##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939000_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2819213 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.874117705898772 33.0 31.0 34.0 30.0 34.0 2 31.941498567153314 33.0 31.0 34.0 30.0 34.0 3 31.97217343989262 34.0 31.0 34.0 30.0 34.0 4 35.52480319862316 37.0 35.0 37.0 33.0 37.0 5 35.569883510043404 37.0 35.0 37.0 33.0 37.0 6 35.65506969498225 37.0 35.0 37.0 33.0 37.0 7 35.574562120705316 37.0 35.0 37.0 33.0 37.0 8 35.56449087032445 37.0 35.0 37.0 33.0 37.0 9 37.17782125720902 39.0 37.0 39.0 34.0 39.0 10-11 37.07524670892195 39.0 37.0 39.0 33.0 39.0 12-13 36.959635366323866 39.0 37.0 39.0 33.0 39.0 14-15 38.17089662966225 40.0 38.0 41.0 33.0 41.0 16-17 38.10780685957393 40.0 38.0 41.0 33.0 41.0 18-19 38.098568111029564 40.0 38.0 41.0 33.0 41.0 20-21 38.00858679354841 40.0 38.0 41.0 33.0 41.0 22-23 38.05864012403462 40.0 38.0 41.0 33.0 41.0 24-25 37.98802839657734 40.0 38.0 41.0 33.0 41.0 26-27 37.901089594862114 40.0 38.0 41.0 33.0 41.0 28-29 37.773235651226074 40.0 38.0 41.0 32.5 41.0 30-31 37.56987889882744 40.0 37.0 41.0 32.0 41.0 32-33 37.36010900914546 40.0 36.5 41.0 31.5 41.0 34-35 37.03938013906718 40.0 36.0 41.0 31.0 41.0 36-37 36.763982536970424 40.0 36.0 41.0 30.0 41.0 38-39 36.55680521478867 39.0 35.5 41.0 30.0 41.0 40-41 36.40115113685982 39.0 35.0 41.0 30.0 41.0 42-43 36.18134511298011 39.0 35.0 41.0 30.0 41.0 44-45 36.06701746196545 39.0 35.0 41.0 29.5 41.0 46-47 35.77908888047835 39.0 35.0 41.0 27.0 41.0 48-49 35.55749335009452 39.0 35.0 41.0 26.5 41.0 50-51 34.383083860637704 38.0 34.0 40.0 24.0 40.5 52-53 34.52519036340993 38.5 34.5 39.5 24.0 40.5 54-55 35.03648801988356 39.0 35.0 40.5 25.0 41.0 56-57 34.96301591969106 39.0 35.0 41.0 24.5 41.0 58-59 34.78122405082553 39.0 35.0 41.0 24.0 41.0 60-61 34.53444986242614 38.0 34.0 40.0 23.5 41.0 62-63 34.18942502748107 38.0 34.0 40.0 22.5 41.0 64-65 33.790014802003256 37.5 34.0 40.0 22.0 41.0 66-67 33.42676165298613 37.0 33.0 40.0 20.5 41.0 68-69 33.04412685384183 36.0 33.0 39.0 19.5 41.0 70-71 32.63470000315691 36.0 33.0 39.0 18.5 40.5 72-73 32.19740189904062 35.0 32.0 38.5 16.5 40.0 74-75 31.728779627505972 35.0 32.0 37.5 12.5 39.5 76-77 31.31137980705963 35.0 31.0 37.0 8.0 39.0 78-79 30.879156346115032 35.0 31.0 36.5 7.0 39.0 80-81 30.437861204527646 35.0 31.0 36.0 2.0 38.5 82-83 30.06412853516212 34.5 30.5 35.5 2.0 37.0 84-85 29.71147657165315 34.0 30.0 35.0 2.0 37.0 86-87 29.306752451836736 34.0 30.0 35.0 2.0 36.0 88-89 28.891400011279742 34.0 29.0 35.0 2.0 36.0 90-91 28.4655932347077 34.0 29.0 35.0 2.0 36.0 92-93 28.062593177599567 34.0 29.0 35.0 2.0 35.0 94-95 27.606229823713214 34.0 27.5 35.0 2.0 35.0 96-97 27.046496132076577 34.0 26.5 35.0 2.0 35.0 98-99 26.3690581378562 33.5 25.0 35.0 2.0 35.0 100-101 23.88173490261289 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28384848592879663 1101 2 0.3747796433844215 1101 3 0.3718039164925777 1101 4 0.31213085026961807 1101 5 0.14344303553998117 1101 6 0.2729991773110356 1101 7 0.26482899047720565 1101 8 0.3447265214552431 1101 9 0.2614202951861273 1101 10-11 0.36841811019937154 1101 12-13 0.4349970622736734 1101 14-15 0.24248328273440478 1101 16-17 0.4295745948407088 1101 18-19 0.3808085787772413 1101 20-21 0.5349838313907753 1101 22-23 0.466477743009321 1101 24-25 0.5170357084911288 1101 26-27 0.502925722781022 1101 28-29 0.4469023842543649 1101 30-31 0.4632982253200879 1101 32-33 0.5101729337119849 1101 34-35 0.5343943461164855 1101 36-37 0.5696381346155093 1101 38-39 0.5801391154805913 1101 40-41 0.5974115196466627 1101 42-43 0.6806916896043447 1101 44-45 0.6895563962146483 1101 46-47 0.6988092789273068 1101 48-49 0.7406711480466797 1101 50-51 0.5993997633485009 1101 52-53 0.5606124368692562 1101 54-55 0.5776369425045473 1101 56-57 0.5576158167148222 1101 58-59 0.49109485014490417 1101 60-61 0.45536864456178705 1101 62-63 0.5499957994249556 1101 64-65 0.586292936200401 1101 66-67 0.504992035087966 1101 68-69 0.578072025041763 1101 70-71 0.49425648520825405 1101 72-73 0.39574778518945664 1101 74-75 0.3091788257817072 1101 76-77 0.3740887565329949 1101 78-79 0.29207713449094896 1101 80-81 0.19702405299445047 1101 82-83 0.23262143787573208 1101 84-85 0.11162721732338454 1101 86-87 -0.039801187096831114 1101 88-89 -0.04974700113800523 1101 90-91 -0.06758978040024388 1101 92-93 0.04302145635722354 1101 94-95 -0.055077661079803164 1101 96-97 -0.08190871982055015 1101 98-99 -0.3140496820011869 1101 100-101 0.07042709982349038 1102 1 0.19675211270653747 1102 2 0.19895104756469095 1102 3 0.14161938799245277 1102 4 0.13042726852170716 1102 5 0.27019732275525854 1102 6 0.24146656820749968 1102 7 0.3595745719249166 1102 8 0.2701270993380902 1102 9 0.2930678241740807 1102 10-11 0.285274160144958 1102 12-13 0.3282230041476666 1102 14-15 0.34772292587298637 1102 16-17 0.37761849423655747 1102 18-19 0.4135877168312092 1102 20-21 0.5069140632944098 1102 22-23 0.5231525947861257 1102 24-25 0.5182236048474635 1102 26-27 0.5862350167507699 1102 28-29 0.5726417412658904 1102 30-31 0.46642803319280546 1102 32-33 0.47565827087284873 1102 34-35 0.5222069751081975 1102 36-37 0.44357747098835176 1102 38-39 0.47808791280882446 1102 40-41 0.4711620590665362 1102 42-43 0.36486194458576904 1102 44-45 0.6482925354694018 1102 46-47 0.7096979379764363 1102 48-49 0.7721808904385199 1102 50-51 0.6562839334862147 1102 52-53 0.6227700225197523 1102 54-55 0.7475407146911195 1102 56-57 0.6185075246588383 1102 58-59 0.5545001373977101 1102 60-61 0.5872175178778605 1102 62-63 0.5382540361317822 1102 64-65 0.6761086891367185 1102 66-67 0.6311007774422777 1102 68-69 0.5785962004329619 1102 70-71 0.5691725233335063 1102 72-73 0.5051931443023676 1102 74-75 0.550679444941899 1102 76-77 0.4739242334739693 1102 78-79 0.4047906743986367 1102 80-81 0.33062955882956757 1102 82-83 0.2104882199313458 1102 84-85 0.21422956637963253 1102 86-87 0.31521506585825065 1102 88-89 0.28813915312669636 1102 90-91 0.3438849700594311 1102 92-93 0.3302988870300432 1102 94-95 0.239178220942037 1102 96-97 0.10552971595565097 1102 98-99 -0.09055278718336979 1102 100-101 0.11889925168659943 1103 1 0.02426410554481606 1103 2 0.1913991332803846 1103 3 0.26164883401354544 1103 4 0.21933509415626418 1103 5 0.13401006105945612 1103 6 0.18707022921618943 1103 7 0.2302370546709298 1103 8 0.09689796172100529 1103 9 0.23638745587032162 1103 10-11 0.2730280778835805 1103 12-13 0.29742903372726914 1103 14-15 0.2417506447378841 1103 16-17 0.40792369937443596 1103 18-19 0.3327992243315592 1103 20-21 0.33098653706483816 1103 22-23 0.2975719644795731 1103 24-25 0.3635782401644079 1103 26-27 0.2493168188050774 1103 28-29 0.21810411878895053 1103 30-31 0.2764678023116218 1103 32-33 0.31209385521173516 1103 34-35 0.3455917794145975 1103 36-37 0.26981562754791355 1103 38-39 0.36739627111991524 1103 40-41 0.46014689782231954 1103 42-43 0.23281651826878402 1103 44-45 0.3427540353534013 1103 46-47 0.39694264343419405 1103 48-49 0.36665290675862394 1103 50-51 0.1716572640386005 1103 52-53 0.1659439473825941 1103 54-55 0.31863899972110943 1103 56-57 0.4553946712890067 1103 58-59 0.3981280559284386 1103 60-61 0.3146309081425116 1103 62-63 0.28011522269042644 1103 64-65 0.20914172304944145 1103 66-67 0.2176157476916032 1103 68-69 0.11842244195829466 1103 70-71 0.12500396038839767 1103 72-73 0.22949027141122258 1103 74-75 0.07068864902051786 1103 76-77 -0.046463152192742996 1103 78-79 -0.02302190103262447 1103 80-81 -0.05907367847513356 1103 82-83 -0.09356889808474733 1103 84-85 -0.0785029025163233 1103 86-87 0.07851806459510513 1103 88-89 -0.06718500503113134 1103 90-91 -0.18472544841855765 1103 92-93 -0.17450718285767763 1103 94-95 -0.20163738580066948 1103 96-97 -0.3872318122846359 1103 98-99 -0.44267836690557516 1103 100-101 -0.23835125978129312 1104 1 0.22585182147660632 1104 2 0.15992918941774548 1104 3 0.17779198602698543 1104 4 0.11784384091347277 1104 5 0.10842670381213537 1104 6 0.18563481369480428 1104 7 0.16366224076785585 1104 8 0.16257931445751694 1104 9 0.24861096118844728 1104 10-11 0.16099911141733259 1104 12-13 0.16957793892760975 1104 14-15 0.22199769064007313 1104 16-17 0.1319954620831325 1104 18-19 0.22665194643489173 1104 20-21 0.20086678272776481 1104 22-23 0.07673680158210061 1104 24-25 0.10470975211621436 1104 26-27 0.05424004625995593 1104 28-29 0.10717389922972842 1104 30-31 0.141997430878412 1104 32-33 0.25500871680448967 1104 34-35 0.2781678748769352 1104 36-37 0.19330685658859892 1104 38-39 0.17378358206975264 1104 40-41 0.23148998806276921 1104 42-43 0.2643597887573108 1104 44-45 0.35980168354233655 1104 46-47 0.3991956004132149 1104 48-49 0.4183470255310482 1104 50-51 0.24861881610828362 1104 52-53 0.278720586124642 1104 54-55 0.2912737622465684 1104 56-57 0.21539259106017283 1104 58-59 0.25322254211191364 1104 60-61 0.2886665117488576 1104 62-63 0.20547073437485608 1104 64-65 0.2601081939095238 1104 66-67 0.37884414298810043 1104 68-69 0.2769125415672562 1104 70-71 0.2493670703823625 1104 72-73 0.21135929075332172 1104 74-75 0.3510219581517475 1104 76-77 0.15910768334471115 1104 78-79 0.13740235040192417 1104 80-81 0.07582239075672703 1104 82-83 -0.010945349202824417 1104 84-85 0.020896164895770397 1104 86-87 0.17419202416375512 1104 88-89 0.3925789898380252 1104 90-91 0.22306912869569473 1104 92-93 0.2628233303537897 1104 94-95 0.24447806056112142 1104 96-97 0.1791748834015081 1104 98-99 0.02999442750443393 1104 100-101 0.3918827342696005 1105 1 0.05608927859906032 1105 2 0.07268671437884677 1105 3 0.11916962223734018 1105 4 0.07710320344454402 1105 5 0.08224748251316782 1105 6 0.13368751439232085 1105 7 0.14634501637874564 1105 8 0.14407649932875444 1105 9 0.29821687596587765 1105 10-11 0.10511752068642721 1105 12-13 0.14258517876739774 1105 14-15 0.21964755838532568 1105 16-17 0.21504203390254162 1105 18-19 0.25477535065763135 1105 20-21 0.3125998938687573 1105 22-23 0.31210581362444145 1105 24-25 0.3301755140489391 1105 26-27 0.22159204287458323 1105 28-29 0.37751988834099137 1105 30-31 0.3468730873681878 1105 32-33 0.23456349563455348 1105 34-35 0.289872063836647 1105 36-37 0.26842730189178354 1105 38-39 0.13474752174103344 1105 40-41 0.08129064917243767 1105 42-43 0.06859885655549647 1105 44-45 0.13689435917761017 1105 46-47 0.20923878686123487 1105 48-49 0.09658352149670435 1105 50-51 0.12846082117540192 1105 52-53 0.1768617482386574 1105 54-55 0.20083112165688277 1105 56-57 0.08395918260570312 1105 58-59 0.22314812080156798 1105 60-61 0.257953916220913 1105 62-63 0.10797482667215519 1105 64-65 0.059428440639244684 1105 66-67 0.002526652886700731 1105 68-69 -0.013369933559125968 1105 70-71 -0.12780441683009514 1105 72-73 -0.0760193481655449 1105 74-75 -0.11316565659386413 1105 76-77 -0.19987877735665904 1105 78-79 -0.04974794543536021 1105 80-81 -0.200943611847908 1105 82-83 -0.07636866277722376 1105 84-85 -0.013473255009468943 1105 86-87 -0.01828331543951478 1105 88-89 -0.036808789364904726 1105 90-91 -0.01669165370124759 1105 92-93 -0.027614827024425637 1105 94-95 0.04258483316279538 1105 96-97 0.010967932892882004 1105 98-99 0.05242456321452238 1105 100-101 0.33141993016297633 1106 1 0.14266137438043458 1106 2 0.1375945039322346 1106 3 0.14124741281386477 1106 4 0.08198385213908921 1106 5 0.15119533669066954 1106 6 0.13486293089717094 1106 7 0.12514535129049165 1106 8 0.06449979732116162 1106 9 0.05439871813382524 1106 10-11 0.11023141526747793 1106 12-13 0.07395503866652575 1106 14-15 0.12040503580298179 1106 16-17 0.21247593258672026 1106 18-19 0.1387871746179954 1106 20-21 0.152336242077574 1106 22-23 0.10222437047946187 1106 24-25 0.2571842905450481 1106 26-27 0.17806812251421178 1106 28-29 0.037438595308806555 1106 30-31 0.014767230616193672 1106 32-33 0.12401505782018774 1106 34-35 0.10262254374551105 1106 36-37 0.05383996270850133 1106 38-39 0.10107980981906906 1106 40-41 0.17524399576512195 1106 42-43 0.20220704418009916 1106 44-45 0.19408626376741722 1106 46-47 0.15013577294632086 1106 48-49 0.19999882554649417 1106 50-51 0.03250284218326982 1106 52-53 -0.026894032677027724 1106 54-55 0.09848693770815942 1106 56-57 0.1300247494850808 1106 58-59 0.2677887000603576 1106 60-61 0.1650008369125544 1106 62-63 -0.034895383921984546 1106 64-65 -0.1512013192886812 1106 66-67 -0.09745929551922927 1106 68-69 0.06200259230943317 1106 70-71 -6.26717172536928E-4 1106 72-73 -0.04563247856724928 1106 74-75 0.06340438762833855 1106 76-77 0.06128807600365249 1106 78-79 0.10013510776056478 1106 80-81 0.1047643492327417 1106 82-83 0.13059704155132934 1106 84-85 0.09914657019028539 1106 86-87 0.37391841997861874 1106 88-89 0.3457206773433903 1106 90-91 0.21254457144044991 1106 92-93 0.2791750935807613 1106 94-95 0.3833417395278893 1106 96-97 0.46572087467275125 1106 98-99 0.4654061343440077 1106 100-101 0.7863764416029326 1107 1 0.0659649186868414 1107 2 -0.052770491180769596 1107 3 0.03540956371503157 1107 4 0.11843458603415513 1107 5 0.10382758492720967 1107 6 0.07223908717944738 1107 7 0.07538671352578774 1107 8 0.10311088118683642 1107 9 0.07985550069155778 1107 10-11 0.1058490495818134 1107 12-13 0.1345383609180928 1107 14-15 0.08541200608101462 1107 16-17 0.06105520142265419 1107 18-19 0.12712355841184575 1107 20-21 0.1211222055000718 1107 22-23 0.11532454076416343 1107 24-25 0.20325525069853967 1107 26-27 0.1817237180757516 1107 28-29 0.15860986639513186 1107 30-31 0.07529298495413883 1107 32-33 0.17293218619782635 1107 34-35 -0.104970657450842 1107 36-37 -0.07356245670369077 1107 38-39 -0.04722029098756764 1107 40-41 -0.15115265733816585 1107 42-43 -0.11976983400777641 1107 44-45 -0.10140117849612551 1107 46-47 -0.009122588413084998 1107 48-49 -0.12836161383614098 1107 50-51 -0.10507977867828089 1107 52-53 -0.12361374554334503 1107 54-55 -0.11006865888445105 1107 56-57 -0.02869626728456609 1107 58-59 -0.07092649514576976 1107 60-61 -0.1250014605164722 1107 62-63 -0.19250066710985436 1107 64-65 -0.20721384173091195 1107 66-67 -0.3039839542822875 1107 68-69 -0.3745623256586086 1107 70-71 -0.2395454556011245 1107 72-73 -0.30261042351302336 1107 74-75 -0.29495058709861866 1107 76-77 -0.18890584392579157 1107 78-79 -0.12655847945145027 1107 80-81 -0.19527426290862593 1107 82-83 -0.11477392978527234 1107 84-85 -0.10399995581415311 1107 86-87 -0.1005803161710439 1107 88-89 -0.12367784569262952 1107 90-91 -0.026750176012878768 1107 92-93 -0.0760786163441054 1107 94-95 -0.2182052180442291 1107 96-97 -0.14320178181084842 1107 98-99 -0.011584215132224074 1107 100-101 0.3078281232866047 1108 1 0.13063356043645413 1108 2 0.18663511792858856 1108 3 0.08716841068486403 1108 4 0.22904811025912153 1108 5 0.2643279993701029 1108 6 0.2976451803689244 1108 7 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2316 78-79 -1.54175864826259 2316 80-81 -1.5102870861428705 2316 82-83 -1.5812314125756934 2316 84-85 -1.6297191371471946 2316 86-87 -1.6548534842737013 2316 88-89 -1.9311605942044778 2316 90-91 -2.2308783270510695 2316 92-93 -2.434648526597183 2316 94-95 -2.3595974845411902 2316 96-97 -2.503032117817515 2316 98-99 -2.941859044524218 2316 100-101 -3.7337819045475342 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8816.0 3 2005.0 4 1887.0 5 2249.0 6 2557.0 7 3113.0 8 4000.0 9 4821.0 10 6193.0 11 8499.0 12 11868.0 13 18263.0 14 17537.0 15 17068.0 16 22345.0 17 30894.0 18 25822.0 19 16898.0 20 16174.0 21 16964.0 22 18015.0 23 20139.0 24 22791.0 25 26492.0 26 30852.0 27 35360.0 28 42389.0 29 50412.0 30 61434.0 31 75140.0 32 92496.0 33 119069.0 34 160141.0 35 230188.0 36 349026.0 37 565642.0 38 576687.0 39 104942.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00690382351654 20.18928151783799 14.698915103283433 22.104899555362035 2 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20.14289386802335 20-21 21.364563380091646 27.165780224162216 31.14437635276654 20.325280042979603 22-23 21.201142311460103 27.05743288174399 30.82674818559596 20.914676621199952 24-25 21.09551378391371 27.209868492670964 31.130990022718503 20.563627700696827 26-27 20.88780408625436 27.26686032335351 31.24883345140152 20.596502138990612 28-29 20.504527618445884 27.325193511227457 31.56609445695482 20.60418441337184 30-31 20.72626777599582 27.497908288575207 31.24894349040938 20.526880445019597 32-33 20.589919989602308 27.642099155222873 30.914283299520704 20.85369755565412 34-35 21.10598858452448 27.372339709567346 30.810193238383604 20.71147846752458 36-37 21.29899078671607 27.0457813200539 30.815697033697592 20.83953085953243 38-39 20.993419739426034 27.459124664266504 30.95005734025757 20.597398256049892 40-41 20.848500561872484 27.319146067944665 31.17966117894435 20.652692191238497 42-43 20.66376731615186 27.629699798967028 30.56965763037936 21.13687525450175 44-45 20.790929851819065 27.888507766738975 30.233862949806944 21.08669943163502 46-47 20.984469914673898 27.860629159143507 29.713154889509884 21.441746036672708 48-49 21.184761867378683 27.925930749872208 29.599170704161022 21.29013667858809 50-51 21.17283259021597 28.173883040368114 29.493082871382654 21.16020149803326 52-53 21.138242422674573 28.3645715600856 29.50486570580611 20.99232031143372 54-55 20.70198272872452 28.12001838159215 29.662295329962422 21.515703559720908 56-57 20.987792591947915 28.79704535390319 29.071979103893604 21.14318295025529 58-59 21.09693090966677 27.996227146603957 29.35590601536141 21.55093592836786 60-61 21.138603802237995 28.074782813285378 29.628652604636407 21.157960779840224 62-63 21.22154743564154 27.938964902714254 29.70591398798869 21.133573673655516 64-65 20.907013285855555 27.872746420341787 29.777799427797124 21.442440866005533 66-67 20.701182884965846 28.30814827928106 29.348321111402193 21.6423477243509 68-69 20.67041658420334 28.16407099775658 29.605830354987 21.55968206305308 70-71 20.823981613553777 28.186394222524324 29.302836540117383 21.686787623804516 72-73 20.924130370156774 28.034501476223223 29.083132084238763 21.958236069381243 74-75 20.804963879688653 28.399704085736627 29.033196855429182 21.762135179145538 76-77 21.04086250784088 27.893419652624797 29.318230311801326 21.747487527732996 78-79 20.79767043945556 28.06281437554033 29.334130733850454 21.80538445115366 80-81 20.78679801071974 28.41821043252078 29.02562792811367 21.769363628645806 82-83 20.867857268997188 28.27342207850355 29.008222241328685 21.850498411170573 84-85 20.78949969262189 27.86684944038631 28.9903583532811 22.353292513710706 86-87 20.88794824181646 27.88566678617998 29.278639046604845 21.94774592539872 88-89 21.210170685910274 27.56136352486252 29.18553339904466 22.04293239018255 90-91 20.741114115622437 27.690789690848572 29.51613291736067 22.05196327616832 92-93 20.933407589194022 27.808688970667088 29.300468036759863 21.957435403379023 94-95 20.892010066821882 27.5273404432999 29.135255432043955 22.445394057834264 96-97 21.05038235934909 27.227979029610033 29.007064775279908 22.71457383576097 98-99 20.826087833216427 27.303007305605426 28.987002799580946 22.883902061597205 100-101 21.16180040957161 26.93712046432626 29.584350289873868 22.31672883622827 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4981.0 1 3082.0 2 1365.5 3 1566.5 4 1398.5 5 1415.0 6 2236.0 7 4172.0 8 6623.0 9 7822.0 10 6934.5 11 5919.0 12 6077.5 13 6460.5 14 6824.5 15 7074.5 16 7335.5 17 7504.5 18 7584.0 19 7932.0 20 8648.5 21 9907.0 22 11319.0 23 12999.5 24 14972.5 25 17721.0 26 22491.0 27 27204.0 28 29918.5 29 33697.5 30 39815.5 31 45803.0 32 52708.0 33 61102.0 34 69550.0 35 75427.0 36 82516.0 37 91272.5 38 92576.0 39 90013.5 40 89028.5 41 90604.0 42 91935.0 43 94366.5 44 97952.0 45 98329.5 46 96623.5 47 98160.0 48 120096.0 49 121836.0 50 98167.0 51 88391.0 52 81971.5 53 78010.0 54 74139.0 55 66498.5 56 58660.5 57 51965.0 58 45978.0 59 40409.5 60 34248.5 61 27325.5 62 21313.5 63 16425.5 64 12223.0 65 9111.5 66 6508.5 67 4917.0 68 3599.0 69 2368.5 70 1718.5 71 1258.0 72 973.5 73 735.0 74 524.5 75 451.0 76 331.5 77 194.5 78 114.5 79 61.0 80 42.0 81 32.0 82 22.0 83 18.5 84 18.5 85 17.5 86 15.0 87 11.0 88 6.0 89 4.5 90 3.0 91 4.0 92 5.0 93 3.5 94 2.0 95 0.5 96 0.5 97 1.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28951342094407195 2 0.08867723013479294 3 0.09307562074947867 4 0.1077960409518543 5 0.10971146912276582 6 0.08498825736118555 7 0.09591329211379204 8 0.092508086476616 9 0.09804154563702706 10-11 0.0903266266153001 12-13 0.09476048812203973 14-15 0.10423121630043562 16-17 0.09202922943388811 18-19 0.10382330104181557 20-21 0.10618211536340105 22-23 0.11859692758227208 24-25 0.13005402571568733 26-27 0.13299810975616244 28-29 0.125903931345379 30-31 0.11836636678392162 32-33 0.11364873814075063 34-35 0.3873421412287755 36-37 0.5917786275815272 38-39 0.5749299538559165 40-41 0.5390156756513254 42-43 0.5654592256775206 44-45 0.5861387557449543 46-47 0.574699393057566 48-49 0.5971702031737226 50-51 0.5889941625552947 52-53 0.5920269238259046 54-55 0.5820418677127269 56-57 0.5543568364646445 58-59 0.5497810913896892 60-61 0.4974083192720805 62-63 0.5712409810823091 64-65 0.605133418439827 66-67 0.6178674686871832 68-69 0.6194104524915286 70-71 0.6160052468543527 72-73 0.6076163808836011 74-75 0.6047787095192878 76-77 0.5886394536347556 78-79 0.565317342109305 80-81 0.5599789728551905 82-83 0.5748412766257818 84-85 0.5844184174803394 86-87 0.573848091648272 88-89 0.5870609989383562 90-91 0.6010720012996535 92-93 0.6097268989608093 94-95 0.6078646771279786 96-97 0.6055413336984471 98-99 0.6096204862846475 100-101 0.58628063931317 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2819213.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.9088249705986 #Duplication Level Percentage of deduplicated Percentage of total 1 68.34541591800695 26.592398254964962 2 14.726850585687817 11.460089036133697 3 6.162692394480533 7.19349359173447 4 3.226553502039838 5.021656218765602 5 1.8958080764139784 3.6881832311519362 6 1.192994416029139 2.7850806554507566 7 0.8171846683389201 2.2256986660339 8 0.575553707376166 1.7915294769182695 9 0.4303342888935616 1.5069421372865945 >10 2.2697207017721723 16.06836349282603 >50 0.20815925932759569 5.665966729072616 >100 0.13736881699964862 10.116444203469555 >500 0.008247821105191833 2.149404146001752 >1k 0.002657631245004317 1.8396517601958418 >5k 2.7492737017286036E-4 0.7815658506334706 >10k+ 1.832849134485736E-4 1.1135325493604935 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17156 0.6085386240770031 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14073 0.4991818638747764 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9147 0.3244522496171804 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7064 0.25056638146887095 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5708 0.2024678518437592 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4031 0.14298316586934012 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2874 0.10194334376295797 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2833 0.10048903718874735 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.5470892053917175E-5 0.0 0.0 0.0 0.0 3 3.5470892053917175E-5 0.0 0.0 0.0 0.0 4 3.5470892053917175E-5 0.0 0.0 0.0 0.0 5 3.5470892053917175E-5 0.0 0.0 0.0 0.0 6 3.5470892053917175E-5 0.0 0.0 0.0 0.0 7 3.5470892053917175E-5 0.0 0.0 0.0 0.0 8 1.0641267616175152E-4 0.0 0.0 0.0 0.0 9 1.0641267616175152E-4 0.0 0.0 0.0 0.0 10-11 1.0641267616175152E-4 0.0 0.0 0.0 0.0 12-13 1.596190142426273E-4 0.0 0.0 0.0 0.0 14-15 1.9508990629654445E-4 0.0 0.0 0.0 0.0 16-17 2.3056079835046163E-4 0.0 0.0 0.0 0.0 18-19 3.192380284852546E-4 0.0 0.0 0.0 0.0 20-21 3.5470892053917176E-4 0.0 0.0 3.5470892053917175E-5 0.0 22-23 3.9017981259308895E-4 0.0 0.0 1.9508990629654445E-4 0.0 24-25 4.256507046470061E-4 0.0 0.0 4.965924887548404E-4 0.0 26-27 4.256507046470061E-4 0.0 0.0 0.0013478938980488525 0.0 28-29 5.675342728626748E-4 0.0 0.0 0.00590590352697721 0.0 30-31 6.384760569705092E-4 0.0 0.0 0.018161096731605594 0.0 32-33 6.384760569705092E-4 0.0 0.0 0.0404722878335195 0.0 34-35 7.094178410783435E-4 0.0 0.0 0.07539338106060095 0.0 36-37 7.803596251861779E-4 0.0 0.0 0.1322886919150841 0.0 38-39 8.513014092940121E-4 0.0 0.0 0.21449248425003714 0.0 40-41 8.513014092940121E-4 0.0 0.0 0.3050674071097147 0.0 42-43 9.222431934018465E-4 0.0 0.0 0.3997037471095657 0.0 44-45 9.577140854557637E-4 0.0 0.0 0.5192406533312666 0.0 46-47 0.0010463913155905565 0.0 0.0 0.6611419569929622 0.0 48-49 0.0011350685457253496 0.0 0.0 0.8210802092640748 0.0 50-51 0.0011350685457253496 0.0 0.0 0.9883432007443211 0.0 52-53 0.0011528039917523083 0.0 0.0 1.1547903617073274 0.0 54-55 0.0011705394377792668 0.0 0.0 1.3384586407625108 0.0 56-57 0.0011705394377792668 0.0 0.0 1.5559306799450767 0.0 58-59 0.0011705394377792668 0.0 0.0 1.8086785212752636 0.0 60-61 0.001206010329833184 0.0 0.0 2.0955493607613187 0.0 62-63 0.0012414812218871011 0.0 0.0 2.370927631221905 0.0 64-65 0.0012414812218871011 0.0 0.0 2.657284142773178 0.0 66-67 0.0013124230059949355 0.0 0.0 2.9865072273716105 0.0 68-69 0.0013478938980488527 0.0 0.0 3.358472736895013 0.0 70-71 0.0013833647901027699 0.0 0.0 3.7756636337871594 0.0 72-73 0.0014365711281836458 0.0 0.0 4.205091988437908 0.0 74-75 0.0014543065742106042 0.0 0.0 4.6564768252700315 0.0 76-77 0.0014543065742106042 0.0 0.0 5.13978546495068 0.0 78-79 0.0014543065742106042 0.0 0.0 5.672114877449841 0.0 80-81 0.0015252483583184386 0.0 0.0 6.254546924975162 0.0 82-83 0.0015252483583184386 0.0 0.0 6.849766229085919 0.0 84-85 0.001542983804345397 0.0 0.0 7.476377272664393 0.0 86-87 0.001596190142426273 0.0 0.0 8.13549738881028 0.0 88-89 0.00163166103448019 0.0 0.0 8.830478576822681 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 26040 0.0 24.108835 1 GTACATG 13300 0.0 23.993448 1 GAGTACT 12490 0.0 23.512884 12-13 GTACACG 330 0.0 22.989803 1 TACATGG 13810 0.0 22.688124 2 ACATGGG 13540 0.0 21.777283 3 AGTACTT 13125 0.0 21.726227 12-13 CGTATAG 245 7.68523E-9 21.289026 1 GGTATCA 19620 0.0 21.14649 1 CATGGGG 4920 0.0 20.97169 4 GTACTTT 14090 0.0 20.876823 14-15 ATCAACG 30500 0.0 20.437147 3 TATCAAC 30635 0.0 20.408882 2 TCAACGC 30655 0.0 20.334171 4 CAACGCA 30710 0.0 20.251518 5 AACGCAG 30985 0.0 20.069641 6 ACGCAGA 33290 0.0 18.467104 7 CGCAGAG 33080 0.0 18.398678 8 GTGTACG 310 5.7152647E-9 18.354761 1 GTGTAAG 1150 0.0 18.141954 1 >>END_MODULE