##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938998_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3100349 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.794830194923218 33.0 31.0 34.0 30.0 34.0 2 31.858435937373503 33.0 31.0 34.0 30.0 34.0 3 31.90346506151404 34.0 31.0 34.0 30.0 34.0 4 35.44130870427813 37.0 35.0 37.0 33.0 37.0 5 35.48014271941643 37.0 35.0 37.0 33.0 37.0 6 35.56278986656018 37.0 35.0 37.0 33.0 37.0 7 35.47503555244909 37.0 35.0 37.0 33.0 37.0 8 35.46010207237959 37.0 35.0 37.0 33.0 37.0 9 37.0645643442077 39.0 37.0 39.0 33.0 39.0 10-11 36.956258311564284 39.0 37.0 39.0 33.0 39.0 12-13 36.8351796846097 39.0 37.0 39.0 33.0 39.0 14-15 38.04301273824334 40.0 38.0 41.0 33.0 41.0 16-17 37.96964228865847 40.0 38.0 41.0 33.0 41.0 18-19 37.92904492365214 40.0 38.0 41.0 33.0 41.0 20-21 37.80576283508728 40.0 38.0 41.0 32.0 41.0 22-23 37.88286931568027 40.0 38.0 41.0 32.0 41.0 24-25 37.81215356722743 40.0 38.0 41.0 32.0 41.0 26-27 37.72277846784346 40.0 38.0 41.0 32.0 41.0 28-29 37.60057787042685 40.0 37.0 41.0 32.0 41.0 30-31 37.42122564266152 40.0 37.0 41.0 31.5 41.0 32-33 37.23645983081259 40.0 37.0 41.0 31.0 41.0 34-35 36.92126047744947 40.0 36.0 41.0 30.5 41.0 36-37 36.65141021220514 39.5 36.0 41.0 30.0 41.0 38-39 36.441994917346406 39.0 35.5 41.0 30.0 41.0 40-41 36.29733378403528 39.0 35.0 41.0 30.0 41.0 42-43 36.09429938371454 39.0 35.0 41.0 29.0 41.0 44-45 36.02739610927673 39.0 35.0 41.0 28.5 41.0 46-47 35.80624729667531 39.0 35.0 41.0 27.0 41.0 48-49 35.612298970212706 39.0 35.0 41.0 27.0 41.0 50-51 34.44448753995115 38.0 34.0 40.0 24.5 40.5 52-53 34.588658567148414 38.0 34.0 39.5 25.0 40.5 54-55 35.093057749305 39.0 35.0 40.5 26.0 41.0 56-57 34.99010014678993 39.0 35.0 41.0 25.5 41.0 58-59 34.79163958638205 38.0 34.0 40.0 25.0 41.0 60-61 34.51216588842095 38.0 34.0 40.0 24.5 41.0 62-63 34.15810526492341 37.5 34.0 40.0 23.5 41.0 64-65 33.74738860044466 37.0 33.5 40.0 22.5 41.0 66-67 33.358301113842344 36.5 33.0 39.0 22.0 41.0 68-69 32.96014980894086 36.0 33.0 39.0 21.0 41.0 70-71 32.536048199734935 35.5 32.0 39.0 20.0 40.0 72-73 32.096200943829224 35.0 32.0 38.0 18.5 40.0 74-75 31.633249192268355 35.0 31.0 37.0 17.5 39.0 76-77 31.226137444526408 35.0 31.0 36.5 14.0 39.0 78-79 30.80128704865162 35.0 31.0 36.0 9.5 39.0 80-81 30.362910594903994 34.5 30.5 36.0 7.0 37.5 82-83 29.99223426136864 34.0 30.0 35.0 4.0 37.0 84-85 29.641284094145533 34.0 30.0 35.0 2.0 36.5 86-87 29.264326532271046 34.0 29.5 35.0 2.0 36.0 88-89 28.843030252400617 34.0 29.0 35.0 2.0 36.0 90-91 28.422107801412036 34.0 29.0 35.0 2.0 35.5 92-93 28.008094088762267 34.0 28.5 35.0 2.0 35.0 94-95 27.53518555491656 34.0 27.0 35.0 2.0 35.0 96-97 26.95222134669355 33.5 25.5 35.0 2.0 35.0 98-99 26.243495006529912 33.0 25.0 35.0 2.0 35.0 100-101 23.592818582682142 30.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25615744440183263 1101 2 0.2707886184195729 1101 3 0.3521703599710335 1101 4 0.16497999846624367 1101 5 0.010071878555017122 1101 6 0.11506367278070684 1101 7 0.19086317889663462 1101 8 0.27116275056378214 1101 9 0.21413659915757677 1101 10-11 0.3367348857934118 1101 12-13 0.5119932356805137 1101 14-15 0.15764728368746006 1101 16-17 0.40156296525308477 1101 18-19 0.3333192367523381 1101 20-21 0.5151779578612192 1101 22-23 0.4192547198738623 1101 24-25 0.47285349991853565 1101 26-27 0.30705718060472975 1101 28-29 0.34317311145424156 1101 30-31 0.4082100659062178 1101 32-33 0.32212281193280745 1101 34-35 0.2940991337935728 1101 36-37 0.2336668141703342 1101 38-39 0.22798252848774325 1101 40-41 0.19990738105754247 1101 42-43 0.19762167048412493 1101 44-45 0.42864050408837073 1101 46-47 0.45893113596682866 1101 48-49 0.5313774957870194 1101 50-51 0.3875571167777352 1101 52-53 0.33455697081939917 1101 54-55 0.28186203314511715 1101 56-57 0.2959523526517671 1101 58-59 0.2600710244849438 1101 60-61 0.28880577957432507 1101 62-63 0.2779331341159974 1101 64-65 0.23236017651999674 1101 66-67 0.04466045613090586 1101 68-69 0.06769747841430984 1101 70-71 0.07766819165681937 1101 72-73 -0.06795549644972709 1101 74-75 0.05454380773897327 1101 76-77 -0.00951873859311192 1101 78-79 -0.0835114357087221 1101 80-81 -0.029067756489528307 1101 82-83 -0.04103947713885603 1101 84-85 -0.1553376452779247 1101 86-87 -0.26266163227374406 1101 88-89 -0.20238681587102647 1101 90-91 -0.3221142585870638 1101 92-93 -0.23657712735065672 1101 94-95 -0.3252736064661619 1101 96-97 -0.5033817099943576 1101 98-99 -0.5299191475541534 1101 100-101 -0.2597891472460745 1102 1 0.277724217212711 1102 2 0.14093676314033843 1102 3 0.14551966325689847 1102 4 0.2520617205940425 1102 5 0.2907297320452358 1102 6 0.27955142272736566 1102 7 0.2630526816368999 1102 8 0.17117627646588573 1102 9 0.3430050752025835 1102 10-11 0.23164016483584504 1102 12-13 0.31242849045325016 1102 14-15 0.3592345240401329 1102 16-17 0.3290105825583325 1102 18-19 0.46481993854903436 1102 20-21 0.5372568333960572 1102 22-23 0.44201775991444237 1102 24-25 0.47056823781483814 1102 26-27 0.48488119784972383 1102 28-29 0.49119854867044666 1102 30-31 0.422842299231462 1102 32-33 0.33310382087989865 1102 34-35 0.36516709902573297 1102 36-37 0.3033657403699124 1102 38-39 0.36201120127013553 1102 40-41 0.30876201879117104 1102 42-43 0.16479780885209294 1102 44-45 0.27992155375518024 1102 46-47 0.1779421759666846 1102 48-49 0.2962964888871511 1102 50-51 0.17476225240773857 1102 52-53 0.13825270168732118 1102 54-55 0.19732135822508212 1102 56-57 0.15268796197353396 1102 58-59 0.11050933826528819 1102 60-61 -0.12181569835117756 1102 62-63 -0.01000403327609689 1102 64-65 -0.06859676626164912 1102 66-67 -0.06198270252580329 1102 68-69 -0.060006326340307226 1102 70-71 -0.23557258963621308 1102 72-73 -0.24621700503394095 1102 74-75 -0.3109013346693672 1102 76-77 -0.3337633678455312 1102 78-79 -0.4078609705798115 1102 80-81 -0.5673482486714256 1102 82-83 -0.2905872232774378 1102 84-85 -0.3533675023755265 1102 86-87 -0.33151953847120197 1102 88-89 -0.2752155742819653 1102 90-91 -0.37894316468239353 1102 92-93 -0.4923388598873615 1102 94-95 -0.37351409724221796 1102 96-97 -0.6537759551312732 1102 98-99 -0.663013491727181 1102 100-101 -0.17773248117148555 1103 1 -0.030715488693612514 1103 2 0.07797724177616061 1103 3 0.16605513980059072 1103 4 0.17079470505794347 1103 5 0.2286586865780933 1103 6 0.17666210997841603 1103 7 0.1720857916285894 1103 8 0.1428809003444229 1103 9 0.18807335471112907 1103 10-11 0.19263417101985425 1103 12-13 0.2578999804300466 1103 14-15 0.2906045158346444 1103 16-17 0.32891276869811037 1103 18-19 0.33884112068387395 1103 20-21 0.38757717378829426 1103 22-23 0.35517671287140473 1103 24-25 0.32373740688609587 1103 26-27 0.22152553035163436 1103 28-29 0.27125049869843565 1103 30-31 0.29819211963434356 1103 32-33 0.2871217968937714 1103 34-35 0.2815960236710211 1103 36-37 0.408053741326313 1103 38-39 0.40914797221724086 1103 40-41 0.40429491413577523 1103 42-43 0.3863122391135292 1103 44-45 0.4569415458213868 1103 46-47 0.3126006154008891 1103 48-49 0.23375403495476377 1103 50-51 0.08307534370611336 1103 52-53 0.18725065303036104 1103 54-55 0.28837247683525646 1103 56-57 0.36024082221383225 1103 58-59 0.15292387340491587 1103 60-61 0.24350820137875218 1103 62-63 0.18030641704805106 1103 64-65 0.1331171499336108 1103 66-67 0.14430401163492945 1103 68-69 0.10242445676832546 1103 70-71 0.02494240033787065 1103 72-73 0.08718833016872196 1103 74-75 0.05380036022746282 1103 76-77 0.10546705443864113 1103 78-79 0.03976785842525743 1103 80-81 0.057770622339262445 1103 82-83 -0.02150808643041202 1103 84-85 -0.054778467309258616 1103 86-87 0.09685762090561667 1103 88-89 0.01626565360802701 1103 90-91 -0.12567319299407487 1103 92-93 -0.04749229179532932 1103 94-95 -0.11055104371086699 1103 96-97 -0.06065036756885078 1103 98-99 -0.25000125956055186 1103 100-101 0.03926485068377161 1104 1 0.1998132627661242 1104 2 0.21768646310813722 1104 3 0.24741294655035517 1104 4 0.2526560579714996 1104 5 0.24174890437172536 1104 6 0.26476356368517173 1104 7 0.24185923783649343 1104 8 0.2442473786302486 1104 9 0.3215333792330526 1104 10-11 0.24036073822274062 1104 12-13 0.32237453035796193 1104 14-15 0.2783950956730621 1104 16-17 0.27857156311203113 1104 18-19 0.45168082484409666 1104 20-21 0.3191355943559202 1104 22-23 0.36369052633915544 1104 24-25 0.4238074112311949 1104 26-27 0.3336223931354283 1104 28-29 0.4609159775185958 1104 30-31 0.34681009648893735 1104 32-33 0.4006240525893361 1104 34-35 0.3739271252149905 1104 36-37 0.3012328015491761 1104 38-39 0.33073021968441196 1104 40-41 0.3795487921292633 1104 42-43 0.3775451365610678 1104 44-45 0.4082676517862538 1104 46-47 0.4886137309242713 1104 48-49 0.4849534264079125 1104 50-51 0.33486230286336394 1104 52-53 0.3809926050962176 1104 54-55 0.3924899171414751 1104 56-57 0.3759339002149815 1104 58-59 0.45954238016080495 1104 60-61 0.41337643274690095 1104 62-63 0.2689864007498528 1104 64-65 0.2859583374442636 1104 66-67 0.42486482072329324 1104 68-69 0.3986308813083568 1104 70-71 0.35612330122136626 1104 72-73 0.3086277701712845 1104 74-75 0.27565244453308324 1104 76-77 0.24182311078823204 1104 78-79 0.175081604535837 1104 80-81 0.1869446153810408 1104 82-83 0.04559317159536391 1104 84-85 0.136129259197304 1104 86-87 0.2050658781764234 1104 88-89 0.19583694433981336 1104 90-91 0.13347139425783539 1104 92-93 0.2687542904929501 1104 94-95 0.2515128168579217 1104 96-97 0.2446641435400707 1104 98-99 0.24005953448440565 1104 100-101 0.7754227538046621 1105 1 0.10606964856899026 1105 2 0.1928554812872605 1105 3 0.2515025595025193 1105 4 0.18476796563468412 1105 5 0.18019046763313185 1105 6 0.19888226525217334 1105 7 0.19966921883582955 1105 8 0.16891916874686785 1105 9 0.11686514295455197 1105 10-11 0.22477923864926197 1105 12-13 0.3307238569000148 1105 14-15 0.29752925249966466 1105 16-17 0.3108135345025076 1105 18-19 0.2625885212091106 1105 20-21 0.27423605494908543 1105 22-23 0.1889596649101648 1105 24-25 0.27245383189203665 1105 26-27 0.22742184532702225 1105 28-29 0.2182244287368178 1105 30-31 0.13631755814320456 1105 32-33 0.11124165004856224 1105 34-35 0.24663094935848306 1105 36-37 0.23245624968310352 1105 38-39 0.23613261593848733 1105 40-41 0.2224889408843822 1105 42-43 0.09495478219505316 1105 44-45 -0.021153910451744196 1105 46-47 0.27088620796630636 1105 48-49 0.27124888193741725 1105 50-51 0.10143558756009696 1105 52-53 0.24050735999103523 1105 54-55 0.21438341558582863 1105 56-57 0.16753267189156418 1105 58-59 0.1382810937726262 1105 60-61 0.20121797655217222 1105 62-63 0.1532216985721746 1105 64-65 0.186809121790958 1105 66-67 0.16512013475156806 1105 68-69 0.06151070478606613 1105 70-71 -0.038606446821631835 1105 72-73 0.14908028847607824 1105 74-75 0.22863331060118242 1105 76-77 0.13574773455654565 1105 78-79 0.22274481539484015 1105 80-81 0.12192416675396345 1105 82-83 0.04624309661050674 1105 84-85 0.06037588206703859 1105 86-87 0.1353297900187478 1105 88-89 0.2112800016752594 1105 90-91 0.1807180413071663 1105 92-93 0.1801216803169403 1105 94-95 0.34522825901555265 1105 96-97 0.2636045262187068 1105 98-99 0.3851975521737465 1105 100-101 0.35144216270916573 1106 1 0.11609860485346601 1106 2 0.05146835739940059 1106 3 0.14297464875405552 1106 4 0.08922451101106077 1106 5 0.08496119948620873 1106 6 0.07298844124640169 1106 7 0.12903923566290132 1106 8 0.08967080372836023 1106 9 0.08779365308095066 1106 10-11 0.038574503939848626 1106 12-13 0.13205995176905816 1106 14-15 0.1818805193993569 1106 16-17 0.2159899199414781 1106 18-19 0.22801666530107667 1106 20-21 0.2558679322049855 1106 22-23 0.21382875848937033 1106 24-25 0.2574040893888281 1106 26-27 0.27847305307466996 1106 28-29 0.21482476284353424 1106 30-31 0.1547116325353386 1106 32-33 0.2448461015585437 1106 34-35 0.1505555689510416 1106 36-37 0.19359750245602925 1106 38-39 0.13406766156413852 1106 40-41 0.17971570018755045 1106 42-43 0.28086613601340105 1106 44-45 0.40265924432244304 1106 46-47 0.14326723570690802 1106 48-49 0.06792834367035994 1106 50-51 0.015408641006665391 1106 52-53 -0.13769995106961375 1106 54-55 -0.1628091805030465 1106 56-57 -0.011522854121785997 1106 58-59 -0.019670817657555517 1106 60-61 0.0046653403571994545 1106 62-63 -0.04439036247008943 1106 64-65 -0.17499713504625447 1106 66-67 -0.0204914747652154 1106 68-69 -0.1054770282185089 1106 70-71 -0.058422605125578286 1106 72-73 -0.11583203202732761 1106 74-75 0.034483000205085546 1106 76-77 -0.09813952904966783 1106 78-79 0.016191055919581032 1106 80-81 -0.008950104981369833 1106 82-83 0.012942871456441907 1106 84-85 -0.0651522948379828 1106 86-87 0.12341051347765486 1106 88-89 0.130413559701541 1106 90-91 0.14803058491727938 1106 92-93 0.047282885425154575 1106 94-95 0.014887517750164392 1106 96-97 -0.07843730960862061 1106 98-99 -0.33457278957459735 1106 100-101 0.277295482223753 1107 1 0.039156873466804853 1107 2 0.010454557584896662 1107 3 -0.03597642231758158 1107 4 0.06255860919709022 1107 5 0.10489873749119738 1107 6 0.04680547151789938 1107 7 -0.0835395054100374 1107 8 0.039780727538506255 1107 9 -0.05254204559224007 1107 10-11 0.016290418345370483 1107 12-13 0.019819537362216977 1107 14-15 -0.04505072633947549 1107 16-17 0.1348348995098192 1107 18-19 0.06546543260129312 1107 20-21 0.010171160907056276 1107 22-23 -0.014685315531401955 1107 24-25 0.0960234981035839 1107 26-27 0.16826889444042337 1107 28-29 0.019425702778761433 1107 30-31 0.09705247005342699 1107 32-33 0.14978251641464624 1107 34-35 -0.030374997194975606 1107 36-37 -0.058095008334539955 1107 38-39 9.657985409177172E-4 1107 40-41 -0.21141881619594471 1107 42-43 -0.021724488062524472 1107 44-45 0.0645100445221587 1107 46-47 0.09002262967331376 1107 48-49 -0.025896761427425474 1107 50-51 0.03729611464995486 1107 52-53 -0.09751595427410109 1107 54-55 -0.026116202464997684 1107 56-57 -0.0035324833685805856 1107 58-59 0.04148727169069133 1107 60-61 -0.06665998849289423 1107 62-63 -0.14269281725237448 1107 64-65 0.04372070055809729 1107 66-67 -0.08910495843085897 1107 68-69 -0.00520549820016214 1107 70-71 -0.1318376599712181 1107 72-73 -0.14923211082324173 1107 74-75 -0.10511826439036653 1107 76-77 -0.0251317253585448 1107 78-79 6.318242938121443E-4 1107 80-81 0.0341848448674007 1107 82-83 0.19027146401926487 1107 84-85 0.1163608499525779 1107 86-87 0.2445457954450596 1107 88-89 0.22752908521676218 1107 90-91 0.3031022931477665 1107 92-93 0.19347113307597397 1107 94-95 0.2062878825980583 1107 96-97 0.24595370019875062 1107 98-99 0.1910296306357644 1107 100-101 0.25564815843308963 1108 1 0.09743273417871023 1108 2 0.09742741062610705 1108 3 0.04830051584199424 1108 4 0.12252235465707173 1108 5 0.08468948788277686 1108 6 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2316 82-83 -1.7127275339612211 2316 84-85 -1.84079288002901 2316 86-87 -1.8294240570973415 2316 88-89 -1.9020739968813096 2316 90-91 -2.0891061786124467 2316 92-93 -2.336394869235459 2316 94-95 -2.3987891100631735 2316 96-97 -2.7443628222554217 2316 98-99 -3.1424838071176886 2316 100-101 -3.5285185000270296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9536.0 3 2478.0 4 2459.0 5 2787.0 6 3442.0 7 4037.0 8 5191.0 9 6368.0 10 7715.0 11 10155.0 12 13146.0 13 18990.0 14 18057.0 15 16942.0 16 21064.0 17 27321.0 18 24111.0 19 17751.0 20 18020.0 21 19197.0 22 21185.0 23 23449.0 24 26891.0 25 30735.0 26 35793.0 27 42017.0 28 49401.0 29 59203.0 30 71197.0 31 86313.0 32 107923.0 33 138320.0 34 186327.0 35 266536.0 36 409500.0 37 628594.0 38 572595.0 39 95587.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55199891316829 19.24106153699352 14.209418884671306 22.99752066516688 2 15.281843118505789 20.731080172375112 40.1097537078759 23.877323001243195 3 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21.873925940873775 22-23 22.121368930322376 26.437698945442833 29.09660705938958 22.344325064845215 24-25 22.357976624793853 26.680366444844587 29.10490745625445 21.85674947410711 26-27 22.068774411101725 26.47139992213316 29.60782531085777 21.852000355907343 28-29 21.69927227745074 27.03073731216567 29.47624289195317 21.793747518430422 30-31 21.897150954003678 26.706516519842637 29.212998229457526 22.18333429669616 32-33 21.8235140266584 26.81071114899773 29.251088163876833 22.11468666046704 34-35 22.423437109447345 26.628810984493807 29.037853091979077 21.909898814079774 36-37 22.478463301881682 26.307766577977397 29.291547829427213 21.922222290713712 38-39 22.009623621924135 26.89983654634328 29.17201753842491 21.918522293307678 40-41 22.050099942753207 26.831642058867732 29.288633601225417 21.829624397153648 42-43 21.765270626393676 26.97425501723089 28.733265761200084 22.52720859517535 44-45 21.992283443467123 26.826712924048074 28.747011724066112 22.43399190841869 46-47 22.467606253321847 27.145173907112973 28.246961091822246 22.140258747742926 48-49 22.27523925800736 27.086177007935735 28.283902738785123 22.35468099527178 50-51 21.96990317562537 27.254544776204142 28.593637718844338 22.181914329326155 52-53 22.181424673124845 27.599647405356986 28.055687762325 22.163240159193172 54-55 22.08291830064246 27.219753788090756 28.12720543140935 22.57012247985743 56-57 22.28264297733446 27.464208742781466 27.874833851932053 22.37831442795202 58-59 22.38555465203669 27.072250251125098 28.08388510591034 22.458309990927876 60-61 22.142533368705784 27.287296530750126 28.502022598718067 22.06814750182602 62-63 22.04732493101914 27.10710509726086 28.56479756776083 22.28077240395917 64-65 21.911547807109265 27.48339122279116 28.316421845824923 22.28863912427465 66-67 22.002334578831466 27.35796031670311 27.87915163339389 22.76055347107154 68-69 21.79359616542293 27.29515631515836 28.345738268047192 22.56550925137152 70-71 22.26105724579222 27.114939410440314 28.048429461239362 22.5755738825281 72-73 22.138315862260747 26.86469649233559 28.241323236015475 22.75566440938819 74-75 21.79566405749977 27.799284238035067 27.77212558330674 22.632926121158427 76-77 22.024490526867996 27.1896139844091 27.93092535286502 22.85497013585788 78-79 22.061132606424504 27.16425767433503 28.11028571558311 22.664324003657356 80-81 22.198257793218797 27.289843083982007 27.85712976808577 22.654769354713427 82-83 22.07191981891916 27.17763140395391 28.122463768586055 22.627985008540875 84-85 21.998274460671638 27.18488428845996 27.910785172300084 22.906056078568323 86-87 21.877321861475828 27.139567220023547 28.217438064636568 22.765672853864054 88-89 22.120664095848177 27.001720890087373 27.885280915152123 22.99233409891233 90-91 21.99301074248329 26.834371858285593 28.142493118545314 23.030124280685804 92-93 22.177145108178582 27.02345675647261 28.025888486953544 22.773509648395265 94-95 22.05104634063083 26.80877140153703 28.07344307914756 23.066739178684582 96-97 21.869088433625947 26.558933794350338 28.271280341038363 23.300697430985355 98-99 22.010684834693002 26.83060701529747 27.848713280209854 23.30999486979967 100-101 22.165993364028687 26.125142642563485 28.42103299574931 23.287830997658514 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3575.0 1 2085.5 2 696.0 3 801.0 4 725.5 5 739.5 6 1129.5 7 2111.5 8 3429.5 9 4170.5 10 3950.0 11 3659.5 12 3843.0 13 4168.0 14 4497.0 15 4824.5 16 5132.5 17 5314.0 18 5479.0 19 5838.5 20 6365.0 21 7237.0 22 8578.5 23 10239.5 24 11933.5 25 14596.0 26 19327.0 27 24786.0 28 28495.0 29 31598.0 30 35873.0 31 41969.0 32 49111.5 33 56110.5 34 63676.5 35 70597.5 36 79007.5 37 87249.0 38 91682.0 39 93981.5 40 94256.5 41 94335.5 42 93509.5 43 94589.0 44 98129.5 45 100233.0 46 102958.0 47 112641.5 48 137071.5 49 136304.5 50 111611.0 51 101933.0 52 100119.5 53 99938.0 54 97434.5 55 92333.5 56 86012.0 57 79027.5 58 72645.5 59 65498.5 60 55262.0 61 44678.0 62 35396.0 63 26852.5 64 19782.0 65 14369.0 66 10256.0 67 7938.5 68 5865.5 69 4041.0 70 2915.5 71 1849.5 72 1173.0 73 827.0 74 585.5 75 388.0 76 247.5 77 152.0 78 88.5 79 53.0 80 39.0 81 33.0 82 31.5 83 32.0 84 25.0 85 15.0 86 9.5 87 11.5 88 12.5 89 8.5 90 5.5 91 6.5 92 7.0 93 4.0 94 3.0 95 2.0 96 2.5 97 2.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2836454863629869 2 0.08663540782021636 3 0.090247904348833 4 0.1057300323286185 5 0.10776206162596534 6 0.08205527829286316 7 0.09257022354580081 8 0.08889321815060175 9 0.09440872624340034 10-11 0.08695795215312857 12-13 0.09177998993016592 14-15 0.1010047578514548 16-17 0.08924801691680517 18-19 0.10023065145246551 20-21 0.10197239085019139 22-23 0.1145516198337671 24-25 0.12735662985038135 26-27 0.13022727441329993 28-29 0.12161534072454425 30-31 0.11476127365016003 32-33 0.10931027442394388 34-35 0.3798604608706955 36-37 0.5822570297730997 38-39 0.5650331623955883 40-41 0.5268277861621385 42-43 0.5555503590079698 44-45 0.5769027938467572 46-47 0.5657266327113496 48-49 0.5875467568328598 50-51 0.5794186396434724 52-53 0.5823860475062647 54-55 0.5728548624687092 56-57 0.5444387067391445 58-59 0.5395037784455878 60-61 0.4865742534146962 62-63 0.5621463906160241 64-65 0.595723255672184 66-67 0.6080283219727843 68-69 0.6100603512701313 70-71 0.606222073708476 72-73 0.5980133204358606 74-75 0.5953684569059806 76-77 0.579725056759739 78-79 0.556324465406959 80-81 0.5507605756642237 82-83 0.5653234522952093 84-85 0.5752416905322595 86-87 0.5663394669438828 88-89 0.578402624994799 90-91 0.5913366527445781 92-93 0.5997066781836496 94-95 0.5986745363183307 96-97 0.5956426195889559 98-99 0.5998679503501058 100-101 0.5775640097292273 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3100349.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.94637735250978 #Duplication Level Percentage of deduplicated Percentage of total 1 71.11977995647277 31.25456687194614 2 14.285022245474654 12.555499561772516 3 5.450971719054292 7.186513803102565 4 2.7344461639225375 4.806760118794506 5 1.559444509464224 3.4265968436607146 6 1.027655781270595 2.7097049251323497 7 0.7073853371150426 2.1760916070943 8 0.5019218917219214 1.7646119084077687 9 0.3720386955568763 1.471477761421019 >10 1.980753852344551 15.884745203395342 >50 0.16324744804216162 4.942813083392138 >100 0.09026733160294136 7.215394610817009 >500 0.00456285638927419 1.3212070525501598 >1k 0.0021342392787038534 1.9122588965924527 >5k 2.2078337365901934E-4 0.6678966693760908 >10k+ 1.4718891577267953E-4 0.7038610825447612 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11069 0.3570243221005119 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10694 0.3449289096163045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9178 0.2960311887468153 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5867 0.18923676011958654 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5606 0.1808183530305782 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4750 0.153208558133294 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4108 0.13250121196033093 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4053 0.13072721812931382 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3551 0.1145354926171215 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3120 0.10063383186860576 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.22544332912198E-5 0.0 2 0.0 0.0 0.0 3.22544332912198E-5 0.0 3 0.0 0.0 0.0 3.22544332912198E-5 0.0 4 0.0 0.0 0.0 3.22544332912198E-5 0.0 5 0.0 0.0 0.0 3.22544332912198E-5 0.0 6 0.0 0.0 0.0 6.45088665824396E-5 3.22544332912198E-5 7 0.0 0.0 0.0 6.45088665824396E-5 3.22544332912198E-5 8 0.0 0.0 0.0 6.45088665824396E-5 6.45088665824396E-5 9 0.0 0.0 0.0 3.2254433291219794E-4 6.45088665824396E-5 10-11 3.22544332912198E-5 0.0 0.0 4.838164993682969E-4 6.45088665824396E-5 12-13 6.45088665824396E-5 0.0 0.0 5.483253659507365E-4 8.063608322804948E-5 14-15 6.45088665824396E-5 0.0 0.0 6.28961449178786E-4 1.7739938310170887E-4 16-17 9.676329987365939E-5 0.0 0.0 7.095975324068355E-4 2.580354663297584E-4 18-19 1.290177331648792E-4 0.0 0.0 9.676329987365938E-4 3.5479876620341774E-4 20-21 1.6127216645609897E-4 0.0 0.0 0.001241795681711962 4.031804161402474E-4 22-23 1.9352659974731877E-4 0.0 0.0 0.0014514494981048907 4.6768928272268703E-4 24-25 1.9352659974731877E-4 0.0 0.0 0.0017739938310170887 6.128342325331761E-4 26-27 1.9352659974731877E-4 0.0 0.0 0.0022900647636766055 6.128342325331761E-4 28-29 1.9352659974731877E-4 0.0 0.0 0.005112327676658338 6.128342325331761E-4 30-31 2.2578103303853855E-4 0.0 0.0 0.013466225899084264 6.450886658243959E-4 32-33 2.2578103303853855E-4 0.0 0.0 0.028303265213045367 7.741063989892751E-4 34-35 3.0641711626658806E-4 0.0 0.0 0.05444548339557902 9.031241321541542E-4 36-37 4.031804161402474E-4 0.0 0.0 0.09529572315890888 9.031241321541542E-4 38-39 4.1930763278585735E-4 0.0 0.0 0.15370850184930793 9.031241321541542E-4 40-41 4.1930763278585735E-4 0.0 0.0 0.217701297499088 9.031241321541542E-4 42-43 4.1930763278585735E-4 0.0 0.0 0.28606456885982834 9.192513487997641E-4 44-45 4.1930763278585735E-4 0.0 0.0 0.37194199749770107 9.515057820909839E-4 46-47 4.3543484943146723E-4 0.0 0.0 0.476123817028341 9.676329987365938E-4 48-49 4.838164993682969E-4 0.0 0.0 0.5886111531314699 0.0010321418653190335 50-51 5.160709326595168E-4 0.0 0.0 0.7078235385758185 0.0010643962986102533 52-53 5.160709326595168E-4 0.0 0.0 0.828019684235549 0.001096650731901473 54-55 5.160709326595168E-4 0.0 0.0 0.9646333364405104 0.0011289051651926928 56-57 5.160709326595168E-4 0.0 0.0 1.1322112446050427 0.0011289051651926928 58-59 5.160709326595168E-4 0.0 0.0 1.315593825082273 0.0011289051651926928 60-61 5.160709326595168E-4 0.0 0.0 1.5180710300679054 0.0011289051651926928 62-63 5.160709326595168E-4 0.0 0.0 1.7269991217117815 0.0011611595984839125 64-65 5.160709326595168E-4 0.0 0.0 1.945716433859543 0.0011611595984839125 66-67 5.160709326595168E-4 0.0 0.0 2.1960269634160543 0.0011611595984839125 68-69 5.160709326595168E-4 0.0 0.0 2.4901873950319784 0.0011611595984839125 70-71 5.160709326595168E-4 0.0 0.0 2.8078613085172024 0.0012095412484207425 72-73 5.160709326595168E-4 0.0 0.0 3.131663564327758 0.001241795681711962 74-75 5.160709326595168E-4 0.0 0.0 3.470867312034871 0.001257922898357572 76-77 5.160709326595168E-4 0.0 0.0 3.8391161769207276 0.0012901773316487918 78-79 5.160709326595168E-4 0.0 0.0 4.260439711787286 0.0012901773316487918 80-81 5.160709326595168E-4 0.0 0.0 4.728709574309216 0.0013385589815856215 82-83 5.160709326595168E-4 0.0 0.0 5.203269051322932 0.0013869406315224512 84-85 5.160709326595168E-4 0.0 0.0 5.693504182916182 0.001403067848168061 86-87 5.160709326595168E-4 1.61272166456099E-5 0.0 6.21783225049825 0.001419195064813671 88-89 5.160709326595168E-4 3.22544332912198E-5 0.0 6.779204534715285 0.001419195064813671 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11260 0.0 25.1357 1 TACATGG 11335 0.0 24.13217 2 ACATGGG 11355 0.0 22.547592 3 GAGTACT 9555 0.0 22.337929 12-13 TTTGCGT 595 0.0 21.475183 3 GTACTTT 10430 0.0 20.305296 14-15 CATGGGA 5810 0.0 19.467575 4 AGTACTT 10415 0.0 19.357416 12-13 CATGGGG 5555 0.0 18.146196 4 GTATAGG 1025 0.0 18.038307 1 AGTACAT 8400 0.0 17.2398 2 GTATCAA 29610 0.0 16.923553 1 AGAGTAC 19770 0.0 16.565193 10-11 ATGGGAT 2545 0.0 16.549782 5 ACTTTTT 13095 0.0 16.318787 16-17 GTGTAGC 1650 0.0 15.80279 1 TACCGTG 270 9.830184E-6 15.774216 7 CGTATAG 310 2.107363E-6 15.293022 1 CAACGCA 32325 0.0 15.284528 5 TACTTTT 12955 0.0 15.233482 14-15 >>END_MODULE