##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938999_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3090930 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.182239002500864 33.0 31.0 34.0 30.0 34.0 2 32.41944851549534 34.0 31.0 34.0 31.0 34.0 3 32.50736250901832 34.0 31.0 34.0 30.0 34.0 4 36.059000042058535 37.0 35.0 37.0 35.0 37.0 5 35.98111571598192 37.0 35.0 37.0 35.0 37.0 6 36.02563791480234 37.0 35.0 37.0 35.0 37.0 7 35.96282122209173 37.0 35.0 37.0 35.0 37.0 8 35.9358891983966 37.0 35.0 37.0 35.0 37.0 9 37.610435370584256 39.0 37.0 39.0 35.0 39.0 10-11 37.52418738049713 39.0 37.0 39.0 35.0 39.0 12-13 37.435787449084906 39.0 37.0 39.0 34.0 39.0 14-15 38.74438081742388 40.0 38.0 41.0 34.5 41.0 16-17 38.83557618580815 40.0 38.0 41.0 35.0 41.0 18-19 38.88120598007719 40.0 38.0 41.0 35.0 41.0 20-21 38.8529969944321 40.0 38.0 41.0 34.5 41.0 22-23 38.77644835049645 40.0 38.0 41.0 34.0 41.0 24-25 38.69996360318739 40.0 38.0 41.0 34.0 41.0 26-27 38.533722051292 40.0 38.0 41.0 34.0 41.0 28-29 38.38174044704992 40.0 38.0 41.0 34.0 41.0 30-31 38.19198865713555 40.0 38.0 41.0 33.5 41.0 32-33 38.093069238061034 40.0 37.5 41.0 33.0 41.0 34-35 37.99587810141284 40.0 37.0 41.0 33.0 41.0 36-37 37.86026778348264 40.0 37.0 41.0 33.0 41.0 38-39 37.71334598324776 40.0 37.0 41.0 33.0 41.0 40-41 37.54992235346643 40.0 37.0 41.0 32.5 41.0 42-43 37.362209270349055 40.0 37.0 41.0 32.0 41.0 44-45 37.110530164060656 40.0 36.0 41.0 31.0 41.0 46-47 36.794058422545966 40.0 36.0 41.0 30.5 41.0 48-49 36.451934369267505 39.0 36.0 41.0 30.0 41.0 50-51 36.18696298525039 39.0 35.0 41.0 29.5 41.0 52-53 35.92232062841928 39.0 35.0 40.0 29.0 41.0 54-55 35.62133095864351 38.5 35.0 40.0 28.0 41.0 56-57 35.477671606927366 38.0 35.0 40.0 28.0 41.0 58-59 35.197192916047925 38.0 34.0 40.0 27.5 41.0 60-61 34.825148094586424 38.0 34.0 40.0 26.5 41.0 62-63 34.402785245864514 37.0 34.0 40.0 26.0 41.0 64-65 33.984974586936616 36.5 33.0 39.5 26.0 41.0 66-67 33.521219018224286 36.0 33.0 39.0 25.0 40.0 68-69 33.01808614235845 35.5 32.0 39.0 23.5 40.0 70-71 32.48355931709874 35.0 32.0 38.0 22.0 40.0 72-73 31.96511842714005 35.0 31.0 37.0 21.5 39.0 74-75 31.435936271607574 35.0 31.0 36.5 20.0 39.0 76-77 29.536464915090278 32.5 28.5 35.0 17.5 37.0 78-79 30.503719916012333 34.0 30.0 35.5 18.0 37.5 80-81 30.481952519144723 34.0 30.0 35.0 17.5 37.0 82-83 30.071289385395332 34.0 30.0 35.0 12.5 37.0 84-85 29.61560080622984 34.0 29.5 35.0 7.0 36.0 86-87 28.92429139449939 34.0 29.0 35.0 2.0 36.0 88-89 28.21112982176885 33.0 27.5 35.0 2.0 35.0 90-91 27.618037289747747 33.0 26.5 35.0 2.0 35.0 92-93 27.200729715651924 33.0 26.0 34.0 2.0 35.0 94-95 26.62606561779141 32.0 25.0 34.0 2.0 35.0 96-97 25.746511891243088 32.0 23.5 34.0 2.0 35.0 98-99 24.494234259591774 32.0 10.5 34.0 2.0 35.0 100-101 18.84822658552604 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18068020457174327 1101 2 0.18275892388894732 1101 3 0.22853845065259293 1101 4 0.15135184136622826 1101 5 0.12674707353189518 1101 6 0.16068447379598183 1101 7 0.13628750092386355 1101 8 0.20298988339237667 1101 9 0.3022578475037534 1101 10-11 0.25156952142004485 1101 12-13 0.306737952462548 1101 14-15 0.3605571103459013 1101 16-17 0.33917949836150285 1101 18-19 0.4077115900949124 1101 20-21 0.3975470167943769 1101 22-23 0.3868498964898137 1101 24-25 0.28654918686111586 1101 26-27 0.21626024949596712 1101 28-29 0.32435819587357884 1101 30-31 0.22191708479432037 1101 32-33 0.37057119690941676 1101 34-35 0.41096921615018545 1101 36-37 0.5270469108749651 1101 38-39 0.43510268996562473 1101 40-41 0.4057523769586169 1101 42-43 0.18493882288529306 1101 44-45 0.3354225918205884 1101 46-47 0.342245698619827 1101 48-49 0.31429277951187373 1101 50-51 0.221666097829484 1101 52-53 0.18648411370142526 1101 54-55 0.2766257683800646 1101 56-57 0.18254905604150196 1101 58-59 0.18583270443227207 1101 60-61 0.22607795754549898 1101 62-63 0.21712153948968904 1101 64-65 0.2782979688866405 1101 66-67 0.07498474385786835 1101 68-69 0.1943119701421665 1101 70-71 0.2812618830645448 1101 72-73 0.3220800737946732 1101 74-75 0.25819390741907355 1101 76-77 -0.02880345160092901 1101 78-79 0.2607254162948216 1101 80-81 0.3370979576062396 1101 82-83 0.4249810769407354 1101 84-85 0.5019389838679089 1101 86-87 0.595695063547236 1101 88-89 0.7471472159746924 1101 90-91 0.6867907694869082 1101 92-93 0.608696662296424 1101 94-95 0.5256204251123329 1101 96-97 0.68071414262376 1101 98-99 1.0104694177841225 1101 100-101 1.3496368734975839 1102 1 0.10571065846719563 1102 2 0.15601540159434535 1102 3 0.0918843186327507 1102 4 0.1257941084307106 1102 5 0.0389412840551131 1102 6 0.12003220137632553 1102 7 0.15701380426231282 1102 8 0.13827461995882118 1102 9 0.14316493222737137 1102 10-11 0.23238650969161512 1102 12-13 0.2608062692301374 1102 14-15 0.2688570473784253 1102 16-17 0.27169543148651343 1102 18-19 0.24202327320962524 1102 20-21 0.2268595532066371 1102 22-23 0.2421975711068569 1102 24-25 0.3211481958589957 1102 26-27 0.26369844427143363 1102 28-29 0.3416006912687166 1102 30-31 0.34692959001708346 1102 32-33 0.31431167087207257 1102 34-35 0.3128326414631104 1102 36-37 0.3115936921777447 1102 38-39 0.3994216113975142 1102 40-41 0.3287628695761242 1102 42-43 0.49370086467921226 1102 44-45 0.4751485950336871 1102 46-47 0.44078317603686656 1102 48-49 0.4810719931834626 1102 50-51 0.4638827000574679 1102 52-53 0.5102266767718859 1102 54-55 0.5948189549462128 1102 56-57 0.6010282444084041 1102 58-59 0.46496545987719173 1102 60-61 0.5414920422514626 1102 62-63 0.5723309210549345 1102 64-65 0.5415689526504437 1102 66-67 0.39552082068995276 1102 68-69 0.35359834857079875 1102 70-71 0.4413051952714113 1102 72-73 0.5182436185692509 1102 74-75 0.4984729357191249 1102 76-77 0.2632942774506155 1102 78-79 0.61087606744913 1102 80-81 0.6011324955603286 1102 82-83 0.660622874397319 1102 84-85 0.7572691511730802 1102 86-87 0.8512937253412822 1102 88-89 0.7926287111874011 1102 90-91 0.8740682500817449 1102 92-93 1.027283248458879 1102 94-95 1.0645844924581063 1102 96-97 1.219314087306536 1102 98-99 1.374591345177329 1102 100-101 1.5200087641107238 1103 1 0.08287262547431595 1103 2 0.06902479800477579 1103 3 0.12021534685688806 1103 4 0.08104008612423286 1103 5 0.06908731308640625 1103 6 0.14111122517348917 1103 7 0.13193719755757627 1103 8 0.11332890273536833 1103 9 0.10530514976569094 1103 10-11 0.15784404114508277 1103 12-13 0.23372123615146023 1103 14-15 0.2684058422498907 1103 16-17 0.14896454607734455 1103 18-19 0.24195146578586701 1103 20-21 0.21437669609287013 1103 22-23 0.12303378764460149 1103 24-25 0.15120983753303818 1103 26-27 0.08405135867116087 1103 28-29 0.12549506782030306 1103 30-31 0.18876165008663293 1103 32-33 0.12750656676403338 1103 34-35 0.14215566652263334 1103 36-37 0.1578638146175848 1103 38-39 0.11568721087861178 1103 40-41 0.1123102072113582 1103 42-43 0.1600137661645178 1103 44-45 0.05805362889771004 1103 46-47 0.14938683250724694 1103 48-49 -0.04280442969427867 1103 50-51 0.008638176691519561 1103 52-53 0.027526448587892105 1103 54-55 0.2028231375923042 1103 56-57 0.35143945552876943 1103 58-59 0.24462138051745086 1103 60-61 0.2270200649622396 1103 62-63 0.22036474289689778 1103 64-65 0.32218800172382345 1103 66-67 0.18433355129177187 1103 68-69 0.11791583991391974 1103 70-71 0.23777241545514016 1103 72-73 0.1963009423447417 1103 74-75 0.1887127979652199 1103 76-77 0.050428350454954085 1103 78-79 0.1714637175902709 1103 80-81 0.24052433940045148 1103 82-83 0.1521503255573613 1103 84-85 0.31246888124988104 1103 86-87 0.5870109560688981 1103 88-89 0.357650787879912 1103 90-91 0.3244224152031876 1103 92-93 0.4367540841009614 1103 94-95 0.6102458333772134 1103 96-97 0.4028096872384772 1103 98-99 0.7184472838512441 1103 100-101 0.864209014836792 1104 1 0.10712441452347576 1104 2 0.07421920534831372 1104 3 0.031339811488713565 1104 4 0.08411616500314523 1104 5 0.0636076004788535 1104 6 0.05968476618212293 1104 7 0.06537522698189235 1104 8 0.1005835843927656 1104 9 0.10522267213774938 1104 10-11 0.14961816017039808 1104 12-13 0.11469213248970078 1104 14-15 0.18592316465577596 1104 16-17 0.1633236486197589 1104 18-19 0.18941742482815016 1104 20-21 0.20304990879004947 1104 22-23 0.13865067401314946 1104 24-25 0.21718604862864055 1104 26-27 0.14876599870260776 1104 28-29 0.18535236469179495 1104 30-31 0.06539952532828153 1104 32-33 0.15811502442397085 1104 34-35 0.13373789882171394 1104 36-37 0.09556080359503483 1104 38-39 0.09353018525752788 1104 40-41 0.22895545494301217 1104 42-43 0.14987869642463636 1104 44-45 0.2859372108842564 1104 46-47 0.32767400782522316 1104 48-49 0.1598287948190844 1104 50-51 0.15068905865508953 1104 52-53 0.15954998669297282 1104 54-55 0.32836923627426273 1104 56-57 0.19503494147770084 1104 58-59 0.23807787483112008 1104 60-61 0.2038762534353964 1104 62-63 0.1820601967805402 1104 64-65 0.23054020812660525 1104 66-67 0.16205785887945723 1104 68-69 0.01475568435101593 1104 70-71 0.12017572937257626 1104 72-73 0.06940742853689841 1104 74-75 0.06205577613136981 1104 76-77 0.07272003342778177 1104 78-79 0.24766631294240327 1104 80-81 0.3249380875794188 1104 82-83 0.22372533685595286 1104 84-85 0.25867663566180354 1104 86-87 0.37299497729089737 1104 88-89 0.48958385307679464 1104 90-91 0.5671342964421378 1104 92-93 0.6046726433346237 1104 94-95 0.8010254236990555 1104 96-97 0.8012191314318393 1104 98-99 1.1295124150874578 1104 100-101 1.2661010347896493 1105 1 0.02564124516852928 1105 2 -0.04305580759364602 1105 3 3.5972403252770846E-4 1105 4 0.035692126896769594 1105 5 -0.05617366989328332 1105 6 0.0040043149431454594 1105 7 0.025676860226312215 1105 8 0.0579374318029906 1105 9 0.01883176731779912 1105 10-11 0.039932320008610134 1105 12-13 0.0514782421915001 1105 14-15 0.00998665040930291 1105 16-17 -0.038260115994795285 1105 18-19 0.05605812935870347 1105 20-21 0.0726474925513827 1105 22-23 0.0030623230413695524 1105 24-25 -0.07680569198591769 1105 26-27 -0.1317513228685243 1105 28-29 0.08892956610337421 1105 30-31 0.07678662852178775 1105 32-33 0.005964664935611097 1105 34-35 -0.011285877770312425 1105 36-37 0.11032847963262782 1105 38-39 0.0671319376665096 1105 40-41 0.12381244104173561 1105 42-43 0.19357563498894592 1105 44-45 0.24711914358420728 1105 46-47 0.2357747926968159 1105 48-49 0.2347129742802636 1105 50-51 0.1815037049606829 1105 52-53 0.13400986240326063 1105 54-55 0.15055326362344346 1105 56-57 0.15649501477869165 1105 58-59 0.11637156173761554 1105 60-61 0.1426766384791449 1105 62-63 0.21532671613809384 1105 64-65 0.06722779234319631 1105 66-67 0.038391244152713 1105 68-69 0.14866897300662885 1105 70-71 0.21636532633915806 1105 72-73 0.1383127290836299 1105 74-75 0.0021517648522753063 1105 76-77 -0.1515339208503832 1105 78-79 0.026726872382244693 1105 80-81 0.07802555971842651 1105 82-83 0.012177908280772698 1105 84-85 0.04397086512140547 1105 86-87 0.23519534694039734 1105 88-89 0.3523860295783301 1105 90-91 0.3823983663319872 1105 92-93 0.6754178606773635 1105 94-95 0.6100546502276494 1105 96-97 0.794199029276033 1105 98-99 0.7644746123712167 1105 100-101 0.6456166981945834 1106 1 -0.04766107440244838 1106 2 0.04509981787541051 1106 3 -0.024457498545885414 1106 4 -0.01252085415203652 1106 5 0.01095323305451501 1106 6 0.03933465624363208 1106 7 0.040736427524691976 1106 8 0.07282925639630378 1106 9 0.02300545432731127 1106 10-11 0.13935280002610995 1106 12-13 0.053062478850364414 1106 14-15 -0.03316484983175627 1106 16-17 -0.16684980988299003 1106 18-19 -0.06120644686109955 1106 20-21 -0.03777504510996721 1106 22-23 -0.006391531561739328 1106 24-25 0.14909030256679756 1106 26-27 0.064008141547653 1106 28-29 -0.026210890454997582 1106 30-31 0.05564172864554706 1106 32-33 0.059208684597543026 1106 34-35 0.013110745506907051 1106 36-37 -0.07459107842124979 1106 38-39 -0.02250914264620718 1106 40-41 0.0936597579039784 1106 42-43 0.15744820520479408 1106 44-45 0.24013918324603623 1106 46-47 0.18596825594998023 1106 48-49 0.26965440101273686 1106 50-51 0.17331413333827328 1106 52-53 0.2270337539346059 1106 54-55 0.2702836576075711 1106 56-57 0.24405049412521151 1106 58-59 0.13964120254456702 1106 60-61 0.24161252845981096 1106 62-63 0.33803174742521236 1106 64-65 0.15715838413945704 1106 66-67 0.16648765023419543 1106 68-69 0.05943296026315181 1106 70-71 0.08783962318658212 1106 72-73 0.24653659477018408 1106 74-75 0.17522993046775426 1106 76-77 0.031949262632796405 1106 78-79 0.2709360214421359 1106 80-81 0.28276919916356036 1106 82-83 0.18338915971590453 1106 84-85 0.2639772269188079 1106 86-87 0.43017387587415 1106 88-89 0.46862861783584364 1106 90-91 0.5392477358679244 1106 92-93 0.5832985372147519 1106 94-95 0.7762112548170883 1106 96-97 0.8043132931216412 1106 98-99 0.8981021788346055 1106 100-101 0.9425734727632964 1107 1 0.052913972441253065 1107 2 0.08433752875491507 1107 3 0.030956738957904406 1107 4 0.033687001011045936 1107 5 0.05837479987906846 1107 6 0.03772962777293287 1107 7 0.039365990333358525 1107 8 0.04256731270152869 1107 9 0.05358979535941444 1107 10-11 0.05919961987292055 1107 12-13 -0.0011902336113038814 1107 14-15 0.05298001691013354 1107 16-17 0.0587887306809165 1107 18-19 0.02769238233624094 1107 20-21 0.047451502803149026 1107 22-23 0.09395256878235614 1107 24-25 0.07264402910559653 1107 26-27 0.11578372914413393 1107 28-29 0.11273855147952361 1107 30-31 0.12521494150234247 1107 32-33 0.19363992499892646 1107 34-35 0.15342466044769054 1107 36-37 0.2412496314493069 1107 38-39 0.15272292213809635 1107 40-41 0.1838229943358698 1107 42-43 0.2169323363307214 1107 44-45 0.11344282819147367 1107 46-47 0.16023958992280996 1107 48-49 0.15952075356565132 1107 50-51 0.07254094301284653 1107 52-53 0.027617581384106415 1107 54-55 -0.007704689037488777 1107 56-57 0.08430294482542422 1107 58-59 -5.011774376129097E-5 1107 60-61 0.018765889195265117 1107 62-63 0.06528525676827712 1107 64-65 0.09571037940786908 1107 66-67 0.11070015414818357 1107 68-69 0.11740483913055755 1107 70-71 0.2939169620997433 1107 72-73 0.156637770543 1107 74-75 0.06919533488092 1107 76-77 0.07884449012504291 1107 78-79 0.39547781464064613 1107 80-81 0.34065559137830803 1107 82-83 0.1752828381767486 1107 84-85 0.469449532391117 1107 86-87 0.45082552936162656 1107 88-89 0.6879694759826691 1107 90-91 0.647564957617984 1107 92-93 0.6571154334197118 1107 94-95 0.7890196735956572 1107 96-97 0.9306380463120654 1107 98-99 0.7676707528807931 1107 100-101 1.0578132909881894 1108 1 0.06565413342495674 1108 2 0.07566139721748044 1108 3 0.13719081249266907 1108 4 0.038259666140682214 1108 5 0.03774926899689035 1108 6 0.09271081937589543 1108 7 0.14153603442185414 1108 8 0.12466495457346838 1108 9 0.21003116574326697 1108 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-0.8385605945981283 2316 50-51 -0.8628495148920194 2316 52-53 -0.8212092777806106 2316 54-55 -0.7139238396340559 2316 56-57 -0.8667553443076059 2316 58-59 -0.8497115570312488 2316 60-61 -0.7190772341649136 2316 62-63 -0.5781964300230626 2316 64-65 -0.5065251508152429 2316 66-67 -0.5864035506548575 2316 68-69 -0.5752578886174291 2316 70-71 -0.5442252124525595 2316 72-73 -0.5452370709620986 2316 74-75 -0.7824094023395425 2316 76-77 -0.6677196969125383 2316 78-79 -0.670156479177372 2316 80-81 -0.6626145400927967 2316 82-83 -0.6754821490344689 2316 84-85 -0.9250113353565048 2316 86-87 -0.9251947544205699 2316 88-89 -1.1022638507991687 2316 90-91 -1.2659150311330585 2316 92-93 -1.1463081456303073 2316 94-95 -1.0534205484777068 2316 96-97 -1.0429491819290604 2316 98-99 -1.0035828064300638 2316 100-101 -0.3738580129775144 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 15.0 5 53.0 6 118.0 7 350.0 8 862.0 9 1771.0 10 2756.0 11 4336.0 12 6523.0 13 8779.0 14 11044.0 15 14654.0 16 19088.0 17 26973.0 18 26889.0 19 19108.0 20 17933.0 21 19480.0 22 21168.0 23 23667.0 24 27267.0 25 32395.0 26 38219.0 27 46716.0 28 56849.0 29 68255.0 30 82362.0 31 100305.0 32 124086.0 33 158488.0 34 214010.0 35 307704.0 36 475669.0 37 688718.0 38 423232.0 39 21077.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42488116909796 20.006950410122915 14.76595143482123 22.802216985957898 2 15.594173921764648 21.28424131248524 39.44033025658944 23.681254509160674 3 19.43880967864041 25.097915514100933 30.44077348888522 25.022501318373436 4 12.972212246799508 17.259497950455042 37.33455626623702 32.43373353650843 5 13.198357775815046 37.95275208432414 34.74158263047044 14.107307509390377 6 31.182168473566207 36.888250461835106 17.262894986298623 14.666686078300058 7 27.646080629454566 33.35478318823137 21.25386210622693 17.745274076087135 8 25.549559517685616 35.27501431607963 19.855836269342884 19.319589896891873 9 25.781755005774958 17.27250374482761 21.084107372214834 35.8616338771826 10-11 24.731229759328098 26.6501506019224 27.47352738496181 21.145092253787695 12-13 25.913430585616627 24.247718324258393 27.851924825214418 21.98692626491056 14-15 22.740335992535513 25.656394152904234 26.48962988797445 25.113639966585804 16-17 21.52714328975533 28.814930232919295 27.414444412809914 22.24348206451546 18-19 21.46528384056962 27.770015786579805 29.29466066132812 21.470039711522457 20-21 22.40272345216488 26.600068587771318 29.200790700533496 21.796417259530305 22-23 22.009637875979074 26.546185128747656 29.186862853574812 22.257314141698455 24-25 22.258342280219214 26.81492102772421 29.211581058300805 21.71515563375577 26-27 22.022142203155685 26.54610424694186 29.683946255657684 21.747807294244776 28-29 21.595150973978704 27.10430194148687 29.58895866292669 21.711588421607736 30-31 21.896370995137385 26.751479328228072 29.27520196186908 22.07694771476546 32-33 21.826618525815856 26.8320052540821 29.311922301702076 22.029453918399962 34-35 22.379265898872415 26.699253189125592 29.170017616653908 21.75146329534808 36-37 22.435278540812046 26.360610507236704 29.41607531848017 21.788035633471075 38-39 21.967616241869482 26.947599853426162 29.295644767144164 21.78913913756019 40-41 21.972736236474688 26.86364801871401 29.36106570206254 21.802550042748766 42-43 21.713346789529275 27.030048665874308 28.817807345387614 22.438797199208803 44-45 22.01222985237697 26.858698309008812 28.74065456126646 22.38841727734776 46-47 22.417093673100826 27.230921240364452 28.287652973343018 22.0643321131917 48-49 22.288906859589815 27.1695482788057 28.294794915352124 22.24674994625236 50-51 21.94812310341263 27.370322721745527 28.575338649884575 22.106215524957264 52-53 22.201149815751247 27.711805184847275 27.9592226287881 22.127822370613377 54-55 22.07854917452029 27.285833066423375 28.06119840954017 22.574419349516166 56-57 22.21805411316335 27.49873015564895 27.864105625167827 22.419110106019872 58-59 22.3735047264459 27.14982273805803 28.03694127223539 22.439731263260676 60-61 22.144349895847 27.37320745139217 28.42476694785362 22.05767570490722 62-63 22.02121722419307 27.237749058926493 28.507757486933354 22.233276229947087 64-65 21.85627216887484 27.566865944903828 28.255014025829656 22.321847860391674 66-67 21.992736291736357 27.3890914314546 27.812325138865724 22.80584713794332 68-69 21.76117935005039 27.398891245302675 28.193768390946843 22.64616101370009 70-71 22.188672432895164 27.275768430101184 27.94103778201275 22.594521354990903 72-73 22.104932819572102 26.986505679520402 28.05817343000327 22.850388070904227 74-75 21.7037752391675 27.917180266133496 27.598020660448476 22.781023834250533 76-77 21.921354265246112 27.291901806897002 27.82591265711928 22.960831270737607 78-79 21.977402811609966 27.2589666131427 27.950173206070737 22.8134573691766 80-81 22.04818920447808 27.389268019025398 27.72664324430214 22.83589953219437 82-83 21.975149873986147 27.23448929610182 28.011067866305613 22.779292963606423 84-85 21.8163459455752 27.305332981982254 27.778184883459662 23.10013618898288 86-87 21.656064155849624 27.244588509781885 28.013652385524455 23.085694948844033 88-89 21.855108760706948 27.081052119230304 27.71407887904957 23.349760241013183 90-91 21.731790796938526 26.918381603360125 27.91960888174122 23.430218717960127 92-93 21.853681578036387 27.191929289890098 27.720313950817392 23.234075181256127 94-95 21.706489743037668 26.892373274390422 27.850880350240974 23.550256632330935 96-97 21.45294458302194 26.722588347196474 28.03695004416147 23.78751702562012 98-99 21.464332741278515 26.997068843357823 27.602032398016128 23.93656601734753 100-101 21.610643954112028 26.077697280323424 28.50634390613277 23.805314859431775 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1067.0 1 725.5 2 463.0 3 562.5 4 522.0 5 547.5 6 919.5 7 1903.5 8 2996.0 9 3525.5 10 3331.5 11 2977.5 12 3092.5 13 3339.0 14 3557.5 15 3799.0 16 4054.0 17 4353.5 18 4707.5 19 5101.0 20 5788.5 21 6854.0 22 8229.0 23 10048.5 24 11912.0 25 14678.5 26 19721.0 27 25246.0 28 28736.0 29 32004.5 30 36525.0 31 42620.5 32 49445.0 33 56252.5 34 64106.0 35 70891.0 36 79119.5 37 87099.5 38 91383.0 39 94018.0 40 94349.5 41 94219.0 42 93716.5 43 94742.0 44 97880.5 45 100519.0 46 103289.0 47 113517.5 48 137714.5 49 136889.5 50 113451.5 51 103421.5 52 100393.0 53 100007.5 54 97856.5 55 92304.0 56 86145.0 57 79179.0 58 72383.5 59 65469.5 60 55227.5 61 44537.5 62 35033.5 63 26256.5 64 19248.5 65 13924.5 66 9777.0 67 7386.5 68 5472.0 69 3770.0 70 2586.5 71 1592.0 72 1054.0 73 751.5 74 494.0 75 307.5 76 185.5 77 81.0 78 37.5 79 22.0 80 12.5 81 9.5 82 8.5 83 5.0 84 2.5 85 2.5 86 1.5 87 1.5 88 1.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38742384977983974 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.058762249549489E-4 16-17 0.0023941014516666503 18-19 4.3676175131756467E-4 20-21 0.0 22-23 0.0 24-25 1.1323452811936861E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012569032621249915 36-37 0.024118954489425512 38-39 0.010919043782939116 40-41 0.006389662658164371 42-43 0.005192611932331046 44-45 0.0016338124771508899 46-47 0.001035287114234227 48-49 0.0031220377038625917 50-51 5.338199182770234E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.764653356756705E-4 60-61 0.0014396961432319721 62-63 0.0014558725043918822 64-65 8.249944191553997E-4 66-67 0.0027338050360247563 68-69 0.0024264541739864704 70-71 4.8529083479729405E-5 72-73 0.0 74-75 0.0 76-77 0.0902802716334566 78-79 0.0033323303989414186 80-81 3.073508620382862E-4 82-83 0.0 84-85 1.4558725043918821E-4 86-87 0.09139644055349037 88-89 0.4288676870715286 90-91 0.3804356617587587 92-93 0.0 94-95 9.544053084346783E-4 96-97 0.0 98-99 0.0 100-101 3.5587994551801563E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3090930.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.94547068570105 #Duplication Level Percentage of deduplicated Percentage of total 1 69.049977667308 28.963338140923806 2 15.259719441259477 12.801522289907439 3 5.815259828452371 7.3177143199225165 4 2.899742032594989 4.865241776972332 5 1.7598153363961728 3.6908141302526407 6 1.075491868315163 2.7067207581074118 7 0.7265686818385781 2.133338574164598 8 0.534647488774318 1.7940832454053468 9 0.4066104865077253 1.534992141807759 >10 2.188302471099445 16.673783916546242 >50 0.17862193475328894 5.186111680229464 >100 0.09735146113390164 7.463167557655861 >500 0.0051835020096166655 1.4355529224214132 >1k 0.0023209710488819673 1.9980980747656454 >5k 2.3209710488819674E-4 0.6979973281708287 >10k+ 1.5473140325879782E-4 0.737523142746684 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11773 0.3808885998712362 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10954 0.3543917202913039 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9870 0.3193213692966195 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6131 0.19835454054281396 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5508 0.17819879453756637 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4342 0.14047552031265673 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4138 0.1338755649594135 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3953 0.1278903113302469 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3532 0.11426981523360283 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3175 0.10271989336542724 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.23527223198196E-5 2 0.0 0.0 0.0 0.0 3.23527223198196E-5 3 0.0 0.0 0.0 3.23527223198196E-5 3.23527223198196E-5 4 0.0 0.0 0.0 3.23527223198196E-5 9.705816695945881E-5 5 0.0 0.0 0.0 3.23527223198196E-5 9.705816695945881E-5 6 3.23527223198196E-5 0.0 0.0 3.23527223198196E-5 9.705816695945881E-5 7 3.23527223198196E-5 0.0 0.0 3.23527223198196E-5 9.705816695945881E-5 8 3.23527223198196E-5 0.0 0.0 3.23527223198196E-5 9.705816695945881E-5 9 3.23527223198196E-5 0.0 0.0 9.705816695945881E-5 9.705816695945881E-5 10-11 3.23527223198196E-5 0.0 0.0 2.2646905623873722E-4 9.705816695945881E-5 12-13 6.47054446396392E-5 0.0 0.0 2.9117450087837643E-4 1.9411633391891762E-4 14-15 6.47054446396392E-5 0.0 0.0 2.9117450087837643E-4 3.3970358435810586E-4 16-17 6.47054446396392E-5 0.0 0.0 3.3970358435810586E-4 4.2058539015765484E-4 18-19 6.47054446396392E-5 0.0 0.0 4.5293811247747444E-4 4.2058539015765484E-4 20-21 6.47054446396392E-5 0.0 0.0 6.632308075563019E-4 4.2058539015765484E-4 22-23 9.705816695945881E-5 0.0 0.0 8.735235026351292E-4 5.014671959572038E-4 24-25 9.705816695945881E-5 0.0 0.0 0.0010514634753941371 5.66172640596843E-4 26-27 9.705816695945881E-5 0.0 0.0 0.0016338124771508899 5.985253629166626E-4 28-29 9.705816695945881E-5 0.0 0.0 0.00407644301229727 6.147017240765724E-4 30-31 9.705816695945881E-5 0.0 0.0 0.011113160116858034 6.147017240765724E-4 32-33 9.705816695945881E-5 0.0 0.0 0.02602776510629487 6.147017240765724E-4 34-35 1.294108892792784E-4 0.0 0.0 0.049176137926125796 6.147017240765724E-4 36-37 1.9411633391891762E-4 0.0 0.0 0.08793469926526967 6.147017240765724E-4 38-39 2.2646905623873722E-4 0.0 0.0 0.14233580184604633 6.147017240765724E-4 40-41 2.2646905623873722E-4 0.0 0.0 0.20471185047865853 6.147017240765724E-4 42-43 3.2352722319819603E-4 0.0 0.0 0.2724422746552009 6.147017240765724E-4 44-45 3.5587994551801563E-4 0.0 0.0 0.35345349134402915 6.308780852364822E-4 46-47 3.5587994551801563E-4 0.0 0.0 0.4516763563070014 6.955835298761214E-4 48-49 3.8823266783783524E-4 0.0 0.0 0.5583756345177665 7.117598910360313E-4 50-51 3.8823266783783524E-4 0.0 0.0 0.6726778024736891 7.117598910360313E-4 52-53 3.8823266783783524E-4 0.0 0.0 0.7913475879427874 7.27936252195941E-4 54-55 3.8823266783783524E-4 0.0 0.0 0.928070192466345 7.441126133558508E-4 56-57 3.8823266783783524E-4 0.0 0.0 1.0951720032482133 8.088180579954901E-4 58-59 3.8823266783783524E-4 0.0 0.0 1.2782398824949126 8.411707803153097E-4 60-61 4.2058539015765484E-4 0.0 0.0 1.4781797064313977 8.411707803153097E-4 62-63 4.2058539015765484E-4 0.0 0.0 1.6863047691147972 9.058762249549489E-4 64-65 4.2058539015765484E-4 0.0 0.0 1.905332699219976 9.382289472747684E-4 66-67 4.2058539015765484E-4 0.0 0.0 2.156972173423533 9.544053084346782E-4 68-69 4.2058539015765484E-4 0.0 0.0 2.4421128915892627 9.70581669594588E-4 70-71 4.2058539015765484E-4 0.0 0.0 2.758457810432459 0.0010352871142342273 72-73 4.2058539015765484E-4 0.0 0.0 3.079671813984788 0.0011161689200337762 74-75 4.2058539015765484E-4 0.0 0.0 3.424648892081024 0.001132345281193686 76-77 4.2058539015765484E-4 0.0 0.0 3.7948287408644 0.001132345281193686 78-79 4.2058539015765484E-4 0.0 0.0 4.222386142681976 0.001132345281193686 80-81 4.2058539015765484E-4 0.0 0.0 4.690999149123403 0.0011485216423535959 82-83 4.2058539015765484E-4 0.0 0.0 5.162232726072736 0.0011970507258333252 84-85 4.2058539015765484E-4 0.0 0.0 5.65095294943593 0.0011970507258333252 86-87 4.2058539015765484E-4 0.0 0.0 6.177833208775352 0.0011970507258333252 88-89 4.2058539015765484E-4 0.0 0.0 6.737227954046194 0.0011970507258333252 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11845 0.0 23.76614 1 TACATGG 11935 0.0 23.139431 2 ACATGGG 11770 0.0 22.375288 3 GAGTACT 10330 0.0 21.682425 12-13 GTACTTT 11210 0.0 19.937994 14-15 CATGGGG 5555 0.0 19.049032 4 AGTACTT 11245 0.0 18.905352 12-13 GTACCGT 305 4.629328E-9 18.669537 6 CATGGGA 6245 0.0 18.236055 4 TATAGCG 195 2.2946234E-4 17.033957 5 TTTGCGT 670 0.0 16.997637 3 GTACGGG 225 3.5717152E-5 16.871729 6 ATAGCGC 170 0.0018006876 16.747671 8 AGTACAT 8935 0.0 16.622713 2 ACTTTTT 13400 0.0 16.57283 16-17 CCTATAC 985 0.0 16.37928 3 AGAGTAC 20740 0.0 16.267733 10-11 GTATAGG 1050 0.0 15.877604 1 TAGCGTG 180 0.0026377775 15.817245 7 TACTTTT 13585 0.0 15.5616 14-15 >>END_MODULE