FastQCFastQC Report
Fri 27 May 2016
SRR938998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100349
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT117790.37992496973727796No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112230.36199150482735976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95390.30767503916494565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60680.1957199012111217No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55780.179915228898424No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44700.14417731681175247No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41600.13417844249147434No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39540.12753402923348306No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35320.11392265838458832No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32440.104633381596717No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG116750.023.9587021
TACATGG118000.023.243582
ACATGGG117500.022.4136333
GAGTACT106250.021.23732212-13
GTACTTT112700.020.1692514-15
CATGGGG57950.019.8980264
AGTACTT114300.018.68290512-13
GTATACG1559.4249693E-418.4459741
CATGGGA62800.017.8323884
ACTTTTT135250.016.73548316-17
AGTACAT89150.015.9682392
AGAGTAC212650.015.93349310-11
GTATCAA310250.015.4514151
GTATAGG10800.015.4428111
TACTTTT135600.015.41573914-15
GTGTAGC16400.015.3997651
CGTATAG2801.2992017E-515.3167471
GGTATCA262250.014.5000491
TTTGCGT6950.014.33813
GTGTAGG12650.014.3144651
Produced by FastQC (version 0.11.5)