FastQCFastQC Report
Fri 27 May 2016
SRR938999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090930
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111930.3621240209257408No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT110710.3581769888027228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90010.2912068536006962No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT59630.19291928319308427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57420.18576933156040415No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46720.1511519186781972No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41180.13322851051301712No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40680.13161087439702612No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34910.11294335361849023No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32000.10352871142342274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG110700.024.2678011
TACATGG111950.023.396692
ACATGGG111250.022.5221063
GTATAGG12500.022.023161
GAGTACT96750.021.43318212-13
GTACTTT102600.019.93521714-15
AGTACTT104600.019.8699312-13
CATGGGA61000.018.5575834
CATGGGG53450.018.4318524
TACCGTA1300.007710259418.2094637
GTATACG1350.00944536317.5791511
TTTGCGT6250.017.4301533
GTACCGT2756.120754E-717.216226
ACCGTAT1700.00182811216.7098628
ATGGGAT28150.016.4892565
AGTACAT82350.016.3921012
ACTTTTT127300.016.38505216-17
GTATCAA293300.016.2150141
AGAGTAC199150.016.05876210-11
TACTTTT128500.015.82503314-15
Produced by FastQC (version 0.11.5)