FastQCFastQC Report
Fri 27 May 2016
SRR938998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100349
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110690.3570243221005119No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT106940.3449289096163045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91780.2960311887468153No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58670.18923676011958654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56060.1808183530305782No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47500.153208558133294No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41080.13250121196033093No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC40530.13072721812931382No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35510.1145354926171215No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31200.10063383186860576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG112600.025.13571
TACATGG113350.024.132172
ACATGGG113550.022.5475923
GAGTACT95550.022.33792912-13
TTTGCGT5950.021.4751833
GTACTTT104300.020.30529614-15
CATGGGA58100.019.4675754
AGTACTT104150.019.35741612-13
CATGGGG55550.018.1461964
GTATAGG10250.018.0383071
AGTACAT84000.017.23982
GTATCAA296100.016.9235531
AGAGTAC197700.016.56519310-11
ATGGGAT25450.016.5497825
ACTTTTT130950.016.31878716-17
GTGTAGC16500.015.802791
TACCGTG2709.830184E-615.7742167
CGTATAG3102.107363E-615.2930221
CAACGCA323250.015.2845285
TACTTTT129550.015.23348214-15
Produced by FastQC (version 0.11.5)