FastQCFastQC Report
Fri 27 May 2016
SRR939002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346499
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111350.47453674602034773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90700.38653329918316603No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62710.26724920828860355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57660.24572778424367536No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA33320.1419987820152491No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31490.13419992934154246No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA27840.11864484067540622No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24400.10398470231608876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG98950.024.5117551
TACATGG98300.024.1861552
ACATGGG98050.022.7958563
GAGTACT93600.022.48586812-13
TTTGCGT3950.021.6250953
AGTACTT98700.020.17003612-13
GTACTTT105300.020.0550414-15
CATGGGA68850.019.7815634
CATGGGG33500.019.6903134
CTTTGCG4350.019.636582
GTATCAA221800.019.0776061
ATAACGC1250.00606603818.9820273
CCATGCG2001.29643795E-518.9816239
GTACCGT1300.00760774718.251566
GGTATCA178100.017.9259951
TTGCGTT4950.017.256394
ACTTTTT125550.017.04678316-17
ATCAACG249700.016.8572863
CAACGCA251150.016.7788565
TCAACGC251050.016.766644
Produced by FastQC (version 0.11.5)