##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939000_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2819213 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15385499428387 33.0 31.0 34.0 30.0 34.0 2 32.37717263647692 34.0 31.0 34.0 30.0 34.0 3 32.4617118323447 34.0 31.0 34.0 30.0 34.0 4 36.04910838592188 37.0 35.0 37.0 35.0 37.0 5 35.97234689255477 37.0 35.0 37.0 35.0 37.0 6 36.01713669736909 37.0 35.0 37.0 35.0 37.0 7 35.958654418804116 37.0 35.0 37.0 35.0 37.0 8 35.93062106339606 37.0 35.0 37.0 35.0 37.0 9 37.599957505871316 39.0 37.0 39.0 35.0 39.0 10-11 37.529003661660184 39.0 37.0 39.0 35.0 39.0 12-13 37.45554823278695 39.0 37.0 39.0 34.0 39.0 14-15 38.69402223244572 40.0 38.0 41.0 34.0 41.0 16-17 38.686016097400234 40.0 38.0 41.0 34.0 41.0 18-19 38.814899406323676 40.0 38.0 41.0 34.5 41.0 20-21 38.82506217160605 40.0 38.0 41.0 34.0 41.0 22-23 38.7633380308618 40.0 38.0 41.0 34.0 41.0 24-25 38.70120916723923 40.0 38.0 41.0 34.0 41.0 26-27 38.53534266477914 40.0 38.0 41.0 34.0 41.0 28-29 38.350028891041575 40.0 38.0 41.0 34.0 41.0 30-31 38.154485666744584 40.0 37.5 41.0 33.5 41.0 32-33 37.9659905086987 40.0 37.0 41.0 33.0 41.0 34-35 37.910600759857445 40.0 37.0 41.0 33.0 41.0 36-37 37.78368714956976 40.0 37.0 41.0 33.0 41.0 38-39 37.64522669979175 40.0 37.0 41.0 33.0 41.0 40-41 37.47394414682395 40.0 37.0 41.0 32.5 41.0 42-43 37.28985163589981 40.0 37.0 41.0 32.0 41.0 44-45 37.01297347876872 40.0 36.0 41.0 31.0 41.0 46-47 36.6439953277741 40.0 36.0 41.0 30.5 41.0 48-49 36.303928436765865 39.5 36.0 41.0 30.0 41.0 50-51 36.03802976220669 39.0 35.0 41.0 29.0 41.0 52-53 35.806823570975304 39.0 35.0 41.0 28.0 41.0 54-55 35.56543315457186 39.0 35.0 40.0 28.0 41.0 56-57 35.32065047940684 38.5 35.0 40.0 27.0 41.0 58-59 35.037822966906006 38.0 34.5 40.0 26.5 41.0 60-61 34.64639848071075 38.0 34.0 40.0 26.0 41.0 62-63 34.337793384182035 37.5 34.0 40.0 26.0 41.0 64-65 34.090679029927855 37.0 34.0 40.0 25.5 41.0 66-67 33.65736962762303 36.5 33.0 39.0 24.0 41.0 68-69 33.24955865342562 36.0 33.0 39.0 23.0 40.0 70-71 32.807202045393524 35.5 32.5 38.5 22.0 40.0 72-73 32.2992991306439 35.0 32.0 38.0 21.0 40.0 74-75 31.822024444410552 35.0 31.5 37.0 19.5 39.0 76-77 29.82301035785519 33.0 29.0 35.5 16.0 37.5 78-79 30.799627768458784 34.0 30.5 36.0 17.5 38.5 80-81 30.79492397346352 34.0 31.0 36.0 16.5 37.5 82-83 30.466768030652524 34.0 31.0 35.0 12.0 37.0 84-85 30.069579169789584 34.0 30.5 35.0 7.5 36.5 86-87 29.49943246572714 34.0 29.5 35.0 2.0 36.0 88-89 28.93075833574831 34.0 29.0 35.0 2.0 36.0 90-91 28.47361976551612 34.0 29.0 35.0 2.0 35.0 92-93 28.13296370299087 34.0 29.0 35.0 2.0 35.0 94-95 27.76859942828016 33.0 27.5 35.0 2.0 35.0 96-97 27.173584613862097 33.0 27.0 35.0 2.0 35.0 98-99 26.313685769752055 33.0 25.5 34.5 2.0 35.0 100-101 22.042813012000156 28.5 8.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17203566794225367 1101 2 0.16559210597765173 1101 3 0.14683151720980447 1101 4 0.15854191250120664 1101 5 0.21913107023191714 1101 6 0.15680086553834371 1101 7 0.1602305419952188 1101 8 0.21000493622868532 1101 9 0.16607647110430435 1101 10-11 0.24037156528774517 1101 12-13 0.24880114762054006 1101 14-15 0.31075440785448905 1101 16-17 0.32687734386689016 1101 18-19 0.35125604094520213 1101 20-21 0.36986206349568107 1101 22-23 0.3555448097000564 1101 24-25 0.32260265199668936 1101 26-27 0.32679802902852373 1101 28-29 0.3384475452503324 1101 30-31 0.3435198187450226 1101 32-33 0.43408150577579363 1101 34-35 0.3522222883264092 1101 36-37 0.44644096814401735 1101 38-39 0.44340026080627837 1101 40-41 0.3477474439729207 1101 42-43 0.27415822978652926 1101 44-45 0.4227541933416106 1101 46-47 0.4178212780099244 1101 48-49 0.296832930591556 1101 50-51 0.22976064740972646 1101 52-53 0.30691270849154506 1101 54-55 0.3637885027345362 1101 56-57 0.17753462226567507 1101 58-59 0.3099982235518084 1101 60-61 0.41655750735123576 1101 62-63 0.24659608830064172 1101 64-65 0.21085724741306677 1101 66-67 0.3056659462097926 1101 68-69 0.21413029608158496 1101 70-71 0.3064108352393973 1101 72-73 0.19542575323850997 1101 74-75 0.08143052759667313 1101 76-77 -0.03237801474133306 1101 78-79 0.10233026156531011 1101 80-81 0.23391639551224586 1101 82-83 0.3213357269085151 1101 84-85 0.30677531777756073 1101 86-87 0.2991021302475758 1101 88-89 0.4599879992001128 1101 90-91 0.5005054627254317 1101 92-93 0.46482536823151577 1101 94-95 0.46530393287660843 1101 96-97 0.37324935267610115 1101 98-99 0.18115907213949 1101 100-101 0.8722883658959653 1102 1 0.12764258565023567 1102 2 0.13842058922280387 1102 3 0.1487663928148919 1102 4 0.13393498541201154 1102 5 0.078076293317217 1102 6 0.14096889248119027 1102 7 0.0600602729419748 1102 8 0.1464073340672556 1102 9 0.22133534573374902 1102 10-11 0.21532548006879182 1102 12-13 0.23131632173589622 1102 14-15 0.26633787252484353 1102 16-17 0.2581411811590968 1102 18-19 0.16854339225294268 1102 20-21 0.2915201228975306 1102 22-23 0.3002448922547529 1102 24-25 0.19986235217305648 1102 26-27 0.13909867820860455 1102 28-29 0.2915297434566355 1102 30-31 0.22213334551901198 1102 32-33 0.28492604966111656 1102 34-35 0.3364612607081128 1102 36-37 0.2904835447885432 1102 38-39 0.33189572498879727 1102 40-41 0.3071607896894619 1102 42-43 0.2442016648122305 1102 44-45 0.3197120998296583 1102 46-47 0.3668742443501216 1102 48-49 0.34596352648633655 1102 50-51 0.44842093954077455 1102 52-53 0.4117683081388108 1102 54-55 0.34786682180842377 1102 56-57 0.21923764114067268 1102 58-59 0.10929592233974716 1102 60-61 0.06071336216755441 1102 62-63 0.2545830202680648 1102 64-65 0.2208470687947326 1102 66-67 0.24054917353539196 1102 68-69 0.26107506886856413 1102 70-71 0.39186901378268857 1102 72-73 0.5743928345548781 1102 74-75 0.5007485601682546 1102 76-77 0.3045682590400496 1102 78-79 0.3656422057283173 1102 80-81 0.6335596748097814 1102 82-83 0.6090101002433528 1102 84-85 0.5134833556026202 1102 86-87 0.5044721722754204 1102 88-89 0.5370834108478313 1102 90-91 0.6042124498544013 1102 92-93 0.6404892064618437 1102 94-95 0.6027633591214929 1102 96-97 0.5662127377937409 1102 98-99 0.7178759752127739 1102 100-101 1.4462574876266139 1103 1 0.02990300424540493 1103 2 0.10386281854321311 1103 3 0.15059197581798855 1103 4 0.034240342877097873 1103 5 0.04857871531982738 1103 6 0.07145642156983456 1103 7 0.090730770442633 1103 8 0.05837831924932857 1103 9 0.08875267307085721 1103 10-11 0.14877709157347851 1103 12-13 0.02098657434127915 1103 14-15 0.12279364607755383 1103 16-17 0.09553745218904197 1103 18-19 0.003966147125652242 1103 20-21 0.11159884091174632 1103 22-23 0.17346984087360795 1103 24-25 0.14253224973886347 1103 26-27 0.1588041066816004 1103 28-29 0.18369643394883894 1103 30-31 0.16827908633308652 1103 32-33 0.254868376236594 1103 34-35 0.16328660936165562 1103 36-37 0.011379674037002019 1103 38-39 0.11444922113257405 1103 40-41 0.2769046514888487 1103 42-43 0.12795842872615282 1103 44-45 0.26797958289204615 1103 46-47 0.4483707654310436 1103 48-49 0.2564283763971673 1103 50-51 0.18922506315546883 1103 52-53 0.3031247350580841 1103 54-55 0.46730805045624635 1103 56-57 0.3444151609256352 1103 58-59 0.32665688860982556 1103 60-61 0.25900003259943816 1103 62-63 0.3226277306471701 1103 64-65 0.2872892676497045 1103 66-67 0.25187089328613865 1103 68-69 0.2256773015167326 1103 70-71 0.17289938431276397 1103 72-73 0.17592770693983795 1103 74-75 0.23297534374410134 1103 76-77 0.25899327655767834 1103 78-79 0.35545356605715384 1103 80-81 0.32484471299704154 1103 82-83 0.3324509726798581 1103 84-85 0.2865952012693782 1103 86-87 0.07562552944991552 1103 88-89 0.3455395628750715 1103 90-91 0.4015936815019039 1103 92-93 0.24456413504381658 1103 94-95 0.21815268403074484 1103 96-97 0.23053307722562977 1103 98-99 0.3796486420872327 1103 100-101 1.097243777006124 1104 1 0.0027272058927607645 1104 2 -0.011269780198503554 1104 3 0.04918370123239413 1104 4 0.025355287909505364 1104 5 0.0037581393787959883 1104 6 -0.02597435175668039 1104 7 0.0037054631046729014 1104 8 0.006934432990021833 1104 9 -0.007045034182745269 1104 10-11 -0.026831245707100493 1104 12-13 -0.012948527128941123 1104 14-15 0.008269701548783814 1104 16-17 -0.01935528637171302 1104 18-19 -0.008624135382099496 1104 20-21 0.007843538056938826 1104 22-23 -0.08586165655692923 1104 24-25 -0.003933291645012105 1104 26-27 -0.07234317121506706 1104 28-29 -0.1751264734619582 1104 30-31 -0.09019741399594494 1104 32-33 -0.1133445170332621 1104 34-35 -0.10729353822510035 1104 36-37 -0.039754050678993735 1104 38-39 -0.026165460557841413 1104 40-41 -0.07347099980717786 1104 42-43 -0.12650541966262807 1104 44-45 -0.026006740611769885 1104 46-47 -0.02320803605750399 1104 48-49 0.01581821819605267 1104 50-51 0.003949424190999196 1104 52-53 0.027879775147141572 1104 54-55 0.13256566461240737 1104 56-57 0.028730782925585174 1104 58-59 -0.06540228439261142 1104 60-61 0.11477433871922216 1104 62-63 0.08366461235532796 1104 64-65 0.19258958700228135 1104 66-67 0.05310113831519914 1104 68-69 0.27972190600242897 1104 70-71 0.06844164407514342 1104 72-73 0.032020241972382735 1104 74-75 0.24865946243585313 1104 76-77 -0.060846084829357494 1104 78-79 0.08393630707270106 1104 80-81 0.19466315684757518 1104 82-83 0.3325404340446525 1104 84-85 0.16156480457377498 1104 86-87 0.057947906143056116 1104 88-89 0.1713376850219568 1104 90-91 0.24611804092395673 1104 92-93 0.2086516636973883 1104 94-95 0.12949142941649328 1104 96-97 0.19344637144374843 1104 98-99 0.20826731827800415 1104 100-101 0.7790305668039004 1105 1 -0.04528006128368389 1105 2 -0.022385137603407657 1105 3 -0.07278124661473839 1105 4 -0.01158145397670296 1105 5 0.015287100378138518 1105 6 0.030894638963076204 1105 7 -0.04863958453218231 1105 8 -0.03384310896354492 1105 9 -0.06912220446714201 1105 10-11 -0.04705367313648878 1105 12-13 -0.009619195881946041 1105 14-15 -0.11553869364740166 1105 16-17 -0.058379865305212775 1105 18-19 -0.08373624300541849 1105 20-21 0.05443750015520976 1105 22-23 -0.0678185072867663 1105 24-25 -0.042717854745120576 1105 26-27 0.021160628033278783 1105 28-29 0.005188155038368336 1105 30-31 -0.07886008295014335 1105 32-33 -0.06606440488736354 1105 34-35 -0.1174337971360373 1105 36-37 -0.08921330430082719 1105 38-39 0.002021774754993544 1105 40-41 -0.0730281459915858 1105 42-43 -0.03992449168158885 1105 44-45 -0.03626523055680053 1105 46-47 -0.004116799423762529 1105 48-49 -0.12396749523050943 1105 50-51 -0.24778166950483893 1105 52-53 -0.1872885421067707 1105 54-55 -0.14037785348390486 1105 56-57 -0.25276664797555526 1105 58-59 -0.2163028084909655 1105 60-61 -0.28095176658376175 1105 62-63 -0.278074102799998 1105 64-65 -0.2630670777289481 1105 66-67 -0.12617387718468365 1105 68-69 -0.24173723171198702 1105 70-71 -0.16115974375217235 1105 72-73 -0.20619574603447433 1105 74-75 -0.031548635118831214 1105 76-77 -0.1874562138255662 1105 78-79 0.005853115069871961 1105 80-81 -0.12155838706111055 1105 82-83 -0.013287801002469735 1105 84-85 -0.15304647365542223 1105 86-87 -0.22506739254270514 1105 88-89 -0.06119938621626986 1105 90-91 0.12359310169334137 1105 92-93 0.19584498743008538 1105 94-95 0.14166307518155818 1105 96-97 -0.14417102582237007 1105 98-99 0.024472186826965725 1105 100-101 0.6725582582889693 1106 1 -0.04753836262970168 1106 2 -0.15912961987420715 1106 3 -0.16504774097862907 1106 4 -0.11010599694299117 1106 5 -0.0382309666935754 1106 6 -0.03819558178839344 1106 7 -0.03446069333117663 1106 8 -0.00175920865345347 1106 9 -0.13246673765679873 1106 10-11 -0.041397741288434986 1106 12-13 -0.07427900057668069 1106 14-15 -0.04329109424922706 1106 16-17 -0.11940813267096217 1106 18-19 -0.14562637672424472 1106 20-21 -0.09582096721904776 1106 22-23 0.058123035438143233 1106 24-25 0.007105885654389965 1106 26-27 -0.019577296121951804 1106 28-29 -0.023906443782678366 1106 30-31 -0.06685793304302479 1106 32-33 -0.07021920877443932 1106 34-35 -0.16600168457734554 1106 36-37 -0.09983197364791607 1106 38-39 0.05465352067679419 1106 40-41 0.10992929288001818 1106 42-43 0.15970219593071988 1106 44-45 0.03705950350058629 1106 46-47 0.0839650826385494 1106 48-49 0.021598493196684387 1106 50-51 0.029791642671348484 1106 52-53 0.13017788013529952 1106 54-55 0.042718515071292984 1106 56-57 0.06521451469273387 1106 58-59 0.12138257539250219 1106 60-61 0.08429952694280729 1106 62-63 0.1708938915550391 1106 64-65 -0.011167402673713411 1106 66-67 -0.0020254659136895725 1106 68-69 0.061395044416237 1106 70-71 -0.0015984072882773148 1106 72-73 0.0832757501152841 1106 74-75 0.009469792165425872 1106 76-77 -0.12191583816287377 1106 78-79 0.0564003869852705 1106 80-81 0.1460793377280467 1106 82-83 0.17700356534386685 1106 84-85 0.09512662083341894 1106 86-87 0.18323604204758226 1106 88-89 0.27298625186489645 1106 90-91 0.492463705150481 1106 92-93 0.3770792884419336 1106 94-95 0.5237048339282424 1106 96-97 0.5000919184981605 1106 98-99 0.4947370045171162 1106 100-101 0.8636163155628971 1107 1 -0.15281480890621424 1107 2 -0.08968579597941329 1107 3 -0.10570004690734436 1107 4 -0.10741173927297609 1107 5 -0.1405285543402286 1107 6 -0.049207775082280136 1107 7 -0.09618896273262578 1107 8 -0.03567193120202461 1107 9 -0.10423559144592787 1107 10-11 -0.034617681914831167 1107 12-13 -0.06137486523088853 1107 14-15 0.01028427635991136 1107 16-17 0.013127084219291874 1107 18-19 -0.0605102005767435 1107 20-21 -0.07705481879138887 1107 22-23 -0.03160782696489406 1107 24-25 -0.02058910565953198 1107 26-27 -0.12258479991112381 1107 28-29 -4.811939009172761E-4 1107 30-31 -0.06935020731005181 1107 32-33 -0.04521583136871499 1107 34-35 -0.2244198980870209 1107 36-37 -0.10548982222328362 1107 38-39 -0.15671999554492544 1107 40-41 -0.19939976267043846 1107 42-43 -0.23524270675108738 1107 44-45 -0.182205245187383 1107 46-47 -0.4432170188824358 1107 48-49 -0.3904268953768124 1107 50-51 -0.26917888969503423 1107 52-53 -0.13516710831013512 1107 54-55 -0.26455488347659895 1107 56-57 -0.23045501666612012 1107 58-59 -0.2773854712561459 1107 60-61 -0.3445947804828009 1107 62-63 -0.3133703720026304 1107 64-65 -0.3832206987969826 1107 66-67 -0.15068009518029157 1107 68-69 -0.33647022366517376 1107 70-71 -0.3767778856687798 1107 72-73 -0.25356224201106414 1107 74-75 -0.3523240557479461 1107 76-77 -0.5916259285292966 1107 78-79 -0.10570504147731086 1107 80-81 -0.022619103959428344 1107 82-83 -0.18117368249478716 1107 84-85 -0.2344290632677115 1107 86-87 -0.25103666832909965 1107 88-89 -0.16032155154180217 1107 90-91 0.04495374543876807 1107 92-93 -0.0034966365640620722 1107 94-95 -0.019273281804547793 1107 96-97 -0.007923769055725671 1107 98-99 -0.18639101726959595 1107 100-101 0.4768742787717777 1108 1 0.021904309689361412 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-3.3467887858445344 2316 100-101 -3.9110303907127353 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 19.0 5 75.0 6 181.0 7 425.0 8 950.0 9 1683.0 10 3039.0 11 4653.0 12 7014.0 13 9877.0 14 11649.0 15 15519.0 16 20479.0 17 29616.0 18 28991.0 19 17887.0 20 15397.0 21 15900.0 22 17079.0 23 18806.0 24 21658.0 25 25782.0 26 30476.0 27 37058.0 28 45060.0 29 54453.0 30 66297.0 31 80640.0 32 99856.0 33 126554.0 34 169466.0 35 243363.0 36 378172.0 37 634042.0 38 543095.0 39 43997.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94721136468709 20.901390310638295 15.219723505061665 21.931674819612947 2 15.084493438417033 22.14383943320352 40.08494569229072 22.686721436088725 3 18.18911873632819 26.66790341843628 32.083067153847544 23.059910691387987 4 12.268140080228065 18.25296634202524 38.49386335832021 30.985030219426484 5 12.282704313280362 38.9477866061294 35.156001702610666 13.61350737797957 6 29.192437747697674 38.24893684868791 18.110976361133407 14.447649042481004 7 26.078164367147856 35.00207327364055 22.001140034470613 16.918622324740983 8 25.16312176483295 35.57638248688553 20.786687632328597 18.473808115952927 9 26.056207884966476 17.04954538731199 21.852942647469348 35.04130408025218 10-11 23.872531092897205 27.43728480253177 28.24282876107623 20.447355343494795 12-13 25.19142753669198 25.487733633464376 28.62752832084699 20.69331050899666 14-15 22.27157276004022 26.29941849998998 27.79831471943294 23.630694020536865 16-17 20.485858868198665 29.682117175661332 28.612291923245003 21.219732032894996 18-19 20.318489592662917 28.083262953171683 31.436840706963253 20.161406747202147 20-21 21.251161228328616 27.25069017488214 31.23314910934364 20.26499948744561 22-23 21.117045785472754 27.117248678975304 30.95448978136806 20.81121575418388 24-25 20.993766700139364 27.305067052400794 31.24244248306176 20.45872376439808 26-27 20.827887073449222 27.331209099844532 31.368115853608792 20.472787973097457 28-29 20.462927065106467 27.385160326658536 31.630635925699828 20.52127668253516 30-31 20.6796542155559 27.495350652824037 31.311876754257305 20.51311837736276 32-33 20.54000531353963 27.68996170207785 30.96883775720387 20.80119522717865 34-35 21.037434740875426 27.38591757929529 30.991779070683105 20.584868609146174 36-37 21.278721136968944 27.09331026135472 30.915688661669154 20.712279940007182 38-39 20.959051867492605 27.49580369133261 31.05396125684744 20.491183184327348 40-41 20.766113477574798 27.405872396833285 31.23787639379903 20.590137731792886 42-43 20.654117310708383 27.643220735328622 30.634524793216205 21.06813716074679 44-45 20.759066536440574 27.877558860236746 30.300196917600193 21.06317768572249 46-47 20.965055580375395 27.900874909615492 29.76546051715634 21.36860899285277 48-49 21.158039234465033 27.946885735517636 29.65059298632061 21.244482043696717 50-51 21.15863134076831 28.25022515276513 29.442692369493866 21.14845113697269 52-53 21.14955485804017 28.4130003657049 29.440503431276742 20.99694134497819 54-55 20.592874678145993 28.225767261998296 29.6158005798072 21.56555748004851 56-57 20.948222074742134 28.788051842836992 28.990909874493344 21.27281620792753 58-59 21.027678695576917 28.09200314414545 29.331269633173573 21.549048527104066 60-61 21.10806547014656 28.175787183891583 29.492479552772604 21.223667793189254 62-63 21.15789051767284 28.05415908624144 29.609061110316958 21.178889285768758 64-65 20.827758473668453 27.949485031945187 29.652057814900946 21.570698679485414 66-67 20.64760985206088 28.39353404632185 29.24213592918332 21.716720172433945 68-69 20.609322047726174 28.231469874423855 29.463678265132316 21.695529812717663 70-71 20.65168541717139 28.24557775521041 29.225461148199873 21.877275679418336 72-73 20.820615540578167 28.02631443597912 28.930893125138113 22.222176898304596 74-75 20.628558395552236 28.418427412189146 28.81085962642766 22.14215456583096 76-77 20.699821214336094 27.93545532098668 29.256689900989535 22.108033563687687 78-79 20.485515187491256 28.05404986465187 29.20098403401093 22.259450913845946 80-81 20.414314207546575 28.422169591300833 28.951430771637334 22.21208542951526 82-83 20.459557330361346 28.276632521203616 28.88385872227462 22.37995142616042 84-85 20.2866367315985 27.866500331830196 28.860447933519033 22.986415003052272 86-87 20.302181386984998 27.939202798723123 29.095153886432602 22.663461927859274 88-89 20.533830239988458 27.541282949088743 29.055429022899688 22.869457788023112 90-91 20.06703813183039 27.671639506761654 29.34736145245133 22.91396090895663 92-93 20.10779249386265 27.80437306439776 29.086060542427976 23.001773899311615 94-95 19.924674013634302 27.497177403764812 29.048408190512742 23.529740392088147 96-97 20.0249929102215 27.255201343501938 28.840172538339342 23.879633207937218 98-99 19.69902593383331 27.296199329387317 28.863374282113487 24.141400454665895 100-101 19.78641881463914 26.94048224165005 29.577604923934338 23.69549401977647 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2414.0 1 1705.0 2 1097.5 3 1258.0 4 1235.5 5 1295.0 6 2033.5 7 3903.5 8 6024.5 9 7053.0 10 6311.5 11 5318.5 12 5299.0 13 5548.5 14 5920.0 15 6112.5 16 6256.5 17 6550.0 18 6819.5 19 7194.0 20 7964.0 21 9213.5 22 10819.5 23 12613.0 24 14738.5 25 17742.0 26 22431.5 27 27242.5 28 30179.5 29 33993.5 30 40248.5 31 46406.0 32 53526.0 33 61867.5 34 69578.0 35 75010.0 36 82615.0 37 91688.0 38 93571.5 39 91469.0 40 89604.0 41 90496.5 42 92110.5 43 95060.0 44 98587.5 45 99177.5 46 97812.0 47 99449.5 48 121228.5 49 122968.5 50 100420.5 51 90589.5 52 83013.0 53 78758.0 54 74853.5 55 67193.5 56 58791.0 57 51480.5 58 45829.5 59 40580.5 60 34213.0 61 27004.5 62 21182.5 63 16141.5 64 11652.5 65 8529.5 66 6035.5 67 4691.0 68 3312.0 69 2062.0 70 1454.0 71 1064.5 72 791.0 73 589.0 74 499.0 75 436.5 76 261.0 77 103.0 78 51.0 79 30.5 80 18.0 81 9.0 82 5.5 83 4.5 84 1.5 85 1.0 86 1.0 87 1.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.412845712615542 2 0.0 3 0.0 4 0.0 5 4.6112159670092326E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013656293440758114 16-17 0.002163724415288948 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0023765497676124505 36-37 0.00741341643926869 38-39 0.013000081937760644 40-41 0.0026425814580168295 42-43 8.690368553209708E-4 44-45 4.7885704272788185E-4 46-47 2.660316904043788E-4 48-49 2.837671364313374E-4 50-51 5.675342728626748E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.7735446026958588E-4 60-61 0.002323343429531575 62-63 0.0 64-65 3.192380284852546E-4 66-67 0.0016493964805071486 68-69 0.0016671319265341073 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12595713768345987 78-79 5.3206338080875766E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14950981000726088 88-89 0.4439359495008004 90-91 0.3411590397745754 92-93 0.0 94-95 0.0 96-97 4.7885704272788185E-4 98-99 0.0 100-101 1.418835682156687E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2819213.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.221555786848974 #Duplication Level Percentage of deduplicated Percentage of total 1 66.52326994230445 24.76099603281099 2 15.467213967367085 11.514275351069674 3 6.542081537502682 7.305193587308125 4 3.401866262207845 5.064910194326749 5 1.9541899584867923 3.6368995278958107 6 1.2598815539228612 2.813685092649705 7 0.8892077516394703 2.3168387153622962 8 0.6292310580933166 1.8736767145310715 9 0.471863166553236 1.5807133059858438 >10 2.475951370521744 16.679731926934654 >50 0.2215244229668028 5.727041039866231 >100 0.15073616799047715 10.49641573624833 >500 0.009641668270414627 2.42633435160317 >1k 0.002863861862497099 1.8844287325597633 >5k 2.863861862497099E-4 0.8053077798154858 >10k+ 1.9092412416647328E-4 1.1135519110323644 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16666 0.5911578869705837 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14673 0.5204643991071267 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9994 0.3544960951868482 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6918 0.245387631228999 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5752 0.2040285710941316 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3757 0.13326414144656681 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3160 0.11208801889037827 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3086 0.10946317287838839 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2969 0.1053130785080801 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.0641267616175152E-4 0.0 0.0 0.0 0.0 3 1.0641267616175152E-4 0.0 0.0 0.0 0.0 4 1.0641267616175152E-4 0.0 0.0 0.0 0.0 5 1.0641267616175152E-4 0.0 0.0 0.0 0.0 6 1.0641267616175152E-4 0.0 0.0 0.0 0.0 7 1.0641267616175152E-4 0.0 0.0 0.0 0.0 8 1.0641267616175152E-4 0.0 0.0 0.0 0.0 9 1.0641267616175152E-4 0.0 0.0 0.0 0.0 10-11 1.0641267616175152E-4 0.0 0.0 0.0 0.0 12-13 1.0641267616175152E-4 0.0 0.0 0.0 0.0 14-15 1.418835682156687E-4 0.0 0.0 0.0 0.0 16-17 1.418835682156687E-4 0.0 0.0 0.0 0.0 18-19 1.9508990629654445E-4 0.0 0.0 0.0 0.0 20-21 2.1282535232350304E-4 0.0 0.0 3.5470892053917175E-5 0.0 22-23 2.482962443774202E-4 0.0 0.0 1.9508990629654445E-4 0.0 24-25 3.192380284852546E-4 0.0 0.0 4.6112159670092326E-4 0.0 26-27 3.192380284852546E-4 0.0 0.0 0.001294687559967977 0.0 28-29 3.369734745122132E-4 0.0 0.0 0.005941374419031127 0.0 30-31 3.5470892053917176E-4 0.0 0.0 0.018409392975983013 0.0 32-33 3.7244436656613036E-4 0.0 0.0 0.04100435121432826 0.0 34-35 4.7885704272788185E-4 0.0 0.0 0.07670580406659588 0.0 36-37 6.03005164916592E-4 0.0 0.0 0.1343460036542113 0.0 38-39 6.562115029974678E-4 0.0 0.0 0.21736562650640445 0.0 40-41 6.739469490244263E-4 0.0 0.0 0.309199766033996 0.0 42-43 6.916823950513849E-4 0.0 0.0 0.4052726771620307 0.0 44-45 8.513014092940121E-4 0.0 0.0 0.5258027683612412 0.0 46-47 9.399786394288051E-4 0.0 0.0 0.6688391405686622 0.0 48-49 9.931849775096809E-4 0.0 0.0 0.830001138615635 0.0 50-51 9.931849775096809E-4 0.0 0.0 0.9985765531018763 0.0 52-53 9.931849775096809E-4 0.0 0.0 1.1659991635963654 0.0 54-55 9.931849775096809E-4 0.0 0.0 1.351565135376433 0.0 56-57 9.931849775096809E-4 0.0 0.0 1.5704028039030753 0.0 58-59 9.931849775096809E-4 0.0 0.0 1.8244985391313109 0.0 60-61 0.0010109204235366396 0.0 0.0 2.1132493358962234 0.0 62-63 0.001028655869563598 0.0 0.0 2.3903124737293706 0.0 64-65 0.001028655869563598 0.0 0.0 2.678885916034014 0.0 66-67 0.001028655869563598 0.0 0.0 3.0095633072066565 0.0 68-69 0.001028655869563598 0.0 0.0 3.3839408373897255 0.0 70-71 0.0010641267616175152 0.0 0.0 3.8040403474302935 0.0 72-73 0.0010641267616175152 0.0 0.0 4.2376187964513505 0.0 74-75 0.0010641267616175152 0.0 0.0 4.692249219906406 0.0 76-77 0.0010641267616175152 0.0 0.0 5.179317774144771 0.0 78-79 0.0010641267616175152 0.0 0.0 5.714839566928784 0.0 80-81 0.001206010329833184 0.0 0.0 6.30067682009128 0.0 82-83 0.0012414812218871011 0.0 0.0 6.897882494157058 0.0 84-85 0.0012592166679140596 0.0 0.0 7.525096542900448 0.0 86-87 0.001294687559967977 0.0 0.0 8.186043409987114 0.0 88-89 0.0013124230059949355 0.0 0.0 8.882514375465778 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12610 0.0 24.678692 1 GGTATCA 19890 0.0 24.654976 1 TACATGG 13090 0.0 23.309616 2 ACATGGG 12720 0.0 22.756554 3 GAGTACT 13555 0.0 22.598307 12-13 TACCGTA 105 0.0022096804 22.596672 2 GGACCGA 365 0.0 22.101402 6 GTATCAA 25700 0.0 21.306442 1 CGTATAG 315 1.2732926E-11 21.180758 1 CATGGGG 4820 0.0 20.871447 4 GTACTTT 14880 0.0 20.617916 14-15 AGTACTT 14200 0.0 20.402178 12-13 CATGGGA 7205 0.0 19.231476 4 ATCAACG 30185 0.0 17.98451 3 TCAACGC 30215 0.0 17.966652 4 CAACGCA 30460 0.0 17.837719 5 TATCAAC 30545 0.0 17.803617 2 AACGCAG 30750 0.0 17.684927 6 ACTTTTT 17620 0.0 17.518988 16-17 AGTACAT 10015 0.0 17.152262 2 >>END_MODULE