FastQCFastQC Report
Fri 27 May 2016
SRR939003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939003_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335618
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109140.46728531806142953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91410.3913739318672831No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63120.2702496726776382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58890.25213883434705503No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32560.13940635840278676No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30130.12900225978734536No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28720.12296531367715097No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24680.10566796453872165No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCG3400.025.1216562
TTTGCGT3800.023.7260073
GTACATG93200.023.6199421
TACATGG92250.022.8901332
ACATGGG93050.021.4184273
GAGTACT93150.020.91372912-13
AGTACTT97350.020.84017412-13
CATGGGG34750.019.9366744
GTACTTT102850.018.84902614-15
GGTATCA169550.018.7729031
GTATCAA210200.018.7240641
GTATAGG5750.018.2308791
TACTTTT118050.017.06525814-15
TTGCGTT5050.016.913594
AGAGTAC179550.016.87627610-11
CATGGGA63450.016.4530244
ATCAACG238750.016.4169063
CAACGCA239150.016.409295
TCAACGC241200.016.250154
TATCAAC243500.016.1551232
Produced by FastQC (version 0.11.5)