FastQCFastQC Report
Fri 27 May 2016
SRR939000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819213
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166660.5911578869705837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146730.5204643991071267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99940.3544960951868482No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA69180.245387631228999No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA57520.2040285710941316No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT37570.13326414144656681No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31600.11208801889037827No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30860.10946317287838839No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29690.1053130785080801No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG126100.024.6786921
GGTATCA198900.024.6549761
TACATGG130900.023.3096162
ACATGGG127200.022.7565543
GAGTACT135550.022.59830712-13
TACCGTA1050.002209680422.5966722
GGACCGA3650.022.1014026
GTATCAA257000.021.3064421
CGTATAG3151.2732926E-1121.1807581
CATGGGG48200.020.8714474
GTACTTT148800.020.61791614-15
AGTACTT142000.020.40217812-13
CATGGGA72050.019.2314764
ATCAACG301850.017.984513
TCAACGC302150.017.9666524
CAACGCA304600.017.8377195
TATCAAC305450.017.8036172
AACGCAG307500.017.6849276
ACTTTTT176200.017.51898816-17
AGTACAT100150.017.1522622
Produced by FastQC (version 0.11.5)