FastQCFastQC Report
Fri 27 May 2016
SRR939002_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939002_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346499
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112180.4780739305663459No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87410.3725124110430049No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59760.25467728731186334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54820.23362464676098307No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35450.15107613512726834No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31870.13581936323007168No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28660.12213940854012723No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25760.10978057096977241No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG93000.025.1363281
TACATGG94550.023.929042
ACATGGG93150.023.6789263
GAGTACT87550.022.97568712-13
GTACCGT1353.8926298E-421.0339496
GTACTTT96300.020.54425414-15
CATGGGA62800.020.5006794
AGTACTT92950.020.08784712-13
TTTGCGT4050.019.8679393
GTATAGG6150.019.275371
GTATCAA216050.019.0723131
CATGGGG33750.018.9329784
ATGGGAT28000.017.5806225
GGTATCA173850.017.1831871
ACTTTTT118900.017.05817416-17
TAGACCG1952.3388519E-416.9911335
CGGGTCG1952.3411388E-416.9889586
CAACGCA243800.016.948825
ATCAACG243250.016.9482253
TCAACGC244750.016.8636954
Produced by FastQC (version 0.11.5)