##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939002_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2346499 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.866582512926705 33.0 31.0 34.0 30.0 34.0 2 31.92688682160103 33.0 31.0 34.0 30.0 34.0 3 31.97287703936801 34.0 31.0 34.0 30.0 34.0 4 35.516405078374206 37.0 35.0 37.0 33.0 37.0 5 35.55744835177855 37.0 35.0 37.0 33.0 37.0 6 35.640728591829784 37.0 35.0 37.0 33.0 37.0 7 35.55849203430302 37.0 35.0 37.0 33.0 37.0 8 35.54531708728621 37.0 35.0 37.0 33.0 37.0 9 37.165976205402174 39.0 37.0 39.0 33.0 39.0 10-11 37.05865014219056 39.0 37.0 39.0 33.0 39.0 12-13 36.94200082761595 39.0 37.0 39.0 33.0 39.0 14-15 38.16284622324578 40.0 38.0 41.0 33.0 41.0 16-17 38.098627785479565 40.0 38.0 41.0 33.0 41.0 18-19 38.05930835683288 40.0 38.0 41.0 33.0 41.0 20-21 37.955838890193434 40.0 38.0 41.0 33.0 41.0 22-23 38.0227986459828 40.0 38.0 41.0 33.0 41.0 24-25 37.96047515894956 40.0 38.0 41.0 33.0 41.0 26-27 37.87807687111736 40.0 38.0 41.0 33.0 41.0 28-29 37.75480151493779 40.0 38.0 41.0 32.0 41.0 30-31 37.565680616100835 40.0 37.0 41.0 32.0 41.0 32-33 37.36528547423204 40.0 37.0 41.0 31.5 41.0 34-35 37.050892840781096 40.0 36.0 41.0 30.5 41.0 36-37 36.78595239120068 40.0 36.0 41.0 30.0 41.0 38-39 36.57741533237389 39.0 36.0 41.0 30.0 41.0 40-41 36.43107454978673 39.0 35.0 41.0 30.0 41.0 42-43 36.22057030495219 39.0 35.0 41.0 30.0 41.0 44-45 36.12308081102954 39.0 35.0 41.0 29.5 41.0 46-47 35.86846403940509 39.0 35.0 41.0 27.5 41.0 48-49 35.667489097587506 39.0 35.0 41.0 27.0 41.0 50-51 34.49990326013351 38.0 34.0 40.0 25.0 40.5 52-53 34.6407790925971 38.5 34.5 39.5 25.0 40.5 54-55 35.15031350961581 39.0 35.0 40.5 26.0 41.0 56-57 35.06446646685126 39.0 35.0 41.0 25.5 41.0 58-59 34.88007857663694 39.0 35.0 41.0 25.0 41.0 60-61 34.623890314890396 38.0 34.0 40.0 24.5 41.0 62-63 34.28550299829661 38.0 34.0 40.0 23.5 41.0 64-65 33.88929230312904 37.5 34.0 40.0 22.0 41.0 66-67 33.51759408378184 37.0 33.5 40.0 22.0 41.0 68-69 33.13079570884113 36.0 33.0 39.0 20.5 41.0 70-71 32.71552022822085 36.0 32.5 39.0 19.5 40.5 72-73 32.282890169567516 35.0 32.0 38.5 18.0 40.0 74-75 31.812104756916582 35.0 32.0 37.5 16.0 39.5 76-77 31.3895978647338 35.0 31.0 37.0 12.0 39.0 78-79 30.9524406360284 35.0 31.0 36.5 7.5 39.0 80-81 30.50310973923279 35.0 31.0 36.0 6.5 38.0 82-83 30.11675990486252 34.0 30.5 35.5 2.0 37.0 84-85 29.752973685477812 34.0 30.0 35.0 2.0 37.0 86-87 29.35062661437316 34.0 30.0 35.0 2.0 36.0 88-89 28.930863384130998 34.0 29.0 35.0 2.0 36.0 90-91 28.499163008379718 34.0 29.0 35.0 2.0 36.0 92-93 28.08437058784172 34.0 29.0 35.0 2.0 35.0 94-95 27.612974904314896 34.0 27.5 35.0 2.0 35.0 96-97 27.036471568920334 34.0 26.5 35.0 2.0 35.0 98-99 26.346446770273502 33.0 25.0 35.0 2.0 35.0 100-101 23.760459092460724 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2844267492958217 1101 2 0.3753315627196123 1101 3 0.2585366763675516 1101 4 0.21203774890813776 1101 5 0.11277833615339006 1101 6 0.10321691600515948 1101 7 0.1527005955519769 1101 8 0.28952576761598436 1101 9 0.309553376726619 1101 10-11 0.33882513938906555 1101 12-13 0.3606317071971077 1101 14-15 0.11451456246596337 1101 16-17 0.35607375407556674 1101 18-19 0.3765082993801414 1101 20-21 0.5379067746663111 1101 22-23 0.5064520194345761 1101 24-25 0.5526325083312642 1101 26-27 0.5407345431690729 1101 28-29 0.47898258711909847 1101 30-31 0.4705729898540838 1101 32-33 0.4561635330283167 1101 34-35 0.3869207760856668 1101 36-37 0.3922222390657879 1101 38-39 0.4148998487655433 1101 40-41 0.3316226848350112 1101 42-43 0.4042296070985927 1101 44-45 0.46559696914217596 1101 46-47 0.5067480642014459 1101 48-49 0.5838849095762839 1101 50-51 0.5170614687623072 1101 52-53 0.403331698913874 1101 54-55 0.34262121359243025 1101 56-57 0.42499058300666803 1101 58-59 0.33093356531342266 1101 60-61 0.4051531838602074 1101 62-63 0.34475949100121994 1101 64-65 0.3354161627136776 1101 66-67 0.20985604559520965 1101 68-69 0.2113022575459098 1101 70-71 0.2394657673412226 1101 72-73 0.11216064459622288 1101 74-75 0.042515966805812155 1101 76-77 0.12060670463412748 1101 78-79 0.10120370409557822 1101 80-81 0.0925180321128991 1101 82-83 -0.04466739100527661 1101 84-85 -0.17396340519630726 1101 86-87 -0.16972514295527574 1101 88-89 -0.17856394010809495 1101 90-91 -0.17373767927023565 1101 92-93 -0.2601342898330721 1101 94-95 -0.131794696097419 1101 96-97 -0.1857964535298997 1101 98-99 -0.3740447053884175 1101 100-101 0.0546327689704178 1102 1 0.22050792230057326 1102 2 0.16794907140360138 1102 3 0.2090515083391189 1102 4 0.12515645899605232 1102 5 0.24762655060075645 1102 6 0.3389209852598256 1102 7 0.2976656914703071 1102 8 0.12105505523130944 1102 9 0.211926699093425 1102 10-11 0.2754352404932163 1102 12-13 0.40341798144826413 1102 14-15 0.45902134024633057 1102 16-17 0.5124411948653744 1102 18-19 0.5553157411010545 1102 20-21 0.5570910873572288 1102 22-23 0.5174851492146502 1102 24-25 0.5795722777755117 1102 26-27 0.5493913761700426 1102 28-29 0.6506456198298665 1102 30-31 0.5303864812709591 1102 32-33 0.46432700155515505 1102 34-35 0.4655099218085894 1102 36-37 0.464251512455661 1102 38-39 0.40629539128541126 1102 40-41 0.3232548871157377 1102 42-43 0.315661954737557 1102 44-45 0.5031372853609923 1102 46-47 0.4836375272812319 1102 48-49 0.5507072802604682 1102 50-51 0.5319535751893625 1102 52-53 0.46434077475103663 1102 54-55 0.5764294183147669 1102 56-57 0.6166041628799874 1102 58-59 0.6219759287038329 1102 60-61 0.4478159553399905 1102 62-63 0.40371686896625647 1102 64-65 0.44004111518773925 1102 66-67 0.4142227414887074 1102 68-69 0.3619956835774474 1102 70-71 0.2981347566359318 1102 72-73 0.4089255185975347 1102 74-75 0.387081586220706 1102 76-77 0.24462353204838294 1102 78-79 0.18997051275809795 1102 80-81 0.1235760693628869 1102 82-83 0.14562063556269322 1102 84-85 -0.044614620437712915 1102 86-87 0.059137131409684685 1102 88-89 0.032199035442236124 1102 90-91 -0.07553099123952833 1102 92-93 0.07487247372184314 1102 94-95 0.04563093712909705 1102 96-97 -0.02424318956503768 1102 98-99 -0.05435487192964317 1102 100-101 0.175751484231224 1103 1 -0.005155930948355092 1103 2 0.2321664145905622 1103 3 0.2300754723913805 1103 4 0.18177149078034205 1103 5 0.1979921449019173 1103 6 0.2508100085971918 1103 7 0.18270182588767625 1103 8 0.097980196318872 1103 9 0.22768675522844006 1103 10-11 0.32764909857562685 1103 12-13 0.3321260510236854 1103 14-15 0.19391672044106656 1103 16-17 0.2324500867987922 1103 18-19 0.2395116836459934 1103 20-21 0.3486381671029477 1103 22-23 0.4076522708949639 1103 24-25 0.4589767849107247 1103 26-27 0.3621761763807143 1103 28-29 0.32483542885747596 1103 30-31 0.45026886940927113 1103 32-33 0.5063943052409314 1103 34-35 0.3152560391921142 1103 36-37 0.34487980630447623 1103 38-39 0.31223444445700466 1103 40-41 0.409687063414367 1103 42-43 0.2499731090721795 1103 44-45 0.3659450445937651 1103 46-47 0.35062499523860424 1103 48-49 0.2311501935478688 1103 50-51 0.2470548198933713 1103 52-53 0.2800385790184947 1103 54-55 0.37341457786956056 1103 56-57 0.3081432423857038 1103 58-59 0.29922166274251794 1103 60-61 0.28743281051231406 1103 62-63 0.1558102651014721 1103 64-65 0.1413109032813793 1103 66-67 0.12025600581997509 1103 68-69 0.011143283005431215 1103 70-71 0.044645901970284285 1103 72-73 0.03929401298569957 1103 74-75 0.018474069661873926 1103 76-77 -0.04262071027888936 1103 78-79 0.0536687153708435 1103 80-81 0.06954319837675627 1103 82-83 0.20072833046020833 1103 84-85 0.20253216896596982 1103 86-87 0.2663075131878081 1103 88-89 0.22712071642599696 1103 90-91 0.1752763246466138 1103 92-93 0.09102816672596603 1103 94-95 0.08927724651838176 1103 96-97 0.0014854989622854475 1103 98-99 -0.20622796978924285 1103 100-101 -0.06253795611865343 1104 1 0.24200729462257087 1104 2 0.13069093493291817 1104 3 0.14240662884292377 1104 4 0.21613242048463377 1104 5 0.17636901587096787 1104 6 0.23990991530225614 1104 7 0.26314132615133445 1104 8 0.2543323644120079 1104 9 0.3468168813794179 1104 10-11 0.20640736328986264 1104 12-13 0.2800517598623671 1104 14-15 0.26643852306143856 1104 16-17 0.22548247636598973 1104 18-19 0.3482057573895574 1104 20-21 0.3265475493282395 1104 22-23 0.3367706337716925 1104 24-25 0.25691486546887177 1104 26-27 0.16617102487786184 1104 28-29 0.20253570874226767 1104 30-31 0.35451774378561396 1104 32-33 0.31616563124588026 1104 34-35 0.2310893049204239 1104 36-37 0.2567312037003333 1104 38-39 0.2324580480752303 1104 40-41 0.2842660193223665 1104 42-43 0.207253663162966 1104 44-45 0.3914288599509277 1104 46-47 0.5004322195115165 1104 48-49 0.5956900061467394 1104 50-51 0.3191733247406461 1104 52-53 0.37955060595234613 1104 54-55 0.3803847234907778 1104 56-57 0.4748296497195028 1104 58-59 0.37278776017488013 1104 60-61 0.4077248642177409 1104 62-63 0.3505411294993195 1104 64-65 0.40852037068899705 1104 66-67 0.45168443238063105 1104 68-69 0.4839026834840823 1104 70-71 0.4399132122367888 1104 72-73 0.48955381909448903 1104 74-75 0.44062117143448276 1104 76-77 0.4003632064858067 1104 78-79 0.24255799862237737 1104 80-81 0.4255808002913284 1104 82-83 0.4083307520721817 1104 84-85 0.4053355803154979 1104 86-87 0.4381194630519225 1104 88-89 0.3733751276538406 1104 90-91 0.5802492488343383 1104 92-93 0.49516245058594066 1104 94-95 0.4312713243182422 1104 96-97 0.3243668927074701 1104 98-99 0.2958653859889573 1104 100-101 0.5945387688235435 1105 1 0.16186030978469645 1105 2 0.09946997430315108 1105 3 0.19266853806745488 1105 4 0.22430832878188056 1105 5 0.12336963735048556 1105 6 0.10293819755260358 1105 7 0.11317831897965647 1105 8 0.1343562371172169 1105 9 0.18110597786657934 1105 10-11 0.08097573096898003 1105 12-13 0.1735489138807722 1105 14-15 0.20464165808032675 1105 16-17 0.2918744425101494 1105 18-19 0.26025831680004075 1105 20-21 0.22343571260964978 1105 22-23 0.2004470303742707 1105 24-25 0.20298020960083107 1105 26-27 0.16661424828495086 1105 28-29 0.2354174993924616 1105 30-31 0.21485926157669866 1105 32-33 0.13594041890704744 1105 34-35 0.2538083271259808 1105 36-37 0.25259126229697415 1105 38-39 0.37146506191547246 1105 40-41 0.36335826463907495 1105 42-43 0.2212857828027026 1105 44-45 0.0654374028280813 1105 46-47 0.06945274806503932 1105 48-49 0.11312595922997559 1105 50-51 0.17661036294684607 1105 52-53 0.11118598490687503 1105 54-55 0.07276252384787796 1105 56-57 -0.030073034480125216 1105 58-59 -0.03440709831522071 1105 60-61 0.09558756656851841 1105 62-63 0.015732357759858928 1105 64-65 0.05432163535048318 1105 66-67 0.008171889345639727 1105 68-69 -0.09754565973045004 1105 70-71 -0.28126740154822016 1105 72-73 -0.1307459707862506 1105 74-75 -0.1811312035050996 1105 76-77 -0.15155489632466512 1105 78-79 -0.15046860661975714 1105 80-81 -0.18731529425247473 1105 82-83 -0.1900748077032972 1105 84-85 -0.041920538298303 1105 86-87 -0.01634986249483461 1105 88-89 -0.12424519768575593 1105 90-91 -0.13325382403659702 1105 92-93 0.008539846488123004 1105 94-95 -0.14391546480290884 1105 96-97 -0.196081164429188 1105 98-99 0.07527034401443089 1105 100-101 0.3161682289255445 1106 1 0.16139261886651113 1106 2 0.1714899141143782 1106 3 0.1926144170528694 1106 4 0.06591221959786964 1106 5 0.054758545529821845 1106 6 0.05191585352685024 1106 7 0.11833106632918344 1106 8 0.1806397916613065 1106 9 0.21330235606500736 1106 10-11 0.1636409251819515 1106 12-13 0.15869996970612021 1106 14-15 0.10664159445418875 1106 16-17 0.13775583329995555 1106 18-19 0.1990876036014555 1106 20-21 0.2795005251433764 1106 22-23 0.32104416944821423 1106 24-25 0.27035670214783636 1106 26-27 0.3190309552675643 1106 28-29 0.27740609557093165 1106 30-31 0.1436614180372544 1106 32-33 0.24562131054203462 1106 34-35 0.3226829436718077 1106 36-37 0.2476568418360472 1106 38-39 0.2870900122075568 1106 40-41 0.37097710682181173 1106 42-43 0.39855237671313404 1106 44-45 0.5157201705806429 1106 46-47 0.49407226181391906 1106 48-49 0.5953300385190019 1106 50-51 0.39917799190713765 1106 52-53 0.40428221905416706 1106 54-55 0.29030970127208633 1106 56-57 0.3293899182305253 1106 58-59 0.4547759730969787 1106 60-61 0.4603861917783547 1106 62-63 0.27465349898001534 1106 64-65 0.12633226401941755 1106 66-67 0.26179023803783963 1106 68-69 0.29907544312003864 1106 70-71 0.3320018685396917 1106 72-73 0.2723631554134798 1106 74-75 0.3453279591826721 1106 76-77 0.40079477043343203 1106 78-79 0.3680054756216826 1106 80-81 0.3504698061352194 1106 82-83 0.35092341270463123 1106 84-85 0.4883212759467348 1106 86-87 0.624962919336955 1106 88-89 0.6927540966072527 1106 90-91 0.6157949845833492 1106 92-93 0.6147107284511613 1106 94-95 0.6907910033760665 1106 96-97 0.6203209558731011 1106 98-99 0.5631004870529708 1106 100-101 1.0492751980439756 1107 1 -0.0638866742917763 1107 2 -0.1210882133611797 1107 3 -0.03654145918914864 1107 4 -0.039363724327827754 1107 5 -0.004599688502800348 1107 6 -0.07380634875489278 1107 7 -0.04386051476785724 1107 8 -0.043161153306229494 1107 9 -0.09572407541025285 1107 10-11 0.03123965408673257 1107 12-13 -0.009273838680520896 1107 14-15 0.06424778039459511 1107 16-17 0.06531264302532946 1107 18-19 0.08162220065555204 1107 20-21 0.07603262909326958 1107 22-23 0.03269502424188886 1107 24-25 0.07561879976221775 1107 26-27 0.17870646379302713 1107 28-29 0.195740128901555 1107 30-31 0.12881773060131252 1107 32-33 0.12308279964847912 1107 34-35 -0.030078531242651252 1107 36-37 0.015718356330602035 1107 38-39 0.16064764810256804 1107 40-41 0.08063906670284382 1107 42-43 0.004219320498073387 1107 44-45 0.21869634013784633 1107 46-47 0.2888784739490049 1107 48-49 0.19298282949174705 1107 50-51 0.334428050336264 1107 52-53 0.17309049334863857 1107 54-55 0.26035884319447433 1107 56-57 0.245033811075821 1107 58-59 0.23483845625718658 1107 60-61 0.13864410304405084 1107 62-63 0.0911028784213741 1107 64-65 0.3219565415770873 1107 66-67 0.3061124534169224 1107 68-69 0.29962276610569916 1107 70-71 0.47906717070135585 1107 72-73 0.39481025376627343 1107 74-75 0.5721739403820258 1107 76-77 0.4846540619516766 1107 78-79 0.45519393121641016 1107 80-81 0.5240369673706198 1107 82-83 0.5516051466929852 1107 84-85 0.4318863227870189 1107 86-87 0.4362304944061428 1107 88-89 0.5044091187373461 1107 90-91 0.5284804526587656 1107 92-93 0.41457814185842423 1107 94-95 0.528440410548459 1107 96-97 0.4819019165612879 1107 98-99 0.4614482053786695 1107 100-101 0.4327122058733863 1108 1 0.11022180376899016 1108 2 0.1779369998462066 1108 3 0.1358001875626762 1108 4 0.1434215273638415 1108 5 0.11473392165410701 1108 6 0.15165987150955829 1108 7 0.18153175682123646 1108 8 0.17160670689592905 1108 9 0.23762842263214168 1108 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90-91 -2.927901747320849 2316 92-93 -3.055804171440613 2316 94-95 -3.1670530613566044 2316 96-97 -3.4180197168303295 2316 98-99 -3.8408651513810987 2316 100-101 -4.5454602100830215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6899.0 3 1642.0 4 1565.0 5 1857.0 6 2044.0 7 2632.0 8 3416.0 9 4010.0 10 5187.0 11 6774.0 12 9683.0 13 14595.0 14 14095.0 15 13560.0 16 17437.0 17 23452.0 18 19966.0 19 13685.0 20 13353.0 21 14000.0 22 15518.0 23 17131.0 24 19330.0 25 22535.0 26 26233.0 27 30534.0 28 36047.0 29 42971.0 30 52029.0 31 63043.0 32 79220.0 33 100910.0 34 134616.0 35 192950.0 36 294007.0 37 468576.0 38 474921.0 39 86033.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52588504851083 19.486602840344013 14.144620856513862 22.842891254631297 2 15.033325115441476 21.25360101481012 40.45953939427028 23.253534475478123 3 19.079462034900335 25.387456865112036 30.811895631699503 24.721185468288127 4 12.360775748061057 17.002935078453625 38.37700400160406 32.25928517188127 5 13.033653027782744 37.86939384618403 34.817613754895994 14.279339371137226 6 30.443685628349204 36.80902572221644 18.108213468635 14.639075180799354 7 26.920095941346617 33.889280137044445 21.862700476763486 17.327923444845457 8 25.01043964223271 34.970050276634524 20.910123531145537 19.109386549987224 9 26.026325005513346 17.102594323022842 21.62985201195575 35.24122865950806 10-11 24.209207421301425 26.74118963048112 28.212248623621356 20.837354324596095 12-13 25.68481865957959 24.692053678581566 28.30866741328076 21.314460248558085 14-15 22.473617437702682 25.793225894797374 27.499507286612445 24.2336493808875 16-17 21.206870848798204 28.949340062484364 28.13265845612989 21.711130632587544 18-19 20.90644023257699 27.814450317169833 30.49601778028044 20.78309166997274 20-21 22.09563097373636 26.892814559012677 30.105434247039923 20.90612022021104 22-23 21.546935632169195 26.98422856947813 29.98502428753368 21.48381151081899 24-25 21.54337927211699 26.979288502872436 30.268860724708247 21.208471500302327 26-27 21.525754226263718 26.913134974373897 30.407677012765365 21.15343378659702 28-29 21.02023234965692 27.356127670704716 30.425734002439814 21.197905977198552 30-31 21.318294232681396 27.26515022709901 30.142164342842175 21.274391197377422 32-33 21.034582723335866 27.28244288813171 30.21576364998877 21.467210738543656 34-35 21.651920167837147 27.12806052952209 29.958579750023954 21.26143955261681 36-37 21.64329207127094 26.900818439494977 30.122898815637626 21.332990673596452 38-39 21.37593938395143 27.230931740724778 30.21677275175233 21.176356123571463 40-41 21.578318551936547 27.138571658407805 30.062638217431886 21.220471572223758 42-43 21.149939571601223 27.368771803509134 29.695715155099556 21.785573469790087 44-45 21.420407015510364 27.377323837976526 29.48463034314754 21.717638803365567 46-47 21.601834802615564 27.550156186243953 29.09682432733619 21.751184683804293 48-49 21.67326909461389 27.571940599369416 29.006840645519606 21.747949660497092 50-51 21.506462074495346 27.837492527767555 29.10335139672073 21.552694001016373 52-53 21.604284929186097 28.052212554024592 28.701343573677924 21.642158943111387 54-55 21.251560512523213 27.703514943950143 28.95576127828613 22.089163265240515 56-57 21.599613297442787 27.995439618114084 28.542046939536803 21.862900144906327 58-59 21.787291407288283 27.597238639253597 28.724716432607472 21.890753520850648 60-61 21.654981490947193 27.453828013029035 29.15455803225652 21.736632463767247 62-63 21.58002009682392 27.547992471298592 29.0392890978909 21.832698333986585 64-65 21.349438005700545 27.748682906249677 28.95947021275866 21.942408875291118 66-67 21.32904165285585 27.748188442306944 28.686909574123682 22.235860330713532 68-69 21.22830729701556 27.711386870766358 28.88592632360275 22.17437950861533 70-71 21.336862556262535 27.62295858708735 28.667125185505032 22.373053671145087 72-73 21.463170452754916 27.26991112973815 28.85386196007645 22.413056457430493 74-75 21.280129205247956 27.87463026488019 28.515897484707896 22.329343045163956 76-77 21.427097106620973 27.428640750791487 28.673072582783334 22.47118955980421 78-79 21.398764077062825 27.48473525028961 28.770287106295456 22.34621356635211 80-81 21.342833474541226 27.633462884609365 28.643088591559497 22.38061504928991 82-83 21.409679054973534 27.48262963961458 28.685750239698333 22.42194106571356 84-85 21.380472426836594 27.300221204276287 28.59240481261291 22.72690155627421 86-87 21.31914892705086 27.287066212254764 28.77239136943126 22.621393491263113 88-89 21.512630293843806 27.120130966853623 28.611233519832783 22.756005219469788 90-91 21.386723041460726 27.07479942524806 28.806493676592492 22.731983856698726 92-93 21.469052517122538 27.205778315538353 28.756711169117604 22.568457998221504 94-95 21.460927414116373 26.96666356531049 28.65185761945331 22.92055140111983 96-97 21.298810211077615 26.806254618387975 28.79042235030742 23.10451282022699 98-99 21.407624884904266 26.727700904365758 28.54824481010677 23.316429400623207 100-101 21.641280487099152 26.258288748734905 29.03422922620627 23.06620153795967 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3818.0 1 2300.0 2 904.5 3 1006.5 4 876.5 5 876.0 6 1390.5 7 2526.0 8 3818.0 9 4425.0 10 4001.5 11 3576.5 12 3747.0 13 4043.0 14 4322.0 15 4545.0 16 4801.0 17 4975.5 18 5191.5 19 5524.5 20 5856.5 21 6563.5 22 7548.5 23 8949.5 24 10927.5 25 13251.0 26 16463.5 27 20131.0 28 23801.0 29 27329.0 30 30919.5 31 35278.5 32 39833.0 33 44480.5 34 49747.0 35 54804.5 36 59831.0 37 64425.5 38 66903.5 39 68341.5 40 70598.0 41 73498.0 42 75857.5 43 78737.5 44 82173.0 45 84538.0 46 86248.0 47 91661.5 48 108763.5 49 109440.0 50 91369.0 51 82113.5 52 76388.5 53 72222.5 54 67913.5 55 62016.0 56 54953.5 57 47619.5 58 41746.0 59 36282.0 60 30852.5 61 25161.0 62 19616.5 63 14926.0 64 11185.5 65 8580.5 66 6254.0 67 4686.5 68 3453.5 69 2224.0 70 1515.5 71 1053.0 72 762.0 73 578.5 74 418.0 75 307.5 76 217.0 77 122.5 78 71.5 79 47.5 80 37.0 81 32.5 82 24.5 83 19.5 84 16.0 85 10.5 86 7.5 87 6.0 88 5.0 89 6.0 90 5.5 91 5.0 92 3.5 93 0.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2860431647317983 2 0.08612831286098992 3 0.08987858081337345 4 0.10394208563481169 5 0.10611553637994305 6 0.08144047792051051 7 0.09162586474573396 8 0.08821653024356713 9 0.09324529863426322 10-11 0.08657578801439933 12-13 0.09109315622977039 14-15 0.09959518414454896 16-17 0.08832307194675983 18-19 0.09925425069433227 20-21 0.10159816816457198 22-23 0.11523550617323935 24-25 0.12861714409424424 26-27 0.1315790034430017 28-29 0.1225655753528981 30-31 0.11495849774493831 32-33 0.10946094586019428 34-35 0.3833370480873846 36-37 0.586703851141637 38-39 0.570530820596983 40-41 0.5344344915552915 42-43 0.560792908925169 44-45 0.5812702242788086 46-47 0.5705095122563445 48-49 0.5922653280482967 50-51 0.5836567584303254 52-53 0.5866186177790828 54-55 0.5766676227008832 56-57 0.5507140638031381 58-59 0.5452591285996713 60-61 0.4926701439037477 62-63 0.566567469238214 64-65 0.6005116558754127 66-67 0.6132540435772613 68-69 0.6152144109160073 70-71 0.6116346096887321 72-73 0.6037292153118327 74-75 0.6002985724690273 76-77 0.5841681586056504 78-79 0.5617730925945419 80-81 0.5565738574787374 82-83 0.569273628499309 84-85 0.5790541568524001 86-87 0.5699341870591038 88-89 0.5836354500896869 90-91 0.5972514797577156 92-93 0.6048585573656754 94-95 0.6045602405967359 96-97 0.6013426811603159 98-99 0.6052207991565307 100-101 0.5824847996952055 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2346499.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.53432608012285 #Duplication Level Percentage of deduplicated Percentage of total 1 72.13181967284117 37.8939653544566 2 14.778848464513908 15.527936886469934 3 5.556645045586246 8.75743808208981 4 2.5977247638955063 5.458788792515867 5 1.466592034645195 3.852321208728076 6 0.8796881780653798 2.7728295357189503 7 0.5871506381695479 2.1591894158625884 8 0.3895986724465956 1.637384295895394 9 0.28579183973749617 1.3512493529827052 >10 1.2173434052314316 11.058406298928608 >50 0.0687115946714663 2.451557719620645 >100 0.03592737628925597 3.334456411141909 >500 0.0022014603036171953 0.8237865753960528 >1k 0.0016307113360123804 1.5749692486861762 >5k 2.4460670040185703E-4 0.8652072086326 >10k+ 8.153556680061901E-5 0.48051361287392974 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11218 0.4780739305663459 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8741 0.3725124110430049 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5976 0.25467728731186334 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5482 0.23362464676098307 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3545 0.15107613512726834 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3187 0.13581936323007168 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2866 0.12213940854012723 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2576 0.10978057096977241 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.261668127708557E-5 2 0.0 0.0 0.0 0.0 4.261668127708557E-5 3 0.0 0.0 0.0 0.0 4.261668127708557E-5 4 0.0 0.0 0.0 0.0 4.261668127708557E-5 5 0.0 0.0 0.0 4.261668127708557E-5 4.261668127708557E-5 6 0.0 0.0 0.0 4.261668127708557E-5 8.523336255417114E-5 7 0.0 0.0 0.0 4.261668127708557E-5 8.523336255417114E-5 8 0.0 0.0 0.0 4.261668127708557E-5 8.523336255417114E-5 9 0.0 0.0 0.0 4.261668127708557E-5 8.523336255417114E-5 10-11 0.0 0.0 0.0 4.261668127708557E-5 8.523336255417114E-5 12-13 0.0 0.0 0.0 4.261668127708557E-5 1.7046672510834227E-4 14-15 0.0 0.0 0.0 4.261668127708557E-5 1.9177506574688503E-4 16-17 0.0 0.0 0.0 6.392502191562835E-5 2.557000876625134E-4 18-19 0.0 0.0 0.0 1.7046672510834227E-4 2.98316768939599E-4 20-21 0.0 0.0 0.0 3.1962510957814173E-4 3.4093345021668454E-4 22-23 0.0 0.0 0.0 5.966335378791978E-4 3.4093345021668454E-4 24-25 0.0 0.0 0.0 0.0013211171195896525 3.4093345021668454E-4 26-27 0.0 0.0 0.0 0.0025996175579022195 3.4093345021668454E-4 28-29 0.0 0.0 0.0 0.008502027914778571 3.4093345021668454E-4 30-31 0.0 0.0 0.0 0.02437674169049294 3.4093345021668454E-4 32-33 0.0 0.0 0.0 0.05184319277357459 4.261668127708556E-4 34-35 0.0 0.0 0.0 0.09465164911640704 4.261668127708556E-4 36-37 0.0 0.0 0.0 0.16239086400633454 4.261668127708556E-4 38-39 0.0 0.0 0.0 0.2601322225153303 4.261668127708556E-4 40-41 0.0 0.0 0.0 0.36501187513823785 4.261668127708556E-4 42-43 0.0 0.0 0.0 0.47327955392267373 4.474751534093984E-4 44-45 0.0 0.0 0.0 0.6105905009974435 4.687834940479412E-4 46-47 0.0 0.0 0.0 0.7724486564878144 4.687834940479412E-4 48-49 0.0 0.0 0.0 0.9464951828234318 4.687834940479412E-4 50-51 0.0 0.0 0.0 1.1294699038865987 5.114001753250268E-4 52-53 0.0 0.0 0.0 1.312295466565296 5.114001753250268E-4 54-55 0.0 0.0 0.0 1.511336676469924 5.540168566021123E-4 56-57 0.0 0.0 0.0 1.7545500765182513 5.540168566021123E-4 58-59 0.0 0.0 0.0 2.0256347861217927 5.540168566021123E-4 60-61 0.0 0.0 0.0 2.3281919148484613 5.540168566021123E-4 62-63 0.0 0.0 0.0 2.6285116678080835 5.540168566021123E-4 64-65 0.0 0.0 0.0 2.944322584411926 5.540168566021123E-4 66-67 0.0 0.0 0.0 3.300576731547723 5.540168566021123E-4 68-69 0.0 0.0 0.0 3.701684935727652 5.540168566021123E-4 70-71 0.0 0.0 0.0 4.151290923200905 5.540168566021123E-4 72-73 0.0 0.0 0.0 4.6012804608056515 5.966335378791978E-4 74-75 0.0 0.0 0.0 5.076499073726433 6.392502191562835E-4 76-77 0.0 0.0 0.0 5.5870895321071945 6.392502191562835E-4 78-79 0.0 0.0 0.0 6.166015838915763 6.392502191562835E-4 80-81 0.0 0.0 2.1308340638542784E-5 6.785939393112889 6.392502191562835E-4 82-83 0.0 0.0 4.261668127708557E-5 7.421481960998065 6.818669004333691E-4 84-85 0.0 0.0 4.261668127708557E-5 8.07432690148174 6.818669004333691E-4 86-87 0.0 0.0 4.261668127708557E-5 8.76931973974845 7.031752410719119E-4 88-89 0.0 0.0 4.261668127708557E-5 9.51334306982445 7.244835817104546E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9300 0.0 25.136328 1 TACATGG 9455 0.0 23.92904 2 ACATGGG 9315 0.0 23.678926 3 GAGTACT 8755 0.0 22.975687 12-13 GTACCGT 135 3.8926298E-4 21.033949 6 GTACTTT 9630 0.0 20.544254 14-15 CATGGGA 6280 0.0 20.500679 4 AGTACTT 9295 0.0 20.087847 12-13 TTTGCGT 405 0.0 19.867939 3 GTATAGG 615 0.0 19.27537 1 GTATCAA 21605 0.0 19.072313 1 CATGGGG 3375 0.0 18.932978 4 ATGGGAT 2800 0.0 17.580622 5 GGTATCA 17385 0.0 17.183187 1 ACTTTTT 11890 0.0 17.058174 16-17 TAGACCG 195 2.3388519E-4 16.991133 5 CGGGTCG 195 2.3411388E-4 16.988958 6 CAACGCA 24380 0.0 16.94882 5 ATCAACG 24325 0.0 16.948225 3 TCAACGC 24475 0.0 16.863695 4 >>END_MODULE