##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939002_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2346499 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14844839055972 33.0 31.0 34.0 30.0 34.0 2 32.367353661774416 34.0 31.0 34.0 30.0 34.0 3 32.46408713577121 34.0 31.0 34.0 30.0 34.0 4 36.04161774626795 37.0 35.0 37.0 35.0 37.0 5 35.95846152075922 37.0 35.0 37.0 35.0 37.0 6 36.004378437834404 37.0 35.0 37.0 35.0 37.0 7 35.942571891145064 37.0 35.0 37.0 35.0 37.0 8 35.91588575149616 37.0 35.0 37.0 35.0 37.0 9 37.59090628208237 39.0 37.0 39.0 35.0 39.0 10-11 37.51155572621169 39.0 37.0 39.0 34.5 39.0 12-13 37.44338565667405 39.0 37.0 39.0 34.0 39.0 14-15 38.69456624528713 40.0 38.0 41.0 34.0 41.0 16-17 38.689079773739515 40.0 38.0 41.0 34.0 41.0 18-19 38.79886950729576 40.0 38.0 41.0 34.0 41.0 20-21 38.801674537257426 40.0 38.0 41.0 34.0 41.0 22-23 38.73916907699513 40.0 38.0 41.0 34.0 41.0 24-25 38.679343140568136 40.0 38.0 41.0 34.0 41.0 26-27 38.517762419672884 40.0 38.0 41.0 34.0 41.0 28-29 38.33607898405241 40.0 38.0 41.0 34.0 41.0 30-31 38.15786902103943 40.0 37.5 41.0 33.5 41.0 32-33 37.98127103399575 40.0 37.0 41.0 33.0 41.0 34-35 37.94028167069324 40.0 37.0 41.0 33.0 41.0 36-37 37.82421279531762 40.0 37.0 41.0 33.0 41.0 38-39 37.68869409277396 40.0 37.0 41.0 33.0 41.0 40-41 37.530871737000524 40.0 37.0 41.0 33.0 41.0 42-43 37.3515224170136 40.0 37.0 41.0 32.0 41.0 44-45 37.08558345859086 40.0 36.5 41.0 31.0 41.0 46-47 36.751943640291344 40.0 36.0 41.0 30.5 41.0 48-49 36.443391836092836 40.0 36.0 41.0 30.0 41.0 50-51 36.173899924952025 39.0 35.5 41.0 29.5 41.0 52-53 35.94677410900239 39.0 35.0 41.0 29.0 41.0 54-55 35.70889993986786 39.0 35.0 40.0 28.0 41.0 56-57 35.4423997197527 38.5 35.0 40.0 28.0 41.0 58-59 35.15224404527767 38.0 34.5 40.0 27.0 41.0 60-61 34.76009812917031 38.0 34.0 40.0 26.0 41.0 62-63 34.46094735177812 37.5 34.0 40.0 26.0 41.0 64-65 34.21746823672203 37.0 34.0 40.0 26.0 41.0 66-67 33.78051769892082 36.5 33.0 39.0 25.5 41.0 68-69 33.369034676767384 36.0 33.0 39.0 24.0 40.0 70-71 32.91582885822666 35.5 32.5 38.5 23.0 40.0 72-73 32.40374979916889 35.0 32.0 38.0 22.0 40.0 74-75 31.93530276381963 35.0 31.5 37.0 20.5 39.0 76-77 29.92709521717248 33.0 29.0 35.5 18.0 37.5 78-79 30.90158146242551 34.0 31.0 36.0 18.0 38.0 80-81 30.895338970952043 34.0 31.0 36.0 18.0 37.5 82-83 30.564396149327145 34.0 31.0 35.0 17.0 37.0 84-85 30.1606810827535 34.0 30.5 35.0 10.5 36.5 86-87 29.59035056055852 34.0 30.0 35.0 6.0 36.0 88-89 29.018973372671372 34.0 29.0 35.0 2.0 36.0 90-91 28.557031134468843 34.0 29.0 35.0 2.0 35.0 92-93 28.202993054759453 33.5 29.0 35.0 2.0 35.0 94-95 27.82194430937324 33.0 27.5 35.0 2.0 35.0 96-97 27.210766124340985 33.0 27.0 35.0 2.0 35.0 98-99 26.35128951685042 33.0 25.5 34.5 2.0 35.0 100-101 22.129664022869818 28.5 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2145214044976953 1101 2 0.21856296424328292 1101 3 0.20193109430557854 1101 4 0.14209884841284293 1101 5 0.2364812483124794 1101 6 0.17761817316878137 1101 7 0.17121689907647664 1101 8 0.22842703769067896 1101 9 0.25287549617956273 1101 10-11 0.32188131199021797 1101 12-13 0.25891397374196856 1101 14-15 0.38576207493726855 1101 16-17 0.3285797595300153 1101 18-19 0.368718651082915 1101 20-21 0.3377353775182357 1101 22-23 0.3524122106162295 1101 24-25 0.3440460915223298 1101 26-27 0.38597498171434097 1101 28-29 0.38869616968575116 1101 30-31 0.3210116546745496 1101 32-33 0.38597013717765094 1101 34-35 0.5028864916963087 1101 36-37 0.4729605898755054 1101 38-39 0.35962308887758354 1101 40-41 0.36885160412261087 1101 42-43 0.3624221173159654 1101 44-45 0.5953633750763316 1101 46-47 0.5533809415826028 1101 48-49 0.3564391433651295 1101 50-51 0.4656214848608613 1101 52-53 0.546082268085712 1101 54-55 0.5618486425137235 1101 56-57 0.3411782413765323 1101 58-59 0.36421489660398265 1101 60-61 0.5681195170006674 1101 62-63 0.4174010255434979 1101 64-65 0.4431698061947813 1101 66-67 0.4090605765762163 1101 68-69 0.358570890175379 1101 70-71 0.24201485355972352 1101 72-73 0.1546217323473016 1101 74-75 0.1484032690895809 1101 76-77 -0.02859186625170196 1101 78-79 0.2484621464489294 1101 80-81 0.37873314530916957 1101 82-83 0.36826074312039125 1101 84-85 0.30139839312149874 1101 86-87 0.20217900659644528 1101 88-89 0.33790052003624993 1101 90-91 0.3265398761294378 1101 92-93 0.5105533193342744 1101 94-95 0.49500928689628054 1101 96-97 0.36726730858968537 1101 98-99 0.28587178842045446 1101 100-101 0.7233982820843856 1102 1 0.018671873549578777 1102 2 0.0793565891724839 1102 3 0.11302461758747029 1102 4 0.12003258885269474 1102 5 0.08503581325942378 1102 6 0.10519252701003978 1102 7 0.06530971625875281 1102 8 0.12820098235626887 1102 9 0.17318193823930983 1102 10-11 0.17086845994266042 1102 12-13 0.1294222878709519 1102 14-15 0.11975973679055585 1102 16-17 0.11469365107001295 1102 18-19 0.08288991515641442 1102 20-21 0.17708348980100652 1102 22-23 0.1864600495117159 1102 24-25 0.129221103355313 1102 26-27 0.15323950525228724 1102 28-29 0.19113635166804954 1102 30-31 0.18792092992666198 1102 32-33 0.1685160271864703 1102 34-35 0.1778291372679277 1102 36-37 0.1789974829337453 1102 38-39 0.23385717771164138 1102 40-41 0.26032693848534905 1102 42-43 0.1490358394437763 1102 44-45 0.11137153272535727 1102 46-47 0.2127215014445909 1102 48-49 0.022858157877671204 1102 50-51 0.0916744914744001 1102 52-53 0.042681092287608635 1102 54-55 0.0977271675156004 1102 56-57 0.2100247642885691 1102 58-59 0.007890649453599963 1102 60-61 -0.03229470224196973 1102 62-63 -0.0165107663200601 1102 64-65 0.08062257123431493 1102 66-67 0.14633009195393498 1102 68-69 0.20844777537082848 1102 70-71 0.20620745580838928 1102 72-73 0.143677188324709 1102 74-75 0.30528282608319657 1102 76-77 0.09503828756421129 1102 78-79 0.06081100104155368 1102 80-81 0.20150472855003443 1102 82-83 0.13642922935144952 1102 84-85 0.24587516813436494 1102 86-87 0.24348195436834885 1102 88-89 0.5098015057306213 1102 90-91 0.5772957648191692 1102 92-93 0.40867581856480584 1102 94-95 0.41134036226271675 1102 96-97 0.3705087713797859 1102 98-99 0.19694074047033894 1102 100-101 0.656948618192974 1103 1 -0.056867475964239134 1103 2 0.05214910628132685 1103 3 0.08185201574274714 1103 4 0.06548053562443101 1103 5 0.018290060886847925 1103 6 -0.012827680262844865 1103 7 0.02299136337482821 1103 8 0.03320604917493597 1103 9 0.07256558641542199 1103 10-11 0.12829030759808546 1103 12-13 0.06333926371355858 1103 14-15 0.10723998619803865 1103 16-17 0.0075335612673228525 1103 18-19 0.06695922890249051 1103 20-21 0.1589235700034095 1103 22-23 0.1508764820046835 1103 24-25 0.12245673375755928 1103 26-27 0.07361086639311765 1103 28-29 0.12503270189188953 1103 30-31 0.16968204942691045 1103 32-33 0.2732216683557027 1103 34-35 0.10185435802455345 1103 36-37 0.004387661305763402 1103 38-39 0.21412325236054386 1103 40-41 0.22732844853064904 1103 42-43 0.1579356897726214 1103 44-45 0.2571573646336418 1103 46-47 0.39889354493922724 1103 48-49 0.38402836798398 1103 50-51 0.31678499478950073 1103 52-53 0.242510628840364 1103 54-55 0.36012920624342826 1103 56-57 0.22389101360937502 1103 58-59 0.19970911234081967 1103 60-61 0.26160265634545254 1103 62-63 0.17668732128922926 1103 64-65 0.30857065967696684 1103 66-67 0.3090212226878535 1103 68-69 0.2897281304481467 1103 70-71 0.23565292250572156 1103 72-73 0.31872681481350895 1103 74-75 0.34566716261329233 1103 76-77 0.174456711783602 1103 78-79 0.3744685357675941 1103 80-81 0.3984593871897957 1103 82-83 0.485854712115934 1103 84-85 0.4131710203432668 1103 86-87 0.22188797321557985 1103 88-89 0.4981738652347367 1103 90-91 0.6119670132899557 1103 92-93 0.6770444946250898 1103 94-95 0.5094724728926998 1103 96-97 0.22501690662384277 1103 98-99 0.41477852560389294 1103 100-101 0.6953293529483524 1104 1 -0.017771422745560983 1104 2 0.03554341934667349 1104 3 0.06830883641365659 1104 4 0.09419570213560746 1104 5 0.052032607432906275 1104 6 0.0455481766308381 1104 7 0.04054683329735553 1104 8 0.14027273477100977 1104 9 0.1970112928203207 1104 10-11 0.08650137724477958 1104 12-13 0.054442881934463117 1104 14-15 0.12117652536162637 1104 16-17 0.12120116764382516 1104 18-19 0.15234751836017324 1104 20-21 0.19673022639411641 1104 22-23 0.17681206793744053 1104 24-25 0.16200851244575176 1104 26-27 0.15555890517136817 1104 28-29 0.06209675089201028 1104 30-31 0.06665239786320853 1104 32-33 0.014013864031682033 1104 34-35 0.10669433693178831 1104 36-37 0.059054233325390726 1104 38-39 0.16104735896014688 1104 40-41 0.16423835807513143 1104 42-43 0.1933648464675528 1104 44-45 0.2791164568333784 1104 46-47 0.18903500845279808 1104 48-49 0.17542748776656225 1104 50-51 0.13183042536589795 1104 52-53 0.15092390511505727 1104 54-55 0.15918327674194188 1104 56-57 0.17199266452407613 1104 58-59 0.14141975607586232 1104 60-61 0.1046969045099786 1104 62-63 0.21007342034411636 1104 64-65 0.151371738653161 1104 66-67 0.27343021956450286 1104 68-69 0.2500065955436739 1104 70-71 0.03280357354210395 1104 72-73 0.03873056689236165 1104 74-75 -0.056568772161380565 1104 76-77 -0.10142928586374111 1104 78-79 0.18683204122332242 1104 80-81 0.3691868848574238 1104 82-83 0.37038235581041334 1104 84-85 0.3057082369092008 1104 86-87 0.1489086197900349 1104 88-89 0.35334088344752956 1104 90-91 0.4808917891792994 1104 92-93 0.3451350921182943 1104 94-95 0.2930274154960344 1104 96-97 0.426578988317754 1104 98-99 0.4840956472523743 1104 100-101 1.0769715465961838 1105 1 -0.019539383996807658 1105 2 0.01606704050065133 1105 3 0.03559888978197989 1105 4 -0.023034866764767514 1105 5 0.044862312180818265 1105 6 0.005704631632760027 1105 7 -0.019844600150079827 1105 8 0.05626961750867565 1105 9 -0.07452810207565363 1105 10-11 -0.0693383599581665 1105 12-13 -0.12258190819289183 1105 14-15 -0.032622194193272946 1105 16-17 0.029967777423742348 1105 18-19 -0.018285404270564243 1105 20-21 0.01595840802396964 1105 22-23 -0.1026932146384496 1105 24-25 -0.01281456318731955 1105 26-27 0.04264959185690742 1105 28-29 0.06479397199074555 1105 30-31 0.036088232569390755 1105 32-33 0.014285144772728131 1105 34-35 0.03138196948942351 1105 36-37 0.08858693991800948 1105 38-39 0.2201802470642349 1105 40-41 0.23246072936475315 1105 42-43 0.17741926045182055 1105 44-45 0.022046486131699794 1105 46-47 0.11582432689692723 1105 48-49 -0.009762063485773353 1105 50-51 0.023936353096708274 1105 52-53 0.20225170103302048 1105 54-55 0.13927314465288987 1105 56-57 0.10993250925283604 1105 58-59 0.044333212587091 1105 60-61 -0.03481469150860761 1105 62-63 -0.0657713478834836 1105 64-65 -0.07346968542275789 1105 66-67 -0.030178800766755387 1105 68-69 -0.08338599208000375 1105 70-71 0.0011603027841928792 1105 72-73 -0.1293261751739081 1105 74-75 -0.11811428320497441 1105 76-77 -0.3059097585827679 1105 78-79 -0.16728126534364662 1105 80-81 -0.23743670571581887 1105 82-83 -0.1587366229902294 1105 84-85 -0.20957266196720425 1105 86-87 -0.21502070911073545 1105 88-89 -0.060733062750099265 1105 90-91 0.19438203786975095 1105 92-93 0.08374130099459975 1105 94-95 0.10836059756980276 1105 96-97 -0.16267750515671153 1105 98-99 0.008052202874790026 1105 100-101 0.49866759378916825 1106 1 -0.006616100437994987 1106 2 -0.04268762525455827 1106 3 -0.04886325790282342 1106 4 0.019174675061073287 1106 5 -0.0654912124939031 1106 6 -0.050208971829739824 1106 7 0.057599468012135446 1106 8 0.0474201526859801 1106 9 -0.06835306889501425 1106 10-11 0.024343855410883464 1106 12-13 0.06310166737885936 1106 14-15 0.04267051514352005 1106 16-17 -0.01076614233051032 1106 18-19 -0.048964551230263 1106 20-21 -0.02816185779362712 1106 22-23 0.16355453468387537 1106 24-25 -6.773922149605482E-4 1106 26-27 0.013475164288472286 1106 28-29 0.04917336689840823 1106 30-31 0.053497740875123156 1106 32-33 0.07026315030276464 1106 34-35 0.14590310326065747 1106 36-37 0.0016998216118011555 1106 38-39 0.11364642453384022 1106 40-41 0.13850111954816668 1106 42-43 0.2502911697830257 1106 44-45 0.20191467139395058 1106 46-47 0.22540425083265347 1106 48-49 0.028750857857964718 1106 50-51 0.08535061086094231 1106 52-53 0.15152120312356487 1106 54-55 0.20004570952730205 1106 56-57 0.07859372605619086 1106 58-59 0.025033930359775525 1106 60-61 0.20362067577909926 1106 62-63 0.19570589587873144 1106 64-65 0.0630962566689206 1106 66-67 0.22783945062399624 1106 68-69 0.2228611636569937 1106 70-71 0.23427958976795793 1106 72-73 0.19300345710967548 1106 74-75 0.20999068207804683 1106 76-77 0.07788980433669579 1106 78-79 0.38568342176123593 1106 80-81 0.34041588881458296 1106 82-83 0.43798209166864766 1106 84-85 0.3366049783332805 1106 86-87 0.2903165152455074 1106 88-89 0.36917062124214794 1106 90-91 0.6554102517947982 1106 92-93 0.6132439463289217 1106 94-95 0.6280102115259645 1106 96-97 0.6257560774953888 1106 98-99 0.6027707677794645 1106 100-101 1.2538422611195124 1107 1 -0.16792831593807023 1107 2 -0.15440604082940723 1107 3 -0.11914048947787137 1107 4 -0.08248624855510656 1107 5 -0.20781650555655773 1107 6 -0.04762733572577815 1107 7 -0.18579813640316445 1107 8 -0.13363025806551576 1107 9 -0.10070235390875126 1107 10-11 -0.09117134931767623 1107 12-13 -0.03565287516186544 1107 14-15 -0.012164718552838849 1107 16-17 -0.0625380956955155 1107 18-19 -0.16757357115147897 1107 20-21 -0.10677328209511217 1107 22-23 -0.0737327367151579 1107 24-25 -0.13169700884689917 1107 26-27 -0.1667231509937679 1107 28-29 -0.13303199137441624 1107 30-31 -0.17673655369180352 1107 32-33 -0.17304208135600874 1107 34-35 -0.17689589496543334 1107 36-37 -0.14372111105345908 1107 38-39 -0.14492249293619608 1107 40-41 -0.19103619280407003 1107 42-43 -0.13077746586368022 1107 44-45 -0.1350144779336233 1107 46-47 -0.3406880723767358 1107 48-49 -0.10677956437002933 1107 50-51 -0.11778610677205137 1107 52-53 -0.08866611192686946 1107 54-55 -0.1607022639853426 1107 56-57 -0.1310162861519899 1107 58-59 -0.17681677030006426 1107 60-61 -0.19094456060778242 1107 62-63 -0.2375699589389555 1107 64-65 -0.21196369440233553 1107 66-67 -0.04093041503989525 1107 68-69 -0.2740526068548874 1107 70-71 -0.0878481216172986 1107 72-73 -0.14282566903828098 1107 74-75 -0.13134144061525177 1107 76-77 -0.31998721622133175 1107 78-79 -0.19343682087376735 1107 80-81 -0.06354563495565202 1107 82-83 -0.012079006860435726 1107 84-85 -0.01900334427924477 1107 86-87 -0.05686620209981186 1107 88-89 -0.05663504299382893 1107 90-91 0.0549337543239119 1107 92-93 0.09500935294284929 1107 94-95 0.04210700473178619 1107 96-97 -0.038229570735712315 1107 98-99 -0.048662459872520714 1107 100-101 0.3890171154499775 1108 1 0.009387056771963387 1108 2 -0.069329832073457 1108 3 -0.03286580886548762 1108 4 -0.08221329663820143 1108 5 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-4.341594288402973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 14.0 5 53.0 6 130.0 7 351.0 8 768.0 9 1404.0 10 2441.0 11 3613.0 12 5671.0 13 7819.0 14 9149.0 15 11835.0 16 15827.0 17 22263.0 18 21569.0 19 14229.0 20 12449.0 21 13249.0 22 14149.0 23 15559.0 24 17906.0 25 21459.0 26 25572.0 27 30700.0 28 38230.0 29 46313.0 30 56236.0 31 68026.0 32 84466.0 33 106885.0 34 143341.0 35 204592.0 36 320312.0 37 527844.0 38 445882.0 39 36180.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.39657345066099 20.11754159633212 14.703995858017283 22.781889094989612 2 15.301221095768632 21.72947868292294 39.67659905246071 23.292701168847717 3 18.98675430929227 25.537108688305427 31.076936320876335 24.39920068152597 4 12.634993665030327 17.595873682452027 37.85132659336313 31.917806059154515 5 12.897322678540302 38.12043280018172 34.827084062259885 14.155160459018099 6 30.393492603235718 37.23172266427559 17.770048058831474 14.604736673657223 7 26.86167775907852 33.96881907897681 21.63840683503381 17.53109632691086 8 25.07488816317416 35.4118625237002 20.543669526388037 18.969579786737604 9 25.908811382404167 17.088649941892157 21.423917078166237 35.578621597537435 10-11 24.234785525158973 27.004912424850808 27.948722756753785 20.811579293236434 12-13 25.554134052475625 24.84471546759662 28.23468068812303 21.366469791804725 14-15 22.362738379555996 25.977469115283053 27.30414393036683 24.355648574794117 16-17 21.10733707233085 29.187151599870443 28.054670053357427 21.650841274441284 18-19 20.88505045175813 27.952707416453194 30.422003163010086 20.740238968778595 20-21 21.97533431721045 26.991914337061303 30.169137084652498 20.863614261075757 22-23 21.459800323801545 27.04188239585868 30.041350965843154 21.45696631449662 24-25 21.51722630182242 27.072332014631158 30.29824432058143 21.112197362964995 26-27 21.481321747846472 27.03154785064899 30.451856148244683 21.035274253259857 28-29 21.02939315124362 27.42626355263735 30.423025963360733 21.12131733275829 30-31 21.309022505443213 27.305914044710867 30.177617804226635 21.207445645619284 32-33 21.045843190216573 27.36753776583753 30.168881384564834 21.41773765938106 34-35 21.62199366316498 27.16760862574978 30.041580019429436 21.168817691655807 36-37 21.58525712113321 26.992412819020036 30.207076936452992 21.215253123393765 38-39 21.34423437408096 27.225856470766747 30.33288324344322 21.09702591170907 40-41 21.502371788028356 27.243545691519888 30.104287927672452 21.149794592779305 42-43 21.14915131370208 27.485452142241172 29.733543378495312 21.63185316556143 44-45 21.46681244471795 27.42820711535253 29.532840441007725 21.572139998921795 46-47 21.573248037120983 27.636347829385134 29.044643072129666 21.74576106136422 48-49 21.685611993067088 27.65303822008115 28.9967905240553 21.66455926279646 50-51 21.43716788061111 27.95894962801776 29.015978381262507 21.587904110108617 52-53 21.6277313563739 28.173248742062114 28.569924811389225 21.629095090174765 54-55 21.24545972531844 27.748978371608086 28.83169777613372 22.17386412693975 56-57 21.533058398916854 28.125645909075608 28.435362640256823 21.905933051750715 58-59 21.744568705481456 27.753661419973803 28.57953184623528 21.92223802830947 60-61 21.614191173070576 27.695668915246024 28.930537244754778 21.75960266692862 62-63 21.523490953970146 27.71281811754448 28.866110746264965 21.897580182220405 64-65 21.31881038664714 27.921151283819878 28.729122837970785 22.030915491562197 66-67 21.25553361936796 27.964746782643164 28.440379679537514 22.339339918451365 68-69 21.19472616978641 27.897414566831486 28.605291218153006 22.302568045229098 70-71 21.33890134598424 27.80082748827668 28.407348225451667 22.45292294028741 72-73 21.373011452380762 27.530312179975358 28.494024502034733 22.602651865609147 74-75 21.208191437541632 28.10084300057234 28.11034652049713 22.580619041388893 76-77 21.300907610456946 27.63672881326642 28.332824494580173 22.729539081696462 78-79 21.193830887879013 27.727550413989537 28.401661796640226 22.676956901491224 80-81 21.176655093396587 27.869967129753732 28.25456136993879 22.69881640691089 82-83 21.18858776415417 27.803335948577008 28.223792126056736 22.78428416121209 84-85 21.16205888005919 27.56792140120239 28.0979450662455 23.172074652492928 86-87 21.08784962647328 27.537758122669725 28.26067658185493 23.11371566900206 88-89 21.153795942895957 27.393691812299547 28.11098075287517 23.34153149192932 90-91 21.043375454177145 27.375911694814338 28.21011178182821 23.370601069180307 92-93 21.10113833417359 27.55914236485931 28.09826469135508 23.24145460961202 94-95 21.01543618812537 27.313968597472233 28.032677618869645 23.63791759553275 96-97 20.823187849924622 27.209264911916215 28.140124334776978 23.827422903382185 98-99 20.828689890769184 27.208130069520593 27.83544761791929 24.12773242179093 100-101 20.91381768521709 26.726504812600403 28.454677004632224 23.905000497550283 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1788.0 1 1207.0 2 726.5 3 791.5 4 665.0 5 718.5 6 1235.5 7 2289.5 8 3461.5 9 3971.5 10 3486.5 11 2946.5 12 3052.0 13 3298.0 14 3546.0 15 3740.5 16 3892.5 17 4179.0 18 4534.5 19 4892.5 20 5438.0 21 6171.0 22 7222.0 23 8889.5 24 10920.0 25 13301.0 26 16530.5 27 20164.0 28 23681.0 29 27325.5 30 31052.0 31 35548.5 32 39999.5 33 44420.5 34 49775.0 35 54675.0 36 60068.5 37 64942.0 38 67309.5 39 68255.0 40 70508.0 41 73600.5 42 75979.5 43 79155.0 44 82695.5 45 85008.5 46 86132.5 47 91979.0 48 109066.0 49 108955.0 50 92800.0 51 85157.5 52 78414.5 53 73639.0 54 69074.5 55 62375.5 56 55629.5 57 48362.5 58 41904.0 59 36569.0 60 31059.0 61 25132.0 62 19628.5 63 14907.5 64 11035.0 65 8355.5 66 5952.5 67 4442.0 68 3227.5 69 2063.5 70 1386.5 71 950.0 72 683.5 73 493.0 74 348.0 75 247.5 76 161.0 77 82.5 78 45.5 79 27.5 80 13.5 81 10.0 82 7.0 83 4.5 84 3.0 85 2.0 86 4.0 87 3.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4028981047935669 2 0.0 3 0.0 4 0.0 5 5.114001753250268E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013211171195896525 16-17 0.0025143841953480482 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002535692535986591 36-37 0.007372685860935803 38-39 0.012827621064402757 40-41 0.0026635425798178475 42-43 8.310252849031685E-4 44-45 4.048584721323128E-4 46-47 4.048584721323128E-4 48-49 4.261668127708556E-4 50-51 6.818669004333691E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.130834063854278E-4 60-61 0.0023226091296011634 62-63 0.0 64-65 2.343917470239706E-4 66-67 0.001981675679384479 68-69 0.0019177506574688504 70-71 2.1308340638542784E-5 72-73 0.0 74-75 0.0 76-77 0.12476033443866799 78-79 1.065417031927139E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14604736673657223 88-89 0.43477538238882696 90-91 0.3320478721704122 92-93 0.0 94-95 0.0 96-97 4.90091834686484E-4 98-99 0.0 100-101 1.065417031927139E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2346499.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.05017081825459 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6572054350619 36.07062407000417 2 15.580120167420338 15.907355918314837 3 5.748929240092133 8.804514591862858 4 2.7989405688900635 5.7154557660792165 5 1.5345478102062404 3.916946391990356 6 0.9396889843657602 2.8782769900742537 7 0.5846033307925405 2.089086992851585 8 0.4256146881092047 1.7382162024586443 9 0.2989615078545904 1.3735832439653823 >10 1.3127502853351498 11.495049219193643 >50 0.07650264826188227 2.6844301948782574 >100 0.037440312320377644 3.4181516949050583 >500 0.002515189976739242 0.8639239071596801 >1k 0.0018444726496080165 1.6644177806101064 >5k 2.515189976738204E-4 0.9033857769835815 >10k+ 8.383966589127349E-5 0.47658125866831763 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11135 0.47453674602034773 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9070 0.38653329918316603 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6271 0.26724920828860355 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5766 0.24572778424367536 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3332 0.1419987820152491 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3149 0.13419992934154246 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2784 0.11864484067540622 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2440 0.10398470231608876 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.523336255417114E-5 2 0.0 0.0 0.0 0.0 8.523336255417114E-5 3 0.0 0.0 0.0 0.0 8.523336255417114E-5 4 0.0 0.0 0.0 0.0 8.523336255417114E-5 5 0.0 0.0 0.0 0.0 8.523336255417114E-5 6 0.0 0.0 0.0 0.0 8.523336255417114E-5 7 0.0 0.0 0.0 0.0 8.523336255417114E-5 8 0.0 0.0 0.0 4.261668127708557E-5 8.523336255417114E-5 9 0.0 0.0 0.0 4.261668127708557E-5 8.523336255417114E-5 10-11 0.0 0.0 0.0 4.261668127708557E-5 8.523336255417114E-5 12-13 0.0 0.0 0.0 4.261668127708557E-5 1.0654170319271392E-4 14-15 0.0 0.0 0.0 6.392502191562835E-5 1.491583844697995E-4 16-17 0.0 0.0 0.0 1.0654170319271392E-4 1.9177506574688503E-4 18-19 0.0 0.0 0.0 2.130834063854278E-4 2.130834063854278E-4 20-21 0.0 0.0 0.0 3.1962510957814173E-4 3.8355013149377005E-4 22-23 0.0 0.0 0.0 5.327085159635695E-4 4.261668127708556E-4 24-25 0.0 0.0 0.0 0.0012358837570354813 4.261668127708556E-4 26-27 0.0 0.0 0.0 0.0024717675140709626 4.474751534093984E-4 28-29 0.0 0.0 0.0 0.0084167945522244 4.687834940479412E-4 30-31 0.0 0.0 0.0 0.024270199987300227 4.687834940479412E-4 32-33 0.0 0.0 0.0 0.052333284608261074 5.114001753250268E-4 34-35 0.0 0.0 0.0 0.09610061627982794 5.114001753250268E-4 36-37 0.0 0.0 0.0 0.16492655654232113 5.114001753250268E-4 38-39 0.0 0.0 0.0 0.26379725710515967 5.540168566021123E-4 40-41 0.0 0.0 0.0 0.370552043704259 5.540168566021123E-4 42-43 0.0 0.0 0.0 0.47977859781742926 5.753251972406551E-4 44-45 0.0 0.0 0.0 0.6185385120556199 5.96633537879198E-4 46-47 0.0 0.0 0.0 0.7819521764126045 5.96633537879198E-4 48-49 0.0 2.1308340638542784E-5 0.0 0.9583213118778231 5.96633537879198E-4 50-51 0.0 4.261668127708557E-5 0.0 1.143022008532712 6.392502191562835E-4 52-53 0.0 4.261668127708557E-5 0.0 1.3277013968469622 7.244835817104546E-4 54-55 0.0 4.261668127708557E-5 0.0 1.5291291409031071 7.244835817104546E-4 56-57 0.0 4.261668127708557E-5 0.0 1.7740045915212408 7.244835817104546E-4 58-59 0.0 4.261668127708557E-5 0.0 2.0463251848818174 7.244835817104546E-4 60-61 0.0 4.261668127708557E-5 0.0 2.35073613924404 7.244835817104546E-4 62-63 0.0 4.261668127708557E-5 0.0 2.654017751552419 7.671002629875401E-4 64-65 0.0 4.261668127708557E-5 0.0 2.971959502220116 7.671002629875401E-4 66-67 0.0 4.261668127708557E-5 0.0 3.329726541541249 7.671002629875401E-4 68-69 0.0 4.261668127708557E-5 0.0 3.733050813147587 7.671002629875401E-4 70-71 0.0 4.261668127708557E-5 0.0 4.186321835210669 7.671002629875401E-4 72-73 0.0 4.261668127708557E-5 0.0 4.640807432690148 7.884086036260829E-4 74-75 0.0 4.261668127708557E-5 0.0 5.120799113913963 8.097169442646257E-4 76-77 0.0 4.261668127708557E-5 0.0 5.635821707147542 8.097169442646257E-4 78-79 0.0 4.261668127708557E-5 0.0 6.216878848019965 8.097169442646257E-4 80-81 0.0 4.261668127708557E-5 0.0 6.840893603619691 8.523336255417114E-4 82-83 0.0 4.261668127708557E-5 0.0 7.479078405744047 9.375669880958824E-4 84-85 0.0 4.261668127708557E-5 0.0 8.136632489508838 9.375669880958824E-4 86-87 0.0 4.261668127708557E-5 0.0 8.834139711970899 9.375669880958824E-4 88-89 0.0 4.261668127708557E-5 0.0 9.579526775847764 9.375669880958824E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9895 0.0 24.511755 1 TACATGG 9830 0.0 24.186155 2 ACATGGG 9805 0.0 22.795856 3 GAGTACT 9360 0.0 22.485868 12-13 TTTGCGT 395 0.0 21.625095 3 AGTACTT 9870 0.0 20.170036 12-13 GTACTTT 10530 0.0 20.05504 14-15 CATGGGA 6885 0.0 19.781563 4 CATGGGG 3350 0.0 19.690313 4 CTTTGCG 435 0.0 19.63658 2 GTATCAA 22180 0.0 19.077606 1 ATAACGC 125 0.006066038 18.982027 3 CCATGCG 200 1.29643795E-5 18.981623 9 GTACCGT 130 0.007607747 18.25156 6 GGTATCA 17810 0.0 17.925995 1 TTGCGTT 495 0.0 17.25639 4 ACTTTTT 12555 0.0 17.046783 16-17 ATCAACG 24970 0.0 16.857286 3 CAACGCA 25115 0.0 16.778856 5 TCAACGC 25105 0.0 16.76664 4 >>END_MODULE