.507991423533717 23.3769394807334 100-101 21.672710107000416 26.13070570442962 29.168957206934383 23.02762698163559 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3189.0 1 1966.0 2 863.0 3 958.0 4 830.0 5 873.5 6 1421.5 7 2570.0 8 3882.5 9 4351.0 10 3876.0 11 3499.5 12 3702.5 13 3876.0 14 4088.0 15 4495.0 16 4742.0 17 4919.5 18 5063.5 19 5285.0 20 5729.5 21 6473.0 22 7408.0 23 8837.0 24 10931.0 25 13187.0 26 16217.5 27 20003.0 28 23413.5 29 26975.5 30 30852.0 31 35092.0 32 39789.5 33 44576.5 34 49632.5 35 54171.5 36 59506.5 37 64425.0 38 66811.0 39 68176.0 40 70439.0 41 73147.5 42 75411.0 43 78552.0 44 82251.0 45 84422.0 46 85810.5 47 91024.5 48 107881.0 49 108631.5 50 90981.0 51 82447.0 52 76407.0 53 71577.5 54 67110.0 55 61207.5 56 54425.0 57 47461.5 58 41661.5 59 36302.5 60 30986.0 61 25380.0 62 19961.5 63 15214.0 64 11229.5 65 8470.0 66 6240.0 67 4661.0 68 3396.5 69 2282.0 70 1604.0 71 1096.5 72 789.5 73 622.5 74 438.5 75 297.5 76 216.5 77 137.5 78 87.0 79 64.0 80 48.5 81 31.5 82 28.5 83 25.0 84 18.0 85 17.5 86 12.5 87 9.5 88 7.0 89 4.0 90 5.5 91 8.0 92 4.5 93 1.5 94 1.0 95 1.5 96 1.5 97 0.5 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32873526407143633 2 0.08815653929709397 3 0.09830374658869728 4 0.1041266165956933 5 0.1156010957271266 6 0.05797180874612201 7 0.06019820021938519 8 0.061311395956016784 9 0.0647366136071909 10-11 0.06118295029409775 12-13 0.059663009961389235 14-15 0.05747943370876572 16-17 0.059448933858190854 18-19 0.06799057037580632 20-21 0.070281184680029 22-23 0.08644393047150689 24-25 0.09515682787168106 26-27 0.09029730032907779 28-29 0.08796387080421542 30-31 0.08126328877410605 32-33 0.07302135880096831 34-35 0.32785755204832295 36-37 0.5088374896922356 38-39 0.4819709387408386 40-41 0.46182637742987076 42-43 0.493038673276195 44-45 0.503357141450357 46-47 0.49710611923696424 48-49 0.516330153304179 50-51 0.5120272236298916 52-53 0.5114920333718955 54-55 0.5013020108596525 56-57 0.4787597971928629 58-59 0.46201904592274934 60-61 0.4202527981887449 62-63 0.49365949397547026 64-65 0.5170580120550535 66-67 0.5273764802292156 68-69 0.5402638616417582 70-71 0.5368600516009039 72-73 0.5250216430940333 74-75 0.5275263335014544 76-77 0.5008952662635757 78-79 0.4815641941447617 80-81 0.4865949825699237 82-83 0.49710611923696424 84-85 0.49834776063551484 86-87 0.49699908118536507 88-89 0.5036782556051546 90-91 0.517379126209851 92-93 0.5241867462915596 94-95 0.53208615449958 96-97 0.5232662190478067 98-99 0.5259207627274666 100-101 0.5030146196852396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2335618.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.19666473803637 #Duplication Level Percentage of deduplicated Percentage of total 1 71.65665718153541 37.4023851115301 2 15.080667119586533 15.74321051333974 3 5.586772795090666 8.748327196587896 4 2.695783896596559 5.628437130273915 5 1.4746594452748147 3.84861523338941 6 0.8890626006931777 2.7843661499705097 7 0.5924348477841268 2.1646186190242376 8 0.3877248367946343 1.6190354653423518 9 0.27817456637683735 1.3067806121838383 >10 1.2483233147539614 11.210581115688402 >50 0.07022112679673938 2.501576462094466 >100 0.03539770747517052 3.319469476128862 >500 0.0020602806206303876 0.7577682833937063 >1k 0.001730635721329261 1.6100425226577963 >5k 2.4723367447560874E-4 0.8727067968602252 >10k+ 8.241122482520291E-5 0.48207931153453 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11207 0.4798301777088548 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8651 0.3703944737538416 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5967 0.25547842155694983 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5670 0.24276230102696586 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3324 0.14231779340628475 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3156 0.1351248363388191 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2831 0.12120988963092423 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2575 0.11024919314716705 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.563044127935304E-5 2 0.0 0.0 0.0 0.0 8.563044127935304E-5 3 0.0 0.0 0.0 0.0 8.563044127935304E-5 4 0.0 0.0 0.0 0.0 8.563044127935304E-5 5 0.0 0.0 0.0 0.0 8.563044127935304E-5 6 0.0 0.0 0.0 0.0 8.563044127935304E-5 7 0.0 0.0 0.0 0.0 8.563044127935304E-5 8 0.0 0.0 0.0 0.0 8.563044127935304E-5 9 0.0 0.0 0.0 0.0 8.563044127935304E-5 10-11 0.0 0.0 0.0 4.281522063967652E-5 8.563044127935304E-5 12-13 0.0 0.0 0.0 8.563044127935304E-5 1.7126088255870609E-4 14-15 0.0 0.0 0.0 1.0703805159919131E-4 1.7126088255870609E-4 16-17 0.0 0.0 0.0 1.9266849287854435E-4 1.7126088255870609E-4 18-19 0.0 0.0 0.0 2.5689132383805916E-4 1.7126088255870609E-4 20-21 0.0 0.0 0.0 3.6392937543725044E-4 1.7126088255870609E-4 22-23 0.0 0.0 0.0 5.351902579959565E-4 1.7126088255870609E-4 24-25 0.0 0.0 0.0 9.205272437530453E-4 1.7126088255870609E-4 26-27 0.0 0.0 0.0 0.0020979458113441493 1.7126088255870609E-4 28-29 0.0 0.0 0.0 0.006679174419789538 1.7126088255870609E-4 30-31 0.0 0.0 0.0 0.01982344715617023 1.7126088255870609E-4 32-33 0.0 0.0 0.0 0.045512579539976145 1.7126088255870609E-4 34-35 0.0 0.0 0.0 0.08575888694127207 1.7126088255870609E-4 36-37 0.0 0.0 0.0 0.1507952070929407 1.7126088255870609E-4 38-39 0.0 0.0 0.0 0.23978664319250836 1.9266849287854435E-4 40-41 2.140761031983826E-5 0.0 0.0 0.3405308573576672 2.1407610319838262E-4 42-43 4.281522063967652E-5 0.0 0.0 0.4482325448767735 2.1407610319838262E-4 44-45 4.281522063967652E-5 0.0 0.0 0.5795040113580217 2.354837135182209E-4 46-47 4.281522063967652E-5 0.0 0.0 0.7355868981999625 2.5689132383805916E-4 48-49 4.281522063967652E-5 0.0 0.0 0.90665511226579 2.5689132383805916E-4 50-51 4.281522063967652E-5 0.0 0.0 1.0842526474791683 2.5689132383805916E-4 52-53 4.281522063967652E-5 0.0 0.0 1.2595595683883238 2.5689132383805916E-4 54-55 4.281522063967652E-5 0.0 0.0 1.4569591431475524 2.5689132383805916E-4 56-57 4.281522063967652E-5 0.0 0.0 1.6970240852742187 2.5689132383805916E-4 58-59 4.281522063967652E-5 0.0 0.0 1.9629922358878893 2.5689132383805916E-4 60-61 4.281522063967652E-5 0.0 0.0 2.2562336820490336 2.5689132383805916E-4 62-63 4.281522063967652E-5 0.0 0.0 2.5444871550056556 2.5689132383805916E-4 64-65 4.281522063967652E-5 0.0 0.0 2.8487107052608778 2.5689132383805916E-4 66-67 4.281522063967652E-5 0.0 0.0 3.202107536420767 2.7829893415789737E-4 68-69 4.281522063967652E-5 0.0 0.0 3.5970351316011437 2.9970654447773564E-4 70-71 4.281522063967652E-5 0.0 0.0 4.03700433889446 2.9970654447773564E-4 72-73 4.281522063967652E-5 0.0 0.0 4.488940400356565 3.4252176511741217E-4 74-75 4.281522063967652E-5 0.0 0.0 4.960335979599403 4.06744596076927E-4 76-77 4.281522063967652E-5 0.0 0.0 5.459561452258032 4.709674270364417E-4 78-79 4.281522063967652E-5 0.0 0.0 6.034933794824324 4.709674270364417E-4 80-81 4.281522063967652E-5 2.140761031983826E-5 0.0 6.652586167772299 4.709674270364417E-4 82-83 4.281522063967652E-5 4.281522063967652E-5 0.0 7.281391905696908 4.709674270364417E-4 84-85 4.281522063967652E-5 4.281522063967652E-5 0.0 7.93363897692174 4.709674270364417E-4 86-87 4.281522063967652E-5 4.281522063967652E-5 0.0 8.624227078229403 4.9237503735628E-4 88-89 4.281522063967652E-5 4.281522063967652E-5 0.0 9.356731280543308 5.137826476761183E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9610 0.0 23.953737 1 TACATGG 9525 0.0 23.223177 2 GAGTACT 8560 0.0 22.510286 12-13 ACATGGG 9470 0.0 21.907553 3 AGTACTT 8915 0.0 20.286278 12-13 GTACTTT 9575 0.0 20.125156 14-15 CATGGGG 3580 0.0 20.110844 4 GTATCAA 21125 0.0 19.636753 1 TTTGCGT 400 1.8189894E-12 18.946533 3 TGTACGC 150 7.8720896E-4 18.946533 2 GGTATCA 16760 0.0 18.124285 1 ATCAACG 23495 0.0 17.43855 3 CAACGCA 23580 0.0 17.395777 5 TCAACGC 23585 0.0 17.372005 4 CATGGGA 6480 0.0 17.250702 4 TATCAAC 23785 0.0 17.245844 2 AACGCAG 23880 0.0 17.22751 6 ACTTTTT 11730 0.0 16.813253 16-17 AGTACAT 7260 0.0 16.245478 2 AGAGTAC 17545 0.0 16.149082 10-11 >>END_MODULE