##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939003_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2335618 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17094148101273 33.0 31.0 34.0 30.0 34.0 2 32.399365820951886 34.0 31.0 34.0 31.0 34.0 3 32.48905857036553 34.0 31.0 34.0 30.0 34.0 4 36.04680303029006 37.0 35.0 37.0 35.0 37.0 5 35.97098626573352 37.0 35.0 37.0 35.0 37.0 6 36.01288010282504 37.0 35.0 37.0 35.0 37.0 7 35.950401135802174 37.0 35.0 37.0 35.0 37.0 8 35.92404151706315 37.0 35.0 37.0 35.0 37.0 9 37.59716999954616 39.0 37.0 39.0 35.0 39.0 10-11 37.51285441369265 39.0 37.0 39.0 35.0 39.0 12-13 37.42547368619355 39.0 37.0 39.0 34.0 39.0 14-15 38.72763525542276 40.0 38.0 41.0 34.0 41.0 16-17 38.816186122901954 40.0 38.0 41.0 35.0 41.0 18-19 38.86777675116393 40.0 38.0 41.0 35.0 41.0 20-21 38.847079659430605 40.0 38.0 41.0 34.0 41.0 22-23 38.771186469705235 40.0 38.0 41.0 34.0 41.0 24-25 38.69432137447134 40.0 38.0 41.0 34.0 41.0 26-27 38.53010702092551 40.0 38.0 41.0 34.0 41.0 28-29 38.36700329420307 40.0 38.0 41.0 34.0 41.0 30-31 38.165374646025164 40.0 37.5 41.0 33.5 41.0 32-33 38.04350219085484 40.0 37.0 41.0 33.0 41.0 34-35 37.93574891099486 40.0 37.0 41.0 33.0 41.0 36-37 37.79846725791632 40.0 37.0 41.0 33.0 41.0 38-39 37.64581622508475 40.0 37.0 41.0 33.0 41.0 40-41 37.466427301039815 40.0 37.0 41.0 32.0 41.0 42-43 37.27016575484518 40.0 37.0 41.0 31.5 41.0 44-45 37.002076538201024 40.0 36.0 41.0 31.0 41.0 46-47 36.65770322886705 40.0 36.0 41.0 30.5 41.0 48-49 36.303688574073334 39.0 36.0 41.0 30.0 41.0 50-51 36.03426116770808 39.0 35.0 41.0 29.0 41.0 52-53 35.77572466901694 39.0 35.0 40.0 28.0 41.0 54-55 35.481352258802595 39.0 35.0 40.0 28.0 41.0 56-57 35.36448233401181 38.5 35.0 40.0 27.5 41.0 58-59 35.10936912628692 38.0 34.5 40.0 27.0 41.0 60-61 34.76026259431123 38.0 34.0 40.0 26.0 41.0 62-63 34.35510986813769 37.5 34.0 40.0 26.0 41.0 64-65 33.95634431657916 37.0 33.5 39.5 25.5 41.0 66-67 33.51334807318662 36.0 33.0 39.0 23.5 40.5 68-69 33.03921189167065 36.0 32.5 39.0 22.5 40.0 70-71 32.52276099944426 35.0 32.0 38.0 22.0 40.0 72-73 32.01387876784645 35.0 31.0 37.5 20.5 39.5 74-75 31.477299370016844 35.0 31.0 37.0 18.5 39.0 76-77 29.55440337418191 32.5 28.5 35.0 13.5 37.5 78-79 30.511264470474195 34.0 30.0 36.0 16.5 38.0 80-81 30.486690888664157 34.0 30.0 36.0 14.0 37.0 82-83 30.069571736474032 34.0 30.0 35.0 8.5 37.0 84-85 29.617543408211446 34.0 29.5 35.0 4.5 36.5 86-87 28.915093992253873 34.0 29.0 35.0 2.0 36.0 88-89 28.217242716916893 33.0 27.5 35.0 2.0 35.5 90-91 27.63457059330764 33.0 26.5 35.0 2.0 35.0 92-93 27.231058974541213 33.0 26.0 35.0 2.0 35.0 94-95 26.68877273595254 32.5 25.5 34.0 2.0 35.0 96-97 25.845291053588387 32.0 24.5 34.0 2.0 35.0 98-99 24.658167988087094 32.0 11.0 34.0 2.0 35.0 100-101 19.156221822232915 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13538652726536782 1101 2 0.1176554894986026 1101 3 0.16833494112690772 1101 4 0.10007178540663375 1101 5 0.038751138507578275 1101 6 0.17326149497069565 1101 7 0.2173274188653025 1101 8 0.14983207703488688 1101 9 0.2944315216948894 1101 10-11 0.19614558520004977 1101 12-13 0.2284032143737207 1101 14-15 0.24017046997021652 1101 16-17 0.24451728164655862 1101 18-19 0.25594002158593554 1101 20-21 0.25040408431959804 1101 22-23 0.2655522064965936 1101 24-25 0.23086867359071306 1101 26-27 0.2782999350194544 1101 28-29 0.31061675269368294 1101 30-31 0.22632763481257712 1101 32-33 0.23558605612473826 1101 34-35 0.3095624127925234 1101 36-37 0.4128817907815545 1101 38-39 0.23297691806015308 1101 40-41 0.3779629930821429 1101 42-43 0.37538405952914644 1101 44-45 0.3287334172092429 1101 46-47 0.38825150023973976 1101 48-49 0.41358965101990464 1101 50-51 0.29632996074627727 1101 52-53 0.352708708548235 1101 54-55 0.3682052905642337 1101 56-57 0.33897695626999536 1101 58-59 0.3872235868149332 1101 60-61 0.2882048970741451 1101 62-63 0.32257203985501803 1101 64-65 0.2968470141465147 1101 66-67 0.3650174480908248 1101 68-69 0.3253477922365846 1101 70-71 0.3133443477322331 1101 72-73 0.37637958067919897 1101 74-75 0.22885393566748036 1101 76-77 -0.042767409867874306 1101 78-79 0.44230384376712095 1101 80-81 0.4519618312587106 1101 82-83 0.4032070435381918 1101 84-85 0.2745735473904496 1101 86-87 0.5882284729569456 1101 88-89 0.4728201739950677 1101 90-91 0.40413273468644206 1101 92-93 0.3247803087423087 1101 94-95 0.311122220839696 1101 96-97 0.39137748601839917 1101 98-99 0.5553852142883855 1101 100-101 0.8142449740061615 1102 1 0.1594132031662241 1102 2 0.16351564679703046 1102 3 0.18046853747247127 1102 4 0.021092665229005547 1102 5 0.0881004487642798 1102 6 0.08355935299940853 1102 7 0.12596776882055138 1102 8 0.10844172624989312 1102 9 0.1276808105993652 1102 10-11 0.1247692959072495 1102 12-13 0.20498225578044327 1102 14-15 0.2576965545361034 1102 16-17 0.21800301603592231 1102 18-19 0.15595661658067428 1102 20-21 0.23107036229461642 1102 22-23 0.19161265431805674 1102 24-25 0.22319559056152372 1102 26-27 0.1802079168800148 1102 28-29 0.2877812655144254 1102 30-31 0.2910046353668463 1102 32-33 0.26788500499777257 1102 34-35 0.17153564617236583 1102 36-37 0.12661956995085433 1102 38-39 0.19029569795188905 1102 40-41 0.15288850921413655 1102 42-43 0.0916532391943079 1102 44-45 0.09308681536631269 1102 46-47 0.17555522293960024 1102 48-49 0.13418160554519432 1102 50-51 -0.057369450795206944 1102 52-53 -0.03672691780771942 1102 54-55 -0.04972582250631774 1102 56-57 -0.09651077615672676 1102 58-59 -0.15766764405795897 1102 60-61 -0.11433856245121632 1102 62-63 -0.024912435527411958 1102 64-65 0.019639575606085202 1102 66-67 -0.20777129651995097 1102 68-69 -0.35440719926124586 1102 70-71 -0.21978921874233492 1102 72-73 -0.004346328643542563 1102 74-75 -0.026275521886393705 1102 76-77 -0.13106262818608982 1102 78-79 -0.011453871522512316 1102 80-81 0.060237644496428544 1102 82-83 0.2152677325402479 1102 84-85 0.36103601449087463 1102 86-87 0.5675682576866699 1102 88-89 0.33869146100328607 1102 90-91 0.4070193787027847 1102 92-93 0.4420389926264683 1102 94-95 0.5974154189938901 1102 96-97 0.5383237304051463 1102 98-99 0.6815513169096192 1102 100-101 0.674008417887972 1103 1 0.1735401434523638 1103 2 0.172255634644344 1103 3 0.1659913789904479 1103 4 0.10728942819646647 1103 5 0.1470939857470981 1103 6 0.144152624832806 1103 7 0.15474642429202135 1103 8 0.2022615351213446 1103 9 0.22293684551070925 1103 10-11 0.19676841207584062 1103 12-13 0.1304896302487677 1103 14-15 0.26529032352465975 1103 16-17 0.2755575650057054 1103 18-19 0.23876278130475015 1103 20-21 0.24163988115888912 1103 22-23 0.18617156720542738 1103 24-25 0.22047504700521614 1103 26-27 0.2301598585471467 1103 28-29 0.06963896865755004 1103 30-31 0.23364839606949062 1103 32-33 0.24561963461716374 1103 34-35 0.13894933451131664 1103 36-37 0.14673833479645992 1103 38-39 0.07449997348633275 1103 40-41 0.24767335150546899 1103 42-43 0.14024297570207267 1103 44-45 0.0391498988331378 1103 46-47 0.09183042594462165 1103 48-49 0.044840612339655195 1103 50-51 0.05789266045707109 1103 52-53 0.1382821967036847 1103 54-55 0.2520672504650108 1103 56-57 0.3023391382221021 1103 58-59 0.21341946857531013 1103 60-61 0.14758542804759855 1103 62-63 0.040377847851033266 1103 64-65 0.2184922519580681 1103 66-67 0.21904470082855454 1103 68-69 0.1624369702134203 1103 70-71 0.20486415362688604 1103 72-73 0.19828748895574932 1103 74-75 0.21682893593827757 1103 76-77 0.04966984709424693 1103 78-79 0.4427244414642857 1103 80-81 0.34845948618012557 1103 82-83 0.3836094438588127 1103 84-85 0.5111348931297712 1103 86-87 0.5718161723969395 1103 88-89 0.36987469030229647 1103 90-91 0.5986152467909456 1103 92-93 0.6178424506168589 1103 94-95 0.9314495569820664 1103 96-97 0.7923302486617878 1103 98-99 1.0570769204007533 1103 100-101 1.0802648394755572 1104 1 -0.005659633429125677 1104 2 0.08155409855921647 1104 3 0.028911227956378127 1104 4 0.031633373961504674 1104 5 -0.0052710704616032444 1104 6 0.11187381250979911 1104 7 -0.01276666246675262 1104 8 0.09635575978452238 1104 9 0.1265372059329195 1104 10-11 0.1495005411413146 1104 12-13 0.17369867512991277 1104 14-15 0.17423587402668517 1104 16-17 0.21114839998381996 1104 18-19 0.1567622235084727 1104 20-21 0.21677804300562542 1104 22-23 0.19651225200762212 1104 24-25 0.12112509420335726 1104 26-27 0.1634437768514445 1104 28-29 0.22449329801479934 1104 30-31 0.08618808541987022 1104 32-33 0.09559775184020225 1104 34-35 0.2716349090414738 1104 36-37 0.23693206173373227 1104 38-39 0.29659406942729305 1104 40-41 0.29407964173116596 1104 42-43 0.2668170338845144 1104 44-45 0.3526116683345535 1104 46-47 0.1040553894733307 1104 48-49 0.1312220162699731 1104 50-51 0.011975085583650014 1104 52-53 0.009711013317435402 1104 54-55 0.20520828710794348 1104 56-57 0.025139704599858703 1104 58-59 0.13987488778066393 1104 60-61 0.12015922878561014 1104 62-63 0.10461881584210175 1104 64-65 0.02759462902834997 1104 66-67 0.08254980680984403 1104 68-69 -0.031113403081008073 1104 70-71 -0.06309795913377769 1104 72-73 -0.0010586021795759848 1104 74-75 0.21202909898641664 1104 76-77 0.024659120242791488 1104 78-79 0.22428262390657494 1104 80-81 0.352695090745474 1104 82-83 0.25171079115135697 1104 84-85 0.27053676389266457 1104 86-87 0.4023405008960417 1104 88-89 0.3287567378437046 1104 90-91 0.4230265571831211 1104 92-93 0.3453454541352663 1104 94-95 0.5490956566988814 1104 96-97 0.31012244809540945 1104 98-99 0.720034742420836 1104 100-101 1.1277131360515433 1105 1 -4.704760806362174E-5 1105 2 0.011841387200817621 1105 3 0.007383600645148647 1105 4 -5.547964186263243E-4 1105 5 -0.020756076275354474 1105 6 -0.06759640834660274 1105 7 0.049072881298471316 1105 8 0.020407324370097513 1105 9 0.07660117373978181 1105 10-11 0.15378528843746864 1105 12-13 0.15023299280388613 1105 14-15 0.015928060278440626 1105 16-17 -0.05349927674843258 1105 18-19 0.04232735241166807 1105 20-21 0.11768107657653104 1105 22-23 0.07899259855021512 1105 24-25 -0.05294859240979832 1105 26-27 -0.03457886535391452 1105 28-29 0.05608398757700428 1105 30-31 0.02606310216645369 1105 32-33 0.07311246815724104 1105 34-35 0.0689047200407984 1105 36-37 0.11264151722586035 1105 38-39 0.13108894704078722 1105 40-41 0.09919274524902022 1105 42-43 0.14163540583559353 1105 44-45 0.1458881432328738 1105 46-47 0.1784582808016566 1105 48-49 0.18244122943700347 1105 50-51 0.10407291125711282 1105 52-53 0.051733355324806496 1105 54-55 0.20414880738494645 1105 56-57 0.2206010501814859 1105 58-59 0.2159963712052715 1105 60-61 0.1370206588857883 1105 62-63 0.08788339859999184 1105 64-65 0.06401120789606551 1105 66-67 0.16395990206275002 1105 68-69 0.15402036695906673 1105 70-71 0.45744668518536713 1105 72-73 0.378332103426267 1105 74-75 0.2855975557041539 1105 76-77 0.20497928180389025 1105 78-79 0.24500217696719062 1105 80-81 0.3254081899629746 1105 82-83 0.2462032332743469 1105 84-85 0.25059013793604024 1105 86-87 0.17652049729035824 1105 88-89 0.43681922450846145 1105 90-91 0.41549411016434235 1105 92-93 0.5075866470873116 1105 94-95 0.5577406142824728 1105 96-97 0.557830337265294 1105 98-99 0.6050672886786117 1105 100-101 0.5053681928576026 1106 1 -0.048990964036129014 1106 2 -0.037363234136734036 1106 3 -0.019203726351989303 1106 4 -0.002498648813912041 1106 5 0.024890151736244093 1106 6 -0.00856275454442823 1106 7 0.086189027622531 1106 8 0.004815341274749585 1106 9 0.004820421994892854 1106 10-11 0.09229780684689359 1106 12-13 0.0550048074015308 1106 14-15 -0.036992749461859376 1106 16-17 -0.20619002584054158 1106 18-19 -0.12155541270693249 1106 20-21 -0.12569839085365686 1106 22-23 -0.006411128157857604 1106 24-25 0.09244013233155357 1106 26-27 -0.09490689616283277 1106 28-29 -0.09043903220912597 1106 30-31 0.07284362522283061 1106 32-33 0.15679645721678526 1106 34-35 -0.0472164482831019 1106 36-37 0.037874093447747725 1106 38-39 0.12463307020328074 1106 40-41 0.2202238560348846 1106 42-43 0.22443665721916517 1106 44-45 0.3081495067652895 1106 46-47 0.3253014645117176 1106 48-49 0.3255659378591389 1106 50-51 0.3102310356453657 1106 52-53 0.30482065030555816 1106 54-55 0.3017965172981363 1106 56-57 0.33741971848251495 1106 58-59 0.2370997475625316 1106 60-61 0.23038045910350036 1106 62-63 0.24799737695690283 1106 64-65 0.20308584268050112 1106 66-67 0.25062370250000043 1106 68-69 0.30011345233306486 1106 70-71 0.34242322174351614 1106 72-73 0.3036813107918661 1106 74-75 0.28943494902096134 1106 76-77 0.12304018612976009 1106 78-79 0.45565174383114027 1106 80-81 0.28178814221281456 1106 82-83 0.16992875216859105 1106 84-85 0.35701033936488713 1106 86-87 0.587023246126332 1106 88-89 0.5847532524450543 1106 90-91 0.6264341114449969 1106 92-93 0.5544380635839836 1106 94-95 0.6888660362127901 1106 96-97 0.6165598353397357 1106 98-99 0.6894693596818229 1106 100-101 0.9570828993587632 1107 1 0.003437670604604648 1107 2 0.05118958976900956 1107 3 -0.002422848576998149 1107 4 6.84672834196931E-5 1107 5 0.11321822129147563 1107 6 0.059925670996719305 1107 7 -0.022007170439117374 1107 8 -0.020665793016753753 1107 9 -0.11712247010975574 1107 10-11 -0.0023940104559088127 1107 12-13 -0.014238847385151132 1107 14-15 -0.0019907711553415197 1107 16-17 -0.02313892310049681 1107 18-19 0.030456958147105695 1107 20-21 0.052285398738817435 1107 22-23 0.02978797922398968 1107 24-25 0.021328987403236965 1107 26-27 0.0872785511490548 1107 28-29 0.03445376074607509 1107 30-31 0.07313705451378638 1107 32-33 0.11695055441835933 1107 34-35 0.15646932796614266 1107 36-37 0.16046744649391087 1107 38-39 0.12990894446259205 1107 40-41 0.140043521884472 1107 42-43 0.20963018398170874 1107 44-45 0.2419876399306773 1107 46-47 0.16181140403875105 1107 48-49 0.1579571368533692 1107 50-51 -0.05756989911422039 1107 52-53 -0.08765468329467296 1107 54-55 0.03282096791812705 1107 56-57 0.1630085865868267 1107 58-59 0.18402591966037818 1107 60-61 0.1501008194284239 1107 62-63 0.15508096294378504 1107 64-65 0.1973501194592373 1107 66-67 0.18833045894354683 1107 68-69 0.15944337220970084 1107 70-71 0.15825537082432106 1107 72-73 0.12454058257216616 1107 74-75 0.1133561086285475 1107 76-77 0.0363872388432398 1107 78-79 0.28792744406189996 1107 80-81 0.20689948536197988 1107 82-83 0.32417969196173857 1107 84-85 0.4331158303165772 1107 86-87 0.4419548482268496 1107 88-89 0.5989174589100159 1107 90-91 0.678824896828786 1107 92-93 0.9474155826861548 1107 94-95 0.966557450050324 1107 96-97 0.887735128905593 1107 98-99 0.9795160196214674 1107 100-101 1.022061489165683 1108 1 0.005295479546667536 1108 2 0.04775951061333927 1108 3 0.08147614059181052 1108 4 0.08595779391803404 1108 5 -0.04019920741420435 1108 6 0.060306389301317154 1108 7 0.09318541760199395 1108 8 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6 150.0 7 318.0 8 765.0 9 1458.0 10 2503.0 11 3712.0 12 5652.0 13 7777.0 14 9305.0 15 12758.0 16 16286.0 17 23153.0 18 22495.0 19 15262.0 20 14010.0 21 14803.0 22 15716.0 23 17192.0 24 20195.0 25 23599.0 26 28176.0 27 33782.0 28 41091.0 29 49844.0 30 60616.0 31 73270.0 32 90361.0 33 115152.0 34 153753.0 35 219876.0 36 342335.0 37 523788.0 38 357780.0 39 18611.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.36145052721082 20.1684736140969 14.719346051388923 22.75072980730336 2 15.33786775063388 21.69079018914908 39.68007610833621 23.29126595188083 3 19.02010517130798 25.54921224275545 31.051910029807956 24.378772556128613 4 12.60086195602192 17.591232812900053 37.90341571267219 31.90448951840584 5 12.938459970765766 38.17199559174488 34.796143889968306 14.093400547521043 6 30.442692255325998 37.230317628995834 17.736761747854317 14.59022836782385 7 26.87258789750721 33.992459383340936 21.621301086050888 17.51365163310096 8 25.122472938639795 35.34871712754397 20.55134872226537 18.97746121155086 9 25.87216745204053 17.04401147790435 21.43158684339648 35.65223422665864 10-11 24.241164436992694 27.00794821755955 27.932286015949526 20.818601329498232 12-13 25.5637266025523 24.80818781153425 28.258088437407146 21.369997148506307 14-15 22.35151818753017 25.955521017641097 27.35479893260718 24.338161862221554 16-17 21.083311693274485 29.125492574480518 28.096660309163475 21.694535423081525 18-19 20.83404335484092 27.90652120859219 30.531724046394732 20.72771139017216 20-21 21.967183845988515 26.978020378332417 30.186250491304655 20.868545284374417 22-23 21.421482451325517 27.069345243956842 30.071741183703843 21.437431121013795 24-25 21.509798053362346 27.055206397281744 30.319375993581136 21.11561955577477 26-27 21.486437740768768 27.006219982509975 30.483608724459376 21.023733552261874 28-29 20.97016721056269 27.45243443063035 30.461830658951932 21.115567699855028 30-31 21.28980424024819 27.278561819612623 30.2489961971521 21.182637742987083 32-33 21.053057477721097 27.364706043539655 30.219539325352006 21.36269715338724 34-35 21.590978187981253 27.152619043744792 30.07556144388045 21.18084132439351 36-37 21.59251647571863 26.960074138057294 30.234276603644467 21.213132782579613 38-39 21.34565375918005 27.281411926111698 30.277070416890222 21.095863897818028 40-41 21.539053742409465 27.21880012365798 30.1026686659753 21.139477467957253 42-43 21.163066720673708 27.48475913916432 29.714540539105577 21.63763360105639 44-45 21.435866981593158 27.46053803105578 29.522046317143364 21.5815486702077 46-47 21.54831936505993 27.60952744070504 29.08979549283147 21.752357701403565 48-49 21.713656432933824 27.62681842003405 28.982369846168606 21.677155300863525 50-51 21.425745601021408 27.940134415197132 29.04150070281756 21.5926192809639 52-53 21.65242775145593 28.15171402172787 28.540069480540055 21.655788746276148 54-55 21.26355850999607 27.765606361999268 28.80629452247756 22.1645406055271 56-57 21.558255673658962 28.12103691613954 28.47627480178694 21.84443260841456 58-59 21.75191648043658 27.723602942545938 28.64165535442445 21.88282522259303 60-61 21.576053913802994 27.639558482261368 29.031953519040236 21.7524340848954 62-63 21.493489228208194 27.704700865862215 28.901036183133087 21.900773722796497 64-65 21.30527664251375 27.899672226183842 28.708756127161084 22.086295004141327 66-67 21.226458920723 27.9257732135326 28.4738037588657 22.373964106878695 68-69 21.191749011635586 27.908538051307392 28.6134682165939 22.28624472046312 70-71 21.313138535496815 27.81912538779886 28.39216430084029 22.475571775864033 72-73 21.359657272721826 27.535388920619724 28.487299721101657 22.617654085556797 74-75 21.230119822676482 28.056856900400668 28.108920208698514 22.60410306822434 76-77 21.32409423740711 27.583796713034275 28.357037818254593 22.73507123130402 78-79 21.226528397133848 27.66081433161995 28.394393575776 22.718263695470203 80-81 21.14622585162867 27.85444335170253 28.29235836587654 22.70697243079226 82-83 21.195246825465468 27.74291429506024 28.26776467727171 22.794074202202587 84-85 21.12813185914536 27.568752278576913 28.11993508343739 23.183180778840335 86-87 21.11261676495558 27.532466196714108 28.252287301038344 23.102629737291974 88-89 21.182831536440304 27.346568844584162 28.116659714188646 23.353939904786888 90-91 21.016575625809182 27.319951444876008 28.256838380555944 23.40663454875887 92-93 21.08174367554968 27.518369870415455 28.13182635174074 23.268060102294125 94-95 21.00562340404762 27.28241528072492 28.066412170598 23.645549144629467 96-97 20.76183279154228 27.18861286148096 28.181241149289214 23.86831319768755 98-99 20.83016143907094 27.143544021325404 27.844022438600835 24.18227210100282 100-101 20.991245530605106 26.46423392291597 28.64679288938593 23.897727657092997 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1772.0 1 1174.0 2 678.0 3 793.5 4 704.0 5 700.5 6 1208.0 7 2273.0 8 3365.5 9 3860.5 10 3482.0 11 2984.0 12 3071.5 13 3276.5 14 3533.5 15 3759.5 16 3866.5 17 4146.5 18 4512.5 19 4910.5 20 5487.5 21 6255.5 22 7319.5 23 8837.0 24 10858.0 25 13261.0 26 16389.5 27 20025.0 28 23566.5 29 27197.5 30 30980.0 31 35292.5 32 40016.5 33 44522.0 34 49505.0 35 54426.0 36 59630.5 37 64357.5 38 66873.5 39 68273.0 40 70425.5 41 73385.0 42 75887.0 43 79037.5 44 82267.0 45 84114.0 46 85453.0 47 91398.0 48 108104.0 49 108470.0 50 92315.0 51 84387.0 52 78294.5 53 73182.5 54 68318.5 55 62049.0 56 54936.0 57 48058.0 58 42365.5 59 36848.5 60 31069.0 61 25006.0 62 19613.0 63 14989.0 64 11128.5 65 8388.5 66 5954.5 67 4390.5 68 3169.5 69 2044.0 70 1343.0 71 900.0 72 669.5 73 475.5 74 334.0 75 240.0 76 168.5 77 77.5 78 35.5 79 26.0 80 12.0 81 8.0 82 5.5 83 3.5 84 1.5 85 1.5 86 4.0 87 3.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39899504114114553 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.777120231133688E-4 16-17 0.0024618751867814 18-19 3.4252176511741217E-4 20-21 0.0 22-23 0.0 24-25 4.281522063967652E-5 26-27 2.140761031983826E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012844566191902957 36-37 0.02204983862943341 38-39 0.010917881263117513 40-41 0.006165391772113419 42-43 0.0051378264767611825 44-45 0.0019909077597449584 46-47 0.0011774185675911043 48-49 0.0031683263273360627 50-51 8.13489192153854E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 9.205272437530453E-4 60-61 0.001626978384307708 62-63 0.0011560109572712662 64-65 5.780054786356331E-4 66-67 0.0031255111066963858 68-69 0.0028900273931781652 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09113219713155148 78-79 0.003553663313093151 80-81 2.1407610319838262E-4 82-83 0.0 84-85 1.9266849287854435E-4 86-87 0.09380814842153126 88-89 0.4413392943537856 90-91 0.3934718776786273 92-93 0.0 94-95 9.419348540728834E-4 96-97 2.140761031983826E-5 98-99 0.0 100-101 4.709674270364417E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2335618.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.141505912022446 #Duplication Level Percentage of deduplicated Percentage of total 1 70.88030983836973 36.24925784644968 2 15.34956926968902 15.70000175105598 3 5.749830629343624 8.82164991371114 4 2.7944418020134623 5.716478477538964 5 1.5622325089473725 3.994746154614285 6 0.9445643057077611 2.89838646147833 7 0.6056564057703271 2.168192645945021 8 0.3975204598854203 1.6263835959504078 9 0.28155054367647997 1.2959026914567449 >10 1.317500204406051 11.597487323484735 >50 0.07440953492906385 2.612910705634449 >100 0.03789318458180377 3.4643096096813477 >500 0.0023443843525295877 0.8144319589993846 >1k 0.0018420162769869444 1.6586664754885634 >5k 2.5118403777094696E-4 0.9138594568328406 >10k+ 8.372801259031565E-5 0.4673349316780819 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10914 0.46728531806142953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9141 0.3913739318672831 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6312 0.2702496726776382 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5889 0.25213883434705503 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3256 0.13940635840278676 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3013 0.12900225978734536 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2872 0.12296531367715097 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2468 0.10566796453872165 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2844566191902958E-4 2 0.0 0.0 0.0 0.0 1.2844566191902958E-4 3 0.0 0.0 0.0 0.0 1.2844566191902958E-4 4 0.0 0.0 0.0 0.0 1.2844566191902958E-4 5 0.0 0.0 0.0 0.0 1.2844566191902958E-4 6 0.0 0.0 0.0 0.0 1.2844566191902958E-4 7 0.0 0.0 0.0 0.0 1.2844566191902958E-4 8 0.0 0.0 0.0 0.0 1.2844566191902958E-4 9 0.0 0.0 0.0 0.0 1.2844566191902958E-4 10-11 0.0 0.0 0.0 4.281522063967652E-5 1.2844566191902958E-4 12-13 0.0 0.0 0.0 6.422283095951478E-5 3.211141547975739E-4 14-15 0.0 0.0 0.0 1.0703805159919131E-4 3.6392937543725044E-4 16-17 0.0 0.0 0.0 1.9266849287854435E-4 4.2815220639676524E-4 18-19 0.0 0.0 0.0 2.5689132383805916E-4 4.709674270364417E-4 20-21 0.0 0.0 0.0 3.6392937543725044E-4 5.994130889554713E-4 22-23 0.0 0.0 0.0 5.565978683157947E-4 5.994130889554713E-4 24-25 0.0 0.0 0.0 0.0010061576850323984 5.994130889554713E-4 26-27 0.0 0.0 0.0 0.0021835762526235024 5.994130889554713E-4 28-29 0.0 0.0 0.0 0.006829027692028406 5.994130889554713E-4 30-31 0.0 0.0 0.0 0.020165968921287643 5.994130889554713E-4 32-33 0.0 0.0 0.0 0.04617621545989113 6.422283095951478E-4 34-35 0.0 0.0 0.0 0.08700052833982269 6.422283095951478E-4 36-37 0.0 0.0 0.0 0.15255063113916745 6.422283095951478E-4 38-39 0.0 0.0 0.0 0.2420344422760914 6.422283095951478E-4 40-41 0.0 0.0 0.0 0.34397748261916117 6.422283095951478E-4 42-43 0.0 0.0 0.0 0.45289940392649825 6.422283095951478E-4 44-45 0.0 0.0 0.0 0.586226000998451 6.636359199149861E-4 46-47 0.0 0.0 0.0 0.7440214966659788 6.850435302348243E-4 48-49 0.0 0.0 0.0 0.9160744608065189 7.278587508745009E-4 50-51 0.0 0.0 0.0 1.0946567460946097 7.706739715141774E-4 52-53 0.0 0.0 0.0 1.270498857261761 8.563044127935305E-4 54-55 0.0 0.0 0.0 1.4693541495227387 8.563044127935305E-4 56-57 0.0 0.0 0.0 1.7112173309162713 8.563044127935305E-4 58-59 0.0 0.0 0.0 1.9801397317540796 8.563044127935305E-4 60-61 0.0 0.0 0.0 2.276014313984564 8.563044127935305E-4 62-63 0.0 0.0 0.0 2.5678214502542795 9.419348540728834E-4 64-65 0.0 0.0 0.0 2.874935027902679 9.419348540728834E-4 66-67 0.0 0.0 0.0 3.2316286310518243 9.419348540728834E-4 68-69 0.0 0.0 0.0 3.628953878588023 9.419348540728834E-4 70-71 0.0 0.0 0.0 4.072862086180189 9.633424643927218E-4 72-73 0.0 0.0 0.0 4.529165300147541 0.0010917881263117512 74-75 0.0 0.0 0.0 5.00584855913938 0.0012844566191902956 76-77 0.0 0.0 0.0 5.509483999523894 0.0012844566191902956 78-79 0.0 0.0 0.0 6.088238744520722 0.0012844566191902956 80-81 0.0 0.0 0.0 6.7106650145700195 0.0012844566191902956 82-83 0.0 0.0 0.0 7.342446410329087 0.0013272718398299722 84-85 0.0 0.0 0.0 7.997947438322534 0.0013272718398299722 86-87 0.0 0.0 0.0 8.692495947539367 0.0013486794501498104 88-89 0.0 0.0 0.0 9.430030938278435 0.0013700870604696487 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 340 0.0 25.121656 2 TTTGCGT 380 0.0 23.726007 3 GTACATG 9320 0.0 23.619942 1 TACATGG 9225 0.0 22.890133 2 ACATGGG 9305 0.0 21.418427 3 GAGTACT 9315 0.0 20.913729 12-13 AGTACTT 9735 0.0 20.840174 12-13 CATGGGG 3475 0.0 19.936674 4 GTACTTT 10285 0.0 18.849026 14-15 GGTATCA 16955 0.0 18.772903 1 GTATCAA 21020 0.0 18.724064 1 GTATAGG 575 0.0 18.230879 1 TACTTTT 11805 0.0 17.065258 14-15 TTGCGTT 505 0.0 16.91359 4 AGAGTAC 17955 0.0 16.876276 10-11 CATGGGA 6345 0.0 16.453024 4 ATCAACG 23875 0.0 16.416906 3 CAACGCA 23915 0.0 16.40929 5 TCAACGC 24120 0.0 16.25015 4 TATCAAC 24350 0.0 16.155123 2 >>END_MODULE