##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938999_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3090930 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.793861394467037 33.0 31.0 34.0 30.0 34.0 2 31.87280236045462 33.0 31.0 34.0 30.0 34.0 3 31.919922482877322 34.0 31.0 34.0 30.0 34.0 4 35.45113541878981 37.0 35.0 37.0 33.0 37.0 5 35.493323045167635 37.0 35.0 37.0 33.0 37.0 6 35.57908946498303 37.0 35.0 37.0 33.0 37.0 7 35.502775540047814 37.0 35.0 37.0 33.0 37.0 8 35.49176234984293 37.0 35.0 37.0 33.0 37.0 9 37.09146988123316 39.0 37.0 39.0 33.0 39.0 10-11 36.98100102558129 39.0 37.0 39.0 33.0 39.0 12-13 36.874919199076004 39.0 37.0 39.0 33.0 39.0 14-15 38.07696405289023 40.0 38.0 41.0 33.0 41.0 16-17 37.9942137156131 40.0 38.0 41.0 33.0 41.0 18-19 37.925899486562294 40.0 38.0 41.0 33.0 41.0 20-21 38.03617131413523 40.0 38.0 41.0 33.0 41.0 22-23 37.97051017007826 40.0 38.0 41.0 33.0 41.0 24-25 37.886940823635605 40.0 38.0 41.0 32.5 41.0 26-27 37.7631402199338 40.0 38.0 41.0 32.0 41.0 28-29 37.63940513049471 40.0 37.0 41.0 32.0 41.0 30-31 37.44969863439159 40.0 37.0 41.0 31.5 41.0 32-33 37.25199826589409 40.0 37.0 41.0 31.0 41.0 34-35 36.93268094068775 40.0 36.0 41.0 30.5 41.0 36-37 36.66606830306736 39.5 36.0 41.0 30.0 41.0 38-39 36.43034070651875 39.0 35.5 41.0 30.0 41.0 40-41 36.2865377087155 39.0 35.0 41.0 30.0 41.0 42-43 36.076928141368455 39.0 35.0 41.0 29.0 41.0 44-45 36.01723494223421 39.0 35.0 41.0 28.5 41.0 46-47 35.803917591145705 39.0 35.0 41.0 27.5 41.0 48-49 35.558964130536765 39.0 35.0 41.0 26.5 41.0 50-51 34.44662933162511 38.0 34.0 40.0 25.0 40.5 52-53 34.5485747008182 38.0 34.0 39.5 25.0 40.5 54-55 35.04877496416936 39.0 35.0 40.0 26.0 41.0 56-57 34.95362851957178 39.0 35.0 40.5 25.5 41.0 58-59 34.742172582361945 38.0 34.0 40.0 25.0 41.0 60-61 34.41999527650254 38.0 34.0 40.0 24.0 41.0 62-63 34.03755487830524 37.5 34.0 40.0 23.0 41.0 64-65 33.65078649467959 37.0 33.0 40.0 22.0 41.0 66-67 33.232994923857866 36.0 33.0 39.0 21.5 41.0 68-69 32.78409556347119 36.0 32.5 39.0 20.0 40.5 70-71 32.33619299045918 35.0 32.0 38.5 18.5 40.0 72-73 31.897045549397752 35.0 31.5 37.5 17.5 40.0 74-75 31.43040444138172 35.0 31.0 37.0 14.0 39.0 76-77 31.005808446001687 35.0 31.0 36.5 9.5 39.0 78-79 30.615626850171306 35.0 31.0 36.0 7.0 39.0 80-81 30.170242451301064 34.0 30.0 36.0 4.5 37.5 82-83 29.70890395447325 34.0 30.0 35.0 2.0 37.0 84-85 29.297563354718484 34.0 29.0 35.0 2.0 36.5 86-87 28.871953101493723 34.0 29.0 35.0 2.0 36.0 88-89 28.394662447871674 34.0 29.0 35.0 2.0 36.0 90-91 27.936690251801238 34.0 27.5 35.0 2.0 35.0 92-93 27.441863128572955 33.5 26.5 35.0 2.0 35.0 94-95 26.91023138667003 33.0 25.5 35.0 2.0 35.0 96-97 26.259100982552177 33.0 24.5 35.0 2.0 35.0 98-99 25.406178075854193 32.5 21.0 35.0 2.0 35.0 100-101 22.201082036797985 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2275365716135127 1101 2 0.33818053197166265 1101 3 0.3952492879617573 1101 4 0.24895981961613955 1101 5 0.18913433669289503 1101 6 0.28101790935338045 1101 7 0.3465853175046192 1101 8 0.32055664797653094 1101 9 0.40234670897548597 1101 10-11 0.3613825073510242 1101 12-13 0.33150600649598516 1101 14-15 0.47492486646161325 1101 16-17 0.5047267051633142 1101 18-19 0.597853215044239 1101 20-21 0.47064374810894805 1101 22-23 0.5433253752480454 1101 24-25 0.5642245434353015 1101 26-27 0.5380131356625455 1101 28-29 0.5319121754471965 1101 30-31 0.5008517630227445 1101 32-33 0.5009171941440584 1101 34-35 0.46984607561363134 1101 36-37 0.38015344703167386 1101 38-39 0.5644309723981422 1101 40-41 0.366045937087371 1101 42-43 0.32206184736323706 1101 44-45 0.2958213853488658 1101 46-47 0.35137180485433817 1101 48-49 0.25825365825261315 1101 50-51 0.30416163369283566 1101 52-53 0.17214010161008986 1101 54-55 0.2981879013943569 1101 56-57 0.24058409314955043 1101 58-59 0.1451461143251862 1101 60-61 0.31795985296266593 1101 62-63 0.24304202932896857 1101 64-65 0.11896255390383459 1101 66-67 0.13133335089401044 1101 68-69 0.15634135213621647 1101 70-71 0.10712645304583646 1101 72-73 0.04558328921465815 1101 74-75 0.03267099085004688 1101 76-77 -0.07403545713018289 1101 78-79 -0.23662120469503023 1101 80-81 -0.26962522099671915 1101 82-83 -0.16507965694785653 1101 84-85 -0.1287339464576469 1101 86-87 -0.1667943239687375 1101 88-89 -0.27082098948579514 1101 90-91 -0.23556542788022128 1101 92-93 -0.12335324895043343 1101 94-95 -0.3750609179250546 1101 96-97 -0.4601965429425299 1101 98-99 -0.7882522402392773 1101 100-101 -0.45155093890743814 1102 1 0.3035286035798066 1102 2 0.2908578266064801 1102 3 0.38790945779739516 1102 4 0.25058991218421056 1102 5 0.3342140464540293 1102 6 0.3263265382513296 1102 7 0.32290042557916365 1102 8 0.34489328268868036 1102 9 0.4621216738137477 1102 10-11 0.4548803799919483 1102 12-13 0.4937384682888464 1102 14-15 0.6074066676844794 1102 16-17 0.662855247083769 1102 18-19 0.610061990473973 1102 20-21 0.545408061439808 1102 22-23 0.6204774505265434 1102 24-25 0.6793961739398 1102 26-27 0.6185247022580072 1102 28-29 0.49980611938848796 1102 30-31 0.5645217369530329 1102 32-33 0.5653499866632856 1102 34-35 0.5669991513099504 1102 36-37 0.42543118067337105 1102 38-39 0.527468476296832 1102 40-41 0.49660929001712617 1102 42-43 0.39444060555047855 1102 44-45 0.5929226747108913 1102 46-47 0.6792160809931929 1102 48-49 0.6469534975972806 1102 50-51 0.6041273546704247 1102 52-53 0.6525348547128615 1102 54-55 0.7273156547501642 1102 56-57 0.734299850907199 1102 58-59 0.6841226868937298 1102 60-61 0.788248179263654 1102 62-63 0.6931278004451329 1102 64-65 0.44658026483016755 1102 66-67 0.6200617315706438 1102 68-69 0.7041002690956475 1102 70-71 0.5973319212119534 1102 72-73 0.5282804745096925 1102 74-75 0.6034123004558261 1102 76-77 0.6005065574759172 1102 78-79 0.5366311536428618 1102 80-81 0.6111691107536004 1102 82-83 0.5459930557733408 1102 84-85 0.4242264075725686 1102 86-87 0.352325426044505 1102 88-89 0.16570397093885347 1102 90-91 0.22607737587899734 1102 92-93 0.31188882273832874 1102 94-95 0.14420006754534853 1102 96-97 0.16466369280298565 1102 98-99 0.016451488965270045 1102 100-101 0.10664101184846686 1103 1 0.21938557337196585 1103 2 0.2527209947427487 1103 3 0.3173662082769546 1103 4 0.21287301740626674 1103 5 0.06773942374296382 1103 6 0.2573994102428756 1103 7 0.2531628735324176 1103 8 0.13502363406876583 1103 9 0.3803524697817693 1103 10-11 0.35628478776795447 1103 12-13 0.24292022723987117 1103 14-15 0.36897838857575493 1103 16-17 0.3520129078269605 1103 18-19 0.4499414339900767 1103 20-21 0.49602455089666364 1103 22-23 0.372943626503087 1103 24-25 0.3325350491337744 1103 26-27 0.38624822876966647 1103 28-29 0.34422179238584505 1103 30-31 0.2989557053912151 1103 32-33 0.33567239654224323 1103 34-35 0.32909552087204474 1103 36-37 0.3304672261821864 1103 38-39 0.4617592298275781 1103 40-41 0.36057203450776143 1103 42-43 0.34193519908773595 1103 44-45 0.4357002294917791 1103 46-47 0.37725950139712694 1103 48-49 0.38999690345792715 1103 50-51 0.26231329190455455 1103 52-53 0.20079380646263445 1103 54-55 0.4008174792041004 1103 56-57 0.3373535025055858 1103 58-59 0.25809505903288965 1103 60-61 0.44318387617015276 1103 62-63 0.3356648094459658 1103 64-65 0.29686851255450364 1103 66-67 0.17234408054235928 1103 68-69 0.22538889785381855 1103 70-71 0.3037071842634518 1103 72-73 0.16133454059142593 1103 74-75 0.07658595589362704 1103 76-77 0.16694696713090806 1103 78-79 0.1932782305171621 1103 80-81 0.18885147252339962 1103 82-83 0.2928809765648417 1103 84-85 0.2589873452066378 1103 86-87 0.3455531679246313 1103 88-89 0.2781662129593201 1103 90-91 0.34898041731424456 1103 92-93 0.25499298313321006 1103 94-95 0.19639570223571567 1103 96-97 0.10078671728077282 1103 98-99 0.06623130377120034 1103 100-101 0.08658947047939591 1104 1 0.30015305294823236 1104 2 0.32254419805599 1104 3 0.29294357271277605 1104 4 0.274235530987724 1104 5 0.23129340156321376 1104 6 0.30376698609914854 1104 7 0.28813338778042663 1104 8 0.35633551648596296 1104 9 0.3277679323171441 1104 10-11 0.42543386271402994 1104 12-13 0.3997535407954089 1104 14-15 0.41612192742145027 1104 16-17 0.46964073342211776 1104 18-19 0.43427363031038624 1104 20-21 0.4047125590703686 1104 22-23 0.43076759736323567 1104 24-25 0.5142803935662386 1104 26-27 0.4611025673874636 1104 28-29 0.5115217694099385 1104 30-31 0.5541919827550643 1104 32-33 0.6350876469922966 1104 34-35 0.5970417690236545 1104 36-37 0.7183763425690657 1104 38-39 0.7427516713421056 1104 40-41 0.6701278251526119 1104 42-43 0.7660599894220326 1104 44-45 0.7735189150508646 1104 46-47 0.8118395934859208 1104 48-49 0.7018528099922605 1104 50-51 0.5076909679038195 1104 52-53 0.541321629617137 1104 54-55 0.6573586704671968 1104 56-57 0.7129755563500098 1104 58-59 0.6269846660943159 1104 60-61 0.606563641041582 1104 62-63 0.6166189577760903 1104 64-65 0.6284828606319763 1104 66-67 0.6575372423053949 1104 68-69 0.693845834011924 1104 70-71 0.54635215763075 1104 72-73 0.5567335513648572 1104 74-75 0.6036764902392484 1104 76-77 0.6501598242148532 1104 78-79 0.5448748879850491 1104 80-81 0.6183684481866081 1104 82-83 0.7924791085307312 1104 84-85 0.6744460933477363 1104 86-87 0.6745850404781777 1104 88-89 0.6679807308733778 1104 90-91 0.3869643106117593 1104 92-93 0.39667860703017865 1104 94-95 0.4381712940758149 1104 96-97 0.3293393779838034 1104 98-99 0.22418353575888617 1104 100-101 0.5733833715112908 1105 1 0.1286318814089924 1105 2 0.19069299840818488 1105 3 0.157499601411768 1105 4 0.23366994753427406 1105 5 0.23076164319834191 1105 6 0.20597120196206475 1105 7 0.21812901825670394 1105 8 0.2525294078824132 1105 9 0.09147490764458865 1105 10-11 0.2873181341651758 1105 12-13 0.2328713508520721 1105 14-15 0.3297927403640628 1105 16-17 0.3392658907085533 1105 18-19 0.39214222263382226 1105 20-21 0.3187908148466079 1105 22-23 0.21027901327195764 1105 24-25 0.3341882557521245 1105 26-27 0.3207238151000311 1105 28-29 0.2839426826732421 1105 30-31 0.3897449457474025 1105 32-33 0.45402803044968465 1105 34-35 0.3570300731556486 1105 36-37 0.3500352902131638 1105 38-39 0.3346582785219212 1105 40-41 0.4713064584162865 1105 42-43 0.4366671866048861 1105 44-45 0.5516020810265942 1105 46-47 0.4310694520231735 1105 48-49 0.38215515193494554 1105 50-51 0.3753310073844105 1105 52-53 0.38584860168737833 1105 54-55 0.5525523469074827 1105 56-57 0.49111533605279334 1105 58-59 0.4133734471661583 1105 60-61 0.42495102865307643 1105 62-63 0.3888468563637204 1105 64-65 0.38506372239967845 1105 66-67 0.40691554731505164 1105 68-69 0.36870492743196337 1105 70-71 0.2717375651111311 1105 72-73 0.22523976326939277 1105 74-75 0.06421994916549068 1105 76-77 0.1741129454674919 1105 78-79 0.1776642914910873 1105 80-81 0.30274427979238894 1105 82-83 0.40503963535167387 1105 84-85 0.3771540951143173 1105 86-87 0.40080639510562577 1105 88-89 0.4235363855111025 1105 90-91 0.3717830903647652 1105 92-93 0.3938409392951421 1105 94-95 0.38049387428192105 1105 96-97 0.29424590725374244 1105 98-99 0.2336918845529503 1105 100-101 0.48542652513407347 1106 1 0.1382963462973379 1106 2 0.17570798342316962 1106 3 0.17136136154218562 1106 4 0.21173789948282717 1106 5 0.10054360253253947 1106 6 0.21259020484972524 1106 7 0.2401418313142969 1106 8 0.2213752386685428 1106 9 0.34998381167588377 1106 10-11 0.25611488467833965 1106 12-13 0.20267580013413777 1106 14-15 0.28810184419555895 1106 16-17 0.29575529682482227 1106 18-19 0.373797383393466 1106 20-21 0.354550453218188 1106 22-23 0.2732173187475837 1106 24-25 0.4132317247209585 1106 26-27 0.33950664918137363 1106 28-29 0.265959422152676 1106 30-31 0.18739816317673785 1106 32-33 0.3311720307280055 1106 34-35 0.3881009922862759 1106 36-37 0.35794218431408353 1106 38-39 0.39437406025263044 1106 40-41 0.4302796991059452 1106 42-43 0.3546900841053926 1106 44-45 0.4039667561524638 1106 46-47 0.23801188769694903 1106 48-49 0.35375941831533453 1106 50-51 0.24915370210263177 1106 52-53 0.3114143294919174 1106 54-55 0.37182822028783846 1106 56-57 0.33862230669409144 1106 58-59 0.33473131815089374 1106 60-61 0.38923053024600307 1106 62-63 0.508374095293938 1106 64-65 0.28314014846117885 1106 66-67 0.38487850475561913 1106 68-69 0.3685061212630103 1106 70-71 0.2953420700589149 1106 72-73 0.2024224064968152 1106 74-75 0.23788086536372077 1106 76-77 0.10341560797910887 1106 78-79 0.2859167355644274 1106 80-81 0.3523886652576742 1106 82-83 0.2795755472010164 1106 84-85 0.4031169382413431 1106 86-87 0.4837750433727841 1106 88-89 0.5479244352722894 1106 90-91 0.5019284425250454 1106 92-93 0.6795744843570475 1106 94-95 0.4963487905099697 1106 96-97 0.6011902067353567 1106 98-99 0.6132706391317022 1106 100-101 0.7835227075586175 1107 1 0.07313663039135321 1107 2 0.20061309489544144 1107 3 0.15599198796385494 1107 4 0.17551375878107223 1107 5 0.17935202462454214 1107 6 0.1934504722771564 1107 7 0.026149521799595732 1107 8 0.10399179292689098 1107 9 0.1341780585566994 1107 10-11 0.1860781221042629 1107 12-13 0.25895683253457236 1107 14-15 0.32217175438486834 1107 16-17 0.24551117941651768 1107 18-19 0.193679988350695 1107 20-21 0.25052230889153293 1107 22-23 0.20178361149298496 1107 24-25 0.22333720796076761 1107 26-27 0.23033108179685513 1107 28-29 0.3432898860503002 1107 30-31 0.40939291800732036 1107 32-33 0.362327442480435 1107 34-35 0.319626183512959 1107 36-37 0.3034462656390602 1107 38-39 0.4328604494852897 1107 40-41 0.32529032695239124 1107 42-43 0.48467336782002235 1107 44-45 0.2921908040780039 1107 46-47 0.5373448930001032 1107 48-49 0.5862633985805061 1107 50-51 0.5554757382754119 1107 52-53 0.6030278569263388 1107 54-55 0.6588880924223304 1107 56-57 0.6159231153381839 1107 58-59 0.5306243140438909 1107 60-61 0.6180160067926792 1107 62-63 0.5788513792040604 1107 64-65 0.6023221273446424 1107 66-67 0.5824055216856152 1107 68-69 0.5526488442117028 1107 70-71 0.543251209012837 1107 72-73 0.6147957711089873 1107 74-75 0.6635792134501308 1107 76-77 0.6734533645840308 1107 78-79 0.7111483861692101 1107 80-81 0.6759690649574743 1107 82-83 0.7034867935003248 1107 84-85 0.771462854348453 1107 86-87 0.8597653880198415 1107 88-89 0.8541107862347594 1107 90-91 0.9039668684440656 1107 92-93 0.7576845997805925 1107 94-95 0.8793217048261681 1107 96-97 0.9158047795588864 1107 98-99 0.89871902159501 1107 100-101 1.0873788845491212 1108 1 0.31806948643633604 1108 2 0.24375409720098773 1108 3 0.2989661415859892 1108 4 0.23203501429213702 1108 5 0.31139000545211815 1108 6 0.2899112577805312 1108 7 0.3177312473183491 1108 8 0.30398774811032325 1108 9 0.3374604272012789 1108 10-11 0.4398660928197913 1108 12-13 0.4284816677082901 1108 14-15 0.5719133917266177 1108 16-17 0.5608096868792174 1108 18-19 0.553583721716393 1108 20-21 0.4971201125561393 1108 22-23 0.5688926799717251 1108 24-25 0.616191028290018 1108 26-27 0.5910559129257607 1108 28-29 0.5514376634235987 1108 30-31 0.5519949485052322 1108 32-33 0.624416387996753 1108 34-35 0.6484055589932751 1108 36-37 0.6057138909272552 1108 38-39 0.7175346395107667 1108 40-41 0.6970251487782164 1108 42-43 0.739446756971823 1108 44-45 0.579743892295447 1108 46-47 0.786384754667381 1108 48-49 0.7251608827045999 1108 50-51 0.6224568894302038 1108 52-53 0.6933036122958356 1108 54-55 0.8199774626443954 1108 56-57 0.8686821858393401 1108 58-59 0.8096216040167903 1108 60-61 0.7247319191010533 1108 62-63 0.8208052278470248 1108 64-65 0.7801191180011244 1108 66-67 0.7294967929714673 1108 68-69 0.8228815222847459 1108 70-71 0.9642882999807583 1108 72-73 0.7887031650336667 1108 74-75 0.8582087082525369 1108 76-77 0.8724886758326633 1108 78-79 0.9128011993391354 1108 80-81 1.0000331955241535 1108 82-83 1.003908971229567 1108 84-85 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2316 88-89 -1.5717784453819483 2316 90-91 -1.8012892570914723 2316 92-93 -1.7172401137475255 2316 94-95 -1.7947854089821966 2316 96-97 -1.867071263944819 2316 98-99 -1.9265686759024518 2316 100-101 -1.8602169622493143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8675.0 3 2404.0 4 2649.0 5 2840.0 6 3434.0 7 4259.0 8 5182.0 9 6472.0 10 8065.0 11 10198.0 12 13719.0 13 19291.0 14 16987.0 15 17369.0 16 21508.0 17 28219.0 18 24868.0 19 18644.0 20 18990.0 21 20413.0 22 22301.0 23 24835.0 24 28227.0 25 32605.0 26 37512.0 27 43816.0 28 51622.0 29 61945.0 30 73870.0 31 89300.0 32 111305.0 33 142794.0 34 191733.0 35 273717.0 36 414115.0 37 618563.0 38 536457.0 39 82017.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.557744176701874 19.232252272486793 14.207594581734547 23.002408969076782 2 15.21405188017887 20.752071548148315 40.08428667182598 23.949589899846842 3 19.441853717175487 24.931961025098968 30.18883811063442 25.43734714709113 4 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22.360450769477104 24-25 22.343407986117015 26.686034584735292 29.124640373123057 21.845917056024643 26-27 22.05360998196551 26.445418351075585 29.610010460618547 21.890961206340357 28-29 21.67760467361803 26.984982744630244 29.524458976645505 21.812953605106216 30-31 21.927997176613815 26.69582870593721 29.176814559865825 22.199359557583158 32-33 21.851647045949452 26.78574319253599 29.21118047412183 22.15142928739273 34-35 22.401297586417858 26.670269270278034 29.031418922756608 21.897014220547497 36-37 22.477041028244603 26.311515665227788 29.30932149453311 21.90212181199451 38-39 21.99167892346072 26.90194293886772 29.184973305921453 21.921404831750106 40-41 22.045682771709636 26.841528847060047 29.285266274304618 21.8275221069257 42-43 21.758954266213824 26.952969124228837 28.756460050697026 22.531616558860318 44-45 22.010460031013036 26.82186947248093 28.74183932751231 22.425831168993728 46-47 22.4897647156544 27.11232423001094 28.25309090388935 22.14482015044531 48-49 22.258518739022914 27.08233178304051 28.284826189939295 22.37432328799728 50-51 21.95221595761301 27.279868603068735 28.605543076373085 22.162372362945167 52-53 22.237682703135846 27.54765071408401 28.058984579674068 22.15568200310608 54-55 22.068344551890597 27.22316974526766 28.14933468927024 22.55915101357151 56-57 22.207456404088997 27.461572215631147 27.87560741739936 22.455363962880497 58-59 22.38732218601199 27.040676716848246 28.102117193766063 22.469883903373706 60-61 22.187481473480585 27.248930639147662 28.494968501871533 22.06861938550022 62-63 22.057766532808476 27.08698983564147 28.57042313730092 22.284820494249125 64-65 21.910312571535595 27.460255473123425 28.335249620211016 22.29418233512996 66-67 22.03392669081028 27.327071907108504 27.85105972332676 22.78794167875446 68-69 21.78968306625141 27.267496923289077 28.347256588584557 22.59556342187496 70-71 22.236371299838233 27.123147601891244 28.04798884349476 22.592492254775763 72-73 22.11275261326308 26.844779432937887 28.275060196632307 22.767407757166726 74-75 21.81032929682241 27.78465922061671 27.816739499033854 22.58827198352703 76-77 22.023695638568768 27.197007688037502 27.918800539357402 22.860496134036328 78-79 22.024257137948965 27.154849680472577 28.123874023584943 22.697019157993516 80-81 22.191949765962292 27.230111426946575 27.92067666551648 22.65726214157465 82-83 22.11192191015318 27.136212017601164 28.144015378181052 22.607850694064606 84-85 22.01436021273089 27.18377021579006 27.879885526620786 22.92198404485827 86-87 21.857936909794155 27.151789219252 28.183309939799916 22.806963931153927 88-89 22.143935949427462 26.97546133640157 27.84391281738086 23.036689896790108 90-91 21.99225462828231 26.846183485970048 28.098758366838624 23.062803518909014 92-93 22.16905465463356 27.008587781605193 28.003211516192692 22.81914604756855 94-95 22.060447636936942 26.779760727832784 28.09379666678808 23.065994968442194 96-97 21.88353846376468 26.490010449596024 28.301268340650655 23.325182745988645 98-99 21.977851522931363 26.809087672003525 27.84710039006164 23.36596041500347 100-101 22.246363901185504 25.914047747855534 28.5060361060971 23.333552244861867 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2735.0 1 1633.0 2 618.0 3 706.5 4 628.0 5 691.5 6 1174.0 7 2180.0 8 3395.5 9 4034.5 10 3797.5 11 3565.5 12 3764.0 13 4042.0 14 4344.0 15 4669.5 16 4983.0 17 5209.0 18 5369.5 19 5639.0 20 6201.5 21 7119.5 22 8434.0 23 10168.5 24 11893.0 25 14523.0 26 19436.5 27 24917.0 28 28476.5 29 31513.5 30 35977.5 31 42187.5 32 49038.0 33 55737.0 34 63454.0 35 70365.5 36 79010.0 37 87161.0 38 90934.5 39 93172.0 40 93871.0 41 94176.5 42 93386.5 43 94329.5 44 97161.0 45 99625.5 46 102717.0 47 112266.5 48 136671.5 49 136277.0 50 111515.5 51 101508.5 52 100145.5 53 100026.0 54 97269.0 55 92166.0 56 85888.5 57 78394.0 58 71974.5 59 65566.5 60 55682.0 61 45072.0 62 35576.5 63 26847.0 64 19812.5 65 14560.0 66 10384.0 67 7827.5 68 5859.5 69 4101.0 70 2940.0 71 1904.5 72 1204.5 73 835.0 74 594.5 75 394.5 76 253.0 77 154.0 78 109.0 79 72.5 80 50.5 81 46.0 82 37.0 83 27.0 84 22.5 85 17.0 86 15.0 87 15.0 88 13.0 89 9.0 90 5.5 91 5.0 92 4.5 93 3.5 94 2.0 95 2.0 96 2.5 97 1.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3217154707482861 2 0.08605824137072013 3 0.09686405062553988 4 0.1014904899172741 5 0.11407569889968393 6 0.05658491133736448 7 0.0589143073443915 8 0.05994959445862572 9 0.06292604491204912 10-11 0.06041870893226311 12-13 0.05838048742611448 14-15 0.05640697136460547 16-17 0.058073136564076186 18-19 0.06642013892258965 20-21 0.06756866056494323 22-23 0.08303326183381701 24-25 0.09167143869320883 26-27 0.08672147217827643 28-29 0.08463472158864808 30-31 0.07894064246035983 32-33 0.07078775643576528 34-35 0.3178654967922276 36-37 0.4943657734080034 38-39 0.4660409650170013 40-41 0.4477616769063033 42-43 0.4792246993623278 44-45 0.4893834541707512 46-47 0.4829776151514269 48-49 0.5011274923728457 50-51 0.49705104936054845 52-53 0.49650105308111153 54-55 0.4876849362489607 56-57 0.4652321469590059 58-59 0.44769697146166365 60-61 0.40675460136593194 62-63 0.47846441038781207 64-65 0.5026965994053569 66-67 0.5130656469088591 68-69 0.5246964505828342 70-71 0.5219141164633299 72-73 0.5111568362919897 74-75 0.5122082997673839 76-77 0.48682758910748547 78-79 0.4669630176031162 80-81 0.47265709673140444 82-83 0.48152174264703507 84-85 0.4830584969572264 86-87 0.48326878965230535 88-89 0.4888658106136341 90-91 0.5029230684615957 92-93 0.5094583183701993 94-95 0.5156376883332847 96-97 0.5076627422814493 98-99 0.5103641945951541 100-101 0.4887202233631949 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3090930.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.6433751050544 #Duplication Level Percentage of deduplicated Percentage of total 1 71.00656661363581 30.989662216409403 2 14.233659470582904 12.424098787845196 3 5.387572404253596 7.053955300334386 4 2.8308357631683276 4.941889082910332 5 1.6294657699875104 3.5557692910205607 6 1.0170079994606265 2.663139696318065 7 0.7035283604647984 2.149304649296638 8 0.5071653989340228 1.7707527796785694 9 0.3742518809687803 1.4700253702403383 >10 2.048098578762688 16.164823718995 >50 0.1638867024887805 4.936523087019952 >100 0.09050761330694959 7.144661656134196 >500 0.005142876349871573 1.4983016383928622 >1k 0.0019378954360292234 1.8393067636645155 >5k 2.2360331954183343E-4 0.673552620986461 >10k+ 1.4906887969455565E-4 0.7242333407535287 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11193 0.3621240209257408 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11071 0.3581769888027228 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9001 0.2912068536006962 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5963 0.19291928319308427 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5742 0.18576933156040415 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4672 0.1511519186781972 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4118 0.13322851051301712 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4068 0.13161087439702612 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3491 0.11294335361849023 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3200 0.10352871142342274 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.23527223198196E-5 3.23527223198196E-5 2 0.0 0.0 0.0 3.23527223198196E-5 6.47054446396392E-5 3 0.0 0.0 0.0 3.23527223198196E-5 6.47054446396392E-5 4 0.0 0.0 0.0 3.23527223198196E-5 6.47054446396392E-5 5 0.0 0.0 0.0 3.23527223198196E-5 6.47054446396392E-5 6 0.0 0.0 0.0 6.47054446396392E-5 6.47054446396392E-5 7 3.23527223198196E-5 0.0 0.0 6.47054446396392E-5 6.47054446396392E-5 8 6.47054446396392E-5 0.0 0.0 6.47054446396392E-5 6.47054446396392E-5 9 6.47054446396392E-5 0.0 0.0 6.47054446396392E-5 6.47054446396392E-5 10-11 6.47054446396392E-5 0.0 0.0 1.6176361159909802E-4 6.47054446396392E-5 12-13 6.47054446396392E-5 0.0 0.0 2.2646905623873722E-4 6.47054446396392E-5 14-15 6.47054446396392E-5 0.0 0.0 2.2646905623873722E-4 1.1323452811936861E-4 16-17 8.088180579954901E-5 0.0 0.0 2.588217785585568E-4 1.7793997275900782E-4 18-19 9.705816695945881E-5 0.0 0.0 3.3970358435810586E-4 1.9411633391891762E-4 20-21 9.705816695945881E-5 0.0 0.0 5.176435571171136E-4 2.4264541739864702E-4 22-23 9.705816695945881E-5 0.0 0.0 7.602889745157606E-4 2.9117450087837643E-4 24-25 2.588217785585568E-4 0.0 0.0 9.544053084346783E-4 3.5587994551801563E-4 26-27 2.588217785585568E-4 0.0 0.0 0.001552930671351341 4.85290834797294E-4 28-29 2.588217785585568E-4 0.0 0.0 0.003995561206497721 4.85290834797294E-4 30-31 2.588217785585568E-4 0.0 0.0 0.010886691060619295 4.85290834797294E-4 32-33 2.588217785585568E-4 0.0 0.0 0.025720414244256584 5.176435571171136E-4 34-35 3.073508620382862E-4 0.0 0.0 0.04846437803508977 5.823490017567529E-4 36-37 3.8823266783783524E-4 0.0 0.0 0.0866729430947967 5.823490017567529E-4 38-39 4.2058539015765484E-4 0.0 0.0 0.1398446422274202 5.985253629166626E-4 40-41 4.2058539015765484E-4 0.0 0.0 0.20142804916319684 6.147017240765724E-4 42-43 4.85290834797294E-4 0.0 0.0 0.26828494983710405 6.147017240765724E-4 44-45 4.85290834797294E-4 0.0 0.0 0.34832558485633774 6.147017240765724E-4 46-47 5.014671959572038E-4 0.0 0.0 0.44573963176131454 6.308780852364822E-4 48-49 5.176435571171136E-4 0.0 0.0 0.5513874464966855 6.794071687162116E-4 50-51 5.176435571171136E-4 0.0 0.0 0.6644925637267748 7.117598910360313E-4 52-53 5.176435571171136E-4 0.0 0.0 0.7824344129436771 7.441126133558508E-4 54-55 5.176435571171136E-4 0.0 0.0 0.9183481994092393 8.0881805799549E-4 56-57 5.176435571171136E-4 0.0 0.0 1.0841882540206345 8.0881805799549E-4 58-59 5.176435571171136E-4 0.0 0.0 1.2658649662075816 8.0881805799549E-4 60-61 5.176435571171136E-4 0.0 0.0 1.4642033303892354 8.0881805799549E-4 62-63 5.176435571171136E-4 0.0 0.0 1.6705004642615653 8.0881805799549E-4 64-65 5.176435571171136E-4 0.0 0.0 1.8876195837498746 8.0881805799549E-4 66-67 5.176435571171136E-4 0.0 0.0 2.1364120183892874 8.411707803153096E-4 68-69 5.176435571171136E-4 0.0 0.0 2.4193527514372697 8.735235026351292E-4 70-71 5.176435571171136E-4 0.0 0.0 2.7325756325766033 8.735235026351292E-4 72-73 5.176435571171136E-4 0.0 0.0 3.0510396547317473 0.0010191107530743175 74-75 5.176435571171136E-4 1.61763611599098E-5 0.0 3.3932182223473193 0.0011646980035135055 76-77 5.176435571171136E-4 3.23527223198196E-5 0.0 3.7612466150964274 0.0012294034481531448 78-79 5.176435571171136E-4 3.23527223198196E-5 0.0 4.187865787966729 0.0012617561704729645 80-81 5.176435571171136E-4 3.23527223198196E-5 0.0 4.655928798128718 0.0012941088927927841 82-83 5.176435571171136E-4 3.23527223198196E-5 0.0 5.124800626348704 0.0013426379762725134 84-85 5.176435571171136E-4 3.23527223198196E-5 0.0 5.6123561517083855 0.0013588143374324232 86-87 5.176435571171136E-4 3.23527223198196E-5 0.0 6.135936433371186 0.001374990698592333 88-89 5.176435571171136E-4 3.23527223198196E-5 0.0 6.693341486219358 0.0013911670597522429 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11070 0.0 24.267801 1 TACATGG 11195 0.0 23.39669 2 ACATGGG 11125 0.0 22.522106 3 GTATAGG 1250 0.0 22.02316 1 GAGTACT 9675 0.0 21.433182 12-13 GTACTTT 10260 0.0 19.935217 14-15 AGTACTT 10460 0.0 19.86993 12-13 CATGGGA 6100 0.0 18.557583 4 CATGGGG 5345 0.0 18.431852 4 TACCGTA 130 0.0077102594 18.209463 7 GTATACG 135 0.009445363 17.579151 1 TTTGCGT 625 0.0 17.430153 3 GTACCGT 275 6.120754E-7 17.21622 6 ACCGTAT 170 0.001828112 16.709862 8 ATGGGAT 2815 0.0 16.489256 5 AGTACAT 8235 0.0 16.392101 2 ACTTTTT 12730 0.0 16.385052 16-17 GTATCAA 29330 0.0 16.215014 1 AGAGTAC 19915 0.0 16.058762 10-11 TACTTTT 12850 0.0 15.825033 14-15 >>END_MODULE