FastQCFastQC Report
Fri 27 May 2016
SRR938999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090930
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT117730.3808885998712362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109540.3543917202913039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98700.3193213692966195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61310.19835454054281396No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55080.17819879453756637No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43420.14047552031265673No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41380.1338755649594135No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39530.1278903113302469No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35320.11426981523360283No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31750.10271989336542724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118450.023.766141
TACATGG119350.023.1394312
ACATGGG117700.022.3752883
GAGTACT103300.021.68242512-13
GTACTTT112100.019.93799414-15
CATGGGG55550.019.0490324
AGTACTT112450.018.90535212-13
GTACCGT3054.629328E-918.6695376
CATGGGA62450.018.2360554
TATAGCG1952.2946234E-417.0339575
TTTGCGT6700.016.9976373
GTACGGG2253.5717152E-516.8717296
ATAGCGC1700.001800687616.7476718
AGTACAT89350.016.6227132
ACTTTTT134000.016.5728316-17
CCTATAC9850.016.379283
AGAGTAC207400.016.26773310-11
GTATAGG10500.015.8776041
TAGCGTG1800.002637777515.8172457
TACTTTT135850.015.561614-15
Produced by FastQC (version 0.11.5)