##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939001_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2801846 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.874628726917898 33.0 31.0 34.0 30.0 34.0 2 31.960300815962047 33.0 31.0 34.0 30.0 34.0 3 31.990711124023232 34.0 31.0 34.0 30.0 34.0 4 35.53722652851013 37.0 35.0 37.0 33.0 37.0 5 35.58393573379836 37.0 35.0 37.0 33.0 37.0 6 35.6743739663065 37.0 35.0 37.0 33.0 37.0 7 35.60428231958502 37.0 35.0 37.0 33.0 37.0 8 35.5942542880658 37.0 35.0 37.0 33.0 37.0 9 37.20970246044929 39.0 37.0 39.0 34.0 39.0 10-11 37.10607238941755 39.0 37.0 39.0 33.0 39.0 12-13 37.00653818946509 39.0 37.0 39.0 33.0 39.0 14-15 38.21271529555871 40.0 38.0 41.0 33.0 41.0 16-17 38.13930387323215 40.0 38.0 41.0 33.0 41.0 18-19 38.109752641651255 40.0 38.0 41.0 33.0 41.0 20-21 38.21551148778342 40.0 38.0 41.0 33.0 41.0 22-23 38.15076203331661 40.0 38.0 41.0 33.0 41.0 24-25 38.06824465013423 40.0 38.0 41.0 33.0 41.0 26-27 37.94710719290068 40.0 38.0 41.0 33.0 41.0 28-29 37.82297760119578 40.0 38.0 41.0 33.0 41.0 30-31 37.60574474828381 40.0 37.0 41.0 32.0 41.0 32-33 37.39286527525067 40.0 37.0 41.0 32.0 41.0 34-35 37.07110330118072 40.0 36.0 41.0 31.0 41.0 36-37 36.80021814189645 40.0 36.0 41.0 30.0 41.0 38-39 36.561768741037156 39.0 35.5 41.0 30.0 41.0 40-41 36.41324790870019 39.0 35.0 41.0 30.0 41.0 42-43 36.19169433295049 39.0 35.0 41.0 30.0 41.0 44-45 36.07033023942073 39.0 35.0 41.0 29.5 41.0 46-47 35.79970348120489 39.0 35.0 41.0 27.5 41.0 48-49 35.52873766081362 39.0 35.0 41.0 26.5 41.0 50-51 34.4110641698366 38.0 34.0 40.0 24.5 40.5 52-53 34.51333906288926 38.5 34.5 39.5 24.0 40.5 54-55 35.01819247024997 39.0 35.0 40.5 24.5 41.0 56-57 34.95371872686793 39.0 35.0 41.0 25.0 41.0 58-59 34.767468661732295 39.0 35.0 40.5 24.0 41.0 60-61 34.476386639379896 38.0 34.0 40.0 23.5 41.0 62-63 34.111960293320905 38.0 34.0 40.0 22.5 41.0 64-65 33.736429125655015 37.0 34.0 40.0 22.0 41.0 66-67 33.346913249336325 37.0 33.0 39.5 20.5 41.0 68-69 32.917689801652195 36.0 33.0 39.0 18.5 41.0 70-71 32.48905168235513 36.0 32.0 39.0 17.5 40.5 72-73 32.052948306223826 35.0 32.0 38.5 14.0 40.0 74-75 31.584549257882124 35.0 31.5 37.5 9.5 39.5 76-77 31.156478621594477 35.0 31.0 37.0 7.5 39.0 78-79 30.767335178307444 35.0 31.0 36.5 4.5 39.0 80-81 30.326488857703097 35.0 30.5 36.0 2.0 38.5 82-83 29.86733871169222 34.0 30.0 35.5 2.0 37.0 84-85 29.453754060715685 34.0 30.0 35.0 2.0 37.0 86-87 29.008544009913464 34.0 29.0 35.0 2.0 36.0 88-89 28.53491305375099 34.0 29.0 35.0 2.0 36.0 90-91 28.087763210397718 34.0 28.5 35.0 2.0 35.5 92-93 27.613222318428637 34.0 27.0 35.0 2.0 35.0 94-95 27.111181164132503 34.0 26.0 35.0 2.0 35.0 96-97 26.499428412553723 33.0 25.0 35.0 2.0 35.0 98-99 25.701462535771057 33.0 22.0 35.0 2.0 35.0 100-101 22.688326553279516 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09801997516662553 1101 2 0.23237842587482405 1101 3 0.298889398458023 1101 4 0.19559034976344236 1101 5 0.10590920159173578 1101 6 0.22645635463103986 1101 7 0.2154495424666507 1101 8 0.10983481865437028 1101 9 0.3041251916665786 1101 10-11 0.17926776394246957 1101 12-13 0.20608356190926003 1101 14-15 0.3393015363700229 1101 16-17 0.2875311303735373 1101 18-19 0.3811283753544146 1101 20-21 0.26293988356945874 1101 22-23 0.3971156486160865 1101 24-25 0.38287331753982556 1101 26-27 0.3569201074096 1101 28-29 0.34072934279168265 1101 30-31 0.30422055268468284 1101 32-33 0.35885359730466604 1101 34-35 0.3277934538033662 1101 36-37 0.252896150389212 1101 38-39 0.4075600787126916 1101 40-41 0.25045975230460016 1101 42-43 0.3635599509384022 1101 44-45 0.5283796321880345 1101 46-47 0.5100313982565083 1101 48-49 0.5136925779023329 1101 50-51 0.3815441363565313 1101 52-53 0.4238312894812353 1101 54-55 0.5603214489778239 1101 56-57 0.5140871422308422 1101 58-59 0.5595163165059347 1101 60-61 0.5602854101018053 1101 62-63 0.5571483814506806 1101 64-65 0.5213814067823677 1101 66-67 0.557752210528804 1101 68-69 0.4893258265027782 1101 70-71 0.29451056090378813 1101 72-73 0.23770264689495946 1101 74-75 0.12041611175083844 1101 76-77 0.04959636427245684 1101 78-79 -0.003964336252462175 1101 80-81 -0.0814770923348398 1101 82-83 -0.12791193385842448 1101 84-85 -0.04477966497890762 1101 86-87 -0.14486553973510752 1101 88-89 -0.2035358124016824 1101 90-91 -0.10906141588171536 1101 92-93 -0.23532134669715532 1101 94-95 -0.561595423886768 1101 96-97 -0.6155794313186753 1101 98-99 -0.7312225746161118 1101 100-101 -0.6791128227657417 1102 1 0.23717557772642195 1102 2 0.21349183000863903 1102 3 0.31025456668064777 1102 4 0.2500892970699411 1102 5 0.3325232294985341 1102 6 0.29854747695672046 1102 7 0.3377718529511853 1102 8 0.33681496085990403 1102 9 0.4005737910572691 1102 10-11 0.3313623666517813 1102 12-13 0.37163891594025955 1102 14-15 0.4506800930402264 1102 16-17 0.5899672763633816 1102 18-19 0.44710416340395653 1102 20-21 0.4317561058336139 1102 22-23 0.5076925834765404 1102 24-25 0.5081577837543705 1102 26-27 0.5368844334774252 1102 28-29 0.5495762573825758 1102 30-31 0.4481822096414092 1102 32-33 0.48263331843280355 1102 34-35 0.4807345617908396 1102 36-37 0.27922758094943134 1102 38-39 0.37897436446198896 1102 40-41 0.43075266212590435 1102 42-43 0.3736686884169842 1102 44-45 0.534210347063734 1102 46-47 0.553134233788775 1102 48-49 0.5085573270083401 1102 50-51 0.342596161964309 1102 52-53 0.3172825310223075 1102 54-55 0.38170010368700247 1102 56-57 0.3267611614121648 1102 58-59 0.3680374659639014 1102 60-61 0.46857653561374946 1102 62-63 0.3636961901570217 1102 64-65 0.31128541437058743 1102 66-67 0.20304489976241058 1102 68-69 0.2804438323409997 1102 70-71 0.18424581890560887 1102 72-73 0.13565525607973683 1102 74-75 0.1698582393436645 1102 76-77 0.0986443445470151 1102 78-79 0.13232929415530492 1102 80-81 0.05311295768954949 1102 82-83 0.052039371407982316 1102 84-85 0.01594368496386167 1102 86-87 0.039880637357594395 1102 88-89 -0.11663268593875742 1102 90-91 -0.1694753303083978 1102 92-93 -0.24543743709889299 1102 94-95 -0.23472597381189075 1102 96-97 -0.22286149067636885 1102 98-99 -0.2249015116775439 1102 100-101 -0.1961421927917968 1103 1 0.2658630026512263 1103 2 0.1820124356727426 1103 3 0.35534192511840956 1103 4 0.19361952785715175 1103 5 0.12607064420718217 1103 6 0.17959736441798668 1103 7 0.14166471765826572 1103 8 0.17810268230230974 1103 9 0.3505707067636763 1103 10-11 0.31226542350579223 1103 12-13 0.33170232780101827 1103 14-15 0.38649244887228207 1103 16-17 0.42334955829698373 1103 18-19 0.504012646791459 1103 20-21 0.3701733223300536 1103 22-23 0.4298576998425361 1103 24-25 0.32979202178344025 1103 26-27 0.39266461237860995 1103 28-29 0.4538488244598895 1103 30-31 0.43320955957685214 1103 32-33 0.37088229658962035 1103 34-35 0.19323524929963298 1103 36-37 0.3077913302592634 1103 38-39 0.4289639264831848 1103 40-41 0.40918980346281586 1103 42-43 0.20508872149641633 1103 44-45 0.2356094768371335 1103 46-47 0.3861097840009293 1103 48-49 0.37096972471386636 1103 50-51 0.3057760777737286 1103 52-53 0.320379663176233 1103 54-55 0.33638172710113423 1103 56-57 0.2540290364128879 1103 58-59 0.3733289286696291 1103 60-61 0.4100446198602441 1103 62-63 0.41423059340090873 1103 64-65 0.199096362063095 1103 66-67 0.3409062900890305 1103 68-69 0.35626306174164313 1103 70-71 0.2032939907095752 1103 72-73 0.174690486005467 1103 74-75 0.12824952346423046 1103 76-77 0.0684951467824142 1103 78-79 0.07181758098063185 1103 80-81 0.06328960718647281 1103 82-83 0.07189530906817865 1103 84-85 0.10394017646595088 1103 86-87 0.07784434114750738 1103 88-89 0.030740787373900957 1103 90-91 0.05159316015323512 1103 92-93 0.005518390043313559 1103 94-95 -0.02886624140702665 1103 96-97 -0.032600914967904515 1103 98-99 -0.16628185308909238 1103 100-101 0.0625451835135955 1104 1 0.16135853316035664 1104 2 0.20218792671881047 1104 3 0.24872663019180408 1104 4 0.2830005257943071 1104 5 0.2056897658550625 1104 6 0.1961766343513247 1104 7 0.18129714314183332 1104 8 0.19095852253669676 1104 9 0.26632678317852054 1104 10-11 0.33329236003603313 1104 12-13 0.2666315484055204 1104 14-15 0.3830518377927348 1104 16-17 0.32312855019509357 1104 18-19 0.3531008856027853 1104 20-21 0.2572303344978337 1104 22-23 0.37128733899466937 1104 24-25 0.3206892085453674 1104 26-27 0.3493393261219211 1104 28-29 0.3981690582486408 1104 30-31 0.3982710711317594 1104 32-33 0.391749063907028 1104 34-35 0.44367300528635667 1104 36-37 0.31322168692164354 1104 38-39 0.30833774931794267 1104 40-41 0.2833497932980933 1104 42-43 0.23716195238522886 1104 44-45 0.22100261265584464 1104 46-47 0.38639925453815493 1104 48-49 0.3665434339428444 1104 50-51 0.16807054098638474 1104 52-53 0.2877105998260632 1104 54-55 0.371164829735001 1104 56-57 0.38545680704878293 1104 58-59 0.38723816362433183 1104 60-61 0.3438506379773827 1104 62-63 0.3666462112071187 1104 64-65 0.32814894958440277 1104 66-67 0.3361251307120696 1104 68-69 0.29161000783868474 1104 70-71 0.35202683773040633 1104 72-73 0.42201118318624964 1104 74-75 0.32571753446605456 1104 76-77 0.26891092901805536 1104 78-79 0.1951175301569883 1104 80-81 0.2703501321648005 1104 82-83 0.44055864728457195 1104 84-85 0.4366213478014167 1104 86-87 0.3403828325822289 1104 88-89 0.2818047952665097 1104 90-91 0.1498576822615192 1104 92-93 0.02024954792545941 1104 94-95 0.06400320162565265 1104 96-97 0.0019513137982762885 1104 98-99 0.0708367453742369 1104 100-101 0.3006990894599646 1105 1 0.18116985391507612 1105 2 0.17938676561576372 1105 3 0.2149312746039982 1105 4 0.27257236904539184 1105 5 0.12946335076072302 1105 6 0.21275138036003227 1105 7 0.23684866708958907 1105 8 0.2752618607079569 1105 9 0.23142837969520968 1105 10-11 0.2884302410229722 1105 12-13 0.27395012605428803 1105 14-15 0.36583122821362934 1105 16-17 0.3464558361167178 1105 18-19 0.32459217732992585 1105 20-21 0.31831886860523184 1105 22-23 0.18408850009772237 1105 24-25 0.25311301115785767 1105 26-27 0.20630231596226878 1105 28-29 0.25045135142715225 1105 30-31 0.3112572830330578 1105 32-33 0.25731873008990647 1105 34-35 0.28200029120799286 1105 36-37 0.35552937922933836 1105 38-39 0.43888201637165025 1105 40-41 0.4080413753009893 1105 42-43 0.44658502930830224 1105 44-45 0.42339013079807586 1105 46-47 0.3822446826949104 1105 48-49 0.315621808398582 1105 50-51 0.22861117959305233 1105 52-53 0.28596531680719295 1105 54-55 0.384314321752413 1105 56-57 0.39524272867432586 1105 58-59 0.2751336643500224 1105 60-61 0.42682959298463174 1105 62-63 0.3801440341389082 1105 64-65 0.4132868305713373 1105 66-67 0.4360888462418231 1105 68-69 0.41798156081401316 1105 70-71 0.2257931657420258 1105 72-73 0.21816502934009918 1105 74-75 0.2808155025357202 1105 76-77 0.2927752250993336 1105 78-79 0.3421131760205576 1105 80-81 0.41717524101821013 1105 82-83 0.4200071233368199 1105 84-85 0.4632940618797896 1105 86-87 0.43361940732824067 1105 88-89 0.4253461595625936 1105 90-91 0.5918315574429407 1105 92-93 0.5202168919022405 1105 94-95 0.3529291813063473 1105 96-97 0.41794913673006207 1105 98-99 0.3451037990752539 1105 100-101 0.5671141837995854 1106 1 0.0026813620354033674 1106 2 0.11999241977065012 1106 3 0.09006820360556844 1106 4 0.13532837200452974 1106 5 0.05223428765791738 1106 6 0.08723619406606531 1106 7 -0.004571024967077619 1106 8 0.0800248190104682 1106 9 0.17408039819670051 1106 10-11 0.07619367638183405 1106 12-13 0.11519773213019135 1106 14-15 0.10331299290731266 1106 16-17 0.2169499697063273 1106 18-19 0.34452117198551235 1106 20-21 0.21220369752176538 1106 22-23 0.26135019662074654 1106 24-25 0.232576910436741 1106 26-27 0.1563110965647425 1106 28-29 0.06537097780215362 1106 30-31 0.110556425023006 1106 32-33 0.11748003044873911 1106 34-35 0.07370365894929876 1106 36-37 0.27836191862105863 1106 38-39 0.2869640424092452 1106 40-41 0.371688505703311 1106 42-43 0.24375981860841733 1106 44-45 0.20353227976411148 1106 46-47 0.16460562768131837 1106 48-49 0.34851809358148245 1106 50-51 0.19473464730878476 1106 52-53 0.1739134213724114 1106 54-55 0.3549548909013893 1106 56-57 0.30254231315132785 1106 58-59 0.4305549604222634 1106 60-61 0.4666821203462348 1106 62-63 0.3838813212784373 1106 64-65 0.30287511019550095 1106 66-67 0.29746698599858945 1106 68-69 0.35587248775524927 1106 70-71 0.29187674048431234 1106 72-73 0.2645996207655301 1106 74-75 0.25268155340790344 1106 76-77 0.10720341429020408 1106 78-79 0.31230669496799024 1106 80-81 0.2585008354373386 1106 82-83 0.2989130740963084 1106 84-85 0.31093477621982757 1106 86-87 0.48746544948895476 1106 88-89 0.45239926492291005 1106 90-91 0.5464371562869452 1106 92-93 0.5499039014502785 1106 94-95 0.46758946878465224 1106 96-97 0.6341048921729993 1106 98-99 0.6108418190839657 1106 100-101 0.8041032668143053 1107 1 0.0935332341389703 1107 2 0.13623454338594243 1107 3 0.16133501673096617 1107 4 0.18891640947568789 1107 5 0.18851687712726317 1107 6 0.12972334860118906 1107 7 0.08317923531570415 1107 8 0.185124608353739 1107 9 0.20614960081032763 1107 10-11 0.14066710562389773 1107 12-13 0.2365023906839383 1107 14-15 0.23419787732768071 1107 16-17 0.2455022502536508 1107 18-19 0.28161016990312504 1107 20-21 0.27352358456056436 1107 22-23 0.33904209963816356 1107 24-25 0.17346027944619635 1107 26-27 0.1950070011435301 1107 28-29 0.22971051697113865 1107 30-31 0.1780009760582857 1107 32-33 0.23505300624957925 1107 34-35 0.30249650599732547 1107 36-37 0.2066022317121181 1107 38-39 0.34187763277628136 1107 40-41 0.1450880903399394 1107 42-43 0.18590724750330168 1107 44-45 0.1478310343247884 1107 46-47 0.19021202114813462 1107 48-49 0.212880317429061 1107 50-51 0.13745291196806164 1107 52-53 0.21495688471188856 1107 54-55 0.2230570983942144 1107 56-57 0.09792000441683513 1107 58-59 0.10375334471000741 1107 60-61 -0.03445901992543554 1107 62-63 -0.10091589323427996 1107 64-65 -0.15256981696981597 1107 66-67 -0.17494981282934674 1107 68-69 -0.027272530427687514 1107 70-71 -0.12719068812901213 1107 72-73 0.16529079954668902 1107 74-75 0.1928398892768861 1107 76-77 0.17247904176793938 1107 78-79 0.11303427814260303 1107 80-81 0.18753577635026275 1107 82-83 0.15656890741226093 1107 84-85 0.29987287427421805 1107 86-87 0.43144285990539544 1107 88-89 0.3406968129986865 1107 90-91 0.3837481665506992 1107 92-93 0.4535133419422479 1107 94-95 0.5485190518500929 1107 96-97 0.5262295500600231 1107 98-99 0.5224345929778877 1107 100-101 0.6907280854730118 1108 1 0.23491727891026315 1108 2 0.1584184925262555 1108 3 0.17439938018453915 1108 4 0.20439208663241004 1108 5 0.16430706846178822 1108 6 0.16014629521558277 1108 7 0.2759925893654298 1108 8 0.2785443235886831 1108 9 0.2576558639957014 1108 10-11 0.3138546802243454 1108 12-13 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-1.520862320120866 2316 78-79 -1.5026895880978905 2316 80-81 -1.406380637065972 2316 82-83 -1.1474780920954544 2316 84-85 -1.310620328432794 2316 86-87 -1.469289957314441 2316 88-89 -1.5796877420469215 2316 90-91 -1.690297311997952 2316 92-93 -1.8084330008690301 2316 94-95 -1.9222884172032835 2316 96-97 -1.9044924290260603 2316 98-99 -1.8904376518703074 2316 100-101 -1.8803169840187977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7863.0 3 1988.0 4 1895.0 5 2139.0 6 2566.0 7 3114.0 8 3820.0 9 4904.0 10 6298.0 11 8496.0 12 12039.0 13 17932.0 14 16447.0 15 17668.0 16 22615.0 17 31210.0 18 26712.0 19 17343.0 20 16771.0 21 17733.0 22 18932.0 23 21219.0 24 23663.0 25 27142.0 26 31784.0 27 36869.0 28 43980.0 29 51903.0 30 62835.0 31 76411.0 32 95015.0 33 121098.0 34 164275.0 35 232774.0 36 354343.0 37 561551.0 38 547432.0 39 91052.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.02511487875471 20.214041687569175 14.682244272354584 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28.010328109812686 31.409839847867705 20.17710076121505 20-21 21.352923129562452 27.17473489165479 31.146868826773023 20.32547315200974 22-23 21.198130343693126 27.037019837860722 30.8468107071223 20.918039111323854 24-25 21.050991064725412 27.200224065098915 31.21087144425711 20.537913425918568 26-27 20.902584528551273 27.25016521692536 31.236626359690646 20.61062389483273 28-29 20.488427836064286 27.328685892283676 31.59822318433659 20.584663087315448 30-31 20.703744239260967 27.461570664470123 31.26047934778183 20.57420574848708 32-33 20.59051853687955 27.666050970235307 30.914699405549058 20.828731087336088 34-35 21.10095635855443 27.349029515740764 30.87553230990901 20.674481815795797 36-37 21.264575511078867 27.073204195702605 30.82381510499569 20.838405188222833 38-39 20.983613141617802 27.485390392802632 30.95719822590455 20.573798239675018 40-41 20.834487378820743 27.371144434865492 31.165592216704912 20.62877596960885 42-43 20.688224907024868 27.61898118368702 30.597090535966075 21.095703373322042 44-45 20.757328568167157 27.860727210317176 30.267407529491013 21.114536692024654 46-47 20.988230391023745 27.812240027502057 29.71633038401194 21.483199197462266 48-49 21.198777675520812 27.92647686331034 29.57878879245326 21.295956668715593 50-51 21.152438906278363 28.207747709097536 29.480092662009653 21.159720722614452 52-53 21.103801418694122 28.355785527885143 29.522612118983776 21.017800934436963 54-55 20.668325665425765 28.137622053158022 29.677044017149047 21.51700826426717 56-57 20.96722110090641 28.747433559423353 29.08840640333984 21.1969389363304 58-59 21.09425531823351 27.969863940992813 29.39423839511467 21.541642345659003 60-61 21.13446012128997 28.062543075286012 29.617374979974358 21.185621823449658 62-63 21.233014153549522 27.950852876572164 29.656462561246062 21.159670408632252 64-65 20.868161014150736 27.9253592749445 29.74958508118203 21.456894629722733 66-67 20.69547469128173 28.29353452752772 29.376575566845293 21.634415214345257 68-69 20.703414505147837 28.14022218207477 29.59715617278181 21.55920713999559 70-71 20.81681479974496 28.19648649938411 29.27726414691529 21.70943455395564 72-73 20.959230041497882 28.017117204706516 29.085615083173355 21.938037670622247 74-75 20.832062706424555 28.3476205612667 28.996250185661015 21.82406654664773 76-77 21.046485082045212 27.912354314360865 29.26481488156757 21.776345722026356 78-79 20.750770678413957 28.039910141756774 29.395027530696694 21.81429164913257 80-81 20.797298419206573 28.373896314482483 29.07986414633407 21.748941119976877 82-83 20.835516639211708 28.27362283162309 29.01583923408733 21.87502129507787 84-85 20.7719145341909 27.840570704584522 29.00124007736361 22.38627468386097 86-87 20.868209324992087 27.88452209841463 29.27200000717321 21.975268569420074 88-89 21.185705380156143 27.55078397093816 29.1854048047587 22.078105844146993 90-91 20.74355599512267 27.6514739787867 29.532686107825718 22.072283918264908 92-93 20.940961593931487 27.789975647312236 29.247890127331406 22.021172631424868 94-95 20.83200434556576 27.53426456358314 29.17646290608275 22.45726818476835 96-97 21.052087787825997 27.188758319437706 29.01463991435894 22.744513978377352 98-99 20.859457949749252 27.251255353853455 28.984041000781563 22.90524569561573 100-101 21.199820659971305 26.726721664275466 29.729842180774753 22.34361549497848 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4141.0 1 2623.5 2 1269.5 3 1452.5 4 1351.5 5 1434.5 6 2272.0 7 4286.0 8 6687.5 9 7732.5 10 6822.5 11 5889.5 12 6131.0 13 6390.5 14 6600.0 15 6924.5 16 7171.5 17 7370.0 18 7431.5 19 7740.5 20 8581.5 21 9766.5 22 11096.5 23 12769.0 24 14815.5 25 17598.0 26 22294.5 27 27269.0 28 30024.0 29 33645.0 30 39603.5 31 45576.0 32 52412.5 33 60665.0 34 69024.0 35 74664.5 36 82234.0 37 90725.5 38 92130.5 39 89941.0 40 88509.5 41 90081.0 42 91175.0 43 93397.0 44 96904.5 45 97275.0 46 96018.5 47 97837.5 48 119131.0 49 120747.0 50 97904.5 51 88092.0 52 81137.0 53 77321.5 54 73834.0 55 66493.5 56 58735.5 57 51773.0 58 45533.0 59 40295.0 60 34356.5 61 27229.5 62 21388.0 63 16543.0 64 12196.5 65 9137.0 66 6566.0 67 5063.0 68 3787.0 69 2406.5 70 1706.5 71 1248.5 72 912.0 73 708.0 74 540.0 75 443.0 76 319.5 77 194.5 78 112.5 79 67.0 80 44.0 81 37.0 82 27.0 83 20.0 84 19.0 85 18.5 86 15.5 87 11.5 88 7.5 89 6.0 90 8.0 91 5.0 92 4.5 93 3.0 94 2.0 95 3.5 96 2.5 97 2.5 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.325463997664397 2 0.08972655884727426 3 0.09875631994049638 4 0.10400286097094558 5 0.11599495475482949 6 0.058639910972979964 7 0.06038875798312969 8 0.06145948064240505 9 0.06452888559899438 10-11 0.061762852062533055 12-13 0.05999615967472873 14-15 0.05860422021767078 16-17 0.06067428402560312 18-19 0.06832995103942187 20-21 0.06970404511882523 22-23 0.08569350349733712 24-25 0.09491956374475971 26-27 0.08908412525170906 28-29 0.08765649503934192 30-31 0.08180321116863667 32-33 0.07264853243183243 34-35 0.32353669687770137 36-37 0.4994385844189866 38-39 0.47361632295279615 40-41 0.45486083103782293 42-43 0.4854121175824795 44-45 0.49379944508013646 46-47 0.4886242855603056 48-49 0.5070407152998416 50-51 0.5025258347532305 52-53 0.5022046179554479 54-55 0.493210547617535 56-57 0.4713856507459725 58-59 0.4554854192557335 60-61 0.41661818672403833 62-63 0.48559057135902545 64-65 0.5086824900440639 66-67 0.5194967889027448 68-69 0.5317030272184838 70-71 0.5291154474585684 72-73 0.516623683100356 74-75 0.5178014780255589 76-77 0.4918007627828225 78-79 0.4722422288733928 80-81 0.47841672954188064 82-83 0.48692897468311963 84-85 0.48944517293241674 86-87 0.4887313578262331 88-89 0.4946738685852113 90-91 0.5087717169323367 92-93 0.5165879923450468 94-95 0.5224055854604429 96-97 0.5138754949415492 98-99 0.5169627452757931 100-101 0.49417419801088286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2801846.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.57866960627532 #Duplication Level Percentage of deduplicated Percentage of total 1 67.76068174325431 26.141169532689794 2 15.068126104445732 11.626165171382084 3 6.283689506638914 7.272491441551253 4 3.2630983805215728 5.035439772596553 5 1.9325967004496847 3.7278504794413108 6 1.176049710162791 2.7222259925355696 7 0.7938375149761373 2.1437636647931724 8 0.5813807806039047 1.7943117640285184 9 0.46032659513202173 1.5982908862121936 >10 2.3158160161817762 16.203890336132325 >50 0.21438050170000444 5.775589509877176 >100 0.13842592660782804 10.056831199997106 >500 0.008252449759737261 2.1010892289924135 >1k 0.0028744487927154804 1.906312076106189 >5k 2.7817246381117553E-4 0.7755672087605465 >10k+ 1.8544830920745037E-4 1.1190117349037136 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17123 0.6111328031590602 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14159 0.5053454044226556 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9361 0.3341011604492181 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6683 0.23852131773123864 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5637 0.20118878767783813 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4189 0.1495085739901479 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2885 0.1029678290669794 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2877 0.10268230302450598 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.5690755309178306E-5 0.0 0.0 0.0 0.0 3 3.5690755309178306E-5 0.0 0.0 0.0 0.0 4 3.5690755309178306E-5 0.0 0.0 0.0 0.0 5 3.5690755309178306E-5 0.0 0.0 0.0 0.0 6 7.138151061835661E-5 0.0 0.0 0.0 0.0 7 7.138151061835661E-5 0.0 0.0 0.0 0.0 8 7.138151061835661E-5 0.0 0.0 0.0 0.0 9 7.138151061835661E-5 0.0 0.0 0.0 0.0 10-11 7.138151061835661E-5 0.0 0.0 0.0 0.0 12-13 1.0707226592753492E-4 0.0 0.0 0.0 0.0 14-15 2.3198990950965898E-4 0.0 0.0 0.0 0.0 16-17 2.498352871642481E-4 0.0 0.0 5.353613296376746E-5 0.0 18-19 2.8552604247342645E-4 0.0 0.0 7.138151061835661E-5 0.0 20-21 2.8552604247342645E-4 0.0 0.0 1.0707226592753492E-4 0.0 22-23 2.8552604247342645E-4 0.0 0.0 2.8552604247342645E-4 0.0 24-25 4.818251966739071E-4 0.0 0.0 7.851966168019227E-4 0.0 26-27 4.996705743284962E-4 0.0 0.0 0.0016596201218767913 0.0 28-29 6.067428402560312E-4 0.0 0.0 0.005942510758978188 0.0 30-31 6.424335955652095E-4 0.0 0.0 0.017113717170750997 0.0 32-33 6.602789732197986E-4 0.0 0.0 0.03836756195736668 0.0 34-35 7.495058614927445E-4 0.0 0.0 0.07200609883626724 0.0 36-37 8.20887372111101E-4 0.0 0.0 0.12638096454980038 0.0 38-39 8.744235050748685E-4 0.0 0.0 0.2050255438735748 0.0 40-41 8.922688827294577E-4 0.0 0.0 0.2964652589756896 0.0 42-43 9.45805015693225E-4 0.0 0.0 0.3893326042901716 0.0 44-45 0.0010707226592753492 0.0 0.0 0.5018834011576654 0.0 46-47 0.0012313310581666516 0.0 0.0 0.639721098161712 0.0 48-49 0.001284867191130419 0.0 0.0 0.7923347678637584 0.0 50-51 0.001284867191130419 0.0 0.0 0.9567620775731429 0.0 52-53 0.001284867191130419 0.0 0.0 1.1215998309685828 0.0 54-55 0.001284867191130419 0.0 0.0 1.3033371570029189 0.0 56-57 0.0013205579464395972 0.0 0.0 1.517463843480334 0.0 58-59 0.0013205579464395972 0.0 0.0 1.7599825258062007 0.0 60-61 0.0013384033240941865 0.0 0.0 2.0369070962501152 0.0 62-63 0.0013562487017487756 0.0 0.0 2.3122969642157347 0.0 64-65 0.0013562487017487756 0.0 0.0 2.590113803542379 0.0 66-67 0.001499011722985489 0.0 0.0 2.9089750114745776 0.0 68-69 0.001499011722985489 0.0 0.0 3.2768396264462787 0.0 70-71 0.001534702478294667 0.0 0.0 3.6945285358295923 0.0 72-73 0.0015703932336038455 0.0 0.0 4.121247206306128 0.0 74-75 0.0015703932336038455 0.0 0.0 4.5660075535914535 0.0 76-77 0.0015703932336038455 0.0 0.0 5.039838021076105 0.0 78-79 0.0015703932336038455 0.0 0.0 5.574342772586359 0.0 80-81 0.0018380738984226828 0.0 0.0 6.152247482552574 0.0 82-83 0.0018916100313864502 0.0 0.0 6.746766239115212 0.0 84-85 0.0019451461643502175 0.0 0.0 7.379973774432999 0.0 86-87 0.0020343730526231635 0.0 0.0 8.030098727767337 0.0 88-89 0.0020700638079323417 0.0 0.0 8.715932281788508 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13140 0.0 25.430077 1 TACATGG 13565 0.0 23.955044 2 GAGTACT 12315 0.0 23.81759 12-13 GTATCAA 25725 0.0 23.653913 1 CATGGGG 5205 0.0 23.206633 4 ACATGGG 13270 0.0 23.16682 3 GGTATCA 19260 0.0 22.943716 1 AGTACTT 13045 0.0 21.976627 12-13 GTACTTT 13875 0.0 21.14029 14-15 TATCAAC 30025 0.0 20.146544 2 ATCAACG 30040 0.0 20.089178 3 TCAACGC 30170 0.0 19.986914 4 CAACGCA 30165 0.0 19.943115 5 AACGCAG 30510 0.0 19.723614 6 CGCCGTT 65 0.007510226 18.292974 94-95 ACGCAGA 32640 0.0 18.175406 7 CATGGGA 7225 0.0 18.160784 4 CGCAGAG 32465 0.0 18.04004 8 ACTTTTT 16580 0.0 17.864862 16-17 ATGGGGG 2795 0.0 17.795088 5 >>END_MODULE