FastQCFastQC Report
Fri 27 May 2016
SRR939001_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939001_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801846
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171230.6111328031590602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141590.5053454044226556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93610.3341011604492181No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA66830.23852131773123864No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA56370.20118878767783813No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT41890.1495085739901479No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28850.1029678290669794No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28770.10268230302450598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG131400.025.4300771
TACATGG135650.023.9550442
GAGTACT123150.023.8175912-13
GTATCAA257250.023.6539131
CATGGGG52050.023.2066334
ACATGGG132700.023.166823
GGTATCA192600.022.9437161
AGTACTT130450.021.97662712-13
GTACTTT138750.021.1402914-15
TATCAAC300250.020.1465442
ATCAACG300400.020.0891783
TCAACGC301700.019.9869144
CAACGCA301650.019.9431155
AACGCAG305100.019.7236146
CGCCGTT650.00751022618.29297494-95
ACGCAGA326400.018.1754067
CATGGGA72250.018.1607844
CGCAGAG324650.018.040048
ACTTTTT165800.017.86486216-17
ATGGGGG27950.017.7950885
Produced by FastQC (version 0.11.5)