##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938998_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3100349 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.156762029048984 33.0 31.0 34.0 30.0 34.0 2 32.38391935875606 34.0 31.0 34.0 30.0 34.0 3 32.479517951043576 34.0 31.0 34.0 30.0 34.0 4 36.05499832438219 37.0 35.0 37.0 35.0 37.0 5 35.96837581833529 37.0 35.0 37.0 35.0 37.0 6 36.012263780625986 37.0 35.0 37.0 35.0 37.0 7 35.95643748494121 37.0 35.0 37.0 35.0 37.0 8 35.925456134132 37.0 35.0 37.0 35.0 37.0 9 37.59871646708161 39.0 37.0 39.0 35.0 39.0 10-11 37.52265986829225 39.0 37.0 39.0 35.0 39.0 12-13 37.45353829520483 39.0 37.0 39.0 34.0 39.0 14-15 38.70767645836001 40.0 38.0 41.0 34.0 41.0 16-17 38.69922353902738 40.0 38.0 41.0 34.0 41.0 18-19 38.80725557026 40.0 38.0 41.0 34.0 41.0 20-21 38.80767858715261 40.0 38.0 41.0 34.0 41.0 22-23 38.742472702266745 40.0 38.0 41.0 34.0 41.0 24-25 38.679534304041255 40.0 38.0 41.0 34.0 41.0 26-27 38.51898092763105 40.0 38.0 41.0 34.0 41.0 28-29 38.34516565715666 40.0 38.0 41.0 34.0 41.0 30-31 38.182016766499515 40.0 38.0 41.0 33.5 41.0 32-33 38.02679182246902 40.0 37.0 41.0 33.0 41.0 34-35 37.99884690400984 40.0 37.0 41.0 33.0 41.0 36-37 37.89068730649356 40.0 37.0 41.0 33.0 41.0 38-39 37.76198211878727 40.0 37.0 41.0 33.0 41.0 40-41 37.61088380695206 40.0 37.0 41.0 33.0 41.0 42-43 37.44260194578094 40.0 37.0 41.0 32.0 41.0 44-45 37.19845927023054 40.0 36.5 41.0 31.0 41.0 46-47 36.89036363325548 40.0 36.0 41.0 31.0 41.0 48-49 36.59875484985723 39.5 36.0 41.0 30.0 41.0 50-51 36.33230662096429 39.0 35.5 41.0 30.0 41.0 52-53 36.10345335315476 39.0 35.0 41.0 29.5 41.0 54-55 35.85826998831422 39.0 35.0 40.0 28.5 41.0 56-57 35.57724710992214 38.5 35.0 40.0 28.0 41.0 58-59 35.27434975868846 38.0 34.5 40.0 28.0 41.0 60-61 34.86055731145106 38.0 34.0 40.0 26.5 41.0 62-63 34.544158093169514 37.0 34.0 40.0 26.0 41.0 64-65 34.28461215172872 37.0 34.0 39.5 26.0 41.0 66-67 33.82429752263374 36.0 33.0 39.0 26.0 40.5 68-69 33.40039734236372 36.0 33.0 39.0 25.0 40.0 70-71 32.935982207164415 35.0 32.0 38.0 24.5 40.0 72-73 32.42022107833667 35.0 32.0 37.5 23.0 39.5 74-75 31.954083878943948 35.0 31.0 37.0 22.0 39.0 76-77 29.97239407563471 33.0 29.0 35.0 19.5 37.5 78-79 30.961571745632508 34.0 30.5 36.0 20.0 38.0 80-81 30.96469574876893 34.0 31.0 35.5 20.0 37.0 82-83 30.645838742670584 34.0 31.0 35.0 18.5 37.0 84-85 30.244482798549456 34.0 30.5 35.0 16.5 36.5 86-87 29.683760924979737 34.0 30.0 35.0 8.5 36.0 88-89 29.102966956300726 34.0 29.0 35.0 3.5 35.5 90-91 28.645565708892775 34.0 29.0 35.0 2.0 35.0 92-93 28.296141821453006 33.0 29.0 35.0 2.0 35.0 94-95 27.90149334800695 33.0 28.0 35.0 2.0 35.0 96-97 27.271684897409937 33.0 27.0 35.0 2.0 35.0 98-99 26.370677301168353 33.0 25.5 34.5 2.0 35.0 100-101 21.986825515450036 28.5 9.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09572976478659001 1101 2 0.10512954614515024 1101 3 0.106101389115409 1101 4 0.0639781309607983 1101 5 0.1146307384188816 1101 6 0.11476270392746812 1101 7 0.15048271241490596 1101 8 0.1506367131576738 1101 9 0.2580702441296481 1101 10-11 0.22106523647956777 1101 12-13 0.22775645969272063 1101 14-15 0.28497202879482586 1101 16-17 0.2811078493181043 1101 18-19 0.3521747489327609 1101 20-21 0.31086765860389676 1101 22-23 0.25567791587432254 1101 24-25 0.3525220625145735 1101 26-27 0.30616069548652547 1101 28-29 0.3542421399074911 1101 30-31 0.2724512638351726 1101 32-33 0.29547786917100893 1101 34-35 0.3336696699932844 1101 36-37 0.3478674448379806 1101 38-39 0.39095165283003297 1101 40-41 0.40288303246311585 1101 42-43 0.22707379761516222 1101 44-45 0.27565560926630184 1101 46-47 0.30437147379912943 1101 48-49 0.1236505587278387 1101 50-51 0.2118617959771072 1101 52-53 0.2568735382540126 1101 54-55 0.27635586490468 1101 56-57 0.04795102452060007 1101 58-59 0.13742481400536377 1101 60-61 0.3089084455834694 1101 62-63 0.1836777482408536 1101 64-65 0.0887752776989359 1101 66-67 0.1378793633454265 1101 68-69 0.11605639221572517 1101 70-71 0.14106219705775658 1101 72-73 0.07775435444541046 1101 74-75 0.1350034874559256 1101 76-77 -0.05429147753668673 1101 78-79 0.21441694257016408 1101 80-81 0.26561437937673205 1101 82-83 0.36535860558393196 1101 84-85 0.22372962266580032 1101 86-87 0.29716611897981693 1101 88-89 0.5307729797204566 1101 90-91 0.491742401627306 1101 92-93 0.508633041496136 1101 94-95 0.35774280065315267 1101 96-97 0.2518247998818097 1101 98-99 0.3522178265566609 1101 100-101 1.0096117445646264 1102 1 0.10957138960836232 1102 2 0.09274701469978908 1102 3 0.14588493681397807 1102 4 0.07437212995706943 1102 5 0.06748924198075912 1102 6 0.061729452337289104 1102 7 0.005865731526426998 1102 8 0.11448350338299917 1102 9 0.1545839576548218 1102 10-11 0.2004621989856048 1102 12-13 0.16337097645294563 1102 14-15 0.23600512725712264 1102 16-17 0.2552572233220758 1102 18-19 0.16967021337708843 1102 20-21 0.23202685563155967 1102 22-23 0.24915394609423913 1102 24-25 0.2284422054938915 1102 26-27 0.25152149448747707 1102 28-29 0.18302622994384166 1102 30-31 0.22421769576491357 1102 32-33 0.27047666509354684 1102 34-35 0.324823990359306 1102 36-37 0.3364117276119387 1102 38-39 0.22509409466126584 1102 40-41 0.216915960638687 1102 42-43 0.2034808020680572 1102 44-45 0.2406631656659428 1102 46-47 0.35060538575627476 1102 48-49 0.21479117568648576 1102 50-51 0.19782485618228662 1102 52-53 0.32574422483140353 1102 54-55 0.32895807451403414 1102 56-57 0.23424476522302484 1102 58-59 0.22439630535617994 1102 60-61 0.09570792248725013 1102 62-63 0.13070123917086107 1102 64-65 0.08385991990687813 1102 66-67 0.18978989405898972 1102 68-69 0.1715002246351247 1102 70-71 0.11259910756420766 1102 72-73 0.10699142611482415 1102 74-75 0.20625268557584064 1102 76-77 -0.004806615600884356 1102 78-79 0.12453696808832504 1102 80-81 0.3208249698622332 1102 82-83 0.2859469456409691 1102 84-85 0.26027145066011315 1102 86-87 0.3041599452279513 1102 88-89 0.47243604796070215 1102 90-91 0.5035841336622617 1102 92-93 0.421334881267331 1102 94-95 0.48309501549891465 1102 96-97 0.474201044090254 1102 98-99 0.6421828142324699 1102 100-101 1.1144111559097958 1103 1 0.03142626644441293 1103 2 0.03041241522037552 1103 3 0.0505463106639894 1103 4 0.07962690789243254 1103 5 0.04510617029950481 1103 6 0.02445965454027288 1103 7 0.10265254621806719 1103 8 0.08008291215255525 1103 9 0.0660977358238597 1103 10-11 0.12797261887877198 1103 12-13 0.04107783918205854 1103 14-15 0.08807932450419997 1103 16-17 0.06199062077783424 1103 18-19 0.014731700256447766 1103 20-21 0.0979009583252477 1103 22-23 0.10381477653391613 1103 24-25 0.08552528749994792 1103 26-27 0.16443727131971997 1103 28-29 0.1946171723803829 1103 30-31 0.20830664116848396 1103 32-33 0.1092454602984958 1103 34-35 0.13102756273884353 1103 36-37 0.016662294117210763 1103 38-39 0.05506915212690444 1103 40-41 0.05256693904898668 1103 42-43 0.042820178124316044 1103 44-45 0.08482123286406562 1103 46-47 0.23151021830438623 1103 48-49 -0.022588119292947795 1103 50-51 -0.11334412812946226 1103 52-53 -0.10959402093135395 1103 54-55 -0.026737952613011373 1103 56-57 -0.1217433449588583 1103 58-59 -0.14043037391760294 1103 60-61 -0.008734185292759378 1103 62-63 -0.043131651283047745 1103 64-65 -0.028901800095518126 1103 66-67 0.04178665533339654 1103 68-69 -0.05018809693336834 1103 70-71 -0.030111314502597963 1103 72-73 -0.17369359695294406 1103 74-75 -0.11161247258374374 1103 76-77 -0.13669773790624618 1103 78-79 0.09482464951944891 1103 80-81 0.08021374191790187 1103 82-83 0.09249422505712701 1103 84-85 0.04134637064294111 1103 86-87 -0.07933559609025309 1103 88-89 0.18989120302284235 1103 90-91 0.28298942539209904 1103 92-93 0.26319296422435556 1103 94-95 0.1375634786801463 1103 96-97 -0.14532341752595457 1103 98-99 -0.06790641374446693 1103 100-101 0.5845167948788372 1104 1 0.06401624529267025 1104 2 0.03774217218646925 1104 3 0.012292078858763489 1104 4 0.060741244591064 1104 5 0.07327723419062693 1104 6 0.07210750621269568 1104 7 -0.022236464883967244 1104 8 0.06069521869019212 1104 9 0.12551210160641801 1104 10-11 0.07864287870532394 1104 12-13 0.12260969471585526 1104 14-15 0.10104004067326144 1104 16-17 0.04673041988647242 1104 18-19 0.07217765300706702 1104 20-21 0.09730126259867689 1104 22-23 0.0715576769178341 1104 24-25 0.02778808728658788 1104 26-27 0.03220625861351323 1104 28-29 0.06261332686434429 1104 30-31 0.027245877676811858 1104 32-33 0.05740416993287312 1104 34-35 0.040770003190836235 1104 36-37 -0.020530444182689678 1104 38-39 0.2197063574946796 1104 40-41 0.1330784663845037 1104 42-43 0.06932987786012745 1104 44-45 0.07001116819138531 1104 46-47 -0.009993285644569028 1104 48-49 -0.11026491728977561 1104 50-51 -0.19736339442608397 1104 52-53 -0.2362671924162285 1104 54-55 -0.15281941653996256 1104 56-57 -0.2236679455096109 1104 58-59 -0.22971314365940287 1104 60-61 -0.09496926461818589 1104 62-63 0.006396218795977404 1104 64-65 0.029203847108242087 1104 66-67 0.10739879044609779 1104 68-69 0.0918420663914361 1104 70-71 -0.18891517429239002 1104 72-73 -0.0899000401514698 1104 74-75 -0.07930820195718269 1104 76-77 -0.24186705093073968 1104 78-79 -0.12091112145310845 1104 80-81 -0.0549472377557656 1104 82-83 -0.12955348191865212 1104 84-85 -0.2217303197410061 1104 86-87 -0.24249120025119453 1104 88-89 -0.11997224943799267 1104 90-91 -0.15484524680494616 1104 92-93 -0.05965575198948514 1104 94-95 0.015785480265652296 1104 96-97 0.04035165036181709 1104 98-99 0.12999253463937066 1104 100-101 0.5927100320602747 1105 1 -0.031815811114384474 1105 2 -0.04551188394055572 1105 3 0.008873758935727949 1105 4 -0.022396749481004008 1105 5 0.037492221153321736 1105 6 -0.005916334867762885 1105 7 0.025788933812243897 1105 8 0.021047993354926575 1105 9 -0.02317227539507627 1105 10-11 -0.039575658721602736 1105 12-13 -0.01160460059796975 1105 14-15 0.0957443157943132 1105 16-17 0.03467233712589746 1105 18-19 0.032579742798837685 1105 20-21 0.06854621370247571 1105 22-23 -0.015112787257287152 1105 24-25 0.06691505771269135 1105 26-27 -0.07866606806517495 1105 28-29 -0.010645278400396307 1105 30-31 0.03848753360792756 1105 32-33 0.02888416919062564 1105 34-35 0.09511628535321393 1105 36-37 0.041262253467223786 1105 38-39 0.1357504183395335 1105 40-41 0.2641678021148053 1105 42-43 0.1672865896376763 1105 44-45 0.1667199317200101 1105 46-47 0.17398778904282608 1105 48-49 0.10464455824357088 1105 50-51 0.12849820044873894 1105 52-53 0.27390685005775595 1105 54-55 0.31881983065365915 1105 56-57 0.2247084509322761 1105 58-59 0.12267436639903195 1105 60-61 0.027033266929151978 1105 62-63 0.043419002159737374 1105 64-65 0.01425579668647714 1105 66-67 0.22490852835615271 1105 68-69 0.2652429938757166 1105 70-71 0.2932875755460813 1105 72-73 0.24534368735237422 1105 74-75 0.3349953779161794 1105 76-77 0.12147595236611863 1105 78-79 0.31569560418553166 1105 80-81 0.2742934273742783 1105 82-83 0.35892072379571616 1105 84-85 0.28760806469093225 1105 86-87 0.295241282335283 1105 88-89 0.30344894954376755 1105 90-91 0.34726945556954547 1105 92-93 0.4876707439542507 1105 94-95 0.4258344073945395 1105 96-97 0.2289442798590109 1105 98-99 0.4397222911171106 1105 100-101 1.0051113526035387 1106 1 -0.06616417297885135 1106 2 -0.186420036686755 1106 3 -0.12115783124735202 1106 4 -0.09459069295289169 1106 5 -0.08922497283717945 1106 6 -0.06379393273009981 1106 7 -0.08029531663610356 1106 8 -0.11793663873204707 1106 9 -0.10171302916594982 1106 10-11 -0.05370658008256868 1106 12-13 -0.038847412295801576 1106 14-15 -0.16237326564489507 1106 16-17 -0.1380938152849751 1106 18-19 -0.2546582991078097 1106 20-21 -0.1698263689623758 1106 22-23 -0.07414111107409127 1106 24-25 -0.0468300196359408 1106 26-27 -0.04471732838013054 1106 28-29 0.013229850458664316 1106 30-31 -0.036999170460838116 1106 32-33 0.010877045490900628 1106 34-35 -0.044134005288647415 1106 36-37 -0.27696847494407706 1106 38-39 -0.10480315410678998 1106 40-41 -0.029915664804036624 1106 42-43 0.015385285581196229 1106 44-45 0.047565512774092156 1106 46-47 -0.05687353453549093 1106 48-49 -0.15108602491596201 1106 50-51 -0.15883534794942022 1106 52-53 0.0245409500806133 1106 54-55 0.015716644355691756 1106 56-57 -0.09909042766879139 1106 58-59 -0.11006930933928061 1106 60-61 -0.0021810953436798286 1106 62-63 -0.18994878581363395 1106 64-65 -0.2180894742205055 1106 66-67 -0.034540997901771675 1106 68-69 -0.19265823160648665 1106 70-71 -0.14952213025961925 1106 72-73 -0.1536964336741633 1106 74-75 0.0213348509261877 1106 76-77 -0.14333178686906223 1106 78-79 0.0730504617087675 1106 80-81 0.1489080675344816 1106 82-83 0.30751017702381844 1106 84-85 0.1573631619210758 1106 86-87 0.22672218076031214 1106 88-89 0.2652396021917447 1106 90-91 0.42056592907811563 1106 92-93 0.22905012108149592 1106 94-95 0.3858383803760539 1106 96-97 0.3680768197396098 1106 98-99 0.37527831762652397 1106 100-101 0.8101791727259702 1107 1 -0.1950264231550065 1107 2 -0.14036544814094043 1107 3 -0.13808118423286686 1107 4 -0.11711213149602173 1107 5 -0.14374825755630383 1107 6 -0.09117759688050597 1107 7 -0.11238293453382653 1107 8 -0.09702972671584575 1107 9 -0.09633277307045773 1107 10-11 -0.029149434179387868 1107 12-13 -0.11381710701866865 1107 14-15 -0.012403494144265892 1107 16-17 -0.022502984128465187 1107 18-19 -0.1675471345691335 1107 20-21 -0.15032443504038895 1107 22-23 -0.15370951917726217 1107 24-25 -0.22305016076662554 1107 26-27 -0.19026497533933195 1107 28-29 -0.18381516017176835 1107 30-31 -0.19538285172171044 1107 32-33 -0.09248462468413976 1107 34-35 -0.2375175944162109 1107 36-37 -0.16863791292212937 1107 38-39 -0.0977264934916704 1107 40-41 -0.17627457843827443 1107 42-43 -0.18911429674781743 1107 44-45 -0.2899964468750298 1107 46-47 -0.29639479670915847 1107 48-49 -0.13183471552617476 1107 50-51 -0.1083625285912504 1107 52-53 -0.10270397666463538 1107 54-55 -0.25846324855908165 1107 56-57 -0.10740024872836074 1107 58-59 -0.13296981063221835 1107 60-61 -0.20233428890347227 1107 62-63 -0.14216763005832433 1107 64-65 -0.17256481671510926 1107 66-67 -0.12757293393843838 1107 68-69 -0.08453315976444031 1107 70-71 -0.18746394237260233 1107 72-73 -0.09686745810002151 1107 74-75 -0.13075075100703515 1107 76-77 -0.4744138135912337 1107 78-79 -0.178057112263307 1107 80-81 0.029007810614171348 1107 82-83 0.04090111926676698 1107 84-85 -0.025378975378608004 1107 86-87 0.024628264892935903 1107 88-89 0.026044810005000585 1107 90-91 0.19281157095331736 1107 92-93 0.24770541571942672 1107 94-95 0.1757127982909772 1107 96-97 0.048623003534149944 1107 98-99 -0.116864411344487 1107 100-101 0.46910397264313985 1108 1 -0.06254605465390739 1108 2 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Count 2 2.0 3 5.0 4 29.0 5 56.0 6 156.0 7 387.0 8 864.0 9 1630.0 10 2780.0 11 4239.0 12 6238.0 13 8714.0 14 10476.0 15 14077.0 16 18274.0 17 25596.0 18 25000.0 19 17638.0 20 16220.0 21 16984.0 22 18660.0 23 20637.0 24 24072.0 25 28454.0 26 34374.0 27 41970.0 28 50700.0 29 62173.0 30 75980.0 31 93193.0 32 114813.0 33 145848.0 34 197159.0 35 283276.0 36 448318.0 37 710368.0 38 540099.0 39 40889.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.38500265880447 20.008264639455103 14.743184101320853 22.863548600419577 2 15.582761811654109 21.327792451753012 39.40491860755031 23.684527129042568 3 19.45593867013036 25.120752534634004 30.431251449433596 24.99205734580204 4 12.97008820619872 17.203837374437523 37.371921677204725 32.454152742159025 5 13.18376885078548 37.95218903763626 34.6922509986824 14.171791112895866 6 31.120206144534052 36.98035285704932 17.285344327364434 14.614096671052195 7 27.609375589651357 33.32876395528374 21.274411364656043 17.787449090408856 8 25.55380055600192 35.23877473148991 19.878020184179267 19.329404528328908 9 25.818448181156377 17.303761608773723 21.084852060203545 35.79293814986635 10-11 24.720604035223133 26.62938914296423 27.52646556887628 21.123541252936363 12-13 25.903761157211658 24.262010502688568 27.85710576454457 21.977122575555203 14-15 22.704911290985887 25.652411617566656 26.546694599402315 25.095982492045138 16-17 21.528773094460554 28.817203329953532 27.39755896517206 22.256464610413854 18-19 21.468083109353174 27.821771032874043 29.269753179400126 21.44039267837266 20-21 22.435377436540207 26.641162011115522 29.138348618171694 21.785111934172573 22-23 22.07154742901525 26.514418215497678 29.182037248064653 22.231997107422423 24-25 22.25481711897596 26.83143091310043 29.18913322338872 21.72461874453489 26-27 22.015795641071374 26.56088072665368 29.688270578570346 21.735053053704597 28-29 21.62388814936641 27.114576455747404 29.565284424430928 21.69625097045526 30-31 21.884471715926175 26.769066966331856 29.254803249569644 22.091658068172325 32-33 21.810560682039345 26.855283066519288 29.299056332045197 22.03509991939617 34-35 22.33810435940149 26.711101413051384 29.15710905140235 21.793685176144773 36-37 22.41743254803344 26.383457220531252 29.384877805203136 21.814232426232174 38-39 21.971760653465335 26.98168737684339 29.30047124718001 21.746080722511266 40-41 21.99427115428935 26.85766152072614 29.369647955480087 21.77841936950442 42-43 21.696817239982348 27.051540998242768 28.79935606813215 22.452285693642736 44-45 22.019066658474 26.86728701928357 28.76922756858254 22.344418753659887 46-47 22.453734502302087 27.245096867617242 28.28193493002944 22.019233700051235 48-49 22.23476385339548 27.21710289287533 28.281243901880472 22.266889351848715 50-51 21.951355217545217 27.352743617015275 28.575902745915155 22.119998419524357 52-53 22.211547796715788 27.693817696007773 27.960674749842678 22.13395975743376 54-55 22.051904479140898 27.318730891264174 28.060308694279257 22.569055935315667 56-57 22.198968567732212 27.519305084685623 27.83907553633478 22.44265081124738 58-59 22.386700439273977 27.165526524000732 28.019125625777537 22.428647410947754 60-61 22.18044633804082 27.360102261945602 28.403865013844747 22.055586386168834 62-63 22.002942249404825 27.198679890554256 28.519515061046352 22.278862798994563 64-65 21.854079922176304 27.59878671410661 28.247199259694337 22.29993410402275 66-67 22.00782568419097 27.383121139088296 27.81940093616626 22.789652240554474 68-69 21.756630112742116 27.35699756040171 28.236959228695362 22.64941309816081 70-71 22.19206611900789 27.2663013099493 27.94178977915067 22.59984279189214 72-73 22.122041744332655 26.952401165159145 28.061808525427303 22.8637485650809 74-75 21.708072220256494 27.94267677606618 27.611117329049083 22.738133674628244 76-77 21.90006744802167 27.327429061296964 27.837221135048313 22.935282355633056 78-79 21.91932417876518 27.317059560923273 27.951133849071386 22.81248241124016 80-81 22.03026175440249 27.420606518814495 27.74029633438042 22.808835392402596 82-83 21.981670450649265 27.259737532774537 27.988671597939458 22.76992041863674 84-85 21.846121194742913 27.29852348880723 27.75547204524394 23.099883271205922 86-87 21.652920326681844 27.273928362900147 27.97828258641701 23.094868724001007 88-89 21.815323924423303 27.136857295317974 27.705931008742247 23.341887771516475 90-91 21.701563190801973 26.96002467548692 27.94765909766172 23.390753036049386 92-93 21.879730314232365 27.1590230648227 27.760294083020977 23.200952537923953 94-95 21.693960905691583 26.91803406648735 27.854380264931468 23.533624762889595 96-97 21.40251199065906 26.768537542777704 28.03524140978541 23.793709056777825 98-99 21.47069571844976 27.077806401795414 27.61058513089978 23.840912748855047 100-101 21.57352191022643 26.346362476815077 28.2633268657725 23.816788747186 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1018.0 1 713.5 2 489.5 3 606.5 4 554.0 5 563.5 6 964.5 7 1893.0 8 3057.0 9 3696.5 10 3415.5 11 3023.0 12 3114.0 13 3308.0 14 3587.0 15 3841.5 16 4097.5 17 4365.0 18 4632.5 19 5061.5 20 5767.0 21 6871.5 22 8262.0 23 10025.5 24 11757.0 25 14534.5 26 19528.0 27 25008.0 28 28572.5 29 31927.0 30 36663.0 31 42735.5 32 49612.5 33 56612.0 34 64218.5 35 71129.0 36 79679.0 37 87870.0 38 92230.0 39 94295.0 40 94605.5 41 95007.5 42 94513.5 43 95197.0 44 98373.5 45 100986.5 46 103575.0 47 113676.5 48 138536.0 49 137425.0 50 113279.0 51 103675.5 52 101005.0 53 100397.5 54 98038.5 55 93069.5 56 86616.0 57 79207.0 58 72401.0 59 65271.5 60 55013.0 61 44267.5 62 34843.0 63 26158.5 64 19139.0 65 13946.0 66 9768.0 67 7445.0 68 5446.5 69 3654.0 70 2613.0 71 1601.0 72 980.0 73 689.5 74 474.0 75 301.0 76 179.5 77 78.0 78 39.5 79 21.0 80 12.0 81 9.0 82 6.0 83 5.0 84 4.0 85 4.0 86 2.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40301914397379135 2 0.0 3 0.0 4 0.0 5 4.5156206607707716E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001419195064813671 16-17 0.0021126653805748964 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002338446413613435 36-37 0.006999212024194695 38-39 0.012369575167182793 40-41 0.002370700846904655 42-43 6.773430991156157E-4 44-45 4.999437160139069E-4 46-47 3.0641711626658806E-4 48-49 2.580354663297584E-4 50-51 5.321981493051266E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.935265997473188E-4 60-61 0.0024352097134870943 62-63 0.0 64-65 2.580354663297584E-4 66-67 0.0014514494981048907 68-69 0.001403067848168061 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.1254858727194906 78-79 8.063608322804948E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.15083785728638938 88-89 0.44646586561706436 90-91 0.3432839335184523 92-93 0.0 94-95 0.0 96-97 6.128342325331761E-4 98-99 0.0 100-101 1.451449498104891E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3100349.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.21831350315493 #Duplication Level Percentage of deduplicated Percentage of total 1 69.27667151302114 29.24744236391809 2 15.032034330759252 12.692542759323638 3 5.901325693164559 7.474320545947332 4 2.9325147880830853 4.952233147037186 5 1.7101047386959347 3.6098868990747905 6 1.0673101264333973 2.703602011371426 7 0.7411554511443227 2.190323323368827 8 0.5223582520288635 1.764246755609166 9 0.402307912263716 1.528628540827438 >10 2.1373076639483664 16.451057136529617 >50 0.17279879287665834 5.071638252119605 >100 0.09631598437681636 7.468807295620001 >500 0.0052729212850880745 1.5048695846841518 >1k 0.0021397361735242732 1.9148016861040018 >5k 2.2925744716331497E-4 0.6834890264551521 >10k+ 1.5283829810887664E-4 0.7421106720095072 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11779 0.37992496973727796 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11223 0.36199150482735976 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9539 0.30767503916494565 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6068 0.1957199012111217 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5578 0.179915228898424 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4470 0.14417731681175247 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4160 0.13417844249147434 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3954 0.12753402923348306 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3532 0.11392265838458832 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3244 0.104633381596717 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.22544332912198E-5 0.0 2 0.0 0.0 0.0 3.22544332912198E-5 0.0 3 0.0 0.0 0.0 9.676329987365939E-5 0.0 4 0.0 0.0 0.0 9.676329987365939E-5 0.0 5 0.0 0.0 0.0 9.676329987365939E-5 0.0 6 0.0 0.0 0.0 9.676329987365939E-5 6.45088665824396E-5 7 0.0 0.0 0.0 9.676329987365939E-5 6.45088665824396E-5 8 0.0 0.0 0.0 9.676329987365939E-5 6.45088665824396E-5 9 0.0 0.0 0.0 2.9028989962097813E-4 6.45088665824396E-5 10-11 1.6127216645609897E-4 0.0 0.0 4.1930763278585735E-4 1.1289051651926929E-4 12-13 1.9352659974731877E-4 0.0 0.0 5.160709326595168E-4 2.0965381639292868E-4 14-15 1.9352659974731877E-4 0.0 0.0 5.805797992419563E-4 3.5479876620341774E-4 16-17 1.9352659974731877E-4 0.0 0.0 6.773430991156157E-4 4.515620660770771E-4 18-19 1.9352659974731877E-4 0.0 0.0 8.386152655717146E-4 4.838164993682969E-4 20-21 1.9352659974731877E-4 0.0 0.0 0.001080523515255863 5.483253659507365E-4 22-23 1.9352659974731877E-4 0.0 0.0 0.0013224317649400117 5.967070158875661E-4 24-25 2.2578103303853855E-4 0.0 0.0 0.0016449760978522097 6.773430991156157E-4 26-27 2.5803546632975833E-4 0.0 0.0 0.002225555897094166 7.095975324068355E-4 28-29 2.9028989962097813E-4 0.0 0.0 0.005096200460012728 7.095975324068355E-4 30-31 2.9028989962097813E-4 0.0 0.0 0.013579116415603533 7.095975324068355E-4 32-33 3.2254433291219794E-4 0.0 0.0 0.028706445629185617 7.418519656980552E-4 34-35 3.5479876620341774E-4 0.0 0.0 0.055009935978175356 7.741063989892751E-4 36-37 3.8705319949463755E-4 0.0 0.0 0.09631173780758231 7.741063989892751E-4 38-39 3.8705319949463755E-4 0.0 0.0 0.15553087733026186 7.741063989892751E-4 40-41 4.1930763278585735E-4 0.0 0.0 0.2203300338123224 7.741063989892751E-4 42-43 4.838164993682969E-4 0.0 0.0 0.2895964293052169 7.741063989892751E-4 44-45 4.999437160139069E-4 0.0 0.0 0.3763286004253069 7.741063989892751E-4 46-47 5.483253659507365E-4 0.0 0.0 0.48207475997057103 7.741063989892751E-4 48-49 6.28961449178786E-4 0.0 0.0 0.5957393828888295 8.063608322804948E-4 50-51 6.450886658243959E-4 0.0 0.0 0.7162419456648268 8.224880489261048E-4 52-53 6.450886658243959E-4 0.0 0.0 0.8373734698900027 9.031241321541543E-4 54-55 6.450886658243959E-4 0.0 0.0 0.974906373443764 9.353785654453741E-4 56-57 6.450886658243959E-4 0.0 0.0 1.143451914607033 9.676329987365938E-4 58-59 6.450886658243959E-4 0.0 0.0 1.3275763470499613 9.998874320278136E-4 60-61 6.450886658243959E-4 0.0 0.0 1.5308115312179371 0.0010321418653190335 62-63 6.450886658243959E-4 0.0 0.0 1.740529856477448 0.001096650731901473 64-65 6.450886658243959E-4 0.0 0.0 1.9606663636900232 0.0011611595984839125 66-67 6.450886658243959E-4 0.0 0.0 2.210831748296724 0.0011611595984839125 68-69 6.450886658243959E-4 0.0 0.0 2.506008194561322 0.0011611595984839125 70-71 6.450886658243959E-4 0.0 0.0 2.8250529214614226 0.0012256684650663523 72-73 6.450886658243959E-4 0.0 0.0 3.151725821834897 0.001257922898357572 74-75 6.450886658243959E-4 0.0 0.0 3.4936228147218267 0.001257922898357572 76-77 6.450886658243959E-4 0.0 0.0 3.8643713981877523 0.001257922898357572 78-79 6.450886658243959E-4 0.0 0.0 4.28845268710071 0.001257922898357572 80-81 6.450886658243959E-4 0.0 0.0 4.759980247385052 0.001257922898357572 82-83 6.450886658243959E-4 0.0 0.0 5.237378114528397 0.0012901773316487918 84-85 6.450886658243959E-4 0.0 0.0 5.730371000168045 0.0012901773316487918 86-87 6.450886658243959E-4 0.0 0.0 6.255602191882269 0.0013063045482944015 88-89 6.450886658243959E-4 0.0 0.0 6.818216271781016 0.0013546861982312315 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11675 0.0 23.958702 1 TACATGG 11800 0.0 23.24358 2 ACATGGG 11750 0.0 22.413633 3 GAGTACT 10625 0.0 21.237322 12-13 GTACTTT 11270 0.0 20.16925 14-15 CATGGGG 5795 0.0 19.898026 4 AGTACTT 11430 0.0 18.682905 12-13 GTATACG 155 9.4249693E-4 18.445974 1 CATGGGA 6280 0.0 17.832388 4 ACTTTTT 13525 0.0 16.735483 16-17 AGTACAT 8915 0.0 15.968239 2 AGAGTAC 21265 0.0 15.933493 10-11 GTATCAA 31025 0.0 15.451415 1 GTATAGG 1080 0.0 15.442811 1 TACTTTT 13560 0.0 15.415739 14-15 GTGTAGC 1640 0.0 15.399765 1 CGTATAG 280 1.2992017E-5 15.316747 1 GGTATCA 26225 0.0 14.500049 1 TTTGCGT 695 0.0 14.3381 3 GTGTAGG 1265 0.0 14.314465 1 >>END_MODULE