FastQCFastQC Report
Fri 27 May 2016
SRR939000_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939000_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819213
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171560.6085386240770031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140730.4991818638747764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91470.3244522496171804No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA70640.25056638146887095No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA57080.2024678518437592No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT40310.14298316586934012No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28740.10194334376295797No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28330.10048903718874735No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA260400.024.1088351
GTACATG133000.023.9934481
GAGTACT124900.023.51288412-13
GTACACG3300.022.9898031
TACATGG138100.022.6881242
ACATGGG135400.021.7772833
AGTACTT131250.021.72622712-13
CGTATAG2457.68523E-921.2890261
GGTATCA196200.021.146491
CATGGGG49200.020.971694
GTACTTT140900.020.87682314-15
ATCAACG305000.020.4371473
TATCAAC306350.020.4088822
TCAACGC306550.020.3341714
CAACGCA307100.020.2515185
AACGCAG309850.020.0696416
ACGCAGA332900.018.4671047
CGCAGAG330800.018.3986788
GTGTACG3105.7152647E-918.3547611
GTGTAAG11500.018.1419541
Produced by FastQC (version 0.11.5)