FastQCFastQC Report
Fri 27 May 2016
SRR939001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801846
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167140.5965352842376063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146570.5231194005666264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95950.34245279719156585No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA67370.24044861851793423No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA58250.2078986496759636No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT37080.13234132068643314No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31990.11417472623406141No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31130.11110532127747207No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG128800.024.710311
GGTATCA202150.023.8991131
TACATGG132900.023.7085842
GAGTACT131300.023.61969812-13
ACATGGG130850.022.4843543
CATGGGG51200.022.0581684
GTATCAA263250.021.8995191
GTACTTT146450.021.0142614-15
AGTACTT138450.020.96027612-13
CGCAGTA2251.6853173E-618.9811462
ATCAACG306550.018.63753
TCAACGC306600.018.634464
CAACGCA307800.018.5772275
TATCAAC310050.018.4424152
CATGGGA71400.018.4095884
AACGCAG311150.018.3924676
ACTTTTT172950.017.86202416-17
TACGCCG1650.001474293417.2555895
CGCAGAG332750.016.8705468
ACGCAGA335850.016.8702477
Produced by FastQC (version 0.11.5)